WO2007038757A3 - Methods and compositions for non-invasive assessment of gene expression - Google Patents

Methods and compositions for non-invasive assessment of gene expression Download PDF

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Publication number
WO2007038757A3
WO2007038757A3 PCT/US2006/038176 US2006038176W WO2007038757A3 WO 2007038757 A3 WO2007038757 A3 WO 2007038757A3 US 2006038176 W US2006038176 W US 2006038176W WO 2007038757 A3 WO2007038757 A3 WO 2007038757A3
Authority
WO
WIPO (PCT)
Prior art keywords
reporter rna
encapsidated
rna
compositions
methods
Prior art date
Application number
PCT/US2006/038176
Other languages
French (fr)
Other versions
WO2007038757A2 (en
Inventor
Sergei Romanov
Ming Zeng
Sergei Makarov
Original Assignee
Attagene Inc
Sergei Romanov
Ming Zeng
Sergei Makarov
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Attagene Inc, Sergei Romanov, Ming Zeng, Sergei Makarov filed Critical Attagene Inc
Publication of WO2007038757A2 publication Critical patent/WO2007038757A2/en
Publication of WO2007038757A3 publication Critical patent/WO2007038757A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Abstract

The present invention provides methods and compositions relating to assays for detection of RNA transcription, transcription factor activity, apoptosis and other cellular activities. For example, in some aspects the present invention provides methods of extracellularly detecting a reporter RNA wherein the reporter RNA is extracellular released in an encapsidated particle from a target cell comprising a packaging polypeptide, wherein the encapsidated particle comprises the packaging polypeptide, and wherein the reporter RNA is intracellularly transcribed in the target cell. In other aspects, the present invention provides a host cell that extracellularly releases a reporter RNA in an encapsidated particle comprising a packaging protein, wherein the reporter RNA is intracellularly transcribed.
PCT/US2006/038176 2005-09-28 2006-09-27 Methods and compositions for non-invasive assessment of gene expression WO2007038757A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US72184405P 2005-09-28 2005-09-28
US60/721,844 2005-09-28

Publications (2)

Publication Number Publication Date
WO2007038757A2 WO2007038757A2 (en) 2007-04-05
WO2007038757A3 true WO2007038757A3 (en) 2007-11-29

Family

ID=37900496

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/038176 WO2007038757A2 (en) 2005-09-28 2006-09-27 Methods and compositions for non-invasive assessment of gene expression

Country Status (1)

Country Link
WO (1) WO2007038757A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103930566B (en) * 2011-09-08 2018-01-26 埃塔格尼公司 System and method for determining organism in water pollutant
US10544472B2 (en) 2014-01-18 2020-01-28 Attagene, Inc. Multiplexing transcription factor reporter protein assay process and system
FR3036118B1 (en) * 2015-05-15 2019-07-12 Flash Therapeutics RETROVIRAL PARTICLE COMPRISING AT LEAST TWO ENCAPSID NON-VIRAL RNA
SG11201709336SA (en) 2015-05-15 2017-12-28 Vectalys Retroviral particle comprising at least two encapsidated non-viral rnas
CN112921034B (en) * 2019-12-05 2023-11-21 重庆精准生物技术有限公司 Promoter for regulating and controlling CAR-T specific activation and application thereof
CN112921035B (en) * 2019-12-05 2023-11-14 重庆精准生物技术有限公司 Promoter for regulating and controlling CAR-T specific activation and application thereof

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6015686A (en) * 1993-09-15 2000-01-18 Chiron Viagene, Inc. Eukaryotic layered vector initiation systems
US6203986B1 (en) * 1998-10-22 2001-03-20 Robert H. Singer Visualization of RNA in living cells
US20020009747A1 (en) * 1997-08-25 2002-01-24 Freda Diane Miller Methods and reagents for identifying modulators of neuronal apoptosis
US20030104422A1 (en) * 2001-05-25 2003-06-05 Vincent Thuillier Engineered secreted alkaline phosphatase (SEAP) reporter genes and polypeptides
US20030143540A1 (en) * 2000-12-28 2003-07-31 Asahi Kasei Kabushiki Kaisha NF-kB Activating Gene
US20040005564A1 (en) * 2000-01-28 2004-01-08 Mauro Vincent P. Methods of identifying synthetic transcriptonal and translational regulatory elements, and compositions relating to same
US20040209274A2 (en) * 2001-03-09 2004-10-21 Gene Stream Pty Ltd Constructs for Gene Expression Analysis

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6015686A (en) * 1993-09-15 2000-01-18 Chiron Viagene, Inc. Eukaryotic layered vector initiation systems
US20020009747A1 (en) * 1997-08-25 2002-01-24 Freda Diane Miller Methods and reagents for identifying modulators of neuronal apoptosis
US6203986B1 (en) * 1998-10-22 2001-03-20 Robert H. Singer Visualization of RNA in living cells
US20040005564A1 (en) * 2000-01-28 2004-01-08 Mauro Vincent P. Methods of identifying synthetic transcriptonal and translational regulatory elements, and compositions relating to same
US20030143540A1 (en) * 2000-12-28 2003-07-31 Asahi Kasei Kabushiki Kaisha NF-kB Activating Gene
US20040209274A2 (en) * 2001-03-09 2004-10-21 Gene Stream Pty Ltd Constructs for Gene Expression Analysis
US20030104422A1 (en) * 2001-05-25 2003-06-05 Vincent Thuillier Engineered secreted alkaline phosphatase (SEAP) reporter genes and polypeptides

Also Published As

Publication number Publication date
WO2007038757A2 (en) 2007-04-05

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