WO2007107853A3 - Production of alpha-linolenic acid in sunflower - Google Patents

Production of alpha-linolenic acid in sunflower Download PDF

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Publication number
WO2007107853A3
WO2007107853A3 PCT/IB2007/000694 IB2007000694W WO2007107853A3 WO 2007107853 A3 WO2007107853 A3 WO 2007107853A3 IB 2007000694 W IB2007000694 W IB 2007000694W WO 2007107853 A3 WO2007107853 A3 WO 2007107853A3
Authority
WO
WIPO (PCT)
Prior art keywords
alpha
linolenic acid
sunflower
delta
vectors
Prior art date
Application number
PCT/IB2007/000694
Other languages
French (fr)
Other versions
WO2007107853A2 (en
Inventor
Villoo Morawala Patell
K R Rajyashri
Original Assignee
Avestha Gengraine Tech Pvt Ltd
Villoo Morawala Patell
K R Rajyashri
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Avestha Gengraine Tech Pvt Ltd, Villoo Morawala Patell, K R Rajyashri filed Critical Avestha Gengraine Tech Pvt Ltd
Publication of WO2007107853A2 publication Critical patent/WO2007107853A2/en
Publication of WO2007107853A3 publication Critical patent/WO2007107853A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The following invention relates to the method of transformation of Sunflower with Agrobacterium tumefaciens harboring the recombinant vector comprising the nucleic acid sequence encoding the enzyme, Delta- 15 desaturase required for alpha-linolenic acid biosynthesis, driven by ubiquitin promoter. Further disclosed are the nucleic acid sequences, constructs, vectors comprising the gene encoding for Delta- 15 desaturase enzyme required for the alpha-linolenic acid production in functional combination with the heterologous regulatory sequences and the host cells into which the vectors have been introduced. Further aspects of the invention relates to the selection and regeneration of the putative transformants based on a method of positive selection using Xylose as a selectable marker.
PCT/IB2007/000694 2006-03-21 2007-03-21 Production of alpha-linolenic acid in sunflower WO2007107853A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IBPCT/IB2006/000708 2006-03-21
PCT/IB2006/000708 WO2007107807A1 (en) 2006-03-21 2006-03-21 Production of alpha-linolenic acid in sunflower

Publications (2)

Publication Number Publication Date
WO2007107853A2 WO2007107853A2 (en) 2007-09-27
WO2007107853A3 true WO2007107853A3 (en) 2008-05-08

Family

ID=37192532

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/IB2006/000708 WO2007107807A1 (en) 2006-03-21 2006-03-21 Production of alpha-linolenic acid in sunflower
PCT/IB2007/000694 WO2007107853A2 (en) 2006-03-21 2007-03-21 Production of alpha-linolenic acid in sunflower

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/IB2006/000708 WO2007107807A1 (en) 2006-03-21 2006-03-21 Production of alpha-linolenic acid in sunflower

Country Status (1)

Country Link
WO (2) WO2007107807A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101945573A (en) 2007-12-20 2011-01-12 陶氏益农公司 Low saturated-fat sunflower and associated methods
CN112603881A (en) * 2020-12-23 2021-04-06 黄景添 High-permeability moisturizing essence and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6459018B1 (en) * 1998-06-12 2002-10-01 Monsanto Technology Llc Polyunsaturated fatty acids in plants
WO2005047479A2 (en) * 2003-11-12 2005-05-26 E.I. Dupont De Nemours And Company Delta-15 desaturases suitable for altering levels of polyunsaturated fatty acids in oilseed plants and oleaginous yeast
WO2005063988A1 (en) * 2003-12-23 2005-07-14 Pioneer Hi-Bred International, Inc. Alteration of oil traits in plants
WO2006064317A1 (en) * 2004-12-14 2006-06-22 Avestha Gengraine Technologies Pvt. Ltd. Recombinant production docosahexaenoic acid (dha) in yeast

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0616644T3 (en) * 1991-12-04 2003-10-27 Du Pont Fatty acid desaturase genes from plants

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6459018B1 (en) * 1998-06-12 2002-10-01 Monsanto Technology Llc Polyunsaturated fatty acids in plants
WO2005047479A2 (en) * 2003-11-12 2005-05-26 E.I. Dupont De Nemours And Company Delta-15 desaturases suitable for altering levels of polyunsaturated fatty acids in oilseed plants and oleaginous yeast
WO2005063988A1 (en) * 2003-12-23 2005-07-14 Pioneer Hi-Bred International, Inc. Alteration of oil traits in plants
WO2006064317A1 (en) * 2004-12-14 2006-06-22 Avestha Gengraine Technologies Pvt. Ltd. Recombinant production docosahexaenoic acid (dha) in yeast

Also Published As

Publication number Publication date
WO2007107853A2 (en) 2007-09-27
WO2007107807A1 (en) 2007-09-27

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