WO2009028692A1 - チアジアゾールを用いる生体分子の分離分析方法 - Google Patents

チアジアゾールを用いる生体分子の分離分析方法 Download PDF

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Publication number
WO2009028692A1
WO2009028692A1 PCT/JP2008/065602 JP2008065602W WO2009028692A1 WO 2009028692 A1 WO2009028692 A1 WO 2009028692A1 JP 2008065602 W JP2008065602 W JP 2008065602W WO 2009028692 A1 WO2009028692 A1 WO 2009028692A1
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WO
WIPO (PCT)
Prior art keywords
separation
biomolecular
analysis
biomolecule
analysis method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/065602
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English (en)
French (fr)
Inventor
Kuniaki Tatsuta
Yoshinori Yamaguchi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Waseda University
Original Assignee
Waseda University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Waseda University filed Critical Waseda University
Priority to JP2009530223A priority Critical patent/JPWO2009028692A1/ja
Publication of WO2009028692A1 publication Critical patent/WO2009028692A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/027—Liquid chromatography

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

生体分子を信頼性を確保したまま網羅的に分離分析するために、生化学サンプルの前処理を省略することができ、且つ、分離能をできるだけ高くすると同時に検出感度を高くできる分離溶媒及び分離方法を開発すること。 本発明は、チアジアゾールを含む溶媒に生体分子を可溶化するステップを含むことを特徴とする生体分子の分離分析方法と、チアジアゾールを含むことを特徴とする生体分子の分離分析用液体組成物と、該分離分析用液体組成物を含む生体分子分離分析用キットとを提供する。本発明の生体分子の分離分析方法は、前記溶媒に可溶化された生体分子を電気泳動法で分離するステップを含む場合がある。本発明の生体分子の分離分析方法は、前記溶媒に可溶化された生体分子をクロマトグラフィー法で分離するステップを含む場合がある。
PCT/JP2008/065602 2007-08-31 2008-08-29 チアジアゾールを用いる生体分子の分離分析方法 Ceased WO2009028692A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2009530223A JPWO2009028692A1 (ja) 2007-08-31 2008-08-29 チアジアゾールを用いる生体分子の分離分析方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007226301 2007-08-31
JP2007-226301 2007-08-31

Publications (1)

Publication Number Publication Date
WO2009028692A1 true WO2009028692A1 (ja) 2009-03-05

Family

ID=40387402

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/065602 Ceased WO2009028692A1 (ja) 2007-08-31 2008-08-29 チアジアゾールを用いる生体分子の分離分析方法

Country Status (2)

Country Link
JP (1) JPWO2009028692A1 (ja)
WO (1) WO2009028692A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015114150A (ja) * 2013-12-10 2015-06-22 株式会社島津製作所 電気泳動分離方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005003562A (ja) * 2003-06-13 2005-01-06 Hitachi High-Technologies Corp 電気泳動装置，キャピラリアレイ、及び電気泳動方法
JP2005211889A (ja) * 2004-01-28 2005-08-11 Univ Waseda １，２，５−チアジアゾール化合物を含有してなる溶剤およびこの溶媒を用いた有機化合物の抽出方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005003562A (ja) * 2003-06-13 2005-01-06 Hitachi High-Technologies Corp 電気泳動装置，キャピラリアレイ、及び電気泳動方法
JP2005211889A (ja) * 2004-01-28 2005-08-11 Univ Waseda １，２，５−チアジアゾール化合物を含有してなる溶剤およびこの溶媒を用いた有機化合物の抽出方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015114150A (ja) * 2013-12-10 2015-06-22 株式会社島津製作所 電気泳動分離方法

Also Published As

Publication number Publication date
JPWO2009028692A1 (ja) 2010-12-09

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