WO2008013995A2

WO2008013995A2 - Tridesmethylvenlafaxine and processes for the synthesis of o-desmethylvenlafaxine

Info

Publication number: WO2008013995A2
Application number: PCT/US2007/017011
Authority: WO
Inventors: Valerie Niddam-Hildesheim; Natalia Shenkar; Sharona Shachan-Tov
Original assignee: Teva Pharmaceutical Industries Ltd.; Teva Pharmaceuticals Usa, Inc.
Priority date: 2006-07-26
Filing date: 2007-07-26
Publication date: 2008-01-31
Also published as: WO2008013995A3; CA2656285A1; IL196269A0

Abstract

The present invention describes processes for the preparation of O-desmethylvenlafaxine and tridesmethylvenlafaxine, which may be used as an intermediate in preparing O-desmethylvenlafaxine.

Description

PROCESSES FOR THE SYNTHESIS OF O-DESMETHYLVENLAFAXINE

CROSS REFERENCE TO RELATED APPLICATIONS [1] The present application claims the benefit of the following United

States Provisional Patent Application Nos.: 60/833,616, filed July 26, 2006; 60/837,879, filed August 14, 2006; 60/849,216, filed October 3, 2006; 60/843,998, filed September 11, 2006; 60/849,255, filed October 3, 2006; 60/906,639, filed March 12, 2007; and 60/906,879, filed March 13, 2007. The contents of these applications are incorporated herein by reference.

FIELD OF THE INVENTION

[2] The invention encompasses a process for the synthesis of O- desmethylvenlafaxine and a novel intermediate, tridesmethyl venlafaxine.

BACKGROUND OF THE INVENTION

[3] Venlafaxine, (±)-l-[2-(Dimethylamino)-l-(4-ethyoxyphenyl) ethyl] cyclo-hexanol is the first of a class of anti-depressants. Venlafaxine acts by inhibiting re-uptake of norepinephrine and serotonin, and is an alternative to the tricyclic antidepressants and selective re-uptake inhibitors. Venlafaxine has the following chemical formula, Formula I:

Formula I

[4] O-desmethylvenlafaxine, 4-[2-(dimethylamino)-l-(l- hydroxycyclohexyl)ethyl]phenol, is a major metabolite of venlafaxine and has been reported to inhibit norepinephrine and serotonin uptake. See Klamerus, K. J. et al., "Introduction of the Composite Parameter to the Pharmacokinetics of Venlafaxine and its Active O-Desmethyl Metabolite," J. Clin. Pharmacol 32:716-724 (1992). O- desmethylvenlafaxine has the following chemical formula, Formula II:

C₁₆H₂₅NO₂ MOl. WL: 263.38

Formula II

[5] Processes for the synthesis of O-desmethylvenlafaxine, comprising a step of demethylation of the phenol group of venlafaxine, are described in U.S. patent No. 7,026,508 and 6,689,912, and in U.S. publication No. 2005/0197392. which are incorporated herein by reference.

[6] The synthesis disclosed in the above references is performed according to the following scheme:

Veπlafaxiπe VNL QDV

"MBC" refers to methyl benzyl cyanide, "CMBC" refers to cyclohexyl methylbenzyl cyanide, "DDMV" refers to didesmethyl venlafaxine, and "ODV" refers to O- desmethylvenlafaxine.

[7] However, the processes disclosed in the above US patents and US patent applications all remain problematic when applied to industrial scale production. The process in US Patent No. 7,026,508 uses L-selectride, a compound which is very problematic when scaling up the process for industrial application. Further, the process disclosed in US Application Publication No. 2005/0197392 uses lithiumdiphenyl phosphine, a compound which handling and use in industrial scale processes is extremely dangerous. Also, the process disclosed in US Patent No 6,689,912 uses methanol as a solvent, which use is problematic when traces of methanol remain and in subsequent process steps when high temperatures are applied. [8] Therefore, there is a need in the art for a new synthetic route for obtaining O-desmethylvenlafaxine, using a precursor of venlafaxine to directly obtain O-desmethylvenlafaxine.

SUMMARY QF THE INVENTION

[9] In one embodiment, the invention encompasses tridesmethyl venlafaxine.

[10] In another embodiment, the invention encompasses a process for preparing tridesmethyl venlafaxine comprising demethylating didesmethylvenlafaxine to obtain tridesmethylvenlafaxine. The process of demethylating didesmethylvenlafaxine preferably comprises: combining didesmethylvenlafaxine, a solvent, and a sulfide containing demethylating agent to form a mixture, heating the mixture, and optionally recovering tridesmethyl venlafaxine from the mixture. [11] In another embodiment, the present invention provides a process for preparing O-desmethylvenlafaxine comprising demethylating didesmethylvenlafaxine to obtain tridesmethyl venlafaxine, and converting said tridesmethyl venlafaxine to O- desmethylvenlafaxine..

