WO2008126400A1 - タンパク質リフォールディング用カラム充填剤およびカラム - Google Patents

タンパク質リフォールディング用カラム充填剤およびカラム Download PDF

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Publication number
WO2008126400A1
WO2008126400A1 PCT/JP2008/000877 JP2008000877W WO2008126400A1 WO 2008126400 A1 WO2008126400 A1 WO 2008126400A1 JP 2008000877 W JP2008000877 W JP 2008000877W WO 2008126400 A1 WO2008126400 A1 WO 2008126400A1
Authority
WO
WIPO (PCT)
Prior art keywords
column
protein
refolding
packing
column packing
Prior art date
Application number
PCT/JP2008/000877
Other languages
English (en)
French (fr)
Inventor
Masayuki Kawakami
Tatsuo Tsunoda
Hideaki Togashi
Takayuki Nara
Shun-Ichi Matsuura
Chisato Sekikawa
Akiko Kawai
Akiyoshi Kawata
Fujio Mizukami
Kengo Sakaguchi
Original Assignee
Fujifilm Corporation
National Institute Of Advanced Industrial Science And Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujifilm Corporation, National Institute Of Advanced Industrial Science And Technology filed Critical Fujifilm Corporation
Priority to US12/593,713 priority Critical patent/US8394933B2/en
Priority to EP08738489.7A priority patent/EP2141174B1/en
Priority to JP2009508911A priority patent/JP5137038B2/ja
Publication of WO2008126400A1 publication Critical patent/WO2008126400A1/ja

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/52—Physical parameters
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
    • B01J20/10—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising silica or silicate
    • B01J20/16—Alumino-silicates
    • B01J20/18—Synthetic zeolitic molecular sieves
    • B01J20/183—Physical conditioning without chemical treatment, e.g. drying, granulating, coating, irradiation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28014—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their form
    • B01J20/2803—Sorbents comprising a binder, e.g. for forming aggregated, agglomerated or granulated products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
    • B01D15/206—Packing or coating
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/58—Use in a single column
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/52—Physical parameters
    • G01N2030/524—Physical parameters structural properties
    • G01N2030/525—Physical parameters structural properties surface properties, e.g. porosity
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/29—Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
    • Y10T428/2982—Particulate matter [e.g., sphere, flake, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

　本発明の課題は、大腸菌等で生産した高次構造未形成による不活性タンパク質、あるいはある種の原因で立体構造が変化し失活したタンパク質のリフォールディングすなわち機能賦活に対して効果的であるタンパク質リフォールディング用カラム充填剤、並びに該カラム充填剤を充填したカラムを提供することである。本発明によれば、粒子状に造粒されているＢＥＡ構造のゼオライト（ゼオライトベータ）を含む、タンパク質リフォールディング用カラム充填剤が提供される。
PCT/JP2008/000877 2007-04-05 2008-04-04 タンパク質リフォールディング用カラム充填剤およびカラム WO2008126400A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US12/593,713 US8394933B2 (en) 2007-04-05 2008-04-04 Protein refolding column filler and column
EP08738489.7A EP2141174B1 (en) 2007-04-05 2008-04-04 Protein refolding column filler and column
JP2009508911A JP5137038B2 (ja) 2007-04-05 2008-04-04 タンパク質リフォールディング用カラム充填剤およびカラム

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007099284 2007-04-05
JP2007-099284 2007-04-05

Publications (1)

Publication Number Publication Date
WO2008126400A1 true WO2008126400A1 (ja) 2008-10-23

Family

ID=39863560

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/000877 WO2008126400A1 (ja) 2007-04-05 2008-04-04 タンパク質リフォールディング用カラム充填剤およびカラム

Country Status (4)

Country Link
US (1) US8394933B2 (ja)
EP (1) EP2141174B1 (ja)
JP (1) JP5137038B2 (ja)
WO (1) WO2008126400A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011046686A (ja) * 2009-06-10 2011-03-10 National Institute Of Advanced Industrial Science & Technology タンパク質リフォールディング条件設定キット

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004330113A (ja) * 2003-05-08 2004-11-25 Pentax Corp 吸着剤の製造方法および吸着剤
WO2005005459A1 (ja) * 2003-07-09 2005-01-20 National Institute Of Advanced Industrial Science And Technology タンパク質巻き戻し材料
JP2005029531A (ja) 2003-07-09 2005-02-03 National Institute Of Advanced Industrial & Technology 不活性タンパク質の機能賦活方法
JP2005192452A (ja) 2004-01-05 2005-07-21 National Institute Of Advanced Industrial & Technology タンパク質巻き戻し用キット
JP2005220121A (ja) 2004-01-05 2005-08-18 National Institute Of Advanced Industrial & Technology タンパク質巻き戻し材料
JP2005281267A (ja) 2004-03-31 2005-10-13 National Institute Of Advanced Industrial & Technology タンパク質巻き戻し成形体

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2071962C (en) * 1990-02-12 1994-09-20 Nancy Karapasha High capacity odor controlling compositions
JPH08508423A (ja) * 1993-03-31 1996-09-10 ザ、プロクター、エンド、ギャンブル、カンパニー ポジティブな香気シグナルを有する臭気抑制用の吸収物品
US6402958B1 (en) * 2000-08-17 2002-06-11 Uop Llc Chromatography column loading method
US7417003B2 (en) * 2006-12-29 2008-08-26 Uop Llc Solid acid catalyst and process for decomposition of cumene hydroperoxide

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004330113A (ja) * 2003-05-08 2004-11-25 Pentax Corp 吸着剤の製造方法および吸着剤
WO2005005459A1 (ja) * 2003-07-09 2005-01-20 National Institute Of Advanced Industrial Science And Technology タンパク質巻き戻し材料
JP2005029531A (ja) 2003-07-09 2005-02-03 National Institute Of Advanced Industrial & Technology 不活性タンパク質の機能賦活方法
JP2005192452A (ja) 2004-01-05 2005-07-21 National Institute Of Advanced Industrial & Technology タンパク質巻き戻し用キット
JP2005220121A (ja) 2004-01-05 2005-08-18 National Institute Of Advanced Industrial & Technology タンパク質巻き戻し材料
JP2005281267A (ja) 2004-03-31 2005-10-13 National Institute Of Advanced Industrial & Technology タンパク質巻き戻し成形体

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ANAL. BIOCHEM., vol. 348, 2006, pages 307 - 314
CHEM. EUR. J., vol. 7, 2001, pages 1555 - 1560
CHIKU H. ET AL.: "A novel protein refolding method using a zeolite", ANAL. BIOCHEM., vol. 348, no. 2, 2006, pages 307 - 314, XP005217382 *
HYOMEN, SURFACES, vol. 37, 1999, pages 537 - 557
STUD. SURF. SCI. CATAL., vol. 125, 1999, pages 1 - 12
ZEOLITES, vol. 11, 1991, pages 202

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011046686A (ja) * 2009-06-10 2011-03-10 National Institute Of Advanced Industrial Science & Technology タンパク質リフォールディング条件設定キット

Also Published As

Publication number Publication date
JPWO2008126400A1 (ja) 2010-07-22
US20100210820A1 (en) 2010-08-19
US8394933B2 (en) 2013-03-12
EP2141174B1 (en) 2018-05-30
JP5137038B2 (ja) 2013-02-06
EP2141174A1 (en) 2010-01-06
EP2141174A4 (en) 2010-08-11

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