WO2008101125A1 - Doublure de cuve de bioréacteur - Google Patents

Doublure de cuve de bioréacteur Download PDF

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Publication number
WO2008101125A1
WO2008101125A1 PCT/US2008/054023 US2008054023W WO2008101125A1 WO 2008101125 A1 WO2008101125 A1 WO 2008101125A1 US 2008054023 W US2008054023 W US 2008054023W WO 2008101125 A1 WO2008101125 A1 WO 2008101125A1
Authority
WO
WIPO (PCT)
Prior art keywords
liner
wall
volumetric capacity
bioreactor
vessel
Prior art date
Application number
PCT/US2008/054023
Other languages
English (en)
Inventor
Scott T. Broadley
Patricia R. Benton
Original Assignee
Broadley-James Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Broadley-James Corporation filed Critical Broadley-James Corporation
Publication of WO2008101125A1 publication Critical patent/WO2008101125A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements

Definitions

  • This application relates to bioreactor systems. More particularly, this application relates to a liner allowing placement and use of a bioreactor container of a certain capacity in a holding vessel having a larger capacity.
  • Bioreactors also referred to as fermenters
  • containers used for fermentation, enzymatic reactions, cell culture, tissue engineering, and food production, as well as in the manufacture of biologicals, chemicals, biopharmaceuticals, microorganisms, plant metabolites, and the like.
  • Bioreactors vary in size from benchtop models to large stand-alone units of holding thousands of liters of media.
  • the stringent asepsis requirements for sterile production in some bioreactors can require elaborate systems to achieve the desired product volumes. Consequently, the production of products in aseptic bioreactors can be costly which provides the motivation for pursuing improved systems.
  • reactors can be made of sheets of flexible material configured to form a disposable bioreactor bag.
  • the bag can be filled with media the desired amount of media, and may then "inflated" with a gas (e.g., air) that occupies the headspace and/or continually passes through the bag's headspace.
  • a gas e.g., air
  • Disposable bioreactors may include mechanical mechanisms for agitating the media.
  • sensors e.g., pH, temperature, and dissolved oxygen
  • devices e.g., for supplying liquid or gas into the media and/or removing a sample of media
  • Use of these mechanisms, sensors and devices may require that the bioreactor bag be supported within a cavity of a properly-sized rigid housing, such as a glass or metal bioreactor vessel.
  • Bioreactor bags of various volumetric capacities are used for different fermentation applications, and each sized bag requires a rigid vessel having a predetermined cavity size for holding a particular-sized bioreactor bag having a volumetric capacity that corresponds to the size of the cavity.
  • rigid bioreactor vessels can be expensive and require laboratory space for storage while not in use. Accordingly, there is a need for adapting a rigid bioreactor vessel having a defined and predetermined cavity size for use with a variety of differently- sized bioreactor bags that have smaller volumetric capacities than the cavity.
  • a liner for adjusting a vessel cavity size of a selected rigid bioreactor vessel, the selected vessel having a vessel cavity configured to operably hold a bioreactor bag having a first volumetric capacity.
  • the liner includes an inner wall defining a liner cavity, the inner wall at least partially surrounding the liner cavity and providing a contact surface that provides at least lateral support for a bioreactor bag placed in the liner cavity, such that the liner cavity can operably hold a bioreactor bag having a second volumetric capacity that is smaller than the first volumetric capacity.
  • the liner also includes an outer wall that at least partially surrounds the inner wall, the inner wall and outer wall being disposed relative to each other to define a selected liner thickness, the outer wall being configured such that the outer wall corresponds with the size of the vessel cavity so that the liner fits into the vessel cavity.
  • the second volumetric capacity is less than about half of the first volumetric capacity.
  • the inner wall is substantially circular in shape.
  • the liner comprises plastic.
  • the liner comprises polystyrene foam.
  • the liner comprises two or more sections disposed relative to each other to form the inner wall and the outer wall.
  • the inner wall and the outer wall are contiguous.
  • the liner is substantially cylindrical in shape.
  • the inner and outer wall extend to underlie the bottom of the cavity.
  • the liner comprises at least one flexible bag.
  • the liner comprises an enclosed volume between the inner wall and outer wall that is configured to hold a fluid.
  • the fluid can be a gas or a liquid.
  • the liner thickness can be variable and based on the amount of fluid in the enclosed volume.
  • the liner further includes one or more baffles disposed on the inner wall.
  • the one or more baffles can be disposed substantially parallel to a longitudinal axis of the liner.
  • the liner further comprises one or more temperature control elements disposed on the inner wall.
  • the one or more temperature control elements can be disposed substantially parallel to a longitudinal axis of the liner.
  • the one or more temperature control elements can comprise a resistive heating element, and/or a fluidic cooling or heating element.
  • a bioreactor system in a second aspect of the invention, includes a flexible plastic bioreactor bag, a holding vessel, and a liner.
