WO2008022718A8 - Method for detecting electrically conductive macromolecules - Google Patents

Method for detecting electrically conductive macromolecules

Info

Publication number
WO2008022718A8
WO2008022718A8 PCT/EP2007/007079 EP2007007079W WO2008022718A8 WO 2008022718 A8 WO2008022718 A8 WO 2008022718A8 EP 2007007079 W EP2007007079 W EP 2007007079W WO 2008022718 A8 WO2008022718 A8 WO 2008022718A8
Authority
WO
WIPO (PCT)
Prior art keywords
macromolecules
macromolecule
magnetic field
electrically conductive
magnetic
Prior art date
Application number
PCT/EP2007/007079
Other languages
German (de)
French (fr)
Other versions
WO2008022718A1 (en
Inventor
Holger Muehlberger
Werner Hoffmann
Original Assignee
Karlsruhe Forschzent
Holger Muehlberger
Werner Hoffmann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Karlsruhe Forschzent, Holger Muehlberger, Werner Hoffmann filed Critical Karlsruhe Forschzent
Priority to EP07801589A priority Critical patent/EP2054717A2/en
Publication of WO2008022718A1 publication Critical patent/WO2008022718A1/en
Publication of WO2008022718A8 publication Critical patent/WO2008022718A8/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/4473—Arrangements for investigating the separated zones, e.g. localising zones by electric means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y25/00—Nanomagnetism, e.g. magnetoimpedance, anisotropic magnetoresistance, giant magnetoresistance or tunneling magnetoresistance
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus

Abstract

The invention relates to a method for detecting individual or a few electrically conductive macromolecules, preferably nucleic acids or proteins, which are present in an ion-conducting fluid such that they move freely. It is an object of the invention to provide a method for detecting the macromolecules, without the need to provide the macromelecules in advance with a magnetic marker or label. This method is also intended to enable the manipulation of the macromolecules, particularly for transporting them for analysis, for separating them or for enriching them. This object is achieved by the following method. First, an ion-conducting fluid, in which the macromolecule (4) which is free from magnetic markers is located, is added to a volume (1). Subsequently, an electric field is applied over the volume, which produces a first magnetic field around the at least one electroconductive macromolecule. Finally, a magnetic field sensor (5) is used to ascertain the magnetic field or its temporal change, by means of which the macromolecule can be detected.
PCT/EP2007/007079 2006-08-22 2007-08-10 Method for detecting electrically conductive macromolecules WO2008022718A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP07801589A EP2054717A2 (en) 2006-08-22 2007-08-10 Method for detecting electrically conductive macromolecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102006039370.8 2006-08-22
DE200610039370 DE102006039370A1 (en) 2006-08-22 2006-08-22 Method for analyzing macromolecules and device for carrying out the method

Publications (2)

Publication Number Publication Date
WO2008022718A1 WO2008022718A1 (en) 2008-02-28
WO2008022718A8 true WO2008022718A8 (en) 2008-09-25

Family

ID=38698732

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/007079 WO2008022718A1 (en) 2006-08-22 2007-08-10 Method for detecting electrically conductive macromolecules

Country Status (3)

Country Link
EP (1) EP2054717A2 (en)
DE (1) DE102006039370A1 (en)
WO (1) WO2008022718A1 (en)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6920000A (en) * 1999-08-21 2001-03-19 John S. Fox High sensitivity biomolecule detection with magnetic particles
US6992482B2 (en) * 2000-11-08 2006-01-31 Jentek Sensors, Inc. Magnetic field sensor having a switchable drive current spatial distribution
US6736978B1 (en) * 2000-12-13 2004-05-18 Iowa State University Research Foundation, Inc. Method and apparatus for magnetoresistive monitoring of analytes in flow streams
US7560927B2 (en) * 2003-08-28 2009-07-14 Massachusetts Institute Of Technology Slitted and stubbed microstrips for high sensitivity, near-field electromagnetic detection of small samples and fields

Also Published As

Publication number Publication date
EP2054717A2 (en) 2009-05-06
DE102006039370A1 (en) 2008-02-28
WO2008022718A1 (en) 2008-02-28

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