WO2009091971A3 - Rapid chilling device for vitrification - Google Patents

Rapid chilling device for vitrification Download PDF

Info

Publication number
WO2009091971A3
WO2009091971A3 PCT/US2009/031242 US2009031242W WO2009091971A3 WO 2009091971 A3 WO2009091971 A3 WO 2009091971A3 US 2009031242 W US2009031242 W US 2009031242W WO 2009091971 A3 WO2009091971 A3 WO 2009091971A3
Authority
WO
WIPO (PCT)
Prior art keywords
cryogen
vitrification
chilling
self
liquid nitrogen
Prior art date
Application number
PCT/US2009/031242
Other languages
French (fr)
Other versions
WO2009091971A2 (en
Inventor
Milton Chin
Original Assignee
Vance Products Incorporated, D/B/A Cook Urological Incorporated
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vance Products Incorporated, D/B/A Cook Urological Incorporated filed Critical Vance Products Incorporated, D/B/A Cook Urological Incorporated
Priority to EP09702523A priority Critical patent/EP2229581A2/en
Priority to CN200980102441.XA priority patent/CN101939631A/en
Priority to JP2010543274A priority patent/JP2011511623A/en
Publication of WO2009091971A2 publication Critical patent/WO2009091971A2/en
Publication of WO2009091971A3 publication Critical patent/WO2009091971A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
    • A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
    • A01N1/0257—Stationary or portable vessels generating cryogenic temperatures
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0278—Physical preservation processes
    • A01N1/0289—Pressure processes, i.e. using a designated change in pressure over time

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Thermal Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Mechanical Engineering (AREA)
  • Chemical & Material Sciences (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Successful cryopreservation by the vitrification method depends on high chilling speed. Practitioners of vitrification prefer to use liquid nitrogen as the chilling cryogen due to its inherent safety and low cost. Plunging vitrification cryocontainers in to a quiescent pool of liquid nitrogen invariably results in a chilling rate less than the theoretical potential. The shortfall is attributed to the well-known Leidenfrost effect. The purpose of this invention it to provide improve chilling rates during vitrification using liquid nitrogen. One feature of this invention is a contacting device that invokes convective heat transfer principles to increase chilling speed. In another feature of this invention, cryogen velocity is derived from a self-pressurized dewar containing a saturated cryogen. The self-pressurization is achieved by ambient heating of the dewar's contents. In another embodiment, a sub-cooled cryogen, such as propane, is used in tandem with a saturated cryogen, such as LN2, in a self-pressurized dewar.
PCT/US2009/031242 2008-01-17 2009-01-16 Rapid chilling device for vitrification WO2009091971A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP09702523A EP2229581A2 (en) 2008-01-17 2009-01-16 Rapid chilling device for vitrification
CN200980102441.XA CN101939631A (en) 2008-01-17 2009-01-16 Be used for vitrified quick cooling device
JP2010543274A JP2011511623A (en) 2008-01-17 2009-01-16 Quenching equipment for vitrification

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US2166108P 2008-01-17 2008-01-17
US61/021,661 2008-01-17

Publications (2)

Publication Number Publication Date
WO2009091971A2 WO2009091971A2 (en) 2009-07-23
WO2009091971A3 true WO2009091971A3 (en) 2009-11-05

Family

ID=40673445

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/031242 WO2009091971A2 (en) 2008-01-17 2009-01-16 Rapid chilling device for vitrification

Country Status (5)