  • the flexible plastic bioreactor bag has a first volumetric capacity.
  • the holding vessel is configured to receive the bioreactor bag, the holding vessel having a second volumetric capacity, the first volumetric capacity being less than the second volumetric capacity.
  • the liner is disposed inside the holding vessel, the liner configured to receive and substantially surround the bioreactor bag, the liner further configured to allow placement and use of the bag in the holding vessel.
  • the operative first volumetric capacity of the bioreactor bag is less than about 50% of the defined second volumetric capacity of the bioreactor vessel.
  • a liner for a bioreactor vessel having a first volumetric capacity to hold a bioreactor bag having a second volumetric capacity smaller than the first volumetric capacity.
  • the liner comprises an outer wall and an inner wall configured in a substantially cylindrical structure, the outer wall being sized to cooperate with an inner wall of a bioreactor vessel that receives and holds the liner therein, the inner wall being configured to substantially surround a cavity of the second volumetric capacity and provide lateral support of a bioreactor bag placed in the cavity.
  • the inner wall and the outer wall define a predetermined thickness, the thickness being selected to allow operative placement of the smaller volumetric capacity bioreactor bag in the larger volumetric capacity bioreactor vessel such that the smaller volumetric capacity bioreactor bag is held and laterally supported by the liner.
  • a method of operating a bioreactor comprises providing a flexible bioreactor bag having an operative first defined volumetric capacity, selecting a holding vessel having a defined second volumetric capacity larger than the first volumetric capacity, and disposing a liner inside the holding vessel, the liner having outer dimensions corresponding to the defined second volumetric capacity of the holding vessel and inner dimensions defining a cavity corresponding to the operative first defined volumetric capacity of the bioreactor bag.
  • the method also includes introducing the bioreactor bag inside the liner cavity, whereby the operative first defined volumetric capacity is realized by the cooperation of the bioreactor bag with the inner dimensions of the liner.
  • Figure 1 is a side elevation view showing a liner embodiment in a bioreactor vessel that supports a flexible bioreactor bag placed inside the liner.
  • Figure 2 is a cross-sectional view of the liner of Figure 1 taken along line 2-2 of Figure 1.
  • Figure 3 is a cross-sectional view of another embodiment of a liner.
  • Figure 4 is a cross-sectional view of another embodiment of a liner.
  • Figure 5 is a side elevation view illustrating a liner disposed in a rigid bioreactor vessel.
  • Figure 6 is a cross-sectional view of yet another embodiment of a liner.
  • Figure 7 is a perspective view of a portion of the liner illustrated in Figure 6, along line 7-7 of Figure 6.
  • Figure 8 is a partial perspective view of another embodiment of a portion of a liner illustrating one embodiment of baffles disposed on the inner wall of the liner.
  • a rigid vessel is typically used to operably hold the bioreactor bag in a certain position (e.g., upright) in a cavity of the rigid vessel.
  • the cavity is a predetermined size to operably hold and provide lateral support for a selected bioreactor bag of a particular size.
  • the selected bioreactor bag when placed in the cavity and filled with media, achieves dimensions that correspond to the dimensions of the rigid vessel cavity.
  • a liner placed within the cavity can adapt the size of the cavity to can hold bags of various smaller sizes (e.g., bioreactor bags having volumetric media capacities equal to or smaller than the defined volumetric capacity of the rigid vessel), based on
  • the liner has an inner wall and an outer wall, the inner wall defining a cavity of the liner in which to place and hold a bioreactor bag.
  • the outer wall can surround the inner wall, and in use corresponds with the cavity of the rigid vessel such that at least a portion of the outer wall contacts a wall of the vessel cavity.
  • a particular liner can be selected for use based on the size of the cavity defined by the inner wall and the size of the cavity into which the liner will fit (e.g., defined by the size of the outer wall).
  • the particular dimensions of the liner inner and outer wall, and the liner "thickness" can be selected to enable a desired "adjustment" of a rigid vessel cavity so that the rigid vessel can operably hold smaller bioreactor bags.
  • Some liner embodiments are made from plastic or foam, or another material that is typically easy to clean and relatively inexpensive.
  • a liner can be formed as a single piece, or it can be made from two or more sections that are positioned to form an inner wall defining a cavity and an outer wall.
  • a liner may be also be made from flexible material which is formed to enclose a volume to hold a fluid; in this embodiment, the size and/or thickness of the liner may be adjusted based on the amount of fluid placed inside the liner.
  • liners provide for systems and methods of adjusting the capacity of a rigid bioreactor vessel to hold and/or support media containers of various size and shapes. These embodiments can have a variety of applications to cell fermentation processes, and also in such areas as food production, chemical production, biopharmaceutical production, and the like. Such embodiments may be used with bioreactor systems ranging in size and capacity from small, benchtop-sized bioreactors of about 100 milliliters up to about 5000 liter production-scale bioreactors.
