WO2009018695A1 - An enriching and screening bauxite desulfurizing bacteria method by using acidic water from the mining pit in high sulfur ore regions - Google Patents
An enriching and screening bauxite desulfurizing bacteria method by using acidic water from the mining pit in high sulfur ore regions Download PDFInfo
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- the invention relates to a method for enriching and sorting bauxite desulfurization bacteria, and more particularly to a method for enriching and screening bauxite desulfurization bacteria by utilizing acid mine water in a high sulfur mine area. Background technique
- the main sulfide minerals contained in the high-sulfur bauxite are pyrite (FeS 2 ), its isomerite pyrite and gum pyrite, and gypsum CaS0 4 -sulfate.
- FeS 2 pyrite
- the stone-filled minerals react with the alkali.
- the reaction mechanism is as follows: The iron stone U is first converted to sodium disulfide, and the sodium disulfide decomposes to form sodium sulfide at high temperature. Sodium thiosulfate is finally oxidized to sodium sulfate into the solution.
- Microorganisms can act on minerals in a variety of ways, converting elements in minerals into ions in solution.
- the research object of biometallurgy mainly uses iron and sulfur oxidizing bacteria to leaching almost all sulfide minerals such as copper, uranium, gold and nickel.
- the research on desulfurization of bauxite has not been reported in the literature. It has been reported that there are more than 20 kinds of microorganisms that can be used for leaching, mainly including ⁇ - ik ⁇ f 3 ⁇ 4 ⁇ Acidithiobacillus ferrooxidans, A. f), oxidized stone ⁇ A cidithiobacillus thiooxidans, A.
- biometallurgical-related bacteria are enriched and screened by the following methods: collecting water samples containing bacteria, inoculating a small amount of water in an iron-containing 9K medium for enrichment and screening, and then enriching the bacteria in the enriched solution multiple times. filter.
- the above method is a random screening, and the selected bacteria are not necessarily the required bacteria, so multiple enrichment screening is required. Summary of the invention
- the object of the present invention is to exist in the above biometallurgical related bacteria enrichment screening technology
- the shortcomings of the purpose are not strong, and the bacteria enrichment screening has the disadvantage of randomness.
- a method for the simple and rapid utilization of the acid mine pit water in the Gaoshike mining area for the enrichment and screening of bauxite deodorization bacteria is proposed.
- the method of the invention can be used for bauxite mining, especially high-sulfur bauxite desulfurization, after multiple enrichment screening and no need for enrichment screening for multiple domestication of bacteria.
- the method for enriching and screening the bauxite decalcification bacteria by using the acidic pit water in the Gaoshike mining area comprises the following steps: adding iron-free to the acidic pit water in the Gaoshike mining area of the Jiji
- the iron-free 9K inorganic salt component is: (NH 4 ) 2 S0 4 , K 2 HP0 4 , Ca(N0 3 ) 2 , MgS0 4 7H 2 0, and KC1.
- said 80 4 is 1:1 (by volume ratio of water) sulfuric acid.
- the high sulfur bauxite ore fines have a particle size of from -200 to 40 mesh.
- the high sulfur bauxite ore powder is added in an amount such that the concentration of the resulting slurry is 5 to 25% by weight.
- the sulfur content of the high-sulfur ore acid pit water is greater than 0.7% by weight; and the high sulfur bauxite ore powder has a sulfur content of more than 0.7% by weight.
- the slurry is placed in a gas bath thermostat for incubation.
- the gas bath constant temperature oscillator has a rotation speed of 100 to 200 rpm, a culture temperature of 25 to 50 ° C, and a culture time of 5 to 40 days.
- Bacteria are enriched and screened in a medium containing high-sulfur bauxite.
- the bacteria use sulfur or iron-bearing minerals in high-sulfur bauxite as energy to obtain high-sulfur bauxite.
- the bacteria are enriched and screened in a medium containing high-sulfur bauxite, the selected bacteria have a high ability to withstand pulp shearing, and can be used for high stone without multiple domestication. Desulphurization of bauxite.
- the bacteria in the acid mine pit water are enriched and screened by the high-sulfur bauxite mine in the mining area where the acid mine water is located.
- the obtained bacteria have better desulfurization effect on the high-sulfur bauxite ore than the high-sulfur bauxite mine in other mining areas.
- the acid mine pit water is enriched and screened for the obtained bacteria.
