Classifications

• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
  • A61K36/18—Magnoliophyta (angiosperms)
  • A61K36/185—Magnoliopsida (dicotyledons)
  • A61K36/85—Verbenaceae (Verbena family)
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23K—FODDER
  • A23K20/00—Accessory food factors for animal feeding-stuffs
  • A23K20/10—Organic substances
  • A23K20/111—Aromatic compounds
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23K—FODDER
  • A23K50/00—Feeding-stuffs specially adapted for particular animals
  • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23K—FODDER
  • A23K50/00—Feeding-stuffs specially adapted for particular animals
  • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
  • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
  • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
  • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
  • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P37/00—Drugs for immunological or allergic disorders
  • A61P37/02—Immunomodulators
  • A61P37/04—Immunostimulants
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P39/00—General protective or antinoxious agents
  • A61P39/06—Free radical scavengers or antioxidants

Definitions

• the present invention relates to a process for extracting active principles with antioxidant activity. Furthermore, the present invention relates to the extracts thus obtained and their use for preparing drugs or nutritional substances.
• Figure 1 is a schematic diagram, representing the phases of the claimed process
• Figure 2 is a schematic diagram, representing the phases of a variant of the claimed process
• Figure 3 is a diagram illustrating the average antiradical effectiveness of the verbascoside, of the extract of Lippia polystachya and of the Trolox according to the concentration of the active principle (increase percentage of T1/2 compared with the control blood, calculated as average of three independent tests);
• Figure 4 and 5 are diagrams illustrating respectively the concentration of serum IgA and of serum IgG in blood samples taken at 0 and 56 days during experimentation;
• Figure 6 and 7 are diagrams illustrating respectively the concentration of IgA and IgG in colostrum samples taken during experimentation;
• Figure 8 is a diagram illustrating the percentage variation of average daily gain (ADG), feed intake (Fl) and feed conversion (FC) of treated piglets with respect to the Control.
• ADG average daily gain
• Fl feed intake
• FC feed conversion
• Figure 9 is a diagram illustrating the curve of growth of broilers fed with control diet or integrated with extract of Lippia polystachya and a theoretical curve of growth.
• the aim of the present invention is to overcome the cited drawbacks by devising a process for extracting active principles, in particular verbascoside and isoverbascoside, from vegetable tissues, in order to allow its use in zootechnical, pharmaceutical and alimentary field.
• the process according to the invention provides for extracting said active principles with antioxidant activity, in particular for pharmaceutical, zootechnical and nutritional use, from the leaves of a vegetable species, the Lippia polystachya, of the family of the Verbenaceae, using different mixtures of methanol, ethanol, butanol and water to obtain antioxidant active principles of the polyphenol type, in particular with presence of verbascoside and isoverbascoside having high antioxidant activity.
• the process provides for macerating the leaves of Lippia polystachya with different solvents so as to be able to extract the fraction of the polyphenols.
• the leaves and the solvent are in a prearranged quantitative ratio.
• the percentage of leaves is between 5 and 20%, the solvent percentage is included between 80 and 95%.
• the extraction after maceration in a solution of ethanol comprised between 50 and 70% turned out advantageous.
• the use of ethanol and water is more reliable and safe than the other organic solvents that can be used.
• the extraction takes place at a temperature comprised between 20 and 40 0 C, preferably through ultrasounds. Through centrifugation it is then possible to separate the concentrated extract from the drained leaves.
• the extract may be mixed with water and maltodextrines in a quantitative ratio established according to the desired percentage of glycosidic phenyl-propanoids, for instance in a 1 :1 ratio with respect to the dry extract. Finally, this mixture is dried through spray-drying in order to obtain a final extract with high-yield in phenyl-propanoids, in particular verbascoside and isoverbascoside. Alternatively, the extract can be used as it is.
• the process provides, in this case, for steam-extracting the volatile oil, and thus the fraction rich in phenyl-propanoids through the use of suitable solvent, for instance ethanol and water.
• suitable solvent for instance ethanol and water.
• the concentrated extract is mixed with water, maltodextrines and, differently from the process previously described, with the volatile oil recovered from the cited steam-distillation.
• the mixture thus formed is spray- dried in order to obtain the final extract with high-yield in active principles with antioxidant activity, in particular verbascoside and isoverbascoside.
