WO2007084499A2

WO2007084499A2 - Disease control in shrimp

Info

Publication number: WO2007084499A2
Application number: PCT/US2007/001125
Authority: WO
Inventors: Arun K. Dhar
Original assignee: San Diego State University
Priority date: 2006-01-13
Filing date: 2007-01-16
Publication date: 2007-07-26
Also published as: WO2007084499A8

Abstract

The invention pertains to the composition and methods of use of differentially expressed genes in crustaceans, particularly that of shrimp. Genes that have special significance to the ability of shrimp to combat viral disease are described and their selection, amplification, and application for useful purposes. Compositions, including nucleic acid sequences and the polypeptides encoded by them, are described that are useful in feeds, therapeutics, diagnostics, and research. Methods to reduce the effect of viral infection, especially of the white spot syndrome virus, are also described. The uses of the described genes to monitor and diagnose viral disease are also disclosed.

Description

DISEASE CONTROL IN SHRIMP

Technical Field

[0001] The invention pertains to the identification, monitoring, and treatment of infection in crustaceans, particularly Penaeus sp. (shrimp). The compositions and methods using nucleic acids and polypeptides of the invention, e.g., sequences identified by differentially expression in non-infected and infected crustaceans, in therapeutics, diagnostics, and screening reagents, particularly for viral infection in Penaeus sp..

Background of the Invention [0002] During the past decade, shrimp (Penaeus sp.) farming has evolved from subsistence level farming to a major worldwide industry providing jobs to millions of people. Currently, shrimp farming remains a major source for economic development in the poor coastal areas of many countries in the Asia and the Americas (Rosenberry, 2002). As shrimp farming evolves, it is faces many challenges. Among these challenges, viral diseases are of major concern to shrimp farmers. More than 20 viruses have been reported to infect shrimp (Lightner 1996), and the list is growing. Many of these viruses have already caused serious disease in cultured and wild penaeid shrimp resulting in significant economic losses to commercial shrimp farmers. At present, white spot disease of shrimp, caused by the white spot syndrome virus (WSSV), is considered to be the most important viral disease of cultured shrimp worldwide (Office International de Epizooties, 2002). Since the initial report of WSSV in East Asia during 1992 to 1993 (Inouye et al. 1994), WSSV has spread to much of Asia and the Americas causing catastrophic losses to shrimp farmers (Krishna et al. 1997; Jory and Dixon 1999). The cumulative loss due to WSSV in the Asia since 1992 is estimated to be $4-6 billion. The losses due to WSSV in the Americas have been estimated to $1-2 billion. [0003] WSSV virions are ellipsoid to bacilliform in shape, enveloped with a tail- like appendage at one end of the particle. The genome of WSSV contains a circular double-stranded DNA of -300 kb in length (van Hulten et al. 2001; Yang et al. 2001). Although the WSSV has a morphological similarity with baculovirus, sequence analysis revealed that WSSV shares very little similarity with any known viruses (van Hulten et al, 2001 ; Yang et al , 2001). As a result, WSSV has been placed in a new family, the Nimaviridae, and a new genus, Whispovirus. [0004] WSSV infects all commercially important species of penaeid shrimp and a number of other crustaceans, including crabs and crayfish (Flegel 1997). Since the initial report of WSSV in East Asia during 1992 to 1993 (Inouye et al 1994), a number of WSSV-encoded genes, such as the capsid genes (van Hulten et al 2000a; van Hulten et al. 2000b; Zhang et al 2001 ; Chen et al 2002; Marks et al 2003), a ribonucleotide reductase gene (Tsai et al. 2000a), and the thymidine kinases (Tsai et al 2000b) have been studied in detail. In addition, a highly sensitive detection method based on real- time PCR has been developed for detecting and quantifying WSSV (Dhar et al. 2001). However, insight into immune genes in shrimp involved in WSSV pathogenesis is limited and only just beginning to emerge. For example, Dhar and colleagues cloned and sequenced a lipopolysaccharide and β-l,3-ghican binding protein (LGBP) gene from Penaeus stylirostris shrimp that shows up-regulation during WSSV infection (Roux et al. , 2002). LGBP, a pattern recognition protein (PRP), is known to elicit the expression of prophenoloxidase (proPO) during bacterial and fungal infection (Soderhall and Cerenius, 1998). Although LGBP gene expression is upregulated in WSSV-infected animals, proPO gene expression is downregulated as the WSSV infection progresses, suggesting that WSSV infection regulates the activation and / or activity of the prophenoloxidase cascade in a novel way (Roux et al, 2002). A syntenin-like protein (TE8) with a post-synaptic density protein (PDZ) domain has been isolated from Penaeus monodon shrimp and was upregulation during WSSV- infection (Bangrak et al, 2002). It has been suggested that the shrimp syntenin-like protein may function as an adapter that couples the PDZ-binding protein to cell-to-cell signal transduction during WSSV pathogenesis (Bangrak et al , 2002). Antiviral substances capable of binding to a variety of DNA and RNA viruses (Sindbis virus, vaccinia virus, vesicular somatitis virus, mengo virus, banzi virus and poliomyelitis virus) have been isolated from shrimp {Penaeus setiferus), although the genes representing these proteins have not yet been cloned (Pan et al, 2000). These antiviral proteins probably represent a component of the innate immune response in shrimp (Pan et al, 2000). All the above examples of the characterization of immune genes and associated proteins in shrimp revealed the candidate gene approaches used by different researchers. Although such approaches may provide in depth information on a particular gene, they do not provide a holistic view of gene expression during viral or microbial pathogenesis. [0005] To mitigate these challenges and to promote sustainable shrimp farming, there is an urgent need to develop therapeutics against white spot disease and viral diseases in shrimp in general. One of the limitations in developing therapeutics against viral diseases of shrimp is the lack of information on the cellular genes that might be involved in WSSV pathogenesis. This invention addresses this need by identifying cellular genes that play a critical role in viral, specifically WSSV, pathogenesis in shrimp, and viral pathogenesis in invertebrates in general. Several immune genes in shrimp have been isolated that showed differential expression between healthy and WSSV-infected shrimp. These genes could be used as potential targets for developing therapeutics against white spot disease and other viral, bacterial, and fungal diseases in shrimp.

Summary

[0006] This invention has identified cellular genes that play a critical role in viral, specifically white spot syndrome virus (WSSV), pathogenesis in Penaeus sp. (shrimp), and viral pathogenesis in invertebrates in general. Several immune genes in shrimp have been isolated that showed differential expression between healthy and WSSV- infected shrimp. The nucleic acids comprising these genes, or fragments thereof, can used as potential targets for developing therapeutics against white spot disease and other viral, bacterial, and fungal diseases in shrimp. Accordingly, the invention provides methods for screening for compounds for treating or diagnosing WSSV using the nucleic acids of the invention or the polypeptides they encode.

[0007] The invention provides compositions comprising, or consisting of, sets of differentially expressed genes selected by their differential expression on viral infection as compared to healthy Penaeus sp. (shrimp) expression profiles. In one aspect, the viral infection is white spot syndrome virus, Taura syndrome virus, infectious hypodermal and hematopoietic virus, yellowhead virus or baculovirus penae.

[0008] In one aspect, the genes are selected from expressed sequence tag libraries designed by differential selection of genes that are up- or down-regulated during viral infection. In one aspect, the viral infection is white spot syndrome virus. The genes can the genes described herein, e.g., the nucleic acids as set forth in Table 2 and the appendices. In one aspect, the viral infection is selected from white spot syndrome virus, Tarua syndrome virus, infectious hypodermal and hematopoietic virus, yellowhead virus and baculovirus penae. The genes can be selected from the shrimp expressed sequence tag differential library used for the treatment of disease. The disease can be selected from white spot syndrome virus, Tarua syndrome virus, infectious hypodermal and hematopoietic virus, yellowhead virus and baculovirus penae. The differential library can be produced from shrimp infected with WSSV, or, from shrimp infected with viruses selected from white spot syndrome virus, Tarua syndrome virus, infectious hypodermal and hematopoietic virus, yellowhead virus and baculovirus penae.

[0009] The invention also provides methods of determining the metabolic or disease state of shrimp using the compositions described herein, including the polypeptides of the invention and the nucleic acids of the invention.

[0010] The invention provides isolated or recombinant nucleic acids having at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more sequence identity, when compared and aligned for maximum correspondence, as measured using one any known sequence comparison algorithm, as discussed in detail below, or by visual inspection, to an exemplary nucleic acid of the invention, which include all nucleic acids sequences described herein, e.g., as set forth in Appendix A. [00H ] The invention provides isolated or recombinant polypeptides having at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more sequence identity, when compared and aligned for maximum correspondence, as measured using one any known sequence comparison algorithm, as discussed in detail below, or by visual inspection, to an exemplary polypeptides of the invention, which include all polypeptides described herein, and include all polypeptides encoded by nucleic acids of the invention, which include all nucleic acids sequences described herein, e.g., as set forth in Appendix A. [0012] In alternative aspects, the invention provides nucleic acid and polypeptide sequences having substantial identity to an exemplary sequence of the invention over a region of at least about 10, 20, 30, 40, 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000 or more residues. [0013] It is an object of the invention to provide a number of expressed sequence tags (ESTs) isolated from shrimp tissue that specifically correspond to the onset of viral disease.

[0014] The invention provides nucleic acids, e.g., probes or expressed sequence tags (ESTs), some isolated from Penaeid shrimp tissue; that specifically correspond to the onset of viral disease. In one aspect, these nucleic acids, e.g., probes or expressed sequence tags comprise subsequences of sequences of the invention, including the exemplary sequences set forth herein, e.g., as set forth in Appendix A. The invention provides nucleic acids, e.g., probes or expressed sequence tags (ESTs), comprising subsequences of sequences of the invention, including the exemplary sequences set forth herein, the specifically correspond to the onset of viral disease. In one aspect, these nucleic acids have been isolated from shrimp (Penaeus vannamei) tissue.

[0015] The invention provides compositions for use in protecting Penaeus sp. (shrimp) from viral infection and methods of protecting Penaeus sp. (shrimp) from viral infection using recombinant or isolated protein or nucleic acids comprising sequences of the invention, including the exemplary sequences set forth herein, e.g., as set forth in Appendix A; which in various aspects are derived from the full-length gene or recombinant truncated protein or nucleic acids derived from the functional domain of the gene identified using the EST differential libraries of the instant invention. [0016] The invention provides compositions and methods for use in protecting

Penaeus sp. (shrimp) from WSSV infection or other viral infection using recombinant or isolated protein or nucleic acids comprising sequences of the invention, including the exemplary sequences set forth herein, e.g., as set forth in Appendix A; which in various aspects are derived from the full-length gene or recombinant truncated protein or nucleic acids derived from the functional domain of the gene.

[0017] The invention provides feeds and/or feed supplements which comprise recombinant or isolated protein or nucleic acids comprising sequences of the invention, including the exemplary sequences set forth herein, e.g., as set forth in Appendix A; which in various aspects incorporate recombinant or isolated protein or nucleic acids identified using the EST differential libraries of the instant invention and providing same to the animal to inhibit the deleterious effects of the virus on the host. [0018] The invention provides compositions and methods for treatments comprising use of recombinant or isolated protein or nucleic acids comprising sequences of the invention, including the exemplary sequences set forth herein, e.g., as set forth in Appendix A; which in various aspects incorporate recombinant or isolated protein or nucleic acids identified using the WSSV infected and healthy EST differential libraries of the instant invention and providing it to the animal to inhibit or suppress the deleterious effects of the WSSV on the host. [0019] The invention provides therapeutics, a feed, or feed supplement that incorporates recombinant or isolated protein or nucleic acids identified using the WSSV infected and healthy EST differential libraries of the instant invention and providing it to the animal to inhibit or suppress the deleterious effect of the WSSV on the host. [0020] The invention provides methods of treatment for white spot viral disease in

Penaeus sp. (shrimp) based on the genes identified through differential expression libraries, including protein or nucleic acids comprising sequences of the invention, including the exemplary sequences set forth herein, e.g., as set forth in Appendix A. [0021] It is an object of the invention to provide a method of treatment wherein the method comprises the steps including the production of recombinant or isolated protein using bacterial, yeast, plant, and/or algal expression systems, then mixing the recombinant or isolated protein with feed and delivering the recombinant or isolated protein in sufficient quantity to prevent action of the virus on Penaeus sp. (shrimp). This approach can also be applied to diseases in Penaeus sp. (shrimp) caused other viruses, bacteria, fungi, as well as in other aquaculture species including fish and shellfish suffering from fungal, bacterial, and viral diseases. In aquatic invertebrate species, like Penaeus sp. (shrimp), with a primitive immune system (i.e., one that is not antibody driven) the invention provides a method for treatment of both acute and chronic diseases via delivery of therapeutic recombinant or isolated protein(s) and/or nucleic acids.

