WO2008091821A2 - Reducing acrylamide formation in thermally processed foods - Google Patents
Reducing acrylamide formation in thermally processed foods Download PDFInfo
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- WO2008091821A2 WO2008091821A2 PCT/US2008/051578 US2008051578W WO2008091821A2 WO 2008091821 A2 WO2008091821 A2 WO 2008091821A2 US 2008051578 W US2008051578 W US 2008051578W WO 2008091821 A2 WO2008091821 A2 WO 2008091821A2
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- asparagine
- potato
- extract
- deficient
- acrylamide
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/10—Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops
- A23L19/12—Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops of potatoes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/10—Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops
- A23L19/12—Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops of potatoes
- A23L19/18—Roasted or fried products, e.g. snacks or chips
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/10—General methods of cooking foods, e.g. by roasting or frying
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/21—Removal of unwanted matter, e.g. deodorisation or detoxification by heating without chemical treatment, e.g. steam treatment, cooking
Definitions
- the present invention relates to a method for reducing the amount of asparagine, a pre-cursor of acrylamide in a food product.
- This invention permits the production of foods having significantly reduced levels of acrylamide, and more particularly the invention is directed towards a method for leaching at least one acrylamide pre-cursor with an extract that is deficient in the acrylamide pre-cursor being leached.
- Description of Related Art [0004]
- the chemical acrylamide has long been used in its polymer form in industrial applications for water treatment, enhanced oil recovery, papermaking, flocculants, thickeners, ore processing and permanent-press fabrics.
- Acrylamide precipitates as a white crystalline solid, is odorless, and is highly soluble in water (2155 g/L at 30° C).
- Synonyms for acrylamide include 2-propenamide, ethylene carboxamide, acrylic acid amide, vinyl amide, and propenoic acid amide.
- Acrylamide has a molecular mass of 71.08, a melting point of 84.5°C, and a boiling point of 125°C at 25 mmHg.
- CFLAY.00344PCT -1- PCT Application unhealed and boiled foods.
- Reported levels of acrylamide found in various similarly processed foods include a range of 330 - 2,300 ( ⁇ g/kg) in potato chips, a range of 300 - 1 100 ( ⁇ g/kg) in French fries, a range 120 - 180 ( ⁇ g/kg) in com chips, and levels ranging from not detectable up to 1400 ( ⁇ g/kg) in various breakfast cereals.
- acrylamide is formed from the presence of amino acids and reducing sugars.
- Asparagine accounts for approximately 40% of the total free amino acids found in raw potatoes, approximately 18% of the total free amino acids found in high protein rye, and approximately 14% of the total free amino acids found in wheat.
- acrylamide in foods is a recently discovered phenomenon, its exact mechanism of formation has not been confirmed.
- the Maillard reaction has long been recognized in food chemistry as one of the most important chemical reactions in food processing and can affect flavor, color, and the nutritional value of the food.
- the Maillard reaction requires heat, moisture, reducing sugars, and amino acids.
- the Maillard reaction involves a series of complex reactions with numerous intermediates, but can be generally described as involving three steps.
- the first step of the Maillard reaction involves the combination of a free amino group (from free amino acids and/or proteins) with a reducing sugar (such as glucose) to form Amadori or Heyns rearrangement products.
- the second step involves degradation of the Amadori or Heyns rearrangement products via different alternative routes involving deoxyosones, fission, or Strecker degradation.
- a complex series of reactions including dehydration, elimination, cyclization, fission, and fragmentation result in a pool of flavor intermediates and flavor compounds.
- the third step of the Maillard reaction is characterized by the formation of brown nitrogenous polymers and co- polymers.
- Figure 1 illustrates a simplification of suspected pathways for the formation of acrylamide starting with asparagine and glucose.
- Acrylamide has not been determined to be detrimental to humans, but its presence in food products, especially at elevated levels, is undesirable. As noted previously, relatively higher concentrations of acrylamide are found in food products that have been heated or thermally processed. The reduction of acrylamide in such food products could be accomplished by reducing or eliminating the pre-cursor compounds that form acrylamide, inhibiting the formation of acrylamide during the processing of the food, breaking down or reacting the acrylamide monomer once formed in the food, or removing acrylamide from the product prior to consumption. Understandably, each food product presents unique challenges for accomplishing any of the above options. For example, foods that are sliced and cooked as coherent pieces may not be readily mixed with various additives without physically destroying the cell structures that give the food products their unique characteristics upon cooking. Other processing requirements
- FIG. 2 illustrates well known prior art methods for making fiied potato chips from raw potato stock.
- the raw potatoes which contain about 80% or more water by weight, first proceed to a peeling step 21. After the skins are peeled from the raw potatoes, the potatoes are then transported to a slicing step 22.
- the thickness of each potato slice at the slicing step 22 is dependent on the desired the thickness of the final product.
- An example in the prior art involves slicing the potatoes to a thickness of about 0.04 to about 0.08 inches. These slices are then transported to a washing step 23, wherein the surface starch on each slice is removed with water. The washed potato slices are then transpoited to a cooking step 24.
- This cooking step 24 typically involves frying the slices in a continuous fryer at, for example, about 173 0 C to about 182°C (340-360 0 F) for approximately two to three minutes.
- the cooking step generally reduces the moisture level of the chip to less than 2% by weight.
- a typical fried potato chip exits the fryer with approximately 1 -2% moisture by weight.
- the cooked potato chips are then transpoited to a seasoning step 25, where seasonings arc applied in a rotation drum.
- seasoning step 25 seasonings arc applied in a rotation drum.
- the seasoned chips proceed to a packaging step 26.
- This packaging step 26 usually involves feeding the seasoned chips to one or more weighers which then direct chips to one or more vertical form, fill, and seal machines for packaging in a flexible package.
- fabricated snack means a snack food that uses as its starting ingredient something other than the original and unaltered starchy starting material.
- fabricated snacks include fabricated potato chips that use a dehydrated potato product as a starting material and corn chips which use a masa flour as its starting material. It is noted here that the dehydrated potato product can be potato flour, potato flakes, potato granules, or any other form in which dehydrated potatoes exist. When any of these terms are used in this application, it is understood that all of these variations are included.
- a fabricated potato chip does not require the peeling step 21, the slicing step 22, or the washing step 23. Instead, fabricated potato chips start with a dehydrated potato product such as potato flakes. The dehydrated potato product is mixed with water and other minor ingredients to form a dough. This dough is then sheeted and cut before proceeding to a cooking step. The cooking step may involve frying or baking. The chips then proceed to a seasoning step and a packaging step.
- the mixing of the potato dough generally lends itself to the easy addition of other ingredients. Conversely, the addition of such ingredients to a raw food product, such as potato slices, requires that a mechanism be found to allow for the
- U.S. Patent No. 3,934,046 (“Weaver”), does not specifically address a method for reducing acrylamide formation but, its teachings are pertinent to the problem at hand. It is well known in the art that the browning of thermally processed foods is caused in part by the heating of amino acids, such as asparagine, in the presence of reducing sugars. As explained in Weaver, "the browning tendency of the tubers increases as the content of reducing sugars increases. It has also been demonstrated that the reducing sugars react with the nitrogenous constituents in the potatoes, producing dark-colored reaction products.” Weaver teaches that leaching raw potato pieces with hot water reduces the browning that occurs in the cooking phase.
- This present invention is a method for reducing the amount of asparagine in a potato products comprising in one embodiment: continuously providing a substantially untreated potato feed having a first concentration of asparagine and leaching asparagine out of said untreated potato feed with an asparagine-deficient potato extract, thereby forming a post-wash extract containing asparagine, as well as a treated potatoes having a second concentration of asparagine that is less than said first concentration.
- the method further comprises removing asparagine from said post- wash extract with at least one asparagine removal unit, thereby regenerating said asparagine-deficient potato extract and reusing said asparagine- deficient potato extract.
- the post-wash extract can be regenerated to reduce asparagine or other acrylamide pre-cursors by one or more methods such as using an enzyme, such as asparaginase, to breakdown the acrylamide pre-cursor asparagine into reaction products to reduce the concentration of the pre-cursor.
- an enzyme such as asparaginase
- the resultant reaction products can then be removed in later unit operations.
- Another method that can be employed to remove the acrylamide precursor is use of an ion exchange resin.
- the leaching liquid is ultrasonically vibrated while it is in contact with the potato pieces.
- acrylamide pre-cursors, along with other water-soluble compounds are non-selectively leached out of the raw potato pieces by pure water, in a first contacting stage. Next, the acrylamide pre-cursors are removed from the leaching extract, and the previously leached, desirable compounds are then returned to the potato pieces in a second contacting stage.
- the invention provides a method of reducing asparagine in a food ingredient comprising the steps of providing a food ingredient having a first concentration of asparagine, selectively leaching asparagine out of said food ingredient with an asparagine- deficient food extract, such that said food ingredient has a second concentration of asparagine that is less than said first concentration.
