Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
  • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
  • C12Q1/001—Enzyme electrodes
  • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
  • C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
  • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
  • C12Q1/001—Enzyme electrodes
  • C12Q1/004—Enzyme electrodes mediator-assisted
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
  • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
  • G01N27/28—Electrolytic cell components
  • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
  • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
  • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
  • G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels

Definitions

• the present invention therefore aims to provide an electrochemical system for reliably assaying a determined biological compound in a body fluid by means of an enzyme specific for said compound according to a protocol which makes it possible to discriminate the signal of the biological compound to be assayed from the signal of other biological compounds.
• This discrimination is fundamentally based on a sensitivity of the biological compound to be assayed and interfering biological compounds as a function of temperature, which leads to different kinetics for separating the signals of each biological compound.
• the coil is connected to the measuring apparatus which will send, for example, a pulsed current which, according to a prior calibration, makes it possible to impose at least two determined temperatures allowing, by a mathematical treatment of the signals received at said temperatures to discriminate the clean signal of the biochemical compound to be dosed from that of another biochemical compound.
• FIGS. 4 and 5 represent the responses of the sensor at different temperatures as a function, respectively, of glucose or maltose concentrations
• the design of the heating device 11 makes it possible to vary the temperature at the surface of the measuring electrode 7 without appreciably influencing the temperature of the medium, preferably without influencing the temperature of the mass of the drop of the sample to be tested arranged. on the electrodes.
• the mass therefore preferably remains at room temperature.
• the heating of the place where the electrochemical reactions take place between the sample and the electrodes will therefore preferably be localized and fast.
• Figure 2 is a simplified representation of the reactions at the surface of the measuring electrode when the medium to be analyzed contains both glucose and maltose, which is a dimer of glucose.
• D M represents the diffusion coefficient of glucose
• D 0 that of maltose
• D M ⁇ that of gluconolactone
• D D1 that of maltolactone.
• K E ⁇ i is the electron transfer coefficient between pyrroloquinoline quinone (PQQ) in the active site of the enzyme and the osmium complex of the polymer structure
• K E ⁇ n that by electron jumps in the osmium polymer
• K ET E represents the electrochemical oxidation of Os ++ into Os +++ at the surface of the measuring electrode 7.
• FIG. 3 corresponds to a temperature calibration diagram, ie a diagram which makes it possible to know the temperature at the surface of the measuring electrode of the sensor as a function of a determined current applied for a known duration.
• the known method of potentiometric calibration is used by applying to the heating coil a determined voltage in pulsed mode by means of a DC / AC converter or the like.
• FIGS. 4 and 5 show graphically, respectively for glucose and for maltose, bundles of curves showing the sensor response for increasing concentrations of the analyte at the various temperatures indicated above. As can be seen, at the same concentration, the sensor response current is significantly higher for glucose than for maltose.
• FIGS. 6 and 7 the previous experiment was repeated with increasing concentrations of glucose alone (FIG. 6) and then in the presence of 0.04 mM maltose (FIG. 7).
• the sensor response is lower in the presence of maltose, which can be explained by competitive inhibition of glucose oxidation due to the blocking of the active site of the enzyme by maltose.
• the heating coil 24 is integrated in the sensor itself.
• the coil 14 is separated from the sensor by being placed in a plate 4a extending the slot 4 of the measuring apparatus 2, so that the measurement electrode 16a comes to be positioned under said coil 24 when the sensor is engaged in the slot 4.
• the sensor can have the quite traditional form designated by the reference 10 in Figure 1 1, when the determination of the glucose level is performed with glucose oxidase .

Landscapes

• Chemical & Material Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Health & Medical Sciences (AREA)
• Organic Chemistry (AREA)
• Wood Science & Technology (AREA)
• Engineering & Computer Science (AREA)
• Proteomics, Peptides & Aminoacids (AREA)
• Zoology (AREA)
• Biochemistry (AREA)
• Analytical Chemistry (AREA)
• Physics & Mathematics (AREA)
• General Health & Medical Sciences (AREA)
• Immunology (AREA)
• Molecular Biology (AREA)
• Biophysics (AREA)
• Bioinformatics & Cheminformatics (AREA)
• General Engineering & Computer Science (AREA)
• Genetics & Genomics (AREA)
• Biotechnology (AREA)
• Microbiology (AREA)
• Emergency Medicine (AREA)
• Electrochemistry (AREA)
• Pathology (AREA)
• General Physics & Mathematics (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Hematology (AREA)
• Investigating Or Analysing Biological Materials (AREA)
• Apparatus Associated With Microorganisms And Enzymes (AREA)
• Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Priority Applications (5)

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Publications (1)

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ID=37499624

Family Applications (1)

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Cited By (1)

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Citations (5)

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• 2006
  • 2006-07-25 EP EP06117822A patent/EP1882745A1/fr not_active Withdrawn
• 2007
  • 2007-07-10 EP EP07787288A patent/EP2054522A1/fr not_active Withdrawn
  • 2007-07-10 CN CNA2007800353232A patent/CN101517094A/zh active Pending
  • 2007-07-10 JP JP2009521196A patent/JP5007338B2/ja not_active Expired - Fee Related
  • 2007-07-10 US US12/375,163 patent/US8951404B2/en not_active Expired - Fee Related
  • 2007-07-10 WO PCT/EP2007/057012 patent/WO2008012197A1/fr not_active Ceased
  • 2007-07-10 CA CA002658906A patent/CA2658906A1/en not_active Abandoned
  • 2007-07-10 AU AU2007278327A patent/AU2007278327B2/en not_active Ceased

Patent Citations (5)

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