WO2006097347A3 - Methods for detecting, identifying and differentiating species and vaccine strains of the brucella genus - Google Patents

Methods for detecting, identifying and differentiating species and vaccine strains of the brucella genus Download PDF

Info

Publication number
WO2006097347A3
WO2006097347A3 PCT/EP2006/002612 EP2006002612W WO2006097347A3 WO 2006097347 A3 WO2006097347 A3 WO 2006097347A3 EP 2006002612 W EP2006002612 W EP 2006002612W WO 2006097347 A3 WO2006097347 A3 WO 2006097347A3
Authority
WO
WIPO (PCT)
Prior art keywords
identifying
detecting
vaccine strains
methods
differentiating
Prior art date
Application number
PCT/EP2006/002612
Other languages
French (fr)
Other versions
WO2006097347A2 (en
Inventor
Ignacio Lopez-Goni
Yoldi David Garcia
Original Assignee
Inst Cientifico Tecnol Navarra
Ignacio Lopez-Goni
Yoldi David Garcia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Cientifico Tecnol Navarra, Ignacio Lopez-Goni, Yoldi David Garcia filed Critical Inst Cientifico Tecnol Navarra
Publication of WO2006097347A2 publication Critical patent/WO2006097347A2/en
Publication of WO2006097347A3 publication Critical patent/WO2006097347A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Abstract

The method for detecting genus, identifying and differentiating species and vaccine strains of the Brucella is based on the amplification, by means of multiplex PCR, of selected genomic DNA fragments corresponding to genomic regions of interest. Said method allows detecting, differentiating and identifying the strains of B. abortus, B. melitensis, B. ovis, B. suis, B. neotomae, B. canis, the isolates of marine mammal Brucella, and the vaccine strains B. abortus S19 (B19), B. abortus RB51 and B. melitensis Rev 1.
PCT/EP2006/002612 2005-03-17 2006-03-16 Methods for detecting, identifying and differentiating species and vaccine strains of the brucella genus WO2006097347A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
ESP200500629 2005-03-17
ES200500629 2005-03-17
ESP200503083 2005-12-14
ES200503083 2005-12-14

Publications (2)

Publication Number Publication Date
WO2006097347A2 WO2006097347A2 (en) 2006-09-21
WO2006097347A3 true WO2006097347A3 (en) 2007-01-04

Family

ID=36930123

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2006/002612 WO2006097347A2 (en) 2005-03-17 2006-03-16 Methods for detecting, identifying and differentiating species and vaccine strains of the brucella genus

Country Status (1)

Country Link
WO (1) WO2006097347A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MY182940A (en) * 2013-10-07 2021-02-05 Mitsui Chemicals Inc Pcr primer set for bacterial dna amplification, kit for detecting and/or identifying bacterial species, and method for detecting and/or identifying bacterial species
CN105713967B (en) * 2016-01-29 2020-06-16 江汉大学 Qualitative and quantitative detection method for water body microorganisms
RU2621864C1 (en) * 2016-09-19 2017-06-07 Федеральное казенное учреждение здравоохранения "Российский научно-исследовательский противочумный институт "Микроб" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека ("РосНИПЧИ "Микроб") Method for brucellosis pathogen species identification by pcr method with hybridization-fluorescent results accounting in real time
CN108588247A (en) * 2018-05-02 2018-09-28 杭州优思达生物技术有限公司 A kind of kit and its detection method of brucella nucleic acid
CN111733217A (en) * 2019-10-09 2020-10-02 中国疾病预防控制中心传染病预防控制所 Method for detecting brucella nucleic acid DNA by digital PCR
CN111593048B (en) * 2020-05-27 2024-04-19 新疆畜牧科学院兽医研究所（新疆畜牧科学院动物临床医学研究中心） Primer combination, kit, amplification method and application in A19 vaccine detection
CN113186312B (en) * 2021-05-03 2022-10-04 中国动物卫生与流行病学中心 Molecular marker for distinguishing Brucella A19 vaccine strain and wild strain
CN115786541B (en) * 2022-07-14 2023-06-27 天津健博生物科技有限公司 SNP molecular marker, primer probe, kit, method and application for identifying Brucella vaccine strain A19
CN116590436B (en) * 2023-03-15 2024-02-06 中国农业科学院北京畜牧兽医研究所 RT-PCR kit for identifying Brucella vaccine strain Rev.1

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2078174A1 (en) * 1993-12-16 1995-12-01 Univ Cantabria Molecular diagnostic methods for the detection of Brucella abortus and differentiation from vaccine strain B19

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2078174A1 (en) * 1993-12-16 1995-12-01 Univ Cantabria Molecular diagnostic methods for the detection of Brucella abortus and differentiation from vaccine strain B19

