WO2009047189A3 - Fluoreszenzlichtmikroskopisches messen einer probe mit rotverschobenen stokes-linien - Google Patents

Fluoreszenzlichtmikroskopisches messen einer probe mit rotverschobenen stokes-linien Download PDF

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Publication number
WO2009047189A3
WO2009047189A3 PCT/EP2008/063171 EP2008063171W WO2009047189A3 WO 2009047189 A3 WO2009047189 A3 WO 2009047189A3 EP 2008063171 W EP2008063171 W EP 2008063171W WO 2009047189 A3 WO2009047189 A3 WO 2009047189A3
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WO
WIPO (PCT)
Prior art keywords
sample
red
light
fluorescent light
wavelength
Prior art date
Application number
PCT/EP2008/063171
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English (en)
French (fr)
Other versions
WO2009047189A2 (de
Inventor
Stefan W. Hell
Brian Rankin
Robert Kellner
Jaydev Jethwa
Thorsten Staudt
Original Assignee
Max-Planck-Gesellschaft zur Förderung der Wissenschaften e. V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max-Planck-Gesellschaft zur Förderung der Wissenschaften e. V. filed Critical Max-Planck-Gesellschaft zur Förderung der Wissenschaften e. V.
Priority to EP08837241A priority Critical patent/EP2195612A2/de
Priority to JP2010527448A priority patent/JP2011503525A/ja
Publication of WO2009047189A2 publication Critical patent/WO2009047189A2/de
Publication of WO2009047189A3 publication Critical patent/WO2009047189A3/de
Priority to US12/753,349 priority patent/US8039815B2/en

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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G01N2021/653—Coherent methods [CARS]
    • G01N2021/655—Stimulated Raman
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01S—DEVICES USING THE PROCESS OF LIGHT AMPLIFICATION BY STIMULATED EMISSION OF RADIATION [LASER] TO AMPLIFY OR GENERATE LIGHT; DEVICES USING STIMULATED EMISSION OF ELECTROMAGNETIC RADIATION IN WAVE RANGES OTHER THAN OPTICAL
    • H01S3/00—Lasers, i.e. devices using stimulated emission of electromagnetic radiation in the infrared, visible or ultraviolet wave range
    • H01S3/30—Lasers, i.e. devices using stimulated emission of electromagnetic radiation in the infrared, visible or ultraviolet wave range using scattering effects, e.g. stimulated Brillouin or Raman effects
    • H01S3/302—Lasers, i.e. devices using stimulated emission of electromagnetic radiation in the infrared, visible or ultraviolet wave range using scattering effects, e.g. stimulated Brillouin or Raman effects in an optical fibre

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

Zum fluoreszenzlichtmikroskopischen Messen einer Probe (2) wird ein Fluoreszenzfarbstoff in der Probe (2) mit Licht (8) einer bestimmter Wellenlänge von einem Zustand in einen anderen Zustand überführt, wobei Licht (10) einer anderen Wellenlänge mit einer solchen Intensität in eine derart ausgewählte Lichtleiterfaser (13) eingekoppelt wird, dass in der Lichtleiterfaser (13) Raman-Streuung in solchem Ausmaß stimuliert wird, dass ein aus der Lichtleiterfaser (13) austretendes Lichtspektrum neben der eingekoppelten Wellenlänge mindestens eine rotverschobene Stokes-Linien (19 bis 28) aufweist, deren Intensitätshalbwertsbreite kleiner als ihr halber Abstand zu der er ihr im Blauen benachbarten Linie (18 bis 28) des Lichtspektrums (17) ist, und wobei die eine Wellenlänge aus einer der rotverschobenen Stokes-Linien (19 bis 28) ausgewählt wird; und Fluoreszenzlicht (6) von der Probe (2) wird ortsauflösend gemessen.
PCT/EP2008/063171 2007-10-05 2008-10-01 Fluoreszenzlichtmikroskopisches messen einer probe mit rotverschobenen stokes-linien WO2009047189A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP08837241A EP2195612A2 (de) 2007-10-05 2008-10-01 Fluoreszenzlichtmikroskopisches messen einer probe mit rotverschobenen stokes-linien
JP2010527448A JP2011503525A (ja) 2007-10-05 2008-10-01 赤方偏移したストローク線を用いる、試料の蛍光顕微鏡測定法
US12/753,349 US8039815B2 (en) 2007-10-05 2010-04-02 Fluorescent light microscope for measuring a sample using red-shifted stokes lines