- Figure 1 is a schematic of suspected chemical pathways for acrylamide formation
- Figure 2 is a schematic of prior art potato chip processing steps
- Figure 3 is a graph showing, on the y-axis in parts per billion ("ppb"), acrylamide concentrations of potato test samples that were fried after contacting in various ways described along the x-axis, as well as the final moisture content by weight;
- Figure 4 is a graph comparing the original results from Figure 3 with the Figure 3 results after normalization to a moisture content of about 1.32% by weight;
- Figure 5 is a graph showing the relationship between acrylamide concentration and final fried product moisture wherein the acrylamide concentration in ppb is on the y-axis, and the moisture content in weight percent is on the x-axis;
- Figure 6 is a graph showing the relationship between acrylamide concentration and final baked product moisture wherein the acrylamide concentration in ppb is on the y-axis, and the moisture content in weight percent is on the x-axis;
- Figure 7 a is a graph showing the acrylamide concentrations in potato test samples that were par- fried and then oven-dried at about 120 0 C (250 0 F) after various methods of
- Figure Ib is a graph showing the last six data points of Figure Ia on a narrower acrylamide concentration scale
- Figure 8 is a graph showing the data from Figure Ia after normalizing the par- fry data to a moisture level of about 3.13% by weight and normalizing the oven-dry data to a moisture level of about 1.25% by weight;
- Figure 9 is a graph showing on the y-axis in ppb: 1) the acrylamide levels of potato test samples that were contacted in the various ways shown on the x-axis, then par- fried at about 178°C (353 0 F), and 2) the acrylamide levels of those same potato test samples after oven- drying at about 176°C (350 0 F), normalized to a moisture level of about 0.76% by weight;
- Figure 10 is a chart showing the operating conditions and results of an experiment in which a control sample of potato slices was atmospheric fried to about 1.4% moisture by weight, and a test sample was atmospheric fried to about 2.5% moisture by weight, then oven- dried to about 1.4% moisture by weight;
- Figure 11 is a chart showing the operating conditions and results of several experiments in which a control sample of potato slices was atmospheric fried to about 0.8% moisture by weight, and four test samples were atmospheric par-fried to about 3-10% moisture by weight, then low-temperature vacuum fried to below 1 % moisture by weight; and
- Figure 12 is a chart showing the operating conditions and results of seven experiments in which four test samples were atmospheric fried in oil with initial temperatures ranging from about 165 0 C to about 180 0 C (329°F-356°F) for about 3-4 minutes, and three test samples were low-temperature vacuum fried for about 4-10 minutes at temperatures ranging
- Figure 13a is a schematic diagram of a system and method for leaching asparagine out of a continuous feed of raw potato pieces according to one embodiment of the present invention
- Figure 13 b is a schematic diagram of a unit and method for regenerating a precursor-removing unit saturated with acryl amide pre-cursors according to another embodiment of the invention.
- Figure 14 is a schematic diagram of a system and method for non-selectively leaching water-soluble compounds from a continuous feed of raw potato pieces into a stream of water, removing asparagine from the stream of water, and returning some of the previously- leached water-soluble compounds to the potato pieces according to another embodiment of the invention.
- Figure 15 is a schematic diagram of a system and method adding an enzyme to the de-starched, post-wash extract to provide an acrylamide pre-cursor deficient solution, contacting raw food or untreated potato pieces having a native concentration of asparagine in the acrylamide pre-cursor deficient solution to preferentially leach out the acrylamide pre-cursor from the untreated food pieces such that the raw food piece has a reduced concentration of asparagine that is less than the native concentration, and removing the treated food pieces from the extraction unit.
- amino acids that are the building blocks of proteins and can be found in these food ingredients including, but not limited to, lysine, alanine, asparagine, glutamine, arginine, histidine, glycine and aspartic acid.
- thermally processed is meant food or food ingredients wherein components of the food, such as a mixture of food ingredients, are heated to food temperatures of at least 12O 0 C at ambient pressure. Thermally processing can also encompass lower temperatures at pressures below atmospheric. The food ingredient may be separately processed at elevated temperature prior to the formation of the final food product.
- the thermally- processed foods include, by way of example and without limitation, all of the foods previously listed as examples of fabricated snacks and fabricated foods, as well as French fries, yam fries, other tuber or root materials, cooked vegetables including cooked asparagus, onions, and tomatoes, coffee beans, cocoa beans, cooked meats, dehydrated fruits and vegetables, heat- processed animal feed, tobacco, tea, roasted or cooked nuts, soybeans, molasses, sauces such as barbecue sauce, plantain chips, apple chips, fried bananas, and other cooked fruits.
- thermally processed food ingredients include processed oats, par-boiled and dried rice, cooked soy products, corn masa, roasted coffee beans and roasted cacao beans.
- raw food ingredients can be used in the preparation of the final food product wherein the production of the final food product includes a thermal heating step.
- a thermal heating step is the manufacture of potato chips from raw potato slices by the step of frying at a temperature of from about 120 0 C to about 22O 0 C or the production of French fries or other fried food products at similar temperatures.
- a significant formation of acrylamide has been found to occur when the amino acid asparagine is heated in the presence of a simple sugar.
- a reduction of acrylamide in thermally processed foods can be achieved by inactivating the asparagine.
- inactivating is meant removing asparagine from the food or rendering asparagine non-reactive along the acrylamide formation route by means of conversion or binding to another chemical that interferes with the formation of acrylamide from asparagine.
- One such method for inactivating is to contact asparagine with the enzyme asparaginase.
- This enzyme decomposes asparagine to aspartic acid and ammonia.
- Asparagine may also be inactivated as the pre-cursor of acrylamide in a thermally processed food by leaching.
- the solubility of asparagine in an aqueous solution can be facilitated when the pH of the solution is maintained as slightly acidic or slightly basic, preferably between a pH of about 5
- CFLAY.00344PCT -14- PCT Application and about 6.5 and between about 7.5 and about 9.0 at ambient temperature.
- the solubility of asparagine can also be facilitated by supplying an elevated temperature of between about 100 0 F (37°C) and about 150 0 F.
- Asparagine may further be inactivated as the pre-cursor of acrylamide in a thermally processed food by fermentation.
- Asparagine can also be incorporated into proteins to inactivate asparagine as a pre-cursor to acrylamide.
- Asparagine may be further inactivated as the pre-cursor of acrylamide by the addition of a pH reducing salt such as calcium lactate, calcium chloride or calcium malate.
- CFLAY.00344PCT -15- PCT Application about 80 ° C oven for about 40 hours to evaporate to dryness. After heating, about 0.2 ml of water was added to each vial. The vials were then heated in a gas chromatographic oven with the following temperature profile: proceeding from an initial temperature of about 40°C; heating about 20°C per minute to about 200°C; and holding at about 200°C for about 2 minutes before cooling to about 40°C. The reaction mixtures were then extracted with about 50 ml water and acrylamide in the water was measure by GC-MS. The values measured are shown in Table 1 below:
- plant-derived food ingredients can also be sourced from plants that are bred and selected for having asparagine levels that are lower than those of other similar plants. A reduction in the amount of asparagine in the plant-derived food ingredient will be reflected in the amount of acrylamide that is formed under the same conditions of thermal treatment.
- each one of the potato chip processing steps (the peeling step 21, the slicing step 22, the washing step 23, the cooking step 24, the seasoning step 25, and the packaging step 26) is considered a separate unit operation with regard to the overall process of producing a potato chip food product.
- a first example of the manipulation of a unit operation involves the washing step 23 (illustrated in Figure 2) of potato chips produced by slicing raw potato stock.
- the prior art method of washing slices involves rinsing the chips with water at room temperature.
- the average residence time of each chip in this water rinse in the prior art is typically less than about 60 seconds, depending on the equipment used.
- Figure 3 illustrates how the chip washing unit operation can be manipulated such that acrylamide levels in the finished chip product can be adjusted.
- the washing step 23 can be manipulated to comprise a contacting step, where a
- FIG. 3 is a chart showing on the left (from the viewer's perspective) vertical or y-axis, the amount of acrylamide ("AA”) in parts per billion (“ppb") found in the finished potato chip product.
- the right vertical or y-axis of the graph in Figure 3 shows the percent moisture by weight in the finished chip product.
- the acrylamide level is charted on the graph by the vertical bars, while the percent moisture level is charted by the line plot.
- the horizontal or x- axis of the chart shown in Figure 3 lists various processing parameter changes made to the washing unit operations of a potato chip manufacturing process.
- the cooking time and temperature were identical for all product runs reflected in Figure 3. Specifically, each sample was fried at about 178 0 C (353°F) for about 120-140 seconds. Consequently, the moisture levels of the end product tended to vary.
- the next data point 32 shown on the graph in Figure 3 reflects a change in the washing step that comprises contacting the potato slices with water as the aqueous solution, increasing the contact time of the aqueous solution with the potato slices to ten minutes, and increasing the temperature of the aqueous solution from ambient or room temperature to about 38°C (100 0 F).
- This adjustment resulted in a decrease of the acryl amide in the finished product to approximately 210 ppb and a reduction in the moisture level of the finished product to less than 1 % by weight.
- the third data point 33 reflects that increasing the aqueous solution (again, water) temperature to about 54°C (13O 0 F) with an average contact time of five minutes did not result in appreciable reduction in aery] amide levels in the finished product.
- the fourth data point 34 demonstrates an appreciable reduction in acrylamide levels in the final product (below 100 ppb) when the washing unit operation involves a contacting step providing one minute contact time with an aqueous solution comprising water at a temperature of about 82°C (180 0 F).
- the moisture level of the end-product chip was nearly 1.8%.
- the fifth data point 35 reflects that using a 1% L-Cysteine solution as the aqueous solution, at ambient temperatures for fifteen minutes, reduces the acrylamide level in the final product to less than 250 ppb.
- the vertical or y- axis is again labeled in ppb of acrylamide found in the finished product.
- the horizontal or x- axis is labeled to show the parameters of each data point.
- each data point shows a pair of vertical bars, the bars on the left of a pair are imported from Figure 3 while the bars on the right of a pair reflect the expected results of the same contacting process parameters if the final product were fried to a uniform or standardized moisture level of 1.32%.
- the first data point 41 is the base sample involving a two to three minute water wash at ambient temperature.
