WO2009093367A1 - Procédé de production d'éthanol - Google Patents
Procédé de production d'éthanol Download PDFInfo
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- WO2009093367A1 WO2009093367A1 PCT/JP2008/069523 JP2008069523W WO2009093367A1 WO 2009093367 A1 WO2009093367 A1 WO 2009093367A1 JP 2008069523 W JP2008069523 W JP 2008069523W WO 2009093367 A1 WO2009093367 A1 WO 2009093367A1
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- liquid mixture
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- plant
- yeast
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Definitions
- the present invention is a reaction in which a solid-liquid mixture containing sugar is fermented with yeast to produce ethanol, and carbon dioxide generated during the fermentation is photo-synthesized by light irradiation of a plant chloroplast and a light-emitting diode to produce sugar.
- the reaction and the reaction of fermenting the sugar obtained in the reaction with yeast to produce ethanol proceed in parallel in the same container.
- the present invention relates to a method for producing ethanol.
- Japanese Patent Application Laid-Open No. 07-087986 discloses “microalgae culture means 1” for culturing microalgae that accumulate starch in cells by photosynthesis, and “microalgae concentration” for concentrating a culture solution containing algal bodies cultured by this means.
- a method for producing ethanol comprising means 2 ”and“ holding means 3 ”for producing ethanol by maintaining the slurry obtained by the means in a dark and anaerobic atmosphere while maintaining the pH in the range of 6.0 to 9.0 is described.
- “microalgae culture means 1” and “holding means 3” are completely independent separate processes, and a separate process of “microalgae concentration means 2” is required between the two processes.
- the operation is complicated, and it cannot be said that the ethanol production method is always highly efficient.
- the “holding means 3” for generating ethanol needs to be kept in a dark and anaerobic atmosphere, it can be performed in parallel with the “microalgae culture means 1” that requires light irradiation in the same container. It was impossible.
- Japanese Patent Laid-Open No. 2007-325564 discloses a method for inoculating koji mold and saccharifying a raw material subjected to a specific treatment to produce ethanol using rice as a cereal as a starting material and adding yeast to perform ethanol fermentation.
- a method of synthesizing and utilizing industrial methanol or the like by simply reusing carbon dioxide generated as a by-product during ethanol fermentation without letting it escape to the atmosphere is described.
- the above method describes the reuse of carbon dioxide generated during ethanol fermentation, the carbon dioxide is converted into a sugar that is a raw material for ethanol fermentation by photosynthesis, and the sugar is converted into the above ethanol fermentation. There was no suggestion or description about performing in parallel in the same container.
- the present invention is a reaction in which a solid-liquid mixture containing sugar is fermented with yeast to produce ethanol, and carbon dioxide generated during the fermentation is photo-synthesized by light irradiation of a plant chloroplast and a light-emitting diode to produce sugar.
- the reaction and the reaction of fermenting the sugar obtained in the reaction with yeast to produce ethanol proceed in parallel in the same container.
- An object is to provide a method for producing ethanol.
- the present inventors have performed a simple operation of irradiating light from a light-emitting diode in a closed system containing a solid-liquid mixture containing sugar, yeast and plant chloroplasts.
- a reaction in which a solid-liquid mixture containing sugar is fermented with yeast to produce ethanol b) Reaction of photosynthesis of carbon dioxide generated during fermentation in a) by light irradiation of plant chloroplasts and light emitting diodes to sugar, and c) fermentation of sugar obtained in b) with yeast.
- the present inventors have found that the reaction for producing ethanol proceeds in parallel. Moreover, it discovered that ethanol was efficiently manufactured by irradiating the light of a specific wavelength range in this case.
- the present invention (1) A production process for producing ethanol by irradiating light from a light-emitting diode in a sealed system containing a solid-liquid mixture containing sugar, yeast and plant chloroplasts, and ethanol produced from the system.
