WO2008084869A1 - 一分子型生物発光可視化プローブ - Google Patents

一分子型生物発光可視化プローブ Download PDF

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Publication number
WO2008084869A1
WO2008084869A1 PCT/JP2008/050370 JP2008050370W WO2008084869A1 WO 2008084869 A1 WO2008084869 A1 WO 2008084869A1 JP 2008050370 W JP2008050370 W JP 2008050370W WO 2008084869 A1 WO2008084869 A1 WO 2008084869A1
Authority
WO
WIPO (PCT)
Prior art keywords
ligand
molecule
lbd
domain
binding molecule
Prior art date
Application number
PCT/JP2008/050370
Other languages
English (en)
French (fr)
Inventor
Yoshio Umezawa
Moritoshi Sato
Hiroaki Tao
Sungbae Kim
Original Assignee
The University Of Tokyo
National Institute Of Advanced Industrial Science And Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The University Of Tokyo, National Institute Of Advanced Industrial Science And Technology filed Critical The University Of Tokyo
Priority to JP2008553132A priority Critical patent/JP5099560B2/ja
Priority to US12/448,881 priority patent/US8124424B2/en
Publication of WO2008084869A1 publication Critical patent/WO2008084869A1/ja

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Abstract

　 【課題】　シグナルの有無を指標として標的特異的リガンドを簡便かつ正確に検出することのできる単一分子型のプローブを提供する。 【解決手段】　生細胞における標的特異的リガンドを検出する一分子型生物発光可視化プローブであり、リガンドを結合した場合にその立体構造を変化させるリガンド結合分子の両端に、発光酵素（LE）を分割したN末端側ポリペプチド（N-LE）とC末端側ポリペプチド（C-LE）とがそれぞれ連結近接され、リガンド結合分子にリガンドが結合した場合のみ、N-LEとC-LEとが可逆的に自己相補して発光シグナルを発する。リガンド結合分子は、例えば、リガンド結合ドメイン（LBD）と、リガンドの結合によってLBDと相互作用するドメイン（LBD相互作用ドメイン）あるいはペプチドとが連結した融合分子である。
PCT/JP2008/050370 2007-01-12 2008-01-15 一分子型生物発光可視化プローブ WO2008084869A1 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2008553132A JP5099560B2 (ja) 2007-01-12 2008-01-15 一分子型生物発光可視化プローブ
US12/448,881 US8124424B2 (en) 2007-01-12 2008-01-15 Single molecule-format bioluminescent probe

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007005144 2007-01-12
JP2007-005144 2007-01-21

Publications (1)

Publication Number Publication Date
WO2008084869A1 true WO2008084869A1 (ja) 2008-07-17

Family

ID=39608756

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/050370 WO2008084869A1 (ja) 2007-01-12 2008-01-15 一分子型生物発光可視化プローブ

Country Status (3)

Country Link
US (1) US8124424B2 (ja)
JP (1) JP5099560B2 (ja)
WO (1) WO2008084869A1 (ja)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010119721A1 (ja) 2009-04-17 2010-10-21 独立行政法人産業技術総合研究所 超高輝度で安定な人工生物発光酵素
WO2014065047A1 (ja) 2012-10-26 2014-05-01 独立行政法人産業技術総合研究所 人工生物発光酵素
WO2016127302A1 (zh) * 2015-02-10 2016-08-18 深圳市第二人民医院 检测细胞外基质mmp13的bret探针、基因、表达载体和构建方法
JP2016163559A (ja) * 2015-02-26 2016-09-08 富山県 Ｖｄｒビタミンｄ結合ドメインを含む融合タンパク質及びその使用
US10214766B2 (en) 2013-10-18 2019-02-26 National Institute Of Advanced Industrial Science And Technology Luminescent substrate for use in artificial bioluminescent enzyme

