WO2008111599A1 - 生体関連物質の検出方法 - Google Patents

生体関連物質の検出方法 Download PDF

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Publication number
WO2008111599A1
WO2008111599A1 PCT/JP2008/054446 JP2008054446W WO2008111599A1 WO 2008111599 A1 WO2008111599 A1 WO 2008111599A1 JP 2008054446 W JP2008054446 W JP 2008054446W WO 2008111599 A1 WO2008111599 A1 WO 2008111599A1
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WO
WIPO (PCT)
Prior art keywords
biologically relevant
relevant substance
binding
detecting
immobilizing
Prior art date
Application number
PCT/JP2008/054446
Other languages
English (en)
French (fr)
Inventor
Masaaki Miyaji
Mitsuhiro Murata
Toshihiro Ogawa
Kouji Tamori
Eiji Takamoto
Original Assignee
Jsr Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jsr Corporation filed Critical Jsr Corporation
Publication of WO2008111599A1 publication Critical patent/WO2008111599A1/ja

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Nanotechnology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

　本発明は、特定の生体関連物質と特異的に相互作用するプローブを粒子に固定したアフィニティー担体を用い、その生体関連物質を高感度で検出する方法を提供することを目的とする。本発明に係る生体関連物質の検出方法は、トシル化（ｐ－トルエンスルホニル化）または多価カルボン酸無水物によりエステル化された２，３－ジヒドロキシプロピル基を有する数平均粒子径２～３０μｍの有機ポリマー粒子に、検出の対象とする生体関連物質と特異的に結合する一次プローブが固定化して得られた第一の粒子と、数平均粒子径２～２０ｎｍの半導体ナノ粒子に、前記生体関連物質に結合可能な二次プローブを固定化して得られた第二の粒子とを、前記生体関連物質を介して結合させる工程を含むことを特徴とする。
PCT/JP2008/054446 2007-03-12 2008-03-12 生体関連物質の検出方法 WO2008111599A1 (ja)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2007061950 2007-03-12
JP2007-061950 2007-03-12
JP2007-079085 2007-03-26
JP2007079085 2007-03-26

Publications (1)

Publication Number Publication Date
WO2008111599A1 true WO2008111599A1 (ja) 2008-09-18

Family

ID=39759533

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/054446 WO2008111599A1 (ja) 2007-03-12 2008-03-12 生体関連物質の検出方法

Country Status (1)

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WO (1) WO2008111599A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019511707A (ja) * 2016-02-18 2019-04-25 オプトフルイディクス インコーポレイテッド 流体試料中の粒子を特徴付けるためのシステム及び方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57135801A (en) * 1980-12-23 1982-08-21 Boehringer Mannheim Gmbh Hydrophilic latex particles, manufacture and diagnostic drug containing same
JP2003322654A (ja) * 2002-02-27 2003-11-14 Hitachi Software Eng Co Ltd 生体高分子の検出方法
JP2005148048A (ja) * 2003-04-25 2005-06-09 Jsr Corp バイオチップおよびバイオチップキットならびにその製造方法および使用方法
JP2007211076A (ja) * 2006-02-08 2007-08-23 Jsr Corp 有機ポリマー粒子およびその製造方法、ならびにプローブ結合粒子
JP2007288133A (ja) * 2006-03-24 2007-11-01 Jsr Corp 磁性粒子およびその製造方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57135801A (en) * 1980-12-23 1982-08-21 Boehringer Mannheim Gmbh Hydrophilic latex particles, manufacture and diagnostic drug containing same
JP2003322654A (ja) * 2002-02-27 2003-11-14 Hitachi Software Eng Co Ltd 生体高分子の検出方法
JP2005148048A (ja) * 2003-04-25 2005-06-09 Jsr Corp バイオチップおよびバイオチップキットならびにその製造方法および使用方法
JP2007211076A (ja) * 2006-02-08 2007-08-23 Jsr Corp 有機ポリマー粒子およびその製造方法、ならびにプローブ結合粒子
JP2007288133A (ja) * 2006-03-24 2007-11-01 Jsr Corp 磁性粒子およびその製造方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019511707A (ja) * 2016-02-18 2019-04-25 オプトフルイディクス インコーポレイテッド 流体試料中の粒子を特徴付けるためのシステム及び方法
JP7129909B2 (ja) 2016-02-18 2022-09-02 オプトフルイディクス インコーポレイテッド 流体試料中の粒子を特徴付けるためのシステム及び方法

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