WO2008030858A3 - Use of esterase expressed in plants for the control of gram-negative bacteria - Google Patents

Use of esterase expressed in plants for the control of gram-negative bacteria Download PDF

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Publication number
WO2008030858A3
WO2008030858A3 PCT/US2007/077614 US2007077614W WO2008030858A3 WO 2008030858 A3 WO2008030858 A3 WO 2008030858A3 US 2007077614 W US2007077614 W US 2007077614W WO 2008030858 A3 WO2008030858 A3 WO 2008030858A3
Authority
WO
WIPO (PCT)
Prior art keywords
negative bacteria
esterase
gram
control
present
Prior art date
Application number
PCT/US2007/077614
Other languages
French (fr)
Other versions
WO2008030858A2 (en
Inventor
Dean W Gabriel
Joseph D Reddy
Original Assignee
Integrated Plant Genetics Inc
Dean W Gabriel
Joseph D Reddy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Integrated Plant Genetics Inc, Dean W Gabriel, Joseph D Reddy filed Critical Integrated Plant Genetics Inc
Publication of WO2008030858A2 publication Critical patent/WO2008030858A2/en
Publication of WO2008030858A3 publication Critical patent/WO2008030858A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8266—Abscission; Dehiscence; Senescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

Abstract

The present invention provides various methods of using esterase to control gram-negative bacteria. The present invention also provides transgenic plants expressing esterase and methods of their production and use. The present invention finds applications in many fields, including plant husbandry, water purification, food safety, bioterrorism prevention, and cut flower maintenance.
PCT/US2007/077614 2006-09-05 2007-09-05 Use of esterase expressed in plants for the control of gram-negative bacteria WO2008030858A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82448006P 2006-09-05 2006-09-05
US60/824,480 2006-09-05

Publications (2)

Publication Number Publication Date
WO2008030858A2 WO2008030858A2 (en) 2008-03-13
WO2008030858A3 true WO2008030858A3 (en) 2008-07-24

Family

ID=39157995

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/077614 WO2008030858A2 (en) 2006-09-05 2007-09-05 Use of esterase expressed in plants for the control of gram-negative bacteria

Country Status (1)

Country Link
WO (1) WO2008030858A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102703436A (en) * 2012-06-15 2012-10-03 赣南师范学院 Method for extracting DNA of citrus canker by water bath precipitation
CN103070065B (en) * 2013-01-18 2014-10-15 湖北省种子集团有限公司 Amphibious movable seed production device and operating method thereof
JP6997456B2 (en) * 2016-05-25 2022-01-17 国立研究開発法人農業・食品産業技術総合研究機構 Compositions for enhancing plant disease resistance or for controlling plant diseases and their usage methods
CN108060150B (en) * 2017-12-01 2021-01-26 华南农业大学 Protease PASE4 with nematicidal activity and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6063610A (en) * 1996-11-12 2000-05-16 Heska Corporation Carboxylesterase nucleic acid molecules, proteins and uses thereof
US6375947B1 (en) * 1998-11-05 2002-04-23 International Flavors & Fragrances Inc. Recombinant kid pregastric esterase and methods for its production and use
US7214860B2 (en) * 2004-02-20 2007-05-08 Pioneer Hi-Bred International, Inc. Methods of using non-plant encoding nucleic acids

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6063610A (en) * 1996-11-12 2000-05-16 Heska Corporation Carboxylesterase nucleic acid molecules, proteins and uses thereof
US6375947B1 (en) * 1998-11-05 2002-04-23 International Flavors & Fragrances Inc. Recombinant kid pregastric esterase and methods for its production and use
US7214860B2 (en) * 2004-02-20 2007-05-08 Pioneer Hi-Bred International, Inc. Methods of using non-plant encoding nucleic acids

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ZHANG ET AL.: "The Gene for Albicidin Detoxification from Pantoea Dispersa Encodes an Esterase and Attenuates Pathogenicity of Xanthomonas albilineans to Sugarcane", PNAS, vol. 94, September 1997 (1997-09-01), pages 9984 - 9989, XP002364394, DOI: doi:10.1073/pnas.94.18.9984 *

Also Published As

Publication number Publication date
WO2008030858A2 (en) 2008-03-13

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