- the invention utilizes the acid mine pit water in the Gaoshike mining area to enrich and screen the bauxite desulfurization bacteria, and comprises the following steps: directly adding the following iron-free 9K inorganic salt component to the acidic mine pit water collected in the high sulfur mine area: (NH 4 ) 2 S0 4 l ⁇ 5g, K 2 HPO 4 0.2 ⁇ 0.7g, Ca(N0 3 ) 2 0.005 ⁇ 0.02g, MgS0 4 7H 2 0 0.2 ⁇ 0.7g, KC1 0.05 ⁇ 0.3g, with 1: 1H 2 S0 4 adjusts the pH to 1.2 ⁇ 3.0, and then adds high-sulfur bauxite ore powder with a particle size of -200-40.
- the concentration of the slurry formed after adding the ore powder is 5 ⁇ 25% by weight.
- the obtained slurry is cultured in a gas bath constant temperature oscillator at a temperature of 25 to 5 (TC, rotation speed of 100 to 200 rpm, and the culture time is 5 to 40 days, and the first enrichment screening mixed bacteria can be obtained.
- the first enrichment screening mixture is obtained.
- the Chongqing Gaoshike bauxite mine is used to carry out bacterial enrichment screening of the acidic pit water in the high-sulfur bauxite mining area of Chongqing, and the Chongqing high-sulfur bauxite mining area obtained by charging in a 250ml conical flask is used.
- Bacterial desulfurization of Chongqing high-sulfur bauxite was carried out by using mixed bacteria obtained by enrichment screening.
- 90 ml iron-free 9K medium ((NH 4 ) 2 S0 4 3g/L, K 2 HP0 4 0.5 was placed in a 250 ml Erlenmeyer flask.
- a 250 ml Erlenmeyer flask was filled with 90 ml of iron-free 9K medium ((NH 4 ) 2 S0 4 3g/L, K 2 HP0 4 0.5g/L, Ca(N0 3 ) 2 O.Olg/L, MgS0 4 7H 2 0 0.5g / L, KC1 O.lg / L, adjust the pH to 1.5 with 1 : 1 H 2 S0 4 , add lOg Nanchuan 1300 high-sulfur bauxite ore, adjust the pH of the leaching system with 1: 1 H 2 S0 4 , Shaker speed 180r / min, immersion temperature 30 ° C, inoculated 10ml enriched and screened bacteria (bacterial concentration in the inoculum was l.OxlO 8 / cm 3 ), immersed in a gas bath thermostat for 40 days
- the mineral has a sulfur content of 0.61%, a desulfurization rate of 87.2%, and an alumina recovery of
- the enriched liquid after enrichment and screening was filtered through a filter paper, filtered through a microporous membrane with a pore size of 0.22 ⁇ m, and collected in an iron-free 9K medium.
- a bacterial bacterial liquid having a bacterial concentration of l.OxlO 8 /cm 3 was obtained.
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Abstract
An enriching and screening bauxite desulfurizing bacteria method, using acidic water from the mining pit in high sulfur ore regions, which comprises the following steps: adding 9 k non-iron inorganic salts to the collected acidic water from the mining pit in high sulfur ore regions; then adjusting pH to 1.2-3.0 with H2SO4; adding the powdered ore of high sulfur bauxite to form mining slurry; culturing the mining slurry to enrich and screen bacteria from acidic water of the mining pit in high sulfur ore regions.
Description
利用高石克矿区酸性矿坑水对铝土矿脱石 Ji细菌进行富集筛选的方法 技术领域 Method for enriching and screening of bauxite decalcification Ji bacteria by using acid pit water in Gaoshike mining area
本发明涉及一种对铝土矿脱硫细菌进行富集薛选的方法， 更具体 而言 , 涉及一种利用高硫矿区酸性矿坑水对铝土矿脱硫细菌进行富集 筛选的方法。 背景技术 The invention relates to a method for enriching and sorting bauxite desulfurization bacteria, and more particularly to a method for enriching and screening bauxite desulfurization bacteria by utilizing acid mine water in a high sulfur mine area. Background technique
高硫型铝土矿含有的主要硫化矿物有黄铁矿 （FeS2 ) 、 其异构体 白铁矿和胶黄铁矿以及石膏 CaS04—类硫酸盐。 拜耳法处理高硫型铝 土矿时， 其中的石充化矿物与碱发生反应， 其反应机理为： 铁的石 U匕物 首先转化为二硫化钠， 高温下二硫化钠分解生成硫化钠和硫代硫酸 钠， 最终氧化成硫酸钠进入溶液中。 由于硫酸钠在氧化铝生产中会逐 渐积累， 给氧化铝生产和操作带来不少困难和危害， 因此高硫铝土矿 需通过脱硫处理后再进行利用。 拜耳法生产要求矿石中的硫含量低于 0.7 % , 甚至越低越好。 有效排除高硫铝土矿中的硫杂质， 对开辟新的 氧化铝生产资源显得十分必要。 The main sulfide minerals contained in the high-sulfur bauxite are pyrite (FeS 2 ), its isomerite pyrite and gum pyrite, and gypsum CaS0 4 -sulfate. When the Bayer process is used to treat high-sulfur bauxite, the stone-filled minerals react with the alkali. The reaction mechanism is as follows: The iron stone U is first converted to sodium disulfide, and the sodium disulfide decomposes to form sodium sulfide at high temperature. Sodium thiosulfate is finally oxidized to sodium sulfate into the solution. Since sodium sulfate will gradually accumulate in the production of alumina, it brings many difficulties and harms to the production and operation of alumina. Therefore, the high-sulfur bauxite ore needs to be desulfurized before being used. Bayer production requires that the sulphur content of the ore be less than 0.7%, even as low as possible. Effective elimination of sulfur impurities in high-sulfur bauxite mines is necessary to open up new alumina production resources.