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
BRICKER BETSY J ET AL: "Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 32, no. 11, 1994, pages 2660 - 2666, XP002398170, ISSN: 0095-1137 *
BRICKER BETSY J ET AL: "Enhancement of the Brucella AMOS PCR assay for differentiation of Brucella abortus vaccine strains S19 and RB51", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 33, no. 6, 1995, pages 1640 - 1642, XP002398169, ISSN: 0095-1137 *
BRICKER BETSY J: "PCR as a diagnostic tool for brucellosis.", VETERINARY MICROBIOLOGY, vol. 90, no. 1-4, 20 December 2002 (2002-12-20), pages 435 - 446, XP002398173, ISSN: 0378-1135 *
CLOECKAERT A ET AL: "Identification of Brucella melitensis vaccine strain Rev.1 by PCR-RFLP based on a mutation in the rpsL gene", VACCINE, BUTTERWORTH SCIENTIFIC. GUILDFORD, GB, vol. 20, no. 19-20, 7 June 2002 (2002-06-07), pages 2546 - 2550, XP004359686, ISSN: 0264-410X *
FERRAO-BECK LENIA ET AL: "Development of a multiplex PCR assay for polymorphism analysis of Brucella suis biovars causing brucellosis in swine", VETERINARY MICROBIOLOGY, vol. 115, no. 1-3, June 2006 (2006-06-01), pages 269 - 277, XP002398172, ISSN: 0378-1135 *
GARCÍA-YOLDI D ET AL: "Multiplex PCR assay for the identification and differentiation of all Brucella species and the vaccine strains Brucella abortus S19 and RB51 and Brucella melitensis Rev1 [13]", CLINICAL CHEMISTRY 2006 UNITED STATES, vol. 52, no. 4, 2006, pages 779 - 781, XP009072107, ISSN: 0009-9147 *
PROBERT W S ET AL: "Real-Time Multiplex PCR Assay for Detection of Brucella spp., B. abortus, and B. melitensis", JOURNAL OF CLINICAL MICROBIOLOGY 2004 UNITED STATES, vol. 42, no. 3, 2004, pages 1290 - 1293, XP002398167, ISSN: 0095-1137 *
SREEVATSAN S ET AL: "Molecular detection of Brucella abortus and/or Mycobacterium bovis infection in cattle", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 100, 2000, & 100TH GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY; LOS ANGELES, CALIFORNIA, USA; MAY 21-25, 2000, pages 139, XP009072077, ISSN: 1060-2011 *
VEMULAPALLI RAMESH ET AL: "Identification of an IS711 element interrupting the wboA gene of Brucella abortus vaccine strain RB51 and a PCR assay to distinguish strain RB51 from other Brucella species and strains", CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, vol. 6, no. 5, September 1999 (1999-09-01), pages 760 - 764, XP002398166, ISSN: 1071-412X *

Also Published As

Publication number Publication date
WO2006097347A2 (en) 2006-09-21

Similar Documents

Publication Publication Date Title
WO2006097347A3 (en) Methods for detecting, identifying and differentiating species and vaccine strains of the brucella genus
DK1421211T3 (en) Methods for detecting DNA originating from different individuals
WO2009015863A3 (en) Methods of detecting methylated dna at a specific locus
WO2010083250A3 (en) Single-cell nucleic acid analysis
WO2011020011A3 (en) Droplet actuator and droplet-based techniques
EP1362929A3 (en) Methods for genotyping
AP2276A (en) A method for diagnosis of tuberculosis by smear microscopy, culture and polymerase chain reaction using processed clinical samples and kit thereof.
WO2006057918A3 (en) Detection of nucleic acid variation by cleavage-amplification method
WO2006122312A3 (en) Methods of testing using a microfluidic cassette
WO2006108674A3 (en) Elite event a2704-12 and methods and kits for identifying such event in biological samples
WO2009092035A3 (en) Methods and compositions for the analysis of biological molecules
WO2007075836A3 (en) Methods for detection of genetic disorders
WO2008028210A3 (en) Detection of bacteria
WO2011132061A3 (en) Methylation profiling of dna samples
WO2010075414A3 (en) Method for detection of xmrv
WO2006088911A3 (en) Sequence-specific detection of nucleotide sequences
WO2012174119A3 (en) Compositions and methods for detection of cronobacter spp. and cronobacter species and strains
WO2009153667A3 (en) Method for the detection of ovarian cancer
WO2005100611A3 (en) Detection of viral nucleic acid and method for reverse transcribing rna
WO2005054516A3 (en) Peptide nucleic acid probes for analysis of certain staphylococcus species
WO2001068913A3 (en) Nucleic acid detection method and system
WO2008119332A3 (en) Method for the detection of paratuberculosis
WO2002077293A3 (en) Detection of fungal pathogens using the polymerase chain reaction
WO2005074522A3 (en) Detection of ruminant dna via pcr
WO2006103111A3 (en) Improved bisulfite conversion of dna

Legal Events

Date Code Title Description
NENP Non-entry into the national phase

Ref country code: DE

NENP Non-entry into the national phase

Ref country code: RU

WWW Wipo information: withdrawn in national office

Country of ref document: RU

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 06723611

Country of ref document: EP

Kind code of ref document: A2

122 Ep: pct application non-entry in european phase

Ref document number: 06723611

Country of ref document: EP

Kind code of ref document: A2

WWW Wipo information: withdrawn in national office

Ref document number: 6723611

Country of ref document: EP