WO2009003341A1 - Engrais chimique composite comprenant des matériaux bioactifs - Google Patents

Engrais chimique composite comprenant des matériaux bioactifs Download PDF

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Publication number
WO2009003341A1
WO2009003341A1 PCT/CN2007/070228 CN2007070228W WO2009003341A1 WO 2009003341 A1 WO2009003341 A1 WO 2009003341A1 CN 2007070228 W CN2007070228 W CN 2007070228W WO 2009003341 A1 WO2009003341 A1 WO 2009003341A1
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WO
WIPO (PCT)
Prior art keywords
fermentation
microorganisms
temperature
fertilizer
chemical fertilizer
Prior art date
Application number
PCT/CN2007/070228
Other languages
English (en)
Chinese (zh)
Inventor
Qingli Yang
Original Assignee
Qingli Yang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qingli Yang filed Critical Qingli Yang
Priority to PCT/CN2007/070228 priority Critical patent/WO2009003341A1/fr
Publication of WO2009003341A1 publication Critical patent/WO2009003341A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners

Definitions

  • the present invention relates to a composite chemical fertilizer containing a biologically active substance.
  • An object of the present invention is to provide a fertilizer having both biological and chemical properties, and a composite chemical fertilizer containing a biologically active substance is prepared by adding an activator containing an active microorganism in a dormant state and an organic filler.
  • a compound chemical fertilizer containing a biologically active substance comprising: a biologically active activator, an organic filler and a fertilizer having a total active ingredient of not less than 25%,
  • an activator containing more than 1 billion Bacillus subtilis and 20-45 parts by weight of an organic filler added to parts by weight of 50-70 nitrogen, phosphorus, potassium chemical fertilizer or In the compound, it is uniformly mixed in a high-speed shearing machine, granulated by a granulator, and dried to obtain a granular fertilizer.
  • a compound chemical fertilizer containing a biologically active substance which is a microorganism capable of stably surviving in a high osmotic pressure environment formed by a chemical fertilizer, and particularly refers to a state-approved agricultural beneficial microorganism Bacillus subtilis in a dormant state, Bacillus Subtilis.
  • the composite chemical fertilizer containing a biologically active substance wherein the activator refers to a substance which is in a dormant state by an agriculturally beneficial microorganism Bacillus subtilis through an expansion culture and a sporulation process.
  • the activator refers to a substance which is in a dormant state by an agriculturally beneficial microorganism Bacillus subtilis through an expansion culture and a sporulation process.
  • This new fertilizer meets the following requirements: [8] Microorganisms used in this bioengineering method to modify common chemical fertilizers must be microorganisms that can survive in the high osmotic environment formed by chemical fertilizers, such as state-approved agricultural beneficial microorganisms in dormant state. Bacillus subtilis, Bacillus Subtilis
  • Cultivation by a specific method ensures that a certain amount of living microorganisms are retained in the final finished product.
  • the living microorganism can survive a sufficiently long mash in the newly formed microbial-fertilizer-organic filler ternary composite system to avoid the microbial activity of the newly produced finished product.
  • the transition between the daytime and the gradual death of the microorganisms and the disappearance of the activity, the active day referred to here is more than one year, that is, the shelf life of the product. This is the key to making the product stable and usable.
  • This compound chemical fertilizer containing active substances will be applied to farmland, and the microorganisms contained therein must be harmless to humans and animals, have no pollution to the environment, are not potentially dangerous, and can improve the soil and promote the conversion of nutrients. Agricultural beneficial microorganisms that promote the growth of crops.
  • the active agent is prepared by three-stage liquid fermentation or secondary liquid fermentation, first-stage solid fermentation and spore cytochemistry, and the active agent is added as a modifier to the metered chemical fertilizer for compounding. Then, the compounded material is granulated and dried to form a granular composite chemical fertilizer containing active microorganisms.
  • the innovation of the invention is that the compounding of the chemical fertilizer and the microorganism is realized, and the two can coexist in the newly formed composite system, so that the new composite body stably contains the biologically active substance, and the amount thereof is sufficient.
  • the growth of crops has a good effect and plays a significant role in the improvement of soil structure.
  • Bacillus subtilis was selected as a production strain for the production of a compound chemical fertilizer containing an active substance. Using the strain as a seed, the amount of microorganisms is expanded to 10-20 per gram by liquid fermentation, and then through or without a solid fermentation production process.