- a method for producing ethanol comprising a recovery step of recovering outside, In the generating step, a) a reaction in which a solid-liquid mixture containing sugar is fermented with yeast to produce ethanol; b) Reaction of photosynthesis of carbon dioxide generated during fermentation in a) by light irradiation of plant chloroplasts and light emitting diodes to sugar, and c) fermentation of sugar obtained in b) with yeast.
- the reaction for producing ethanol proceeds in parallel, and the solid-liquid mixture containing sugar is saccharified by pyrolysis or hydrolysis of cellulose or hemicellulose, or rice, wheat, straw or corn containing starch Saccharify with koji, malt or enzyme, crush vegetables and / or fruits, or crush vegetables and / or fruits, then heat concentration method, freeze concentration method, reverse osmosis
- the plant chloroplast is prepared by concentrating by one or more concentration methods selected from a concentration method and a vacuum concentration method, and the plant chloroplast is a seed plant, a fern plant, an algae, a moss plant, a bacterium Or a mixture of these
- the method of producing ethanol is a plant chloroplast things, (2)
- the light of the light emitting diode is light whose total light emission amount in the wavelength regions of 380 to 520 nm and 620 to 780 nm is 70% or more of the light emission amount over the entire wavelength region.
- Production method (3) The method for producing ethanol according
- the total light emission amount in the wavelength range of 380 to 520 nm and 620 to 780 nm is 70% or more of the light emission amount over the entire wavelength region means that the light emission spectrum of the light emitting diode used is measured and recorded. It means that a value indicating the sum of the spectrum areas in the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total spectrum area as a percentage is 70% or more.
- the ethanol production method of the present invention produces sugar by a photosynthesis reaction using carbon dioxide generated in a sugar fermentation reaction as a raw material, and therefore does not discharge carbon dioxide. It can be said that this is a manufacturing method.
- ethanol is produced using sugar produced by the photosynthesis reaction as a raw material, the ethanol recovery rate is high compared to normal ethanol fermentation, and three reactions are simultaneously performed in one container. Since it can be performed, it is a very excellent ethanol production method that is simple and efficient in operation.
- additional carbon dioxide is added into the reaction system, whereby ethanol can be produced more efficiently.
- the method for producing ethanol of the present invention comprises a production step of producing ethanol by irradiating light from a light-emitting diode in a sealed system containing a solid-liquid mixture containing sugar, yeast and plant chloroplasts, and from the system. A recovery step of recovering the produced ethanol out of the system.
- the solid-liquid mixture containing sugar used in the present invention is saccharified by pyrolyzing or hydrolyzing cellulose or hemicellulose, or rice, wheat, straw or corn containing starch is saccharified using straw, malt or an enzyme agent.
- cellulose or hemicellulose include cellulose or hemicellulose obtained from a plant body such as wood.
- Saccharification of cellulose by thermal decomposition can be performed by a known method, for example, a method described in Japanese Patent No. 1400009.
- the saccharification of cellulose or hemicellulose by hydrolysis can be carried out by a known method, for example, a method of hydrolysis using dilute sulfuric acid described in JP-B 61-0444479.
- the solid-liquid mixture containing sugar is made of rice, wheat, straw or corn containing starch, and if necessary, subjected to a step of removing the hull, pulverization or pulverization, and the like. It can be prepared by adding.
- the enzyme agent include amylase.
- a solid-liquid mixture containing sugar is obtained by crushing vegetables and / or fruits, or crushing vegetables and / or fruits, followed by a heat concentration method, a freeze concentration method, a reverse osmosis concentration method, and a vacuum. It can prepare by concentrating by the 1 type (s) or 2 or more types of concentration method chosen from the concentration method.
- the vegetables are not limited as long as they contain sugar, but specific examples include radish, sugar beet, cabbage, lettuce, Chinese cabbage, sugar cane, tomato, carrot, onion, leek and the like.
- Fruits are not limited as long as they contain sugar, but specific examples include apples, tangerines, grapes, white peaches, yellow peaches, pears, pineapples, mangoes, bananas, melons, cherries, loquats, and blueberries. , Strawberry, kiwi, raspberry, blackberry, apricot, oyster, watermelon and the like.