[12] In another embodiment, the present invention provides a process for preparing O-desmethylvenlafaxine comprising reductive amination of tridesmethylvenlafaxine to obtain O-desmethylvenlafaxine. The process of reductive amination of tridesmethylvenlafaxine preferably comprises: combining a solution of tridesmethyl venlafaxine and a formaldehyde source with a reducing agent, preferably sodium borohydride, sodium triacetoxy borohydride, or sodium cyanoborohydride, to obtain a reaction mixture, and recovering the O-desmethylvenlafaxine from the reaction mixture.

[13] In another embodiment, the present invention provides a process for preparing O-desmethylvenlafaxine comprising selectively N₅N methylating tridesmethylvenlafaxine to obtain O-desmethylvenlafaxine. The process of selectively N,N methylating tridesmethylvenlafaxine preferably comprises: combining tridesmethyl venlafaxine, an organic solvent, and a methylating agent to form a mixture, and recovering the O-desmethylvenlafaxine from the mixture.

DETAILED DESCRIPTION OF THE INVENTION

[14] The embodiment of the invention encompasses a new synthetic route for obtaining O-desmethylvenlafaxine directly from a venlafaxine intermediate. [15] In a process according to the invention, the methoxy group of didesmethyl venlafaxine ("DDMV"), its chemical name being l-[2-amino-l-(4- methoxyphenyl)ethyl]cyclohexanol, is demethylated to form the intermediate tridesmethyl venlafaxine ("TDMV"), its chemical name being 4-[2-amino-l-(l- hydroxycyclohexyl)ethyl]phenol. The intermediate TDMV is then subjected to reductive amination or selective N₅N methylation to produce O-desmethylvenlafaxine ("ODV"), as described in the following scheme:

QDMV TDMV

QDV ' wherein "TDMV" refers to the novel intermediate tridesmethyl venlafaxine.

[16] As used herein the term "room temperature" means the ambient temperature of an typical laboratory, which is usually about that of Standard

Temperature and Pressure (STP).

[17] As used herein the term "increased pressure" refers to a pressure above

1 atmosphere as is commonly understood by one of skill in the art. Conversely, as used herein, the term "reduced pressure" means a pressure of below 1 atmosphere as commonly understood by one of skill in the art.

[18] As used herein, the term "substantially pure" means a compound of very high purity as is understood by one of skill in the art such as for example a purity of at least 95% by HPLC area percent. [19] As used herein, an "isolated" compound means the compound has been separated from the reaction mixture in which it was formed. [20] In one embodiment, the present invention provides tridesmethyl venlafaxine, chemically named 4-[2-amino-l-(l-hydroxycyclohexyl)ethyl] phenol and having the following Formula III:

tridesmethyl venlafaxine Formula III

[21] The tridesmethyl venlafaxine or salts thereof as in the present invention may have a purity of 75% by HPLC area percent or higher. Preferably, tridesmethyl venlafaxine or its salt is substantially pure, more preferably at least 95% pure by HPLC area percent, most preferably at least 98% pure by HPLC area percent. [22] In one embodiment tridesmethyl venlafaxine is prepared by demethylating didesmethyl venlafaxine to obtain tridesmethylvenlafaxine. Demethylation of disdesmethylvenlafaxine may be carried out by reacting didesmethyl venlafaxine with a sulfide containing demethylating agent. This reaction comprises maintaining a mixture of didesmethyl venlafaxine and the sulfide containing demethylating agent at an elevated temperature for a sufficient time to form tridesmethyl venlafaxine.

[23] As used herein, the term "elevated temperature" means a temperature greater than about 50⁰C, but less than a temperature at which about 10% or more of either the reactants or the product degrades over the course of the reaction. Preferably, the elevated temperature at which the demethylating reaction of the process of the present invention is carried out is from about 100⁰C to about 300°C, more preferably from about 120⁰C to about 250⁰C, even more preferably from about 140⁰C to about 210⁰C, at atmospheric pressure. Alternatively, the demethylating reaction of didesmethylvenlafaxine in the presence of a sulfide containing demethylating agent may be carried out a correspondingly lower temperature under increased pressure.

[24] Preferably, tridesmethyl venlafaxine may be prepared by a process comprising combining didesmethylvenlafaxine, a solvent, and a sulfide containing demethylating agent to form a mixture, heating the mixture, and optionally recovering tridesmethyl venlafaxine from the mixture.

