WO2008105454A1 - ラクリチン高発現細胞 - Google Patents

ラクリチン高発現細胞 Download PDF

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Publication number
WO2008105454A1
WO2008105454A1 PCT/JP2008/053396 JP2008053396W WO2008105454A1 WO 2008105454 A1 WO2008105454 A1 WO 2008105454A1 JP 2008053396 W JP2008053396 W JP 2008053396W WO 2008105454 A1 WO2008105454 A1 WO 2008105454A1
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Prior art keywords
lacritin
expressing
expressing cell
high level
vector
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PCT/JP2008/053396
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English (en)
French (fr)
Inventor
Takeshi Nakajima
Mitsuyoshi Azuma
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Senju Pharmaceutical Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Senju Pharmaceutical Co., Ltd. filed Critical Senju Pharmaceutical Co., Ltd.
Priority to JP2009501270A priority Critical patent/JPWO2008105454A1/ja
Priority to EP08720944A priority patent/EP2130915A4/en
Priority to US12/449,833 priority patent/US20100183572A1/en
Publication of WO2008105454A1 publication Critical patent/WO2008105454A1/ja

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/22—Hormones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Ophthalmology & Optometry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Physics & Mathematics (AREA)
  • Neurosurgery (AREA)
  • Plant Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Neurology (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

　本発明は、ラクリチンの、安定かつ大量供給可能な手段に関し、ラクリチンをコードするポリヌクレオチドを含有するベクターであって、当該ヌクレオチドがサイトメガロウイルス即時型初期エンハンサー／プロモーターおよびＳＶ４０後期ポリＡシグナルに作動可能に連結されているベクター、前記ベクターを細胞に導入してなるラクリチン発現細胞、前記ラクリチン発現細胞を培地中で培養する工程、および培養上清中のラクリチンを単離する工程を含む組換えラクリチンの製造方法、ならびに前記ラクリチン発現細胞を含有する眼疾患治療剤を提供する。
PCT/JP2008/053396 2007-02-28 2008-02-27 ラクリチン高発現細胞 WO2008105454A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2009501270A JPWO2008105454A1 (ja) 2007-02-28 2008-02-27 ラクリチン高発現細胞
EP08720944A EP2130915A4 (en) 2007-02-28 2008-02-27 CELL THAT CAN EXPRESS A LARGE QUANTITY OF LACRITIN
US12/449,833 US20100183572A1 (en) 2007-02-28 2008-02-27 Cell capable of expressing lacritin at high level

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007049936 2007-02-28
JP2007-049936 2007-02-28

Publications (1)

Publication Number Publication Date
WO2008105454A1 true WO2008105454A1 (ja) 2008-09-04

Family

ID=39721279

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/053396 WO2008105454A1 (ja) 2007-02-28 2008-02-27 ラクリチン高発現細胞

Country Status (4)

Country Link
US (1) US20100183572A1 (ja)
EP (1) EP2130915A4 (ja)
JP (1) JPWO2008105454A1 (ja)
WO (1) WO2008105454A1 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011007337A2 (en) 2009-07-16 2011-01-20 L'oreal Cosmetic use of lacritin-type polypeptides
WO2011037196A1 (ja) * 2009-09-25 2011-03-31 千寿製薬株式会社 ラクリチン遺伝子の転写調節因子のスクリーニング方法

Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996013974A1 (en) 1994-11-08 1996-05-17 Organogenesis Inc. In vitro tissue and organ equivalent models
WO2000029553A1 (en) 1998-11-19 2000-05-25 Organogenesis Inc. Bioengineered tissue constructs and methods for producing and using them
JP2001161353A (ja) 1999-12-09 2001-06-19 Japan Ophthalmic Consultants:Kk 移植用細胞片及びその作成方法
US20020039788A1 (en) 2000-02-29 2002-04-04 Isseroff Roslyn R. Corneal epithelial graft composites
WO2002065943A2 (en) 2001-02-20 2002-08-29 University Of Virginia Patent Foundation Ocular tear growth factor-like protein
WO2003009783A1 (fr) 2001-07-25 2003-02-06 Japan Tissue Engineering Co., Ltd. Lamines pour traitement de la cornee et leur procede de production
JP2003038170A (ja) 2001-07-26 2003-02-12 Mitsuo Okano 前眼部関連細胞シート、３次元構造体、及びそれらの製造法
WO2003026712A1 (fr) 2001-09-25 2003-04-03 Japan Science And Technology Agency Procede de formation de membrane basale, procede d'elaboration d'echantillon de membrane basale, tissu artificiel reconstitue utilisant cet echantillon, et procede d'elaboration de ce tissu
WO2003084431A2 (en) 2002-03-29 2003-10-16 Singapore Eye Research Institute Method for growth of human conjunctival tissue equivalents for research, clinical ocular surface transplantation and tissue engineering
WO2005119899A2 (en) * 2004-05-13 2005-12-15 University Of Virginia Patent Foundation Use of lacritin in promoting ocular cell survival
JP2006129724A (ja) * 2004-11-02 2006-05-25 Senju Pharmaceut Co Ltd ラクリチン活性を有する化合物のスクリーニング方法
WO2006129867A2 (en) * 2005-06-01 2006-12-07 Senju Pharmaceutical Co., Ltd. ENHANCED EXPRESSION OF LACTOFERRIN mRNA BY LACRITIN
JP2007049936A (ja) 2005-08-18 2007-03-01 Wakayama Univ 変異検出方法、変異検出プログラム及び記憶媒体

