WO2009055366A1 - Procédé et kit utilisés pour réaliser un profilage génomique à haute résolution de populations de cellules clonales dans des tissus hétérogènes - Google Patents

Procédé et kit utilisés pour réaliser un profilage génomique à haute résolution de populations de cellules clonales dans des tissus hétérogènes Download PDF

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Publication number
WO2009055366A1
WO2009055366A1 PCT/US2008/080606 US2008080606W WO2009055366A1 WO 2009055366 A1 WO2009055366 A1 WO 2009055366A1 US 2008080606 W US2008080606 W US 2008080606W WO 2009055366 A1 WO2009055366 A1 WO 2009055366A1
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WO
WIPO (PCT)
Prior art keywords
dna
cell
interest
cells
copy number
Prior art date
Application number
PCT/US2008/080606
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English (en)
Inventor
Michael Barrett
Original Assignee
Translational Genomics Research Institute (Tgen)
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Publication date
Application filed by Translational Genomics Research Institute (Tgen) filed Critical Translational Genomics Research Institute (Tgen)
Publication of WO2009055366A1 publication Critical patent/WO2009055366A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention porte sur des procédés d'évaluation de l'aberration du nombre de copies dans une population clonale de cellules. Le procédé comprend les opérations consistant à purifier des noyaux provenant de cellules d'intérêt à l'aide d'un tri de cellules activées par fluorescence, amplifier l'ADN provenant des noyaux avec une ADN polymérase hautement active telle que phi29, traiter l'ADN avec une enzyme qui catalyse des ruptures dans l'ADN telle que l'ADNase ou une endonucléase de restriction, marquer l'ADN par un marqueur fluorescent et hybrider l'ADN à une puce à ADN capable d'effectuer une hybridation génomique comparative sur microréseau d'ADN. Les cellules d'intérêt comprennent des cellules tumorales et peuvent être issues de tissu noyé dans la paraffine fixé par du formaldéhyde. Les paramètres pour isoler les noyaux comprennent la teneur en ADN et l'état de cycle cellulaire et les noyaux peuvent être isolés par centrifugation ou autre dissociation mécanique en présence ou en l'absence de marqueur fluorescent. Un ADN témoin peut être inclus et marqué par un marqueur fluorescent autre que celui de la molécule d'ADN provenant de la cellule d'intérêt.
PCT/US2008/080606 2007-10-22 2008-10-21 Procédé et kit utilisés pour réaliser un profilage génomique à haute résolution de populations de cellules clonales dans des tissus hétérogènes WO2009055366A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US98181107P 2007-10-22 2007-10-22
US60/981,811 2007-10-22

Publications (1)

Publication Number Publication Date
WO2009055366A1 true WO2009055366A1 (fr) 2009-04-30

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/080606 WO2009055366A1 (fr) 2007-10-22 2008-10-21 Procédé et kit utilisés pour réaliser un profilage génomique à haute résolution de populations de cellules clonales dans des tissus hétérogènes

Country Status (1)

Country Link
WO (1) WO2009055366A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102686747A (zh) * 2009-11-23 2012-09-19 贝克顿·迪金森公司 使用探针杂交和限制性通过信号扩大而检测靶核酸的方法
WO2013123463A1 (fr) * 2012-02-15 2013-08-22 The Translational Genomics Research Institute Système et procédé de profilage génomique
CN104316373A (zh) * 2014-10-22 2015-01-28 江苏省农业科学院 一种适于流式细胞分析的茄子叶片细胞核提取方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040241658A1 (en) * 2003-05-28 2004-12-02 Barrett Michael T. Comparative genomic hybridiztion assays using immobilized oligonucleotide targets with initially small sample sizes and compositions for practicing the same
US20070105886A1 (en) * 2001-10-18 2007-05-10 Potter Barry Victor L Steroidal compounds for inhibiting steroid sulphatase

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070105886A1 (en) * 2001-10-18 2007-05-10 Potter Barry Victor L Steroidal compounds for inhibiting steroid sulphatase
US20040241658A1 (en) * 2003-05-28 2004-12-02 Barrett Michael T. Comparative genomic hybridiztion assays using immobilized oligonucleotide targets with initially small sample sizes and compositions for practicing the same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BOIGE ET AL.: "Concerted nonsyntenic allelic losses in hyperploid hepatocellular carcinoma as determined by a high-resolution allelotype.", CANCER RESEARCH, vol. 57, 1997, pages 1986 - 1990 *
BOROZDIN ET AL.: "Multigene deletions on chromosome 20q13.13-q13.2 including SALL4 result in an expanded phenotype of Okihiro syndrome plus developmental delay.", HUMAN MUTATION, vol. 28, August 2007 (2007-08-01), pages 830 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102686747A (zh) * 2009-11-23 2012-09-19 贝克顿·迪金森公司 使用探针杂交和限制性通过信号扩大而检测靶核酸的方法
US8916349B2 (en) 2009-11-23 2014-12-23 Becton, Dickinson And Company Assay method for target nucleic acid by signal amplification using probe hybridization and restriction
WO2013123463A1 (fr) * 2012-02-15 2013-08-22 The Translational Genomics Research Institute Système et procédé de profilage génomique
JP2015508657A (ja) * 2012-02-15 2015-03-23 ザ・トランスレーショナル・ジェノミクス・リサーチ・インスティチュート ゲノムプロファイリングのシステムおよび方法
EP2814987A4 (fr) * 2012-02-15 2015-10-14 Translational Genomics Res Inst Système et procédé de profilage génomique
US9663826B2 (en) 2012-02-15 2017-05-30 The Translational Genomics Research Institute System and method of genomic profiling
CN104316373A (zh) * 2014-10-22 2015-01-28 江苏省农业科学院 一种适于流式细胞分析的茄子叶片细胞核提取方法

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