[25] A suitable solvent for use in the above process may be a high boiling point solvent, particularly when the process is carried out at atmospheric pressure. The term "high boiling point solvent" is used and understood by one of ordinary skill in the art and refers to a solvent having a boiling point higher than about 100⁰C. Preferably, the high boiling point solvent is selected from the group consisting of: toluene, dimethylformamide ("DMF"), dimethylsulfoxide ("DMSO"), N-methyl-2- pyridone, N-methyl-2-pyrrolidone (NMP), l-methyl-2-pyrolidinone, dimethylacetamide ("DMA"), polyethylene glycol, Marlotherm, silicon oil, N₅N'- dimethylpropyleneurea ("DMPU"), dimethylolethyleneurea ("DMEU"), Hexamethylphosphoramide ("HMPA"), diethylformamide ("DEF"), diethyleneamine ("DEA"), morpholine, sulfolane, phenylether and mixtures thereof. More preferably, the high boiling point solvent is polyethylene glycol, NMP or DMA. [26] Preferably, the didesmethyl venlafaxine starting material is didesmethyl venlafaxine free base, which can be obtained by any method known to the skilled artisan, such as described in U.S. patent No. 7,026,508 and U.S. patent No. 6,689,912, herein incorporated by reference, or by conversion of the commercially available salt to the free base form. Such conversion may comprise dissolving a commercially available salt of didesmethylvenlafaxine, such as a hydrochloride salt or acetate salt thereof, in an organic solution, preferably a Ci -₄ alcohol such as methanol, and adding a base such as for example sodium hydroxide to the solution. The didesmethylvanlafaxine free base may then be recovered, for example, by evaporation of the solvent. Alternatively, a salt of didesmethylvenlafaxine may be used as starting material without prior conversion to the free base. The free base of didesmethyl venlafaxine may then be prepared in situ by the addition of a base. [27] The sulfide containing demethylating agent is selected from metal sulfides, having either a valence of -1 or -2, thiolates and thiols. Preferably, the demethylating agent is a mercaptan or a salt thereof, a salt of a thioalcohol, or sodium sulfide. A preferred thiolate is a high molecular weight thiolate or arene thiolate. More preferably, the sulfide containing demethylating agent is sodium dodecanethiolate or thiophenol. The sodium dodecanethiolate can be obtained by any method known to the skilled artisan, such as combining sodium methoxide, methanol and dodecanethiol.

[28] Whenever thiophenol is used, a base catalyst is pτeferably employed in the reaction mixture. Preferably, the base catalyst is a metal carbonate, hydride, hydroxide, amide or oxide. More preferably the base catalyst is selected from the group consisting OfK₂CO₃, Li₂CO₃, Na₂CO₃, Cs₂CO₃, MgCO₃, CaCO₃, BaCO₃, SrCO₃, ZnCO₃, NaHCO₃, KHCO₃, LiOH, NaOH, CsOH, LiH, NaH, KH, CaH₂, LiNH₂, NaNH₂, and tBuOK, most preferably potassium carbonate. [29] Preferably, the mixture is heated to a temperature of about 10O⁰C to about 300⁰C, preferably of about 140⁰C to about 210⁰C, preferably of about 155°C to about 190⁰C, at atmospheric pressure. The reaction mixture may be heated to a temperature of about 50⁰C to about 200⁰C, preferably about 80⁰C, whenever the demethylating reaction is carried out under pressure (increased pressure). Under pressure, the reaction may be carried out an increased pressure of more than 1 atmosphere, preferably at a pressure between about 1 bar to about 10 bar. The mixture is heated for a sufficient period of time to obtain the tridesmethylvenlafaxine, preferably for a period of about 1 hour to about 12 hours, more preferably of about 2 hours to about 6 hours, even more preferably for a period of about 2.5 hours to about 5.5 hours.

[30] The tridesmethyl venlafaxine may be recovered from the mixture by any method known to the skilled artisan. In one embodiment, recovery of tridesmethylvenlafaxine from the mixture comprises the steps of cooling the mixture; slurrying the obtained cooled mixture, preferably by adding silica; filtering and washing the slurry with a C₁-C₄ alcohol, preferably isopropanol; suspending the slurry in a C₁-C₄ alcohol, preferably isopropanol, and adjusting the pH to pH 8; filtering the suspension; and evaporating the solvent from the filtrate.

[31] In order to yield an even purer product, TDMV recovered as described above may then be slurried in water at ambient temperature for about 10 minutes to about 24 hours, preferably about 2 hours, preferably followed by removal of the water and preferably washing of the obtained product with water. TDMV so obtained is then preferably filtered and dried to yield crystalline TDMV. Optionally, the slurry may be cooled to about 0⁰C. [32] In another embodiment, the present invention provides a process for preparing O-desmethylvenlafaxine comprising demethylating didesmethylvenlafaxine to obtain tridesmethyl venlafaxine, and converting said tridesmethyl venlafaxine to O- desmethylvenlafaxine.