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102007048135.9 2007-10-05
DE102007048135A DE102007048135B4 (de) 2007-10-05 2007-10-05 Fluoreszenzlichtmikroskopisches Messen einer Probe mit rotverschobenen Stokes-Linien

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/753,349 Continuation US8039815B2 (en) 2007-10-05 2010-04-02 Fluorescent light microscope for measuring a sample using red-shifted stokes lines

Publications (2)

Publication Number Publication Date
WO2009047189A2 WO2009047189A2 (de) 2009-04-16
WO2009047189A3 true WO2009047189A3 (de) 2009-09-03

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2008/063171 WO2009047189A2 (de) 2007-10-05 2008-10-01 Fluoreszenzlichtmikroskopisches messen einer probe mit rotverschobenen stokes-linien

Country Status (5)

Country Link
US (1) US8039815B2 (de)
EP (1) EP2195612A2 (de)
JP (1) JP2011503525A (de)
DE (1) DE102007048135B4 (de)
WO (1) WO2009047189A2 (de)

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DE202009007250U1 (de) 2009-05-20 2009-11-26 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Feldveränderungsmittel zur Erzeugung komplementärer Lichtintensitätsmuster
DE102009056058B4 (de) * 2009-11-25 2021-08-12 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Vorrichtung zur Erzeugung von Anregungsstrahlung für selbstkalibrierende faseroptische Raman-Sensoren, Messeinrichtung zum Erfassen der Temperatur eines Messobjekts und Raman-Sensoranordnung
DE102010037190B4 (de) 2010-08-27 2015-11-26 Leica Microsystems Cms Gmbh Vorrichtung zum zeitlichen Verschieben von Weißlichtlaserpulsen
FR2966292B1 (fr) * 2010-10-18 2013-01-18 Centre Nat Rech Scient Methode et dispositif d'emission laser pour l'analyse spectroscopique d'un echantillon
JP2012237714A (ja) * 2011-05-13 2012-12-06 Sony Corp 非線形ラマン分光装置、顕微分光装置及び顕微分光イメージング装置
CN102564642B (zh) * 2012-02-21 2013-08-07 中国计量学院 融合拉曼放大效应的光纤拉曼频移器的全分布光纤传感器
WO2014085695A1 (en) * 2012-11-28 2014-06-05 Trustees Of Princeton University Detection systems and methods using coherent anti-stokes raman spectroscopy
DE202013006817U1 (de) * 2013-07-30 2014-10-31 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Gepulste Laserlichtquelle für die Fluoreszenzanregung
JP6103008B2 (ja) * 2015-09-09 2017-03-29 ソニー株式会社 非線形ラマン分光装置、顕微分光装置及び顕微分光イメージング装置
DE102021128556A1 (de) 2021-11-03 2023-05-04 Amphos GmbH STED-Mikroskop
DE102021005684A1 (de) 2021-11-16 2023-05-17 Jörn Volkher Wochnowski STED-Verfahren mit Hohllichtwellenleitern

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Also Published As

Publication number Publication date
DE102007048135B4 (de) 2012-02-16
DE102007048135A1 (de) 2009-04-16
JP2011503525A (ja) 2011-01-27
EP2195612A2 (de) 2010-06-16
WO2009047189A2 (de) 2009-04-16
US8039815B2 (en) 2011-10-18
US20100187438A1 (en) 2010-07-29

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