WO2008138341A3 - Methods for detection of 'pontiac' subgroups of legionella pneumophila serogroup 1 - Google Patents

Methods for detection of 'pontiac' subgroups of legionella pneumophila serogroup 1 Download PDF

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Publication number
WO2008138341A3
WO2008138341A3 PCT/DK2008/000177 DK2008000177W WO2008138341A3 WO 2008138341 A3 WO2008138341 A3 WO 2008138341A3 DK 2008000177 W DK2008000177 W DK 2008000177W WO 2008138341 A3 WO2008138341 A3 WO 2008138341A3
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WO
WIPO (PCT)
Prior art keywords
pontiac
lpn
subgroups
gene
detection
Prior art date
Application number
PCT/DK2008/000177
Other languages
French (fr)
Other versions
WO2008138341A2 (en
Inventor
Soren Uldum
Original Assignee
Statens Seruminstitut
Soren Uldum
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Statens Seruminstitut, Soren Uldum filed Critical Statens Seruminstitut
Publication of WO2008138341A2 publication Critical patent/WO2008138341A2/en
Publication of WO2008138341A3 publication Critical patent/WO2008138341A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Methods based on serogroup specific DNA sequences and subgroup- specific gene of Lpn serogroup (sg) 1 strains for detection and discrimination of all monoclonal Lpn sg 1 Pontiac and non-Pontiac subgroups in clinical and environmental samples are described. Primers were designed for the Lipopolysaccharide (LPS) associated (lag-1) gene, which codes for a common LPS epitope specific for the MAb 3/1 of the Dresden monoclonal panel (sg 1 'Pontiac' subgroups), and primers for open reading frame 2 (ORF 2) DNA sequence of the LPS biosynthesis gene cluster of Lpn sg 1. PCR with primers to the lag-l gene and ORF 2 can be used for diagnosis of LD caused by Lpn sg 1 without need for isolation by culture. The PCR method can be used as a rapid method for detection and discrimination between the Pontiac and non-Pontiac subgroups of Lpn sg 1 in clinical and environmental samples before culture and serogroup results can be obtained. The PCR and DNA methods based on lag-1 gene and ORF 2 DNA sequences could be a valuable tool in outbreak investigations and in risk assessment.
PCT/DK2008/000177 2007-05-11 2008-05-09 Methods for detection of 'pontiac' subgroups of legionella pneumophila serogroup 1 WO2008138341A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200700712 2007-05-11
DKPA200700712 2007-05-11

Publications (2)

Publication Number Publication Date
WO2008138341A2 WO2008138341A2 (en) 2008-11-20
WO2008138341A3 true WO2008138341A3 (en) 2009-01-08

Family

ID=39870394

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2008/000177 WO2008138341A2 (en) 2007-05-11 2008-05-09 Methods for detection of 'pontiac' subgroups of legionella pneumophila serogroup 1

Country Status (1)

Country Link
WO (1) WO2008138341A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2949788A1 (en) * 2009-08-21 2011-03-11 Pasteur Institut SPECIFIC MARKER AND METHOD FOR THE DETECTION AND IDENTIFICATION OF BACTERIUM LEGIONELLA PNEUMOPHILA SEROGROUPE 1

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001042791A1 (en) * 1999-12-10 2001-06-14 Binax, Inc. Eia for monitoring legionella pneumophila presence in water samples

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001042791A1 (en) * 1999-12-10 2001-06-14 Binax, Inc. Eia for monitoring legionella pneumophila presence in water samples

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
"59. Jahrestagung der Deutschen Gesellschaft fuer Hygiene und Mikrobiologie", INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, URBAN UND FISCHER, DE, vol. 297, 29 August 2007 (2007-08-29), pages 1 - 173, XP022218177, ISSN: 1438-4221 *
DATABASE EMBL April 2005 (2005-04-01), LÜNEBERG E., XP002502181, retrieved from NCBI Database accession no. AJ007311 *
HELBIG J H ET AL: "Diagnostic relevance of the detection of Legionella DNA in urine samples by the polymerase chain reaction", EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY AND INFECTIOUS DISEASES, vol. 18, no. 10, October 1999 (1999-10-01), pages 716 - 722, XP002502180, ISSN: 0934-9723 *
LÜCK P C ET AL: "Pathogenesis, diagnosis and therapy of Legionella infections", BUNDESGESUNDHEITSBLATT GESUNDHEITSFORSCH.GESUNDHEITSSCHUTZ, SPRINGER, BERLIN, DE, vol. 49, no. 5, 1 May 2006 (2006-05-01), pages 439 - 449, XP019389140, ISSN: 1437-1588 *
LUENEBERG EDELTRAUD ET AL: "Cloning and functional characterization of a 30 kb gene locus required for lipopolysaccharide biosynthesis in Legionella pneumophila", IJMM INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, vol. 290, no. 1, March 2000 (2000-03-01), pages 37 - 49, XP009108023, ISSN: 1438-4221 *
ZOU C H ET AL: "Molecular cloning and characterization of a locus responsible for O acetylation of the O polysaccharide of Legionella pneumophila serogroup 1 lipopolysaccharide.", JOURNAL OF BACTERIOLOGY JUL 1999, vol. 181, no. 13, July 1999 (1999-07-01), pages 4137 - 4141, XP002502179, ISSN: 0021-9193 *

Also Published As

Publication number Publication date
WO2008138341A2 (en) 2008-11-20

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