[0022] It is an objective of this invention to use sequences of the invention, including the exemplary sequences set forth herein, e.g., as set forth in Appendix A, including ESTs differentially expressed on viral infection, as a biopesticide.

[0023] It is an object of the invention to provide a method using sequences of the invention, including the exemplary sequences set forth herein, e.g., as set forth in Appendix A, including the differentially expressed genes from the EST differential library, as a diagnostic tool to evaluate Penaeus sp. (shrimp) both in aquaculture and in food processing. [0024] It is an object of the invention to provide a useful resource for functional genomics study in Penaeus sp. (shrimp) using microarray analysis and single nucleotide polymorphisms (SNPs) and a resource for microsatellite sequence useful for developing Penaeus sp. (shrimp) genome map. Thus, the invention provides arrays comprising sequences of the invention, including the exemplary sequences set forth herein, e.g., as set forth in Appendix A.

[0025] It is an object of the invention to provide a feed or feed supplement comprising sequences of the invention, including the exemplary sequences set forth herein, e.g., as set forth in Appendix A; including recombinant or isolated protein(s) or nucleic acid(s), derived from the expressed sequence tags from healthy and WSSV- infected libraries, that can inhibit or suppress the deleterious effect of other Penaeus sp. (shrimp) viruses including the Taura syndrome virus, yellow head virus, and infectious hypodermal and hematopoietic necrosis virus and WSSV (Lightner and Redman, 1998). [0026] It is an object of the invention to provide a method of treatment for bacterial diseases of Penaeus sp. (shrimp) and other aquaculture species using sequences of the invention, including the exemplary sequences set forth herein, e.g., as set forth in Appendix A, including sequences based on the genes identified through Penaeus sp. (shrimp) EST analysis. [0027] Therefore, the invention provides both compositions that can be used for prevention of disease and methods of prevention of disease, and diagnostic tools for evaluation of the health of the animals affected by disease.

Brief Description of the Drawings

[0028] FIG. 1 is a graphical representation of the function classes of genes isolated from hepatopancreas cDNA libraries of healthy and WSSV infected shrimp (P. vannamei) that showed similarities with the GenBank database entries;

[0029] FIG. 2 is a graphical representation of the differential expression of expressed sequence tags (ESTs) in white spot syndrome virus (WSSV) infected shrimp (Penaeus vannamei) compared to healthy shrimp (P. vannamei). Bars above the X-axis indicate up-regulation, and bar below the X-axis indicates down-regulation of the gene in WSSV-infected compared to healthy shrimp; and

[0030] FIG. 3 is a graphical representation of the quantification of differently expressed immune genes in WSSV infected shrimp (P. vannemef) by SYBR Green real-time PCR. Bars above the axis represent up-regulated genes and bars below the axis represent down-regulated genes. 1) gamma interferon inducible lysosomal thiol reductase (GILT); 2) Interleukin enhancer-binding factor 3; 3) tetraspanin-2; 4) NF- kappaβ essential modulator; 5) Chitinase; 6) prophenoloxidase-activating proteinase 2; 7) p-selectin; 8) T-cell activation protein; 9) O.-sialoglycoprotein endopeptidase; 10) ubiquitin; 11) anti-lipopolysaccharide factor.

Detailed Description of the Invention

[0031] Provided herein is a nucleic acid sequence comprising a nucleic acid sequence of at least 65% identity to a nucleic acid sequence as set forth in as set forth in Appendix A.

[0032] . In one embodiment, the nucleic acid sequence has at least 95% identity. In another embodiment, the nucleic acid sequence has 100% identity. The invention also provides the polypeptide encoded by the nucleic acid sequences disclosed herein. Provided herein is a vector comprising the nucleic acid sequence provided herein, and a cell comprising said vector.

[0033] Further provided herein is a feed or feed supplement comprising sequences of the invention, including the exemplary sequences set forth herein, e.g., as set forth in Appendix A; including recombinant or isolated proteins or biologically active fragments thereof encoded by a nucleic acid sequence of the invention, e.g., having at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%,

63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%,

^' 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%,

93%, 94%, 95%, 96%, 97%, 98%, 99%, or more sequence identity to a shrimp gene or fragment thereof, wherein said shrimp gene is up-regulated during viral infection. In one embodiment, the shrimp gene is selected from a group of shrimp genes consisting of tetraspanin-2, P-selectin precursor, T-cell activation protein, anti-lipopolysaccharide factor, and heat shock protein STIl . In a specific embodiment, the shrimp gene is in clone PvWl 1A5, clone PvW4E03, clone PvW5G04, clone PvHBl 1, or clone PvWl 0D06. In some embodiment, the protein or biologically active fragment thereof is encoded by a nucleic acid listed in Appendix A. The protein can provided within a cell or provided as a disrupted cell.

[0034] Provided herein is a feed or feed supplement comprising a nucleic acid sequence of at least 65% sequence identity to a shrimp gene or biologically active portion thereof in a set of genes up-regulated by infection with a virus. The nucleic acid sequence can be a nucleic acid sequence listed in Appendix A. In one embodiment, the shrimp gene is selected from a group of shrimp genes consisting of tetraspanin-2, P-selectin precursor, T-cell activation protein, anti-lipopoly saccharide factor, and heat shock protein STIl . In a specific embodiment, the shrimp gene is contained in clone PvWl 1 A5, clone PvW4E03, clone PvW5G04, clone PvHBl 1, or clone PvWl 0D06. The nucleic acid sequence can be contained or provided within a cell or provided as a disrupted cell. In some embodiments, the nucleic acid sequence is produced synthetically. [0035] Also provided herein is a feed or feed supplement comprising a recombinant or isolated protein or biologically active fragment thereof encoded by a nucleic acid sequence with at least 65% or sequence identity to a nucleic acid sequence found in the set of shrimp genes down-regulated with viral infection. In one embodiment, the protein is gamma-interferon inducible lysosomal thiol reductase precursor, interleukin enhancer-binding factor 3₅ NF-kappaB essential modulator, chitinase, prophenoloxidase-activating proteinase 2, O-sialoglycoprotein endopeptidase, ubiquitin, or lysozyme. In a specific embodiment, the protein is encoded by a nucleic acid sequence selected from the group of shrimp nucleic acid sequence contained in clonePvHlA02, clone PvW8B06, clone PvW8E09, clone PvW9El l, clone PvW4F07, clone PvHC06, clone PvW04C06, and clone PvWl 0F4. In some embodiments, the protein or biologically active fragment thereof is encoded by a nucleic acid sequence listed in Appendix A. The protein can be provided within a cell or provided as a disrupted cell.

[0036] Provided herein is a feed or feed supplement comprising a nucleic acid sequence with at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more sequence identity to a nucleic acid of the invention, e.g., a shrimp gene or biologically active fragment thereof, wherein said nucleic acid sequence encodes a shrimp gene down-regulated at or during viral infection. The nucleic acid sequence can be one listed in Appendix A. In some embodiments, the shrimp gene is gamma-interferon inducible lysosomal thiol reductase precursor, interleukin enhancer-binding factor 3, NF-kappaB essential modulator, chitinase, prophenoloxidase-activating proteinase 2, O-sialoglycoprotein endopeptidase, ubiquitin, and lysozyme. In a specific embodiment, the shrimp nucleic acid sequence is contained in clonePvHl A02, clone PvW8B06, clone PvW8E09, clone PvW9El I₅ clone PvW4F07, clone PvHC06, clone PvW04C06, and clone PvWl 0F4. The nucleic acid sequence can be provided within a cell or provided as a disrupted cell. In some embodiments, the nucleic acid sequence is produced synthetically.

[0037] Further provided herein is a therapeutic comprising a recombinant or isolated protein or biologically active portion thereof having at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more sequence identity to a nucleic acid of the invention, e.g., including a shrimp protein encoded by a shrimp gene up-regulated during viral infection. In one embodiment, the shrimp gene is a nucleic acid sequence listed in Appendix A. The shrimp gene can be tetraspanin-2, P-selectin precursor, T-cell activation protein, anti-lipopolysaccharide factor, and heat shock protein STI 1. In a specific embodiment, the shrimp gene is contained in clone PvWl 1 A5, clone PvW4E03, clone PvW5G04, clone PvHBl 1, and clone PvWl 0D06. The protein or biologically active portion thereof can be delivered orally, by immersion, by injection, or any suitable method. [0038] Provided herein is a therapeutic comprising a nucleic acid sequence of at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more sequence identity to a nucleic acid of the invention, e.g., to a shrimp gene or biologically active fragment thereof that is up- regulated by infection with a virus. The shrimp gene can be selected from those listed in Appendix A. In one embodiment, the shrimp gene is tetraspanin-2, P-selectin precursor, T-cell activation protein, anti-lipopolysaccharide factor, and heat shock protein STIl. In a specific embodiment, the shrimp gene is contained in clone PvWl 1A5, clone PvW4E03, clone PvW5G04, clone PvHBl 1, and clone PvWl 0D06. The nucleic acid sequence can be delivered orally, by immersion, by injection, or any suitable method that permits expression.

[0039] Further provided is a therapeutic comprising a recombinant or isolated protein or biologically active fragment thereof encoded by a nucleic acid sequence with at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more sequence identity to a nucleic acid of the invention, e.g., to a shrimp protein down-regulated by infection with a virus. The protein or biologically active fragement thereof can be encoded by a nucleic acid sequence listed in Appendix A. In one embodiment, the protein or biologically active fragment thereof is gamma-interferon inducible lysosomal thiol reductase precursor, interleukin enhancer-binding factor 3, NF-kappaB essential modulator, chitinase, prophenoloxidase-activating proteinase 2, O-sialoglycoprotein endopeptidase, ubiquitin, or lysozyme. In a specific embodiment, the protein or biologically active fragment thereof is contained in clonePvHlA02, clone PvW8B06, clone PvW8E09, clone PvW9El 1, clone PvW4F07, clone PvHC06, clone PvW04C06, or clone PvWl 0F4. The protein or biologically active portion thereof can be delivered orally, by immersion, by injection, or any suitable method.

[0040] Also provided herein is a therapeutic comprising a nucleic acid sequence of at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more sequence identity to a nucleic acid of the invention, e.g., including a shrimp gene or faction thereof down-regulated by infection with a virus. The nucleic acid sequence can be one listed in Appendix A. In one embodiment, the nucleic acid sequence is gamma-interferon inducible lysosomal thiol reductase precursor, interleukin enhancer-binding factor 3, NF-kappaB essential modulator, chitinase, prophenoloxidase-activating proteinase 2, O-sialoglycoprotein endopeptidase, ubiquitin, and lysozyme. In a specific embodiment, the nucleic acid sequence is contained in clone PvHl A02, clone PvW8B06, clone PvW8E09, clone PvW9El 1, clone PvW4F07, clone PvHC06, clone PvW04C06, or clone PvWl 0F4. The nucleic acid sequence can be delivered orally, by immersion, by injection, or any suitable method that permits expression.

