WO2007104816A3 - Método de análisis de ácidos nucleicos - Google Patents

Método de análisis de ácidos nucleicos Download PDF

Info

Publication number
WO2007104816A3
WO2007104816A3 PCT/ES2007/000146 ES2007000146W WO2007104816A3 WO 2007104816 A3 WO2007104816 A3 WO 2007104816A3 ES 2007000146 W ES2007000146 W ES 2007000146W WO 2007104816 A3 WO2007104816 A3 WO 2007104816A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
analysing nucleic
analysing
nucleic acid
micromatrices
Prior art date
Application number
PCT/ES2007/000146
Other languages
English (en)
French (fr)
Other versions
WO2007104816A2 (es
Inventor
Tamara Maes
Duran Elena Aibar
Original Assignee
Oryzon Genomics Sa
Tamara Maes
Duran Elena Aibar
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oryzon Genomics Sa, Tamara Maes, Duran Elena Aibar filed Critical Oryzon Genomics Sa
Priority to JP2008558835A priority Critical patent/JP2009529864A/ja
Priority to AU2007226485A priority patent/AU2007226485A1/en
Priority to ES07730387T priority patent/ES2387113T3/es
Priority to AT07730387T priority patent/ATE510029T1/de
Priority to US12/225,008 priority patent/US20090181387A1/en
Priority to EP07730387A priority patent/EP2006393B1/en
Publication of WO2007104816A2 publication Critical patent/WO2007104816A2/es
Publication of WO2007104816A3 publication Critical patent/WO2007104816A3/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Método de análisis de ácidos nucleicos, que comprende las etapas de fraccionamiento del ácido nucleico, ligación de adaptadores y amplificación del ácido nucleico y una etapa de transcripción in vitro. Dicha invención tiene aplicación en el campo del análisis genómico de organismos mediante el uso de micromatrices de ADN.
PCT/ES2007/000146 2006-03-14 2007-03-13 Método de análisis de ácidos nucleicos WO2007104816A2 (es)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP2008558835A JP2009529864A (ja) 2006-03-14 2007-03-13 核酸の分析方法
AU2007226485A AU2007226485A1 (en) 2006-03-14 2007-03-13 Method for analysing nucleic acids
ES07730387T ES2387113T3 (es) 2006-03-14 2007-03-13 Método de ánalisis de ácidos nucleicos
AT07730387T ATE510029T1 (de) 2006-03-14 2007-03-13 Verfahren zur analyse von nukleinsäuren
US12/225,008 US20090181387A1 (en) 2006-03-14 2007-03-13 Method for Analysing Nucleic Acids
EP07730387A EP2006393B1 (en) 2006-03-14 2007-03-13 Method for analysing nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ESP200600703 2006-03-14
ES200600703A ES2301342B1 (es) 2006-03-14 2006-03-14 "metodo de analisis de acidos nucleicos".

Publications (2)

Publication Number Publication Date
WO2007104816A2 WO2007104816A2 (es) 2007-09-20
WO2007104816A3 true WO2007104816A3 (es) 2007-11-01

Family

ID=38509838

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/ES2007/000146 WO2007104816A2 (es) 2006-03-14 2007-03-13 Método de análisis de ácidos nucleicos

Country Status (7)

Country Link
US (1) US20090181387A1 (es)
EP (1) EP2006393B1 (es)
JP (1) JP2009529864A (es)
AT (1) ATE510029T1 (es)
AU (1) AU2007226485A1 (es)
ES (2) ES2301342B1 (es)
WO (1) WO2007104816A2 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2374194T3 (es) * 2007-03-30 2012-02-14 Oryzon Genomics, S.A. Método de análisis de ácido nucleico para analizar el patrón de metilación de islas cpg en diferentes muestras.
CN101889074A (zh) * 2007-10-04 2010-11-17 哈尔西恩莫尔丘勒公司 采用电子显微镜对核酸聚合物测序
WO2009115313A1 (en) * 2008-03-19 2009-09-24 Oryzon Genomics, S.A. Method and composition for methylation analysis
IT1392539B1 (it) * 2008-12-17 2012-03-09 Fond Parco Tecnologico Padano Microarray per l'identificazione della presenza o assenza di ogm in campioni comprendenti materiale vegetale.
US8691509B2 (en) 2009-04-02 2014-04-08 Fluidigm Corporation Multi-primer amplification method for barcoding of target nucleic acids
SG194745A1 (en) * 2011-05-20 2013-12-30 Fluidigm Corp Nucleic acid encoding reactions
JP2015519900A (ja) 2012-05-21 2015-07-16 フリューダイム・コーポレイション 粒子集団の単粒子解析方法及び単粒子単離方法
WO2017106777A1 (en) 2015-12-16 2017-06-22 Fluidigm Corporation High-level multiplex amplification

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000017390A1 (en) * 1998-09-18 2000-03-30 Micromet Ag Dna amplification of a single cell
WO2004081183A2 (en) * 2003-03-07 2004-09-23 Rubicon Genomics, Inc. In vitro dna immortalization and whole genome amplification using libraries generated from randomly fragmented dna