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009153399A (ja) * 2007-12-25 2009-07-16 National Institute Of Advanced Industrial & Technology 一分子型リアルタイム生物発光イメージングプローブ
WO2016139643A2 (en) * 2015-03-04 2016-09-09 University College Dublin, National University Of Ireland, Dublin Molecular sensors

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004104222A1 (ja) * 2003-05-22 2004-12-02 Japan Science And Technology Agency 蛋白質相互作用解析用プローブ及びそれを用いた蛋白質相互作用の解析方法
WO2005078119A1 (ja) * 2004-02-12 2005-08-25 Japan Science And Technology Agency 核内レセプターのアゴニスト・アンタゴニスト検出用プローブとそれを用いた核内レセプターに対するアゴニストおよびアンタゴニストのスクリーニング方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7834148B2 (en) * 2006-05-23 2010-11-16 Stanford University Protein phosphorylation imaging systems, methods of making phosphorylation imaging systems, and methods of use thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004104222A1 (ja) * 2003-05-22 2004-12-02 Japan Science And Technology Agency 蛋白質相互作用解析用プローブ及びそれを用いた蛋白質相互作用の解析方法
WO2005078119A1 (ja) * 2004-02-12 2005-08-25 Japan Science And Technology Agency 核内レセプターのアゴニスト・アンタゴニスト検出用プローブとそれを用いた核内レセプターに対するアゴニストおよびアンタゴニストのスクリーニング方法

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
AWAIS M. ET AL.: "A fluorescent indicator to visualize activities of the androgen receptor ligands in single living cells", ANGEW. CHEM. INT. ED. ENGL., vol. 45, no. 17, 2006, pages 2707 - 2712 *
KIM S.B. ET AL.: "A method for determining the activities of cytokines based on the nuclear transport of nuclear factor-kappaB", ANAL. BIOCHEM., vol. 359, no. 1, 2006, pages 147 - 149, XP024942282, DOI: doi:10.1016/j.ab.2006.07.013 *
KIM S.B. ET AL.: "Bioluminescent indicator for determining protein-protein interactions using intramolecular complementation of split click beetle luciferase", ANAL. CHEM., vol. 79, no. 13, 1 July 2007 (2007-07-01), pages 4820 - 4826 *
KIM S.B. ET AL.: "Integrated molecule-format bioluminescent probe for visualizing androgenicity of ligands based on the intramolecular association of androgen receptor with its recognition Peptide", ANAL. CHEM., vol. 79, no. 5, 1 March 2007 (2007-03-01), pages 1874 - 1880, XP008123064, DOI: doi:10.1021/AC061934U *
PAULMURUGAN R. ET AL.: "Firefly luciferase enzyme fragment complementation for imaging in cells and living animals", ANAL. CHEM., vol. 77, no. 5, 2005, pages 1295 - 1302, XP002506343, DOI: doi:10.1021/AC0484777 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010119721A1 (ja) 2009-04-17 2010-10-21 独立行政法人産業技術総合研究所 超高輝度で安定な人工生物発光酵素
WO2014065047A1 (ja) 2012-10-26 2014-05-01 独立行政法人産業技術総合研究所 人工生物発光酵素
US10533231B2 (en) 2012-10-26 2020-01-14 National Institute Of Advanced Industrial Science And Technology Artificial bioluminescent enzyme
US10214766B2 (en) 2013-10-18 2019-02-26 National Institute Of Advanced Industrial Science And Technology Luminescent substrate for use in artificial bioluminescent enzyme
WO2016127302A1 (zh) * 2015-02-10 2016-08-18 深圳市第二人民医院 检测细胞外基质mmp13的bret探针、基因、表达载体和构建方法
JP2016163559A (ja) * 2015-02-26 2016-09-08 富山県 Ｖｄｒビタミンｄ結合ドメインを含む融合タンパク質及びその使用

Also Published As

Publication number Publication date
JP5099560B2 (ja) 2012-12-19
US8124424B2 (en) 2012-02-28
JPWO2008084869A1 (ja) 2010-05-06
US20100273150A1 (en) 2010-10-28

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