[0041] Provided in a screening method for identifying a substrate of a protein that is up-regulated following infection by a pathogen, comprising: (a) providing a polypeptide of the invention; or a polypeptide encoded by a nucleic acid of the invention; (b) providing a test substrate; and (c) contacting the polypeptide of step (a) with the test substrate of step (b) and detecting a decrease in the amount of substrate or an increase in the amount of reaction product, wherein a decrease in the amount of the substrate or an increase in the amount of a reaction product identifies the test substrate as the polypeptide substrate. The protein can be encoded by a nucleic acid sequence selected from Appendix A. In one embodiment, the protein is tetraspanin-2, P-selectin precursor, T-cell activation protein, anti-lipopolysaccharide factor, or heat shock protein STIl. In a specific embodiment, the protein is encoded by the nucleic acid seqeunce contained in clone PvWl 1 A5, clone PvW4E03, clone PvW5G04, clone PvHBl 1, or clone PvWl 0D06. [0042] Further provided herein is a screening method cfor identifying a substrate of a protein that is down-regulated following infection by a pathogen, comprising: (a) providing a polypeptide of the invention; or a polypeptide encoded by a nucleic acid of the invention; (b) providing a test substrate; and (c) contacting the polypeptide of step (a) with the test substrate of step (b) and detecting a decrease in the amount of substrate or an increase in the amount of reaction product, wherein a decrease in the amount of the substrate or an increase in the amount of a reaction product identifies the test substrate as the polypeptide substrate. The protein can be encoded by a nucleic acid sequenced selected from Appendix A. In one embodiment, the protein is gamma- interferon inducible lysosomal thiol reductase precursor, interleukin enhancer-binding factor 3, NF-kappaB essential modulator, chitinase, prophenoloxidase-activating proteinase 2, O-sialoglycoprotein endopeptidase, ubiquitin, or lysozyme. In a specific embodiment, the protein is encoded by the nucleic acid sequence contained in clonePvHlA02, clone PvW8B06, clone PvW8E09, clone PvW9El 1, clone PvW4F07, clone PvHC06, clone PvW04C06, or clone PvWl 0F4. [0043] Also provided hererin is a diagnostic kit for evaluation of infection in crustaceans in aquaculture and food processing comprising at least one differentially expressed gene as a nucleic acid or recombinant polypeptide, wherein the gene can be used to identify a crustacean as being infected or non-infected with the pathogen of interest; and optionally instructions for use of the kit. The gene can be one selected from Appendix A.

[0044] Provided herein is a method for treating or preventing infection by a pathogen in an aquatic animal, e.g., shrimp, comprising providing at least one differentially expressed gene as a nucleic acid or recombinant polypeptide, wherein the gene is up-regulated with infection by the pathogen. The gene can be one selected from Appendix A. In one embodiment, the gene is tetraspanin-2, P-selectin precursor, T-cell activation protein, aήti-lipopoly saccharide factor, or heat shock protein STIl. In a specific embodiment, the gene is contained in clone PvWl 1 A5, clone PvW4E03, clone PvW5G04, clone PvHBl 1, or clone PvWlODOo. [0045] Further provided herein is a method for treating or preventing infection by a pathogen in an aquatic animal, e.g., shrimp, comprising providing at least one differentially expressed gene as a nucleic acid or recombinant polypeptide, wherein the gene is down-regulated with infection by the pathogen. The gene can be one selected from Appendix A. In one embodiment, the gene is gamma-interferon inducible lysosomal thiol reductase precursor, interleukin enhancer-binding factor 3, NF-kappaB essential modulator, chitinase, prophenoloxidase-activating proteinase 2, O- sialoglycoprotein endopeptidase, ubiquitin, or lysozyme: In a specific embodiment, the gene is the nucleic acid sequence contained clonePvHlA02, clone PvW8B06, clone PvW8E09, clone PvW9El 1, clone PvW4F07, clone PvHC06, clone PvW04C06, or clone PvWl 0F4. The aquatic animal is a crustacean, preferably a shrimp.

[0046] Also provided herein is an inhibitory RNA molecule comprising a nucleic acid sequence comprising a nucleic acid sequence or fragment thereof, or complementary sequence, of the nucleic acid sequence listed in Appendix A. In one embodiment, the inhibitory RNA molecule can be used in a method of preventing or treating infection of an aquatic animal comprising administering the inhibitory RNA molecule to the animal. The inhibitory RNA molecule can be used in a feed or feed supplement. The inhibitory RNA molecule can be provided in a cell or as a disrupted cell. The cell can be a bacterial, yeast, insect, fish, crustacean, or mammalian cell. [0047] Provided herein is a method of identifying shrimp lines that are resistant to viral, bacterial, or fungal diseases, comprising identifying at least one differentially expressed gene as a nucleic acid or protein, wherein the gene comprises a nucleic acid sequence provided in Appendix A, or a subsequence thereof.

[0048] Further provided herein is a method of screening for a therapeutic that modulates infectious disease in an aquatic animal, comprising identifying a compound which modulates the expression of the gene as a nucleic acid sequence or protein in an infected animal, wherein the gene comprises a nucleic acid sequence listed in Appendix A, or a subsequence thereof, whereby the compound is identified as a modulator of infectious disease when said modulation results in the ameriolation or prevention of one or more symptoms caused by the infection. [0049] Also provided herein is a microsatellite marker comprising at least one nucleic acid sequence listed in Appendix A, or a subsequence thereof. Provided herein is a method of developing microsatellite markers employing at least one nucleic acid sequence listed in Appendix A, or a subsequence thereof. [0050] Provided herein is a microarry comprising at least one nucleic acid sequence or biologically active fragment thereof listed in Appendix A, or a subsequence thereof.

[0051] Further provided is a biopesticide comprising at least one nucleic acid sequence or biologically active fragment thereof listed in Appendix A, or a subsequence thereof. [0052] Provided is a method for developing a biopesticide employing at least one nucleic acid sequence or biologically active fragment thereof listed in Appendix A, or a subsequence thereof.

[0053] Further provided is a transgenic plant expressing a protein or biologically active fragment thereof encoded by a nucleic acid sequence disclosed in Appendix A, or a subsequence thereof.

Definitions

[0054] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one of ordinary skill in the art to which this invention belongs. All patents, published patent applications and other publications and sequences from GenBank and other databases referred to herein are incorporated by reference in their entirety. If a definition set forth in this section is contrary to or otherwise inconsistent with a definition set forth in patents, published patent applications and other publications and sequences from GenBank and other data bases that are herein incorporated by reference, the definition set forth in this section prevails over the definition that is incorporated herein by reference.

[0055] As used herein, "a" or "an" means "at least one" or "one or more." [0056] As used herein, the term "aquaculture" refers to the cultivation of aquatic organisms under controlled conditions. An "aquatic organism" is an organism grown in water, either fresh- or saltwater. Aquatic organisms, include, but are not limited to, fish, e.g., bass, striped bass, tilapia, catfish, sea bream, rainbow trout, zebrafish, red drum, and carp; crustaceans, e.g., penaeid shrimp, brine shrimp, freshwater shrimp, and Artemia; and rotifers. [0057] A "coding sequence" is an in-frame sequence of codons that (in view of the genetic code) correspond to or encode a protein or peptide sequence. Two coding sequences show similarity or homology to each other if the sequences or their complementary sequences encode the same or similar amino acids. [0058] An "EST" or "expressed sequence tag" is a piece of nucleic acid that is found under specific conditions and is derived from a cDNA library. A unique DNA sequence derived from a cDNA library (therefore from a sequence which has been transcribed in some tissue or at some stage of development). The EST can be mapped, by a combination of genetic mapping procedures, to a unique locus in the genome and serves to identify that genetic locus.

[0059] A "feed" refers to a preparation providing nutritional value to an aquatic animals including, but not limited to fish, shrimp, lobsters, crawfish, mollusks, sponges, and jellyfish.

[0060] A "feed additive" is any substance added to feed, regardless of nutritional or therapeutic value.

[0061] As used herein, the term "shrimp" refers to any of the group of crustaceans that are commonly cultured for aquaculture or captured in the wild fisheries. The term "shrimp" includes shrimp eggs, shrimp larvae, shrimp post-larvae and adult shrimp. The term "shrimp" and "prawn" will be used interchangeably throughout the specification. Shrimp an be, but are not limited to Penaeus shrimp and include the species Penaeus vannamel, Penaeus chinensis, Penaeus monodon, Penaeus stylirostris, Penaeus japonicus, Penaeus penicillatus, Penaeus merguiensis, Penaeus indicus, Penaeus subtϊlis, Penaeus paulensis, Penaeus setiferus, Penaeus brasiliensis, Penaeus duorarum, Penaeus occidentalis, Penaeus schmitti, Penaeus californiensis, Penaeus semisulcatus, Penaeus latisulcatus, Metapenaeus monoceros, Metapenaeus dobsoni, Metapenaeus affinis, and Metapenaeus brivicornis; and Litopenaeid shrimp (such as Litopenaeus vannamei, L. setiferus).

[0062] "Contig" refers to a continuous sequence of DNA that has been assembled from overlapping nucleotide sequence of cDNA clones. [0063] "Singleton" refers to a single EST sequence representing a particular gene.

[0064] "Unigene set" refers to non-redundent set of gene-oriented clusters. [0065] The term "antibody" includes a peptide or polypeptide derived from, modeled after or substantially encoded by an immunoglobulin gene or immunoglobulin genes, or fragments thereof, capable of specifically binding an antigen or epitope, see, e.g. FUNDAMENTAL IMMUNOLOGY, Third Edition, W.E. Paxil, ed., Raven Press, N. Y. (1993); Wilson (1994) J. Immunol. Methods 175:267-273; Yarmush (1992) J. Biochem. Biophys. Methods 25:85-97. The term antibody includes antigen-binding portions, i.e., "antigen binding sites," (e.g., fragments, subsequences, complementarity determining regions (CDRs)) that retain capacity to bind antigen, including (i) a Fab fragment, a monovalent fragment consisting of the VL, VH, CL and CHl domains; (ii) a F(ab')2 fragment, a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region; (iii) a Fd fragment consisting of the VH and CHl domains; (iv) a Fv fragment consisting of the VL and VH domains of a single arm of an antibody, (v) a dAb fragment (Ward et al. , (1989) Nature 341 :544-546), which consists of a VH domain; and (vi) an isolated complementarity determining region (CDR). Single chain antibodies are also included by reference in the term "antibody."

[0066] The terms "array" or "microarray" or "biochip" or "chip" as used herein is a plurality of target elements, each target element comprising a defined amount of one or more polypeptides (including antibodies) or nucleic acids immobilized onto a defined area of a substrate surface, as discussed in further detail, below.

[0067] The term "expression cassette" as used herein refers to a nucleotide sequence which is capable of affecting expression of a structural gene (i.e., a protein coding sequence, such as an recombinant or isolated protein encoded by a nucleic acid of the invention) in a host compatible with such sequences. Expression cassettes include at least a promoter operably linked with the polypeptide coding sequence; and, optionally, with other sequences, e.g., transcription termination signals. Additional factors necessary or helpful in effecting expression may also be used, e.g., enhancers. Thus, expression cassettes also include plasmids, expression vectors, recombinant viruses, any form of recombinant "naked DNA" vector, and the like.

[0068] "Operably linked" as used herein refers to a functional relationship between two or more nucleic acid (e.g., DNA) segments. Typically, it refers to the functional relationship of transcriptional regulatory sequence to a transcribed sequence. For example, a promoter is operably linked to a coding sequence, such as a nucleic acid of the invention, if it stimulates or modulates the transcription of the coding sequence in an appropriate host cell or other expression system. Generally, promoter transcriptional regulatory sequences that are operably linked to a transcribed sequence are physically contiguous to the transcribed sequence, i.e., they are cis-acύng. However, some transcriptional regulatory sequences, such as enhancers, need not be physically contiguous or located in close proximity to the coding sequences whose transcription they enhance.

[0069] A "vector" comprises a nucleic acid which can infect, transfect, transiently or permanently transduce a cell. It will be recognized that a vector can be a naked nucleic acid, or a nucleic acid complexed with protein or lipid. The vector optionally comprises viral or bacterial nucleic acids and/or proteins, and/or membranes (e.g., a cell membrane, a viral lipid envelope, etc.). Vectors include, but are not limited to replicons (e.g., RNA replicons, bacteriophages) to which fragments of DNA may be attached and become replicated. Vectors thus include, but are not limited to RNA, autonomous self-replicating circular or linear DNA or RNA (e.g., plasmids, viruses, and the like, see, e.g., U.S. Patent No. 5,217,879), and include both the expression and non-expression plasmids. Where a recombinant microorganism or cell culture is described as hosting an "expression vector" this includes both extra-chromosomal circular and linear DNA and DNA that has been incorporated into the host chromosome(s). Where a vector is being maintained by a host cell, the vector may either be stably replicated by the cells during mitosis as an autonomous structure, or is incorporated within the host's genome. '

[0070] As used herein, the term "promoter" includes all sequences capable of driving transcription of a coding sequence in a cell, e.g., a plant cell. Thus, promoters used in the constructs of the invention include cw-acting transcriptional control elements and regulatory sequences that are involved in regulating or modulating the timing and/or rate of transcription of a gene. For example, a promoter can be a cis- acting transcriptional control element, including an enhancer, a promoter, a transcription terminator, an origin of replication, a chromosomal integration sequence, 5' and 3' untranslated regions, or an intronic sequence, which are involved in transcriptional regulation. These cis-acting sequences typically interact with proteins or other biomolecules to carry out (turn on/off, regulate, modulate, etc.) transcription. "Constitutive" promoters are those that drive expression continuously under most environmental conditions and states of development or cell differentiation. "Inducible" or "regulatable" promoters direct expression of the nucleic acid of the invention under the influence of environmental conditions or developmental conditions. Examples of environmental conditions that may affect transcription by inducible promoters include anaerobic conditions, elevated temperature, drought, or the presence of light. [0071] "Plasmids" can be commercially available, publicly available on an unrestricted basis, or can be constructed from available plasmids in accord with published procedures. Equivalent plasmids to those described herein are known in the art and will be apparent to the ordinarily skilled artisan. [0072] The term "gene" includes a nucleic acid sequence comprising a segment of

DNA involved in producing a transcription product (e.g., a message), which in turn is translated to produce a polypeptide chain, or regulates gene transcription, reproduction or stability. Genes can include regions preceding and following the coding region, such as leader and trailer, promoters and enhancers, as well as, where applicable, intervening sequences (introns) between individual coding segments (exons).