  • Figure 1 illustrates an example of a bioreactor system which includes a container 102 for holding media placed in the cavity of a liner 10.
  • the liner 10 is operably disposed in a rigid vessel 104 that holds and supports the liner 10.
  • the container 102 can be, for example, a flexible or semi-flexible bioreactor bag, or another sterilizable container that is configured for holding media and that benefits from at least some support to maintain a certain position (e.g., upright) during fermentation.
  • the media holding container 102 is a flexible bioreactor bag.
  • the liner 10 may have a configuration that is selected to at least partially surround and laterally support the flexible bag 102 while the liner 10 is disposed in the cavity 110 of the rigid vessel 104.
  • the liner 10 includes an inner wall 108 that partially surrounds and defines a cavity 110.
  • the dimensions of the cavity 110 e.g., diameter and/or height
  • the inner wall 108 at least partially contacts and supports the bioreactor bag 102, holding the bag 102 "upright” and providing lateral support for the sides of the bioreactor bag 102.
  • the liner 10 also includes an outer wall 106 that at least partially surrounds the inner wall 108.
  • the liner is cylindrical-shaped, but other liner shapes are also contemplated. Some embodiments of other liner shapes are described below in reference to Figures 3-8.
  • the outer wall 108 is sized so that the liner 10 fits inside of and corresponds to the rigid vessel 104, so that at least a portion of the outer wall 108 contacts the rigid vessel enabling the rigid vessel to hold and support the liner 10.
  • a diameter dimension of the outer wall can correspond to a diameter dimension of the cavity in the rigid vessel 104.
  • the height of the liner 10 is selected so that the entire liner fits within the cavity 110 of rigid vessel 104.
  • a shape of the liner 10 may be shaped or include elements that affect the shape of a flexible bioreactor bag supported within the cavity 110 and may change an agitation characteristic of the bioreactor bag 102.
  • Figure 2 illustrates a cross-sectional view of the liner 10 shown Figure 1.
  • the liner 10 comprises the outer wall 106 and the inner wall 108, each having a generally circular cross section.
  • the liner 10 may further comprise one or more holes or channels (not shown) configured to allow passage of tubes and/or ports through the liner 10 to connect to the bag 102.
  • the use of the liner 10 allows the rigid vessel 104 to accommodate and support a flexible bag 102 smaller in dimension (e.g., diameter) than the inside dimensions (e.g., diameter) of the rigid vessel 104.
  • Embodiments of a liner may comprise any material suitable for supporting a filled or partially filled bioreactor bag inside a vessel, including plastic, polystyrene foam, plastic-coated polystyrene foam, or any other suitable foam.
  • the liner 10 may also comprise metal.
  • the liner 10 may be solid or completely or partially hollow.
  • the liner 10 may comprise flexible or semi-flexible material formed into one or more bags having an enclosed volume. The volume is capable of being filled with and hold a gas or liquid, for example, a coolant.
  • filling the liner volume with a selected amount of gas or liquid can adjust the thickness of the liner to a particular desired size, and adapt a rigid vessel cavity of one size to hold a variety of different size bioreactor bags.
  • a suitably sized liner may enable a rigid vessel dimensioned to hold twenty liter bioreactor bags to hold fifteen liter or ten liter bags, depending on the amount of fluid placed in the enclosed liner volume.
  • fluid may be circulated through the liner to provide heating and/or cooling of media inside a bioreactor bag placed within the liner.
  • Figure 3 illustrates a cross-section of another embodiment of a liner 30 having a different shaped volume than the liner 10 illustrated in Figures 1 and 2.
  • liner 30 has a circular-shaped outer wall 306 and a lenticular-shaped inner wall 308.
  • the liner 30 is configured with a much larger "thickness" than the liner 10 illustrated in Figure 2, which can allow a rigid vessel to operably hold a bioreactor bag that is much smaller than it is dimensioned to hold without a liner.
  • the liner inner wall may be formed into other possible cross-sectional shapes, including elliptical, curvilinear and polygonal.
  • Figure 3 also illustrates that the liner thickness may vary within different portions of the liner.
  • any of the liner configurations can comprise one section, or two or more sections that when used together provide the desired form of the liner.
  • Figure 4 illustrates one embodiment of a circular-shaped liner 40 that comprises four sections 410a- d. In some applications, the sections can be placed together to form the liner 40, as shown in Figure 4.
  • the liner 40 includes an inner wall 408 and an outer wall 406 that at least partially surrounds the inner wall 408.
  • the inner wall 408 defines and at least partially surrounds a cavity 411 in which a bioreactor bag is operably held, the four sections 410a-d providing lateral support for a bioreactor bag placed within the cavity 411.
  • Each section may be connected to an adjoining section, or may unconnected and held in place by the rigid vessel and the bioreactor bag placed within the cavity formed by the four sections 410a-d.