• the antioxidant capacity of the final extracts obtained through the extracting process according to the invention has been evaluated through the KRL test, which allows to evaluate the global resistance of the blood facing a radical attack (Prost, M. 1989, patent FR 2,642,526; Prost, M. 1992, patent US 5,135,850).
• the intra- and extra-cellular antioxidant defences contribute to maintain the integrity of the membrane and the cellular functions until the cellular lysis.
• the global potential of antiradical defence is expressed as time required for 50% of the blood cells to be lysed (T Vi in minutes).
• the test also allows to determine in vitro the antioxidant capacity or the pro- oxidant activity of different compounds (Stocher, et al., 2000. Ann. FaIs. Exp.Chim. 949:471 -483). In fact, by adding to the blood a substance with antiradical activity it is observed an increase of the global potential of defence against the radical aggression, compared with a control blood, with no added substances.
• the Trolox water-soluble analogous of the vitamin E, having PM 250.29 g/mol
• the range of concentrations between 0 and 1000 ⁇ mol/L in each experimentation.
• the expression of the antiradical effectiveness of the products has been expressed in equivalent mmol of Trolox per gram of product and per gram of active principle.
• Table 1 Average antiradical capacity of the verbascoside and of the extract of Lippia polystachya (increase percentage of the T1/2 compared with the control blood, obtained as average of 3 independent tests).
• Lippia polystachya extract has, related to the present dose of verbascoside, an antiradical power higher than the verbascoside in purity, thus highlighting a probable synergic action between various active principles contained in the extract.
• the antioxidant capacity turns out to be 16.8 and 38.2 mM of Trolox per gram of active principle respectively for the verbascoside and the Lippia polystachya extract (see figure 3).
• the pig has been chosen as animal model considering the particular immune status present in post-weaning, in which the passive covering due to the immunoglobulins received from the mother through the colostrum is exhausting, and the defence system of the young animal is not yet completely efficient.
• the second test carried out upon the sow aimed to verify the possibility of a modulation of the immune response of the reproducer for the positive effects it can have on the passive immunity transmitted to the newborn.
• Test I pigs in post-weaning
• Test II influence of the Lippia polystachya extract on the modulation of the immune system of the sow
• the aim of the present research is to evaluate the effects of the administration of the Lippia polystachya extract, rich in phenyl-propanoids glucosides, on growth performance and wellness.
• Test I pigs in post-weaning
• the animals have been fed ad libitum with isoenergetic and isoproteic diets suitable to satisfy the requirements in the various phases of growth.
• the experimental feedstuff has been integrated with the inclusion of 750 g/t of Lippia polystachya extract in order to bring 3.75 mg of verbascoside per kg of feed.
• the control group contained lincomycin (100 mg/t) while the treated group was medication-free.
• the animals have been fed ad libitum for the whole period with isoenergetic and isoproteic diets.
• the experimental diet has been integrated with the inclusion of 700 g/t of extract of Lippia polystachya in order to bring 3.5 mg of verbascoside per kg of foodstuff.
• the process according to the claimed invention reaches the aim to allow the extraction of active principles, in particular verbascoside and isoverbascoside, from vegetable tissues, in order to obtain a final extract suitable to be profitably employed in zootechnical, pharmaceutical and alimentary field.
• the final extract obtainable from the process according to the claimed invention can usefully be used in the preparation of drugs and dietary supplements, as antioxidant, immunostimulant and immunomodulant, or as promoter of growth and wellness, or to improve the nutritional and technological quality of the meats.

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• Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Polymers & Plastics (AREA)
• Engineering & Computer Science (AREA)
• Natural Medicines & Medicinal Plants (AREA)
• Food Science & Technology (AREA)
• Pharmacology & Pharmacy (AREA)
• Animal Husbandry (AREA)
• Zoology (AREA)
• Immunology (AREA)
• Veterinary Medicine (AREA)
• Public Health (AREA)
• General Health & Medical Sciences (AREA)
• Medicinal Chemistry (AREA)
• Animal Behavior & Ethology (AREA)
• Birds (AREA)
• Mycology (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Organic Chemistry (AREA)
• Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
• Botany (AREA)
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• Alternative & Traditional Medicine (AREA)
• Bioinformatics & Cheminformatics (AREA)
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• Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
• Treatment Of Liquids With Adsorbents In General (AREA)
• Extraction Or Liquid Replacement (AREA)

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Citations (5)

• 2008
  • 2008-04-04 IT IT000212A patent/ITBO20080212A1/it unknown
• 2009
  • 2009-03-27 WO PCT/IB2009/051298 patent/WO2009122337A1/en active Application Filing

Patent Citations (5)

Non-Patent Citations (15)

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