微生物可以通过多种途径对矿物作用， 将矿物中的元素转化为溶 液中的离子。 目前生物冶金的研究对象主要是利用铁、 硫氧化细菌进 行铜、 铀、 金、 镍等几乎所有硫化矿物的浸出， 但对铝土矿脱硫的研 究尚未见文献报道。 已报道可用于浸矿的微生物有 20 多种， 主要有 ^^ ^- ik^^f ¾ {Acidithiobacillus ferrooxidans, A. f)、 氧化石克疏軒菌 {A cidithiobacillus thiooxidans, A. i)、 氧化亚铁钩端虫累菌 ( ep s ^W〃ww ferrooxidans, L. f)。 研究细菌浸出脱除高硫铝土矿中的杂质硫， 对高硫 铝土矿的高效利用具有十分重大的意义。 Microorganisms can act on minerals in a variety of ways, converting elements in minerals into ions in solution. At present, the research object of biometallurgy mainly uses iron and sulfur oxidizing bacteria to leaching almost all sulfide minerals such as copper, uranium, gold and nickel. However, the research on desulfurization of bauxite has not been reported in the literature. It has been reported that there are more than 20 kinds of microorganisms that can be used for leaching, mainly including ^^^- ik^^f 3⁄4 {Acidithiobacillus ferrooxidans, A. f), oxidized stone {A cidithiobacillus thiooxidans, A. i), oxidation Helicobacter pylori (ep s ^W〃ww ferrooxidans, L. f). Studying bacterial leaching to remove impurity sulfur from high-sulfur bauxite is of great significance for the efficient use of high-sulfur bauxite.
目前生物冶金相关细菌均采用以下方法进行富集筛选： 采集含有 细菌的水样， 接种少量水样于含铁 9K 培养基中进行富集筛选， 再对 富集液中的细菌多次进行富集筛选。 上述方法为随机筛选， 筛选出的 细菌并不一定为所需要的细菌， 因此需要多次富集筛选。 发明内容 At present, biometallurgical-related bacteria are enriched and screened by the following methods: collecting water samples containing bacteria, inoculating a small amount of water in an iron-containing 9K medium for enrichment and screening, and then enriching the bacteria in the enriched solution multiple times. filter. The above method is a random screening, and the selected bacteria are not necessarily the required bacteria, so multiple enrichment screening is required. Summary of the invention
本发明的目的是针对上述生物冶金相关细菌富集筛选技术中存在
的目的性不强、 细菌富集筛选存在随机性的缺点， 提出一种能简单、 快速的利用高石克矿区酸性矿坑水进行铝土矿脱 υ细菌富集筛选的方 法。 The object of the present invention is to exist in the above biometallurgical related bacteria enrichment screening technology The shortcomings of the purpose are not strong, and the bacteria enrichment screening has the disadvantage of randomness. A method for the simple and rapid utilization of the acid mine pit water in the Gaoshike mining area for the enrichment and screening of bauxite deodorization bacteria is proposed.
本发明的方法， 无需多次富集筛选以及无需富集筛选后对细菌进 行多次驯化， 经过一次细菌富集筛选后即可用于铝土矿， 尤其是高硫 铝土矿的脱硫。 The method of the invention can be used for bauxite mining, especially high-sulfur bauxite desulfurization, after multiple enrichment screening and no need for enrichment screening for multiple domestication of bacteria.
本发明的目的是通过以下技术方案实现的。 The object of the present invention is achieved by the following technical solutions.
