WO2007089551A2 - systèmes de bandelettes réactives spectroscopiques de Raman - Google Patents

systèmes de bandelettes réactives spectroscopiques de Raman Download PDF

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Publication number
WO2007089551A2
WO2007089551A2 PCT/US2007/002106 US2007002106W WO2007089551A2 WO 2007089551 A2 WO2007089551 A2 WO 2007089551A2 US 2007002106 W US2007002106 W US 2007002106W WO 2007089551 A2 WO2007089551 A2 WO 2007089551A2
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WO
WIPO (PCT)
Prior art keywords
test strip
sample
test
radiation
substrate
Prior art date
Application number
PCT/US2007/002106
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English (en)
Other versions
WO2007089551A3 (fr
Inventor
Richard H. Clarke
E. Edward Womble
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Prescient Medical, Inc.
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Filing date
Publication date
Priority claimed from US11/340,712 external-priority patent/US7651851B2/en
Application filed by Prescient Medical, Inc. filed Critical Prescient Medical, Inc.
Publication of WO2007089551A2 publication Critical patent/WO2007089551A2/fr
Publication of WO2007089551A3 publication Critical patent/WO2007089551A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue
    • A61B5/14546—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue for measuring analytes not otherwise provided for, e.g. ions, cytochromes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N21/8483—Investigating reagent band
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B2562/00—Details of sensors; Constructional details of sensor housings or probes; Accessories for sensors
    • A61B2562/02—Details of sensors specially adapted for in-vivo measurements
    • A61B2562/0295—Strip shaped analyte sensors for apparatus classified in A61B5/145 or A61B5/157
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis

Definitions

  • the invention relates generally to diagnostic apparatuses for the optical interrogation of test strips by Raman spectroscopy.
  • United States Patent No. 6,844,200 which is incorporated by reference herein in its entirety, describes devices for carrying out lateral-flow assays involving more than one analyte that are not based on Raman spectroscopy.
  • United States Patent No. 6,924,153 which is incorporated by reference herein in its entirety, describes quantitative lateral flow assays and devices that are not based on
  • United States Publication No. 20040116829 which is incorporated by reference herein in its entirety, describes sampling devices and methods using a horizontal capillary test strip that may employ Raman spectroscopy.
  • United States Publication No. 20040186394 which is incorporated by reference herein in its entirety, describes integrated lancing test strip that may employ Raman spectroscopy.
  • United States Publication No. 20040191921 which is incorporated by reference herein in its entirety, describes simultaneous chemical separation and surface-enhanced Raman spectral detection.
  • One embodiment of the invention provides a Raman spectroscopy system that includes: an optical assembly defining an input path and an output path, wherein the input path includes at least one light directing element that receives excitation radiation from a light source and directs it to a sample and the output path further includes at least one light collecting element that collects radiation scattered by the sample for analysis, wherein the input and output paths of the optical assembly are at least substantially orthogonal and the optical assembly further includes a dichroic mirror that directs the excitation radiation to the sample by reflection and transmits scattered radiation returning from the sample to the output path; and a fluid sample, such as a body fluid sample, test substrate-receiving structure for receiving a fluid sample test substrate, which may be standardized and which may be a body fluid sample test substrate, and positioning said substrate for optical interrogation via the optical assembly.
  • a fluid sample such as a body fluid sample, test substrate-receiving structure for receiving a fluid sample test substrate, which may be standardized and which may be a body fluid sample test substrate, and positioning
  • a related embodiment of the invention provides a Raman spectroscopy system that includes: an input fiber through which radiation can propagate to irradiate a sample; a collection fiber through which radiation scattered by the sample can be coupled to a detector; an optical assembly defining an input path and an output path, wherein the input path comprises at least one light directing element that receives excitation radiation from the input fiber and directs it to the sample and the output path further includes at least light collecting element that collects radiation scattered by the sample and couples the scattered radiation into the collection fiber; the input and output paths of the optical assembly are at least substantially orthogonal and the optical assembly further comprises a dichroic mirror that directs the excitation radiation to the sample by reflection and transmits scattered radiation returning from the sample to the output path; and a fluid sample, such as a body fluid sample, test substrate-receiving structure for receiving a fluid sample test substrate, which may be standardized, and positioning said substrate for optical interrogation via the optical assembly.
  • a fluid sample such as a body fluid sample, test substrate-recei
  • the fluid sample test substrate receiving structure may include a slot for insertion of the substrate and a guiding channel into which the substrate is inserted.
  • the fluid sample test substrate-receiving structure may be a test strip-receiving structure.
  • the fluid sample test substrate is a test strip.