- the second data point 42 involves the contacting step according to the present invention, where the potato slices are contacted with an aqueous solution comprising water at a temperature of about 38 0 C (100 0 F) for a ten minute contact time.
- the left-hand bar again reflects that such contacting followed by frying at approximately 178 0 C (353°F) for about 120- 130 seconds will result in just over 200 ppb acrylamide in the finished product and a finished product having a moisture level of less than 1%.
- the right-hand bar demonstrates that if a chip thus contacted were fried to a standardized moisture level of 1.32%, the projected aciy ⁇ amide level would drop to approximately 150 ppb.
- One embodiment of the present invention comprises a method for removing asparagine by using an asparagine- deficient potato extract or solution to leach asparagine out of raw potato pieces before cooking.
- the terms 'pre-cursor' and 'asparagine' can be used interchangeably in this description, since asparagine has been identified as the single pre-cursor most directly linked to acrylamide formation.
- the invention can likewise be used to remove any specific precursor identified as necessary for acrylamide formation.
- Leaching of components in the potato slices by the potato extract or the leaching stream occurs for those components for which a concentration gradient exists between the soluble matter in the potato slices and the potato extract or the leaching stream.
- the leaching may be accomplished selectively by a potato extract that is deficient in the acrylamide pre-cursor to be removed, but has concentration levels of other desirable soluble matter or solutes that are at or near equilibrium with the corresponding concentration levels in the potato slices.
- 'equilibrium' is meant one of two conditions: 1) the aqueous concentrations of a particular solute are substantially the same both in the extract and in the potatoes; or 2) the extract is saturated and cannot absorb any more of that particular solute.
- desirable soluble matter is
- CFLAY.00344PCT -21- PCT Application defined as any native soluble potato compound, other than reducing sugars, that is not an acrylamide pre- cursor.
- An example of selective leaching involves making the potato extract deficient in asparagine, and then contacting the raw potato slices with the asparagine-deficient potato extract to selectively leach asparagine out of the raw potato slices.
- the leaching is further enhanced by ultrasonically vibrating the potato extract while the potato extract is in contact with the potato slices.
- the potato extract or the leaching stream can be treated to remove the leached acrylamide pre-cursors so that the potato extract or the leaching stream can be recycled for continuous use in the leaching of more potato slices.
- Tests were run to ascertain the levels of asparagine in potato slices soaked in different solutions for different residence times. Twelve separate tests and a control test were perfo ⁇ ned. The control sample consisted of a fresh, unsoaked potato slice. The twelve tests consisted of soaking potato slices in four different solutions at three different residence times. The results are summarized in the Table 2 below.
- Each batch of potato extract started with about 800 grams of peeled potato slices and about 1500 mL of water placed in a blender and made into an unfiltered potato extract. The unfiltered extract was then vacuum filtered though a laboratory filter paper with a 20 to 25 micrometer pore size to remove solids.
- the filtered extract was further concentrated by repeating the steps in the above paragraph, but instead of adding about 800 grams of peeled potato slices to about 1500 mL of water, the 800 grams of potato slices were added to the filtered extract. This process was repeated several times to build-up the concentration of desirable soluble matter in the potato extract so as to minimize the amount of desirable soluble matter that was leached out of the potato slices.
- the filtered potato extract was next heated to a temperature of 120 0 F, About 340 microliters of asparaginase having 14,280 units/ml was added to 1500 mL of the potato extract to result in an asparagine-deficient potato extract having about 4844 units of asparaginase.
- levels of asparaginase can be used and in one embodiment, about 3,000 units to about 100,000 units of asparaginase are used per kilogram of total raw potato used to make the asparagine-deficient potato extract.
- Lower levels of asparaginase can be used, but more time may be required to achieve desired, lowered levels of asparagine in the asparagine-deficient
- one embodiment of the present invention comprises providing a potato extract deficient in asparagine and contacting potatoes such as sliced potatoes having a first concentration of asparagine with the asparagine-deficient potato extract to selectively leach asparagine from the potato slices.
- the potato slices can then be removed from the asparagine deficient potato extract, optionally rinsed and thermally processed. Separate tests have shown a relationship between the level of asparagine in a food product and the resultant level of acrylamide in that food product. Consequently, the resultant thermally processed potato slices with a reduced level of asparagine will have a reduced level of acrylamide.
- the potato extract is initially made from a potato to water ratio of between about 0.5 grams and about 2 grams of peeled potato is used per 1 mL of added solution. Higher potato to water ratios can make filtration more difficult. Thus, a lower ratio may be more desirable, especially if the potato extract is further concentrated by blending
- the potato extract disclosed above uses a ratio of 800 grams of peeled potato slices to 1500 mL of water or filtered extract, this ratio can be optimized. Added water creates some concentration gradient between desirable soluble matter such as reducing sugars and the potato extract. Consequently, in one embodiment, additives such as reducing sugars including fructose and glucose are added to the potato extract to further minimize the concentration gradient of desirable soluble matter. Further, the initial solution can comprise additives such as reducing sugars or other desirable soluble matter to further reduce the concentration gradient.
- the level of added water is minimized. In one embodiment, the level of water initially added is eliminated. Consequently, no water is added and the extract comprises macerated potato. In one embodiment, the macerated potato is vacuum filtered into a potato extract.
- the washing phase can be modified to include a continuous process for leaching acrylamide pre-cursors out of a continuous substantially untreated potato feed.
- a peeled potato slice is untreated.
- CFLAY.00344PCT -25- PCT Application operations are used to wash the potatoes and selectively extract the main pre-cursor, asparagine: 1) an extraction unit 1320 leaches asparagine out of an untreated potato feed 1310 and into an asparagine-deficient potato extract 1380; 2) a starch removal unit 1340 removes excess, unbound starch 1336; and 3) at least one asparagine removal unit 1350 removes asparagine from the de- starched, post-wash potato extract 1334, which is then recycled (as asparagine-deficient extract 1380) to continuously wash and leach the untreated potato feed 1310.
- treated potato slices 1312 then proceed to the next processing step, such as cooking or heating.
- a stream of peeled and sliced potatoes containing acrylamide pre-cursors (“untreated potatoes") 1310 enters an extraction unit 1320, similar to the prior ail washing step 23 of Figure 2, where the potatoes are contacted with a pre-cursor deficient potato extract 1380.
- the pre-cursor deficient potato extract 1380 comprises a stream of water containing all of the water-soluble potato components with the exception of the particular acrylamide pre-cursor to be removed from the untreated potato feed 1310.
- the pre-cursor deficient potato extract 1380 comprises an aqueous solution or suspension of all the water-soluble potato solids and compounds except asparagine.
- the potato extract generally includes water-soluble compounds such as reducing and non- reducing sugars, starches, and various amino acids.
- concentrations of water- soluble compounds in the pre-cursor deficient extract 1380, other than starch and asparagine, are either in or nearly in equilibrium with the corresponding concentrations of water-soluble compounds in the untreated potato feed 1310.
- concentrations of water-soluble compounds in the pre-cursor deficient extract 1380 are brought up to equilibrium levels by cycling a predetermined amount of pure water (distilled, de-ionized, or treated by reverse-osmosis) through the leaching system 1300 until enough water-soluble matter is leached
- CFLAY.00344PCT -26- PCT Application out of the untreated potato feed 1310 and into the extract 1322. Because the initial stream of potatoes entering the extraction unit are leached with water that is still relatively pure, a significant amount of desirable soluble matter is extracted out of those potatoes and into the cycling water. Some of those potatoes leached during start-up may result in lower quality potato chips after cooking, thus it may be desirable to simply discard those start-up potatoes after leaching. Similarly, it may also be desirable during start-up to use potatoes that would have otherwise been discarded because of an unacceptable size or shape. Again, those start-up potatoes may be discarded after washing if too much of the desired soluble compounds have been leached out.
- the prc-cursor deficient extract 1380 concentration levels (other than asparagine, excess starch, and undesirable impurities) to be in or nearly in equilibrium with the corresponding concentration levels in the untreated potato feed 1310 so that no concentration gradients draw desirable soluble matter out of the untreated potato feed 1310 during leaching.
- the potato slices are pre-washed to remove loose starch from the slices and reduce starch build-up in the extraction unit 1320.
- a heated pre-cursor deficient extract 1380 may be desirable to use. Because the solubility of asparagine in water increases with temperature, higher wash/leaching temperatures increase the amount of asparagine that can be leached with a given flow rate of pre-cursor
- CFLAY.00344PCT -27- PCT Application deficient extract 1380 For example, typical leaching solution temperatures can range from about 70 0 F to about 150 0 F. Further, the higher end of this temperature range (about 12O 0 F to about 150 0 F) can be more preferred when potato extract is the leachate because less desirable soluble matter is leached due to the preferential leaching of asparagine. [0073] Referring again to Figure 13a, as the asparagine-deficient potato extract 1380 contacts the untreated potatoes 1310 in the extraction unit 1320, asparagine and starch arc leached into the pre-cursor deficient extract 1380.
- the pre-cursor deficient extract 1380 contacts the untreated potatoes 1310 in a continuous, countercurrent fashion. It is well understood in the art that countercurrent flow, as compared to parallel flow, more effectively leaches a desired solute from a given solid.
- the extraction process is further enhanced by ultrasonically vibrating the pre-cursor deficient extract 1380 while it is in contact with the untreated potato feed 1310. High frequency, short amplitude vibrations help increase the rate of mass transfer by reducing the thickness of boundary layers around the potato pieces.
- a screw-type extractor comprises a
- CFLAY.00344PCT -28- PCT Application helix rotating inside a tube.