本发明的利用高石克矿区酸性矿坑水对铝土矿脱石克细菌进行富集筛 选的方法， 包括以下步骤： 向釆集的高石克矿区酸性矿坑水中加入无铁 The method for enriching and screening the bauxite decalcification bacteria by using the acidic pit water in the Gaoshike mining area comprises the following steps: adding iron-free to the acidic pit water in the Gaoshike mining area of the Jiji
9Κ无机盐， 然后用 H2S04调节 pH 为 1.2~3.0， 再加入高硫铝土矿矿 粉形成浆液， 将上述浆液进行培养以对高硫矿区酸性矿坑水中的细菌 进行富集筛选。 9Κ inorganic salt, then adjust the pH to 1.2~3.0 with H 2 S0 4 , then add high-sulfur bauxite ore powder to form a slurry, and the above slurry is cultured to enrich and screen the bacteria in the acidic pit water of the high-sulfur mine area.
根据本发明的一个实施方案， 其中所述无铁 9K 无机盐成分为： (NH4)2S04、 K2HP04、 Ca(N03)2、 MgS047H20和 KC1。 According to an embodiment of the present invention, the iron-free 9K inorganic salt component is: (NH 4 ) 2 S0 4 , K 2 HP0 4 , Ca(N0 3 ) 2 , MgS0 4 7H 2 0, and KC1.
根据本发明的另一实施方案， 其中所述 804为 1 :1 (与水的体 积比） 的硫酸。 According to another embodiment of the invention, wherein said 80 4 is 1:1 (by volume ratio of water) sulfuric acid.
根据本发明的又一实施方案， 其中所述高硫铝土矿矿粉的粒径为- 200— 40目。 According to still another embodiment of the present invention, the high sulfur bauxite ore fines have a particle size of from -200 to 40 mesh.
根据本发明的再一实施方案， 其中所述高硫铝土矿矿粉的加入量 使得所得浆液的浓度为 5~25重量％。 According to still another embodiment of the present invention, the high sulfur bauxite ore powder is added in an amount such that the concentration of the resulting slurry is 5 to 25% by weight.
根据本发明的又一实施方案， 其中所述高硫矿区酸性矿坑水的含 硫量大于 0.7重量％; 所述高硫铝土矿矿粉的含硫量大于 0.7重量％。 According to still another embodiment of the present invention, the sulfur content of the high-sulfur ore acid pit water is greater than 0.7% by weight; and the high sulfur bauxite ore powder has a sulfur content of more than 0.7% by weight.
根据本发明的另一实施方案， 其中将所述浆液置于气浴恒温振荡 器中进行培养。 According to another embodiment of the invention, the slurry is placed in a gas bath thermostat for incubation.
根据本发明的又一实施方案， 其中气浴恒温振荡器的转速为 100~200rpm, 培养温度为 25〜50 °C， 培养时间为 5〜40天。 According to still another embodiment of the present invention, the gas bath constant temperature oscillator has a rotation speed of 100 to 200 rpm, a culture temperature of 25 to 50 ° C, and a culture time of 5 to 40 days.
采用本发明选育的高硫铝土矿脱 细菌有以下特点： The high sulfur bauxite ore bacterium selected by the present invention has the following characteristics:
( 1 ) 富集筛选得到的为混合细菌， 在多种细菌组成的小生境中， 细菌间的相互作用使得细菌始终保持高活性， 从而使微生物脱硫的效 果明显提高； 另外混合细菌也更易于保藏； (1) Enrichment screening results in mixed bacteria. In a small habitat composed of various bacteria, the interaction between bacteria makes the bacteria always maintain high activity, so that the effect of microbial desulfurization is obviously improved; in addition, the mixed bacteria are also easier to preserve. ;
( 2 ) 细菌是在含有高硫铝土矿的培养基中富集筛选得到的， 细 菌以高硫铝土矿中的含硫或含铁矿物为能源， 从而得到对高硫铝土矿
中含硫杂质专性浸出的细菌； (2) Bacteria are enriched and screened in a medium containing high-sulfur bauxite. The bacteria use sulfur or iron-bearing minerals in high-sulfur bauxite as energy to obtain high-sulfur bauxite. An occluded bacteria containing sulfur impurities;
( 3 ) 由于细菌是在含有高硫铝土矿的培养基中富集筛选得到的， 因此筛选得到的细菌具有较高的耐受矿浆剪切的能力， 不需多次驯化 即可用于高石克铝土矿脱硫。 (3) Since the bacteria are enriched and screened in a medium containing high-sulfur bauxite, the selected bacteria have a high ability to withstand pulp shearing, and can be used for high stone without multiple domestication. Desulphurization of bauxite.