[33] In another embodiment, the present invention provides a process for preparing O-desmethylvenlafaxine or a salt thereof comprising reductive amination of tridesmethyl venlafaxine to obtain O-desmethylvenlafaxine. The process of reductive amination of tridesmethylvenlafaxine preferably comprises: combining a solution of tridesmethyl venlafaxine and a formaldehyde source with a reducing agent to obtain a reaction mixture and recovering the O-desmethylvenlafaxine from the reaction mixture.

[34] The tridesmethyl venlafaxine starting material may be provided in a solution with a suitable solvent, preferably an organic solvent such as C_1-4 alcohol, preferably methanol or isopropanol, or a C₁^ carboxylic acid, preferably acetic acid or formic acid, or Ce-C₈ aromatic hydrocarbons, preferably toluene, or C₃-C₅ ketones, preferably acetone and mixtures thereof. Additional solvents that can be used are also

ISIMP and DMF. Alternatively, the suitable solvent can be water.

[35] Optionally, the process is performed under acidic conditions. If the solvent used is not already acidic, an inorganic acid, such as HCl, or organic acid is added, preferably a C ₁.₆ carboxylic acid, more preferably formic acid or an acetic acid.

[36] The desired N,N-dimethylation of tridesmethylvenlafaxine may be carried out using an aldehyde, a preferred aldehyde being formaldehyde. Any source of formaldehyde can be used, such as gaseous formaldehyde, paraformaldehyde

("paraform"), a formalin solution, and trioxane to mention just a few of those known to one of ordinary skill in the art.

[37] A suitable reducing agent is selected from the group consisting of sodium borohydride, sodium triacetoxy borohydride, and sodium cyanoborohydride.

Prior to combining the reducing agent, the solution may be cooled to a temperature of less than about 10⁰C, preferably less than about 5°C, more preferably to a temperature between about 0⁰C and about 5°C.

[38] The O-desmethylvenlafaxine may be recovered from the reaction mixture by any method known to the skilled artisan.

[39] In another embodiment, the present invention provides a process for preparing O-desmethylvenlafaxine comprising selectively N₅N methylating tridesmethylvenlafaxine to obtain O-desmethylvenlafaxine. The process of selectively N₅N methylating tridesmethylvenlafaxine preferably comprises: combining tridesmethyl venlafaxine and a methylating agent, preferably with an organic solvent, to form a mixture, and recovering the O-desmethylvenlafaxine from the mixture.

[40] A preferred organic solvent is selected from the group consisting of dichloromethane, dimethylsulfoxide, acetonitrile, tetrahydrofuran, diethylether, and hexane.

[41] Optionally, the process is performed under basic conditions.

Preferably, the source for providing basic reaction conditions may be selected from the group consisting of butyllithium, triethylamine, and sodium hydride.

[42] A preferred methylating agent is selected from the group consisting of a methyl halide, preferably methyl iodide, and dimethylsulfate.

[43] The reaction may be carried out for a period of time sufficient to obtain

O-desmethylvenlafaxine. A "sufficient" amount of time depends in part on the desired extent of reaction and the reaction conditions, such as temperature. One of ordinary skill in the art can easily monitor the reaction to determine when a sufficient amount of time has transpired. The preferred amount of time is generally about 30 minutes to about 24 hours, preferably about 18 hours.

[44] The O-desmethylvenlafaxine may be recovered from the mixture by any method known to the skilled artisan.

[45] Having described the invention with reference to certain preferred embodiments, other embodiments will become apparent to one skilled in the art from consideration of the specification. The invention is further defined by reference to the following examples describing in detail the synthesis of the compound tridesmethyl venlafaxine and further its conversion to O-desmethylvenlafaxine. It will be apparent to those skilled in the art that many modifications, both to materials and methods, may be practiced without departing from the scope of the invention.

EXAMPLES

Example 1 : Determining the purity/impurity profile of tridesmethyl venlafaxine and Q-desmethylvenlafaxine by HPLC. HPLC Column & Packing: Zorbax SB C-18 4.6*250tnm Part No.28105-020 or equivalent column

Column Temperature: 25°C Buffer Add 4.0ml of trifluoroacetic acid and 7.0ml of triethylamine to IL of water adjust the pH to3.0 with triethylamine.

Eluent:

Reservoir A 30%Acetonitrile and 70% Buffer Reservoir B To a mixture of 700ml Acetonitrile and 300ml buffer add 1.6ml of trifluoroacetic acid and 2.9ml of triethylamine measure the pH it should be about 3.0 (correct the pH with triethylamine or trifluoroacetic acid if necessary).

