WO2010064861A3 - Promoter variant and method for protein production using same - Google Patents

Promoter variant and method for protein production using same Download PDF

Info

Publication number
WO2010064861A3
WO2010064861A3 PCT/KR2009/007209 KR2009007209W WO2010064861A3 WO 2010064861 A3 WO2010064861 A3 WO 2010064861A3 KR 2009007209 W KR2009007209 W KR 2009007209W WO 2010064861 A3 WO2010064861 A3 WO 2010064861A3
Authority
WO
WIPO (PCT)
Prior art keywords
vector
region
promoter variant
protein production
host cell
Prior art date
Application number
PCT/KR2009/007209
Other languages
French (fr)
Korean (ko)
Other versions
WO2010064861A2 (en
Inventor
최수근
반재구
박승환
이수진
Original Assignee
한국생명공학연구원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 한국생명공학연구원 filed Critical 한국생명공학연구원
Publication of WO2010064861A2 publication Critical patent/WO2010064861A2/en
Publication of WO2010064861A3 publication Critical patent/WO2010064861A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal protein (delta-endotoxin)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to a promoter variant of Bacillus thuringiensis cry3Aa, and more particularly, to a polynucleotide having the enhanced expression rate of downstream genes by the mutation of at least one region from among the -35 region, space region, and -10 region of a Bacillus thuringiensis cry3Aa promoter, to a vector comprising the sequence of the polynucleotide, to a host cell comprising the vector, and to a method for protein production comprising (a) a step of preparing a vector comprising a cry3Aa promoter variant, (b) a step of introducing the prepared vector into a host cell, and (c) a step of recovering a target protein from the host cell.
PCT/KR2009/007209 2008-12-03 2009-12-03 Promoter variant and method for protein production using same WO2010064861A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2008-0122155 2008-12-03
KR1020080122155A KR20100063579A (en) 2008-12-03 2008-12-03 A promoter variant and a method for protein production using the same

Publications (2)

Publication Number Publication Date
WO2010064861A2 WO2010064861A2 (en) 2010-06-10
WO2010064861A3 true WO2010064861A3 (en) 2010-09-10

Family

ID=42233746

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/007209 WO2010064861A2 (en) 2008-12-03 2009-12-03 Promoter variant and method for protein production using same

Country Status (2)

Country Link
KR (1) KR20100063579A (en)
WO (1) WO2010064861A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101280503B1 (en) * 2010-02-03 2013-07-02 한국생명공학연구원 A promoter variant and a method for protein production using the same
KR101641578B1 (en) * 2014-01-08 2016-07-21 한국생명공학연구원 A promoter variant and a method for protein production using the same
KR20230091593A (en) * 2021-12-16 2023-06-23 경북대학교 산학협력단 Protein automatic expression system

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020165139A1 (en) * 2000-06-29 2002-11-07 University Of Maryland, College Park Construction of a structural variant of sublancin to facilitate its isolation and use in bioremediation of environmental contamination by gram-positive spore formers such as Bacillus anthrasis
US20050112603A1 (en) * 2003-11-21 2005-05-26 Industrial Technology Research Institute Method for purification, modification and immobilization of recombinant protein
WO2008140615A2 (en) * 2006-12-21 2008-11-20 Novozymes, Inc. Modified messenger rna stabilizing sequences for expressing genes in bacterial cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020165139A1 (en) * 2000-06-29 2002-11-07 University Of Maryland, College Park Construction of a structural variant of sublancin to facilitate its isolation and use in bioremediation of environmental contamination by gram-positive spore formers such as Bacillus anthrasis
US20050112603A1 (en) * 2003-11-21 2005-05-26 Industrial Technology Research Institute Method for purification, modification and immobilization of recombinant protein
WO2008140615A2 (en) * 2006-12-21 2008-11-20 Novozymes, Inc. Modified messenger rna stabilizing sequences for expressing genes in bacterial cells

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
AGAISSE, H. ET AL.: "Structural and Functional Analysis of the Promoter Region Involved in Full Expression of the cryIIIA toxin gene of Bacillus thuringiensis", MOL. MICROBIOL., vol. 13, no. 1, 1994, pages 97 - 107 *
BIEDENDIEK, R. ET AL.: "Plasmid System for the Intracellular Production and Purification of Affinity-Tagged Proteins in Bacillus megaterium", BIOTECHNOL. BIOENG., vol. 96, no. 3, 15 February 2007 (2007-02-15), pages 525 - 537 *

Also Published As

Publication number Publication date
WO2010064861A2 (en) 2010-06-10
KR20100063579A (en) 2010-06-11

Similar Documents

Publication Publication Date Title
WO2011053957A3 (en) Compositions and methods for the regulation of multiple genes of interest in a cell
WO2009137145A3 (en) Transcriptional control in alicyclobacillus acidocaldarius and associated genes, proteins, and methods
WO2007107302A3 (en) Novel genes encoding insecticidal proteins
WO2008104890A3 (en) Compositions and methods for producing apolipoprotein
WO2007095201A3 (en) Pseudotyped retroviral vectors and methods of use thereof
WO2013049121A3 (en) Recombinant phage and methods
MY156855A (en) Novel regulatory elements
WO2011139994A3 (en) Genetic elements, proteins, and associated methods including application of additional genetic information to gram (+) thermoacidophiles
MX2009008021A (en) Production of a lipid acyltransferase from transformed bacillus licheniformis cells.
WO2011057248A3 (en) Methods of propagating monkey adenoviral vectors
WO2008065200A8 (en) Dnase expression in recombinant host cells
MX2019003360A (en) Expression vector for an improved protein secretion.
WO2014203261A3 (en) Recombinant lectin and method of preparing thereof
MX2010001491A (en) Thiopeptide precursor protein, gene encoding it and uses thereof.
WO2014150449A3 (en) Constitutive soybean promoters
WO2008102421A1 (en) Recombinant microorganism
WO2010064861A3 (en) Promoter variant and method for protein production using same
MX2010009071A (en) Isolated peptides having phospholipase inhibitory activity.
WO2009025470A3 (en) Novel corynebacterium glutamicum promoter
WO2010127219A3 (en) Altering enzyme balance through fermentation conditions
WO2010098561A3 (en) Novel beta-galactosidase having a high transglycosylation activity, and a use therefor
WO2009112587A3 (en) Variation of recombinant expression titres by optimising bacterial ribosome binding sites
GB201205796D0 (en) Bacterial expression system
WO2013175491A3 (en) Stable expression system for eukaryotic cells
WO2008085956A3 (en) High expression cell line that eliminates gene amplification

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 09830599

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 09830599

Country of ref document: EP

Kind code of ref document: A2