WO2007095359A3 - Assay for distinguishing live and dead cells - Google Patents

Assay for distinguishing live and dead cells Download PDF

Info

Publication number
WO2007095359A3
WO2007095359A3 PCT/US2007/004125 US2007004125W WO2007095359A3 WO 2007095359 A3 WO2007095359 A3 WO 2007095359A3 US 2007004125 W US2007004125 W US 2007004125W WO 2007095359 A3 WO2007095359 A3 WO 2007095359A3
Authority
WO
WIPO (PCT)
Prior art keywords
image
live
cells
indicator
dead
Prior art date
Application number
PCT/US2007/004125
Other languages
French (fr)
Other versions
WO2007095359A2 (en
Inventor
Vadim Kutsyy
Jinhong Fan
Eugeni A Vaisberg
Original Assignee
Cytokinetics Inc
Vadim Kutsyy
Jinhong Fan
Eugeni A Vaisberg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/355,258 external-priority patent/US20070031818A1/en
Application filed by Cytokinetics Inc, Vadim Kutsyy, Jinhong Fan, Eugeni A Vaisberg filed Critical Cytokinetics Inc
Publication of WO2007095359A2 publication Critical patent/WO2007095359A2/en
Publication of WO2007095359A3 publication Critical patent/WO2007095359A3/en

Links

Classifications

    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/194—Segmentation; Edge detection involving foreground-background segmentation
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro

Abstract

Image analysis methods and apparatus are used for distinguishing live and dead cells. The methods may involve segmenting an image to identify the region(s) occupied by one or more cells and determining the presence of a particular live-dead indicator feature within the region(s). In certain embodiments, the indicator feature is a cytoskeletal component such as tubulin. In certain embodiments, the methods may involve determining the value of an indicator expression that is based on cellular components such as DNA and/or cellular protein. Prior to producing an image for analysis, cells may be treated with a marker that highlights the live-dead indicator in the image.
PCT/US2007/004125 2006-02-14 2007-02-13 Assay for distinguishing live and dead cells WO2007095359A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US11/355,258 US20070031818A1 (en) 2004-07-15 2006-02-14 Assay for distinguishing live and dead cells
US11/355,258 2006-02-14
GB0604675A GB2435093A (en) 2006-02-14 2006-03-08 Assay for distinguishing live and dead cells
GB0604675.9 2006-03-08

Publications (2)

Publication Number Publication Date
WO2007095359A2 WO2007095359A2 (en) 2007-08-23
WO2007095359A3 true WO2007095359A3 (en) 2008-03-06

Family

ID=38268952

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/004125 WO2007095359A2 (en) 2006-02-14 2007-02-13 Assay for distinguishing live and dead cells

Country Status (2)

Country Link
GB (1) GB2435093A (en)
WO (1) WO2007095359A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7134184B2 (en) 2017-05-25 2022-09-09 アボット・ラボラトリーズ Method and system for sample analysis

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102009058169B4 (en) 2009-12-15 2012-06-28 Ralf, Prof. Dr. Erdmann Protein or polypeptide for binding to tubulin and / or microtubule structures
US8300938B2 (en) * 2010-04-09 2012-10-30 General Electric Company Methods for segmenting objects in images

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050272073A1 (en) * 2000-12-04 2005-12-08 Cytokinetics, Inc., A Delaware Corporation Ploidy classification method
US20060014135A1 (en) * 2004-07-15 2006-01-19 Cytokinetics, Inc. Assay for distinguishing live and dead cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050272073A1 (en) * 2000-12-04 2005-12-08 Cytokinetics, Inc., A Delaware Corporation Ploidy classification method
US20060014135A1 (en) * 2004-07-15 2006-01-19 Cytokinetics, Inc. Assay for distinguishing live and dead cells

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7134184B2 (en) 2017-05-25 2022-09-09 アボット・ラボラトリーズ Method and system for sample analysis

Also Published As

Publication number Publication date
WO2007095359A2 (en) 2007-08-23
GB2435093A (en) 2007-08-15
GB0604675D0 (en) 2006-04-19

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