  • the key is to use the sporulation process. After the spores of Bacillus subtilis, a protective spore-forming outer wall is formed, which is sufficient to withstand the high osmotic pressure environment produced by the saturated solution of chemical fertilizer, so that the microorganism acquires chemical fertilizer. The ability to coexist in the same system, which is then made into biologically active Compound chemical fertilizer.
  • the spore-formed cells are used together with the fermentation substrate as an active agent to facilitate storage, storage and storage, to modify the chemical fertilizer, and to impart biological activity to the modified product.
  • the compound chemical fertilizer containing bioactive substances contains a sufficient amount of chemical nutrients, it can be used like ordinary fertilizers or chemical compound fertilizers, without changing the method of application and application range, Contains 2 grams per gram
  • the dormant spores re-emerge and become nutrient-rich beneficial agricultural microbial cells, which have activated soil, prevent soil compaction, inhibit soil degradation, and restore
  • the good effect of the ground force solves the problem that the common chemical fertilizer or the chemical compound fertilizer destroys the ground force, damages the microorganisms in the soil, and causes the soil performance to deteriorate.
  • a compound chemical fertilizer containing a biologically active substance comprising: a biologically active active agent, an appropriate amount of an organic filler, and a chemical fertilizer having a total active ingredient equal to or greater than 25%.
  • the organic filler is a solid filler, such as weathered coal or agricultural waste such as grass charcoal, grass powder, bagasse.
  • a compound chemical fertilizer containing a biologically active substance which is a microorganism capable of stably surviving in a high osmotic pressure environment formed by a chemical fertilizer, and particularly refers to a state-approved agricultural beneficial microorganism Bacillus subtilis in a dormant state, Bacillus Su lis.
  • Bacillus subtilis deposited on CGMCC on June 15, 2007, with accession number CGMCC2091.
  • the microorganism to which the present invention is added may be a state-approved agricultural beneficial microorganism of the dormant state Bacillus, Bacillus Subtilis.
  • the specific approach is through access to the Chinese number Acccll089 and
  • Bacillus subtilis or a dormant substance obtained by expanding the culture and spore cell process by selecting the agricultural beneficial microorganism Bacillus subtilis, which is identified by other countries.
  • ferment 8-16 ⁇ under the condition of pH 7, until the number of microorganisms reaches 500 million per ml, stop the primary fermentation and transfer the fermentation broth into the secondary fermenter, the capacity ratio of the secondary fermenter
  • the first-stage fermenter is 10-15 times larger, and 10-20 hours of fermentation is carried out under the same conditions as the primary fermentation, and the amount of microorganisms in the secondary fermentation liquid reaches 500 million per milliliter, and the secondary fermentation is stopped;
  • the substrate used here is pulverized agricultural waste such as peat, grass powder, rice husk powder, bagasse, etc., that is, organic filler.
  • the compound chemical fertilizer containing bioactive substances, the expanded culture is a three-stage liquid fermentation, and the third-stage liquid fermentation refers to: first-stage fermentation: inoculating the strain to inoculate In a primary fermentation tank of conventional medium, at a temperature of 30-3
  • the first-stage fermenter is 10-15 times larger, fermenting 10-20 hours under the same conditions as the first-stage fermentation, and the amount of microorganisms in the secondary fermentation liquid reaches 500 million per milliliter, stopping the secondary fermentation, and the second fermentation
  • the resulting product is transferred to a larger than the secondary fermenter 10-
  • the culture is carried out for 20-30 hours under the same conditions as the secondary fermentation, and the number of bacteria to be fermented is 10-20 billion per gram, and the fermentation is stopped.
  • the composite chemical fertilizer containing a biologically active substance according to the above embodiment, wherein the sporulation refers to: raising or adjusting the pH value, deteriorating the ventilation condition, and allowing the natural temperature to rise, the three ways All in order to change the living environment of microorganisms from the most suitable environment for solid fermentation to an unsuitable environment, and to stimulate the transformation of microorganisms into spores in a harsh environment.
  • the spore-forming Bacillus subtilis forms an ellipse and a wall in the cells. Endospore with high water content and strong resistance.
  • the inferior ventilation condition for solid fermentation, it is the frequency of simmering or mixing, stopping the mixing, the degree of violentness of the material, and stimulating the microbial spore by worsening the respiratory conditions of the microorganisms.
  • the mixture is turned over once every half hour and reduced to 1.5-2 hours, and the ambient temperature can be completely stopped.
  • the stirring is stopped, and the supply of gas to the third-stage tank is stopped;