Gradient Time Reservoir A Reservoir B

0 100% 0%

21 min 100% 0%

55 min 45% 55%

Equilibrium time: lOmin

Flow Rate: 1.0 ml/min

Detector: 230 urn

Sample Volume: 10 μl

Diluent: Eluent A

Mobile phase composition and flow rate may be varied in order to achieve the required system suitability.

Sample Preparation

Weigh accurately about 10 mg of sample in a 20ml amber volumetric flask. Dissolve with eluent A.

Method Inject the sample solutions into the chromatograph, continuing the chromatogram of sample up to the end of the gradient. Determine the areas for each peak in each solution using a suitable integrator.

Calculation

Impurity Profile Determination n, . ._^ area impurity in sample , _n_

% impurity = — x 100

Total area

Example 2: Preparation of tridesmethyl venlafaxine

1) Neutralization of Didesmethylvenlafaxine hydrochloride ("DDMVxHCl") [46] DDMVxHCl (5.73 g, 20 mmol) was dissolved in a minimum volume of methanol, and sodium hydroxide (0.88 g, 22 mmol) was added to form a mixture. The mixture was stirred at room temperature for 15 minutes. The solvent was then evaporated under reduced pressure at 90⁰C.

2) Preparation of sodium dodecanethiolate

[47] In another flask, sodium methoxide (1.43 g, 26 mmol) was dissolved in

10 ml methanol, and dodecanethiol (6.5 ml, 27 mmol) was added. The resulting solution was stirred at room temperature for 15 minutes. The solvent was then evaporated under reduced pressure at 90⁰C.

3) Demethylation

[48] The DDMV free base produced in step 1) was taken in polyethylene glycol ("PEG") 400 (5 ml) and added to the flask containing sodium dodecanethiloate of step 2). Additional PEG 400 (3ml) was used to wash the flask of step 1). The resulting mixture was heated at 190⁰C with a sand bath under nitrogen flow. The internal temperature of the flask reached 155°C. The reaction was monitored by thin layer chromatography ("TLC") and determined to be complete after 2.5 hours.

Example 3: Preparation of tridesmethyl venlafaxine

1) Neutralization of Didesmethylvenlafaxine hydrochloride ("DDMVxHCl") [49] DDMVxHCl (30 g, 105 mmol) was dissolved in a minimum volume of methanol, and sodium hydroxide (6.24 g, 115 mmol) was added to form a mixture. The mixture was stirred at room temperature for 15 minutes. The solvent was then evaporated under reduced pressure at 90⁰C. Traces of methanol were evaporated by adding toluene and evaporating it at reduced pressure at 100⁰C overnight. 2) Preparation of sodium dodecanethiolate

[50] In another flask, sodium methoxide (8.1 g, 150 mmol) was dissolved in

10 ml methanol, and dodecanethiol (32.8 ml, 136.6 mmol) was added. The resulting solution was stirred at room temperature for 15 minutes. The solvent was then evaporated under reduced pressure at 90⁰C. Traces of methanol were evaporated by adding toluene and evaporating it at reduced pressure at 100⁰C for two hours.

3) Demethylation

[51] The DDMV free base produced in step 1) was taken in polyethylene glycol ("PEG") 400 (30 ml) and added to the flask containing sodium dodecanethiloate of step 2). Additional PEG 400 (3ml) was used to wash the flask of step 1). The resulting mixture was heated at 190⁰C with a sand bath under nitrogen flow. The internal temperature of the flask reached 190⁰C. The reaction was monitored by thin layer chromatography and determined to be complete after 3 hours.

4) Work Up

[52] The reaction mixture was allowed to cool. When the temperature reached 110⁰C, toluene (100 ml) was added. When the temperature reached room temperature, silica (30 g) was added and the resulting slurry stirred for 1 hour. Then the silica was filtered. The filtrate was determined to contain dodecanethiol (and methyl dodecane thioether) by TLC analysis. The silica, which contained the product, was then suspended in isopropanol (100 mL) to form a slurry. The resulting slurry was stirred at 60⁰C for 1 hour. The slurry was then filtered and the filtrate was determined to contain ODV, DDMV, and an impurity. The silica was again suspended in isopropanol (100ml) and a solution of hydrochloric acid in isopropanol was added until ρH=8. The silica was then filtered and the solvent from the filtrate was evaporated to recover pure TDMV, with a purity of 100% by HPLC area percent, yield 52%.

