WO2009069986A3 - Genetically-modified cell line for producing cloned miniature pigs for xenotransplantation and method for preparing the same. - Google Patents

Genetically-modified cell line for producing cloned miniature pigs for xenotransplantation and method for preparing the same. Download PDF

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Publication number
WO2009069986A3
WO2009069986A3 PCT/KR2008/007087 KR2008007087W WO2009069986A3 WO 2009069986 A3 WO2009069986 A3 WO 2009069986A3 KR 2008007087 W KR2008007087 W KR 2008007087W WO 2009069986 A3 WO2009069986 A3 WO 2009069986A3
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WO
WIPO (PCT)
Prior art keywords
xenotransplantation
genetically
preparing
cell line
same
Prior art date
Application number
PCT/KR2008/007087
Other languages
French (fr)
Other versions
WO2009069986A2 (en
Inventor
Kyung-Kwang Lee
Mira Chang
Jeong Woong Lee
Yong-Kook Kang
Man-Jong Kang
Jin-Hoi Kim
Hwan-Hoo Seong
Hosup Shim
Seongsoo Hwang
Original Assignee
Korea Res Inst Of Bioscience
Kyung-Kwang Lee
Mira Chang
Jeong Woong Lee
Yong-Kook Kang
Man-Jong Kang
Jin-Hoi Kim
Hwan-Hoo Seong
Hosup Shim
Seongsoo Hwang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Korea Res Inst Of Bioscience, Kyung-Kwang Lee, Mira Chang, Jeong Woong Lee, Yong-Kook Kang, Man-Jong Kang, Jin-Hoi Kim, Hwan-Hoo Seong, Hosup Shim, Seongsoo Hwang filed Critical Korea Res Inst Of Bioscience
Publication of WO2009069986A2 publication Critical patent/WO2009069986A2/en
Publication of WO2009069986A3 publication Critical patent/WO2009069986A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • A01K2267/025—Animal producing cells or organs for transplantation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Veterinary Medicine (AREA)
  • Environmental Sciences (AREA)
  • Cell Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to a targeting vector specific for a xenoantigen determining antigen synthesizing gene capable of knock-outing the xenoantigen determining antigen synthesizing gene using homologous recombination technologies and integrating human complement inhibiting gene and/or human CD39 gene at the loci of the xenoantigen determining antigen synthesizing gene.
PCT/KR2008/007087 2007-11-30 2008-12-01 Genetically-modified cell line for producing cloned miniature pigs for xenotransplantation and method for preparing the same. WO2009069986A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2007-0123823 2007-11-30
KR20070123823 2007-11-30

Publications (2)

Publication Number Publication Date
WO2009069986A2 WO2009069986A2 (en) 2009-06-04
WO2009069986A3 true WO2009069986A3 (en) 2009-07-16

Family

ID=40679163

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2008/007087 WO2009069986A2 (en) 2007-11-30 2008-12-01 Genetically-modified cell line for producing cloned miniature pigs for xenotransplantation and method for preparing the same.

Country Status (2)

Country Link
KR (1) KR101149475B1 (en)
WO (1) WO2009069986A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012504390A (en) * 2008-09-30 2012-02-23 ノボザイムス，インコーポレイティド Methods of using positive and negative selection genes in filamentous fungal cells
KR101048426B1 (en) * 2009-05-27 2011-07-11 한국생명공학연구원 Method for producing somatic cells obtained by adding the DAF gene to the position of the alpha 1,3-galactosyltransferase gene
KR101236724B1 (en) * 2010-11-23 2013-02-26 대한민국 Gene targeting knock-in vector containing overexpression cassette, the method for constructing the same and transgenic cloned animal carrying the vector for xenotransplantation
KR101896518B1 (en) * 2011-03-30 2018-10-24 주식회사 툴젠 Zinc finger nuclease for targeting CMAH gene and use thereof
KR101479671B1 (en) * 2011-11-02 2015-01-08 건국대학교 산학협력단 CMP-N-acetylneuraminic acid hydroxylase targeting vector and use of the same
US20160102319A1 (en) * 2013-04-30 2016-04-14 Konkuk University Industrial Cooperation Corp. Cmp-acetylneuraminic acid hydroxylase targeting vector, transgenic animal for xenotransplantation introduced with the vector, and method of manufacturing the same
WO2017044864A1 (en) * 2015-09-09 2017-03-16 Revivicor, Inc Multi-transgenic pig for xenotransplantation
KR102363891B1 (en) * 2017-04-28 2022-02-17 서울대학교산학협력단 Transgenic pigs expressing both shTNFRI-Fc and HA-HO-1 without Gal epitope and N-glycolylneuraminic acid epitope and the Use of thereof
CN113429475A (en) * 2020-03-23 2021-09-24 成都中科奥格生物科技有限公司 Glue raw material and preparation method and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6166288A (en) * 1995-09-27 2000-12-26 Nextran Inc. Method of producing transgenic animals for xenotransplantation expressing both an enzyme masking or reducing the level of the gal epitope and a complement inhibitor
US7166278B2 (en) * 2001-04-30 2007-01-23 Rbc Biotechnology, Inc. Modified organs and cells for xenotransplantation

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2233295A (en) 1994-04-13 1995-11-10 Biotransplant Incorporated Alpha(1,3) galactosyltransferase negative swine
JP2006129736A (en) 2004-11-02 2006-05-25 Nippon Dobutsu Kogaku Kenkyusho:Kk Pig cell for xenotransplantation, method for selecting the same, and pig for the xenotransplantation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6166288A (en) * 1995-09-27 2000-12-26 Nextran Inc. Method of producing transgenic animals for xenotransplantation expressing both an enzyme masking or reducing the level of the gal epitope and a complement inhibitor
US7166278B2 (en) * 2001-04-30 2007-01-23 Rbc Biotechnology, Inc. Modified organs and cells for xenotransplantation

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
COOPER, D. K. ET AL.: "Alpha 1,3-galactosyltransferase gene-knockout pigs for xenotransplantation: where do we go from here?", TRANSPLANTATION., vol. 84, no. 1, 15 July 2007 (2007-07-15), pages 1 - 7 *
COSTA, C. ET AL.: "Comparative analysis of three genetic modifications designed to inhibit human serum-mediated cytolysis.", XENOTRANSPLANTATION., vol. 6, no. 1, February 1999 (1999-02-01), pages 6 - 16 *
IKEYA, M. ET AL.: "Gene disruption/knock-in analysis of mONT3: vector construction by employing both in vivo and in vitro recombinations.", INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY., vol. 49, no. 7, 2005, pages 807 - 823 *

Also Published As

Publication number Publication date
WO2009069986A2 (en) 2009-06-04
KR101149475B1 (en) 2012-05-25
KR20090056922A (en) 2009-06-03

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