  • the liner 40 formed of separate sections can advantageously reduce the storage space required for the liner.
  • the sections of the liner 40 can be made to stack together to further facilitate storage.
  • some embodiments include a liner 50 comprising one or more wall sections 512, as well as a floor section 514 configured to support the bottom of a flexible bioreactor bag placed in cavity 511 (not shown in the figure) when the bag is filled or partially filled with media.
  • the wall sections 512 and the floor section 514 surround the cavity 511 except for a top opening 513.
  • the floor 514 may be integrally formed with the wall section 512, or may be separate from the rest of the liner 50.
  • the floor section 514 may have the same thickness as the wall section 512. In other embodiments of the invention, the floor section 514 may have a lesser or greater thickness than the wall section 512.
  • a liner 60 may comprise two or more sections 610, each having an inner wall 608.
  • liner 60 comprises four sections.
  • Figure 6 illustrates liner 60 with sections 610 formed into the generally circular liner 60.
  • Figure 7 illustrates one of the four sections of liner 60 in a perspective view.
  • One or more of the sections 610 may have one or more protrusions or baffles 620 located on its inner wall 608.
  • the baffles 620 may be configured to create deformation (e.g., dimples) in the walls of a bioreactor bag placed inside the liner 60, advantageously creating a contoured surface on the wall of the bioreactor bag that may create turbulence in media held therein, which can help prevent "funnels" from forming in the media when the media is agitated (e.g., by a rotating agitator).
  • the baffles 620 may be disposed vertically along the length of the section 610, as shown in the Figure 6.
  • Other embodiments of baffles 620 may be configured in a variety of shapes that can also cause a deformation of the bioreactor bag, including, but not limited to, shapes that have a cross- sectional curvilinear shape.
  • the baffles 620 are integrally formed with the sections 610.
  • the baffles may be removably or permanently attached to one or more liner sections 610.
  • one or more of the sections 610 of the liner 60 may comprise one or more heating elements 630 disposed along an inner wall 608.
  • the heating elements 630 are configured to be in thermal communication with media inside bioreactor bag placed within liner 60, such that the heating elements 630 affect the temperature to adjust or maintain the temperature of the media inside the bioreactor bag
  • the heating elements 630 may comprise, for example, silicon rubber strips having wires running through them to provide resistive-style heating.
  • the heating elements 630 can also comprise lines (not shown) capable of carrying hot or cold fluid that through thermal conduction affect the temperature of media inside a bioreactor bag.
  • the heating elements 630 may be disposed vertically along the length of the section 610, or may be disposed in any other manner suitable for heating or cooling the contents of a bag placed inside the liner 60.
  • a liner 80 may comprise baffles 820 disposed circumferentially around a vertical central axis of the liner 80.
  • the baffles need not be continuous along the length or inner circumference of a liner, but instead may comprise one or more individual protuberances disposed on the inner wall of the liner.
  • embodiments of the invention may advantageously be used to allow placement and use of a flexible bioreactor bag of a given capacity in a rigid bioreactor vessel of another, larger capacity.
  • an embodiment of the present invention may be used to allow placement and use of a five liter capacity bioreactor bag in a fifteen liter capacity bioreactor vessel.
  • Embodiments of various sized liners described herein are merely examples of some uses of the liner.
  • Various embodiments of the described liners can be used to adjust the cavity of rigid vessels having cavity capacities of up to about 5000 liters (or more) and as small as rigid vessels for bench-top use.
  • liners Using various embodiments of the liners, a variety of different flexible bioreactor bags can be used with the same rigid vessel, avoiding the expense of purchasing properly-sized rigid vessels for each size of bag.
  • Embodiments of the liners having baffles also desirably reduce the formation of funnels during agitation of media in a bioreactor bag.
  • Embodiments of the invention also comprise methods of adjusting the capacity of a rigid bioreactor vessel.
  • a vessel liner is provided and placed in a rigid bioreactor vessel of a given capacity.
  • the vessel liner defines a vessel cavity which is configured to hold and/or support a filled or partially filled bioreactor bag of a smaller capacity than is the vessel itself.
  • the vessel liner can have an outer wall or surface that is configured to correspond to the shape of an interior of the rigid bioreactor vessel.
  • the outer wall or surface need not correspond exactly to the shape of the vessel interior; for example, the outer wall can comprise ridges or protrusions configured to contact the vessel interior.
  • the vessel liner can also have an inner wall or surface configured to provide lateral and/or subjacent support to the bioreactor bag.
  • the inner wall or surface need not correspond exactly to the shape of the bioreactor bag; for example, the inner wall can include one or more baffles, protrusions, or heating elements configured to affect the processing of the media in the bag.

