WO2008156172A1 - 子宮癌、乳癌、及び膀胱癌の予防乃至治療に好適な二本鎖核酸分子、癌細胞増殖抑制剤、並びに医薬 - Google Patents

子宮癌、乳癌、及び膀胱癌の予防乃至治療に好適な二本鎖核酸分子、癌細胞増殖抑制剤、並びに医薬 Download PDF

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Publication number
WO2008156172A1
WO2008156172A1 PCT/JP2008/061346 JP2008061346W WO2008156172A1 WO 2008156172 A1 WO2008156172 A1 WO 2008156172A1 JP 2008061346 W JP2008061346 W JP 2008061346W WO 2008156172 A1 WO2008156172 A1 WO 2008156172A1
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WIPO (PCT)
Prior art keywords
cancer
cancer cell
double
nucleic acid
acid molecule
Prior art date
Application number
PCT/JP2008/061346
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English (en)
French (fr)
Inventor
Satoshi Inoue
Kazuhiro Ikeda
Original Assignee
Saitama Medical University
Rnai Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Saitama Medical University, Rnai Co., Ltd. filed Critical Saitama Medical University
Priority to JP2009520560A priority Critical patent/JP5335673B2/ja
Priority to EP08777490.7A priority patent/EP2184352B1/en
Publication of WO2008156172A1 publication Critical patent/WO2008156172A1/ja
Priority to US12/642,979 priority patent/US8124754B2/en

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/713—Double-stranded nucleic acids or oligonucleotides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Biomedical Technology (AREA)
  • Epidemiology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

　本発明は、エストロゲン応答遺伝子であるＣＯＸ７ＲＰ遺伝子及びＥｆｐ遺伝子の少なくともいずれかを標的とし、これらの遺伝子の発現を抑制することにより、子宮癌、乳癌、及び膀胱癌の癌細胞の増殖を効果的に抑制することが可能なｓｉＲＮＡ等の二本鎖核酸分子、並びに癌細胞増殖抑制剤及び医薬を提供することを目的とする。即ち本発明は、（ａ）配列番号：１～配列番号：３８のいずれかの標的配列に対応するヌクレオチド配列を有するセンス鎖と、（ｂ）前記（ａ）のセンス鎖に相補的なヌクレオチド配列を有するアンチセンス鎖とを含む、ＣＯＸ７ＲＰ遺伝子及びＥｆｐ遺伝子の少なくともいずれかの発現を抑制するための二本鎖核酸分子；前記二本鎖核酸分子を含む子宮癌細胞、乳癌細胞、及び膀胱癌細胞の少なくともいずれかに対する癌細胞増殖抑制剤；並びに、前記癌細胞増殖抑制剤を含む子宮癌、乳癌、及び膀胱癌の少なくともいずれかに対する医薬に関する。
PCT/JP2008/061346 2007-06-20 2008-06-20 子宮癌、乳癌、及び膀胱癌の予防乃至治療に好適な二本鎖核酸分子、癌細胞増殖抑制剤、並びに医薬 WO2008156172A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2009520560A JP5335673B2 (ja) 2007-06-20 2008-06-20 子宮癌、乳癌、及び膀胱癌の予防乃至治療に好適な二本鎖核酸分子、癌細胞増殖抑制剤、並びに医薬
EP08777490.7A EP2184352B1 (en) 2007-06-20 2008-06-20 Double-stranded nucleic acid molecule, cancer cell proliferation inhibitor and pharmaceutical agent suitable for prevention or treatment of uterine cancer, breast cancer and bladder cancer
US12/642,979 US8124754B2 (en) 2007-06-20 2009-12-21 Double-stranded nucleic acid molecule cancer cell proliferation inhibitor and pharmaceutical agent suitable for prevention or treatment of uterine cancer, breast cancer, and bladder cancer

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007162641 2007-06-20
JP2007-162641 2007-06-20