  • the test strip may be a lateral flow binding assay test strip.
  • the test strip may include a non-mobile surface enhanced Raman spectroscopy substrate.
  • the fluid sample test substrate receiving structure which may be a test strip receiving structure, and the substrate may be mutually adapted to alignedly position the substrate for optical interrogation by the system.
  • the optical assembly further includes a beam energy dump for absorbing radiation from the input path that is not reflected by the dichroic mirror.
  • Another embodiment of the invention provides a method for evaluating a fluid sample by Raman spectroscopy, that includes the steps of: depositing a fluid sample, such as a body fluid sample, on a test strip; inserting the test strip into a Raman spectroscopy system according to the invention, wherein the system irradiates at least one part of the test strip to generate a Raman spectra; and wherein the system analyzes at least part of the Raman spectra.
  • the test strip may include a SERS active substrate in a sample deposition area and the system irradiates the sample deposition area to generate the Raman spectra.
  • the test strip may alternatively or additionally be a lateral flow binding assay test strip including a mobile phase and the system irradiates one or more test stripes and optionally one or more control stripes of the test strip.
  • the mobile phase may include SERS-active particles.
  • the SERS- active particles may include gold beads and/or gold-coated beads.
  • FIG. 1 is a schematic diagram of an optical assembly that may be used in systems according to the invention for the optical interrogation of test substrates.
  • FIG. 2 is a partial cross-sectional schematic diagram of an optical head assembly and housing adapted for optically interrogating test substrates, according to the invention;
  • FIG. 3 is a partial cross-sectional schematic view of a sampling tube according to the invention.
  • FIG. 4 is an overall schematic view of a portable Raman spectroscopy system embodiment of the invention that is adapted for interrogating tests strips;
  • FIG. 5 is an overall schematic view of a portable Raman spectroscopy system embodiment of the invention that is adapted for interrogating tests strips, in which all of the systems components are disposed within a single housing.
  • FIG. 6 is a block diagram of a laser/analyzer assembly for a Raman spectroscopy ⁇ system according to the invention.
  • FIG. 7 shows an example of a SERS substrate-type test strip.
  • FIG.. 8 shows a lateral flow assay test strip having notches for aligning the test stripe and control stripes of the strip with the optical probe of a Raman reader unit.
  • FIG. 9 shows the lateral test strip of FIG. 8 alignedly positioned in the positioning mechanism of a Raman reader unit for reading of the test stripe by the optical probe of the reader unit.
  • FIG. 10 shows a cross section of the test strip of FIG. 9 positioned in the test strip- guiding rails of the reader unit.
  • DETAILED DESCRIPTION [00029] The invention provides Raman spectroscopy systems that are adapted to receive and read at least substantially planar sample substrates such as test strips and that include an advantageously configured optical assembly by which the sample substrates are optically interrogated. [00030] The invention is further described below with reference to the appended figures. The numeric designation of elements in each figure is not necessarily consistent between figures.
  • an optical assembly 100 as illustrated schematically in FIG. 1 is provided and can be used to perform Raman analyses of test substrates such as test strips.
  • Optical assembly 100 directs the light received from the spectroscopic components into position for contacting the sample, collects the scattered radiation, and returns the collected radiation for analysis.
  • Excitation radiation enters optical assembly 100 via optical fiber 101.
  • the beam from the input fiber is passed through lens 102, which serves to collimate or otherwise project the incoming radiation along beam path 106 with minimal dispersion.
  • the radiation from lens 102 then passes through an optional safety switch 103 including chamber 104 and through one or more optional filters 105, e.g., a low-pass filter.
  • the filtered incoming light along path 106 is then reflected by dichroic mirror 107 (which may be designed or selected to reflect at nearly all of the excitation light, at least about the desired excitation wavelength) and directed toward target area 121.
  • a second lens 108 may be disposed to focus the excitation radiation to a particular point or region within a sample 122. Lens 108 may generally focus the light on target area 121.
  • Returning radiation 109 passes through lens 108, which now serves to collimate the scattered radiation and convey it to collection fiber 120. From lens 108, the collected radiation travels along beam path 110, passing through dichroic beam-splitter 107 and, optionally, a mid-pass or long-pass filter 111 and lens 112.
  • Lens 112 serves to focus the collected radiation into output fiber 120.
  • the lens elements of the present invention can be simple or compound lens assemblies and that the functions that these optical elements perform — directing excitation radiation into a sample and collecting scattered radiation for analysis — can be achieved by various equivalent structures, such as those known in the art.
  • Optical assembly 100 can further include a "beam dump" 113 to capture and absorb incoming radiation that is not reflected by dichroic mirror 107.