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  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne une doublure (10) pour ajuster la capacité d'une cuve de bioréacteur rigide (104). La doublure (10) comporte une paroi interne (108) définissant une cavité de doublure et une paroi externe (106) qui entoure au moins en partie la paroi interne (108). La paroi interne (108) comprend une surface de contact qui assure le support latéral pour un sac de bioréacteur (102) placé dans la cavité de doublure. La paroi interne (108) et la paroi externe (106) sont disposées l'une par rapport à l'autre de manière à définir une épaisseur de doublure. La paroi externe (106) correspond à la dimension de la cavité de cuve de sorte que la doublure s'adapte dans la cuve (104). La cavité de doublure est apte à contenir et maintenir un sac de bioréacteur (102) d'une capacité inférieure à celle de la cuve.
PCT/US2008/054023 2007-02-15 2008-02-14 Doublure de cuve de bioréacteur WO2008101125A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US89016607P 2007-02-15 2007-02-15
US60/890,166 2007-02-15

Publications (1)

Publication Number Publication Date
WO2008101125A1 true WO2008101125A1 (fr) 2008-08-21

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PCT/US2008/054023 WO2008101125A1 (fr) 2007-02-15 2008-02-14 Doublure de cuve de bioréacteur

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017013974A1 (fr) * 2015-07-22 2017-01-26 株式会社日立製作所 Dispositif d'agitation
WO2020260092A1 (fr) * 2019-06-24 2020-12-30 Global Life Sciences Solutions Usa Llc Système de support de bioréacteur

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5362642A (en) * 1993-02-10 1994-11-08 Hyclone Laboratories Methods and containment system for storing, reconstituting, dispensing and harvesting cell culture media
US6190913B1 (en) * 1997-08-12 2001-02-20 Vijay Singh Method for culturing cells using wave-induced agitation
US20010031491A1 (en) * 1998-09-01 2001-10-18 Curtis Wayne R. Method and apparatus for aseptic growth of cells and/or microorganisms
US20060270036A1 (en) * 2005-04-22 2006-11-30 Hyclone Laboratories, Inc. Gas spargers and related container systems

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5362642A (en) * 1993-02-10 1994-11-08 Hyclone Laboratories Methods and containment system for storing, reconstituting, dispensing and harvesting cell culture media
US6190913B1 (en) * 1997-08-12 2001-02-20 Vijay Singh Method for culturing cells using wave-induced agitation
US20010031491A1 (en) * 1998-09-01 2001-10-18 Curtis Wayne R. Method and apparatus for aseptic growth of cells and/or microorganisms
US20060270036A1 (en) * 2005-04-22 2006-11-30 Hyclone Laboratories, Inc. Gas spargers and related container systems

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017013974A1 (fr) * 2015-07-22 2017-01-26 株式会社日立製作所 Dispositif d'agitation
WO2020260092A1 (fr) * 2019-06-24 2020-12-30 Global Life Sciences Solutions Usa Llc Système de support de bioréacteur

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