- the vegetables and fruits are preferably washed before pulverization.
- a pulverization method a method usually used for pulverizing vegetables and / or fruits can be used, and examples thereof include pulverization using a mixer (for home use and business use).
- the solid-liquid mixture containing sugar prepared by pulverizing vegetables and / or fruits can be further concentrated.
- Concentration is particularly effective when the sugar content in the solid-liquid mixture is low.
- concentration method include a heat concentration method, a freezing concentration method, a reverse osmosis concentration method, a vacuum concentration method, and a concentration method in which two or more of these are performed simultaneously or continuously.
- concentration methods heat concentration method, freeze concentration method, reverse osmosis concentration method, vacuum concentration method
- concentration method are usually methods known in the technical field (heat concentration method, freeze concentration method, reverse osmosis concentration method, vacuum concentration method). Can be done.
- the solid-liquid mixture containing the saccharide prepared above can be sterilized as necessary.
- the yeast used in the present invention is not particularly limited as long as it can produce ethanol, and specific examples include wine yeast, sake yeast, whiskey yeast, beer yeast and the like.
- Plant chloroplasts used in the present invention include plant chloroplasts of seed plants, ferns, algae, moss plants, bacteria, or mixtures thereof. Although it does not specifically limit as a seed plant, For example, a ginkgo, rice, etc. are preferable. Although it does not specifically limit as a fern plant, For example, a cycad etc. are preferable. Although it does not specifically limit as algae, For example, kawamozuku, gray algae, crypt algae, wakame, euglena algae, chloracunion algae, cyanobacteria, uremo etc. are preferable.
- a moss plant For example, a sphagnum moss, a black moss, a scallop, a scale moss, a sphagnum, a hornbill, etc. are preferable.
- bacteria For example, cyanobacteria (extracted from a sea lion) etc. are preferable.
- Preferred plant chloroplasts include bacterial plant chloroplasts and cyanobacterial plant chloroplasts.
- the plant chloroplast used in the present invention preferably destroys the plant cell membrane so that the sugar produced by photosynthesis is efficiently fermented by the yeast in the solution.
- the destruction of the cell membrane of the plant can be performed, for example, by crushing the plant.
- the produced sugar is efficiently fermented without destroying the cell membrane, it is not necessary to destroy the cell membrane.
- the sealed system is not particularly limited as long as it is a sealed container that can be irradiated with light and can cope with a volume change caused by the generated gas (carbon dioxide, oxygen).
- a container etc. are mentioned.
- light irradiation can be performed from the outside and / or the inside of the glass container, and in the case of a metal container, light irradiation can be performed from the inside of the container.
- the said container is equipped with the expansion-contraction site
- the system is preferably stirrable.
- the glass container is, for example, a glass tube installed around a light emitting diode, and a solid-liquid mixture containing sugar, a solution containing yeast and plant chloroplasts are allowed to flow at a rate capable of an ethanol production reaction. It can also be a container capable of continuous reaction.
- the glass container is composed of, for example, two glass plates capable of irradiating light from a light-emitting diode from both the front and back surfaces, a solid-liquid mixture containing sugar between the glass plates, yeast and plant chloroplasts. It may also be a container capable of continuous reaction by flowing a solution containing
- the production step for producing ethanol is performed by irradiating the light of the light-emitting diode into the above-described sealed system.
- Three reactions of producing ethanol proceed in parallel in the closed system.
- Ethanol and carbon dioxide are produced by the fermentation of yeast in a) above.
- the produced carbon dioxide is converted to sugar by photosynthesis b) of the light of the plant chloroplast and the light emitting diode, and oxygen is generated at this time.
- the sugar is not particularly limited as long as it can be used for ethanol fermentation, and examples thereof include sucrose and glucose.
- the sugar produced in b) is fermented by yeast (reaction c)) to produce ethanol and carbon dioxide.