  • Beam dump 113 is optional and may comprise a chamber that has been coated with suitable radiation absorbing material or otherwise formed or shaped to ensure that the radiation that is not directed into the sampling tube is captured and dissipated as heat.
  • Safety switch 103 is formed by a protective shutter, as shown in FIG. 1, that is disposed in chamber 104. Chamber 104 intersects incoming beam path 106. Plunger 114 is disposed within chamber 104 and operatively connected to spring 1 15 and solenoid 116. In an activated state, solenoid 116 pulls plunger 114 out of light beam path 106, thereby allowing the multimode radiation to pass through optical assembly 100 and to sample 122. In a deactivated state, ("laser blocking" position), the solenoid releases plunger 114, which moves into light beam path 106 and prevents the multimode radiation from passing to the outside environment. Thus, safety switch 103 ensures that the probe remains in a "normally- off * state should a malfunction or power loss occur.
  • FIG. 2 is a schematic diagram of a probe 230 including an optical head assembly 231 having a similar optical configuration as shown in FIG. 1 secured in a housing 232 which is adapted to receive a test strip for optical interrogation by the probe 230.
  • Housing 232 has a slot 233 opening into a channel 234 each being sized and configured to receive an elongate test strip.
  • Probe 230 as shown has a sampling tube 260.
  • sampling tube 260 is optional as the optical head and test strip receiving structure may also be configured to cooperate without the use of a sampling tube.
  • Excitation radiation e.g., from a light source enters the head assembly as in FIG. 1.
  • the input fiber 234 may be coupled to the head assembly 231 via a coupler 235.
  • the beam from the input fiber is passed through lens 236, which serves to collimate or other project the incoming radiation through the optical head assembly along beam path 237 with minimal dispersion.
  • the radiation from the lens 236 then passes through chamber 238 of the safety switch 239.
  • the incoming beam then passes through one or more optional filters 240, e.g., a low-pass filter.
  • the filtered incoming light is then reflected by dichroic mirror 241 (which is designed to reflect nearly all of the excitation light) and directed into the sampling tube 260.
  • a second lens 242 may be disposed to focus the excitation radiation to a particular point or target region 243 on an inserted test strip.
  • the sampling tube 260 also serves to collect radiation scattered by a sample in response to the excitation beam 244, The returning radiation 245 passes through lens 242 which now serves to collimate the scattered radiation and convey it to collection fiber 246. From the lens 242, the collected radiation travels along beam path 247, passing through dichroic mirror 241 and, optionally, a mid-pass or long-pass filter 248 and lens 249.
  • Lens 249 serves to focus the collected radiation into the output fiber 246 via output coupler 250.
  • the optical head assembly 231 may further include a "beam dump" 251 as in FIG. 1.
  • the safety switch 239 may be configured and function as described as in FIG. 1.
  • the input and output paths are at least substantially orthogonal. Probe assemblies such as those disclosed in U.S. Patent No. 6,897,951, which is incorporated by reference herein in its entirety, may be adapted for use in the systems of the present invention.
  • sampling tube 260 is shown in more detail.
  • the tube 260 can include an externally threaded portion 353 that mates with an internal threaded recess in the block 252 of the optical head assembly 230, as shown in FIG. 2.
  • Block 252 may, for example, be formed of aluminum.
  • Sampling tube 260 may be hollow and include an inner passageway 354 which is sufficiently wide so that both the incoming excitation beam and radiation collected from the sample (collectively, shown as the beam path 355) can propagate through the tube with minimal interaction with the tube walls 356.
  • lens 242 disposed in the sampling tube serves to both focus the excitation radiation and/or otherwise relay the collected radiation propagating through the tube.
  • the sampling tube may have a distal, sampling end window composed for example of glass or quartz (not shown) that seats into internally threaded portion 357.
  • FIG. 4 shows an overall Raman spectroscopy system for the analysis of test strips that includes a probe section 430 with its optical head assembly 431 and sampling tube 460 secured in a housing 432 which includes a test st ⁇ p receiving slot 433 and test st ⁇ p guiding channel 434, as well as a handheld or otherwise portable analyzer/laser assembly 465
  • the system shown includes a handheld instrument 465 having a display 466, a keyboard or other user interface 467, and one or more communication ports 468 for receiving or downloading data from other sources.
  • Input and output optical fibers are housed in a flexible conduit 470.
