WO2009055656A3 - Vecteurs, cellules hôtes, procédés de production et utilisations - Google Patents
Vecteurs, cellules hôtes, procédés de production et utilisations Download PDFInfo
- Publication number
- WO2009055656A3 WO2009055656A3 PCT/US2008/081090 US2008081090W WO2009055656A3 WO 2009055656 A3 WO2009055656 A3 WO 2009055656A3 US 2008081090 W US2008081090 W US 2008081090W WO 2009055656 A3 WO2009055656 A3 WO 2009055656A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- vectors
- antibody
- plasmids
- incorporate
- production
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Veterinary Medicine (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Les vecteurs d'expression d'anticorps et les plasmides de l'invention peuvent incorporer diverses parties de gènes d'anticorps pour la transcription de l'ADN de l'anticorps et l'expression de l'anticorps dans une cellule hôte appropriée. Lesdits vecteurs d'expression et plasmides ont des sites de restriction qui facilitent la ligature de l'ADN codant pour l'anticorps dans les vecteurs. Les vecteurs comprennent des séquences d'activateur et de promoteur que l'on peut faire varier pour interagir avec les facteurs de transcription dans la cellule hôte et diriger ainsi la transcription de l'ADN codant pour l'anticorps. L'invention concerne également une trousse pouvant contenir ces vecteurs et plasmides.
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12/738,453 US8663980B2 (en) | 2007-10-26 | 2008-10-24 | Vectors, host cells, and methods of production and uses |
EP08842483A EP2214708A4 (fr) | 2007-10-26 | 2008-10-24 | Vecteurs, cellules hôtes, procédés de production et utilisations |
US14/163,469 US8980626B2 (en) | 2007-10-26 | 2014-01-24 | Vectors, host cells, and methods of production and uses |
US14/617,003 US9206248B2 (en) | 2007-10-26 | 2015-02-09 | Vectors, host cells, and methods of production and uses |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US98281407P | 2007-10-26 | 2007-10-26 | |
US60/982,814 | 2007-10-26 |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US12/738,453 A-371-Of-International US8663980B2 (en) | 2007-10-26 | 2008-10-24 | Vectors, host cells, and methods of production and uses |
US14/163,469 Division US8980626B2 (en) | 2007-10-26 | 2014-01-24 | Vectors, host cells, and methods of production and uses |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2009055656A2 WO2009055656A2 (fr) | 2009-04-30 |
WO2009055656A3 true WO2009055656A3 (fr) | 2009-12-30 |
Family
ID=40580404
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2008/081090 WO2009055656A2 (fr) | 2007-10-26 | 2008-10-24 | Vecteurs, cellules hôtes, procédés de production et utilisations |
Country Status (3)
Country | Link |
---|---|
US (3) | US8663980B2 (fr) |
EP (1) | EP2214708A4 (fr) |
WO (1) | WO2009055656A2 (fr) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2214708A4 (fr) * | 2007-10-26 | 2011-01-12 | Centocor Ortho Biotech Inc | Vecteurs, cellules hôtes, procédés de production et utilisations |
EP2938726B1 (fr) | 2012-12-31 | 2017-09-27 | Boehringer Ingelheim International GmbH | Intron hétérologue dans un peptide signal |
WO2014102101A1 (fr) * | 2012-12-31 | 2014-07-03 | Boehringer Ingelheim International Gmbh | Séquences de nouveaux introns |
WO2014102104A1 (fr) | 2012-12-31 | 2014-07-03 | Boehringer Ingelheim International Gmbh | Introns artificiels |
WO2014102100A1 (fr) | 2012-12-31 | 2014-07-03 | Boehringer Ingelheim International Gmbh | Intron hétérologue dans un domaine d'immunoglobuline |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020055173A1 (en) * | 1997-11-25 | 2002-05-09 | Parks Christopher L. | Method for preparing adenovirus vectors, vectors so prepared, and uses thereof |
US20070107075A1 (en) * | 2001-03-29 | 2007-05-10 | Klein Robert D | Disruptions in GABA Receptor RHO2 Subunit, Methods and Uses Thereof |
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US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
DE3587814T2 (de) | 1985-03-30 | 1994-11-10 | Marc Ballivet | Verfahren zum erhalten von dns, rns, peptiden, polypeptiden oder proteinen durch dns-rekombinant-verfahren. |