- the produced carbon dioxide is again ethanol through the photosynthesis of b) and alcohol fermentation of c). By repeating this cycle, almost all of the carbon dioxide is finally converted into ethanol and oxygen. Therefore, the progress of the reaction can be grasped by measuring the oxygen concentration in the gas present in the system.
- the reaction is preferably carried out until the oxygen concentration in the gas present in the system reaches 90% or more, and more preferably the reaction is carried out until the concentration reaches 95% or more.
- the light emitted from the light emitting diode used in the photosynthetic reaction is preferably light in which the total light emission amount in the wavelength regions of 380 to 520 nm and 620 to 780 nm is 70% or more of the light emission amount over the entire wavelength region, and more preferably , 80% or more of the light, and more preferably 90% or more of the light.
- the above% is less than 70, ethanol production efficiency tends to decrease.
- light having a wavelength shorter than 380 nm is not substantially contained, that is, the light emission amount is 5% or less, preferably 3% or less, and preferably 0%.
- the total light emission amount in the wavelength range of 380 to 520 nm and 620 to 780 nm is 70% or more of the light emission amount over the entire wavelength region means that the light emission spectrum of the light emitting diode used is measured and recorded. It means that a value indicating the sum of the spectrum areas in the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total spectrum area as a percentage is 70% or more.
- the manufacturing method of ethanol of this invention includes the collection
- the recovery step is not particularly limited as long as it is a method capable of separating ethanol from the reaction system.
- a known method such as a distillation method or a membrane separation method can be used.
- the container used for the distillation operation can be used as it is, and the reaction liquid can be transferred to another container for use.
- a preferred embodiment of the present invention is a method for producing ethanol in which additional carbon dioxide is added into the sealed system. By further adding carbon dioxide, the production efficiency of ethanol can be further improved.
- the additional addition of carbon dioxide is achieved, for example, by connecting a carbon dioxide introduction tube to the reaction vessel or placing it in an atmosphere of carbon dioxide. When adding carbon dioxide, the entire system is sealed so that the carbon dioxide does not flow out.
- the recovery step is achieved by distillation.
- the distillation method any of simple distillation, azeotropic distillation, azeotropic distillation with addition of benzene, and the like can be selected according to the required purity of ethanol.
- Production Example 1 Production of a solid-liquid mixture containing sugar (rice) 6 g of amylase and 315 g of water were added to 330 g of rice flour purchased from a food store, stirred with a mixer, boiled and kept at 70 ° C. to 80 ° C. for 12 hours. By saccharifying the rice flour, 651 g of a solid-liquid mixture (rice) containing sugar was prepared.
- Production Example 2 Production of sugar-containing solid-liquid mixture (wheat) Add 6 g of amylase and 315 g of water to 330 g of flour purchased from a food store, stir with a mixer, boil in water and keep at 70 ° C. to 80 ° C. for 12 hours. By saccharifying the wheat flour, 651 g of a solid-liquid mixture (wheat) containing sugar was prepared.
- Production Example 3 Production of Solid-Liquid Mixture Containing Sugar (Corn) 6 g of amylase and 315 g of water are added to 330 g of corn flour purchased from a food store, stirred with a mixer, boiled and kept at 70 ° C. to 80 ° C. for 12 hours. By saccharifying the corn flour, 651 g of a solid-liquid mixture (corn) containing sugar was prepared.
- Examples 1 to 10 and Comparative Example 1 217 g of the solid-liquid mixture (rice) containing sugar produced in Production Example 1 and 3 g of yeast were stirred with a mixer to prepare 220 g of a solid-liquid mixture (rice) containing sugar containing yeast.
- the prepared solid-liquid mixture (rice) containing sugar containing yeast is divided into 11 100 mL beakers of 20 g each, and 10 types of different plant pulverized products are added to each beaker by 1 g and stirred.
- Examples 11 to 20 and Comparative Example 2 217 g of the solid-liquid mixture (wheat) containing sugar produced in Production Example 2 and 3 g of yeast were stirred with a mixer to prepare 220 g of a solid-liquid mixture (wheat) containing sugar containing yeast.
