WO2008067032A3 - Transgenic mice expressing a real-time reporter for cellular camp in a tissue-selective and inducible manner - Google Patents

Transgenic mice expressing a real-time reporter for cellular camp in a tissue-selective and inducible manner Download PDF

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Publication number
WO2008067032A3
WO2008067032A3 PCT/US2007/080299 US2007080299W WO2008067032A3 WO 2008067032 A3 WO2008067032 A3 WO 2008067032A3 US 2007080299 W US2007080299 W US 2007080299W WO 2008067032 A3 WO2008067032 A3 WO 2008067032A3
Authority
WO
WIPO (PCT)
Prior art keywords
tissue
selective
real
transgenic mice
mice expressing
Prior art date
Application number
PCT/US2007/080299
Other languages
French (fr)
Other versions
WO2008067032A2 (en
Inventor
Nirupa Chaudhari
Stephen D Roper
Original Assignee
Univ Miami
Nirupa Chaudhari
Stephen D Roper
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Miami, Nirupa Chaudhari, Stephen D Roper filed Critical Univ Miami
Publication of WO2008067032A2 publication Critical patent/WO2008067032A2/en
Publication of WO2008067032A3 publication Critical patent/WO2008067032A3/en

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/035—Animal model for multifactorial diseases
    • A01K2267/0362—Animal model for lipid/glucose metabolism, e.g. obesity, type-2 diabetes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C12N2830/003—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor tet inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination

Abstract

A transgenic mouse expressing a functioning protein reporter for cAMP expression. The c AMP is under control of a tissue specific and inducible promoter. The transgenic mouse allows for the continuous measurement of c AMP dynamics; identifying cell signaling events; identifying novel targets for drugs. Vectors and methods of use are further described.
PCT/US2007/080299 2006-10-03 2007-10-03 Transgenic mice expressing a real-time reporter for cellular camp in a tissue-selective and inducible manner WO2008067032A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US84909206P 2006-10-03 2006-10-03
US60/849,092 2006-10-03

Publications (2)

Publication Number Publication Date
WO2008067032A2 WO2008067032A2 (en) 2008-06-05
WO2008067032A3 true WO2008067032A3 (en) 2008-10-30

Family

ID=39468563

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/080299 WO2008067032A2 (en) 2006-10-03 2007-10-03 Transgenic mice expressing a real-time reporter for cellular camp in a tissue-selective and inducible manner

Country Status (1)

Country Link
WO (1) WO2008067032A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010049828A1 (en) * 2000-04-12 2001-12-06 Orosz Charles G. Genetic system for controlling background expression of transgene products

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010049828A1 (en) * 2000-04-12 2001-12-06 Orosz Charles G. Genetic system for controlling background expression of transgene products

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DYACHOK O. ET AL.: "Oscillations of cyclic AMP in hormone-stimulated insulin secreting beta cells", NATURE, vol. 439, 19 January 2006 (2006-01-19), pages 349 - 352 *
NIKOLAEV V.O. ET AL.: "Novel single chain cAMP sensors for receptor-induced signal propagation", J. BIOL. CHEM., vol. 279, no. 36, September 2004 (2004-09-01), pages 37215 - 37218 *

Also Published As

Publication number Publication date
WO2008067032A2 (en) 2008-06-05

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