[0073] The phrases "nucleic acid" or "nucleic acid sequence" includes oligonucleotide, nucleotide, polynucleotide, or to a fragment of any of these, to DNA or RNA (e.g., mRNA, rRNA. tRNA) of genomic or synthetic origin which may be single- stranded or double-stranded and may represent a sense or antisense strand, to peptide nucleic acid (PNA), or to any DNA-like or RNA-like material, natural or synthetic in origin, including, e.g., iRNA, ribonucleoproteins (e.g., iRNPs). The term encompasses nucleic acids, i.e., oligonucleotides, containing known analogues of natural nucleotides. The term also encompasses nucleic-acid-like structures with synthetic backbones, see e.g., Mata (1997) Toxicol. Appl. Pharmacol. 144:189-197; Strauss-Soukup (1997) Biochemistry 36:8692-8698; Samstag (1996) Antisense Nucleic Acid Drug Dev 6: 153- 156.

[0074] "Amino acid" or "amino acid sequence" include an oligopeptide, peptide, polypeptide, or protein sequence, or to a fragment, portion, or subunit of any of these, and to naturally occurring or synthetic molecules. The terms "polypeptide" and "protein" include amino acids joined to each other by peptide bonds or modified peptide bonds, i.e., peptide isosteres, and may contain modified amino acids other than the 20 gene-encoded amino acids. The term "polypeptide" also includes peptides and polypeptide fragments, motifs and the like. The term also includes glycosylated polypeptides. The peptides and polypeptides of the invention also include all "mimetic" and "peptidomimetic" forms, as described in further detail, below.

[0075] As used herein, the term "biologically active fragment thereof thereof refers to a fragment of the protein or nucleic acid that retains at least one biological activity of the whole sequence. In one embodiment, the fragment retains all of the relevant biological activities of the protein or nucleic acid sequence. For example, the biologically active fragment of a protein is one that protects the organism growing in aquaculture from one or more deleterious effects of a pathogen, preferably a virus.

[0076] The term "isolated" includes a material removed from its original environment, e.g., the natural environment if it is naturally occurring. For example, a naturally occurring polynucleotide or polypeptide present in a living animal is not isolated, but the same polynucleotide or polypeptide, separated from some or all of the coexisting materials in the natural system, is isolated. Such polynucleotides could be part of a vector and/or such polynucleotides or polypeptides could be part of a composition, and still be isolated in that such vector or composition is not part of its natural environment. As used herein, an isolated material or composition can also be a "purified" composition, i.e., it does not require absolute purity; rather, it is intended as a relative definition. Individual nucleic acids obtained from a library can be conventionally purified to electrophoretic homogeneity. In alternative aspects, the invention provides nucleic acids which have been purified from genomic DNA or from other sequences in a library or other environment by at least one, two, three, four, five or more orders of magnitude.

[0077] As used herein, the term "recombinant" can include nucleic acids adjacent to a "backbone" nucleic acid to which it is not adjacent in its natural environment. In one aspect, nucleic acids represent 5% or more of the number of nucleic acid inserts in a population of nucleic acid "backbone molecules." "Backbone molecules" according to the invention include nucleic acids such as expression vectors, self-replicating nucleic acids, viruses, integrating nucleic acids, and other vectors or nucleic acids used to maintain or manipulate a nucleic acid insert of interest. In one aspect, the (isolated, recombinant, enriched) nucleic acids represent 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more of the number of nucleic acid inserts in the population of recombinant backbone molecules. "Recombinant" polypeptides or proteins refer to polypeptides or proteins produced by recombinant DNA techniques; e.g., produced from cells transformed by an exogenous DNA construct encoding the desired polypeptide or protein. "Synthetic" polypeptides or protein are those prepared by chemical synthesis, as described in further detail, below. [0078] A promoter sequence can be "operably linked to" a coding sequence when RNA polymerase which initiates transcription at the promoter will transcribe the coding sequence into mRNA, as discussed further, below.

[0079] "Oligonucleotide" includes either a single stranded polydeoxynucleotide or two complementary polydeoxynucleotide strands which may be chemically synthesized. Such synthetic oligonucleotides have no 5' phosphate and thus will not ligate to another oligonucleotide without adding a phosphate with an ATP in the presence of a kinase. A synthetic oligonucleotide can ligate to a fragment that has not been dephosphorylated. [0080] The phrase "substantially identical" in the context of two nucleic acids or polypeptides, can refer to two or more sequences that have, e.g., at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more nucleotide or amino acid residue (sequence) identity, when compared and aligned for maximum correspondence, as measured using one any known sequence comparison algorithm, as discussed in detail below, or by visual inspection. In alternative aspects, the invention provides nucleic acid and polypeptide sequences having substantial identity to an exemplary sequence of the invention over a region of at least about 10, 20, 30, 40, 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000 or more residues, or a region ranging from between about 50 residues to the full length of the nucleic acid or polypeptide. Nucleic acid sequences of the invention can be substantially identical over the entire length of a polypeptide coding region. [0081] A "substantially identical" amino acid sequence also can include a sequence that differs from a reference sequence by one or more conservative or non-conservative amino acid substitutions, deletions, or insertions, particularly when such a substitution occurs at a site that is not the active site of the molecule, and provided that the polypeptide essentially retains its functional properties. A conservative amino acid substitution, for example, substitutes one amino acid for another of the same class (e.g., substitution of one hydrophobic amino acid, such as isoleucine, valine, leucine, or methionine, for another, or substitution of one polar amino acid for another, such as substitution of arginine for lysine, glutamic acid for aspartic acid or glutamine for asparagine). One or more amino acids can be deleted, for example, from a recombinant or isolated protein encoded by a nucleic acid of the invention, resulting in modification of the structure of the polypeptide, without significantly altering its biological activity. For example, amino- or carboxyl-terminal amino acids that are not required for the protein's activity can be removed. [0082] "Hybridization" includes the process by which a nucleic acid strand joins with a complementary strand through base pairing- Hybridization reactions can be sensitive and selective so that a particular sequence of interest can be identified even in samples in which it is present at low concentrations. Stringent conditions can be defined by, for example, the concentrations of salt or formamide in the prehybridization and hybridization solutions, or by the hybridization temperature, and are well known in the art. For example, stringency can be increased by reducing the concentration of salt, increasing the concentration of formamide, or raising the hybridization temperature, altering the time of hybridization, as described in detail, below. In alternative aspects, nucleic acids of the invention are defined by their ability to hybridize under various stringency conditions (e.g., high, medium, and low), as set forth herein.

[0083] "Variant" includes polynucleotides or polypeptides of the invention modified at one or more base pairs, codons, introns, exons, or amino acid residues (respectively) yet still retain the biological activity of the recombinant polypeptide of the invention. [0084] As used herein, the term "therapeutic" refers to a protein or biologically active fragment thereof, or a nucleic acid sequence encoding the protein or fragment thereof that can heal, or provide a remedial, palliative, or preventive effect on a pathologic process. Therapeutic substances and compounds can be used to treat medical diseases, disorders, conditions, or syndromes. [0085] As used herein, the term "macroalgae" refers to algae that form structures easily discernable with the naked eye in at least one life stage. Usually these organisms have secondary vascularization and organs. Examples of different groups containing macroalgae include, but are not limited to, the chlorophyta, rhodophyta, and phaeophyta. [0086] As used herein, the term "microalgae" include both prokaryotic and eukaryotic algae that are classed in many different genera. Prokaryotic algae are typically referred to as cyanobacteria or bluegreen algae. Eukaryotic microalgae come from many different genera, some of which overlap with the macroalgae, but can be generally differentiated by their size and lack of defined organs. Microalgae can have specialized cell types. Examples of different groups containing microalgae include, but are not limited to the chlorophyta, rhodophyta, phaeophyta, dinophyta, euglenophyta, cyanophyta, prochlorophyta, and cryptophyta.

[0087] As used herein, the term "up-regulated" refers to an increase of mRNA or protein expression in a crustacean infected with a pathogen, preferably a virus, when compared to an uninfected crustacean.

[0088] As used herein, the term "down-regulated" refers to a decrease of mRNA or protein expression in a crustacean infected with a pathogen, preferably a virus, when compared to an uninfected crustacean.

ESTs, nucleic acids, polypeptides, and fragments thereof

[0089] The invention provides compositions consisting of, or comprising, a set of differentially expressed gene selected by their differential expression in viral-infected shrimp relative to non-virally-infected, or healthy shrimp, e.g., a set of genes comprising sequences of the invention. Summary of EST clones isolated from subtractive libraries of healthy and white spot syndrome virus fWSSWinfected shrimp.

Healthy Infected

Number of cDNA clones sequenced 478 478 Number of contigs 43 57 Number of unassembled clones/ singletons 42 64 Number of unigenes 85 121

[0090] Expressed sequence tag (EST) analysis is an effective, comprehensive, and relatively straightforward method of examining gene expression. This method is particularly useful for species where no or limited information is available regarding the genome of the species. An EST approach was used to examine gene expression in different tissues (cephalothorax, eyestalks, and pleopod) in black tiger shrimp, P. monodon (Lehnert et ah, 1999). The putative identities of many of these ESTs revealed the occurrence of tissue-specific expression that includes novel genes. A number of immune genes have also been isolated from the hemocyte and hepatopancreas cDNA libraries from cultured specific pathogen free (SPF) Penaeus (Litopenaeus) vannamei shrimp and wild P. setiferus (Gross et at , 2001). Among these shrimp, anti-microbial peptides and lectins were most prevalent in the hemocyte and hepatopancreas cDNA libraries, respectively (Gross et al., 2001). Immune genes isolated by EST analysis from a hemocyte cDNA library of P. monodon include genes that are involved in the clotting system and the prophenoloxidase-activating system, as well as antioxidative enzymes, antimicrobial peptides, and serine protease inhibitors (Supungul et al. , 2002). Recently, Rojtinnakorn et al. (2002) compared the mRNA expression profiles of healthy and WSSV-infected kuruma prawns (Penaeus japonicus) by EST analysis of hemocytes. Hemocytes and hepatopancreas tissue represent the core of the primitive immune system found in shrimp and most other invertebrates. Humoral immune responses are initiated in the shrimp hepatopancreas while both humoral and cellular components of the immune system are found in the hemocyte population. Applicants are unaware of any prior analysis of hematopancreatic gene expression in the presence or absence of viral infection in shrimp.

[0091] Thus, one aspect of the present invention is a composition of ESTs representing differentially expressed genes and substantially identical nucleic acids in the presence and absence of infection in crustaceans. Any suitable crustacean can be analyzed. In one embodiment, the crustacean is a shrimp. In a preferred embodiment, the shrimp is a P. vannamei shrimp. Any suitable type of infection can be analyzed. In one embodiment, the infection is a viral infection. The virus can be white spot syndrome virus, Taura syndrome virus, infectious hypodermal and hematopoietic virus, yellowhead virus or baculovirus penae. In a preferred embodiment, the virus is a member of the genus Whispovirus. Most preferably, the virus is white spot syndrome virus (WSSV). Differential expression of genes include, but are not limited to genes that are up or down regulated relative to the genes expressed in a non-infected crustacean. [0092] In one embodiment, differential gene expression is determined using ESTs isolated from cDNA libraries of virally-infected shrimp and non-virally infected shrimp. The gene expression can be determined using the hepatopancreas tissue or the hemocyte population. Preferrably, the hepatopancreas tissue is the source of the ESTs. Any suitable numbers of ESTs may be considered. [0093] Gene expression can be analyzed at any time during infection, including but not limited to time periods representing the onset of infection or the resolving of infection as well as during acute and chronic infections. In one embodiment, the EST isolated specifically correspond to the onset of infection, particularly that of viral disease. It is contemplated that gene expression analysis may also be performed if the crustacean has more than one infective entity.