Example 4: Preparation of tridesmethyl venlafaxine [53] DDMVxHCl (2 g, 7 mmol), NaOMe (0.96g, 17.7 mmol), dodecanethiol (2.3 ml=1.84 g, 9 mmol) and DMA (4 ml) were mixed together and placed in roto vapor under reduced pressure in order to evaporate all traces of MeOH formed during the contact of NaOMe with dodecanethiol and DDMV .HCl. The mixture was then heated in a sand bath at ISO⁰C. (ti_n=135°C). After 2.5 hours, a sample was analyzed by HPLC, containing 36% TDMV. Example 5: Preparation of tridesmethyl venlafaxine

[54] DDMVxHCl (1 g, 4 mmol), K₂CO₃ (0.6g, 4.4 mmol), thiophenol

(0.8ml, 6 mmol) and NiVEP (4 ml) were charged in a 50 ml flask and heated in a sand bath. The temperature of the bath was kept at 210⁰C for 6 hours. HPLC analysis confirmed full consumption of DDMV. TDMV was obtained with a purity of 83.5% by HPLC area percent.

Example 6: Preparation of tridesmethyl venlafaxine

[55] DDMVxHCl (10 g, 40 mmol), K₂CO₃ (6g, 44 mmol), Thiophenol

(8ml, 60 mmol) and NMP (40 ml) were charged in a 250 ml flask equipped with magnetic stirrer, condenser and nitrogen inlet, and heated in a sand bath. The temperature of the bath was kept at 210⁰C for 5.5 hours. HPLC analysis confirmed full consumption of DDMV. TDMV was obtained with a purity of 95% by HPLC area percent.

Example 7: Prepartion of TDMV from DDMV:

[56] 1) Neutralization of DDMVxHCl.

DDMVxHCl (10 g, 0.034 mol) was dissolved in MeOH(15 ml), and NaOMe ( 2.07 g,

0.038 mol) was added. The mixture was stirred at room temperature for 30 minutes, and the solvent evaporated under reduced pressure at 9O⁰C.

2) Demethylation

DDMV free base (prepared in stepl) was taken in NMP (15 ml) and Na₂S (4.3g, 0.035 mol) was added to 250 ml flask equipped with mechanical stirrer, condenser and nitrogen inlet. The reaction mixture was heated in sand bath to 230⁰C and the reaction was monitored by HPLC.

Example 8: Preparation of TDMV underpressure

[57] A 250 ml autoclave is charged with 5g DDMV base (0.020mol), 4.41 g thiophenol (0.040mol, 2 eq) and solvent (10 ml) and catalytic amount of K₂CO3. The reaction mixture is stirred from 4O⁰C to 220⁰C and 1-10 bar pressure for 4h. The mixture is then cooled to room temperature. At ambient temperature solvent (10 ml) and water (10 ml) are added and the product is recovered to obtain TDMV. Example 9: Preparation of TDMV from DDMV:

[58] DDMV.HC1 (10 g, 35 mmol), K₂CO₃ (5.1g, 38.4 mmol), Thiophenol

(6.2ml, 52.5 mmol) and NMP (20ml) were charged in a 100 ml flask equipped with mechanical stirrer, condenser and nitrogen inlet, and were heated in a sand bath. The temperature of the reaction mixture was about 125°C±10°C for 4 hours. The reaction mixture was cooled to 9O⁰C and H₂O (50 ml) was added dropwise inducing precipitation. The slurry was cooled to 25⁰C and stirred for about 80 minutes. The solid was filtered, washed with H₂O (20 ml) and left on filter over night and dried at 40⁰C under vacuum until constant weight to give white crystalline product (98.5% area purity by HPLC). The compound so-obtained was slurried in water (50ml) at ambient temperature for 2 hours. The solid was filtered, washed with H₂O (20 ml) and left on filter overnight and dried at 40⁰C under vacuum to give crystalline product.

Example 10: Preparation of TDMV from DDMV in DMA:

[59] DDMV.HC1 (10 g, 35 mmol), K₂CO₃ (5.1g, 38.4 mmol), Thiophenol

(6.2ml, 52.5 mmol) and DMA (20ml) were charged in a 100 ml flask equipped with mechanical stirrer, condenser and nitrogen inlet, and heated in a sand bath. The temperature of the reaction mixture was about 110°C±10°C for 3 hours. The reaction mixture was cooled to 90⁰C and H₂O (50 ml) was added dropwise inducing precipitation. The slurry was cooled to 25°C and stirred for about 4 hours .The solid was filtered, washed with H2O (20 ml) and left on filter overnight (95% area purity by HPLC). The compound so-obtained was slurried in water (50ml) at ambient temperature for 2 hours, filtered, washed with H₂O (20 ml) dried at 40⁰C under vacuum to give crystalline product.