  • the natural elevated temperature is as follows: The temperature of the material is gradually increased from 25-30 c to 50-65 c, and the microbial spores are stimulated by the deterioration of the temperature conditions for the survival of the microorganisms.
  • the heat of the heating process comes from the microbial spores.
  • the heat released, the temperature of the heating process is controlled by the frequency of the mixing, and the rate of turning the mixture is getting faster and faster.
  • the sporulation process is about 10-15 hours, and when 70-90% of the total amount of microorganisms is converted into spores, it is regarded as the end point of the process of sporulation.
  • the treated spores can tolerate more than 1 hour in 100 c water, and the osmotic pressure can be as high as 2 x 106 Pa.
  • the ability to resist acid, alkali, radiation and chemical resistance is also very strong. Under the conditions, it can maintain vitality for several years, even decades.
  • the obtained spores will not be harmed by the high salt index of the fertilizer in the process of blending with the fertilizer in the next step.
  • the activity of microorganisms can also be preserved for a long time.
  • the number of test bacteria is up to 5 per ml
  • the product is transferred to the secondary fermenter.
  • the medium used in the secondary fermenter and the process control conditions are the same as the first-stage fermentation. After the second-stage fermentation is completed, 0.5 kg of gas pressure is added to the tank.
  • the substrate is mainly peat, and is equipped with 1% white sugar and 5% rapeseed cake. Mix thoroughly with a turner, then ferment 48-56 hours at 30 c. During this time, continuously adjust and adjust the pH with lye to keep it at around 7, and keep the temperature of the material at 30 c by turning the heat. Left and right, the number of tested bacteria reached more than 1 billion cockroaches per gram, and the solid fermentation was completed. Stop mixing and adjust the pH to 6.5
  • the value is getting more and more turbulent, so that the microorganisms are stimulated by acidic conditions; due to the stop of the violent gas, the oxygen in the material is less and less; the heat released by the microbial activities cannot be dissipated, the temperature of the material is getting higher and higher, and the water is getting less and less.
  • the weathered coal is evenly mixed, and is granulated, dried, cooled, and packaged to form a chemical compound fertilizer containing biologically active substances.
  • the types of nitrogen, phosphorus, and potassium fertilizers used, and the ratio between them are adjustable, such as 20% urea, 25% diammonium phosphate, 15% potassium chloride or 30%. Urea, 15% monoammonium phosphate, 12%
  • the total amount of NPK fertilizer is also adjustable. By adjusting the total amount of fertilizer and the corresponding amount of organic filler, the total nutrient content can be produced 25%, 30%, 35%.
  • Different fertilizers Different formulas can meet the needs of different crops, and can be adjusted according to the results of soil testing formula.
  • the weathered coal can be replaced by agricultural residues such as peat, bagasse, grass powder and peanut shell.
  • Different organic fillers will make The performance of the product is slightly different, and it does not have a significant impact on product performance. It can be selected according to local conditions.
  • the product is transferred to the secondary fermenter.
  • the medium used in the secondary fermenter and the process control conditions are the same as the first-stage fermentation. After the second-stage fermentation is completed, 0.5 kg of gas pressure is added to the tank.
  • the third-stage fermenter of ° c under the same conditions as the previous one, ferment 12-20 ⁇ , check the number of bacteria to reach more than 1 billion ⁇ per gram, stop stirring the gas supply, adjust the pH value to 6.5. Close the cooling water of the outer jacket of the tank, so that the temperature inside the tank rises continuously, when the temperature rises to 50.
  • the above spores can be sprayed evenly onto a carrier such as peat charcoal, and after the carrier adsorbs the bacterial solution, it becomes an activator having the same performance as the active agent prepared by the solid fermentation method, and is discharged for use. Or wait for Bacillus subtilis 70%
  • the above spore mash was evaporated to a solid under reduced pressure, and mixed with the above amount of the carrier to prepare an activator.
  • Weathered coal is evenly mixed, granulated, dried, cooled, packaged to produce bioactive substances Compound fertilizer.
  • the new fertilizer produced by the above steps is called a compound fertilizer fertilizer containing bioactive substances, referred to as active compound fertilizer.
  • the chemical fertilizer used in the processing is a common chemical fertilizer containing only nitrogen, such as urea, ammonium nitrate, ammonium hydrogencarbonate, etc.
  • the obtained product becomes a nitrogen fertilizer containing an active substance, and likewise, if all the chemicals in the process If the fertilizer is ordinary phosphate fertilizer or potash fertilizer, the obtained products will become phosphate or potash fertilizer containing active substances.
  • Microorganisms or other biological materials related to preservation are Microorganisms or other biological materials related to preservation