( 4 ) 利用酸性矿坑水所在矿区的高硫铝土矿对酸性矿坑水中的 细菌进行富集筛选， 所得细菌对高硫铝土矿的脱硫效果要优于利用其 它矿区的高硫铝土矿对酸性矿坑水富集筛选得到的细菌。 具体实施方式 (4) The bacteria in the acid mine pit water are enriched and screened by the high-sulfur bauxite mine in the mining area where the acid mine water is located. The obtained bacteria have better desulfurization effect on the high-sulfur bauxite ore than the high-sulfur bauxite mine in other mining areas. The acid mine pit water is enriched and screened for the obtained bacteria. detailed description
本发明利用高石克矿区酸性矿坑水对铝土矿脱石克细菌进行富集筛选 的方法， 包括以下步骤： 将采集于高硫矿区的酸性矿坑水中直接加入 以下无铁 9K无机盐成分： (NH4)2S04 l~5g， K2HPO4 0.2〜0.7g， Ca(N03)2 0.005~0.02g, MgS047H20 0.2〜0.7g, KC1 0.05〜0.3g, 用 1 : 1H2S04将 pH调为 1.2~3.0， 再加入粒径为 -200— 40 目的高硫铝土矿矿粉， 加入 矿粉后形成的浆液浓度为 5〜25重量％。 将所得浆液置于气浴恒温振荡 器中培养， 温度为 25~5(TC， 转速为 100~200rpm, 培养时间 5〜40天 后即可得到初次富集筛选混合细菌。 将初次富集筛选的混合细菌经过 滤纸过滤、 孔为 0.22 μ ιη 的微孔滤膜过滤后收集菌种， 然后用 ρΗ=1.0-2.5 的去离子水洗涤微孔滤膜， 最后用无铁 9Κ培养基将细菌 沖下， 即可得到用于高 υ铝土矿脱硫的细菌。 实施例 The invention utilizes the acid mine pit water in the Gaoshike mining area to enrich and screen the bauxite desulfurization bacteria, and comprises the following steps: directly adding the following iron-free 9K inorganic salt component to the acidic mine pit water collected in the high sulfur mine area: (NH 4 ) 2 S0 4 l~5g, K 2 HPO 4 0.2~0.7g, Ca(N0 3 ) 2 0.005~0.02g, MgS0 4 7H 2 0 0.2~0.7g, KC1 0.05~0.3g, with 1: 1H 2 S0 4 adjusts the pH to 1.2~3.0, and then adds high-sulfur bauxite ore powder with a particle size of -200-40. The concentration of the slurry formed after adding the ore powder is 5~25% by weight. The obtained slurry is cultured in a gas bath constant temperature oscillator at a temperature of 25 to 5 (TC, rotation speed of 100 to 200 rpm, and the culture time is 5 to 40 days, and the first enrichment screening mixed bacteria can be obtained. The first enrichment screening mixture is obtained. The bacteria were filtered through a filter paper, filtered through a microporous membrane with a pore size of 0.22 μm, and the strain was collected. Then, the microporous membrane was washed with deionized water of ρΗ=1.0-2.5, and finally the bacteria were washed down with an iron-free medium. Bacteria for desulfurization of sorghum bauxite can be obtained.