[0094] In a specific embodiment, the ESTs are isolated from shrimp that non- infected or infected with WSSV at the onset of viral infection. [0095] Therefore, the invention provides isolated and recombinant nucleic acids, including ESTs. The invention further provides probes comprising or consisting of nucleic acids, e.g., ESTs₃ of the invention.

[0096] The invention provides isolated or recombinant nucleic acids comprising a nucleic acid sequence having at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more, or complete (100%) sequence identity to an exemplary nucleic acid of the invention over a region of at least about 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000, 1050, 1100, 1150, 1200, 1250, 1300, 1350, 1400, 1450, 1500, 1550 or more, residues. ^• In one aspect, the nucleic acid encodes at least one polypeptide having a biologic activity, and the sequence identities are determined by analysis with a sequence comparison algorithm or by a visual inspection. In another aspect, the invention provides nucleic acids for use as probes, inhibitory molecules {e.g., antisense, iRNAs), transcriptional or translational regulation, and the like. Exemplary nucleic acids of the invention include isolated or recombinant nucleic acids comprising a nucleic acid sequence as set forth in Appendix A, and subsequences thereof, e.g., at least about 10, 15, 20, 25, 30, 35, 40, 45, 50, 75, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000, 1050, 1100, 1150, 1200, 1250, 1300,

1350, 1400, 1450, 1500 or more residues in length, or over the full length of a gene or transcript.

[0097] Another aspect of the invention is an isolated or recombinant nucleic acid including at least 10 consecutive bases of a nucleic acid sequence of the invention, sequences substantially identical thereto, and the sequences complementary thereto.

[0098] The invention provides isolated or recombinant nucleic acids comprising a sequence that hybridizes under stringent conditions to a nucleic acid of the invention, e.g., any one of the sequences in Appendix A. The nucleic acid can be at least about 50, 75, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000, 1050,- 1100, 1150, 1200, 1250, 1300, 1350, 1400, 1450, 1500 or more residues in length or the full length of the gene or transcript. In one aspect, the stringent conditions include a wash step comprising a wash in 0.1X SSC at a temperature of about 65°C for about 15 minutes. [0099] In a specific embodiment, the gene of interest is a lectin gene. Lectin are known to plays a critical role in the innate immunity in vertebrates by activating complement factor after binding to the carbohydrate moieties on the surfaces of viral capsid proteins (Vorup- Jensen et al, 2000). In humans, the C-type lectin exhibits CD4-independent binding of the envelope glycoprotein, gpl20, of human immunodefficiency virus (HIV) (Curtis et al, 1992). Human MBL was also shown to bind to the envelope proteins of the influenza A virus, neutralizing the virus by inhibiting the spread of the virus and simultaneously activating the complement cascade (Kase et al, 1999; Malhotra et al, 1994). Replacement of MBL to MBL- deficient human has shown encouraging results in enhancing complement activation ability and opsonic activity towards Saccharomyces cerevisae in the treated individuals (reviewed in Kilpatric, 2002). In invertebrates (tunicate Clavelina picta), the binding of MBL to microbial ligands activates the complement component C3, through an MBL- associated serine proteinase. This leads to phagocytosis of the opsonized target and/ or humoral cell killing via the assembly of a membrane attack complex (Vasta et al , 1999). Therefore, lectins with such activity are suitable for use in the prevention and treatment of infection in invertebrates.

[0100] The nucleic acids of the invention can be made, isolated and/or manipulated by, e.g., cloning and expression of cDNA libraries, amplification of message or genomic DNA by PCR, and the like. Techniques for the manipulation of nucleic acids, such as, e.g., subcloning, labeling probes (e.g., random-primer labeling using Klenow polymerase, nick translation, amplification), sequencing, hybridization and the like are well described in the scientific and patent literature, see, e.g., Sambrook, ed., MOLECULAR CLONING: A LABORATORY MANUAL (2ND ED.), VoIs. 1-3, Cold Spring Harbor Laboratory, (1989); CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, Ausubel, ed. John Wiley & Sons, Inc., New York (1997); LABORATORY TECHNIQUES IN BIOCHEMISTRY AND MOLECULAR BIOLOGY: HYBRIDIZATION WITH NUCLEIC ACID PROBES, Part I. Theory and Nucleic Acid Preparation, Tijssen, ed. Elsevier, N. Y. (1993). [0101] The invention provides isolated or recombinant nucleic acids that hybridize under stringent conditions to an exemplary sequence of the invention, or a nucleic acid that encodes a polypeptide of the invention. The stringent conditions can be highly stringent conditions, medium stringent conditions, low stringent conditions, including the high and reduced stringency conditions described herein. In one aspect, it is the stringency of the wash conditions that set forth the conditions which determine whether a nucleic acid is within the scope of the invention, as discussed below.

[0102] In alternative embodiments, nucleic acids of the invention as defined by their ability to hybridize under stringent conditions can be between about five residues and the full length of nucleic acid of the invention; e.g., they can be at least 5, 10, 15, 20, 25, 30, 35, 40, 50, 55, 60, 65, 70, 75, 80, 90, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000, or more, residues in length. Nucleic acids shorter than full length are also included. These nucleic acids can be useful as, e.g., hybridization probes, labeling probes, PCR oligonucleotide probes, iRNA, antisense or sequences encoding antibody binding peptides (epitopes), motifs, active sites and the like.

[0103] In one aspect, nucleic acids of the invention are defined by their ability to hybridize under high stringency comprises conditions of about 50% formamide at about 37°C to 42°C. In one aspect, nucleic acids of the invention are defined by their ability to hybridize under reduced stringency comprising conditions in about 35% to 25% formamide at about 30°C to 35°C.

[0104] Alternatively, nucleic acids of the invention are defined by their ability to hybridize under high stringency comprising conditions at 42°C in 50% formamide, 5X SSPE, 0.3% SDS, and a repetitive sequence blocking nucleic acid, such as cot-1 or salmon sperm DNA (e.g., 200 n/ml sheared and denatured salmon sperm DNA). In one aspect, nucleic acids of the invention are defined by their ability to hybridize under reduced stringency conditions comprising 35% formamide at a reduced temperature of 35°C.

[0105] Following hybridization, the filter may be washed with 6X SSC, 0.5% SDS at 5O⁰C. These conditions are considered to be "moderate" conditions above 25% formamide and "low" conditions below 25% formamide. A specific example of "moderate" hybridization conditions is when the above hybridization is conducted at 30% formamide. A specific example of "low stringency" hybridization conditions is when the above hybridization is conducted at 10% formamide. [0106] The temperature range corresponding to a particular level of stringency can be further narrowed by calculating the purine to pyrimidine ratio of the nucleic acid of interest and adjusting the temperature accordingly. Nucleic acids of the invention are also defined by their ability to hybridize under high, medium, and low stringency conditions as set forth in Ausubel and Sambrook. Variations on the above ranges and conditions are well known in the art. Hybridization conditions are discussed further, below.

[0107] The above procedure may be modified to identify nucleic acids having decreasing levels of homology to the probe sequence. For example, to obtain nucleic acids of decreasing homology to the detectable probe, less stringent conditions may be used. For example, the hybridization temperature may be decreased in increments of 5°C from 68°C to 42°C in a hybridization buffer having a Na⁺ concentration of approximately IM. Following hybridization, the filter may be washed with 2X SSC, 0.5% SDS at the temperature of hybridization. These conditions are considered to be "moderate" conditions above 50⁰C and "low" conditions below 50⁰C. A specific example of "moderate" hybridization conditions is when the above hybridization is conducted at 55°C. A specific example of "low stringency" hybridization conditions is when the above hybridization is conducted at 45°C.

[0108] The invention also provides nucleic acid probes that can be used, e.g., for identifying nucleic acids encoding a polypeptide with a biologic activity or fragments thereof or for identifying genes. In one aspect, the probe comprises at least 10 consecutive bases of a nucleic acid of the invention. Alternatively, a probe of the invention can be at least about 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 110, 120, 130, 150 or about 10 to 50, about 20 to 60 about 30 to 70, consecutive bases of a sequence as set forth in a nucleic acid of the invention. The probes identify a nucleic acid by binding and/or hybridization. The probes can be used in arrays of the invention, see discussion below, including, e.g., capillary arrays. The probes of the invention can also be used to isolate other nucleic acids or polypeptides. [0109] The invention provides expression vectors and cloning vehicles comprising nucleic acids of the invention, e.g., sequences encoding the recombinant or isolated proteins encoded by the nucleic acids of the invention. Expression vectors and cloning vehicles of the invention can comprise viral particles, baculo virus, phage, plasmids, phagemids, cosmids, fosmids, bacterial artificial chromosomes, viral DNA (e.g., vaccinia, adenovirus, foul pox virus, pseudorabies and derivatives of SV40), Pl -based artificial chromosomes, yeast plasmids, yeast artificial chromosomes, and any other vectors specific for specific hosts of interest (such as bacillus, Aspergillus and yeast). In one embodiment, the expression vectors and cloning vehicle comprise yeast plasmids. Vectors of the invention can include chromosomal, non-chromosomal and synthetic DNA sequences. Large numbers of suitable vectors are known to those of ^■ skill in the art, and are commercially available.

[0110] In one embodiment, the vector is a baculovirus. In a specific embodimennt the baculovirus is Autographica californica nuclear polyhedrosis virus (AcNPV). Vectors and molecular biology supplies, as well as methods for baculovirus expression vector systems, including AcNPV, are readily available from commercial suppliers

[0111] The expression vector can comprise a promoter, a ribosome binding site for translation initiation and a transcription terminator. The vector may also include appropriate sequences for amplifying expression. Mammalian expression vectors can comprise an origin of replication, any necessary ribosome binding sites, a polyadenylation site, splice donor and acceptor sites, transcriptional termination sequences, and 5' flanking non-transcribed sequences. In some aspects, DNA sequences derived from the SV40 splice and polyadenylation sites may be used to provide the required non-transcribed genetic elements.

[0112] In one aspect, the expression vectors contain one or more selectable marker genes to permit selection of host cells containing the vector.

[0113] Vectors for expressing the polypeptide or fragment thereof in eukaryotic cells can also contain enhancers to increase expression levels. Enhancers are cis-acting elements of DNA, usually from about 10 to about 300 bp in length that act on a promoter to increase its transcription. [0114] The invention provides cloning vehicles comprising an expression cassette

(e.g., a vector) of the invention or a nucleic acid of the invention. The cloning vehicle can be a viral vector, a baculovirus, a plasmid, a phage, a phagemid, a cosmid, a fosmid, a bacteriophage or an artificial chromosome. The viral vector can comprise an adenovirus vector, a retroviral vector or an adeno-associated viral vector. The cloning vehicle can comprise a bacterial artificial chromosome (BAC), a plasmid, a bacteriophage Pl -derived vector (PAC), a yeast artificial chromosome (YAC), or a mammalian artificial chromosome (MAC).

[0115] The invention provides transformed cell comprising a nucleic acid of the invention or an expression cassette (e.g., a vector) of the invention, or a cloning vehicle of the invention. In one aspect, the transformed cell can be a bacterial cell, a mammalian cell, a fungal cell, a yeast cell, an insect cell or a plant cell.

[0116] In another aspect, provided herein are recombinant or isolated proteins derived from full-length gene or recombinant truncated amino acids derived from the functional domain of the gene identified using the EST differential libraries provided herein. The identified ESTs of interest can be used to clone full length cDNAs using methods known in the art. See e.g., U.S. Patent No. 6,265,165. Recombinant polypeptides generated from these nucleic acids can be individually isolated or cloned and tested for a desired activity. Any recombinant expression system can be used, including bacterial, mammalian, yeast, insect or plant cell expression systems.

Recombinant truncated proteins of interest, are those that retain at least a detectable amount of the desired biologic activity.

[0117] The invention provides fusion proteins and nucleic acids encoding them. A polypeptide of the invention can be fused to a heterologous peptide or polypeptide, such as N-terminal identification peptides which impart desired characteristics, such as increased stability or simplified purification. Peptides and polypeptides of the invention can also be synthesized and expressed as fusion proteins with one or more additional domains linked thereto for, e.g., producing a more protective peptide, to more readily isolate a recombinantly synthesized peptide, to identify and isolate antibodies and antibody-expressing B cells, and the like.