- the helix moves the solid to be leached in one direction along down the length of the tube while a countercurrent stream of extracting solvent flows along down the length of the tube in the opposite direction.
- Screw-type extractors for blanching potatoes are well known in the art, thus the construction and operation of screw-type extractors need not be described in detail herein.
- untreated potatoes 1310 enter a screw-type extraction unit 1320 and are then continuously moved along by a rotating spiral or helix that runs the length of the extraction unit 1320.
- the untreated potato feed 1310 can be divided and sent through several screw-type extraction units 1320 in parallel, then rejoined after exiting the extraction units.
- the pre-cursor deficient potato extract 1380 enters the unit at the end where the treated potatoes 1312 exit, flows through down the extraction unit 1320 in the opposite direction that the treated potato stream 1310 travels, and exits the unit where the untreated potatoes 1310 first enter the unit 1320, As the pre-cursor deficient extract 1380 flows through down the tube in this countercurrent fashion, the pre-cursor deficient extract 1380 is forced to contact the untreated potato feed 1310, and acrylamide pre-cursors and starch are leached from the untreated potatoes 1310 into the pre-cursor deficient extract 1380.
- the pre-cursor deficient extract 1380 extract can be pumped through the extraction unit 1320 at higher pressures and velocities to more effectively leach out the acrylamide pre-cursors.
- concentration levels of acrylamide pre-cursors, such as asparagine, remaining in the potatoes exiting the extraction unit 1320 depend upon various parameters, such as the residence time in the unit, the flow rates of both the untreated potato feed 1310 and the
- CFLAY.00344PCT -29- PCT Application pre-cursor deficient extract 1380, and the temperature of the pre-cursor deficient extract 1380. For example, if a lower final concentration of pre-cursors is desired in the treated potato stream 1312, the length of the extraction unit 1320 can be increased, thereby increasing residence time.
- Another embodiment of the extraction unit 1320 comprises a wash tank containing pre-cursor deficient potato extract 1380.
- an open- or wire-mesh type conveyor carries untreated potatoes 1310 into one end of the tank and immerses them in the pre-cursor deficient potato extract 1380 for a predetermined time and distance before bringing the leached potatoes 1312 out of the pre-cursor deficient extract 1380 at the opposite end of the wash tank.
- the pre-cursor deficient extract 1380 can be introduced into the tank at the end where the leached potatoes 1312 are removed, and the post- wash extract 1322 can be removed at the end where the untreated potato feed 1310 enters the tank.
- this particular embodiment of the acrylamide pre-cursor deficient extraction unit 1320 is easily implemented.
- the existing starch-removal wash tank can still be used with only a few minor changes.
- this type of extractor is that the relative velocity between the untreated potato feed 1310 and the pre-cursor deficient extract 1380, within the tank must be low enough to prevent the untreated potato feed 1310 from being forced off of the conveyor.
- this embodiment may not leach pre-cursors out a potato feed 1310 as quickly as screw-type extractors.
- Yet another embodiment of the extraction unit 1320 involves leaching by percolation of a pre-cursor deficient extract 1380 through moving beds of potato feed 1310.
- a pre-cursor deficient extract 1380 is leaching by percolation of a pre-cursor deficient extract 1380 through moving beds of potato feed 1310.
- One such type of extractor is the Bollman extractor, which comprises a revolving bucket elevator in
- CFLAY.00344PCT -30- PCT Application an enclosed casing.
- the revolving elevator has both a rising side and a declining side, and it also has separate sumps for the rising and declining sides in which extract collects.
- Bollman-type extractors have both countercurrent- and concurrent- flow sections: a countercurrent-flow section on the rising side and a concurrent-flow section on the declining side.
- untreated potatoes 1310 are placed into buckets at the top of the elevator on the declining side, and a prc-cursor deficient extract 1380 is introduced over the top buckets on the rising side. Extract that has percolated its way through the buckets on the rising side collects in the rising-side sump. The extract in the rising-side sump is pumped back to the top of the elevator and introduced over the buckets on the declining side. After reaching the declining-side sump, the post-wash extract 1322 is pumped out of the extraction unit 1320 and on to the next unit operation. Once the untreated potatoes 1310 have made their way down the declining side and up the rising side, they are removed from the top buckets on the rising side and transferred out of the extraction unit 1320.
- Percolation typically offers a greater extraction rate than immersion because there tends to be more mixing in percolation, due to a shorter boundary layer between the extract solvent and the solids.
- immersion extractors When compared to screw- type immersion extractors, there tends to be less mechanical damage to the flakes. Furthermore, there is less tendency for plugging.
- Yet another embodiment of the extraction unit 1320 comprises an inclined channel through which a potato pre-cursor deficient extract 1380 flows. While the pre-cursor
- Application deficient extract 1380 flows down the channel from its higher end to its lower end, an open- or wire-mesh type conveyor transports untreated potatoes 1310 up the channel and through the downward flowing pre-cursor deficient extract 1380.
- this wire- mesh type conveyor can be elevated slightly above the bottom of the channel so that the precursor deficient extract 1380 flows above, below, and around the untreated potatoes 1310 to be leached.
- the conveyor can comprise any food-grade mesh, chain, perforated, or other liquid- pervious structure made from a durable material, such as stainless steel or another type of metal, a ceramic, or a polymer-based material.
- a durable material such as stainless steel or another type of metal, a ceramic, or a polymer-based material.
- the pre-cursor deficient extract 1380 enters the top of the last extraction unit and makes its way down each unit's inclined channel until it reaches the bottom of the first extraction unit. By that time, the post-wash extract 1322 has pre-cursor concentrations near those of the untreated potatoes 1310.
- the starch removal unit 1340 comprises a cyclone for centrifugally removing starch. Because starch particles have a higher density than most other water-soluble molecules in the post- wash extract 1322, the spinning cyclone 1340 causes the heavier-than- water starch particles to separate from the extract 1322 at the cyclone walls and then be directed out of the cyclone 1340 as a slurry or paste 1336. The de-starched, post-wash extract 1334 then leaves the cyclone 1340 and is transferred to at least one pre-cursor removal unit 1350. While the preferred embodiment of the
- Application starch removal unit 1340 comprises a cyclone, other types of equipment and methods can be used to remove starch. For example, starch might also be removed by filtration, batch sedimentation, or flocculation. Note, however, that the starch removal unit 1340 is not necessary if starch is eliminated from the untreated potato feed 1310 prior to the extraction unit 1320. [0079] In the third unit operation, the de-starched, post-wash extract 1334 enters at least one pre-cursor removal unit 1350 where the acryf amide pre-cursors are removed.
- each pre-cursor removal unit 1350 comprises either a packed-tower- or baffle- tower-type resin column such as an ion exchange resin comprising adsorption materials that selectively bind with the particular acrylamide pre-cursor to be removed.
- each resin column comprises an immobilized enzyme such as asparaginase.
- the pre-cursor removal unit 1350 comprises a column having an asparaginase-coated resin or a resin that selectively removes asparagine from solution by, for example, binding with the asparagine.
- the asparagine in the extract reacts with the resin and is converted in ammonia and aspartic acid.
- the de-starched, post- wash extract 1334 is introduced at the top of each column and percolates downward in a circuitous path on its way to the bottom.
- asparagine is selectively removed by the resin.
- a significant amount of asparagine is removed from the de-starched, post- wash extract 1334 by the time it reaches the bottom of the resin column.
- a significant amount is removed when at least 50% of the asparagine concentration is removed from the de-starched, post-wash extract 1334.
- CFLAY.00344PCT -33- PCT Application asparagine-deficient extract 1380 is then removed from the bottom of the resin column and recycled to the extraction unit 1320 for another round of leaching.
- Several such columns can be operated in series to further reduce the asparagine concentrations in the de-starched, post- wash extract 1334, Furthermore, two or more columns or series of columns can be run in parallel in order to allow one column (or series of columns) to be taken off-line for regeneration without halting the continuous leaching process 1300.
- Figure 13b illustrates the regeneration process if required for a resin column that selectively removes asparagine from the solution by binding with the asparagine.
- a regenerating solution 1360 is sent through the saturated column 1350 to free the asparagine from the surface of the resin.
- the asparagine-laden regenerating solution 1362 exiting the off-line column 1350 can be discarded, or it can be further processed to isolate the asparagine and recycle the regenerating solution 1360.
- the extraction unit operation 1320 can be split into two separate unit operations.
- the first of these unit operations would involve a washing step similar to the prior art washing step 23 shown in Figure 2. This washing step would involve water and would be for the purpose of removing excess starch from the surface of the potato slices.
- the thus-washed potato slices would then proceed to an extractor unit 1320 to be treated
- FIG. 14 depicts another embodiment of the invention in which a non-selective leaching system 1400 is used to wash raw potato pieces and remove asparagine.
- This nonselective leaching system 1400 comprises four main unit operations: 1) an extraction unit 1420 non-selectively leaches water-soluble compounds, including asparagine, out of a potato feed stream 1410 and into a pre-cursor deficient extract 1460; 2) a starch removal unit 1440 removes excess, unbound starch 1466 from the resulting post-wash extract 1462; 3) at least one asparagine removal unit 1450 removes asparagine from the de-starched, post-wash potato extract 1464; and 4) an absorption/replenishing unit 1480 replenishes the treated potatoes 1412 with some of the previously extracted water-soluble compounds.
- the extraction unit 1420, starch removal unit 1440, and pre-cursor removal unit(s) 1450 shown in Figure 14 are constructed and operated in a manner similar to that described with respect to the extraction unit 1320, starch removal unit 1340, and pre-cursor removal unit(s) 1350 shown in Figure 13a.
