WO2008019941A1 - PROCÉDÉ POUR SÉPARER ET CONCENTRER DE la BIOMASSE - Google Patents

PROCÉDÉ POUR SÉPARER ET CONCENTRER DE la BIOMASSE Download PDF

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Publication number
WO2008019941A1
WO2008019941A1 PCT/EP2007/057877 EP2007057877W WO2008019941A1 WO 2008019941 A1 WO2008019941 A1 WO 2008019941A1 EP 2007057877 W EP2007057877 W EP 2007057877W WO 2008019941 A1 WO2008019941 A1 WO 2008019941A1
Authority
WO
WIPO (PCT)
Prior art keywords
range
membrane
biomass
concentration
carries out
Prior art date
Application number
PCT/EP2007/057877
Other languages
German (de)
English (en)
Inventor
Lars Kucka
Wilfried Rähse
Werner Pichler
Original Assignee
Henkel Ag & Co. Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Henkel Ag & Co. Kgaa filed Critical Henkel Ag & Co. Kgaa
Publication of WO2008019941A1 publication Critical patent/WO2008019941A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D33/00—Filters with filtering elements which move during the filtering operation
    • B01D33/15—Filters with filtering elements which move during the filtering operation with rotary plane filtering surfaces
    • B01D33/21—Filters with filtering elements which move during the filtering operation with rotary plane filtering surfaces with hollow filtering discs transversely mounted on a hollow rotary shaft
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D33/00—Filters with filtering elements which move during the filtering operation
    • B01D33/35—Filters with filtering elements which move during the filtering operation with multiple filtering elements characterised by their mutual disposition
    • B01D33/37—Filters with filtering elements which move during the filtering operation with multiple filtering elements characterised by their mutual disposition in parallel connection
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D33/00—Filters with filtering elements which move during the filtering operation
    • B01D33/58—Handling the filter cake in the filter for purposes other than for regenerating the filter cake remaining on the filtering element
    • B01D33/68—Retarding cake deposition on the filter during the filtration period, e.g. using stirrers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
    • B01D63/16—Rotary, reciprocated or vibrated modules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2311/00—Details relating to membrane separation process operations and control
    • B01D2311/10—Temperature control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2315/00—Details relating to the membrane module operation
    • B01D2315/02—Rotation or turning
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2315/00—Details relating to the membrane module operation
    • B01D2315/14—Batch-systems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2315/00—Details relating to the membrane module operation
    • B01D2315/16—Diafiltration