US5618920A (en) | 1985-11-01 | 1997-04-08 | Xoma Corporation | Modular assembly of antibody genes, antibodies prepared thereby and use |
DE3600905A1 (de) | 1986-01-15 | 1987-07-16 | Ant Nachrichtentech | Verfahren zum dekodieren von binaersignalen sowie viterbi-dekoder und anwendungen |
US5225539A (en) | 1986-03-27 | 1993-07-06 | Medical Research Council | Recombinant altered antibodies and methods of making altered antibodies |
ATE141646T1 (de) | 1986-04-09 | 1996-09-15 | Genzyme Corp | Genetisch transformierte tiere, die ein gewünschtes protein in milch absondern |
US5763192A (en) | 1986-11-20 | 1998-06-09 | Ixsys, Incorporated | Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique |
EP0279582A3 (fr) | 1987-02-17 | 1989-10-18 | Pharming B.V. | Séquences d'ADN pour diriger des protéines vers les glandes mammaires afin d'être sécrétées efficacement |
US4873316A (en) | 1987-06-23 | 1989-10-10 | Biogen, Inc. | Isolation of exogenous recombinant proteins from the milk of transgenic mammals |
GB8826530D0 (en) | 1988-11-12 | 1988-12-14 | Ped Capacitors Ltd | Electrical capacitors |
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FR2646438B1 (fr) | 1989-03-20 | 2007-11-02 | Pasteur Institut | Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration |
CA2057923A1 (fr) | 1989-05-16 | 1990-11-17 | William D. Huse | Co-expression de recepteurs heteromeriques |
CA2016841C (fr) | 1989-05-16 | 1999-09-21 | William D. Huse | Methode de production de polymeres ayant une activite choisie |
CA2016842A1 (fr) | 1989-05-16 | 1990-11-16 | Richard A. Lerner | Methode pour puiser dans le repertoire immunologique |
EP0950707B1 (fr) | 1989-07-25 | 2009-02-18 | Cell Genesys, Inc. | Recombinaison homologue pour cellules donneuses universelles et hôtes mammifères chimériques |
CA2086741A1 (fr) | 1990-07-10 | 1992-01-11 | Paul E. Bender | Oxamides |
GB9015198D0 (en) | 1990-07-10 | 1990-08-29 | Brien Caroline J O | Binding substance |
DE69130647T2 (de) | 1990-08-24 | 1999-05-06 | Ixsys, Inc., San Diego, Calif. | Verfahren zur herstellung von oligonukleotiden mit regellosen codonen |
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KR100272077B1 (ko) | 1990-08-29 | 2000-11-15 | 젠팜인터내셔날,인코포레이티드 | 이종 항체를 생산할 수 있는 전이유전자를 가진 인간이외의 동물 |
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WO1994018219A1 (fr) | 1993-02-02 | 1994-08-18 | The Scripps Research Institute | Procedes de production de banques d'anticorps utilisant des chaines legeres d'immunoglobulines universelles ou aleatoires |
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ATE505552T1 (de) | 2005-10-21 | 2011-04-15 | Hoffmann La Roche | Methode zur rekombinanten expression eines polyeptids |
EP2214708A4 (fr) * | 2007-10-26 | 2011-01-12 | Centocor Ortho Biotech Inc | Vecteurs, cellules hôtes, procédés de production et utilisations |
-
2008
- 2008-10-24 EP EP08842483A patent/EP2214708A4/fr not_active Withdrawn
- 2008-10-24 US US12/738,453 patent/US8663980B2/en active Active
- 2008-10-24 WO PCT/US2008/081090 patent/WO2009055656A2/fr active Application Filing
-
2014
- 2014-01-24 US US14/163,469 patent/US8980626B2/en active Active
-
2015
- 2015-02-09 US US14/617,003 patent/US9206248B2/en active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020055173A1 (en) * | 1997-11-25 | 2002-05-09 | Parks Christopher L. | Method for preparing adenovirus vectors, vectors so prepared, and uses thereof |
US20070107075A1 (en) * | 2001-03-29 | 2007-05-10 | Klein Robert D | Disruptions in GABA Receptor RHO2 Subunit, Methods and Uses Thereof |
Non-Patent Citations (1)
Title |
---|
See also references of EP2214708A4 * |
Also Published As
Publication number | Publication date |
---|---|
US20110008321A1 (en) | 2011-01-13 |
WO2009055656A2 (fr) | 2009-04-30 |
US8980626B2 (en) | 2015-03-17 |
EP2214708A4 (fr) | 2011-01-12 |
EP2214708A2 (fr) | 2010-08-11 |
US9206248B2 (en) | 2015-12-08 |
US20150147805A1 (en) | 2015-05-28 |
US8663980B2 (en) | 2014-03-04 |
US20140141506A1 (en) | 2014-05-22 |
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