- the non-selective leaching system 1400 depicted in Figure 14 does not require that the untreated potato feed 1410 in the extraction unit 1420 be leached with a potato extract.
- a continuous stream untreated potato feed 1410 entering the extraction unit 1420 of Figure 14 can instead be leached with pure water (distilled, de-ionizcd, or treated by reverse-osmosis) as the pre-cursor deficient extract 1460. Furthermore, as will be understood by reviewing the entire process, the spent potato extract 1472 leaving the last unit operation 1480 can be discarded and
- CFLAY.00344PCT -35- PCT Application does not have to be recycled to the extraction unit 1420.
- the potato spent extract 1472 can be recycled if desired.
- a continuous stream of raw potatoes containing acrylamide pre-cursors (“untreated potatoes") 1410 flow from one end of an extraction unit 1420 to the other end while a pre-cursor deficient extract 1460 flows through the extraction unit 1420 in the opposite direction, countercurrent to the flow of untreated potatoes 1410.
- the pre-cursor deficient extract 1460 typically comprises pure water but can alternatively comprise spent potato extract 1472 exiting from an absorption/replenishing unit 1480 or a combination of both pure water and spent extract 1472.
- the extraction unit 1420 shown in Figure 14 non-selectively draws any water-soluble compounds out of an untreated potato feed 1410. Because the pre-cursor deficient extract 1460 contains little or none of the water-soluble compounds contained in the untreated potato feed 1410, such as reducing and non-reducing sugars, starch, and asparagine, the concentration gradients between the untreated potato feed 1430 and the pre-cursor deficient extract 1460 cause the water-soluble compounds in the untreated potato feed 1410 to diffuse into the pre-cursor deficient extract 1460. After washing and leaching, the treated potatoes 1412 exit the extraction unit 1420 and proceed to an absorption/replenishing unit 1480, while the post- wash extract 1462 exits the extraction unit 1420 and proceeds to a starch removal unit 1440.
- the post- wash extract 1462 which now contains starch, asparagine, reducing and non-reducing sugars, and other water-soluble compounds, enters a starch removal unit 1440.
- This unit is constructed
- the starch removal unit 1440 separates the starch particles from the post-wash extract 1462, and the starch exits the unit as a slurry or paste 1466.
- the de-starched, post- wash extract 1464 then leaves the starch removal unit 1440 and is transferred to at least one pre-cursor removal unit 1450.
- At least one pre-cursor removal unit 1450 removes asparagine in the same manner as described above with respect to the pre-cursor removal unit(s) 1350 in Figure 13a.
- each pre-cursor removal unit 1450 in Figure 14 is regenerated the same way as each pre-cursor removal unit 1350 shown in Figure 136 is regenerated, if necessary.
- the resulting pre-cursor deficient replenishing extract 1470 exits the precursor removal unit(s) 1450 and proceeds to an absorption/replenishing unit 1480.
- the fourth unit operation of the non-selective leaching system 1400 returns previously leached water-soluble compounds to the treated potatoes 1412.
- An absorption/replenishing unit 1480 is used for this purpose and comprises any one of the apparatuses discussed in the several embodiments of the extraction unit 1320 shown in Figure 13a.
- the absorption/replenishing unit 1480 can comprise a screw-type extractor, a wash-tank type extractor, or a Bollman-type percolation extractor. Although most often used to extract solute from solids into a leaching solvent, these apparatuses can also be used to accomplish the reverse — diffuse solute from solution into solids. Such equipment merely enhances mass transfer between solid and liquid phases.
- one embodiment of the absorption/replenishing unit 1480 comprises a screw-type extractor. The treated potatoes 1412 from the extraction unit 1420 enter one end of the screw-type extractor 1480 and encounter a
- CFLAY.00344PCT -37- PCT Application countercurrent flow of pre-cursor deficient replenishing extract 1470. Because the entering precursor deficient replenishing extract 1470 has a relatively high concentration of water-soluble compounds (mainly sugars), and the treated potatoes 1412 have a relatively low concentration of water-soluble compounds, the water-soluble compounds diffuse from the pre-cursor deficient replenishing extract 1470 back into the treated potatoes 1412. Thus, the water-soluble solute concentrations in the treated potatoes 1412 increase as the treated potatoes 1412 progress through the absorption/replenishing unit 1480, and the solute concentrations in the pre-cursor deficient replenishing extract 1470 decrease as the pre-cursor deficient replenishing extract 1470 progresses through the unit 1480.
- water-soluble solute concentrations in the treated potatoes 1412 increase as the treated potatoes 1412 progress through the absorption/replenishing unit 1480, and the solute concentrations in the pre-cursor deficient replenishing extract 1470 decrease as the pre-cursor deficient replenishing extract 1470 progresses through
- the spent extract 1472 exiting the absorption/replenishing unit can be discarded, or it can be recycled as part of the pre-cursor deficient extract 1460 in the extraction unit 1420.
- the replenished potatoes 1414 exit the unit 1480 with water-soluble solute levels greater than those in the treated potato stream 1412 but lower than the initial levels in the raw, untreated potatoes 1410.
- the non-selective leaching system will, over time, operate much like the selective leaching system shown in Figure 13a. As steady-state is approached, the concentrations of water-soluble matter other than asparagine and starch will increase in the precursor deficient extract 1460, and less matter will be leached out of the untreated potato feed 1410. At steady-state, only asparagine and starch are extracted out of the potato feed 1420 and into the pre-cursor deficient extract 1460, rendering the absorption/replenishing 1480 unit superfluous.
- another embodiment of the invention involves using the non-selective leaching system 1400 only during start-up, recycling the spent extract 1472 as pre-cursor deficient extract 1460 in the extraction unit 1420, and then transforming the non-selective
- the removal of pre-cursors occurs through leaching by prolonging the blanching step that involves treating whole or sliced potatoes 1410 with hot water 1460 from 100 0 F to 150 0 F and more preferably about 120 0 F to 15O 0 F with soaking times ranging from about 1 minutes to about 5 minutes and more preferably about 3 minutes to about 5 minutes.
- the temperature/time relation is important because lower temperatures do not remove sufficient amounts of precursors and higher temperatures remove all components too readily.
- the potato slices are blanched in a solution having one or more desirable soluble material additives including but not limited to reducing sugars such as glucose and fructose.
- the blanching step in accordance with one embodiment of the present invention removes amounts of asparagine to effect up to 95% reduction of acryl amide formation.
- the de- starched, post-wash extract 1464 which contains leached out components from the blanching step is then cooled to prevent the enzyme from becoming inactivated and mixed with enzyme asparaginase with a concentration ranging from 500 to 100,000 units and more preferably between about 3000 to about 100,000 units per 1.4 liters of solution for 5 to 20 minutes.
- the dc-starched, post-wash extract 1464 is cooled to a temperature of less than about 70 0 C (158°F) to prevent destruction of asparaginase.
- the solution is cooled to a temperature of between about ambient and about 100 0 F.
- the asparaginase removes a
- the blanched treated slices 1412 are replenished with desirable soluble matter with the enzyme treated pre-cursor deficient replenishing extract 1470 to infuse back into the slices the rest of the components (without asparagine) to produce replenished potatoes 1414.
- the infusion step that occurs in the absorption/replenishing unit 1480 may involve either various temperature/time treatments, possibly higher pressures or vacuum.
- the slices, after these treatments, are fried per normal frying temperature and times to produce chips.
- FIG. 15 is a schematic diagram of a system and method adding an enzyme 1555 to the de-starched, post-wash extract 1574 to provide an acrylamide pre-cursor deficient solution 1580, contacting raw food or untreated potato pieces 1510 having a native concentration of asparagine in the acrylamide pre-cursor deficient solution 1580 to preferentially leach out the acrylamide pre-cursor from the untreated food pieces 1510 such that the raw food piece has a reduced concentration of asparagine that is less than the native concentration, and removing the treated food pieces 1512 from the extraction unit 1520.
- the washing phase can be modified to include a continuous process for leaching acrylamide pre-cursors out of a continuous potato feed 1510.
- five main unit operations are used to wash the potatoes and selectively extract the main pre-cursor, asparagine: 1) a blender 1530 makes a potato extract 1532; 2) a starch removal unit 1540 removes excess, unbound starch and potato solids 1546; 3) at least one asparagine removal unit 1550 removes asparagine from the de-starched, post- wash potato extract 1534, which is then recycled (as asparagine-deficient extract 1552) to continuously wash and leach the untreated potato feed 1510; and 4) at least one reaction product removal unit 1560 can be used to avoid build-up of reaction products such as ammonia and aspartic acid that can result from reacting asparagine with asparaginase in the pre-cursor removal unit 1550; and 5) an extraction unit 1520 leaches asparagine out of an untreated potato feed stream 1510 and into an asparagine-deficient potato extract 1580.
- a blender 1530 makes a potato extract 1532
- a starch removal unit 1540 remove
- the amount of water 1516 used to make the extract 1532 is preferably minimized.
- the extract comprises between about 300 grams to about 1,000 grams of potato per 1.4 liters of water. In one embodiment, the water comprises between about 500 units and about 100,000 units per 1.4 liters.
- a heated asparagine-deficient extract 1580 may be desirable to use. Because the solubility of asparagine in water increases with temperature, higher wash/leaching temperatures increase the amount of asparagine that can be leached with a given flow rate of extract. For example, typical leaching solution temperatures ranging from about 100 0 F - about 150 0 F can be used.
- the de-starched, post-wash extract 1534 enters at least one pre-cursor removal unit 1550 where the acrylamide pre-cursors are removed.