[0118] In practicing the invention, nucleic acids of the invention and nucleic acids encoding the polypeptides of the invention, or modified nucleic acids of the invention, can be reproduced by amplification. Amplification can also be used to clone or modify the nucleic acids of the invention. Thus, the invention provides amplification primer sequence pairs for amplifying nucleic acids of the invention. One of skill in the art can design amplification primer sequence pairs for any part of or the full length of these sequences.

[0119] The invention provides methods of producing a recombinant polypeptide comprising the steps of: (a) providing a nucleic acid of the invention operably linked to a promoter; and (b) expressing the nucleic acid of step (a) under conditions that allow expression of the polypeptide, thereby producing a recombinant polypeptide. In one aspect, the method can further comprise transforming a host cell with the nucleic acid of step (a) followed by expressing the nucleic acid of step (a), thereby producing a recombinant polypeptide in a transformed cell. [0120] Any suitable host cell can be used for the production of the recombinant or isolated protein or biologically active fragment thereof.

[0121] The invention provides isolated or recombinant antibodies that specifically bind to the recombinant polypeptides of the invention. These antibodies can be used to isolate, identify or quantify the recombinant polypeptides of the invention or related polypeptides. These antibodies can be used to isolate other polypeptides within the scope the invention. The antibodies can be designed to bind to an active site of a recombinant polypeptide. Thus, the invention provides methods of inhibiting polypeptides using the antibodies of the invention. [0122] The antibodies can be used in immunoprecipitation, staining, immunoaffinity columns, and the like. If desired, nucleic acid sequences encoding for specific antigens can be generated by immunization followed by isolation of polypeptide or nucleic acid, amplification or cloning and immobilization of polypeptide onto an array of the invention. Alternatively, the methods of the invention can be used to modify the structure of an antibody produced by a cell to be modified, e.g., an antibody's affinity can be increased or decreased. Furthermore, the ability to make or modify antibodies can be a phenotype engineered into a cell by the methods of the invention.

[0123] Methods of immunization, producing and isolating antibodies (polyclonal and monoclonal) are known to those of skill in the art and described in the scientific and patent literature, see, e.g., Coligan, CURRENT PROTOCOLS IN

IMMUNOLOGY, Wiley/Greene, NY (1991); Stites (eds.) BASIC AND CLINICAL IMMUNOLOGY (7th ed.) Lange Medical Publications, Los Altos, CA ("Stites"); Goding, MONOCLONAL ANTIBODIES: PRINCIPLES AND PRACTICE (2d ed.) Academic Press, New York, NY (1986); Kohler (1975) Nature 256:495; Harlow (1988) ANTIBODIES, A LABORATORY MANUAL, Cold Spring Harbor Publications, New York. Antibodies also can be generated in vitro, e.g., using recombinant antibody binding site expressing phage display libraries, in addition to the traditional in vivo methods using animals. See, e.g., Hoogenboom (1997) Trends Biotechnol. 15:62-70; Katz (1997) Annu. Rev. Biophys. Biomol. Struct. 26:27-45. [0124] Polyclonal antibodies generated against the polypeptides of the invention can be obtained by direct injection of the polypeptides into an animal or by administering the polypeptides to a non-human animal. The antibody so obtained will then bind the polypeptide itself. In this manner, even a sequence encoding only a fragment of the polypeptide can be used to generate antibodies which may bind to the whole native polypeptide. Such antibodies can then be used to isolate the polypeptide from cells expressing that polypeptide.

[0125] For preparation of monoclonal antibodies, any technique which provides antibodies produced by continuous cell line cultures can be used. Examples include the hybridoma technique, the trioma technique, the human B-cell hybridoma technique, and the EBV-hybridoma technique (see, e.g., Cole (1985) in Monoclonal Antibodies and Cancer Therapy, Alan R. Liss, Inc., pp. 77-96).

Uses of Recombinant Nucleic Acids and Polypeptides

Methods of treating infection [0126] Another aspect of the invention is a method of protecting crustacean from infection using sequence of the invention, e.g., recombinant or isolated protein or nucleic acids derived from the full-length gene or recombinant truncated protein or nucleic acids derived from the functional domain of the gene identified using the EST differential libraries of the instant invention. In one embodiment, the infection is a viral infection. In a specific embodiment, the viral infection is WSSV. The infection can also be one caused by bacteria, fungi, and the like. In one embodiment, the crustacean is a shrimp. The recombinant or isolated protein or nucleic acid, or biologically active fragment thereof, can be administered in any suitable manner in a single dose or repeatedly. The recombinant or isolated protein or nucleic acid can be administered alone or in combination with other treatment modalities for the infection. A therapeutically effective amount of recombinant polypeptide or biolgically active fragment thereof is an amount that reduces or eliminates at least one symptom of the infection. In one embodiment, a therapeutically effective amount prevents infection or cures established infection. [0127] The invention provides nucleic acids complementary to (e.g., antisense sequences to, including ribozymes and siRNA, in addition to "traditional" antisense sequences) the nucleic acids of the invention, e.g., as in Appendix A, for therapeutic (antiviral) and diagnostic purposes. In one aspect, the sequences of the invention are used as double-stranded inhibitory RNA (RNAi, or RNA interference) molecules (including small interfering RNA, or siRNAs, for inhibiting transcription, and microRNAs, or miRNAs, for inhibiting translation) comprising a sequence of the invention (e.g., as set forth in Appendix A, below) or a subsequence of a sequence of the invention. In one aspect, delivery of RNA (RNAi, or RNA interference) molecules is by plasmids expressing inhibitory sequences; and in one aspect follows published methods for DNA plasmid vaccine oral delivery (for example see Ramos et al 2005 Marine Biotechnology 7:89-94 for oral delivery of plasmid DNA to express proteins in tilapia). Another exemplary method uses DNA vaccines for oral delivery based on dendrimer complexed into nanoparticles, e.g., as described in Bodnar et al 2005 Biomacromol 6:2521-27.

[0128] The protein or biologically active fragment thereof can be one that is upregulated or downregulated following infection with a pathogen. The up-regulation can be mediated by any mechanism including, but not limited to increased transcription, increased mRNA stability, decreased mRNA degradation, or increased translation. Similarly, the down-regulation can be mediated by any mechanism known in the art. Expression can be determined using routine methods. Exemplary methods include those disclosed in CURRENT PROTOCOLS IN MOLECULAR BIOLOGY (Ausubel et al., John Wiley & Sons, most recent edition). Infection with pathogen is readily determined using routine methods that include but are not limited to bioassays using indicator hosts, monitoring clinical signs, histopathology, dot blot and in situ hybridization using a virus specific probe, and PCR. See, e.g., Dhar et al., J. Clin. Microbiol. 39:2835-45 (2001).

[0129] In one embodiment, the protein or biologically active fragment is one that enhances the innate, non-adaptive immunity of the organism. For example, in shrimp, immunity consists of a humoral and a cellular response. Cellular responses involve phagocytosis, nodule formation and encapsulation. Humoral responses involve the prophenoloxidase-activating cascade and immune-related proteins such as lysozymes, lectins, and anti-microbial peptides. See, e.g., Soderhall, Dev. Comp. Immunol. 23:263- 6 (1999).

[0130] It is the object of the invention to provides a method of transfecting or infecting crustaceans with non-native therapeutic proteins using baculovirus. This method is particularly suitable for crustaceans in aquaculture. Preferably, the crustaceans are Pacific white shrimp (Penaeus vannamei) and the baculovirus is Autographa californica nuclear polyhedrosis virus (AcNPV). The crustacean can be infected either by injection or orally by incorporating the virus into the crustacean's food. The baculovirus can be engineered to express green fluorescent protein (GFP) for monitoring infection. For example, the therapeutic proteins can inhibit the growth or replication of bacteria (e.g., Vibrio) or viruses (e.g., Taura or White Spot virus). [0131] In one aspect of the present invention, the crustacean can be provided a feed or feed additive which incorporates recombinant or isolated protein or nucleic acids identified using the EST differential libraries of the instant invention as a prophylactic or therapeutic treatment for the deleterious effects of the virus on the host. Thus, the present invention provides for a composition comprising at least one recombinant or isolated protein or nucleic acid, or biologically active fragment thereof, in a food source for the crustacean. The invention provides edible enzyme delivery matrices comprising a polypeptide of the invention, e.g., a polypeptide encoded by the nucleic acid of the invention. The protein or biologically active fragment thereof can be provided in a cell or in a disrupted cell. Any suitable cell can be employed including, but not limited to microalgae and macroalgae cells. The cells can be disrupted using any suitable methods.

[0132] The bioactive food complex can be preserved without drying as a semi-solid, moist paste, not a liquid, composed of microcapsules or beads, or as moist noodles, pellets, sheets or other forms, or can be stored frozen by employing cry ©preservatives. See, e.g., U.S. Patent No. 5,698,246; 4,741,904. The bioactive food complex can be added directly to aquaculture animal containment systems to be eaten by aquatic animals. In one embodiment of the invention, the bioactive food complex or the primary emulsion of the bioactive food complex can be added to pelleted or extruded aquatic feeds as a top-dress coating or enrobing of the pelleted or extruded aquatic feed.

[0133] The foodstuffs incorporated into the particulate fish foods of this invention may be those which are normally used for other particulate fish foods. Frequently fish meal will provide at least 30% of the formulation and often it will provide at least 50% of the formulation. [0134] Particulate fish foods are customarily manufactured by three routes, all of which involve some form of extrusion through a die. The methods are normally classified as compressed pelleting (also known as steam pelleting),/extruded pelleting and moist pelleting.

[0135] The feed of the invention may be adjusted for the requirements of the marine animal being fed as is known. For shrimp, the feed comprises animal protein, brine shrimp, egg product, betaine, alanine, isoleucine, leucine, serine, valine, glycine, astaxanthin, vitamin A supplement, vitamin B₁₂ supplement, riboflavin supplement, calcium pantothenate, niacin supplement, vitamin D3 supplement, vitamin E supplement, menadione sodium bisulfite complex, folic acid, biotin, thiamine, pyridoxine hydrochloride, inositol and choline chloride. The particulate feed may also include medicaments. In one aspect, an edible oil is employed. Typical oils include fish oil, peanut oil, olive oil, corn oil, sunflowerseed oil, cotton seed oil, soybean oil, rapeseed oil, coconut oil, and palm oil. The oil may provide the omega-3 HUFA (highly unsaturated fatty acid) dietary requirements of marine shrimp and fish by providing EPA (eicosapentaenoic acid) and DHA (docosahexenoic acid).

[0136] In one embodiment, amplified cDNA can be cloned into bacterial expression vector (such as pETlOO Directional TOPO Expression vector, Invitrogen Inc.) and used to transform BL 21 Star (DE3) One Shot Chemically Competent Escherichia coli (Invitrogen, Inc.) following the manufacturer's protocol. Escherichia coli strain BL21 cells carrying IPTG inducible gene can be grown in LB medium containing ampicillin, and subsequently induced with IPTG to elicit expression of recombinant or isolated protein. The expression of the recombinant or isolated protein will be empirically optimized to obtain maximum induction using routine methods. Bacterial biomass containing cells, which express the shrimp recombinant or isolated protein, are then added to shrimp feed in a free or microbound format (in beads composed of alginate and starch in a polymeric form). Attractants can optionally be added to make the feed or beads more palatable to the target species. In the case of shrimp, krill meal is employed as the attractant. RNAi reagents

[0137] In one aspect, the invention provides an RNA inhibitory molecule, a so- called "RNAi" molecule, comprising a recombinant or isolated protein or nucleic acid sequence of the invention to be administered as a prophylactic or therapeutic regimen for infection. The RNAi molecule comprises a double-stranded RNA (dsRNA) molecule. The RNAi can inhibit expression of a gene encoding a nucleic acid of the invention. In one aspect, the RNAi is about 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or more duplex nucleotides in length. While the invention is not limited by any particular mechanism of action, the RNAi can enter a cell and cause the degradation of a single- stranded RNA (ssRNA) of similar or identical sequences, including endogenous mRNAs. When a cell is exposed to double-stranded RNA (dsRNA), mRNA from the homologous gene is selectively degraded by a process called RNA interference (RNAi). A possible basic mechanism behind RNAi is the breaking of a double-stranded RNA (dsRNA) matching a specific gene sequence into short pieces called short interfering RNA, which trigger the degradation of mRNA that matches its sequence. In one aspect, the RNAi's of the invention are used in gene-silencing therapeutics, see, e.g., Shuey (2002) Drug Discov. Today 7:1040-1046. In one aspect, the invention provides methods to selectively degrade RNA using the RNAi's of the invention. The process may be practiced in vitro, ex vivo or in vivo. In one aspect, the RNAi molecules of the invention can be used to generate a loss-of-function mutation in a cell, an organ or an animal. Methods for making and using RNAi molecules for selectively degrade RNA are well known ϊn the art, see, e.g., U.S. Patent No. 6,506,559; 6,511,824; 6,515,109; 6,489,127.

