WO2009062241A8 - Crystal structure of a bacterial enzyme and uses thereof - Google Patents

Crystal structure of a bacterial enzyme and uses thereof Download PDF

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Publication number
WO2009062241A8
WO2009062241A8 PCT/AU2008/001679 AU2008001679W WO2009062241A8 WO 2009062241 A8 WO2009062241 A8 WO 2009062241A8 AU 2008001679 W AU2008001679 W AU 2008001679W WO 2009062241 A8 WO2009062241 A8 WO 2009062241A8
Authority
WO
WIPO (PCT)
Prior art keywords
crystal structure
staphylococcus aureus
protein ligase
antagonists
biotin protein
Prior art date
Application number
PCT/AU2008/001679
Other languages
French (fr)
Other versions
WO2009062241A1 (en
Inventor
Nicole Renee Pendini
John Wallace
Steven William Polyak
Grant William Booker
Andrew David Abell
Matthew Charles James Wilce
Original Assignee
Univ Monash
Adelaide Res & Innovation Pty
Nicole Renee Pendini
John Wallace
Steven William Polyak
Grant William Booker
Andrew David Abell
Matthew Charles James Wilce
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2007906228A external-priority patent/AU2007906228A0/en
Application filed by Univ Monash, Adelaide Res & Innovation Pty, Nicole Renee Pendini, John Wallace, Steven William Polyak, Grant William Booker, Andrew David Abell, Matthew Charles James Wilce filed Critical Univ Monash
Publication of WO2009062241A1 publication Critical patent/WO2009062241A1/en
Publication of WO2009062241A8 publication Critical patent/WO2009062241A8/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/9015—Ligases (6)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/20—Screening for compounds of potential therapeutic value cell-free systems

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Oncology (AREA)
  • General Engineering & Computer Science (AREA)
  • Hospice & Palliative Care (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention is predicated, in part, on the determination of the crystal structure of Staphylococcus aureus biotin protein ligase. The present invention provides, among other things, the crystal structure of Staphylococcus aureus biotin protein ligase complexed with biotinyl 5'-adenylate at a resolution of 2.6 Angstroms. Accordingly, the present invention also provides methods for the identification and/or rational design of antagonists and agonists of biotin protein ligase enzymes, and in particular, antagonists of Staphylococcus aureus biotin protein ligase. Such antagonists may be used to inhibit bacterial infections, including Staphylococcus aureus infections.
PCT/AU2008/001679 2007-11-13 2008-11-13 Crystal structure of a bacterial enzyme and uses thereof WO2009062241A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2007906228 2007-11-13
AU2007906228A AU2007906228A0 (en) 2007-11-13 Crystal structure of a bacterial enzyme and uses thereof

Publications (2)

Publication Number Publication Date
WO2009062241A1 WO2009062241A1 (en) 2009-05-22
WO2009062241A8 true WO2009062241A8 (en) 2009-07-30

Family

ID=40638239

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AU2008/001679 WO2009062241A1 (en) 2007-11-13 2008-11-13 Crystal structure of a bacterial enzyme and uses thereof

Country Status (1)

Country Link
WO (1) WO2009062241A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2758405A4 (en) * 2011-09-23 2015-04-29 Adelaide Res & Innovation Pty Novel antimicrobial compounds
WO2019143529A1 (en) * 2018-01-17 2019-07-25 The Board Of Trustees Of The Leland Stanford Junior University Engineered promiscuous biotin ligases for efficient proximity labeling

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6448038B1 (en) * 1999-01-26 2002-09-10 Smithkline Beecham Corporation BirA from staphylococcus aureus
AU2002328225A1 (en) * 2001-09-21 2003-04-01 Affinium Pharmaceuticals, Inc. Bacterial polypeptides involved in protein processing
EP1815006A4 (en) * 2004-11-23 2009-01-28 Adelaide Res & Innovation Pty Inhibitors of biotin protein ligase

Also Published As

Publication number Publication date
WO2009062241A1 (en) 2009-05-22

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