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5585265A (en) * 1992-11-30 1996-12-17 Gillette Company Human corneal epithelial cell lines with extended lifespan
US5827702A (en) * 1994-10-31 1998-10-27 Genentech, Inc. Ocular gene therapy
US20030087850A1 (en) * 2001-07-10 2003-05-08 Philip Dehazya Gene therapy for dry eye syndrome
US20050013806A1 (en) * 2003-04-04 2005-01-20 Chang Min S. Use of contact lens for corneal cell transplant
US8101169B2 (en) * 2005-02-23 2012-01-24 The Board Of Trustees Of The Leland Stanford Junior University Ocular gene therapy using avalanche-mediated transfection

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996013974A1 (en) 1994-11-08 1996-05-17 Organogenesis Inc. In vitro tissue and organ equivalent models
WO2000029553A1 (en) 1998-11-19 2000-05-25 Organogenesis Inc. Bioengineered tissue constructs and methods for producing and using them
JP2001161353A (ja) 1999-12-09 2001-06-19 Japan Ophthalmic Consultants:Kk 移植用細胞片及びその作成方法
US20020039788A1 (en) 2000-02-29 2002-04-04 Isseroff Roslyn R. Corneal epithelial graft composites
WO2002065943A2 (en) 2001-02-20 2002-08-29 University Of Virginia Patent Foundation Ocular tear growth factor-like protein
WO2003009783A1 (fr) 2001-07-25 2003-02-06 Japan Tissue Engineering Co., Ltd. Lamines pour traitement de la cornee et leur procede de production
JP2003038170A (ja) 2001-07-26 2003-02-12 Mitsuo Okano 前眼部関連細胞シート、３次元構造体、及びそれらの製造法
WO2003026712A1 (fr) 2001-09-25 2003-04-03 Japan Science And Technology Agency Procede de formation de membrane basale, procede d'elaboration d'echantillon de membrane basale, tissu artificiel reconstitue utilisant cet echantillon, et procede d'elaboration de ce tissu
WO2003084431A2 (en) 2002-03-29 2003-10-16 Singapore Eye Research Institute Method for growth of human conjunctival tissue equivalents for research, clinical ocular surface transplantation and tissue engineering
WO2005119899A2 (en) * 2004-05-13 2005-12-15 University Of Virginia Patent Foundation Use of lacritin in promoting ocular cell survival
JP2006129724A (ja) * 2004-11-02 2006-05-25 Senju Pharmaceut Co Ltd ラクリチン活性を有する化合物のスクリーニング方法
WO2006129867A2 (en) * 2005-06-01 2006-12-07 Senju Pharmaceutical Co., Ltd. ENHANCED EXPRESSION OF LACTOFERRIN mRNA BY LACRITIN
JP2007049936A (ja) 2005-08-18 2007-03-01 Wakayama Univ 変異検出方法、変異検出プログラム及び記憶媒体

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BRONDYK B. ET AL.: "pCI and pSI mammalian expression vectors", PROMEGA NOTES MAGAZINE, no. 49, 1994, pages 7 - 12, XP008117162 *
INVEST OPHTHALMOL VIS SCI., vol. 36, 1995, pages 614 - 621
SANGHI S. ET AL.: "cDNA and genomic cloning of lacritin, a novel secretion enhancing factor from the human lacrimal gland", J. MOL. BIOL., vol. 310, no. 1, 2001, pages 127 - 139, XP004464186 *
SANGHI, S. ET AL., JOURNAL OF MOLECULAR BIOLOGY, vol. 310, 2001, pages 127 - 139
See also references of EP2130915A4

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011007337A2 (en) 2009-07-16 2011-01-20 L'oreal Cosmetic use of lacritin-type polypeptides
WO2011007337A3 (en) * 2009-07-16 2012-08-16 L'oreal Cosmetic use of lacritin-type polypeptides
US9168215B2 (en) 2009-07-16 2015-10-27 L'oreal Cosmetic use of lacritin-type polypeptides
WO2011037196A1 (ja) * 2009-09-25 2011-03-31 千寿製薬株式会社 ラクリチン遺伝子の転写調節因子のスクリーニング方法

Also Published As

Publication number Publication date
EP2130915A1 (en) 2009-12-09
EP2130915A4 (en) 2010-04-21
JPWO2008105454A1 (ja) 2010-06-03
US20100183572A1 (en) 2010-07-22

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