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/642,979 Continuation US8124754B2 (en) 2007-06-20 2009-12-21 Double-stranded nucleic acid molecule cancer cell proliferation inhibitor and pharmaceutical agent suitable for prevention or treatment of uterine cancer, breast cancer, and bladder cancer

Publications (1)

Publication Number Publication Date
WO2008156172A1 true WO2008156172A1 (ja) 2008-12-24

Family

ID=40156331

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/061346 WO2008156172A1 (ja) 2007-06-20 2008-06-20 子宮癌、乳癌、及び膀胱癌の予防乃至治療に好適な二本鎖核酸分子、癌細胞増殖抑制剤、並びに医薬

Country Status (4)

Country Link
US (1) US8124754B2 (ja)
EP (1) EP2184352B1 (ja)
JP (1) JP5335673B2 (ja)
WO (1) WO2008156172A1 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011087009A1 (ja) * 2010-01-14 2011-07-21 学校法人埼玉医科大学 糖代謝、脂質代謝、肥満、及び寿命を制御する制御遺伝子、及びタンパク質、並びにスクリーニング方法
CN104109670A (zh) * 2014-03-12 2014-10-22 首都医科大学附属北京安定医院 一种双链siRNA分子及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3803318B2 (ja) * 2001-11-21 2006-08-02 株式会社ＲＮＡｉ 遺伝子発現阻害方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2467936C (en) * 2001-11-21 2013-11-05 Mitsubishi Chemical Corporation Method of inhibiting gene expression
US20040110159A1 (en) * 2002-12-10 2004-06-10 Isis Pharmaceuticals Inc. Modulation of estrogen-responsive finger protein expression
WO2003102185A2 (en) * 2002-05-30 2003-12-11 Telogene Inc. Methods and compositions for treating neoplasia relating to hnrnp a1 and a2 nucleic acid molecules
GB0317466D0 (en) 2003-07-25 2003-08-27 Univ Sheffield Use

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3803318B2 (ja) * 2001-11-21 2006-08-02 株式会社ＲＮＡｉ 遺伝子発現阻害方法

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
HOBO-HAYASHI R.: "Expression and regulation of an estrogen-responsive gene, cytochrome c oxidase subunit 7a related polypeptide (COX7RP) in endometrial cancer", J. SAITAMA MED. SCHOOL, vol. 31, 2004, pages 199 - 206 *
See also references of EP2184352A4 *
URANO T. ET AL.: "Efp targets 14-3-3-sigma for proteolysis and promotes breast tumour growth", NATURE, vol. 417, 2002, pages 871 - 875, XP008129066 *
WATANABE T. ET AL.: "Isolation of estrogen-responsive genes with a CpG island library", MOL. CELL BIOL., vol. 18, no. 1, 1998, pages 442 - 449, XP008129068 *
ZOU W. AND ZHANG D.-E.: "The interferon-inducible ubiquitin-protein isopeptide ligase (E3) EFP also functions as an ISG15 E3 ligase", J. BIOL. CHEM., vol. 281, no. 7, 2006, pages 3989 - 3994, XP008129067 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011087009A1 (ja) * 2010-01-14 2011-07-21 学校法人埼玉医科大学 糖代謝、脂質代謝、肥満、及び寿命を制御する制御遺伝子、及びタンパク質、並びにスクリーニング方法
JP5823299B2 (ja) * 2010-01-14 2015-11-25 学校法人 埼玉医科大学 糖代謝、脂質代謝、肥満、及び寿命の少なくともいずれかを制御する作用を有する物質のインビトロでのスクリーニング方法
CN104109670A (zh) * 2014-03-12 2014-10-22 首都医科大学附属北京安定医院 一种双链siRNA分子及其应用

Also Published As

Publication number Publication date
US20100173405A1 (en) 2010-07-08
EP2184352B1 (en) 2015-09-09
JPWO2008156172A1 (ja) 2010-08-26
JP5335673B2 (ja) 2013-11-06
US8124754B2 (en) 2012-02-28
EP2184352A1 (en) 2010-05-12
EP2184352A4 (en) 2012-08-08

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