  • a general system embodiment of the invention includes a test substrate-receiving structure such as a test strip-receiving structure; an optical assembly as described, for example, one having at least substantially orthogonal input and output paths that mediates delivery of excitation light, for example, from a laser light source of the system, to at least one target area of the test substrate (when inserted into the receiving structure) and that mediates delivery of at least some of the Raman spectra light resulting from the irradiation to a detector that detects wavenumber-shifted light; and a processor that analyzes the detected light to provide an quantitative and/or qualitative characterization of at least one analyte in the sample.
  • a test substrate-receiving structure such as a test strip-receiving structure
  • an optical assembly as described, for example, one having at least substantially orthogonal input and output paths that mediates delivery of excitation light, for example, from a laser light source of the system, to at least one target area of the test substrate (when inserted into the receiving structure)
  • processor it is meant a microprocessor system including a microprocessor and whatever computer readable memory and computer instructions may be necessary to carry out processor controlled tasks/calculations.
  • the system may also include at least one output device such as a display and/or a printer to report the results of a test performed using the system.
  • the system may also include an input device such as a keyboard or keypad for programming the system or providing the system with instructions or otherwise controlling the system
  • FIG. 5 shows an overall Raman spectroscopy system 500 that may be used perform sampling operations on test substrates such as test strips according to the invention and which similar to that of FIG. 4, except that all of the components are housed withm a single housing 501.
  • System 500 includes a processor 534 that controls a display 543 and a laser 522, receives input from a keyboard 542 and receives and analyzes input from an onboard Raman spectroscopic element 535.
  • System 500 includes a multi-mode laser source 522 and optional relay optics 526 connected to an excitation optical fiber 524 that carries the laser light to an optical probe head 523 that includes a sampling tube 560 so that excitation light can be directed to (and scattered light received back from) a test strip substrate inserted into test strip-receiving channel 544.
  • the laser source 522 may be one of many multi-mode laser sources
  • the B&W Tek multi-mode laser BWF-OEM-785-0 5 available from B&W Tek, Inc., of Newark, Del., can be used as the multi-mode laser.
  • the laser source may be a feedback-stabilized multi-mode laser diode such as a volume Bragg grating (VBG)-stabilized multi-mode laser diodes (available from PD-LD Inc., Penmngton, NJ) or a single-mode laser, such as those known in the art.
  • VBG volume Bragg grating
  • the Raman scattered light is collected via the sampling tube 560 and the optical probe head 523 and delivered via flexible optical fiber 528 and optional relay optics 529 to a Raman spectroscopic element 535 of the system.
  • the Raman spectrometer element may, for example, be a low-resolution or high-resolution Raman spectrometer element
  • System 500 may also be generally provided with a power supply 540 and a cooling fan 541.
  • FIG. 6 schematically shows a low-resolution Raman spectroscopy apparatus 600 that includes a laser source 622, such as a multi-mode diode laser, and optical fiber 624 connected to the laser source for carrying laser light to the sampling site.
  • a laser source 622 such as a multi-mode diode laser
  • optical fiber 624 connected to the laser source for carrying laser light to the sampling site.
  • Relay optics 626 can optionally be used with the optical fiber to focus and direct the radiation.
  • optical fiber 624 may include a variety of optical fibers and light carrying materials that can collect and direct radiation. Light is preferably directed by optical fiber 624 to the sampling area via the optical probe, and after encountering a sample, such as a body fluid sample, is returned in a second optical fiber 628.
  • the returned radiation is directed through dispersion element 630 that serves to disperse the scattered light into different wavelength components.
  • Optional relay optics 629 may be provided between optical fiber 628 and dispersion element 630.
  • the dispersed scattered light is detected by photodetector array 632 that, in this case, consists of a photodiode array or a charged-coupled device (CCD) array.
  • the signals generated by detector array 632 in response to the scattered light are then sent to microprocessor 634 for analysis.
  • specific spectral bands of interest may be measured at low resolution to obtain the integrated band intensities.
  • the resolving power of the dispersion device 630 determines the position of specific wavelengths in the diode array in such a way that the signal from a particular diode in the array will typically correspond to the same (or a similar) narrow range of wavelengths.
  • the combination of the low-resolution dispersion device 630 and the diode array photodetector 632 thus form a spectrometer
  • the microprocessor 634 may select a particular diode (or diodes) of the array 632 according to the property to be measured.
  • the integrated signals lying m the two ranges can be arithmetically divided to form intensity ratios.
  • the microprocessor 634 may compare these ratios with known values or a correlating function to obtain an estimate of the chemical constituent or property of interest. [00048]
  • a high-resolution Raman spectrometer element may be used if higher resolution is desired.
  • a switch or detector may be provided that directly or indirectly prevents laser activation when a test substrate, such as a test strip is not inserted into the test substrate- receiving slot/channel.