Definitions

  • the present invention relates to a process for the separation and concentration of biomass from a fermentation broth by means of dynamic cross-flow filtration with rotating ceramic membranes.
  • DE 10022258 A1 describes a method for purifying and / or concentrating protein inclusion bodies in a solution, which comprises passing the solution containing the protein inclusion body tangentially past one or more semipermeable membranes, so that the protein inclusion bodies are retained by the membranes and substances or pass through components having a smaller molecular weight or particle diameter, whereby a purified and / or concentrated protein inclusion body solution is obtained.
  • a disadvantage of these known methods is also the problem of so-called fouling which always occurs in membrane processes. Due to the flow of material to the membrane surface, in addition to the recyclables, substances also reach the pores of the membrane, which due to their size can not pass through them. As a result, a complex composite covering layer is formed on the membrane surface, which more and more blocks the flow of material through the membrane, in particular of the valuable substance, during the concentration.
  • the retention (retention of valuable substances) is increased and the permeate flow is limited.
  • the concentration In order to maintain the separation efficiency of the process, the concentration must be stopped at a relatively low degree of concentration and the membrane surfaces must be subjected to a purification.
  • the object of the invention is therefore to provide a method for separating and concentrating biomass from a fermentation broth, which enables a low retention of the valuable material at high permeate flow and can be used universally. Furthermore, the method should be energy efficient and economically feasible.
  • This object is achieved according to the invention by a process for the separation and concentration of biomass from a fermentation broth by means of dynamic cross-flow filtration (cross-flow filtration), which is characterized in that rotating ceramic filtration membranes are used.
  • the process according to the invention is particularly suitable for the concentration of proteins, preferably of enzymes, in particular of detergent enzymes.
  • a first concentration step can be carried out in an upstream centrifuge stage.
  • the concentrate obtained from the centrifuging stage is preferably diafiltered with the addition of water and then further concentrated.
  • such an amount of water is added that the broth is diluted 1.5 to 4 times, more preferably 2 to 3 times.
  • This addition of water to the retentate increases the yield and hence the efficiency of the filtration process. Quantities of the desired product in the retentate may still be recovered by this dilution step involving the addition of water to the retentate.
  • the membranes are designed such that the biomass is retained on the surface of the retentate and the solvent is obtained with the enzyme to be recovered as valuable material and optionally together with low molecular weight compounds on the permeate side.
  • the permeate can then be further purified, for example subjected to ultrafiltration, directly for the final concentration of the enzyme in a manner known to the person skilled in the art.
  • the rotating ceramic filtration membranes are used so that they partially overlap.
  • a ceramic membrane is used which has an average pore size in the range of 0.01 to 1 .mu.m, in particular from 0.1 to 0.2 microns.
  • VCF volumetric Einkonzentri für styrene
  • This concentration may be at vorteilhaftesten at elevated temperatures, preferably at a temperature in the range of 1 ° C to 70 0 C, more preferably from 35 ° C to 70 0 C are performed.
  • the process is started at a temperature of about ⁇ 35 ° C and terminated at a temperature of about 70 0 C.
  • the process is preferably carried out such that more than 60% of the enzyme permeates and the retentate has a solids content of more than 12%.
  • the Transmembran horrin is in carrying out the method according to the invention in the range of 0.2 to 10 bar, preferably in the range of 0.7 to 3.0 bar.
  • shear rates in the range from 0.5 to 10 m / s, preferably in the range from 4 to 7 m / s, are achieved.
  • the process according to the invention can be carried out continuously. Preferably, however, it is carried out as a batch process, that is, a large volume to be purified is introduced once and then worked up. A continuous system is less advantageous in enzyme recovery, because the fermenters are typically also prepared batchwise and harvested.
  • Membrane materials suitable according to the invention are in principle all from which ceramic membranes can be produced.
  • the material of the membrane layer is preferably Ci-Al 2 O 3 , zirconium dioxide or titanium dioxide into consideration.
  • Rotary ceramic filtration membranes which can be used according to the invention are, for example, those which are also used in the devices described in DE 100 19 672 A1.
  • Devices of this type are used for the cross-flow permeation of flowable media. They comprise at least two shafts, on each of which many disk-shaped membrane bodies are arranged parallel to one another and at a mutual distance.
  • the waves are hollow, and the membrane discs are made of ceramic material and are crossed by radial channels. There is a conductive connection between the radial channels and the interior of the hollow shaft.
  • the liquid to be filtered passes through from the outside the porous material of the membrane body in the channels, and from there into the hollow shaft.
  • the said waves are parallel to each other, so that the membrane discs of two adjacent disk packages are arranged parallel to each other.
  • the waves are arranged so close together that the discs of two disc packs mesh with each other like a toothing.
  • the discs need not have the stated construction of porous ceramic material. There are also applications in which some discs are constructed as so-called dummy discs. It is also conceivable to produce the discs from sieve bodies. Combinations of the types mentioned are conceivable, for example, the pairing Sieb stresses-membrane body. The following is to be spoken only by "slices”.
  • spacers are arranged between two adjacent discs. Also in the sense of a precise axial positioning of the entire disc package is compressed, for example, by a nut which is screwed onto the respective hollow shaft on one end, and exerts a corresponding pressure on the plurality of discs with spacer elements located therebetween. Between mother and hollow shaft, a spring package can be switched.
  • Rotary ceramic filtration membranes which can preferably be used according to the invention are those which are also used in the devices described in WO 02005935 A2, to which reference is hereby made in their entirety.
  • FIG. 1 shows a device with filter plates in a schematic elevational view.
  • Figure 2 shows the article of Figure 1 in a plan view.
  • Figure 3 shows a modified embodiment of the article of Figure 1, again in plan view.
  • the device has two hollow shafts 1, 1 '.
  • Each hollow shaft carries a package of disks 2, 2 '.
  • the two shafts 1, 1 'and the disc packs are located in a container 10.
  • the container has an inlet 10.1 and an outlet 10.2.
  • the discs are used for filtration. They are constructed of a porous ceramic material and have channels in their interior. The channels are connected to the interiors of the hollow shafts 1, 1 'in a conductive connection.
  • the medium to be treated passes into the interior of the container 10.
  • the filtrate / permeate then passes through the pores of the ceramic material in said channels and from there into the interior of the two hollow shafts 1, 1 '. It then exits at the upper ends of the hollow shafts - see the two arrows pointing upwards.
  • the discs may be classic filter discs constructed of a porous ceramic material. But it may also be hollow screen body. Finally, at least some of the discs may be formed as so-called dummy discs.
  • the disks of the individual disk packs may be constructed of different filter media, which are different from each other in terms of their material or their deposition rates. This applies both within a chamber, and from one chamber to another.
  • rotary ceramic filtration membranes are those which are also used in the sold under the trade name RotoStream® by the company Canzler GmbH, Düren, or by the company. Andritz, Graz, devices
  • Membrane discs are mounted on rotating hollow shafts. The discs on the adjacent shafts overlap. The food product is guided along the outer surface of the discs. Permeate enters through the membrane into the interior of the discs, the support structure, and is discharged through the hollow shaft.
  • the support structure gives the windows the necessary mechanical stability. There is only a small flow resistance for the permeate when penetrating into the hollow shaft.
  • the permeation zone where the slices do not overlap
  • the self-cleaning zone where the topcoat is further broken apart beyond the high crossflow cycle cleaning effect.
  • the cover layer thickness is controlled by the rotational speed.
  • the present invention thus has a number of advantages over the prior art:
  • the cross flow cleaning effect and the TMP are independently adjustable.
  • Another object of the present invention is the use of a device according to the teaching of WO 02005935 A2 or DE 100 19 672 A1, or a device sold under the trade name RotoStream® by Canzler GmbH, Düren, or by the company. Andritz , Graz, for the separation and concentration of biomass from a fermentation broth by means of dynamic cross-flow filtration (cross-flow filtration).
  • the laboratory system comprises two shafts with the same direction of rotation and 0.136 m 2

