WO2014203261A3 - Recombinant lectin and method of preparing thereof - Google Patents

Recombinant lectin and method of preparing thereof Download PDF

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Publication number
WO2014203261A3
WO2014203261A3 PCT/IN2010/000056 IN2010000056W WO2014203261A3 WO 2014203261 A3 WO2014203261 A3 WO 2014203261A3 IN 2010000056 W IN2010000056 W IN 2010000056W WO 2014203261 A3 WO2014203261 A3 WO 2014203261A3
Authority
WO
WIPO (PCT)
Prior art keywords
preparing
lectin
host cell
recombinant lectin
recombinant
Prior art date
Application number
PCT/IN2010/000056
Other languages
French (fr)
Other versions
WO2014203261A2 (en
Inventor
Bale Murugi Swamy
Shashikala Ramchandra Inamdar
Hemalatha Venkat
Radhika S.
Vishwanath Basavaraj Chachadi
Nagaraja Narayan Nagre
Sathisha J. GONCHIGAR
Vinita MORYE
Candalai Seshadri RAMADOSS
Original Assignee
Unichem Laboratories Ltd.
Karnatak University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unichem Laboratories Ltd., Karnatak University filed Critical Unichem Laboratories Ltd.
Priority to PCT/IN2010/000056 priority Critical patent/WO2014203261A2/en
Publication of WO2014203261A2 publication Critical patent/WO2014203261A2/en
Publication of WO2014203261A3 publication Critical patent/WO2014203261A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/375—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Basidiomycetes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention provides a method for preparing a recombinant lectin expressed in a host cell such as E.coli or yeast, said method comprising the synthesis of a lectin gene based on amino acid sequence derived from the MALDI MS/MS of the Sclerotium rolfsii lectin (SEQ ID.1), a soil borne phytopathogenic fungus, and its cloning in the host cell.
PCT/IN2010/000056 2010-02-02 2010-02-02 Recombinant lectin and method of preparing thereof WO2014203261A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/IN2010/000056 WO2014203261A2 (en) 2010-02-02 2010-02-02 Recombinant lectin and method of preparing thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IN2010/000056 WO2014203261A2 (en) 2010-02-02 2010-02-02 Recombinant lectin and method of preparing thereof

Publications (2)

Publication Number Publication Date
WO2014203261A2 WO2014203261A2 (en) 2014-12-24
WO2014203261A3 true WO2014203261A3 (en) 2015-06-04

Family

ID=52004016

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2010/000056 WO2014203261A2 (en) 2010-02-02 2010-02-02 Recombinant lectin and method of preparing thereof

Country Status (1)

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WO (1) WO2014203261A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3108842A1 (en) 2018-08-31 2020-03-05 Unichem Laboratories Limited Recombinant lectin variants
MA53554A (en) 2018-09-07 2021-07-14 Unichem Lab Ltd IMPROVED METHOD FOR THE PREPARATION OF A RECOMBINANT LECTIN PROTEIN
CN113286604B (en) * 2018-09-16 2023-05-12 联合化学实验室有限公司 Protein for treating inflammatory diseases
AU2019383003A1 (en) * 2018-11-19 2021-06-03 Unichem Laboratories Ltd Combination of chemotherapy with recombinant S. rolfsii lectin
MX2021015426A (en) 2019-07-09 2022-01-24 Unichem Lab Ltd Stable formulations of recombinant proteins.
KR20230008072A (en) 2020-05-07 2023-01-13 유니켐 레버러토리스 리미티드 anti-cancer protein
EP4366699A1 (en) 2021-07-08 2024-05-15 Unichem Laboratories Ltd Recombinant proteins, compositions and methods of stabilization thereof
CA3227834A1 (en) 2021-08-30 2023-03-09 Dhananjay Sathe Protein compositions for the treatment of inflammatory diseases
CA3229988A1 (en) 2021-10-01 2023-04-06 Dhananjay Sathe Recombinant glycan binding proteins and its use
AU2022410688A1 (en) 2021-12-16 2024-06-13 Unichem Laboratories Limited Recombinant protein for treating sars-cov2 disease

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010095143A2 (en) * 2009-02-18 2010-08-26 Unichem Laboratories Ltd Cancer cell binding recombinant lectins with antitumor activity and method of preparation

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6958321B2 (en) 2001-05-03 2005-10-25 The Regents Of The University Of Michigan Isolation, characterization, cloning and use of a mushroom lectin

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010095143A2 (en) * 2009-02-18 2010-08-26 Unichem Laboratories Ltd Cancer cell binding recombinant lectins with antitumor activity and method of preparation

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
LEONIDAS ET AL: "Structural Basis for the Carbohydrate Recognition of the Sclerotium rolfsii Lectin", JOURNAL OF MOLECULAR BIOLOGY, ACADEMIC PRESS, UNITED KINGDOM, vol. 368, no. 4, 17 April 2007 (2007-04-17), pages 1145 - 1161, XP022030023, ISSN: 0022-2836, DOI: 10.1016/J.JMB.2007.02.092 *
SATHISHA G J ET AL: "X-ray sequence ambiguities of Sclerotium rolfsii lectin resolved by mass spectrometry", AMINO ACIDS ; THE FORUM FOR AMINO ACID AND PROTEIN RESEARCH, SPRINGER-VERLAG, VI, vol. 35, no. 2, 28 December 2007 (2007-12-28), pages 309 - 320, XP019634527, ISSN: 1438-2199 *
VISHWANATH B. CHACHADI ET AL: "Exquisite binding specificity of Sclerotium rolfsii lectin toward TF-related O-linked mucin-type glycans", GLYCOCONJUGATE JOURNAL, vol. 28, no. 1, 1 January 2011 (2011-01-01), pages 49 - 56, XP055178865, ISSN: 0282-0080, DOI: 10.1007/s10719-011-9323-8 *
WU ALBERT M ET AL: "Carbohydrate specificity of a lectin isolated from the fungus Sclerotium rolfsii", LIFE SCIENCES, PERGAMON PRESS, OXFORD, GB, vol. 69, no. 17, 14 September 2001 (2001-09-14), pages 2039 - 2050, XP002610173, ISSN: 0024-3205 *

Also Published As

Publication number Publication date
WO2014203261A2 (en) 2014-12-24

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