WO2007137700A3 - Mutierte dna-polymerasen mit höherer selektivität - Google Patents
Mutierte dna-polymerasen mit höherer selektivität Download PDFInfo
- Publication number
- WO2007137700A3 WO2007137700A3 PCT/EP2007/004315 EP2007004315W WO2007137700A3 WO 2007137700 A3 WO2007137700 A3 WO 2007137700A3 EP 2007004315 W EP2007004315 W EP 2007004315W WO 2007137700 A3 WO2007137700 A3 WO 2007137700A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- dna
- polymerase
- polymerases
- higher selectivity
- mutated dna
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
Abstract
Die vorliegende Erfindung betrifft eine DNA-Polymerase mit erhöhter Fehlpaarungsdiskriminierung, welche im Vergleich zur Wildtyp-Variante mindestens eine Mutation in ihrer Aminosäure-Sequenz aufweist, sowie eine Wirtszelle und ein Verfahren zur Herstellung der DNA-Polymerase. Desweiteren betrifft die vorliegende Erfindung die Verwendung der DNA-Polymerase in diagnostischen und molekularbiologischen Verfahren und ein Kit, welches die DNA-Polymerase enthält.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE200610025153 DE102006025153A1 (de) | 2006-05-30 | 2006-05-30 | Mutierte DNA-Polymerasen mit höherer Selektivität |
DE102006025153.9 | 2006-05-30 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2007137700A2 WO2007137700A2 (de) | 2007-12-06 |
WO2007137700A3 true WO2007137700A3 (de) | 2008-03-20 |
Family
ID=38650307
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2007/004315 WO2007137700A2 (de) | 2006-05-30 | 2007-05-15 | Mutierte dna-polymerasen mit höherer selektivität |
Country Status (2)
Country | Link |
---|---|
DE (1) | DE102006025153A1 (de) |
WO (1) | WO2007137700A2 (de) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
LU92320B1 (en) | 2013-12-02 | 2015-06-03 | Univ Konstanz | Mutated DNA polymerases with high selectivity and activity |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005074350A2 (de) * | 2004-02-05 | 2005-08-18 | Rheinische Friedrich-Wilhelms- Universität Bonn | Mutierte dna-polymerasen mit erhöhter fehlpaarungs-diskriminierung |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030180741A1 (en) * | 2001-12-21 | 2003-09-25 | Holly Hogrefe | High fidelity DNA polymerase compositions and uses therefor |
-
2006
- 2006-05-30 DE DE200610025153 patent/DE102006025153A1/de not_active Withdrawn
-
2007
- 2007-05-15 WO PCT/EP2007/004315 patent/WO2007137700A2/de active Application Filing
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005074350A2 (de) * | 2004-02-05 | 2005-08-18 | Rheinische Friedrich-Wilhelms- Universität Bonn | Mutierte dna-polymerasen mit erhöhter fehlpaarungs-diskriminierung |
Non-Patent Citations (5)
Title |
---|
BRAITHWAITE DAN K ET AL: "Compilation, alignment, and phylogenetic relationships of DNA polymerases", NUCLEIC ACIDS RESEARCH, IRL PRESS LTD., OXFORD, GB, vol. 21, no. 4, 1993, pages 787 - 802, XP002143036, ISSN: 0305-1048 * |
DELARUE M ET AL: "AN ATTEMPT TO UNIFY THE STRUCTURE OF POLYMERASES", PROTEIN ENGINEERING, vol. 3, no. 6, 1990, pages 461 - 468, XP009093593, ISSN: 0269-2139 * |
MINNICK T ET AL: "side chains that influence fidelity at the polymerase active site of Escherichia coli DNA polymerase I (kleenow fragment)", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM,, US, vol. 274, no. 5, 29 January 1999 (1999-01-29), pages 3067 - 3075, XP002341285, ISSN: 0021-9258 * |
STRERATH MICHAEL ET AL: "Directed DNA polymerase evolution: Effects of mutations in motif C on the mismatch-extension selectivity of Thermus aquaticus DNA polymerase", CHEMBIOCHEM, vol. 8, no. 4, March 2007 (2007-03-01), pages 395 - 401, XP009093564, ISSN: 1439-4227 * |
SUMMERER,D. ET AL., ANGEW. CHEM. INT. ED., vol. 44, 2005, pages 4712 - 4715, XP002462227 * |
Also Published As
Publication number | Publication date |
---|---|
DE102006025153A1 (de) | 2007-12-06 |
WO2007137700A2 (de) | 2007-12-06 |
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