  • the slot may be provided with a hingeable, self-closing slot door that at least substantially blocks light transmission through the slot when a test substrate, such as a test strip is not interested into the test substrate- receiving slot/channel.
  • the term "light” can refer to radiation having wavelength components that lie in the visible range of the electromagnetic spectrum, or outside the visible range, e.g., the infrared or ultraviolet range of the electromagnetic spectrum.
  • the preferred excitation wavelengths will range from about 700 nanometers to 2.5 micrometers.
  • this portion of the electromagnetic spectrum is commonly known as infrared (IR) radiation
  • the term "light” will be used as shorthand expression in describing the path of this radiation as well as the various wavelengths of radiation induced by Raman scattering and collected for analysis.
  • the systems of the invention can be implemented with any suitable sort of Raman spectroscopy. For example, high-resolution Raman systems as well as low-resolution Raman systems may be used.
  • Optical fibers that may be utilized in the apparatus of the invention may be multimode fibers, which are available from several commercial sources including, for example, Fiberguide, Inc. of Sterling, NJ. Their diameters may, for example, range from 1 micron to 1000 microns, such as from about 100 microns to about 400 microns, for example, from about 100 microns to about 200 microns. Single fibers and/or fiber bundles may be utilized in the present invention.
  • the various housings and sampling tubes may, for example, be constructed from various metallic materials, for example, aluminum, steel, copper or titanium and/or may be made from a rigid polymer. Hastalloy and/or stainless steel may, for example, be used.
  • the diameter of the sampling tube structure is selected such that it enables multi- mode radiation to pass through to the sample, and scattered radiation to pass from the sample without contacting the walls of the tubular structure.
  • the inner diameter of the sampling tube may range from about 3 mm to about 9 mm, such as from about 5 mm to about 6 mm.
  • the outer diameter of the sampling tube may range from about 10 mm to about 25 mm.
  • Raman measurements may be made in a number of ways in the implementation of the present invention, for example, by comparing the characteristic Raman lines in a spectrum of a sample, with the number of characteristic lines with the standard spectra. Another example includes comparing the ratio of intensities of two characteristic Raman lines which are common in the spectra for a particular constituent with the respective ratios with a standard of that constituent. The correlation of a previously stored spectrum may be used to determine whether the spectrum of the scattered Raman radiation is similar, and the resulting comparison may, for example, be displayed in an alphanumerical form by the microprocess or.
  • a substantially planar substrate for Raman interrogation may be provided in any shape. In a preferred embodiment, the test substrates have the form of at least substantially rectangular test strips.
  • test substrate and test strip may include not only the actual test strip material but also a casing or housing in which a test substrate or test strip is provided.
  • the test strip receiving structure of the systems of the invention may be sized and configured to receive the test strip in whatever form, with or without a housing, in which the test strip is ordinarily used.
  • the overall dimensions of a test strip may have one shape, the actual substrate material on which a sample is interrogated may have another shape.
  • a rectangular test strip may have one or more square, circular or oval sample deposition substrates along its length.
  • the tests strips may include a surface enhance Raman spectroscopy SERS-active surface, such as a roughened metallic surface, for deposition and analysis of a sample by irradiation of the sample deposition area of the strip and/or the test strips may be lateral flow binding assay type test strips and Raman interrogation may occur at one or more antigen binding stripes and control stripes.
  • a surface enhance Raman spectroscopy SERS-active surface such as a roughened metallic surface
  • test substrate such as a test strip
  • the test strip provides an area to deposit a body fluid sample onto which the laser radiation is directed, and even more preferably, includes features to enhance spectroscopy.
  • the test strip can include a substance for surface enhanced Raman spectroscopy (SERS), such as a roughened metallic surface and/or SERS-active metallic particles.
  • SERS-active metallic particles may, for example, be solid metallic particles or particles that are at least partially coated with a SERS-active metal.
  • SERS techniques enhance Raman spectroscopic signals and allow more effective differentiation of spectroscopic signatures by placing the sample to be analyzed in contact with SERS material (usually an appropriately prepared metal surface). While not being limited by theory, two mechanisms are considered responsible for the improvement. The primary contribution is an enlargement of the local electromagnetic field, due to the excitation of a localized surface plasmon, while the other mechanism results from a charge transfer-state between the surface complex of the adsorbed molecule and the metal surface.
  • a SERS test strip includes SERS-active material, such as, for example silver, gold, nickel, copper and/or cadmium.
  • SERS active test strip 700 (non-lateral flow type) including a support substrate 772 and a roughened metal surface including layer 774 having a degree of roughness sufficient to induce the SERS effect.
