WO2009057313A1 - 内皮前駆細胞（ｅｐｃ）の増殖・分化誘導方法 - Google Patents

内皮前駆細胞（ｅｐｃ）の増殖・分化誘導方法 Download PDF

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Publication number
WO2009057313A1
WO2009057313A1 PCT/JP2008/003125 JP2008003125W WO2009057313A1 WO 2009057313 A1 WO2009057313 A1 WO 2009057313A1 JP 2008003125 W JP2008003125 W JP 2008003125W WO 2009057313 A1 WO2009057313 A1 WO 2009057313A1
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WO
WIPO (PCT)
Prior art keywords
epc
proliferation
differentiation
progenitor cell
endothelial progenitor
Prior art date
Application number
PCT/JP2008/003125
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English (en)
French (fr)
Inventor
Junichi Kawabe
Fumitaka Ushikubi
Original Assignee
National University Corporation Asahikawa Medical College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by National University Corporation Asahikawa Medical College filed Critical National University Corporation Asahikawa Medical College
Publication of WO2009057313A1 publication Critical patent/WO2009057313A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C12N5/0692—Stem cells; Progenitor cells; Precursor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/02—Compounds of the arachidonic acid pathway, e.g. prostaglandins, leukotrienes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Vascular Medicine (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Cell Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cardiology (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

内皮前駆細胞（ＥＰＣ）の増殖効率や内皮細胞への分化効率がより高く、より安全でより低コストな、ＥＰＣの増殖・分化方法を提供するものである。プロスタサイクリン又はプロスタサイクリンアナログ、及び、ヘパリン存在下で、内皮前駆細胞（ＥＰＣ）をインビトロ培養することにより、ＥＰＣの増殖効率を向上させると共に、内皮細胞への分化効率を向上させることが可能となった。この知見を利用することにより、上記課題を解決することができる。
PCT/JP2008/003125 2007-10-30 2008-10-30 内皮前駆細胞（ｅｐｃ）の増殖・分化誘導方法 WO2009057313A1 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-281161 2007-10-30
JP2007281161A JP2011015609A (ja) 2007-10-30 2007-10-30 内皮前駆細胞（ｅｐｃ）の増殖・分化誘導方法

Publications (1)

Publication Number Publication Date
WO2009057313A1 true WO2009057313A1 (ja) 2009-05-07

Family

ID=40590717

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/003125 WO2009057313A1 (ja) 2007-10-30 2008-10-30 内皮前駆細胞（ｅｐｃ）の増殖・分化誘導方法

Country Status (2)

Country Link
JP (1) JP2011015609A (ja)
WO (1) WO2009057313A1 (ja)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014022376A3 (en) * 2012-08-01 2015-07-16 United Therapeutics Corporation Treatment of pulmonary arterial hypertension with prostacyclin-treated endothelial progenitor cells
EP2879682B1 (en) * 2012-08-01 2018-03-21 United Therapeutics Corporation Treatment of pulmonary arterial hypertension with mesenchymal stem cells
EP2943069B1 (en) * 2013-01-09 2018-08-15 United Therapeutics Corporation Treatment of vasculopathy with prostacyclin and mesenchymal stem cells
CN111053788A (zh) * 2019-12-25 2020-04-24 广州市天河诺亚生物工程有限公司 一种用于慢性出血辅助治疗的组合物及其应用
US11571444B2 (en) 2016-10-24 2023-02-07 United Therapeutics Corporation Enhancement of MSC immunomodulatory properties by treprostinil