Functional genomics studies [0138] In yet another aspect of the present invention, a library of ESTs as well as cDNAs are provided for use in functional genomic analysis. In practicing the methods of the invention, a variety of apparatus and methodologies can be used to in conjunction with the polypeptides and nucleic acids of the invention, e.g., to screen polypeptides for biologic activity, to screen compounds as potential modulators, e.g., activators or inhibitors, of an activity of a recombinant polypeptide encoded by a nucleic acid of the invention, for antibodies that bind to a polypeptide of the invention, for nucleic acids that hybridize to a nucleic acid of the invention, to screen for cells expressing a polypeptide of the invention and the like.

[0139] Nucleic acids or polypeptides of the invention can be immobilized to or applied to an array. Arrays can be used to screen for or monitor libraries of compositions (e.g., small molecules, antibodies, nucleic acids, etc.) for their ability to bind to or modulate the activity of a nucleic acid or a polypeptide of the invention. For example, in one aspect of the invention, a monitored parameter is transcript expression of a gene. One or more, or, all the transcripts of a cell can be measured by hybridization of a sample comprising transcripts of the cell, or, nucleic acids representative of or complementary to transcripts of a cell, by hybridization to immobilized nucleic acids on an array, or "biochip." By using an "array" of nucleic acids on a microchip, some or all of the transcripts of a cell can be simultaneously quantified. Alternatively, arrays comprising genomic nucleic acid can also be used to determine the genotype of a newly engineered strain made by the methods of the invention. "Polypeptide arrays" can also be used to simultaneously quantify a plurality of proteins. The present invention can be practiced with any known "array," also referred to as a "microarray" or "nucleic acid array" or "polypeptide array" or "antibody array" or "biochip," or variation thereof. Arrays are genetically a plurality of "spots" or "target elements," each target element comprising a defined amount of one or more biological molecules, e.g., oligonucleotides, immobilized onto a defined area of a substrate surface for specific binding to a sample molecule, e.g., mRNA transcripts. [0140] In practicing the methods of the invention, any known array and/or method of making and using arrays can be incorporated in whole or in part, or variations thereof, as described, for example, in U.S. Patent Nos. 6,277,628; 6,277,489; 6,261,776; 6,258,606; 6,054,270; 6,048,695; 6,045,996; 6,022,963; 6,013,440; 5,965,452; 5,959,098; 5,856,174; 5,830,645; 5,770,456; 5,632,957; 5,556,752; 5,143,854; 5,807,522; 5,800,992; 5,744,305; 5,700,637; 5,556,752; 5,434,049; see also, e.g., WO 99/51773; WO 99/09217; WO 97/46313; WO 96/17958; see also, e.g., Johnston (1998) Curr. Biol. 8:R171-R174; Schummer (1997) Biotechniques 23:1087- 1092; Kern (1997) Biotechniques 23:120-124; Solinas-Toldo (1997) Genes, Chromosomes & Cancer 20:399-407; Bowtell (1999) Nature Genetics Supp. 21:25-32. See also published U.S. patent applications Nos.20010018642; 20010019827; 20010016322; 20010014449; 20010014448; 20010012537; 20010008765.

Biopesticides

[0141] In another aspect of the invention the differentially expressed ESTs differentially with viral infection are useful as a biopesticide. For example, baculovirus expressing shrimp chitinase gene can be used as a biological agent to infect and kill lepidopteran insects that infect agricultural crops. Baculoviruses have been widely used as a biological control agent in agricultural crop pest management Further effectiveness can be achieved by co-expressing shrimp chitinase gene with the recombinant polypeptide or biologically active fragment thereof, and then spraying this recombinant baculovirus on crop fields infected by an insect pest.

Screening assays

[0142] The invention provides methods for identifying a substrate of a recombinant polypeptide of interest, comprising the following steps: (a) providing a polypeptide of the invention; or a polypeptide encoded by a nucleic acid of the invention; (b) providing a test substrate; and (c) contacting the polypeptide of step (a) with the test substrate of step (b) and detecting a decrease in the amount of substrate or an increase in the amount of reaction product, wherein a decrease in the amount of the substrate or an increase in the amount of a reaction product identifies the test substrate as the polypeptide substrate. [0143] The invention also provides methods of determining whether a test compound specifically hinds to a polypeptide comprising the following steps: (a) expressing a nucleic acid or a vector comprising the nucleic acid under conditions permissive for translation of the nucleic acid to a polypeptide, wherein the nucleic acid comprises a nucleic acid of the invention, or, providing a polypeptide of the invention; (b) providing a test compound; (c) contacting the polypeptide with the test compound; and (d) determining whether the test compound of step (b) specifically binds to the polypeptide.

[0144] The invention provides methods for identifying a modulator of a recombinant polypeptide's activity comprising the following steps: (a) providing a polypeptide of the invention or a polypeptide encoded by a nucleic acid of the invention; (b) providing a test compound; (c) contacting the polypeptide of step (a) with the test compound of step (b) and measuring an activity of the recombinant polypeptide, wherein a change in the recombinant polypeptide activity measured in the presence of the test compound compared to the activity in the absence of the test compound provides a determination that the test compound modulates the recombinant polypeptide activity. In one aspect, the recombinant polypeptide activity can be measured by providing an recombinant polypeptide substrate and detecting a decrease in the amount of the substrate or an increase in the amount of a reaction product, or, an increase in the amount of the substrate or a decrease in the amount of a reaction product. A decrease in the amount of the substrate or an increase in the amount of the reaction product with the test compound as compared to the amount of substrate or reaction product without the test compound identifies the test compound as an activator of recombinant polypeptide activity. An increase in the amount of the substrate or a decrease in the amount of the reaction product with the test compound as compared to the amount of substrate or reaction product without the test compound identifies the test compound as an inhibitor of recombinant polypeptide activity.

Diagnostic Kits [0145] In another aspect of the present invention, methods are provided that use the differentially expressed genes identified in the EST library as a diagnostic tool to evaluate crustaceans in aquaculture and in food processing. Also provided herein is a kit comprising at least one differentially expressed- gene as a nucleic acid or recombinant polypeptide, wherein the kit can be used to positively identify a crustacean as being infected or non-infected with the pathogen of interest. Such kits are also useful for monitoring the course of infection. In one embodiment, the kit comprises at least one antibody or antigen binding fragment thereof that binds the polypeptide encoded by the EST of interest. The kit can be in any suitable format, and in some embodiments includes instructions for use. Data Mining

[0146] Complementary DNA sequences (consisting of contigs and singletons) obtained from healthy shrimp hepatopancreas library are provided in Appendix A, and the cDNA sequences (consisting of contigs and singletons) from the white spot syndrome virus affected library in Appendix A. The unigene sets obtained by combining the sequences from the two libraries are provided in Appendix A. BLAST search analysis using the cDNA sequences from the healthy (Appendix A) or WSSV- infected (Appendix A) shrimp libraries against the combined unigene set (Appendix A) will enable to determine which genes are present only in the healthy, only WSSV- infected and both healthy and WSSV-infected shrimp. These genes may represent unique for the health status (healthy or virus infected) of shrimp.

Examples

[0147] The invention, as contemplated herein, is described in the following examples for exemplification purposes only and is not intended to limit the scope of the invention.

Example 1. Isolation of expressed sequence tags (ESTs).

[0148] ESTs have been isolated from cDNA libraries of healthy and WSSV-infected shrimp hepatopancreas tissue. Two cDNA libraries were constructed from hepatopancreas tissues of healthy (PvH) and WSSV-infected (PvW) P. vannamei shrimp using SMART cDNA amplification method (BD Bioscience, California) and cloned into a plasmid vector (pAL16, Evrogen, Inc., Moscow, Russia). Randomly selected recombinant clones were sequenced using vector-derived primers (SP6 and T7). A total of 1248 clones from PvH and 1152 clones from PvW library were sequenced.

[0149] Complementary DNA sequences from both libraries were edited and assembled using the programs Phred, CodonCode Aligner and Phrap. Vector sequences were removed from the sequence of each clone and ESTs having Phred score of >20 were taken for BLAST search. A total of 1248 clones from PvH and 1152 clones from PvW library were sequenced. Seventy five percent of the PvH clones (940 out of 1248 PvH clones; Phred score>20, average length 473 bases) and seventy three percent of the PvH clones (840 out of 1152 PvW clones; Phred score>20, average length 440 bases) were taken for further analysis. PvH clones represented 269 singletons and 124 contigs (each contig contains between 2-20 clones), whereas the PvW clones represented 281 singletons and 211 contigs (each contig contains 2-20 clones). Among the PvH clones, 38.7% of the singletons (105/269) and 71.4% of the contigs (90/ 124), showed significant similarity to known genes from other organisms by BLAST search (e>10^"3). For the PvW clones, 44.0% of the singletons (123/281) and 54.5% of the contigs (115/211) showed similarity to GenBank entries by BLAST search (e>10^'3). The remainder of the clones from both libraries showed no similarity to the database entries, and thus may represent novel genes. Combining the ESTs from healthy and WSSV- infected shrimp cDNA libraries, there were a total of 683 unigenes (Figure 3). [0150] The average GC content of the PvH clones was 0.427±0.005 and for the PvW clones was 0.424±0.005. Based on gene homology, the PvH and PvW unigene sets were categorized into the following nine functional classes following published protocol (Adams et al., 1995): Cell division, cell signaling/ communication, cell structure, cell defense, gene/protein expression, metabolism, ribosomal proteins, unknown genes and no matches (Fig. 1, Table 1). PvW library contained a significantly higher number of cell defense genes compared to the PvH library (19.4% vs. 8.1 %), whereas the PvH library contained a significantly higher number of metabolic genes (37.5% vs. 25.6%) (Fig.l). A total of 40 immune genes were identified from both libraries (Table 2). A list of these immune genes, along with their frequencies in the healthy and WSSV-infected libraries and similarities with the GenBank database entries, is provided in Table 2. The expression profile indicates a differential expression of these genes in healthy and WSSV-infected shrimp. Table 1. Functional classes of genes isolated from hepatopancreas cDNA libraries of healthy and WSSV infected shrimp (P. vannamei) that showed homology with existing GenBank database entries.

Table 2. Summary of immune genes isolated from healthy and WSS V-infected shrimp hepatopancreas cDNA libraries.

Table 2. (Cont) Right hand columns of the above section of Table 2. Correspond line for line to the other section.

Example 2. Confirmation of differential expression of shrimp ESTs.

[0151] As a proof of principle, the differential expressions of three shrimp ESTs were confirmed by examining their mRNA expression in healthy and WSSV-infected shrimp by real-time RT-PCR assay. These genes include a λ interferon inducible lysosomal thiol reductase (GILT) gene, a lectin gene named P-selectin, and a chitinase gene.

[0152J The predicted amino acid sequences of the shrimp λ interferon inducible lysosomal thiol reductase (GILT) gene, a lectin gene named P-selectin, and a chitinase gene and their similarity with the GenBank database entries are given below (Tables 3- 5).

Table 3. The similarity between shrimp λ interferon inducible lysosomal thiol reductase (GILT) gene and homologous genes in the GenBank database.

Table 4. The similarity between the shrimp chitinase gene and homologous genes in the GenBank database.

Table 5. The similarity between shrimp P-selectin gene and the homologous genes in the GenBank database.

[0153] In order to measure the mRNA expression of these three genes in healthy and WSSV-infected shrimp, primers were designed using Primer Express software (Applied Biosystem, Inc.). The list of primers is given below (Table 6). Table 6. List of primers used for measuring the expression of immune genes in healthy and white spot syndrome virus (WSSV) infected shrimp (JPenaeus vannamel) by real-time RT-PCR.

[0154] The real-time RT-PCR assay showed that shrimp λ interferon inducible lysosomal thiol reductase (GILT) , P-selectin, and the chitinase genes showed differential expression between healthy and WSSV-infected shrimp. The mRNA expression of λ interferon inducible lysosomal thiol reductase (GILT) gene was down- regulated, where as the expression of chitinase and P-selectin was up-regulated in WSSV-infected shrimp. The real-time PCR assay was performed using 5 healthy and 5 WSSV-infected laboratory challenged shrimp. The summary of the real-time RT-PCR assay for each of the three genes is provided below (Tables 7-9).