  • Layer 774 may include a microparticle or microstructure layer 776 on the upper surface of support substrate 772 and/or a metal layer 778 containing silver, gold, nickel, copper and/or cadmium.
  • a coating 790 can be applied to roughened surface layer 774 to sorb analytes (absorb and/or adsorb) which are not easily sorbed by the roughened surface, but which are capable of either penetrating into the coating or being attached to the coating.
  • the analytes are thereby absorbed and/or adsorbed to coating 790 and become positioned in the vicinity of the roughened surface and exhibit the SERS effect.
  • the coating may be specific or non-specific for the analyte so long as it provides the sorbing function.
  • the SERS active material is positioned only in target (sample deposit) area of the test strip.
  • a sample is deposited on the SERS-active material in the target area and light is directed toward the sample for spectroscopic analysis.
  • the deposited sample may be unprocessed or further processed before the irradiation.
  • the target zone may be washed with a buffer solution prior to irradiation to remove non- specifically binding components of the sample from the target zone.
  • the SERS-active materials include a porous sol-gel containing gold microcolloid particles where the laser radiation is about 785 nm.
  • the test substrate such as test strip
  • the lancet may include a sharpened tip and a mechanism for propelling the metal tip into a user's skin.
  • An exemplary lancet is the BD Ultra-FineTM Lancet available from BD Consumer Healthcare (Ontario, Canada).
  • One aspect of the present invention provides a method to read lateral flow immunoassay test kits quantitatively for the amount of one or more preselected antigens (or antibodies) present in the sample, using Raman spectroscopy.
  • a Raman spectrum or part thereof may be analyzed from the test stripe and optionally also from a control stripe of an hCG (human chorionic gonadotropin) lateral flow immunoassay pregnancy test kit, such as the AccuClear hCG pregnancy test kit (Inverness Medical Innovations, Inc.).
  • the Raman peaks at the test stripe (which is seen visually as a reddish stripe on the strip) are unique to the gold antibody conjugate, and the peak heights are directly proportional to the concentration of the captured gold complex.
  • the Raman peak heights provide a quantitative readout either as a direct reading of the analyte peak intensity or as a ratio to either the Control stripe or to an additional Raman feature on the strip.
  • concentration of an analyte in a sample may, thus, be determined.
  • the gold particles of the bead-antibody conjugate may enhance the Raman signal by the SERS effect. Whether or not the present invention is implemented for surface- enhanced or natural scattering intensity, it relies on the Raman signature of the analyte- binding complex (such as bead-antibody complex) without the addition of a special Raman label. Using control reagents a concentration calibration may be readily produced for comparison with the observed Raman peak intensities.
  • the size of the gold particles can be controlled when preparing the gold conjugates, a size may be chosen to optimize the Raman effect for quantification.
  • the present invention may also utilized beads of material other than gold.
  • beads made of other SERS-active materials such as silver, nickel, copper and/or cadmium may be used.
  • SERS-active metallic particles may, for example, be solid metallic particles or particles that are at least partially coated with a SERS- active metal. SERS techniques and materials are described in U.S. Patent Nos. 5,400,136 and 5,864,397, which are incorporated herein by reference in their entirety.
  • a Raman spectroscopy system of the invention may, for example, be used with an AccuClear hCG (human chorionic gonadotropin) test kit by measuring either the peak intensity directly (after a suitable calibration run) or by ratioing the Raman peak intensities at the T (test) and C (control) positions on the test kit after the liquid sample has been allowed to flow laterally though the kit.
  • AccuClear hCG human chorionic gonadotropin
  • test strip that may be a lateral flow test strip or non-lateral flow test strip
  • a test strip assay that is notched, has other physical elements and/or is marked to permit the operative alignment of the test strip with respect to the test strip receiving structure of the Raman spectroscopy system.
  • the test strip may be notched, have other physical elements and/or be marked to permit the operative alignment of the test strip with respect to the test strip receiving structure of the Raman spectroscopy system so that the test and/or control stripc(s) regions can be read.
  • the marking and/or notch(es) may be in register with the stripes or they can be offset from the stripes so long as the probe of the Raman reader unit is suitably positioned (coordinated) to read a/the strip when the mark or notch is correctly positioned.
  • a related embodiment provides a Raman reader that includes a test strip receiving member adapted to align, or allow the alignment of, the test stripes with illuminating and signal receiving elements of the Raman spectrometer based on a reference marking and/or notch(es) present on a lateral flow assay test strip.
  • a still further embodiment provides a system that includes the aforementioned test strip and a Raman reader unit that are adapted to be used together.
  • the lateral flow matrix may or may be at least partially housed in a casing.
