WO2007137679A3 - Vorrichtung und substanz zur isolierung von mesenchymalen stammzellen (msc) - Google Patents

Vorrichtung und substanz zur isolierung von mesenchymalen stammzellen (msc) Download PDF

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Publication number
WO2007137679A3
WO2007137679A3 PCT/EP2007/004057 EP2007004057W WO2007137679A3 WO 2007137679 A3 WO2007137679 A3 WO 2007137679A3 EP 2007004057 W EP2007004057 W EP 2007004057W WO 2007137679 A3 WO2007137679 A3 WO 2007137679A3
Authority
WO
WIPO (PCT)
Prior art keywords
msc
substance
stem cells
mesenchymal stem
binding
Prior art date
Application number
PCT/EP2007/004057
Other languages
English (en)
French (fr)
Other versions
WO2007137679A2 (de
Inventor
Hans-Peter Wendel
Ketai Guo
Richard Schaefer
Original Assignee
Univ Eberhard Karls
Hans-Peter Wendel
Ketai Guo
Richard Schaefer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Eberhard Karls, Hans-Peter Wendel, Ketai Guo, Richard Schaefer filed Critical Univ Eberhard Karls
Priority to EP07724981A priority Critical patent/EP2027255A2/de
Publication of WO2007137679A2 publication Critical patent/WO2007137679A2/de
Publication of WO2007137679A3 publication Critical patent/WO2007137679A3/de
Priority to US12/324,698 priority patent/US20090186356A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3517—Marker; Tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/11—Applications; Uses in screening processes for the determination of target sites, i.e. of active nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function

Abstract

Die Erfindung betrifft eine Vorrichtung mit zumindest einer mit biologischem Gewebe und/oder Flüssigkeit in Kontakt kommenden Oberfläche, welche zumindest teilweise mit einer Substanz beschichtet ist, die die Bindung von mesenchymalen Stammzellen (MSC) vermittelt, ein Verfahren zur Bindung und/oder Isolierung von MSC aus biologischem Gewebe und/oder Flüssigkeit, ein Nucleinsäuremolekül, das selektiv und hochspezifisch an MSC bindet, die Verwendung des Nucleinsäuremoleküls zur Bindung und/oder Isolierung von MSC aus biologischem Gewebe und/oder Flüssigkeit, sowie ein Verfahren zur Herstellung einer eingangs genannten Vorrichtung.
PCT/EP2007/004057 2006-05-26 2007-05-08 Vorrichtung und substanz zur isolierung von mesenchymalen stammzellen (msc) WO2007137679A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP07724981A EP2027255A2 (de) 2006-05-26 2007-05-08 Vorrichtung und substanz zur isolierung von mesenchymalen stammzellen (msc)
US12/324,698 US20090186356A1 (en) 2006-05-26 2008-11-26 DEVICE AND SUBSTANCE FOR THE IMMOBILIZATION OF MESENCHYMAL STEM CELLS (MSCs)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102006026191.7 2006-05-26
DE102006026191A DE102006026191A1 (de) 2006-05-26 2006-05-26 Vorrichtung und Substanz zur Isolierung von mesenchymalen Stammzellen (MSC)

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/324,698 Continuation US20090186356A1 (en) 2006-05-26 2008-11-26 DEVICE AND SUBSTANCE FOR THE IMMOBILIZATION OF MESENCHYMAL STEM CELLS (MSCs)

Publications (2)

Publication Number Publication Date
WO2007137679A2 WO2007137679A2 (de) 2007-12-06
WO2007137679A3 true WO2007137679A3 (de) 2008-04-03

Family

ID=38622327

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/004057 WO2007137679A2 (de) 2006-05-26 2007-05-08 Vorrichtung und substanz zur isolierung von mesenchymalen stammzellen (msc)

Country Status (4)