表 1 高硫铝土矿主要成分含量 （重量％) Table 1 Content of main components of high-sulfur bauxite (% by weight)
实施例 1 Example 1
用本发明所述方法， 利用重庆高石克铝土矿对重庆高硫铝土矿区酸 性矿坑水进行细菌富集筛选， 在 250ml锥形瓶中装入釆集得到的重庆 高硫铝土矿区酸性矿坑水 100ml , 按本发明所述方法加入无铁 9K 无 机盐成分： (NH4)2S04 3g, K2HP04 0.5g, Ca(N03)2 0.0 lg, MgS047H20 0.5g，KC1 0.1g,用 1 : 1 H2S04调节 pH为 1.5 ,再加入 lOg粒径为 -200~-40
目的重庆高硫铝土矿矿粉形成浆液， 摇床转速 180r/min、 温度 30°C下 进行细菌富集筛选， 培养 30 天后得到富集筛选后的混合细菌。 利用 富集筛选得到的混合细菌对重庆高硫铝土矿进行细菌脱硫， 在 250ml 锥形瓶中装入 90ml无铁 9K培养基 ( (NH4)2S04 3g/L, K2HP04 0.5g/L, Ca(N03)2 O.Olg/L, MgS047H20 0.5g/L， C1 O.lg/L, 用 H2S04调 节 pH为 1.5 ) ， 加入 lOg重庆高硫铝 矿， 用 1 :1 H2S04调节浸出体 系 pH 1.5， 摇床转速 180r/min， 浸矿温度 30°C， 接种 10ml富集筛选 后的细菌 （接种液中细菌浓度为 l .OxlO8个 /cm3 ) 。 于气浴恒温振荡器 中浸出 20天后矿物的含硫量为 1.88%， 脱硫率为 67.2%; 浸出 40天 后矿物的含硫量为 0.36%， 脱石克率为 93.7%， 氧化铝回收率为 96.1%。 Using the method of the invention, the Chongqing Gaoshike bauxite mine is used to carry out bacterial enrichment screening of the acidic pit water in the high-sulfur bauxite mining area of Chongqing, and the Chongqing high-sulfur bauxite mining area obtained by charging in a 250ml conical flask is used. 100 ml of acid pit water, adding iron-free 9K inorganic salt component according to the method of the invention: (NH 4 ) 2 S0 4 3g, K 2 HP0 4 0.5g, Ca(N0 3 ) 2 0.0 lg, MgS0 4 7H 2 0 0.5 g, KC1 0.1g, adjust the pH to 1.5 with 1: 1 H 2 S0 4 , and add lOg particle size of -200~-40 Objective To form a slurry of high-sulfur bauxite ore in Chongqing, and to carry out bacterial enrichment screening at a shaking speed of 180r/min and a temperature of 30°C. After 30 days of culture, the mixed bacteria after enrichment and screening were obtained. Bacterial desulfurization of Chongqing high-sulfur bauxite was carried out by using mixed bacteria obtained by enrichment screening. 90 ml iron-free 9K medium ((NH 4 ) 2 S0 4 3g/L, K 2 HP0 4 0.5 was placed in a 250 ml Erlenmeyer flask. g/L, Ca(N0 3 ) 2 O.Olg/L, MgS0 4 7H 2 0 0.5g/L, C1 O.lg/L, pH adjusted to 1.5 with H 2 S0 4 , add lOg Chongqing high sulfur aluminum Mine, adjust the leaching system pH 1.5 with 1:1 H 2 S0 4 , shaker speed 180r/min, immersion temperature 30 °C, inoculate 10ml enriched and screened bacteria (bacteria concentration in inoculum is l.OxlO 8 /cm 3 ). After 20 days of leaching in a gas bath thermostat, the sulphur content of the mineral was 1.88%, and the desulfurization rate was 67.2%. After 40 days of leaching, the sulphur content of the mineral was 0.36%, the desilvering rate was 93.7%, and the alumina recovery rate was 93.7%. 96.1%.
实施例 2 Example 2
利用重庆高硫铝土矿对重庆高石 铝土矿区酸性矿坑水进行细菌富 集筛选， 方法同实施例 1 , 利用富集筛选得到的混合细菌对南川 1300 高硫铝土矿进行细菌脱硫， 在 250ml锥形瓶中装入 90ml无铁 9K培养 基 （（NH4)2S04 3g/L， K2HP04 0.5g/L, Ca(N03)2 O.Olg/L, MgS047H20 0.5g/L, KC1 O.lg/L, 用 1 : 1 H2S04调节 pH为 1.5 )， 加入 lOg南川 1300 高硫铝土矿， 用 1 : 1 H2S04调节浸出体系 pH 1.5 , 摇床转速 180r/min， 浸矿温度 30°C， 接种 10ml 富集筛选后的细菌 （接种液中细菌浓度为 l .OxlO8个 /cm3 ) ， 于气浴恒温振荡器中浸出 40 天后矿物的含硫量为 0.61%, 脱硫率为 87.2%， 氧化铝回收率为 97.5%。 Using Chongqing high-sulfur bauxite ore to carry out bacterial enrichment screening of acid mine pit water in Chongqing Gaoshi Bauxite Mine. The method is the same as in Example 1. The bacteria from the Nanchuan 1300 high-sulfur bauxite mine is desulfurized by using the mixed bacteria obtained by enrichment and screening. A 250 ml Erlenmeyer flask was filled with 90 ml of iron-free 9K medium ((NH 4 ) 2 S0 4 3g/L, K 2 HP0 4 0.5g/L, Ca(N0 3 ) 2 O.Olg/L, MgS0 4 7H 2 0 0.5g / L, KC1 O.lg / L, adjust the pH to 1.5 with 1 : 1 H 2 S0 4 , add lOg Nanchuan 1300 high-sulfur bauxite ore, adjust the pH of the leaching system with 1: 1 H 2 S0 4 , Shaker speed 180r / min, immersion temperature 30 ° C, inoculated 10ml enriched and screened bacteria (bacterial concentration in the inoculum was l.OxlO 8 / cm 3 ), immersed in a gas bath thermostat for 40 days The mineral has a sulfur content of 0.61%, a desulfurization rate of 87.2%, and an alumina recovery of 97.5%.
