WO2009041032A1 - 蛍光検出方法 - Google Patents

蛍光検出方法 Download PDF

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Publication number
WO2009041032A1
WO2009041032A1 PCT/JP2008/002642 JP2008002642W WO2009041032A1 WO 2009041032 A1 WO2009041032 A1 WO 2009041032A1 JP 2008002642 W JP2008002642 W JP 2008002642W WO 2009041032 A1 WO2009041032 A1 WO 2009041032A1
Authority
WO
WIPO (PCT)
Prior art keywords
fluorescence intensity
microchip
living specimen
supplied
fluorescence
Prior art date
Application number
PCT/JP2008/002642
Other languages
English (en)
French (fr)
Inventor
Muneyasu Kimura
Original Assignee
Fujifilm Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujifilm Corporation filed Critical Fujifilm Corporation
Priority to US12/680,106 priority Critical patent/US8283640B2/en
Priority to EP08833299.4A priority patent/EP2194372B1/en
Publication of WO2009041032A1 publication Critical patent/WO2009041032A1/ja

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0346—Capillary cells; Microcells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Electrochemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optics & Photonics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

【課題】マイクロチップ自体の蛍光強度がバックグラウンドとして作用しても、高精度かつ短時間に蛍光標識された生体試料の蛍光強度を検出する蛍光検出方法を図る。 【解決手段】蛍光標識された生体試料（Ｒ）が供給されたマイクロチップ（１０）と同等のマイクロチップ（１１）自体から発生する蛍光（Ｌｋ０）の蛍光強度（Ｕｒ０）を励起光（Ｌｅ）の照射開始から蛍光強度（Ｕｒ０）が十分に減衰するまで検出し、蛍光強度（Ｕｒ０）の経時変化を記憶する。マイクロチップ（１０）に励起光（Ｌｅ）を照射すると共に、マイクロチップ（１０）自体からの蛍光強度（Ｕｒ０）が十分に減衰する前に、生体試料（Ｒ）を供給し、発生する蛍光強度（Ｕｒ）を励起光（Ｌｅ）の照射開始から生体試料（Ｒ）が供給された後まで検出する。生体試料（Ｒ）が供給されたマイクロチップ（１０）から検出された蛍光強度（Ｕｒ）から、記憶された蛍光強度（Ｕｒ０）の経時変化の値を減算して、生体試料（Ｒ）から発生した蛍光（Ｌｋ）の蛍光強度（Ｕｃ）を検出する。
PCT/JP2008/002642 2007-09-28 2008-09-24 蛍光検出方法 WO2009041032A1 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US12/680,106 US8283640B2 (en) 2007-09-28 2008-09-24 Fluorescence detection method
EP08833299.4A EP2194372B1 (en) 2007-09-28 2008-09-24 Fluorescence detection method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-255403 2007-09-28
JP2007255403A JP4993494B2 (ja) 2007-09-28 2007-09-28 蛍光検出方法

Publications (1)

Publication Number Publication Date
WO2009041032A1 true WO2009041032A1 (ja) 2009-04-02

Family

ID=40510937

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/002642 WO2009041032A1 (ja) 2007-09-28 2008-09-24 蛍光検出方法

Country Status (4)

Country Link
US (1) US8283640B2 (ja)
EP (1) EP2194372B1 (ja)
JP (1) JP4993494B2 (ja)
WO (1) WO2009041032A1 (ja)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103018218A (zh) * 2011-11-10 2013-04-03 浙江理工大学 一种用于洗涤剂洗涤效果检测和评价的荧光分析方法
CA3018582A1 (en) * 2016-03-25 2017-09-28 Bioceryx Inc. Apparatuses and methods for assessing target sequence numbers
CN107748148A (zh) * 2017-09-04 2018-03-02 江苏大学 基于石墨烯氧化物双氨基固定多t序列检测汞离子的方法
JP7282307B2 (ja) * 2019-05-20 2023-05-29 株式会社島津製作所 応力発光データ処理装置、応力発光データ処理方法、応力発光測定装置および応力発光試験システム

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002181708A (ja) 2000-12-14 2002-06-26 Asahi Techno Glass Corp マイクロアレイチップの読取方法及びその装置
JP2003084002A (ja) 2001-06-27 2003-03-19 Fuji Photo Film Co Ltd 蛍光検出におけるバックグラウンドの低減方法
JP2006226803A (ja) * 2005-02-17 2006-08-31 Matsushita Electric Ind Co Ltd 蛍光測定装置
JP2007278984A (ja) * 2006-04-11 2007-10-25 Hamamatsu Photonics Kk 光測定装置、光測定方法、及び光測定プログラム
JP2008122297A (ja) * 2006-11-14 2008-05-29 Fujitsu Ltd 蛍光検出装置および蛍光検出方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6485625B1 (en) * 1995-05-09 2002-11-26 Curagen Corporation Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments
US6333501B1 (en) * 2000-01-27 2001-12-25 Perkin-Elmer Corporation Methods, apparatus, and articles of manufacture for performing spectral calibration

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002181708A (ja) 2000-12-14 2002-06-26 Asahi Techno Glass Corp マイクロアレイチップの読取方法及びその装置
JP2003084002A (ja) 2001-06-27 2003-03-19 Fuji Photo Film Co Ltd 蛍光検出におけるバックグラウンドの低減方法
JP2006226803A (ja) * 2005-02-17 2006-08-31 Matsushita Electric Ind Co Ltd 蛍光測定装置
JP2007278984A (ja) * 2006-04-11 2007-10-25 Hamamatsu Photonics Kk 光測定装置、光測定方法、及び光測定プログラム
JP2008122297A (ja) * 2006-11-14 2008-05-29 Fujitsu Ltd 蛍光検出装置および蛍光検出方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2194372A4 *

Also Published As

Publication number Publication date
EP2194372A4 (en) 2013-01-09
JP4993494B2 (ja) 2012-08-08
EP2194372A1 (en) 2010-06-09
JP2009085756A (ja) 2009-04-23
EP2194372B1 (en) 2013-11-06
US8283640B2 (en) 2012-10-09
US20100258740A1 (en) 2010-10-14

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