WO2008115844A3 - Holoenzymes de cytochrome p50 chimérique fonctionnelles et stables - Google Patents

Holoenzymes de cytochrome p50 chimérique fonctionnelles et stables Download PDF

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Publication number
WO2008115844A3
WO2008115844A3 PCT/US2008/057174 US2008057174W WO2008115844A3 WO 2008115844 A3 WO2008115844 A3 WO 2008115844A3 US 2008057174 W US2008057174 W US 2008057174W WO 2008115844 A3 WO2008115844 A3 WO 2008115844A3
Authority
WO
WIPO (PCT)
Prior art keywords
holoenzymes
stable
functional chimeric
cytochrome
chimeric cytochrome
Prior art date
Application number
PCT/US2008/057174
Other languages
English (en)
Other versions
WO2008115844A2 (fr
Inventor
Frances H Arnold
Yougen Li
Original Assignee
California Inst Of Techn
Frances H Arnold
Yougen Li
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US12/024,515 external-priority patent/US20080248545A1/en
Application filed by California Inst Of Techn, Frances H Arnold, Yougen Li filed Critical California Inst Of Techn
Publication of WO2008115844A2 publication Critical patent/WO2008115844A2/fr
Publication of WO2008115844A3 publication Critical patent/WO2008115844A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La présente invention concerne des polypeptides de fusion de cytochrome 450, des acides nucléiques codant les polypeptides et des cellules hôtes pour produire les polypeptides.
PCT/US2008/057174 2007-03-16 2008-03-15 Holoenzymes de cytochrome p50 chimérique fonctionnelles et stables WO2008115844A2 (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US91852807P 2007-03-16 2007-03-16
US60/918,528 2007-03-16
US12/024,515 US20080248545A1 (en) 2007-02-02 2008-02-01 Methods for Generating Novel Stabilized Proteins
US12/024,515 2008-02-01
US2788508A 2008-02-07 2008-02-07
US12/027,885 2008-02-07

Publications (2)

Publication Number Publication Date
WO2008115844A2 WO2008115844A2 (fr) 2008-09-25
WO2008115844A3 true WO2008115844A3 (fr) 2009-05-14

Family

ID=39766722

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/057174 WO2008115844A2 (fr) 2007-03-16 2008-03-15 Holoenzymes de cytochrome p50 chimérique fonctionnelles et stables

Country Status (1)

Country Link
WO (1) WO2008115844A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7524664B2 (en) 2003-06-17 2009-04-28 California Institute Of Technology Regio- and enantioselective alkane hydroxylation with modified cytochrome P450
US8252559B2 (en) 2006-08-04 2012-08-28 The California Institute Of Technology Methods and systems for selective fluorination of organic molecules
US8026085B2 (en) 2006-08-04 2011-09-27 California Institute Of Technology Methods and systems for selective fluorination of organic molecules
US8802401B2 (en) 2007-06-18 2014-08-12 The California Institute Of Technology Methods and compositions for preparation of selectively protected carbohydrates
EP2426198A1 (fr) 2010-09-03 2012-03-07 B.R.A.I.N. Biotechnology Research And Information Network AG Variantes de cytochrome P450 monooxygénase
US9322007B2 (en) 2011-07-22 2016-04-26 The California Institute Of Technology Stable fungal Cel6 enzyme variants
CN116790529A (zh) * 2023-05-24 2023-09-22 天津大学 P450 bm3蛋白突变体及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005017106A2 (fr) * 2003-06-17 2005-02-24 California Institute Of Technology Bibliotheques d'enzymes cytochromes p450 optimisees et enzymes p450 optimisees

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005017106A2 (fr) * 2003-06-17 2005-02-24 California Institute Of Technology Bibliotheques d'enzymes cytochromes p450 optimisees et enzymes p450 optimisees

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
LANDWEHR ET AL: "Diversification of Catalytic Function in a Synthetic Family of Chimeric Cytochrome P450s", CHEMISTRY AND BIOLOGY, CURRENT BIOLOGY, LONDON, GB, vol. 14, no. 3, 23 March 2007 (2007-03-23), pages 269 - 278, XP005929291, ISSN: 1074-5521 *
MEYER MICHELLE M ET AL: "Library analysis of SCHEMA-guided protein recombination.", PROTEIN SCIENCE, vol. 12, no. 8, August 2003 (2003-08-01), pages 1686 - 1693, XP002502197, ISSN: 0961-8368 *
OTEY CHRISTOPHER R ET AL: "Structure-guided recombination creates an artificial family of cytochromes P450", PLOS BIOLOGY, vol. 4, no. 5, May 2006 (2006-05-01), pages 789 - 798, XP002502196, ISSN: 1544-9173(print) 1545-7885(ele *

Also Published As

Publication number Publication date
WO2008115844A2 (fr) 2008-09-25

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