- the pre-cursor removal unit 1550 comprises one or more asparaginase 1555 injection ports.
- the pre-cursor removal unit 1550 comprises a continuous mixer.
- Sufficient asparaginase 1555 should be added such that the asparagine concentration in the asparagine-deficient exit stream 1552 comprises less than about 50% and more preferably less than about 90% of the asparagine concentration of the de-starched post- wash extract 1534. .
- the asparagine-deficient exit stream 1552 will have reaction products, namely ammonia and aspartic acid as a result of the asparaginase reacting with the asparagine. It is desirable in a continuous process to remove some or all of the reaction products prior to entering the extraction unit 1520 to maintain a favorable preferential or selective asparagine driving force out of the untreated potatoes 1510 and minimize any undesirable collateral effect such reaction products may have on the quality and characteristics of the treated potatoes 1512 exiting the extraction unit 1520. Consequently, several techniques can be used alone or in conjunction to lower the concentration of reaction products in the asparagine-deficient extract 1580 entering the extraction unit 1520.
- an amount 1558 of the asparagine-deficient exit stream ⁇ 552 is removed from the leaching system 1500 and can be routed to a waste water stream.
- the potato extract 1532 make-up stream entering the leaching system 1500 is proportional or equal to the amount 1558 of asparagine-def ⁇ cient extract that exits the leaching system 1500.
- the asparagine-deficient exit stream 1552 is divided into a by- pass stream 1556 and a slip stream 1554 that is routed to one or more reaction product removal units.
- an ammonia removal unit 1560 preferentially removes ammonia 1562 from the asparagine-deficient slip stream 1554.
- the slip stream 1554 can comprise about 0% to about 100% of the flow of the asparagine-deficient extract stream 1552.
- the slip stream 1554 can be set to operate intermittently such that all or a portion of the asparagine-deficient extract stream 1552 is routed to one or more reaction product removal units 1560 for a selected amount of time. For example, for every hour of operation of the leaching system 1500, the asparagine- deficient slip stream 1554 may operate for about 30 consecutive or intermittent minutes and may process all or portion of the asparagine-deficient extract stream 1552.
- the ammonia removal unit 1560 can remove ammonia by one or more ammonia removal methods alone or in combination.
- the ammonia removal method can be selected from heating the stream, reducing the pressure of the stream, bubbling a gas such as air, steam, or nitrogen through the stream, adjusting the pH of the stream, and using an ion exchange resin to selectively remove ammonia from the stream.
- ammonia removal unit 1560 can comprise two units; one unit for regeneration and one unit for ammonia removal.
- an aspartic acid removal unit 1570 can remove aspartic acid 1572 by one or more aspartic acid removal methods alone or in combination.
- the aspartic acid removal method can be selected from using an ion exchange resin to selectively remove aspartic acid from the stream, filtering with an ultra- filtration membrane, electrophoresis, and adjusting the pH of the stream.
- An ion exchange resin in the ammonia removal unit 1560 and/or the aspartic acid removal unit 1570 can be used in a packed bed.
- the ion exchange resin can be used in a batch-continuous operation where resin is placed into a vat and mixed with the slip stream 1554 and the resin absorbs the reaction pre-cursors and the resin can be removed and regenerated.
- the pH of the slip stream 1554 is lowered to precipitate aspartic acid.
- the precipitate can then be removed by centrifuge, hydroclone, filtration, or other suitable means.
- the pH is adjusted to a range of between about 4 and about 6 and more preferably between about 4,5 and about 5.5.
- An ultrafiltration membrane can be used in alone or in combination with a pH adjustment to filter out the aspartic acid.
- the resultant purified pre-cursor deficient potato extract 1574 having been routed through the ammonia removal unit 1560 and/or the aspartic acid removal unit 1570 will have fewer reaction products or by-products (e.g., less ammonia and/or aspartic acid) than the asparagine deficient exit stream 1552.
- the purified pre-cursor deficient potato extract 1574 can mixed with the by-pass stream 1556 and become the asparagine-deficient potato extract 1580.
- a stream of peeled and sliced potatoes containing acrylamide pre-cursors (“untreated potatoes") 1510 enters an extraction unit 1520, similar to the prior art washing step 23 of Figure 2, where the potatoes are contacted with a pre-cursor deficient potato extract 1580.
- the asparagine-deficient potato extract 1580 comprises a stream of water containing all of the water-soluble potato components with the exception of the particular acrylamide pre-cursor Io be removed from the untreated potato feed 1510.
- the asparagine-deficient potato extract 1580 comprises an aqueous solution or suspension of all the water-soluble potato solids and compounds except asparagine.
- the potato extract generally includes water-soluble compounds such as reducing and non-reducing sugars, starches, and various amino acids.
- concentrations of water-soluble compounds in the asparagine-deficient extract 1580, other than starch and asparagine are either in or nearly in equilibrium with the corresponding concentrations of water-soluble compounds in the untreated potato feed 1510.
- FIG. 5 focuses on another aspect of unit operations and shows the effect of decreasing moisture level in the chip during the cooking stage.
- the cooking step 24 is a unit operation that typically involves cooking sliced potato chips in a continuous oil fryer at high temperatures.
- the graph thereon reflects on the horizontal or x- axis the moisture level of the final chip product.
- the vertical or y- axis is again labeled in ppb of acrylamide ("AA”) found in the final product.
- a number of data points are then plotted showing a percent moisture versus the acrylamide level of the final chip.
- the line plots 51, 52 are curve-fitted to the data points in order to establish a trend.
- the first line plot 51 relates to the 149 0 C (300 0 F) frying temperature data points.
- the second line 52 relates to the data points plotted for the 178°C (353 0 F) frying temperature.
- acrylamide levels remain very low at chip moisture levels above about 3% moisture by weight regardless of frying temperature.
- Figure 5 shows the relationship between acrylamide levels and moisture content in fried potato slices
- Figure 6 depicts the same relationship in baked potato chip products made from a dry mix.
- the vertical axis of the graph in Figure 6 shows acrylamide concentrations, while the horizontal axis shows moisture levels by weight. While the acrylamide concentrations tend to be higher in baked potato chip products than in fried potato slices, Figures 5 and 6 both show that the acrylamide concentrations remain fairly low in cooking potato products until the moisture level falls below about 3%.
- Figures 5 and 6 What is made apparent by Figures 5 and 6 is that acrylamide levels in potato chips cooked in a typical fryer increase rather dramatically once the moisture level falls below 3% moisture by weight, at which point it seems there is not enough moisture left to keep the product temperature below an acrylamide formation temperature.
- Figure 5 illustrates that the level of acrylamide found in the final product is relatively low when the moisture level of the chip during the cooking unit operation is 3% by weight or greater, regardless of the exposure to high-temperature cooking environments.
- Figures 5 and 6 demonstrate that moisture level is a useful additional parameter in a unit operation that can be adjusted for the reduction of acrylamide formation in the final product.
- the moisture level in a finished potato chip should ideally be below about 2%, and preferably between about 1.3 and 1.4%. Anything higher than 2%, and even higher than 1.4% can lead to staling and microbial spoilage issues in the packaged product, as well as organoleptic consequences, for example, taste, texture, etc. However, changes in color, taste, and consistency of the final product can be adjusted by various means. In addition, it may be possible to counter the consequences of finishing the food product with a higher moisture content by adjusting various factors in the pre-packaging step, such as extending fryer hoods,
- a further unit operation comprises finishing the food product as it emerges from its final cooking step at a moisture content, for example, at about 1.4% by weight, about 1.6% by weight, about 1.8% by weight and about 2% by weight, or at any % moisture weight between 1.4% and 2%.
- CFLAY.00344PCT -50- PCT Application acrylamide formation temperature It is important to keep in mind, though, that not all portions of a cooking product share the same internal temperature. French fries, for example, can be fairly thick when compared to potato slices and thus tend to have a larger moisture gradient between the inner and outer portions of the product. Consequently, it is possible for a French fry being cooked to have a fairly high surface temperature even though its interior moisture content is high. In contrast, a potato slice is thinner and tends to have more consistent moisture levels throughout the slice during cooking. Thus, at least for thin products such as potato slices or fabricated potato pieces, moisture level can still be a good gauge of its internal temperature.
- one element of this invention involves dividing the cooking unit operation (the fourth unit operation 24 shown in Figure 2) into at least two separate heating steps.
- a first heating step occurs at elevated temperatures to reduce the moisture level to some point near but above 3% by weight.
- the product is then finished to the desired moisture level of about 1 -2% by weight, but preferably about 1.4% by weight, with a lower-temperature cooking step having a temperature below about 12O 0 C (250 0 F).
- a lower-temperature cooking step having a temperature below about 12O 0 C (250 0 F).
- the process modifications described herein are not limited to prior art processes for cooking potato slices such as the one disclosed in Figure 2. These modifications are also applicable in processes for making fabricated products derived from potato, corn, wheat, rye, rice, oats, millet, and other starch- based grains. For example, these process modifications can be used to reduce acrylamide
- modified cooking step and “modified cooking unit operation” are meant to include not only Figure 2's prior art method for cooking potato slices but also prior art methods for preparing other food products in which it is desirable to reduce acryiamide formation.
- potato-based pieces is meant to include both raw potato slices and fabricated potato pieces derived from potato starch or dough.
- Each heating step can be accomplished using various heating methods.
- the first heating step can comprise atmospheric frying, vacuum frying, microwave- assisted frying, or baking.
- the first heating step can alternatively comprise any other method for cooking the product and lowering its moisture level with primary consideration given to production efficiencies such as residence time, energy costs, equipment capital costs and available floor space.
