WO2009050277A2 - Edta resistant s100a12 complexes (erac) - Google Patents
Edta resistant s100a12 complexes (erac) Download PDFInfo
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- WO2009050277A2 WO2009050277A2 PCT/EP2008/064058 EP2008064058W WO2009050277A2 WO 2009050277 A2 WO2009050277 A2 WO 2009050277A2 EP 2008064058 W EP2008064058 W EP 2008064058W WO 2009050277 A2 WO2009050277 A2 WO 2009050277A2
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- erac
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Definitions
- a method for detection of ERAC comprising the steps of i) providing a sample, ii) removing divalent metal ions from said sample, thereby reducing or eliminating the amount of free divalent metal ion in the sample, iii) providing a labelled first targeting species capable of directly detecting
- said other immunological marker is calprotectin.
- a method for treatment of a clinical condition comprising the step of i) reducing in an individual in need thereof the amount of ERAC present in a body fluid of said individual.
- the present invention is also directed to variant polypeptides having one or more conservative amino acid substitution(s) and polynucleotides encoding polypeptides having one or more conservative amino acid substitution(s), as compared with the amino acid sequence of SEQ ID NO:1 .
- Variants of SEQ ID NO:1 include sequences wherein e.g.
- the immunogenicity of a polypeptide can be increased through the use of an adjuvant, such as alum (aluminum hydroxide) or Freund's complete or incomplete adjuvant.
- an adjuvant such as alum (aluminum hydroxide) or Freund's complete or incomplete adjuvant.
- Polypeptides useful for immunization also include fusion polypeptides, such as fusions of ERAC, or a portion thereof, with an immunoglobulin polypeptide, or with maltose binding protein.
- the polypeptide immunogen may be a full-length molecule or a portion thereof.
- Possible pharmaceuticals in this respect could be anti-inflammatory medicines e.g. acetyl-salicylic acid and statins.
- Another mechanism, by which pharmaceuticals could interfere in a hypothesised pathological binding to receptors could be RAGE antagonists, analogue to the mechanisms of TNF- ⁇ antagonist.
- the present invention provides in an embodiment a method for detection of ERAC in a specimen or sample, wherein said specimen, optionally treated to remove undesired components, is contacted with a kit comprising a targeting species, preferably an antibody, directed against ERAC.
- the contacting results in the case of an antibody being used in the formation of immuno-complexes with ERAC antigens.
- the micro flow system enables immobilization of reactant streams, in the present context streams of labelled targeting species and subsequent testing with one or several samples creating a weave with thousands of intersection points where chemical reactions occur and are detected.
- the entire procedure is performed in a closed fluidic system providing the flexibility in terms of sample - and reactant application, choice of immobilization - and detection chemistries, and array layout.
- the kit for testing for a plurality of immunological markers such as providing profiles of immunological markers or a profile of autoantibodies
- the invention suitably includes the use of the kit in a microsystem.
- the invention employs the detection of the primary antibody by immunochemical reaction with specific so-called secondary antibodies capable of reacting with the primary antibodies.
- the secondary antibodies are preferably labelled with an appropriate label such as an enzyme, an isotope, a fluorescent group or a heavy metal such as gold.
- the visual detection is based on a cut-off point above which one colour indicates the presence of the ERAC above a certain minimum amount (cut-off point), and below which cut-off point another colour indicates that the ERAC is present in an amount of less than that indicated by the cut-off point.
- cut-off point a certain minimum amount
- another colour indicates that the ERAC is present in an amount of less than that indicated by the cut-off point.
- Example 1 Chromatographic and enzyme immunoassays for assessment of ERAC
- a serum sample is run on a gel permeation chromatography, for instance a 1.6 x 60 cm column of the type High-Load Superdex-75 from Pharmacia, Sweden, using a buffer consisting of 50 mM tris, 150 mM sodium chloride and 5 mM ETTA, pH 8.0. A flow rate of 1 ml/min is chosen. Proteins with high molecular weights are eluted earlier than those with low molecular weights. ERAC and A12 in fractions eluted from the column are assessed by the ELISA described below.
- anti-ERAC antibodies will bind to the walls of the wells.