- the prepared solid-liquid mixture (wheat) containing sugar containing yeast is divided into 11 100 mL beakers of 20 g each, and 10 types of different plant pulverized products are added to each beaker by 1 g and stirred, and then each solution is transparent glass.
- a solid-liquid mixture (wheat) containing sugar containing yeast without plant pulverized product was injected into a 100 mL syringe (Comparative Example 2).
- the eleven syringes prepared above contain light from a total of 600 light emitting diodes composed of 200 white light emitting diodes, 200 red light emitting diodes, 100 yellow light emitting diodes and 100 indigo light emitting diodes (36 watts in total). / Hour) for 24 hours.
- the oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter.
- the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was determined to be 77%.
- the solution in each syringe was distilled and the amount of alcohol recovered was measured. The above operation was performed three times in consideration of measurement errors.
- the results are summarized in Table 2.
- symbols a to j represent the same plants as shown above, and “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).
- Examples 21 to 30 and Comparative Example 3 217 g of the solid-liquid mixture (corn) containing saccharide produced in Production Example 3 and 3 g of yeast were stirred with a mixer to prepare 220 g of a solid-liquid mixture (corn) containing sugar containing yeast.
- the prepared solid-liquid mixture (corn) containing sugar containing yeast is divided into 11 100 mL beakers of 20 g each, 10 g of different plant pulverized products are added to each beaker by 1 g and stirred, and then each solution is transparent glass. It was injected into a 100 mL syringe, which is a manufactured container (Examples 21 to 30).
- the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 85%.
- the solution in each syringe was distilled and the amount of alcohol recovered was measured.
- the above operation was performed three times in consideration of measurement errors.
- the results are summarized in Table 3.
- symbols a to j represent the same plants as shown above, and “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).
- Examples 31 to 40 and Comparative Example 4 217 g of the solid-liquid mixture (corn) containing saccharide produced in Production Example 3 and 3 g of yeast were stirred with a mixer to prepare 220 g of a solid-liquid mixture (corn) containing sugar containing yeast.
- the prepared solid-liquid mixture (corn) containing sugar containing yeast was divided into 11 100 mL beakers of 20 g each, 10 g of different plant pulverized products were added to each beaker by 1 g and stirred, and then each beaker was sealed. I put it in the box.
- a fermentation experiment was conducted separately from the above experiment, and carbon dioxide released from the fermentation experiment was introduced into each beaker through a tube so as to be mixed at the liquid level of each beaker in the sealed box (Examples 31 to 40). ).
- a beaker containing 20 g of a solid-liquid mixture (corn) containing sugar containing yeast and not containing plant pulverized material was placed in the sealed box (Comparative Example 4).
- light of a total of 600 light emitting diodes composed of 200 white light emitting diodes, 200 red light emitting diodes, 100 orange light emitting diodes, and 100 purple light emitting diodes (36 watts / hour in total) 24 Irradiated for hours.
- the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas in the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 85%.
- the solution in each syringe was distilled and the amount of alcohol recovered was measured.
- the above operation was performed three times in consideration of measurement errors.
- the results are summarized in Table 4.
- symbols a to j represent the same plants as shown above, and “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).
- Production Example 4 Production of a solid-liquid mixture containing sugar (rice) 10 g of amylase (mixture of alpha amylase and glucoamylase) and 525 g of water were added to 530 g of rice flour purchased from a food store, stirred with a mixer, hot water roasted, and 70 ° C. Furthermore, 1065 g of a solid-liquid mixture (rice) containing sugar was prepared by saccharifying the rice flour over 12 hours while maintaining at 80 ° C.
- amylase mixture of alpha amylase and glucoamylase
- Examples 41 to 57 and Comparative Example 5 355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
- the prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred.
- Examples 58 to 74 and Comparative Example 6 355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
- the prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred.
- the emission spectrum of the light emitting diode was measured, and the ratio of the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 74%.
- the solution in each syringe was distilled and the amount of alcohol recovered was measured. The above operation was performed three times in consideration of measurement errors.