  • the casing, rather than the actual strip material may include one or more reference markings, notches and/or other physical elements that permit the strip to be properly aligned in the reader for reading of the stripe(s).
  • FIG. 8 shows a lateral flow assay test strip 800, having a sample deposition area 832 and bilateral notches 833 formed at the axial position of a test stripe 840 and bilateral notches 835 formed at the position of a control stripe 850.
  • Arrow 870 illustrates the direction of lateral flow in the test strip.
  • FIG. 9 shows an example of the test strip of FIG. 8, positioned in a strip-positioning mechanism of a Raman reader apparatus.
  • Lateral protrusions 945 of the reader align with notches 833 of the test strip so that the strip clicks into position for reading of test stripe 840 (as currently shown) or control stripe 850 by the Raman probe 960 to illuminate the stripes and collect resulting Raman scattered light therefrom, along optical path 947.
  • the test strip 800 and/or protrusions 945 may have sufficient give or springiness so that the test strip can be clicked from one alignment position into another by an operator.
  • Guide rail "tongues" 980 of the reader unit overlay the edges of the test strip and collectively form a slot in which the test strip is guided. Arrow 990 shows the lateral direction in which the test strip can be moved forward and backwards.
  • the Raman reader unit prompts the operator to position the test strip at one or more test stripes and/or control stripes.
  • FIG. 10 shows a cross sectional view, along line 10 of FlG. 9, of the test strip positioned in the test strip-guiding rails of the reader unit.
  • Raman intensity peak height is directly proportional to the concentration of a scatterer.
  • the concentration of an analyte may, for example, be determined by multiplying the Raman intensity at a wavenumber or wavenumber band associated with the Raman scattering of the bead-bound analyte-binding element (which may be an antibody), or a complex or the analyte-binding element and analyte, at the test stripe for the analyte, with a proportionality constant, which may, for example, be determined in advance using control 06
  • a ratio of Raman signal readings from the test stripe and control stripes may, for example, be multiplied with a proportionality constant to obtain the desired analyte concentration. Using such a ratio advantageously corrects for potential calibration variabilities that may arise with the Raman reader unit.
  • Antibodies used as binding elements may be of any suitable form or type, may be produced by any method, and may be unprocessed or processed, for example proteolytically into FAb fragments.
  • Fluid and liquid samples that may be assayed according to the invention include, but are not limited to, blood, urine and saliva. Other body fluids that may be assayed include, for example, lymph and cerebrospinal fluid. Body fluids that are assayed may be unprocessed ("raw"), such as blood, or processed, such as plasma. Semi-fluids such as sputum or fecal matter may also be assayed.
  • Non-fluid or semi-fluid samples may be also fluidized or further fluidized for assay according to the invention.
  • the systems and methods of the invention advantageously may also be used to evaluate non-body fluid biological samples and non-biological samples using test substrates such as test strips. [00075] Multiple analyte analysis
  • the systems of the invention may advantageously be used to quantitatively and/or qualitatively analyze multiple analytes within a body fluid sample or other type of sample. This aspect of the invention is further described below with reference to body fluid samples, such as blood samples.
  • the device of the present invention investigates multiple analytes within a single sample during a single analysis it may be particularly advantageous to test analytes related to a single condition of interest. For example, when a patient arrives for a check-up, instead of running two diagnostic tests related to one condition, e.g., one for blood sugar, one for hemoglobin AIc, the present invention allows simultaneous testing. The result is a cost effective and almost immediate analysis.
  • one embodiment of the invention provides for the analysis of multiple analytes that are related to a single preselected condition or to the health status of a preselected organ or tissue system.
  • Other groups of analytes can include a blood chemistry profile.
  • urea blood urea nitrogen, "BUN"
  • BUN blood urea nitrogen
  • bilirubin e.g., total and/or direct bilirubin
  • phosphorous alkaline phosphatase
  • total protein albumin
  • globulin glucose, carbon dioxide, T-3, T-3 uptake
  • T-4 free thyroxine index
  • TSH free T-4
  • cholesterol triglycerides
  • HDL 5 LDL, VLDL iron, iron saturation, total iron binding and ferritin
  • assessments may be desirable to reveal the levels of beneficial and toxic chemical entities that commonly occur in humans as the result of lifestyle and toxic exposures. Such a test may be desirable for persons concerned with health hazards in their living or work space.
  • analytes may be chosen which focus on a certain preselected health condition. For example, testing for analytes related to cardiac health may be desirable during a regular check-up or as part of a heart health screening.
  • Such analytes may, for example, include two or more of cholesterol (e.g., total, LDL, and/or HDL), triglycerides, C-reactive protein (CRP), brain natriuretic peptide (BNP), troponin, myeglobin and homocysteine.