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Soil Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Biochemistry (AREA)
  • Fertilizers (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un engrais chimique composite composé de matières bioactives, qui apporte une solution aux problèmes classiques des engrais chimiques, à savoir l'élimination des microbes dans le sol, la perturbation de l'écosystème du sol et le fait de rendre le sol étanche. Le présent engrais se compose d'un agent d'activation contenant des microorganismes viables, des charges organiques et des engrais chimiques. L'engrais contient une quantité de microorganismes supérieure à 2x107 cfu/gramme, une teneur totale en nutriments supérieure ou égale à 25 % et une durée de conservation supérieure à 1 an. L'engrais peut fournir suffisamment de nutriments aux cultures, profiter au sol et prévenir la régression de la productivité du sol. L'invention porte également sur la sélection de la souche bactérienne, les conditions de traitement relatives à la production de l'agent d'activation et le procédé de fabrication de l'engrais chimique composite.
PCT/CN2007/070228 2007-07-04 2007-07-04 Engrais chimique composite comprenant des matériaux bioactifs WO2009003341A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/CN2007/070228 WO2009003341A1 (fr) 2007-07-04 2007-07-04 Engrais chimique composite comprenant des matériaux bioactifs

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2007/070228 WO2009003341A1 (fr) 2007-07-04 2007-07-04 Engrais chimique composite comprenant des matériaux bioactifs

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WO2009003341A1 true WO2009003341A1 (fr) 2009-01-08

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101913961A (zh) * 2010-08-12 2010-12-15 新疆天枣源龟兹生物技术有限责任公司 一种风化煤生物有机肥及其生产方法
CN104876667A (zh) * 2015-05-14 2015-09-02 洛阳茁壮肥业有限公司 一种用于玉米种植的复合肥
CN106966828A (zh) * 2017-03-28 2017-07-21 赵和红 一种液体苗肥
CN108046906A (zh) * 2017-12-18 2018-05-18 天长市天兴园林绿化工程有限公司 一种园林观赏柳苗培育用营养土
CN112094143A (zh) * 2019-05-28 2020-12-18 长春市农业机械研究院 一种高效率、低耗能畜禽粪便转化有机肥设备的发酵工艺
CN116462558A (zh) * 2023-05-10 2023-07-21 国网福建省电力有限公司电力科学研究院 一种塔基植被修复专用复合微生物肥料及其制备方法

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CN1088563A (zh) * 1992-12-25 1994-06-29 郭城 高效生物有机复合肥的生产方法
CN1139092A (zh) * 1996-05-15 1997-01-01 祁弘 微生物肥料浓缩菌种粉及其制造方法
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CN1348939A (zh) * 2000-10-18 2002-05-15 上海大洋生态有机肥有限公司 一种生态有机肥及其制备方法
CN1629104A (zh) * 2003-12-15 2005-06-22 北京全新绿洲国土治理科学研究院 土壤环境生物修复肥料
US20050138978A1 (en) * 2001-12-31 2005-06-30 Microbes, Inc. Fertilizer compositions and methods of making and using same
CN1762917A (zh) * 2005-09-01 2006-04-26 孙明坤 一种微生物有机肥及其制备方法

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1088563A (zh) * 1992-12-25 1994-06-29 郭城 高效生物有机复合肥的生产方法
CN1139092A (zh) * 1996-05-15 1997-01-01 祁弘 微生物肥料浓缩菌种粉及其制造方法
CN1165127A (zh) * 1996-12-13 1997-11-19 中国科学技术大学研究生院 多菌生物有机无机复合颗粒肥料生产方法
CN1348939A (zh) * 2000-10-18 2002-05-15 上海大洋生态有机肥有限公司 一种生态有机肥及其制备方法
US20050138978A1 (en) * 2001-12-31 2005-06-30 Microbes, Inc. Fertilizer compositions and methods of making and using same
CN1629104A (zh) * 2003-12-15 2005-06-22 北京全新绿洲国土治理科学研究院 土壤环境生物修复肥料
CN1762917A (zh) * 2005-09-01 2006-04-26 孙明坤 一种微生物有机肥及其制备方法

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101913961A (zh) * 2010-08-12 2010-12-15 新疆天枣源龟兹生物技术有限责任公司 一种风化煤生物有机肥及其生产方法
CN104876667A (zh) * 2015-05-14 2015-09-02 洛阳茁壮肥业有限公司 一种用于玉米种植的复合肥
CN106966828A (zh) * 2017-03-28 2017-07-21 赵和红 一种液体苗肥
CN108046906A (zh) * 2017-12-18 2018-05-18 天长市天兴园林绿化工程有限公司 一种园林观赏柳苗培育用营养土
CN112094143A (zh) * 2019-05-28 2020-12-18 长春市农业机械研究院 一种高效率、低耗能畜禽粪便转化有机肥设备的发酵工艺
CN116462558A (zh) * 2023-05-10 2023-07-21 国网福建省电力有限公司电力科学研究院 一种塔基植被修复专用复合微生物肥料及其制备方法

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