WO2009104896A3 - 암 치료용 핵산 의약 조성물 - Google Patents

암 치료용 핵산 의약 조성물 Download PDF

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Publication number
WO2009104896A3
WO2009104896A3 PCT/KR2009/000762 KR2009000762W WO2009104896A3 WO 2009104896 A3 WO2009104896 A3 WO 2009104896A3 KR 2009000762 W KR2009000762 W KR 2009000762W WO 2009104896 A3 WO2009104896 A3 WO 2009104896A3
Authority
WO
WIPO (PCT)
Prior art keywords
seq
sirna
nucleic
pharmaceutical composition
cancer therapy
Prior art date
Application number
PCT/KR2009/000762
Other languages
English (en)
French (fr)
Other versions
WO2009104896A2 (ko
Inventor
오유경
심가용
김상희
Original Assignee
고려대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 고려대학교 산학협력단 filed Critical 고려대학교 산학협력단
Priority to US12/735,809 priority Critical patent/US20110160282A1/en
Publication of WO2009104896A2 publication Critical patent/WO2009104896A2/ko
Publication of WO2009104896A3 publication Critical patent/WO2009104896A3/ko

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1135—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against oncogenes or tumor suppressor genes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/315—Phosphorothioates

Abstract

본 발명은 서열번호 1의 센스서열 및 서열번호 2의 안티센스서열을 갖는 siRNA, 서열번호 3의 센스서열 및 서열번호 4의 안티센스서열을 갖는 siRNA, 및 서열번호 5의 센스서열 및 서열번호 6의 안티센스서열을 갖는 siRNA 중에서 선택되며, 세포 내에서 Mcl-1의 발현을 억제하는 하나 이상의 siRNA(small interfering RNA, siRNA) 및 이를 포함하는 암 치료용 핵산 의약 조성물을 제공한다. 본 발명의 siRNA는 리보핵산 매개 간섭현상(RNA-mediated interference, RNAi)에 의해 암세포에 공통적으로 발현되는 Mcl-1의 발현을 억제하여 암세포를 사멸시키므로 본 발명의 조성물은 우수한 항암제로 이용될 수 있다.
PCT/KR2009/000762 2008-02-19 2009-02-18 암 치료용 핵산 의약 조성물 WO2009104896A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US12/735,809 US20110160282A1 (en) 2008-02-19 2009-02-18 Nucleic-acid pharmaceutical composition for cancer therapy

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2008-0014724 2008-02-19
KR1020080014724A KR100870314B1 (ko) 2008-02-19 2008-02-19 암 치료용 핵산 의약 조성물

Publications (2)

Publication Number Publication Date
WO2009104896A2 WO2009104896A2 (ko) 2009-08-27
WO2009104896A3 true WO2009104896A3 (ko) 2009-11-05

Family

ID=40284710

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/000762 WO2009104896A2 (ko) 2008-02-19 2009-02-18 암 치료용 핵산 의약 조성물

Country Status (3)

Country Link
US (1) US20110160282A1 (ko)
KR (1) KR100870314B1 (ko)
WO (1) WO2009104896A2 (ko)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101085203B1 (ko) 2011-04-01 2011-11-21 서울대학교산학협력단 의약 전달용 인지질 나노입자
ES2803581T3 (es) 2014-10-29 2021-01-28 Walter & Eliza Hall Inst Medical Res Inhibidores de Mcl-1 para su uso en el tratamiento de enfermedades provocadas por neovascularización patológica
UA125142C2 (uk) * 2017-01-06 2022-01-19 Ле Лаборатуар Сервьє Комбінація інгібітору mcl-1 і таксанової сполуки, її застосування та фармацевтична композиція
CN109125741B (zh) * 2018-08-13 2022-02-11 四川大学 透明质酸/dotap/生存素编码基因自组装的三元复合物制剂及其制备方法
JP2023554111A (ja) * 2020-12-16 2023-12-26 インダストリー－アカデミック コーオペレイション ファウンデーション キョンサン ナショナル ユニバーシティ 遺伝子発現および抑制が同時に可能な核酸構造体

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006113679A2 (en) * 2005-04-15 2006-10-26 Board Of Regents, The University Of Texas System Delivery of sirna by neutral lipid compositions
US20070093653A1 (en) * 2002-11-14 2007-04-26 Dharmacon Inc. siRNA targeting MCL1

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004313167A (ja) * 2003-02-24 2004-11-11 Joji Inasawa 薬剤耐性マーカーおよびその利用
US7632824B2 (en) * 2006-03-16 2009-12-15 Isis Pharmaceuticals, Inc. Compositions and methods for modulation of Mcl-1 expression

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070093653A1 (en) * 2002-11-14 2007-04-26 Dharmacon Inc. siRNA targeting MCL1
WO2006113679A2 (en) * 2005-04-15 2006-10-26 Board Of Regents, The University Of Texas System Delivery of sirna by neutral lipid compositions

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHETOUI, N. ET AL.: "Down-Regulation of Mcl- by Small Interfering RNA Sensitizes Resistant Melanoma Cells to Fas-Mediated Apoptosis", MOL. CANCER RES., vol. 6, no. 1, January 2008 (2008-01-01), pages 42 - 52 *
HENNING, S.-B. ET AL.: "Suppression of Mcl-1 via RNA Interference Sensitizes Human Hepatocellular Carcinoma Cells towards Apoptosis Induction", BMC CANCER, vol. 6, no. 232, 2 October 2006 (2006-10-02) *
HUSSAIN, S.-R. A. ET AL.: "Mcl-1 Is a Relevant Therapeutic Target in Acute and Chronic Lymphoid Malignancies: Down-Regulation Enhances Rituximab-Mediated Apoptosis and Complement-Dependent Cytotoxicity", CLIN. CANCER RES., vol. 13, no. 7, 1 April 2007 (2007-04-01), pages 2144 - 2150 *

Also Published As

Publication number Publication date
KR100870314B1 (ko) 2008-11-25
US20110160282A1 (en) 2011-06-30
WO2009104896A2 (ko) 2009-08-27

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