WO2008111506A1 - 遺伝子導入細胞調製方法 - Google Patents

遺伝子導入細胞調製方法 Download PDF

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Publication number
WO2008111506A1
WO2008111506A1 PCT/JP2008/054141 JP2008054141W WO2008111506A1 WO 2008111506 A1 WO2008111506 A1 WO 2008111506A1 JP 2008054141 W JP2008054141 W JP 2008054141W WO 2008111506 A1 WO2008111506 A1 WO 2008111506A1
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WIPO (PCT)
Prior art keywords
cells
mass
transgenic cells
desired gene
preparing transgenic
Prior art date
Application number
PCT/JP2008/054141
Other languages
English (en)
French (fr)
Inventor
Ikuei Nukaya
Seung Shin Yu
Mikiko Sasaki
Emi Chatani
Tatsuji Enoki
Junichi Mineno
Ikunoshin Kato
Original Assignee
Takara Bio Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takara Bio Inc. filed Critical Takara Bio Inc.
Publication of WO2008111506A1 publication Critical patent/WO2008111506A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/461—Cellular immunotherapy characterised by the cell type used
    • A61K39/4611—T-cells, e.g. tumor infiltrating lymphocytes [TIL], lymphokine-activated killer cells [LAK] or regulatory T cells [Treg]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/463—Cellular immunotherapy characterised by recombinant expression
    • A61K39/4632—T-cell receptors [TCR]; antibody T-cell receptor constructs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
    • A61K39/4643—Vertebrate antigens
    • A61K39/4644—Cancer antigens
    • A61K39/46448—Cancer antigens from embryonic or fetal origin
    • A61K39/464482—Carcinoembryonic antigen [CEA]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
    • A61K39/4643—Vertebrate antigens
    • A61K39/4644—Cancer antigens
    • A61K39/464484—Cancer testis antigens, e.g. SSX, BAGE, GAGE or SAGE
    • A61K39/464486—MAGE
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C12N2501/515—CD3, T-cell receptor complex
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C12N2501/58—Adhesion molecules, e.g. ICAM, VCAM, CD18 (ligand), CD11 (ligand), CD49 (ligand)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Oncology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Biochemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

　生体への投与に有効な所望の遺伝子が導入された細胞集団の製造方法、当該製造方法により得られる細胞集団を提供する。詳細には、下記工程を包含することを特徴とする、所望の遺伝子が導入されたＴ細胞を含有する細胞集団の製造方法を提供する；（１）Ｔ細胞を含有する細胞集団をフィブロネクチンまたはそのフラグメントとＣＤ３リガンドの存在下で培養する工程、（２）所望の遺伝子を工程（１）の細胞集団に導入する工程。
PCT/JP2008/054141 2007-03-09 2008-03-07 遺伝子導入細胞調製方法 WO2008111506A1 (ja)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2007061047 2007-03-09
JP2007-061047 2007-03-09
JP2007223987 2007-08-30
JP2007-223987 2007-08-30

Publications (1)

Publication Number Publication Date
WO2008111506A1 true WO2008111506A1 (ja) 2008-09-18

Family

ID=39759445

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/054141 WO2008111506A1 (ja) 2007-03-09 2008-03-07 遺伝子導入細胞調製方法

Country Status (1)

Country Link
WO (1) WO2008111506A1 (ja)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009119793A1 (ja) * 2008-03-27 2009-10-01 タカラバイオ株式会社 遺伝子導入細胞の製造方法
WO2010087335A1 (ja) * 2009-01-27 2010-08-05 学校法人産業医科大学 Ｔ細胞抗原受容体遺伝子、受容体α鎖及びβ鎖発現用ベクター並びに細胞障害性Ｔ細胞
WO2011024791A1 (ja) 2009-08-25 2011-03-03 タカラバイオ株式会社 レチノイン酸存在下でのｔ細胞集団の製造方法
JP2018042481A (ja) * 2016-09-13 2018-03-22 テラ株式会社 抗原特異的ｔ細胞含有組成物及びその製造方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005061697A1 (ja) * 2003-12-22 2005-07-07 Hokkaido Technology Licensing Office Co., Ltd. 改変標的化ｔ細胞の製造方法及び医薬

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005061697A1 (ja) * 2003-12-22 2005-07-07 Hokkaido Technology Licensing Office Co., Ltd. 改変標的化ｔ細胞の製造方法及び医薬

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
"Takara Biotechnology Catalog 2002-2003", TAKARA BIOTECHNOLOGY CATALOG, 2002-2003, 2002 *
HIRAYAMA N. ET AL.: "Shuyo/Seiso Kogen MAGE-A4 Tokuiteki CTL Clone Yurai TCR Idenshi o Donyu shita T Saibo no Saibo Tokusei to Tokuisei", DAI 11 KAI SOCIETY FOR FUNDAMENTAL CANCER IMMUNOLOGY SOKAI SHOROKUSHU, 25 May 2007 (2007-05-25), pages 85, XP008145800 *
MURAKI N. ET AL.: "RetroNectin o Kumiawaseta T Saibo Kakudai Baiyo (I): Kakudai Baiyo ga Anteika shi, Naive T-yo Saibo ga Kohiritsu de Zoshoku (T-cell expansion using RetroNectin (I): Useful method to expand T-cells, characterization by high portion of Naive T-cell cells)", PROCEEDINGS OF THE JAPANESE CANCER ASSOCIATION, vol. 65, 28 August 2006 (2006-08-28), pages 330, XP003011558 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009119793A1 (ja) * 2008-03-27 2009-10-01 タカラバイオ株式会社 遺伝子導入細胞の製造方法
WO2010087335A1 (ja) * 2009-01-27 2010-08-05 学校法人産業医科大学 Ｔ細胞抗原受容体遺伝子、受容体α鎖及びβ鎖発現用ベクター並びに細胞障害性Ｔ細胞
WO2011024791A1 (ja) 2009-08-25 2011-03-03 タカラバイオ株式会社 レチノイン酸存在下でのｔ細胞集団の製造方法
JP2018042481A (ja) * 2016-09-13 2018-03-22 テラ株式会社 抗原特異的ｔ細胞含有組成物及びその製造方法

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