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3236295B2 (ja) * 1991-09-24 2001-12-10 ケイヘーネ・エヌ・ベー 選択的な制限断片増幅：一般的なｄｎａフィンガプリント法
DE69507646T2 (de) * 1994-11-28 1999-09-16 E.I. Du Pont De Nemours And Co., Wilmington Mikrosatelliteverbindung für detektion genetisches polymorphismen
WO1998012352A1 (en) * 1996-09-18 1998-03-26 The General Hospital Corporation Cleaved amplified rflp detection methods
US6287825B1 (en) * 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
AU1402900A (en) * 1998-11-09 2000-05-29 Methexis N.V. Restricted amplicon analysis
WO2000061801A2 (en) * 1999-04-09 2000-10-19 Keygene N.V. METHOD FOR THE DETECTION AND/OR ANALYSIS, BY MEANS OF PRIMER EXTENSION TECHNIQUES, OF SINGLE NUCLEOTIDE POLYMORPHISMS IN RESTRICTION FRAGMENTS, IN PARTICULAR IN AMPLIFIED RESTRICTION FRAGMENTS GENERATED USING AFLP$m(3)
EP1451346A2 (en) * 2001-08-16 2004-09-01 Curagen Corporation Method of labelling crnas for probing oligo-based microarrays
US20050147975A1 (en) * 2003-12-24 2005-07-07 Schembri Carol T. Methods and compositions for amplification of genomic DNA

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000017390A1 (en) * 1998-09-18 2000-03-30 Micromet Ag Dna amplification of a single cell
WO2004081183A2 (en) * 2003-03-07 2004-09-23 Rubicon Genomics, Inc. In vitro dna immortalization and whole genome amplification using libraries generated from randomly fragmented dna

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GUILLAUD-BATAILLE M. ET AL.: "Detecting single DNA copy number variations in complex genomes using one nanogram of starting DNA and BAC-array CGH", NUCLEIC ACIDS RES., vol. 32, no. 13, 2004, pages E112, XP008129740 *
HUGHES S. ET AL.: "The use of whole genome amplification in the study of human disease", PROG. BIOPHYS. MOL. BIOL., vol. 88, no. 1, 2005, pages 173 - 189, XP004651876 *
KLEIN C.A. ET AL.: "Comparative genomic hybridization, loss of heterozygosity, and DNA sequence analysis of single cells", PROC. NATL. ACAD. SCI. USA, vol. 96, 1999, pages 4494 - 4499, XP002144872 *
LIU C.L. ET AL.: "Development and validation of a T7 based liner amplification for genomic DNA", BMC GENOMICS, vol. 4, 2003, pages 19, XP021014452 *

Also Published As

Publication number Publication date
ES2301342B1 (es) 2009-05-01
AU2007226485A1 (en) 2007-09-20
EP2006393A2 (en) 2008-12-24
EP2006393B1 (en) 2011-05-18
ES2387113T3 (es) 2012-09-13
EP2006393A4 (en) 2009-11-18
ATE510029T1 (de) 2011-06-15
US20090181387A1 (en) 2009-07-16
ES2301342A1 (es) 2008-06-16
WO2007104816A2 (es) 2007-09-20
JP2009529864A (ja) 2009-08-27

Similar Documents

Publication Publication Date Title
WO2007104816A3 (es) Método de análisis de ácidos nucleicos
WO2007024798A3 (en) Apparatus, system, and method using immiscible-fluid-discrete-volumes
WO2010026488A3 (en) Methods and kits for nucleic acid sequencing
WO2011143659A3 (en) Nucleic acid isolation methods
WO2009092035A3 (en) Methods and compositions for the analysis of biological molecules
WO2009152928A3 (de) Verfahren zur quantitativen analyse von nukleinsäuren, marker dafür und deren verwendung
WO2008005321A3 (en) Systems and methods for real-time pcr
WO2006099164A3 (en) Methods for multiplex amplification
WO2007086935A3 (en) Methods of amplifying and sequencing nucleic acids
WO2010083250A3 (en) Single-cell nucleic acid analysis
WO2006102309A3 (en) Methods, compositions, and kits for detection of micro rna
WO2007123744A3 (en) Systems and devices for sequence by synthesis analysis
WO2007035684A3 (en) Method for quantitative detection of short rna molecules
EP2224017A4 (en) PROCESS FOR INCREASING THE NUCLEIC ACID TARGET SEQUENCE AND PROBE USED THEREFOR
WO2007065025A3 (en) Method of dna analysis using micro/nanochannel
WO2009121032A3 (en) Sample preparation devices and methods for processing analytes
WO2008039769A3 (en) Methods and devices for analyzing small rna molecules
WO2010147654A3 (en) Microfluid methods for forensic dna quantification
WO2006135684A3 (en) Methods and kits for sense rna synthesis
WO2007044974A3 (en) Absolute pcr quantification
WO2007019492A3 (en) Methods for detection of a nucleic acid by sequential amplification
WO2007100934A3 (en) Methods and compositions for the rapid isolation of small rna molecules
WO2007039319A3 (en) Dna microarray for rapid identification of candida albicans in blood cultures
WO2006057918A3 (en) Detection of nucleic acid variation by cleavage-amplification method
WO2009049916A3 (en) Method for determining methylation at cytosine residues

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07730387

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 2008558835

Country of ref document: JP

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2007226485

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2007730387

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 2007226485

Country of ref document: AU

Date of ref document: 20070313

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 12225008

Country of ref document: US