- the wells must be covered by use of adhesive tape. Before use, the wells must be washed four times, each time with 250 microliters per well, with PBS containing 0.5 ml Tween- 20 per liter.
- GPC Gel Permeation Chromatography
- the labelled antibodies against S100A12 will bind to any S100A12 molecules or complexes containing this protein; when such antibody-antigen complexes reach the Test zone they will be bound by the antibodies there and give it a colour like that of the label on the antibodies.
- the colour intensity will increase will increase with time and reach a maximum after about one hour. For quantitative assay, an incubation period of about 10 minutes may be preferred to have a quick result as expected from a rapid test.
- a similarly coloured Control zone will appear in the test strip in a position corresponding to the zone where Control antibody had been applied. This zone serves the function of confirming that a sample has been applied, that the dissolution of dried protein occurred and that the diffusion into the NC membrane took place. For quantitative purposes it is possible to use the ratio of staining intensities of Test zone and Control zone to compensate for possible variability in the sample pad or NC membrane.
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
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- Bioinformatics & Cheminformatics (AREA)
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- Hematology (AREA)
- Molecular Biology (AREA)
- Communicable Diseases (AREA)
- Cardiology (AREA)
- Rheumatology (AREA)
- Biomedical Technology (AREA)
- Pulmonology (AREA)
- Heart & Thoracic Surgery (AREA)
- Physical Education & Sports Medicine (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Biotechnology (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Biochemistry (AREA)
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Abstract
Description
Claims
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12/738,579 US20110312922A1 (en) | 2007-10-19 | 2008-10-17 | EDTA Resistant S100A12 Complexes (ERAC) |
JP2010529401A JP2011502244A (en) | 2007-10-19 | 2008-10-17 | EDTA resistant S100A12 complex (ERAC) |
AU2008313688A AU2008313688A1 (en) | 2007-10-19 | 2008-10-17 | EDTA resistant S100A12 complexes (ERAC) |
CA2702533A CA2702533A1 (en) | 2007-10-19 | 2008-10-17 | Edta resistant s100a12 complexes (erac) |
EP08840332A EP2210102A2 (en) | 2007-10-19 | 2008-10-17 | Edta resistant s100a12 complexes (erac) |
CN2008801122278A CN101903775A (en) | 2007-10-19 | 2008-10-17 | The S100A12C compound (ERAC) of anti-EDTA |
IL205039A IL205039A0 (en) | 2007-10-19 | 2010-04-13 | Edta resistant s100a12 complexes (erac) |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US99965307P | 2007-10-19 | 2007-10-19 | |
DKPA200701511 | 2007-10-19 | ||
DKPA200701511 | 2007-10-19 | ||
US60/999,653 | 2007-10-19 |
Publications (2)
Publication Number | Publication Date |
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WO2009050277A2 true WO2009050277A2 (en) | 2009-04-23 |
WO2009050277A3 WO2009050277A3 (en) | 2009-06-25 |
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Application Number | Title | Priority Date | Filing Date |
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PCT/EP2008/064058 WO2009050277A2 (en) | 2007-10-19 | 2008-10-17 | Edta resistant s100a12 complexes (erac) |
Country Status (9)
Country | Link |
---|---|
US (1) | US20110312922A1 (en) |
EP (1) | EP2210102A2 (en) |
JP (1) | JP2011502244A (en) |
CN (1) | CN101903775A (en) |
AU (1) | AU2008313688A1 (en) |
CA (1) | CA2702533A1 (en) |
IL (1) | IL205039A0 (en) |
RU (1) | RU2010119950A (en) |
WO (1) | WO2009050277A2 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8546440B2 (en) | 2008-12-18 | 2013-10-01 | Janssen Pharmaceuticals, Inc. | Substituted bicyclic imidazole derivatives as gamma secretase modulators |
WO2013167727A3 (en) * | 2012-05-11 | 2014-01-09 | Westfaelische Wilhelms-Universitaet Muenster | Method for determining arthritis relapse risk |
