WO2009041776A3 - Dna aptamer binding to oxytetracycline with specificity and production method thereof - Google Patents

Dna aptamer binding to oxytetracycline with specificity and production method thereof Download PDF

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Publication number
WO2009041776A3
WO2009041776A3 PCT/KR2008/005696 KR2008005696W WO2009041776A3 WO 2009041776 A3 WO2009041776 A3 WO 2009041776A3 KR 2008005696 W KR2008005696 W KR 2008005696W WO 2009041776 A3 WO2009041776 A3 WO 2009041776A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
otc
oxytetracycline
antibiotics
dna aptamer
Prior art date
Application number
PCT/KR2008/005696
Other languages
French (fr)
Other versions
WO2009041776A2 (en
Inventor
Man Bock Gu
M Javed Hossain Niazi K
Yeon Seok Kim
Yoonjin Kim
Su Jin Lee
Original Assignee
Univ Ind & Acad Collaboration
Man Bock Gu
M Javed Hossain Niazi K
Yeon Seok Kim
Yoonjin Kim
Su Jin Lee
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Ind & Acad Collaboration, Man Bock Gu, M Javed Hossain Niazi K, Yeon Seok Kim, Yoonjin Kim, Su Jin Lee filed Critical Univ Ind & Acad Collaboration
Publication of WO2009041776A2 publication Critical patent/WO2009041776A2/en
Publication of WO2009041776A3 publication Critical patent/WO2009041776A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)

Abstract

Provided is a DNA aptamer which binds specifically to Oxytetracycline (OTC). More particularly, there are provided a DNA aptamer which can bind specifically to OTC selected from a random DNA library, a method for producing the same, and a method for detecting or removing OTC using the same. DNA aptamers showing specifically high affinity for the antibiotics OTC were selected from a random DNA pool using the FluMag-SELEX process, a modification of the SELEX process. The selected DNA aptamers were confirmed to have strong binding affinity for OTC. Accordingly, the DNA aptamers provided herein may be used to effectively detect antibiotics remaining in drinking water sources, sewage, foods, human bodies, etc. Further, the DNA aptamers may be used to selectively remove a target substance from a sample containing a trace amount of antibiotics.
PCT/KR2008/005696 2007-09-27 2008-09-25 Dna aptamer binding to oxytetracycline with specificity and production method thereof WO2009041776A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20070097352A KR100930973B1 (en) 2007-09-27 2007-09-27 DNA aptamers that specifically bind to oxytetracycline and methods for preparing the same
KR10-2007-0097352 2007-09-27

Publications (2)

Publication Number Publication Date
WO2009041776A2 WO2009041776A2 (en) 2009-04-02
WO2009041776A3 true WO2009041776A3 (en) 2009-06-25

Family

ID=40512021

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2008/005696 WO2009041776A2 (en) 2007-09-27 2008-09-25 Dna aptamer binding to oxytetracycline with specificity and production method thereof

Country Status (2)

Country Link
KR (1) KR100930973B1 (en)
WO (1) WO2009041776A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101263449B1 (en) * 2010-12-30 2013-06-14 주식회사 아이센스 DNA aptamer binding to sulfadimethoxine with specificity
KR101342710B1 (en) 2012-02-14 2013-12-16 고려대학교 산학협력단 Nucleic Acid Aptamer Capable of Specifically Binding to Tetracyclines Compound and Use thereof
KR101528482B1 (en) * 2013-05-10 2015-06-15 호서대학교 산학협력단 Nucleic acid aptamer specifically binding to oxytetracycline
KR101460450B1 (en) * 2013-09-05 2014-11-12 충북대학교 산학협력단 DNA aptamer specifically binding to cadmium and uses thereof
KR101617205B1 (en) * 2013-10-16 2016-05-02 고려대학교 산학협력단 Nucleic Acid Aptamer Capable of Specifically Binding to Tebuconazole, Mefenacet and Inabenfide and Uses Thereof
KR101342080B1 (en) 2013-10-25 2013-12-18 고려대학교 산학협력단 Nucleic Acid Aptamer Capable of Specifically Binding to Tetracyclines Compound and Use thereof
KR101717767B1 (en) * 2014-03-12 2017-03-17 한국과학기술연구원 Universal nucleic acid aptamers for common binding to various microorganisms and production method thereof
CN106526168B (en) * 2016-11-10 2018-08-24 重庆师范大学 A kind of nucleotide aptamers probe reagent box of quick detection doxycycline remnant and its application
CN106823467A (en) * 2017-01-23 2017-06-13 北京美正生物科技有限公司 A kind of zearalenone aptamers affinity column and its production and use

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BERENS C ET AL.: "A tetracycline-binding RNA aptamer", BIOORGANIC & MEDICINAL CHEMISTRY, vol. 9, no. 10, October 2001 (2001-10-01), pages 2549 - 2556 *
NITSCHE A ET AL.: "One-step selection of vaccinia virus-binding DNA aptamers by MonoLEX", BMC BIOTECHNOLOGY, vol. 7, no. 48, August 2007 (2007-08-01), pages 1 - 12 *
STOLTENBURG R ET AL.: "FluMag-SELEX as an advantageous method for DNA aptamer selection", ANALYTICAL AND BIOANALYTICAL CHEMISTRY, vol. 383, no. 1, September 2005 (2005-09-01), pages 83 - 91 *

Also Published As

Publication number Publication date
KR20090032276A (en) 2009-04-01
KR100930973B1 (en) 2009-12-10
WO2009041776A2 (en) 2009-04-02

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