- the first heating step is often called "par- frying," as such frying only partially cooks the product until its moisture content is lowered to some point near but above 3% by weight.
- the second heating step can comprise vacuum frying, low temperature oven drying, vacuum oven drying, or any method of cooking that maintains cooking temperatures required by the second heating step.
- a potato-chip-making process using fresh chipping potatoes combines traditional peeling, slicing, and washing steps with a modified cooking unit operation involving par-frying at about 165 to about 182 0 C (330-360 0 F) for about 1-3 minutes, followed by oven- drying below about 120 0 C (25O 0 F) until the chip moisture level is reduced to about 1.4% by weight.
- acrylamide levels below 130 ppb are achieved. This preferred embodiment achieves a balance between a high level of acrylamide reduction with an acceptable change in product quality associated with the necessary process modifications.
- other embodiments are possible.
- Figures Ia, Ib, and 8 show various examples of combinations of washing modifications comprising contacting with an aqueous solution and cooking modifications that reduce final acrylamide levels from those levels resulting from the prior art methods. For example, a final acrylamide level of more than 300 ppb is reduced to less than 100 ppb.
- Figures Ia, Ib, and 8 involve embodiments for processing raw potato slices, the modified washing methods used in those embodiments can also apply to other types of raw foods in which acrylamide reduction is desirable, such as sweet potatoes, yams, and plantains.
- the cooking modifications used in those embodiments can also apply to other fried food products such as fried tortillas, fried plantains, fried sweet potatoes, and fried yams.
- Figure Ia depicts the resulting acrylamide levels of potato chips made from combining several different embodiments of a modified washing step comprising contacting with one particular embodiment of a modified cooking step.
- the modified cooking step of Figure Ia comprises partially frying ("par frying") potato slices at about 178 0 C (353°F) for approximately one to three minutes in a first heating step, then oven-drying the potato slices at about 12O 0 C
- the vertical or y- axis of the graph in Figure Ia shows acrylamide concentrations in ppb, while the horizontal or x- axis is labeled to show the parameters of each embodiment of the modified washing step comprising contacting the potato slices with an aqueous solution.
- Each data point shows a pair of vertical bars: the left bar represents acrylamide concentrations after contacting and par-frying while the right bar represents acrylamide concentrations after oven-drying.
- Reading left to right, the first data point 71 of Figure Ia like that of Figures 3 and 4, is a base sample involving a two to three minute water wash at ambient temperature, after which the sample is then atmospherically fried to roughly 1.3% moisture by weight.
- the second data point 72 is like the first except the sample is fried to about 1.0% moisture. Note that the first and second samples 71, 72 developed about 320 ppb and 630 ppb of acrylamide, respectively.
- the third data point 73 involves the same two to three minute ambient water wash, but the sample is then par fried to slightly above 3% moisture and oven-dried to about 1.3% moisture. The left and right bars show that the sample exited the par-frying step with a relatively low acrylamide concentration of about 65 ppb and gained less than 15 ppb in the oven-drying step.
- the fourth data point 74 involves an aqueous solution comprising water contacting the potato slices for a five minute contact time at about 6O 0 C (14O 0 F), followed by the par-frying and oven-drying steps of the modified cooking unit operation. This five-minute, 6O 0 C (14O 0 F)
- CFLAY.00344PCT -55- PCT Application step significantly reduces acrylamide levels.
- Figure 4 shows that frying in a traditional manner to a standardized 1.32% moisture level by weight should result in acrylamide concentrations ranging from slightly above 100 ppb to over 400 ppb
- Figure 8 shows that par- frying and oven-drying to the same standardized moisture level should result in significantly lower acrylamide concentrations under 100 ppb.
- the second data point 84 of Figure 8 shows that the final acrylamide level can be further reduced to less than about 40 ppb when a similar 5-minute, about 60 0 C (14O 0 F) contacting step is followed by a modified cooking unit operation involving par- frying and oven-drying.
- Figure 9 shows the dramatic increase in final acrylamide concentrations that results from using an oven-drying temperature above about 12O 0 C (250 0 F).
- the test samples were contacted and then par- fried in the same manner as in Figure 7b, but the samples were then oven-dried at about 176 0 C (35O 0 F) rather than about 120 0 C (250 0 F).
- the final acrylamide concentrations of the test samples were then normalized to show the expected acrylamide levels upon reaching about 0.76% by weight (which is the final moisture content that was reached in the base-point/standard two-to-three minute water wash shown as the first data point). Comparing the second data point 74 of Figure Ib with the second data point 94 of
- Another test sample (not shown) was washed to remove surface starch, par-fried at about 176 0 C (35O 0 F) to a moisture content of between about 3-5% by weight, and then dried in a commercial Wenger oven at about 132 0 C (27O 0 F) to a final moisture content of about 1.3% by weight, resulting in an acrylamide level of about 270 ppb.
- This principle applies not only to raw potato slices but also to other raw foods, such as yams and plantains, and fabricated products derived from potato, com, barley, wheat, rye, rice, oats, millet, and other starch-based grains.
- FIG. 10 charts the results and operating conditions of yet another embodiment in which potato slices were washed, par-fried, and then oven-dried.
- a control sample 101 was processed in a manner similar to that described with respect to the base samples 71 , 72 shown in Figure Ia. After about a 20-30 second ambient-temperature water wash, followed by briefly contacting the potato slices with a dilute (3 - 5%) solution of sodium chloride for a few seconds, a control sample 101 of 1.45 mm thick slices of peeled Henries chipping potatoes was par-fried in oil having an initial temperature of about 179°C (354 0 F) for approximately three minutes to 1.4% moisture by weight.
- the control sample 101 had an acrylamide concentration of 640 ppb, similar to the 630 ppb produced in the second base sample 72 shown in Figure Ia.
- the test sample 101 had an acrylamide concentration of 640 ppb, similar to the 630 ppb produced in the second base sample 72 shown in Figure Ia.
- CFLAY.00344PCT -57- PCT Application sample 102 was similarly washed and contacted like the control sample 101. Using a large commercial fryer, the test sample 102 was then par-fried in oil having an initial temperature of about 174°C (345°F) for about three minutes until the moisture content decreased to 2.5% by weight. The par- fried test sample 102 was then finish-dried for about six minutes using an oven at about 1 10 0 C (23O 0 F) until the moisture level decreased to 1.4% by weight. Cooking in this manner produced product with a reduced acryl amide concentration of 160 ppb, which is roughly 25% of the acrylamide concentration of the control sample 101.
- potato slices underwent a standard wash procedure were par- fried to about 3-5% moisture by weight, and then oven-dried to less than about 2% moisture by weight.
- a control sample was washed and then fried at about 179°C (354°F) to a finished moisture content of about 1.3% by weight, resulting in an acrylamide level of 380 ppb.
- par- frying test samples at about 179°C (354°F) to a moisture content of between about 3 to about 5% resulted in acrylamide levels of approximately 64 ppb.
- the par- fried product was then dried in a commercial Wenger oven at various temperatures.
- potato slices can be par- fried at about 179°C (354°F) to a moisture content of between 3-5% and then oven-dried at about 100°C (212 0 F) under atmospheric or slightly below atmospheric pressure without increasing the acrylamide levels beyond what is formed in the par-frying operation.
- potato slices can be removed from the par- fry step with moisture levels as high as 10% by weight, but removing the product too soon can affect the final texture of the product.
- this method is not limited to raw potato slices and can be applied to other fried food products such as fried tortillas, fried plantains, fried sweet potatoes, and fried yams.
- the advantage of par-frying and then oven-drying at about 100 0 C (212°F) is that the cooking unit operation alone can be modified to significantly reduce acrylamide formation from above 300 ppb to less than about 70 ppb; the standard peeling, slicing, and washing steps need not be modified.
- oven-drying it is also possible to conduct the oven-drying under vacuum in order to enhance moisture removal.
- oven-drying under vacuum less time is required to dry the product to the desired final moisture content.
- oven-drying at or near 100 0 C (212 0 F) does not cause any measurable increase in acrylamide levels
- oven-drying at that temperature takes a relatively long time to dry the product.
- vacuum oven-drying helps decrease the amount of time it takes for product to dry. It also helps decrease the amount of time the product is exposed to acryl ami de-forming temperatures, should higher oven-drying temperatures be used.
- Figures 7c, 76, 8, and 10 depicted test results from combining one particular embodiment of a modified cooking unit operation with several different embodiments of a modified washing unit operation comprising a contacting step
- other embodiments and combinations are possible.
- the various different contacting steps shown in those figures can instead be followed by a different modified cooking unit operation.
- an improved method for reducing acrylamide formation can simply utilize a modified cooking unit operation without modifying any of the other unit operations.
- the second of the two heating steps of a modified cooking unit operation comprises vacuum finish-frying rather than atmospheric frying.
- finish-frying under vacuum the partially fried or cooked product emerging from the first heating step can continue to be fried, but at a temperature too low to form significant amounts of acrylamide.
- the vacuum pressure should be such that frying occurs below about 120 0 C (250 0 F).
- Such vacuum finish-frying can also be applied to other fried food products such as those derived from potato, com masa, barley, wheat, rice, oats, millet, and other starch-based grains.
- FIG 11 charts the results and operating conditions of several examples of a modified cooking unit operation involving par- frying followed by vacuum finish-frying.
- control 1 10 and test samples 1 1 1, 1 12, 113, 114 Hermes variety of chipping potatoes were peeled, sliced to about 1.35 mm thickness, and subjected to a standard 20-to-30-second ambient- temperature water wash. After washing, the control sample 110 was filed at atmospheric pressure in oil having an initial temperature of about 177°C (351 0 F) for about 2.5 minutes to a moisture level of 0.83% by weight, producing an acrylamide concentration of 370 ppb.