US8835482B2 (en) | 2009-05-07 | 2014-09-16 | Janssen Pharmaceuticals, Inc. | Substituted indazole and aza-indazole derivatives as gamma secretase modulators |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102706971A (en) * | 2010-12-09 | 2012-10-03 | 江南大学 | A method for analysis of corticosteroids in cosmetics utilizing reverse microemulsion electrokinetic chromatography by taking an ionic liquid as an additive |
JP5984795B2 (en) * | 2011-04-05 | 2016-09-06 | オリンパス株式会社 | How to collect data to detect pancreatic disease |
CN107576785A (en) * | 2017-08-25 | 2018-01-12 | 广州市雷德生物科技有限公司 | A kind of sample treatment solution and its application |
US11905561B2 (en) | 2018-10-16 | 2024-02-20 | King Faisal Specialist Hospital & Research Centre | Method for diagnosing or treating pulmonary fibrosis using S100A13 protein |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0731166A2 (en) * | 1995-03-06 | 1996-09-11 | Tonen Corporation | Novel calcium-binding proteins |
WO2000020621A1 (en) * | 1998-10-06 | 2000-04-13 | The Trustees Of Columbia University In The City Of New York | Extracellular novel rage binding protein (en-rage) and uses thereof |
WO2003069341A2 (en) * | 2002-02-15 | 2003-08-21 | Clemens Sorg | Method of diagnosis of inflammatory diseases using calgranulin c |
US20030224386A1 (en) * | 2001-12-19 | 2003-12-04 | Millennium Pharmaceuticals, Inc. | Compositions, kits, and methods for identification, assessment, prevention, and therapy of rheumatoid arthritis |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6790443B2 (en) * | 1996-11-22 | 2004-09-14 | The Trustees Of Columbia University In The City Of New York | Method for treating symptoms of diabetes |
US20070148704A1 (en) * | 2005-10-06 | 2007-06-28 | Ursula Klause | Anti-CCPand antinuclear antibodies in diagnosis of rheumatoid arthritis |
-
2008
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- 2008-10-17 EP EP08840332A patent/EP2210102A2/en not_active Withdrawn
- 2008-10-17 US US12/738,579 patent/US20110312922A1/en not_active Abandoned
- 2008-10-17 WO PCT/EP2008/064058 patent/WO2009050277A2/en active Application Filing
- 2008-10-17 AU AU2008313688A patent/AU2008313688A1/en not_active Abandoned
- 2008-10-17 JP JP2010529401A patent/JP2011502244A/en active Pending
- 2008-10-17 RU RU2010119950/15A patent/RU2010119950A/en not_active Application Discontinuation
- 2008-10-17 CA CA2702533A patent/CA2702533A1/en not_active Abandoned
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2010
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WO2000020621A1 (en) * | 1998-10-06 | 2000-04-13 | The Trustees Of Columbia University In The City Of New York | Extracellular novel rage binding protein (en-rage) and uses thereof |
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LARSEN A ET AL: "Quantification of S100A12 (EN-RAGE) in blood varies with sampling method, calcium and heparin" SCANDINAVIAN JOURNAL OF IMMUNOLOGY, vol. 65, no. 2, February 2007 (2007-02), pages 192-201, XP002514755 ISSN: 0300-9475 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8546440B2 (en) | 2008-12-18 | 2013-10-01 | Janssen Pharmaceuticals, Inc. | Substituted bicyclic imidazole derivatives as gamma secretase modulators |
US8835482B2 (en) | 2009-05-07 | 2014-09-16 | Janssen Pharmaceuticals, Inc. | Substituted indazole and aza-indazole derivatives as gamma secretase modulators |
WO2013167727A3 (en) * | 2012-05-11 | 2014-01-09 | Westfaelische Wilhelms-Universitaet Muenster | Method for determining arthritis relapse risk |
Also Published As
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EP2210102A2 (en) | 2010-07-28 |
CA2702533A1 (en) | 2009-04-23 |
WO2009050277A3 (en) | 2009-06-25 |
AU2008313688A1 (en) | 2009-04-23 |
RU2010119950A (en) | 2011-11-27 |
JP2011502244A (en) | 2011-01-20 |
IL205039A0 (en) | 2010-11-30 |
US20110312922A1 (en) | 2011-12-22 |
CN101903775A (en) | 2010-12-01 |
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