- the results are summarized in Table 6.
- the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).
- Examples 75 to 91 and Comparative Example 7 355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
- the prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred.
- the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 100%.
- the solution in each syringe was distilled and the amount of alcohol recovered was measured. The above operation was performed three times in consideration of measurement errors.
- the results are summarized in Table 7.
- the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).
- Examples 92 to 108 and Comparative Example 8 355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
- the prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred.
- the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 60%.
- the solution in each syringe was distilled and the amount of alcohol recovered was measured.
- the above operation was performed three times in consideration of measurement errors.
- the results are summarized in Table 8.
- the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).
- Examples 109 to 125 and Comparative Example 9 355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
- the prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred.
- the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 60%.
- the solution in each syringe was distilled and the amount of alcohol recovered was measured.
- the above operation was performed three times in consideration of measurement errors.
- the results are summarized in Table 9.
- the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).
- Examples 126 to 142 and Comparative Example 10 355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
- the prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred.
- Examples 143 to 159 and Comparative Example 11 355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
- the prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred.
- Examples 160 to 176 and Comparative Example 12 355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
- the prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred. It was injected into a 100 mL syringe, which is a manufactured container (Examples 160 to 176).
- Examples 177 to 193 and Comparative Example 13 355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
- the prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred.
- Examples 194 to 210 and Comparative Example 14 355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
- the prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred.
- Examples 211 to 227 and Comparative Example 15 355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
- the prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred.
- Examples 228 to 244 and Comparative Example 16 355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
- the prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred, and then each beaker was sealed. I put it in the box.
- a fermentation experiment was conducted separately from the above experiment, and carbon dioxide released from the fermentation experiment was introduced into each beaker through a tube so as to be mixed at the liquid level of each beaker in the sealed box (Examples 228 to 244). ).
- a beaker containing 20 g of a solid-liquid mixture (rice) containing sugar containing yeast without adding plant ground was placed in the sealed box (Comparative Example 16).
- Each beaker was irradiated with light from a light emitting diode composed of 600 white light emitting diodes (36 watts / hour in total) for 24 hours.
- the emission spectrum of the light-emitting diode was measured, and the ratio of the spectral area in the wavelength region of 380 to 520 nm and 620 to 780 nm to the total recorded spectral area was determined to be 80%.
- the solution in each syringe was distilled and the amount of alcohol recovered was measured. The above operation was performed three times in consideration of measurement errors.
- the results are summarized in Table 16.
- symbols a to j represent the same plants as shown above, and “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).
- the yield increased by 7 times, and the yield increased by about 1.4 times compared to Examples 41 to 57 in which no additional carbon dioxide was added. Further, in the case of q cyanobacteria (Example 244), the yield was increased about twice as compared with Example 57 in which no additional carbon dioxide was added.
- Production Example 5 Production of a solid-liquid mixture containing sugar (sugar cane)
- Sugar cane was purchased from a food store and washed. The liquid obtained by removing the sugar from the sugar cane juice was stirred to prepare 2000 g of molasses (a solid-liquid mixture containing sugar (sugar cane)).
- Production Example 6 Production of solid-liquid mixture containing sugar (yellow peach) Yellow peach was purchased from a food store and washed. Yellow peach was pulverized to prepare a solid-liquid mixture (yellow peach) containing sugar without adding 2000 g of water.
- Examples 245 to 254 and Comparative Example 17 660 g of the molasses produced in Production Example 5 (solid-liquid mixture containing sugar (sugar cane)) and 10 g of yeast and 870 g of water were stirred with a mixer, and the molasses containing sugar (solid-liquid mixture containing sugar (sugar cane)) 1540 was prepared.
- the prepared molasses containing yeast (solid-liquid mixture (sugar cane) containing sugar) was divided into 11 300 mL beakers of 140 g each, and 10 g of different plant pulverized products were added to each beaker by 5 g and stirred.
- Example 17 The solution was poured into a 300 mL syringe, a clear glass container (Examples 245 to 254).
