WO2009032779A3 - Procédés et compositions utilisés dans la séparation, en fonction de sa taille, de l'acide nucléique d'un échantillon - Google Patents

Procédés et compositions utilisés dans la séparation, en fonction de sa taille, de l'acide nucléique d'un échantillon Download PDF

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Publication number
WO2009032779A3
WO2009032779A3 PCT/US2008/074689 US2008074689W WO2009032779A3 WO 2009032779 A3 WO2009032779 A3 WO 2009032779A3 US 2008074689 W US2008074689 W US 2008074689W WO 2009032779 A3 WO2009032779 A3 WO 2009032779A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
methods
seperation
compositions
sample
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Application number
PCT/US2008/074689
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English (en)
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WO2009032779A2 (fr
Inventor
Min Seob Lee
Original Assignee
Sequenom Inc
Min Seob Lee
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Publication date
Application filed by Sequenom Inc, Min Seob Lee filed Critical Sequenom Inc
Publication of WO2009032779A2 publication Critical patent/WO2009032779A2/fr
Publication of WO2009032779A3 publication Critical patent/WO2009032779A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Abstract

L'invention concerne des procédés et des trousses utilisés dans la séparation, l'extraction et l'enrichissement de l'acide nucléique à courte chaîne en présence d'un fond important d'un matériau génomique à longue chaîne (par exemple, des acides nucléiques hôtes ou maternels). Ces procédés reposent sur des amorces qui se lient sélectivement à l'acide nucléique à longue chaîne, ces amorces pouvant être utilisées pour séparer par la suite l'acide nucléique à longue chaîne et à courte chaîne.
PCT/US2008/074689 2007-08-29 2008-08-28 Procédés et compositions utilisés dans la séparation, en fonction de sa taille, de l'acide nucléique d'un échantillon WO2009032779A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US96887607P 2007-08-29 2007-08-29
US60/968,876 2007-08-29

Publications (2)

Publication Number Publication Date
WO2009032779A2 WO2009032779A2 (fr) 2009-03-12
WO2009032779A3 true WO2009032779A3 (fr) 2009-05-07

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Application Number Title Priority Date Filing Date
PCT/US2008/074689 WO2009032779A2 (fr) 2007-08-29 2008-08-28 Procédés et compositions utilisés dans la séparation, en fonction de sa taille, de l'acide nucléique d'un échantillon

Country Status (1)

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WO (1) WO2009032779A2 (fr)

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US9404150B2 (en) 2007-08-29 2016-08-02 Sequenom, Inc. Methods and compositions for universal size-specific PCR
US9430611B2 (en) 2005-11-26 2016-08-30 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US9580741B2 (en) 2009-04-03 2017-02-28 Sequenom, Inc. Nucleic acid preparation compositions and methods

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US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US10081839B2 (en) 2005-07-29 2018-09-25 Natera, Inc System and method for cleaning noisy genetic data and determining chromosome copy number
US10083273B2 (en) 2005-07-29 2018-09-25 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
EP3260556B1 (fr) 2006-05-31 2019-07-31 Sequenom, Inc. Procédés pour l'extraction d'acide nucléique à partir d'un échantillon
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
WO2008118988A1 (fr) 2007-03-26 2008-10-02 Sequenom, Inc. Détection de séquence polymorphe amplifiée par endonucléase de restriction
US7888127B2 (en) 2008-01-15 2011-02-15 Sequenom, Inc. Methods for reducing adduct formation for mass spectrometry analysis
CA2718137A1 (fr) 2008-03-26 2009-10-01 Sequenom, Inc. Detection de sequence polymorphique amplifiee par endonuclease de restriction
ES2620431T3 (es) 2008-08-04 2017-06-28 Natera, Inc. Métodos para la determinación de alelos y de ploidía
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
US8825412B2 (en) 2010-05-18 2014-09-02 Natera, Inc. Methods for non-invasive prenatal ploidy calling
EP2473638B1 (fr) 2009-09-30 2017-08-09 Natera, Inc. Méthode non invasive de détermination d'une ploïdie prénatale
DK2516680T3 (en) 2009-12-22 2016-05-02 Sequenom Inc Method and kits to identify aneuploidy
EP2569453B1 (fr) * 2010-05-14 2015-12-16 Fluidigm Corporation Méthodes d'isolement de l'acide nucléique
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
EP2656263B1 (fr) 2010-12-22 2019-11-06 Natera, Inc. Procédés de recherche de paternité prénatale, non invasive
JP6153874B2 (ja) 2011-02-09 2017-06-28 ナテラ， インコーポレイテッド 非侵襲的出生前倍数性呼び出しのための方法
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WO2013052907A2 (fr) 2011-10-06 2013-04-11 Sequenom, Inc. Méthodes et procédés pour évaluation non invasive de variations génétiques
US10424394B2 (en) 2011-10-06 2019-09-24 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9984198B2 (en) 2011-10-06 2018-05-29 Sequenom, Inc. Reducing sequence read count error in assessment of complex genetic variations
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ES2624686T3 (es) 2011-10-11 2017-07-17 Sequenom, Inc. Métodos y procesos para la evaluación no invasiva de variaciones genéticas
EP4148739A1 (fr) 2012-01-20 2023-03-15 Sequenom, Inc. Procédés de diagnostic qui factorent des conditions expérimentale
US9605313B2 (en) 2012-03-02 2017-03-28 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
DK2852680T3 (da) 2012-05-21 2020-03-16 Sequenom Inc Fremgangsmåder og processer til ikke-invasiv evaluering af genetiske variationer
US10497461B2 (en) 2012-06-22 2019-12-03 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
WO2014011928A1 (fr) 2012-07-13 2014-01-16 Sequenom, Inc. Procédés et compositions pour l'enrichissement basé sur la méthylation d'un échantillon maternel en acide nucléique fœtal, utiles pour les diagnostics prénatals non invasifs
KR101890466B1 (ko) * 2012-07-24 2018-08-21 내테라, 인코포레이티드 고도의 다중 ｐｃｒ 방법 및 조성물
EP4009329A1 (fr) 2012-10-04 2022-06-08 Sequenom, Inc. Méthodes et procédés d'évaluation non invasive de variations génétiques
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EP3597774A1 (fr) 2013-03-13 2020-01-22 Sequenom, Inc. Amorces pour analyse de la méthylation de l'adn
US9305756B2 (en) 2013-03-13 2016-04-05 Agena Bioscience, Inc. Preparation enhancements and methods of use for MALDI mass spectrometry
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CN112575075A (zh) 2013-05-24 2021-03-30 塞昆纳姆股份有限公司 遗传变异的非侵入性评估方法和过程
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CN106232812A (zh) * 2014-04-18 2016-12-14 凸版印刷株式会社 短链核酸的回收方法
RU2717641C2 (ru) 2014-04-21 2020-03-24 Натера, Инк. Обнаружение мутаций и плоидности в хромосомных сегментах
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EP3760739A1 (fr) 2014-07-30 2021-01-06 Sequenom, Inc. Méthodes et procédés d'évaluation non invasive de variations génétiques
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CA3030430A1 (fr) 2016-07-15 2018-01-18 The Regents Of The University Of California Procedes de production de bibliotheques d'acides nucleiques
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9430611B2 (en) 2005-11-26 2016-08-30 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US9404150B2 (en) 2007-08-29 2016-08-02 Sequenom, Inc. Methods and compositions for universal size-specific PCR
US9580741B2 (en) 2009-04-03 2017-02-28 Sequenom, Inc. Nucleic acid preparation compositions and methods
US9367663B2 (en) 2011-10-06 2016-06-14 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations

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