WO2009107614A3 - Method for detection of target nucleic acid and device for detection of target nucleic acid - Google Patents

Method for detection of target nucleic acid and device for detection of target nucleic acid Download PDF

Info

Publication number
WO2009107614A3
WO2009107614A3 PCT/JP2009/053311 JP2009053311W WO2009107614A3 WO 2009107614 A3 WO2009107614 A3 WO 2009107614A3 JP 2009053311 W JP2009053311 W JP 2009053311W WO 2009107614 A3 WO2009107614 A3 WO 2009107614A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
target nucleic
detection
detecting
sample
Prior art date
Application number
PCT/JP2009/053311
Other languages
French (fr)
Other versions
WO2009107614A2 (en
Inventor
Masayoshi Takahashi
Nobuhiro Gemma
Original Assignee
Kabushiki Kaisha Toshiba
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kabushiki Kaisha Toshiba filed Critical Kabushiki Kaisha Toshiba
Publication of WO2009107614A2 publication Critical patent/WO2009107614A2/en
Publication of WO2009107614A3 publication Critical patent/WO2009107614A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention provides a method of detecting a target nucleic acid, including a step of supplying a nucleic acid sample to a device for detection of a target nucleic acid including a support, a plurality of nucleic acid probes complementary to a plurality of target nucleic acids different from one another, and a detection compartment having the plurality of nucleic acid probes immobilized in different amounts on the same region, a step of hybridizing the nucleic acid sample with the nucleic acid probe to give a hybrid chain, a step of detecting the amount of a signal derived from the hybrid chain in the detection compartment, and a step of judging, from the signal amount, the presence or absence of the target nucleic acid in the nucleic acid sample.
PCT/JP2009/053311 2008-02-27 2009-02-18 Method for detection of target nucleic acid and device for detection of target nucleic acid WO2009107614A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2008046425A JP4580996B2 (en) 2008-02-27 2008-02-27 Target nucleic acid detection method and target nucleic acid detection substrate
JP2008-046425 2008-02-27

Publications (2)

Publication Number Publication Date
WO2009107614A2 WO2009107614A2 (en) 2009-09-03
WO2009107614A3 true WO2009107614A3 (en) 2009-11-12

Family

ID=40674214

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2009/053311 WO2009107614A2 (en) 2008-02-27 2009-02-18 Method for detection of target nucleic acid and device for detection of target nucleic acid

Country Status (2)

Country Link
JP (1) JP4580996B2 (en)
WO (1) WO2009107614A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011102729A (en) * 2009-11-10 2011-05-26 Sharp Corp Analyzing chip device, chemical sensor chip housing adaptor used analyzing chip device analyzer, and analyzing method using the analyzing chip device
EP2572001A2 (en) * 2010-05-19 2013-03-27 QIAGEN Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
US10815521B1 (en) * 2017-03-13 2020-10-27 Ravi Saraf Electrochemical microarray chip and applications thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060127924A1 (en) * 2004-08-10 2006-06-15 Becton, Dickinson And Company Method for rapid identification of microorganisms
US20070092882A1 (en) * 2005-10-21 2007-04-26 Hui Wang Analysis of microRNA

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003020952A2 (en) * 2001-08-31 2003-03-13 Gen-Probe Incorporated Affinity-shifted probes for quantifying analyte polynucleotides
CN101307363A (en) * 2002-07-26 2008-11-19 株式会社东芝 Nucleic acid probe-immobilized substrate and method of detecting the presence of target nucleic acid by using the same

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060127924A1 (en) * 2004-08-10 2006-06-15 Becton, Dickinson And Company Method for rapid identification of microorganisms
US20070092882A1 (en) * 2005-10-21 2007-04-26 Hui Wang Analysis of microRNA

Also Published As

Publication number Publication date
JP2009201402A (en) 2009-09-10
JP4580996B2 (en) 2010-11-17
WO2009107614A2 (en) 2009-09-03

Similar Documents

Publication Publication Date Title
WO2010013138A3 (en) Methods and compositions for detection of a pathogen, disease, medical condition, or biomarker thereof
AU2009324884A8 (en) Compositions and methods for detecting small RNAs, and uses thereof
WO2009022125A8 (en) Identification of nucleic acid sequences
WO2011062933A3 (en) Array-based proximity ligation association assays
ATE544869T1 (en) METHOD AND SUBSTANCES FOR ISOLATION AND DETECTION OF SMALL POLYNUCLEOTIDES
IN2012DN03156A (en)
WO2009123996A3 (en) Hybridization quantitation method for modified micro-rna and -dna based oligonucleotides
WO2010129937A3 (en) Methods for detecting genetic variations in dna samples
WO2011053871A3 (en) Multi-primer assay for mycoplasma detection
WO2007011946A3 (en) Detection of nucleic acid amplification
WO2009105648A3 (en) Kits, solid supports and methods for point-of-care amplification and detection of polynucleotides
WO2011146629A3 (en) Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
NZ594751A (en) Method of diagnosis of infection by mycobacteria and reagents therefor
WO2008137466A3 (en) Nucleic acid quantitation methods
WO2008014485A3 (en) Multiplex q-pcr arrays
WO2010077304A3 (en) Methods for the characterization of microorganisms on solid or semi-solid media
WO2006088911A3 (en) Sequence-specific detection of nucleotide sequences
WO2008054556A3 (en) Methods and compositions for detection of a target nucleic acid sequence
WO2010096202A3 (en) Sequence-specific detection of nucleotide sequences
WO2011087789A3 (en) Methods for the detection of microorganisms
WO2006119326A3 (en) Oligonucleotide probe/primer compositions and methods for polynucleotide detection
WO2013009084A3 (en) Composition for simultaneously detecting mycobacterium tuberculosis and nontuberculous mycobacteria by means of real-time multiplex polymerase chain reaction comprising nested hybridization pna probe system having parallel binding structure, and method for detection using same
WO2009057829A3 (en) Nucleic acid primer set for detection of drug-resistant strain of hepatitis b virus, assay kit, and method of detecting drug-resistant strain of hepatitis b virus
EP2301946A4 (en) Nucleoside triphosphate derivative, nucleic acid probe, multilabeled nucleic acid probe, method for production of multilabeled nucleic acid probe, and method for detection of target nucleic acid
WO2010130877A3 (en) Method for detecting nucleic acids

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 09713703

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 09713703

Country of ref document: EP

Kind code of ref document: A2