  • analytes could include two or more of marijuana (including but not limited to active substance THC), amphetamines, methamphetamines, barbiturates, benzodiazepines, cocaine, 3,4- methylenedioxymethylamphetamine (MDMA), opioids (such as but not limited to morphine, heroin and synthetic opioids), D-lysergic acid N,N-diethylamide (LSD) and 1-(1- phenylcyclohexyl)piperidine (PCP).
  • marijuana including but not limited to active substance THC
  • amphetamines methamphetamines, barbiturates
  • benzodiazepines cocaine, 3,4- methylenedioxymethylamphetamine (MDMA)
  • opioids such as but not limited to morphine, heroin and synthetic opioids
  • LSD D-lysergic acid N,N-diethylamide
  • PCP 1-(1- phenylcyclohexyl)piperidine
  • the Raman spectroscopy systems of the invention may be used to monitor pharmacotherapy in human or animal subjects.
  • the quantification of a preselected drug and/or its metabolites in a sample obtained from a patient may be used to determine whether the active species of the drug is/are present at a desired concentration in the patient, and if not, the cause of the problem.
  • the activity of many therapeutic drugs is at least partially dependent on their metabolism to one or more active forms within the body. For example, buproprion (ZybanTM, WellbutrinTM) is extensively metabolized into three active metabolites.
  • ACE antihypertensive angiotensin I-converting enzyme
  • Determining the relative concentration of parent drug to active metabolite(s) in a patient provides an indication of whether drug metabolism is normal and/or whether an abnormality in metabolism that interferes with the pharmacotherapy may be present, for example due to a metabolic insufficiency in the patient. Determining the concentration(s) of parent drug and/or metabolites thereof in a patient can provide information as to whether there is a problem in drug absorption/administration or a patient's compliance therewith, if the desired level of drug or metabolites is not achieved.
  • Therapeutic drugs that may be monitored and/or evaluated for metabolism according to the invention include, but are not limited to: antiarrhythmics such as digoxin and quinidine; antibiotics such as vancomycin, gentamicin, tobramycin, amikacin and netilmycin; anti-epileptics such as phenytoin, phenobarbital, carbamazepine, and ethosuximide; anti-asthmatics such as theophylline, and anti-rejection drugs such as cyclosporine.
  • antiarrhythmics such as digoxin and quinidine
  • antibiotics such as vancomycin, gentamicin, tobramycin, amikacin and netilmycin
  • anti-epileptics such as phenytoin, phenobarbital, carbamazepine, and ethosuximide
  • anti-asthmatics such as theophylline
  • anti-rejection drugs such as cyclosporine.
  • results can be displayed quantitatively (120 mg/dl) or relatively (Normal). With other analytes, it may be desirable to display results indicating only the presence (or absence) of an analyte (e.g., the presence of poisons).

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Abstract

L'invention concerne des appareils de diagnostic qui sont adaptés de manière avantageuse pour l'analyse spectroscopique de Raman d'échantillons de fluides, tels que des échantillons de fluides biologiques, déposés sur des substrats de bandelettes réactives. Les bandelettes réactives peuvent être comprises sur une surface de spectroscopie de Raman améliorée en surface (SERS) pour le dépôt et l'analyse d'un échantillon et/ou peuvent être des bandelettes réactives de type test d'immunochromatographie sur membrane.
PCT/US2007/002106 2006-01-27 2007-01-26 systèmes de bandelettes réactives spectroscopiques de Raman WO2007089551A2 (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US11/340,712 2006-01-27
US11/340,712 US7651851B2 (en) 2005-01-27 2006-01-27 Handheld Raman body fluid analyzer
US76569906P 2006-02-06 2006-02-06
US60/765,699 2006-02-06
US83725606P 2006-08-11 2006-08-11
US60/837,256 2006-08-11

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US8467053B2 (en) 2008-06-03 2013-06-18 The Research Foundation Of State University Of New York Identification of body fluids using raman spectroscopy
TWI827555B (zh) * 2017-10-16 2024-01-01 美商再生元醫藥公司 灌注生物反應器及相關使用方法

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Publication number Priority date Publication date Assignee Title
US8467053B2 (en) 2008-06-03 2013-06-18 The Research Foundation Of State University Of New York Identification of body fluids using raman spectroscopy
TWI827555B (zh) * 2017-10-16 2024-01-01 美商再生元醫藥公司 灌注生物反應器及相關使用方法
US11965153B2 (en) 2017-10-16 2024-04-23 Regeneron Pharmaceuticals, Inc. Perfusion bioreactor and related methods of use

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