Country Link
US (1) US20090186405A1 (en)
EP (1) EP2229581A2 (en)
JP (1) JP2011511623A (en)
CN (1) CN101939631A (en)
WO (1) WO2009091971A2 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090120106A1 (en) * 2007-11-12 2009-05-14 Milton Chin Temperature Alert Device for Cryopreservation
US20090123996A1 (en) * 2007-11-12 2009-05-14 Milton Chin Vitrification Device with Shape Memory Seal
US9700038B2 (en) 2009-02-25 2017-07-11 Genea Limited Cryopreservation of biological cells and tissues
EP2575442B1 (en) 2010-05-28 2023-06-07 Genea Ip Holdings Pty Limited Improved micromanipulation and storage apparatus and methods
WO2013020032A2 (en) * 2011-08-04 2013-02-07 University Of Kansas Automated vitrification device
CN102423928B (en) * 2011-10-09 2014-06-04 中国科学院宁波材料技术与工程研究所 Composite film capable of reducing critical temperature of self-driven Leidenfrost liquid drops and preparation method for composite film
DE102011115467A1 (en) * 2011-10-10 2013-04-11 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Apparatus and method for pressure-cryopreserving a biological sample
EP2706342B1 (en) * 2012-09-11 2015-07-08 Fei Company Cooperating capillary and cap for use in a high-pressure freezer
US9297499B2 (en) 2012-12-06 2016-03-29 Cook Medical Technologies Llc Cryogenic storage container, storage device, and methods of using the same
US9518898B2 (en) 2012-12-06 2016-12-13 Cook Medical Technologies Llc Cryogenic storage container with sealing closure and methods of using the same
CN103706415B (en) * 2013-12-24 2015-06-17 华中科技大学 Sample rod for low-temperature storage of Dewar
US9723831B2 (en) 2014-07-30 2017-08-08 The Governing Council Of The University Of Toronto System and methods for automated vitrification of biological materials
RU2624963C1 (en) * 2016-04-18 2017-07-11 Целтроникс Device for super-quick cooling of biological samples up to cryogenic temperatures
CN106342789B (en) * 2016-10-13 2022-07-15 深圳韦拓生物科技有限公司 Cell vitrification freezing treatment equipment and treatment method thereof
EP3716761A1 (en) * 2017-11-28 2020-10-07 CooperSurgical, Inc. Specimen containers and related methods
EP3796779A4 (en) * 2018-05-22 2022-03-23 Mitegen, LLC System for rapid cooling and warming of cells and other biological material
NL2028903B1 (en) * 2021-08-02 2023-02-17 Marinus Van Den Berg Bartel Method and device for vitrifying biological material, such as semen
EP4166232A1 (en) * 2021-10-15 2023-04-19 Leica Mikrosysteme GmbH Storage container for storing a sample holder

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5024830A (en) * 1983-08-23 1991-06-18 The Board Of Regents, The University Of Texas Method for cryopreparing biological tissue for ultrastructural analysis
WO1994005995A1 (en) * 1992-09-08 1994-03-17 Leica Ag Device for dehydrating and/or embedding samples
EP0637741A1 (en) * 1993-08-03 1995-02-08 Martin Wohlwend Method and device for vitrifying samples, in particular biological samples
EP0853238A1 (en) * 1997-01-13 1998-07-15 Daniel Dr. Studer Sample holder for water-containing samples and method for use thereof
DE10015773A1 (en) * 2000-03-30 2001-10-04 Leica Mikrosysteme Gmbh Wien Sample holder used in high pressure freezer for preparing aqueous botanical sample, includes sample fastener with recess pressure-sealed, arranged in clearance of housing jacket
FR2891165A1 (en) * 2005-09-28 2007-03-30 Cryo Bio System Sa Conditioning unit for preserving biological substance by cryogenic vitrification, comprises an envelope with a thin tube, a support and a push rod
WO2007123720A2 (en) * 2006-03-30 2007-11-01 Cornell Research Foundation, Inc. System and method for increased cooling rates in rapid cooling of small biological samples

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US20090120106A1 (en) * 2007-11-12 2009-05-14 Milton Chin Temperature Alert Device for Cryopreservation

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5024830A (en) * 1983-08-23 1991-06-18 The Board Of Regents, The University Of Texas Method for cryopreparing biological tissue for ultrastructural analysis
WO1994005995A1 (en) * 1992-09-08 1994-03-17 Leica Ag Device for dehydrating and/or embedding samples
EP0637741A1 (en) * 1993-08-03 1995-02-08 Martin Wohlwend Method and device for vitrifying samples, in particular biological samples
EP0853238A1 (en) * 1997-01-13 1998-07-15 Daniel Dr. Studer Sample holder for water-containing samples and method for use thereof
DE10015773A1 (en) * 2000-03-30 2001-10-04 Leica Mikrosysteme Gmbh Wien Sample holder used in high pressure freezer for preparing aqueous botanical sample, includes sample fastener with recess pressure-sealed, arranged in clearance of housing jacket
FR2891165A1 (en) * 2005-09-28 2007-03-30 Cryo Bio System Sa Conditioning unit for preserving biological substance by cryogenic vitrification, comprises an envelope with a thin tube, a support and a push rod
WO2007123720A2 (en) * 2006-03-30 2007-11-01 Cornell Research Foundation, Inc. System and method for increased cooling rates in rapid cooling of small biological samples

Also Published As

Publication number Publication date
WO2009091971A2 (en) 2009-07-23
JP2011511623A (en) 2011-04-14
US20090186405A1 (en) 2009-07-23
EP2229581A2 (en) 2010-09-22
CN101939631A (en) 2011-01-05

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