Country Link
US (1) US20090186356A1 (de)
EP (1) EP2027255A2 (de)
DE (1) DE102006026191A1 (de)
WO (1) WO2007137679A2 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009135198A1 (en) * 2008-05-02 2009-11-05 Biotex, Inc. Biomimetic nucleic acids
EP2417249B1 (de) * 2009-04-08 2022-06-08 The Regents of The University of California Dna-zellenkonjugate
KR20100122366A (ko) * 2009-05-12 2010-11-22 삼성전자주식회사 핵산 서열의 결정을 위한 자성 입자 및 이를 이용한 핵산 서열 결정 방법
WO2014018230A2 (en) * 2012-07-25 2014-01-30 Albert Einstein College Of Medicine Of Yeshiva University Methods to isolate human mesenchymal stem cells
BR102013021701A2 (pt) * 2013-08-26 2015-07-28 Univ São Paulo Usp Polinucleotídeos quimicamente modificados e processo de produção de polinucleotídeos quimicamente modificados
CN106771191A (zh) * 2016-12-30 2017-05-31 魏乃东 一种用于人脐血间充质干细胞检测的试剂盒
CN106483289A (zh) * 2016-12-30 2017-03-08 魏乃东 一种用于人成熟骨髓间充质干细胞检测的试剂盒
JP7418729B2 (ja) * 2019-08-08 2024-01-22 国立大学法人東京農工大学 核酸アプタマー、ヒト間葉系幹細胞吸着剤、ヒト間葉系幹細胞の分離方法、ヒト間葉系幹細胞の検出方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004025293A2 (de) * 2002-09-09 2004-03-25 Eberhard-Karls-Univer Sität Tübingen Antikörper zur isolierung und/oder identifizierung mesenchymaler stammzellen und verfahren zur isolierung und/oder identifizierung mesenchymaler stammzellen

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5567588A (en) * 1990-06-11 1996-10-22 University Research Corporation Systematic evolution of ligands by exponential enrichment: Solution SELEX
US5763566A (en) * 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue SELEX
US5837539A (en) * 1990-11-16 1998-11-17 Osiris Therapeutics, Inc. Monoclonal antibodies for human mesenchymal stem cells
US6287765B1 (en) * 1998-05-20 2001-09-11 Molecular Machines, Inc. Methods for detecting and identifying single molecules
CA2489212C (en) * 2002-06-14 2012-08-14 Cartela Ab Marker for stem cells and its use
DE10258924A1 (de) * 2002-12-17 2004-07-08 Eberhard-Karls-Universität Tübingen Universitätsklinikum Mit die Adhäsion von biologischem Material vermittelnden Substanzen beschichtete Vorrichtung
DE10358407A1 (de) * 2003-12-11 2005-07-14 Cytotools Gmbh Antiapoptorisch wirksame Aptamere (zur Therapie von Herz-Kreislauf Erkrankungen)

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004025293A2 (de) * 2002-09-09 2004-03-25 Eberhard-Karls-Univer Sität Tübingen Antikörper zur isolierung und/oder identifizierung mesenchymaler stammzellen und verfahren zur isolierung und/oder identifizierung mesenchymaler stammzellen

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GUO K ET AL: "APTAMER-BASED CAPTURE MOLECULES AS A NOVEL COATING STRATEGY TO PROMOTE CELL ADHESION", JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, UNIVERSITY PRESS CAROL DAVILA, BUCHAREST, RO, vol. 9, no. 3, July 2005 (2005-07-01), pages 731 - 736, XP008066334, ISSN: 1582-1838 *
GUO KE-TAI ET AL: "A new technique for the isolation and surface immobilization of mesenchymal stem cells from whole bone marrow using high-specific DNA aptamers", STEM CELLS (MIAMISBURG), vol. 24, no. 10, October 2006 (2006-10-01), pages 2220 - 2231, XP002465469, ISSN: 1066-5099 *
REYES M ET AL: "PURIFICATION AND EX VIVO EXPANSION OF POSTNATAL HUMAN MARROW MESODERMAL PROGENITOR CELLS", BLOOD, W.B.SAUNDERS COMPANY, ORLANDO, FL, US, vol. 98, no. 9, 1 November 2001 (2001-11-01), pages 2615 - 2629, XP001153222, ISSN: 0006-4971 *

Also Published As

Publication number Publication date
US20090186356A1 (en) 2009-07-23
EP2027255A2 (de) 2009-02-25
DE102006026191A1 (de) 2007-11-29
WO2007137679A2 (de) 2007-12-06

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