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Water Supply & Treatment (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)

Abstract

La présente invention concerne un procédé pour séparer des enzymes et concentrer de la biomasse à partir d'un bouillon de fermentation au moyen d'une filtration dynamique à flux croisés avec des membranes tournantes se chevauchant (2, 2').
PCT/EP2007/057877 2006-08-15 2007-07-31 PROCÉDÉ POUR SÉPARER ET CONCENTRER DE la BIOMASSE WO2008019941A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102006038340A DE102006038340A1 (de) 2006-08-15 2006-08-15 Verfahren zur Abtrennung und Aufkonzentrierung von Biomasse
DE102006038340.0 2006-08-15

Publications (1)

Publication Number Publication Date
WO2008019941A1 true WO2008019941A1 (fr) 2008-02-21

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/057877 WO2008019941A1 (fr) 2006-08-15 2007-07-31 PROCÉDÉ POUR SÉPARER ET CONCENTRER DE la BIOMASSE

Country Status (2)

Country Link
DE (1) DE102006038340A1 (fr)
WO (1) WO2008019941A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111647025A (zh) * 2020-06-18 2020-09-11 甘肃天顺植物科技有限公司 一种从柳树中提取分离精制水杨苷的方法
US20210348201A1 (en) * 2020-05-06 2021-11-11 Alliance For Sustainable Energy, Llc Advanced anaerobic digestion to carboxylic acids

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102009010484A1 (de) * 2009-02-25 2010-08-26 Gea Westfalia Separator Gmbh Filtrationsverfahren und -vorrichtung
DE202013101302U1 (de) 2013-03-26 2013-05-06 Mst Microsieve Technologies Gmbh Filtrationsvorrichtung

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01296972A (ja) * 1988-05-25 1989-11-30 Ngk Insulators Ltd 膜型反応装置
US4911847A (en) * 1983-12-20 1990-03-27 Membrex, Inc. Process for controlling the permeate composition in a rotary filtration device
JPH08280391A (ja) * 1995-04-18 1996-10-29 Kao Corp 発酵生産物の回収方法
DE19617775A1 (de) * 1996-05-03 1997-11-06 Sartorius Gmbh Filtrationseinheit zur Abtrennung von Stoffen aus einer flüssigen Phase an Membranadsorbern
WO2002005935A2 (fr) * 2000-07-13 2002-01-24 Aaflowsystems Gmbh & Co. Kg Filtre rotatif
DE10239247C1 (de) * 2002-08-22 2003-12-24 Aaflowsystems Gmbh & Co Kg Vorrichtung zum Filtrieren von Flüssigkeiten

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4911847A (en) * 1983-12-20 1990-03-27 Membrex, Inc. Process for controlling the permeate composition in a rotary filtration device
JPH01296972A (ja) * 1988-05-25 1989-11-30 Ngk Insulators Ltd 膜型反応装置
JPH08280391A (ja) * 1995-04-18 1996-10-29 Kao Corp 発酵生産物の回収方法
DE19617775A1 (de) * 1996-05-03 1997-11-06 Sartorius Gmbh Filtrationseinheit zur Abtrennung von Stoffen aus einer flüssigen Phase an Membranadsorbern
WO2002005935A2 (fr) * 2000-07-13 2002-01-24 Aaflowsystems Gmbh & Co. Kg Filtre rotatif
DE10239247C1 (de) * 2002-08-22 2003-12-24 Aaflowsystems Gmbh & Co Kg Vorrichtung zum Filtrieren von Flüssigkeiten

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Section Ch Week 199702, Derwent World Patents Index; Class A88, AN 1997-014850, XP002460075 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210348201A1 (en) * 2020-05-06 2021-11-11 Alliance For Sustainable Energy, Llc Advanced anaerobic digestion to carboxylic acids
CN111647025A (zh) * 2020-06-18 2020-09-11 甘肃天顺植物科技有限公司 一种从柳树中提取分离精制水杨苷的方法
CN111647025B (zh) * 2020-06-18 2023-05-02 甘肃天顺植物科技有限公司 一种从柳树中提取分离精制水杨苷的方法

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Publication number Publication date
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