对比例 3 Comparative example 3
利用传统方法进行细菌富集筛选， 首先在 90ml 9K 培养基 ( (NH4)2S04 3g/L , K2HP04 0.5g/L , Ca(N03)2 O.Olg/L , MgS047H20 0.5g/L, KC1 O.lg/L, 用 1 : 1 H2S04调节 pH为 1.5 ) 中加入 10ml重庆 高硫铝土矿区酸性矿坑水， 于摇床转速 100~200r/min、 、 温度 30°C下 进行， 富集筛选 10 天后得到富集筛选后的细菌富集液， 接种富集筛 选后的细菌富集液 10ml于 90ml 9K培养基 ( (NH4)2S04 3g/L, K2HP04 0.5g/L, Ca(N03)2 O.Olg/L, MgS047H20 0.5g/L, KC1 O.lg/L,用 1 : 1 H2S04 调节 pH为 1.5 ) 中再次进行富集筛选 5天， 如此重复二次， 富集筛选 后的富集液经过滤纸过滤、 孔为 0.22 μ ηι的微孔滤膜过滤后用无铁 9K 培养基收集菌种， 得到细菌浓度为 l .OxlO8个 /cm3的细菌菌液。 利用此 富集筛选细菌进行重庆高硫铝土矿细菌脱硫， 用 1 : 1 H2S04调节浸出
体系 pH至 1.5,摇床转速 100〜200r/min,浸矿温度 25~50°C,接种 10ml 富集筛选后的细菌 （接种液中细菌浓度为 l.OxlO8个 /cm3) ， 浸出 40 天后矿物的含硫量为 2.80%,脱硫率为 51.1%，氧化铝回收率为 97.2%。
Bacterial enrichment screening using traditional methods, first in 90ml 9K medium ((NH 4 ) 2 S0 4 3g / L , K 2 HP0 4 0.5g / L , Ca (N0 3 ) 2 O.Olg / L , MgS0 4 7H 2 0 0.5g/L, KC1 O.lg/L, adjust the pH to 1.5 with 1: 1 H 2 S0 4 ) Add 10ml of acidic pit water in Chongqing high-sulfur bauxite mining area, the shaking speed of the shaker is 100~200r/ After min, and at a temperature of 30 ° C, the enriched and screened bacterial enrichment solution was obtained after 10 days of enrichment screening, and 10 ml of the enriched and enriched bacterial enrichment solution was inoculated into 90 ml of 9K medium ((NH 4 ) 2 S0 4 3g/L, K 2 HP0 4 0.5g/L, Ca(N0 3 ) 2 O.Olg/L, MgS0 4 7H 2 0 0.5g/L, KC1 O.lg/L, with 1: 1 H 2 S0 4 The pH was adjusted to 1.5) and the enrichment screen was again performed for 5 days. This was repeated twice. The enriched liquid after enrichment and screening was filtered through a filter paper, filtered through a microporous membrane with a pore size of 0.22 μm, and collected in an iron-free 9K medium. For the bacterial species, a bacterial bacterial liquid having a bacterial concentration of l.OxlO 8 /cm 3 was obtained. Using this enrichment to screen bacteria for bacterial desulfurization of high-sulfur bauxite in Chongqing, and leaching with 1: 1 H 2 S0 4 The pH of the system is 1.5, the shaking speed is 100~200r/min, the immersion temperature is 25~50°C, and 10ml of bacteria are enriched and screened (the concentration of bacteria in the inoculum is l.OxlO 8 /cm 3 ), leaching 40 The sulphur content of the minerals was 2.80%, the desulfurization rate was 51.1%, and the alumina recovery was 97.2%.