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006090886A1 (ja) * 2005-02-23 2006-08-31 Foundation For Biomedical Research And Innovation 血管内皮前駆細胞分化動態解析方法
WO2006090882A1 (ja) * 2005-02-23 2006-08-31 Foundation For Biomedical Research And Innovation 血管内皮前駆細胞の生体外増幅方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006090886A1 (ja) * 2005-02-23 2006-08-31 Foundation For Biomedical Research And Innovation 血管内皮前駆細胞分化動態解析方法
WO2006090882A1 (ja) * 2005-02-23 2006-08-31 Foundation For Biomedical Research And Innovation 血管内皮前駆細胞の生体外増幅方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KAWABE J. ET AL.: "Role of autocrine prostacyclin system in crucial functions of endothelial progenitor cells.", CIRC. J., vol. 72, no. SUPPL., 1 March 2008 (2008-03-01), pages 503 - PE.570 *
KAWABE J. ET AL.: "Role of prostacyclin in differentiation of endothelial progenitor cells and vascular remodeling.", J. PHARMACOL. SCI., vol. 103, no. SUPPL., 28 February 2007 (2007-02-28), pages 245 - P3.093 *
TAKAAKI SASAKI ET AL.: "Kotsuzui Yurai Kekkan Naihi Zenku Saibo no Seigyo ni yoru Shuyo Zoshoku no Yokusei - Shosaibo Haigan no Chiryo eno Oyo", JAPANESE JOURNAL OF LUNG CANCER, vol. 47, no. 5, 15 October 2007 (2007-10-15), pages 464 - WS9.1 *

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10842823B2 (en) 2012-08-01 2020-11-24 United Therapeutics Corporation Treatment of pulmonary arterial hypertension with prostacyclin-treated endothelial progenitor cells
JP2015524438A (ja) * 2012-08-01 2015-08-24 ユナイテッド セラピューティクス コーポレイション プロスタサイクリン処理された内皮前駆細胞による肺動脈性高血圧症の処置
CN104936603A (zh) * 2012-08-01 2015-09-23 联合治疗公司 利用前列环素处理的内皮祖细胞的肺动脉高血压的治疗
EP2879682B1 (en) * 2012-08-01 2018-03-21 United Therapeutics Corporation Treatment of pulmonary arterial hypertension with mesenchymal stem cells
US10016463B2 (en) 2012-08-01 2018-07-10 United Therapeutics Corporation Treatment of pulmonary arterial hypertension with prostacyclin-treated endothelial progenitor cells
US11839631B2 (en) 2012-08-01 2023-12-12 United Therapeutics Corporation Treatment of pulmonary arterial hypertension with prostacyclin-treated endothelial progenitor cells
US10071123B2 (en) 2012-08-01 2018-09-11 United Therapeutics Corporation Treatment of pulmonary arterial hypertension with mesenchymal stem cells
US11666602B2 (en) 2012-08-01 2023-06-06 United Therapeutics Corporation Treatment of pulmonary arterial hypertension with mesenchymal stem cells
WO2014022376A3 (en) * 2012-08-01 2015-07-16 United Therapeutics Corporation Treatment of pulmonary arterial hypertension with prostacyclin-treated endothelial progenitor cells
EP3492084A1 (en) * 2012-08-01 2019-06-05 United Therapeutics Corporation Treatment of pulmonary arterial hypertension with prostacyclin-treated endothelial progenitor cells
EP3415150A1 (en) * 2013-01-09 2018-12-19 United Therapeutics Corporation Treatment of vasculopathy with prostacyclin and mesenchymal stem cells
US11141393B2 (en) 2013-01-09 2021-10-12 United Therapeutics Corporation Treatment of vasculopathy with prostacyclin and mesenchymal stem cells
US10080730B2 (en) 2013-01-09 2018-09-25 United Therapeutics Corporation Treatment of vasculopathy with prostacyclin and mesenchymal stem cells
EP2943069B1 (en) * 2013-01-09 2018-08-15 United Therapeutics Corporation Treatment of vasculopathy with prostacyclin and mesenchymal stem cells
US11839596B2 (en) 2013-01-09 2023-12-12 United Therapeutics Corporation Treatment of vasculopathy with prostacyclin and mesenchymal stem cells
US11571444B2 (en) 2016-10-24 2023-02-07 United Therapeutics Corporation Enhancement of MSC immunomodulatory properties by treprostinil
CN111053788A (zh) * 2019-12-25 2020-04-24 广州市天河诺亚生物工程有限公司 一种用于慢性出血辅助治疗的组合物及其应用

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JP2011015609A (ja) 2011-01-27

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