Example 11 : Preparation of TDMV from DDMV with Na₂S in NMP: [60] DDMV.HC1 (81.36 g, 284 mmol), Na2S (40.Og, 313 mrnol), mmol) and NMP (165ml) were charged in a 500 ml reactor equipped with mechanical stirrer, condenser dean stark and nitrogen inlet The reaction mixture was heated to 185°C. The reaction mixture was stirred at 185°C for 8 hours. The reaction mixture was cooled to 90⁰C succinic acid (20 g 169 mmol)in H2O (500 ml) was added dropwise inducing precipitation. The slurry was cooled to 25°C and stirred overnight .The solid was filtered, washed with H₂O (2x80 ml) and dried overnight at 50⁰C under vacuum to get TDMV (96.91% area purity by HPLC- yield 80%). Example 12: Preparation of O-desmethylvenlafaxine

[61] TDMV (0.2 g, 0.85 mmol) was dissolved in methanol. Formalin solution (0.4 ml, 5 mmol) was added and the resulting solution was cooled in an ice bath. To the cold solution, NaBEU (65 mg, 1.7 mmol) was added. After 15 min a sample was analyzed by HPLC, and determined to contain 85 % ODV by HPLC area percent.

Example 13: Preparation of O-desmethylvenlafaxine

[62] TDMV (0.2 g, 0.85 mmol) was dissolved in acetic acid (1 ml).

Formalin solution (1.5 ml, 17 mmol) was added to the solution and the solution was cooled in an ice bath. To the cold solution NaBH(OAc)₃ (65 mg, 1.7 mmol) was added, forming a slurry that could not be stirred. Acetic acid (1 mL) was added to dilute the slurry. After 15 min, a sample was analyzed by HPLC and determined to contain 36 % ODV by HPLC area percent.

Example 14: Preparation of O-desmethylvenlafaxine

[63] TDMV (0.2g, 0.85 mmol) was dissolved in dimethylsulfoxide (2.5 ml).

The resulting solution was cooled in an ice bath causing its solidification. 1.6 M butyl lithium solution in hexane (1.1 ml, 1.7 mmol) was added, and the temperature was allowed to rise to room temperature. Then methyl iodide (0.13 ml, 2.04 mmol) was added. After 30 minutes, HPLC analysis indicated the presence of ODV.

Example 15: Preparation of O-desmethylvenlafaxine

[64] TDMV (0.5 g, 2.12 mmol) was suspended in CH₂Cl₂. Methyl iodide

(0.26 ml, 4.3 mmol) and triethylamine (0.66 ml, 4.73 mmol) were added. The reaction mixture was stirred under nitrogen atmosphere at room temperature for 6 hours. At this stage methyl iodide (0.5 ml) and NEt₃ (1.2 ml) were added. The addition caused the temperature to rise. After 16 hours, HPLC analysis indicated the presence of

ODV.

Claims

What is claimed is:

1. Tridesmethyl venlafaxine.

2. The tridesmethyl venlafaxine of claim 1, wherein the tridesmethyl venlafaxine is isolated.

3. The tridesmethyl venlafaxine of any of the preceding claims, wherein the tridesmethyl venlafaxine is substantially pure.

4. The tridesmethyl venlafaxine of any of the preceding claims, wherein the purity is about 75% by weight or greater.

5. The tridesmethyl venlafaxine of claim 4, wherein the purity is about 95% by weight or greater.

6. A process for preparing the tridesmethyl venlafaxine of any of the preceding claims comprising demethylating didesmethylvenlafaxine.

7. The process of claim 6, wherein demethylating comprises reacting didesmethylvenlafaxine with a sulfide containing demethylating agent.

8. The process of claim 7, wherein the reaction of didesmethylventalfaxine with the demethylating agent comprises maintaining a mixture of didesmethylvenlafaxine and the demethylating agent in a solvent at an elevated temperature for a sufficient time to form tridesmethyl venlafaxine.

9. The process of claim 8, further comprising: combining didesmethylvenlafaxine, a high boiling point solvent, and the demethylating agent to form a mixture, and heating the mixture to a temperature of from about 100⁰C to about 300⁰C.

10. The process of claim 9, wherein the temperature is from about 140⁰C to about 210⁰C.

11. The process of claim 10, wherein the temperature is from about 155°C to about 190⁰C.

12. The process of any of claims 98 to 11 , wherein the mixture is maintained at an elevated temperature for a period of about 1 hour to about 12. hours.

13. The process of any of claims 99 to 12, wherein the high boiling point solvent is selected from the group consisting of: toluene, dimethylformarnide ("DMF"), dimethylsulfoxide ("DMSO"), N-methyl-2-pyridone, N-methyl-2- pyrrolidone (NMP), l-methyl-2-pyroiidinone, dimethylacetamide ("DMA"), polyethylene glycol, Marlotherm, silicon oil, N,N'-dimethylpropyleneurea ("DMPU"), dimethylolethyleneurea ("DMEU"), hexamethylphosphoramide ("HMPA"), diethylformamide ("DEF"), diethyleneamine ("DEA"), morpholine, sulfolane, phenylether and mixtures thereof.