Claims
1. 利用高硫矿区酸性矿坑水对铝土矿脱石克细菌进行富集筛选的 方法， 包括以下步骤： 向采集的高硫矿区酸性矿坑水中加入无铁 9K 无机盐， 然后用 H2S04调节 pH为 1.2~3.0， 再加入高硫铝土矿矿粉形 成浆液， 将上述浆液进行培养以对高硫矿区酸性矿坑水中的细菌进行 冨集筛选。 1. The method for enriching and screening bauxite from the bauxite in the high-sulphur mine acid pit water, comprising the following steps: adding iron-free 9K inorganic salt to the collected high-sulfur mine acid pit water, and then using H 2 S0 4 The pH is adjusted to 1.2~3.0, and then the high-sulfur bauxite ore powder is added to form a slurry, and the slurry is cultured to collect and screen the bacteria in the acidic pit water of the high-sulfur mine area.
2. 根据权利要求 1所述的方法， 其中所述无铁 9K无机盐成分为： (NH4)2S04卜 5g、 2HP04 0.2~0.7g、 Ca(N03)2 0.005〜0.02g、 MgS047H20 0.2〜0.7g、 KC1 0.05〜0.3g。 2. The method according to claim 1, wherein the iron-free 9K inorganic salt component is: (NH 4 ) 2 S0 4 b 5g, 2 HP0 4 0.2-0.7 g, Ca(N0 3 ) 2 0.005-0.02 g , MgS0 4 7H 2 0 0.2~0.7g, KC1 0.05~0.3g.
3. 根据权利要求 1 或 2的方法， 其中所述 H2S04为 1 :1 (与水的 体积比） 的 酸。 3. The method according to claim 1 or 2, wherein the H 2 S0 4 is an acid of 1:1 (by volume ratio with water).
4. 根据权利要求 1-3任一项所述的方法， 其中所述高硫铝土矿矿 粉的粒径为 -200〜- 40目。 The method according to any one of claims 1 to 3, wherein the high-sulfur bauxite ore has a particle diameter of -200 to 40 mesh.
5. 根据权利要求 1-4任一项所述的方法， 其中所述高硫铝土矿矿 粉的加入量使得所得浆液的浓度为 5〜25重量％。 The method according to any one of claims 1 to 4, wherein the high-sulfur bauxite ore is added in an amount such that the concentration of the resulting slurry is 5 to 25% by weight.
6. 根据权利要求 1 -5任一项所述的方法， 其中所述高硫矿区酸性 矿坑水的含硫量大于 0.7重量％；所述高硫铝土矿矿粉的含硫量大于 0.7 重量％。 The method according to any one of claims 1 to 5, wherein the sulfur content of the high-sulfur ore acid pit water is greater than 0.7% by weight; the sulfur content of the high-sulfur bauxite ore powder is greater than 0.7 weight %.
7. 根据权利要求 1-6任一项所述的方法， 其中将所述浆液置于气 浴恒温振荡器中进行培养。 The method according to any one of claims 1 to 6, wherein the slurry is placed in a gas bath thermostat for cultivation.
8. 根据权利要求 Ί 所述的方法， 其中气浴恒温振荡器的转速为 100〜200rpm， 培养温度为 25〜50 °C , 培养时间为 5〜40天。
8. The method according to claim ,, wherein the gas bath constant temperature oscillator has a rotation speed of 100 to 200 rpm, a culture temperature of 25 to 50 ° C, and a culture time of 5 to 40 days.
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| CN102897812A (en) * | 2012-10-19 | 2013-01-30 | 北京化工大学 | Method for activating high-sulfur bauxite by means of low-temperature roasting desulfurization method |
| WO2022012904A1 (en) | 2020-07-13 | 2022-01-20 | Unilever Ip Holdings B.V. | Antiperspirant stick compositions |
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| CN1475559A (en) * | 2002-08-15 | 2004-02-18 | 北京有色金属研究总院 | Breeding method of acid resistant mutagenic ore immersion bacterial culture |
| CN1924042A (en) * | 2006-10-13 | 2007-03-07 | 中国铝业股份有限公司 | Method of bacterial eliminating sulfur in high sulfur bauxite |
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| CN1475559A (en) * | 2002-08-15 | 2004-02-18 | 北京有色金属研究总院 | Breeding method of acid resistant mutagenic ore immersion bacterial culture |
| CN1924042A (en) * | 2006-10-13 | 2007-03-07 | 中国铝业股份有限公司 | Method of bacterial eliminating sulfur in high sulfur bauxite |
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| CN102897812A (en) * | 2012-10-19 | 2013-01-30 | 北京化工大学 | Method for activating high-sulfur bauxite by means of low-temperature roasting desulfurization method |
| WO2022012904A1 (en) | 2020-07-13 | 2022-01-20 | Unilever Ip Holdings B.V. | Antiperspirant stick compositions |
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