WO2008034896A3 - Identification et quantification d'une pluralité de (micro)organismes biologiques ou de leurs composants - Google Patents

Identification et quantification d'une pluralité de (micro)organismes biologiques ou de leurs composants Download PDF

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Publication number
WO2008034896A3
WO2008034896A3 PCT/EP2007/060037 EP2007060037W WO2008034896A3 WO 2008034896 A3 WO2008034896 A3 WO 2008034896A3 EP 2007060037 W EP2007060037 W EP 2007060037W WO 2008034896 A3 WO2008034896 A3 WO 2008034896A3
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WO
WIPO (PCT)
Prior art keywords
solid support
amplicons
pcr
quantification
detection
Prior art date
Application number
PCT/EP2007/060037
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English (en)
Other versions
WO2008034896A8 (fr
WO2008034896A2 (fr
Inventor
Isabelle Alexandre
Dieter Husar
Nathalie Zammatteo
Heinz Koehn
Jose Remacle
Sylvain Margaine
Original Assignee
Eppendorf Array Tech Sa
Isabelle Alexandre
Dieter Husar
Nathalie Zammatteo
Heinz Koehn
Jose Remacle
Sylvain Margaine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf Array Tech Sa, Isabelle Alexandre, Dieter Husar, Nathalie Zammatteo, Heinz Koehn, Jose Remacle, Sylvain Margaine filed Critical Eppendorf Array Tech Sa
Priority to EP07803579A priority Critical patent/EP2069069A2/fr
Publication of WO2008034896A2 publication Critical patent/WO2008034896A2/fr
Publication of WO2008034896A3 publication Critical patent/WO2008034896A3/fr
Publication of WO2008034896A8 publication Critical patent/WO2008034896A8/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)

Abstract

L'invention concerne un système et un procédé de conduite d'une amplification en chaîne par polymérase (PCR) en temps réel. Des molécules de capture d'une conception particulière sont immobilisées sur un support solide, et mises en contact avec des amplicons obtenus en un ou plusieurs cycles de PCR. La détection des amplicons peut avoir lieu pendant ou entre les cycles de PCR, tandis que le support solide est en contact fluidique avec la solution de PCR. Dans un mode de réalisation, la détection des amplicons a lieu lorsque le support solide n'est pas en contact fluidique avec la solution de PCR. Dans un autre mode de réalisation, des amplicons marqués sont détectés à l'aide d'un champ évanescent tout en étant hybridés pour capturer des sondes immobilisées sur un support solide dans la chambre de réaction de PCR en temps réel. Le procédé est approprié pour la détection et la quantification simultanées de molécules cibles étroitement homologues.
PCT/EP2007/060037 2006-09-22 2007-09-21 Identification et quantification d'une pluralité de (micro)organismes biologiques ou de leurs composants WO2008034896A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP07803579A EP2069069A2 (fr) 2006-09-22 2007-09-21 Identification et quantification d'une pluralité de (micro)organismes biologiques ou de leurs composants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52615906A 2006-09-22 2006-09-22
US11/526,159 2006-09-22

Publications (3)

Publication Number Publication Date
WO2008034896A2 WO2008034896A2 (fr) 2008-03-27
WO2008034896A3 true WO2008034896A3 (fr) 2008-05-08
WO2008034896A8 WO2008034896A8 (fr) 2009-11-05

Family

ID=38828646

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/060037 WO2008034896A2 (fr) 2006-09-22 2007-09-21 Identification et quantification d'une pluralité de (micro)organismes biologiques ou de leurs composants

Country Status (2)

Country Link
EP (1) EP2069069A2 (fr)
WO (1) WO2008034896A2 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2107125A1 (fr) * 2008-03-31 2009-10-07 Eppendorf Array Technologies SA (EAT) PCR en temps réel de cibles sur un micro-réseau
DE102009044431A1 (de) 2009-11-05 2011-06-22 FRIZ Biochem Gesellschaft für Bioanalytik mbH, 82061 Vorrichtung zur Durchführung einer PCR
EP2441520A1 (fr) 2010-10-12 2012-04-18 Eppendorf AG Application en temps réel et détection basée sur un micro-réseau de cibles d'acides nucléiques dans un dosage sur puce de flux
CN103347995B (zh) * 2011-01-31 2015-07-08 株式会社日立高新技术 核酸检查装置
KR101168165B1 (ko) * 2012-02-29 2012-07-24 케이맥(주) 바이오 리액션 디바이스 칩
KR101184566B1 (ko) 2012-05-11 2012-09-20 케이맥(주) 실시간 중합효소 연쇄반응과 ｄｎａ 칩이 통합된 검사 시스템 및 이를 이용한 통합 분석방법
US11215613B2 (en) * 2013-10-31 2022-01-04 Konica Minolta, Inc. Detection device, detection method using said detection device, and detection chip used in said detection device
KR101707456B1 (ko) * 2015-10-01 2017-02-17 한국과학기술원 필름 기반 유전자 증폭 장치
CN108884430B (zh) 2016-04-20 2019-11-19 希森美康株式会社 核酸分析装置及核酸分析方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6620623B1 (en) * 2002-05-06 2003-09-16 The University Of Chicago Biochip reader with enhanced illumination and bioarray positioning apparatus
US20060088844A1 (en) * 2004-10-22 2006-04-27 Honeywell International Inc. Real-time PCR microarray based on evanescent wave biosensor

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6620623B1 (en) * 2002-05-06 2003-09-16 The University Of Chicago Biochip reader with enhanced illumination and bioarray positioning apparatus
US20060088844A1 (en) * 2004-10-22 2006-04-27 Honeywell International Inc. Real-time PCR microarray based on evanescent wave biosensor

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
HEALEY B G ET AL: "FIBEROPTIC DNA SENSOR ARRAY CAPABLE OF DETECTING POINT MUTATIONS", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 251, no. 2, 5 September 1997 (1997-09-05), pages 270 - 279, XP000703841, ISSN: 0003-2697 *
LEHR H-P ET AL: "REAL-TIME DETECTION OF NUCLEIC ACID INTERACTIONS BY TOTAL INTERNAL REFLECTION FLUORESCENCE", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, vol. 75, no. 10, 15 May 2003 (2003-05-15), pages 2414 - 2420, XP001170913, ISSN: 0003-2700 *
ROBIN HUI LIU ET AL: "SELF-CONTAINED, FULLY INTEGRATED BIOCHIP FOR SAMPLE PREPARATION, POLYMERASE CHAIN REACTION AMPLIFICATION, AND DNA MICROARRAY DETECTION", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, vol. 76, no. 7, 1 April 2004 (2004-04-01), pages 1824 - 1831, XP001196720, ISSN: 0003-2700 *
SCHENA MARK: "Microarray Analysis", 2003, JOHN WILEY & SONS, NEW JERSEY, XP002463284 *
STIMPSON D I ET AL: "REAL-TIME DETECTION OF DNA HYBRIDIZATION AND MELTING ON OLIGONUCLEOTIDE ARRAYS BY USING OPTICAL WAVE GUIDES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 92, July 1995 (1995-07-01), pages 6379 - 6383, XP002912130, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
WO2008034896A8 (fr) 2009-11-05
EP2069069A2 (fr) 2009-06-17
WO2008034896A2 (fr) 2008-03-27

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