14. The process of claim 13, wherein the high boiling point solvent is polyethylene glycol, NMP or DMA.

15. The process of claim 8, wherein the mixture is heated to a temperature of about 50⁰C to about 200⁰C under a pressure of about 1 bar to about 10 bar.

16. The process of claim 15, wherein the temperature is about 8O⁰C.

17. The process of any one of claims 7 to 16, wherein the sulfide containing demethylating agent is selected from metal sulfides, the sulfide having a valence of -1 or -2, thiolates and thiols.

18. The process of claim 17, wherein the demethylating agent is selected from a mercaptan, a salt of a thioalcohol, and sodium sulfide.

19. The process of claim 18, wherein the demethylating agent is a high molecular weight thiolate or arene thiolate or thiol.

20. The process of claim 19, wherein the demethylating agent is sodium dodecanethiolate or thiophenol.

21. The process of claim 20, wherein the demethylating agent is thiophenol, and further comprising adding a catalyst to the mixture.

22. The process of claim 21, wherein the catalyst is a base catalyst selected from the group consisting of metal carbonates, metal hydrides, metal hydroxides, metal amides, and metal oxides.

23. The process of claim 22, wherein the catalyst is potassium carbonate.

24. The process of any of claims 7-23, further comprising recovering tridesmethylvenlafaxine from the mixture.

25. A process for preparing O-desmethylvenlafaxine comprising reductive amination of the tridesmethyl venlafaxine ("TDMV") of any of claims 1 to 5 to produce O-desmethylvenlafaxine ("ODV").

26. The process of claim 25, comprising: combining a tridesmethyl venlafaxine and a formaldehyde source with a reducing agent to form O- desmethylvenlafaxine.

27. The process of claim 26, wherein the tridesmethyl venlafaxine is in a solution of a solvent selected from the group consisting of a Ci_-4 alcohol, a C_1-6 carboxylic acid, a C₆-Cs aromatic hydrocarbon, a C₃-C₅ ketone, NMP, DMF, and mixtures thereof.

28. The process of any of claims 2626 or 27, wherein the process is carried out under acidic conditions.

29. The process of claim 28, wherein the process is' carried out in the presence of an organic acid.

30. The process of claim 29, wherein the organic acid is formic acid or acetic acid.

31. The process of any of claims 2626 to 30, wherein the source of formaldehyde is selected from the group consisting of gaseous formaldehyde, paraformaldehyde, fomalin solution, and trioxane.

32. The process of any of claims 26 to 31 , wherein the reducing agent is selected from the group consisting of sodium borohydride, sodium triacetoxy borohydride, and sodium cyanoborohydride.

33. The process of any of claims 26 to 32, further comprising cooling the mixture of tridesmethyl venlafaxine and a formaldehyde source to a temperature of less than about 10°C, followed by combining the mixture with a reducing agent.

34. A process for preparing O-desmethylvenlafaxine by selectively N₅N methylating tridesmethylvenlafaxine comprising: combining tridesmethyl venlafaxine of any of claims 1 to 5 and a methylating agent to form O- desmethylvenlafaxine.

35. The process of claim 34, wherein the tridesmethyl venlafaxine and the methylating agent are combined with an organic solvent selected from the group consisting of dichloromethane, dimethylsulfoxide, acetonitrile, tetrahydrofuran, diethylether, and hexane.

36. The process of any of claims 3434 or 35, wherein the process is carried out under basic conditions.

37. The process of claim 36, wherein the basic conditions are provided by a base selected from the group consisting of butyllithium, triethylamine, and sodium hydride.

38. The process of any of claims 3434 to 37, wherein the methylating agent is selected from the group consisting of a methyl halide, and dimethylsulfate.

39. A process for preparing O-desmethylvenlafaxine comprising demethylating didesmethylvenlafaxine to obtain tridesmethyl venlafaxine according to any of claims 6 to 24, and converting said tridesmethyl venlafaxine to O- desmethylvenlafaxme.

40. The process of claim 39, wherein the converting step comprises reductive amination of the tridesmethyl venlafaxine to form O-desmethylvenlafaxine according to any of claims 25 to 33.

41. The process of claim 39, wherein the converting step comprises selectively N₅N methylating the tridesmethyl venlafaxine with a methylating agent to form O-desmethylvenlafaxine according to any of claims 34 to 38.

42. Use of tridesmethyl venlafaxine in a process for manufacturing O- desmethylvenlafaxine.