- Tests 1-4 all of the test samples 1 1 1, 1 12, 1 13, 114 were atmospheric par-fried at about 177 0 C (351 0 F) and vacuum finish-fried at about 120 0 C (248°F) and 100 millibars, but each was par-fried and vacuum finish-fried for different lengths of time.
- Test 1 111, 220 ppb of acrylamide were found in the test sample after washing, atmospheric par-frying for about 100 seconds to 3% moisture by weight, and vacuum finish-frying for 44 seconds to about 0.7% moisture by weight.
- the results of Tests 2-4 1 12, 113, 114 show that acrylamide levels in the final product dramatically decrease when par-frying is stopped, and vacuum finish-drying is commenced, before the moisture content decreases to 3% by weight.
- Tests 2-4 112, 113, 114 all produced final acrylamide concentrations below 50 ppb.
- Test 4 114 an acrylamide level of only 13 ppb
- CFLAY.00344PCT -60- PCT Application was achieved by par-frying to 10% moisture by weight, then vacuum-frying to about 1% moisture by weight. As can be seen from the data, partially frying slices to higher moisture content before they are low-temperature vacuum finish-fried dramatically lowers final acrylamide concentrations. This method can also be used to reduce the final acrylamide concentrations in other fried products such as fried tortillas, fried plantains, fried sweet potatoes, and fried yams.
- the advantages of vacuum finish-frying after par-frying to about 3-10% moisture by weight are that the final stages of cooking can be completed at low temperatures without affecting product texture, and its effectiveness at reducing acrylamide formation can eliminate the need for a modified washing step comprising contacting the product with an aqueous solution.
- vacuum finish-frying also allows for the final stages of cooking to be completed at temperatures higher than those that can be used when not frying under a vacuum, while still providing a reduced acrylamide concentration in the finished product. It is noted that the vacuum finish-fried product had a lighter color than the control sample, and transferring cooking product from the par- frying operation to the vacuum finish-frying unit at higher moisture levels may impart a bland flavor to the product. It should be kept in mind that the capital cost of vacuum finish- frying equipment may be greater than that of oven-drying equipment.
- vacuum par-frying can be used in the first of the two heating steps of a modified cooking unit operation.
- a modified cooking unit operation involves vacuum par- frying to a moisture content near but above the threshold moisture level of 3-4% by weight, then oven-drying at no greater than about 12O 0 C (250 0 F) to completion.
- the product can be fried at lower temperature, thus producing less acrylamide.
- oven-drying at or below about 12O 0 C (250 0 F) ensures
- another embodiment of the invention comprises a modified cooking unit operation with a higher-temperature first baking step and a lower- temperature second baking step.
- the product is first baked at higher temperature (above about 12O 0 C (25O 0 F)) until its moisture content is reduced to about 4% to about 10% by weight.
- the product is then oven-dried (finish- dried or baked) at a temperature no greater than about 12O 0 C (25O 0 F) until the desired moisture level, typically about 1% to about 3% by weight, is attained.
- a convection oven can be used in the higher- temperature first heating step to reduce product moisture content to about 10% by weight.
- the oven may be divided into four heating zones in which the temperature is highest in the first zone and gradually decreases through the remaining three zones.
- a downdraft. single zone, convection oven may be used in the lower-temperature second heating step to complete the cooking process.
- Other types of ovens can be used for the two heating steps of this embodiment.
- the lower-temperature second heating step of this particular embodiment like that of the embodiments involving par- frying followed by oven- drying, can be performed at about 100 0 C (212 0 F) and slightly below atmospheric pressure so that little to no additional acrylamide is formed after the higher-temperature first heating step.
- CFLAY.00344PCT -62- PCT Application [0134] In tests using one example embodiment that involved a higher-temperature first baking step and a lower- temperature second baking step, fabricated potato pieces were first baked at a temperature above about 120 0 C (25O 0 F) until the moisture levels decreased to approximately 10% by weight. The pieces were then finish-dried at about HO 0 C ( 230 0 F) for about 10 minutes until the moisture content decreased to about 1.7-2.2% by weight. Final acrylamide levels of about 100-200 ppb were reported. However, when several samples of partially-baked pieces were finish-dried at about 120 0 C (25O 0 F) to about 1.6% moisture by weight, acrylamide levels of between 470 and 750 ppb were reported.
- substantially higher acrylamide levels of between 460 and 1900 ppb were produced when samples of partially- baked slices were finish-fried at about 132°C (27O 0 F) to about 1.6-2.2% moisture by weight. These results reeraphasize the importance of keeping the cooking or drying temperature of a cooking product at or below about 12O 0 C (250 0 F) during the final stages of cooking.
- This principle applies not only to the cooking of fabricated potato pieces but also to other fabricated products derived from potato, corn, barley, wheat, rye, rice, oats, millet, and other starch-based grains. This principle also applies to the cooking of raw foods such as yams and plantains.
- the modified cooking unit operation instead comprises vacuum frying for the entire cooking process.
- Figure 12 charts the results and operating conditions of several examples of such an embodiment.
- Tests 1-4 121 , 122, 123, 124 various control groups of peeled, sliced, 1.45 mm thick Hermes chipping potatoes were washed in ambient-temperature water for about 30 seconds, then processed through a standard continuous flyer.
- the fryer inlet oil temperature was varied within the range of about 165 to about 18O 0 C (329-356°), and the
- Tests 6 and 7 126, 127 show that vacuum frying under about 120 0 C (250 0 F) and at a pressure of no greater than 100 millibars results in virtually undetectable levels (less than 5 ppb) of acrylamide.
- the advantage of frying under about 120 0 C (25O 0 F) is that little to no acrylamide is formed, whereas high-temperature par- frying causes at least some acrylamide to form.
- temperatures greater than about 12O 0 C (25O 0 F) can be used when vacuum frying or vacuum finish-frying is employed, while still achieving a reduced acrylamide concentration in the finished product.
- the modified cooking unit operation can alternatively comprise low-temperature baking for the entire cooking process. Low-temperature baking can be performed at or below about 120 0 C (25O 0 F) so that little to no acrylamide is
- This invention contemplates combining the teachings herein with regard to various unit operation manipulations in order to achieve a desired acrylamide level in the end product along with the desired end-product characteristics.
- the combinations used depend on the starting product and the desired end product and can be adjusted by one skilled in the art pursuant to the teachings herein.
- the effect of pH on acrylamide formation is another factor that may be considered and combined with the teachings herein.
- changes in the characteristics of the final product such as changes in color, taste, and consistency can be adjusted by various means. For example, color characteristics in potato chips can be adjusted by controlling the amount of sugars in the starting product. Some flavor characteristics can be changed by the addition of various flavoring agents to the end product.
- the physical texture of the product can be adjusted by, for example, the addition of leavening agents or various emulsifiers.
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Priority Applications (9)
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CA2676330A CA2676330C (en) | 2007-01-26 | 2008-01-21 | Method for reducing acrylamide formation in thermally processed foods |
KR1020097017733A KR101302319B1 (en) | 2007-01-26 | 2008-01-21 | Reducing acrylamide formation in thermally processed foods |
EP08727990.7A EP2124622B1 (en) | 2007-01-26 | 2008-01-21 | Method for reducing acrylamide formation in thermally processed foods |
BRPI0806384-2A BRPI0806384A2 (en) | 2007-01-26 | 2008-01-21 | method for reducing asparagine content in a food product or ingredient and method for reducing acrylamide formation in thermally processed food products |
JP2009547369A JP5242592B2 (en) | 2007-01-26 | 2008-01-21 | Method for reducing acrylamide formation in heat processed foods |
ES08727990.7T ES2666655T3 (en) | 2007-01-26 | 2008-01-21 | Procedure to reduce acrylamide formation in heat treated foods |
AU2008208045A AU2008208045B2 (en) | 2007-01-26 | 2008-01-21 | Reducing acrylamide formation in thermally processed foods |
EG2009071113A EG25863A (en) | 2007-01-26 | 2009-07-21 | Method for reducing acrylamide formation in thermally processed foods |
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Also Published As
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RU2423875C2 (en) | 2011-07-20 |
KR20090117751A (en) | 2009-11-12 |
CN101641021A (en) | 2010-02-03 |
ES2666655T3 (en) | 2018-05-07 |
WO2008091821A3 (en) | 2008-10-16 |
MX2009007950A (en) | 2009-09-10 |
CA2676330C (en) | 2013-08-20 |
AU2008208045A1 (en) | 2008-07-31 |
EP2124622A4 (en) | 2015-05-27 |
CA2676330A1 (en) | 2008-07-31 |
EP2124622B1 (en) | 2018-03-07 |
EG25863A (en) | 2012-09-12 |
AU2008208045B2 (en) | 2012-07-12 |
CL2008000215A1 (en) | 2008-09-26 |
BRPI0806384A2 (en) | 2011-09-06 |
EP2124622A2 (en) | 2009-12-02 |
US20070141227A1 (en) | 2007-06-21 |
ZA200904984B (en) | 2010-08-25 |
RU2009131997A (en) | 2011-03-10 |
KR101302319B1 (en) | 2013-08-30 |
JP5242592B2 (en) | 2013-07-24 |
AR065047A1 (en) | 2009-05-13 |
JP2010516283A (en) | 2010-05-20 |
US7811618B2 (en) | 2010-10-12 |
TW200840488A (en) | 2008-10-16 |
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