- the 11 syringes prepared above were irradiated with light from a total of 600 light-emitting diodes composed of 200 white light-emitting diodes, 200 red light-emitting diodes and 200 blue light-emitting diodes (36 watts / hour in total) for 24 hours. did.
- the oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 93%.
- the solution in each syringe was distilled and the amount of alcohol recovered was measured. The above operation was performed three times in consideration of measurement errors. The results are summarized in Table 17.
- symbols a to j represent the following plants, and “area ratio” is the ratio (%) of the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectrum area recorded in the emission spectrum. Represents.
- Examples 255 to 264 and Comparative Example 18 A solid-liquid mixture (yellow peach) 1540 containing sugar containing yeast was prepared by stirring 660 g of the solid-liquid mixture (yellow peach) containing sugar produced in Production Example 6 and 10 g of yeast and 870 g of water with a mixer. The prepared solid-liquid mixture (yellow peach) containing sugar containing yeast is divided into 11 300 mL beakers of 140 g each, 10 g of different plant pulverized products are added to each beaker by 5 g and stirred, and then each solution is transparent. A 300 mL syringe, a glass container, was injected (Examples 255 to 264).
- the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 73%.
- the solution in each syringe was distilled and the amount of alcohol recovered was measured. The above operation was performed three times in consideration of measurement errors.
- the results are summarized in Table 18.
- symbols a to j represent the following plants, and “area ratio” is the ratio (%) of the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectrum area recorded in the emission spectrum. Represents.
- the ethanol production method of the present invention does not emit carbon dioxide, and thus is said to be a useful method in terms of CO 2 reduction. Moreover, since the manufacturing method of ethanol of this invention can obtain oxygen as a by-product, this can also be utilized as an effective resource. In addition, since the ethanol production method of the present invention can take in carbon dioxide from the outside and convert it into ethanol, it can also be a useful ethanol production method from the viewpoint of fixing carbon dioxide. .
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Abstract
Cette invention concerne un procédé de production d'éthanol facile à mettre en œuvre et dont le rendement de production est excellent. L'invention concerne un procédé de production d'éthanol comprenant : une étape d'irradiation d'un mélange constitué d'un mélange solide-liquide contenant du sucre, d'une cellule de levure et d'un chloroplaste avec une lumière émise par une diode électroluminescente dans un système fermé pour produire de l'éthanol ; et une étape de récupération de l'éthanol ainsi produit par le système à l'extérieur du système, les réactions suivantes a) à c) étant exécutées en même temps : a) réaction de fermentation du mélange solide-liquide contenant du sucre avec la cellule de levure pour produire de l'éthanol ; b) réaction de photosynthèse du dioxyde de carbone qui est produit lors de la fermentation a) avec le chloroplaste en irradiant avec une lumière émise par la diode électroluminescente pour produire un sucre ; et c) une réaction de fermentation du sucre produit dans la réaction b) avec la cellule de levure pour produire de l'éthanol.
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JP2008036674A JP4179399B1 (ja) | 2008-01-21 | 2008-01-21 | アルコールの製造方法 |
JP2008235164A JP4209462B1 (ja) | 2008-09-12 | 2008-09-12 | エタノールの製造方法 |
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JP2011254748A (ja) * | 2010-06-08 | 2011-12-22 | Kobe Univ | エタノールの生産方法 |
KR20170082519A (ko) * | 2014-10-01 | 2017-07-14 | 에그플랜트 에스.알.엘. | 바이오폴리머 매트릭스 복합체의 제조 방법 |
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US10465215B2 (en) | 2008-04-28 | 2019-11-05 | Naturally Scientific Technologies Limited | Production of biofuel from tissue culture sources |
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US10961387B2 (en) | 2014-10-01 | 2021-03-30 | Eggplant S.R.L. | Methods for producing biopolymer matrix composites |
KR102535278B1 (ko) | 2014-10-01 | 2023-05-22 | 에그플랜트 에스.알.엘. | 바이오폴리머 매트릭스 복합체의 제조 방법 |
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