Table 7. Relative quantification of λ interferon inducible lysosomal thiol reductase (GILT) mRNA expression in healthy and white spot syndrome virus-infected shrimp (JPenaeus vannamei) by real-time RT- PCR.

Fold

Sample Average ΔCT ± Sample Average Changes

# SD # Average ΔCT ± SD ΔΔCT

H1 5.533 ± 0.267 W1 6.333 ± 2.067 -2.507 -5.7

H2 6.100 ± 0.300 W2 10.133 ± 1.733

H3 5.600 ± 0.200 W3 8.067 0.333

H4 5.767 ± 0.033 W4 9.933 1.533

H5 6.233 ± 0.433 W5 7.300 ± 1.100

Hl to H5 represents healthy animals, and Wl to W5 represents WSSV-infected animals. CT represents threshold PCR cycle number. ΔCT represents the normalized Ct value. Normalized Ct value is obtained by subtracting the CT value of RT-PCR internal control gene, shrimp elongation factor- 1 alpha, from the CT value of the target gene. ΔΔCT obtained by subtracting the ΔCT of healthy from the ΔCT value for the WSSV-infected sample. Fold changes indicates the changes in target gene expression in the WSSV-infected compared to the healthy animals.

Table 8. Relative quantification of chitinase mRNA expression in healthy and white spot syndrome virus-infected shrimp {Penaeus vannamei) by real-time RT-PCR.

Sample # Average ΔCT ± SD Sample # Average ΔCT ± SD Average Fold Changes

ΔΔCT /2-kΔCTv

H1 1.100 ± 0.040 W1 -0.300 ± 0.740 0.700 1.62

H2 1.167 ± 0.027 W2 0.967 ± 0.967

H3 1.200 ± 0.060 W3 0.033 ± 0.407

H4 0.367 ± 0.773 W4 1.267 ± 1.227

H5 1.900 ± 0.760 W5 0.233 ± 0.260

Table 9. Relative quantification of P-selectin mRNA expression in healthy and white spot syndrome virus-infected shrimp {Penaeus vannamei) by real-time RT-

PCR.

Sample # Average ΔCT ± SD Sample # Average ΔCT ± SD ΔΔCT Fold Changes

H1 3.967 ± 0.733 W1 3.567 ± 0.033 1.100 2.11

H2 4.667 ± 0.033 W2 4.700 ± 1.100

H3 5.167 ± 0.467 W3 2.100 ± 1.500

H4 4.267 ± 0.433 W4 4.133 ± 0.533

H5 5.433 + 0.733 W5 3.533 ± 0.067

Hl to H5 represents healthy animals, and Wl to W5 represents WSSV-infected animals. CT represents threshold PCR cycle number. ΔCT represents the normalized Ct value. Normalized Ct value is obtained by subtracting the CT value of RT-PCR internal control gene, shrimp elongation factor- 1 alpha, from the CT value of the target gene. ΔΔCT obtained by subtracting the ΔCT of healthy from the ΔCT value for the WSSV-infected sample. Fold changes indicates the changes in target gene expression in the WSSV-infected compared to the healthy animals. Example 3. Cloning of shrimp ESTs.

[0155] In order to amplify the full-length open reading frame (ORF) in shrimp ESTs, primers will be designed using primer express software (Applied Biosystems, Inc.). Primers will also be designed to amplify a truncated version of the gene (representing the functional domain only). Amplified cDNA will be cloned into bacterial expression vector (such as pETlOO Directional TOPO Expression vector, Invitrogen Inc.) and used to transform BL 21 Star (DE3) One Shot Chemically Competent Escherichia coli (Invitrogen, Inc.) following manufacturer's protocol.

Example 4. Bacterial expression of shrimp recombinant or isolated protein and formulation into feed.

[0156] The expression of the recombinant protein will be either constitutive or under an induction system (e.g., induced by IPTG) and empirically optimized to obtain maximum protein expression. Escherichia coli strain BL21 cells carrying IPTG inducible gene will be grown in LB medium containing ampicillin, and then will be induced with IPTG for the expression of recombinant protein. Bacterial biomass containing cells, which express the shrimp recombinant protein, will be added to shrimp feed in a free or microbound format (e.g., in beads composed of alginate and starch in a polymeric form). Attractants are added to make the feed or beads more palatable to the target species (in the case of shrimp, krill meal would be a good attractant).

Example 5. Method for protection of shrimp from WSSV infection.

[0157] Shrimp are fed either a control diet or a diet containing bacterial biomass expressing recombinant protein (Example 4). Animals are challenged with WSSV₅ and their survivability in response to viral infection will be measured. The WSSV load in the control and treatment samples is measured by real-time PCR following published protocol (Dhar et al. 2001). The mRNA expression of the target gene will be measured in the treated and control animals using real-time RT-PCR to determine the difference in expression in two treatment groups following the published method (Dhar et al. 2003). Protection from viral challenge will be determined by an increased survival versus a control not fed the diet containing the WSSV recombinant protein. Example 6. DNA method for protection of shrimp from WSSV infection.

[0158] Shrimp are immersed in a medium containing nucleic acid encapsulated in a liposome. The DNA will be designed to express proteins that are upregulated during infection by WSSV as determined from the differential EST libraries. The DNA is then absorbed through the gills or through gut lining and will then expressing the proteins in the tissues that absorb the DNA and thus providing protection against WSSV.

Example 7. RNAi method for protection of shrimp from WSSV infection.

[0159] A portion of a gene or genes identified using a differential expression library from shrimp (healthy versus WSSV-infected) as described in the specification are used to model a small interfering RNA as described by Timmons et al (2001). This DNA is then fed to the shrimp via incorporation in a feed or feed supplement. As shown for C. elegans in previous research (Timmons et al. 2001), this can interfere with expression of specific genes. Genes that are up-regulated in the EST differential library that have no defense function maybe important to viral infection and will be down regulated using this RNAi and this will suppress or inhibit WSSV infection.

Example 8. Diagnostic for WSSV infection.

[0160] The differentially expressed genes that are tied directly to WSSV infection, or general viral infection, will be used in a multiplexed assay to determine the metabolic health of a cultured shrimp population. Compared to WSSV titer determination, which tell the relative amount of virus in the population, this diagnostic will determine the effect of the infection on the metabolic health of the population (progress of the disease compared to the growth of the pathogen). Metabolic genes will be chosen from the EST library that are most influenced in the early, mid, and late stage infection. Defense genes will be chosen from the EST libraries that are the best indicators of early, mid, and late stage infection. These genes will be synthesized with a fluorescent tag on one end and a quencher on the other end as previously described for the molecular beacon system (Cantor 1996; Tyagi and Kramer 1996; Little and Vonk 2000; Livak et al 2000; Tyagi et al. 2000). These will be printed on a chip and then a crude DNA preparation from the infected shrimp used for hybridization of the chip. The detection will be carried out on a chip reader and monitored for the appearance of fluorescence and quantity of fluorescence relative to total DNA. This will be correlated empirically to disease progression and used as a way to monitor the disease status of the animals. This will be particularly important as disease resistant lines of shrimp are developed.

Example 9. Method for protection of shrimp from Taura syndrome virus (TSV") infection.

[0161] ESTs isolated from the healthy and WSSV-infected shrimp can be used for developing therapeutics for not only WSSV but also for other viral diseases such as Taura syndrome disease caused by the Taura syndrome virus (TSV). Taura disease, caused by the TSV, has caused catastrophic losses in the Western Hemisphere, and more recently in Taiwan (Dhar et al , 2004). The cDNA libraries described in Example 1 contained immune genes such as the low-density lipoprotein receptor (LDLr) gene (Table 2) that could be for developing therapeutics against the TSV. In addition to binding and internalizing lipoprotein molecules from circulating haemolymph, LDLr is known to be involved in viral pathogenesis. For example, Hepatitis C virus has been shown to complex with LDL and interacts with the LDL receptor (Wunschmann et al. , 2000). Type 2 rhinovirus was shown to up-regulate LDL receptor expression on human tracheal epithelial cells (Suzuki etal, 2001) and binding of the HIV-transactivator protein is mediated by LDL-related protein (Liu et al., 2000).

[0162] Primers will be designed to amplify the full-length open reading frame (ORF) of shrimp LDLr using primer express software (Applied Biosystems, Inc.).

Primers will also be designed to amplify a truncated version of the gene (representing the functional domain only). Amplified cDNA will be cloned into bacterial expression vector (such as pETlOO Directional TOPO Expression vector, Invitrogen Inc.) and used to transform BL 21 Star (DE3) One Shot Chemically Competent Escherichia coli (Invitrogen, Inc.) following manufacturer's protocol. Escherichia coli strain BL21 cells carrying IPTG inducible gene will be grown in LB medium containing ampicillin, and then will be induced with IPTG for the expression of recombinant protein. The expression of the recombinant protein will be empirically optimized to obtain maximum induction. Bacterial biomass containing cells, which express the shrimp recombinant protein, will be added to shrimp feed in a free or microbound format (in beads composed of alginate and starch in a polymeric form). Attractants are added to make the feed or beads more palatable to the target species (in the case of shrimp, krill meal would be a good attractant). [0163] Animals will be challenged with TSV, and their survivability in response to viral infection will be measured. The TSV load in the control and treatment samples is measured by real-time PCR following published protocol (Dhar et al. 2002). The mRNA expression of the target gene will be measured in the treated and control animals using real-time RT-PCR to determine the difference in expression in two treatment groups following published method (Dhar et al. 2003). Protection from viral challenge will be determined by an increased survival versus a control not fed the diet containing the TSV recombinant protein.

Example 10. Use of recombinant lectins for controlling viral and bacterial diseases of shrimp and other aquaculture species.

[0164] The differentially expressed genes in shrimp included a number of lectin genes (see Table 2, Gene ID# PvH09A06, PvHl 3 C04, and PvW04E03. Primers will be designed to amplify the full-length open reading frame (ORF) of shrimp lectins using primer express software (Applied Biosystems, Inc.). Primers will also be designed to amplify a truncated version of the gene (representing the functional domain only). Amplified cDNA will be cloned into bacterial expression vector (such as pETlOO Directional TOPO Expression vector, Invitrogen Inc.) and used to transform BL 21 Star (DE3) One Shot Chemically Competent Escherichia coli (Invitrogen, Inc.) following manufacturer's protocol. Escherichia coli strain BL21 cells carrying IPTG inducible gene will be grown in LB medium containing ampicillin, and then will be induced with IPTG for the expression of recombinant protein. The expression of the recombinant protein will be empirically optimized to obtain maximum induction. Bacterial biomass containing cells, which express the shrimp recombinant protein, will be added to shrimp feed in a free or microbound format (in beads composed of alginate and starch in a polymeric form). Attractants are added to make the feed or beads more palatable to the target species (in the case of shrimp, krill meal would be a good attractant).

[0165] Animals will be challenged with WSSV, TSV, yellohead virus (YHV), and infectious hypodermal and hematopoietic virus (IHHNV) and bacterial pathogens such as Vibrio sp. including V. penaeicida, an important bacterial pathogen of shrimp. The survivability of shrimp after viral and bacterial challenge will be recorded. The viral load (WSSV, TSV, YHV and IHHNV) will be measured by real-time PCR following published protocol (Dhar et άl, 2001, 2002). The mRNA expression of the lectin gene in the virus and bacterial-challenged animals will be measured in the treated and control animals using real-time RT-PCR to determine the difference in expression in two treatment groups following published method (Dhar et al. 2003). Protection from viral challenge will be determined by an increased survival versus a control not fed the diet containing recombinant lectin. [0166] In addition, by using homology modeling the tertiary structure of shrimp lectin genes will be determined. Shrimp lectins mentioned in see Table 2, Gene ID# PvH09A06, PvH13C04, and PvW04E03 will be taken for determining the tertiary structure by comparing with the crystal structure of homologous lectin available in the GenBank database (Feinberg et al., 2001). Homology modeling will enable to determine the sugar binding pocket. Ligand binding assay ,by virtual docking of different ligands (sugars), will be performed, and the affinity of different lectins to different sugars will be determined. Based on sugar binding affinity, recombinant lectins could be used as feed supplement to protect shrimp and other aquaculture species from different viral and bacterial disease.

Example 11. Method for protection of shrimp and other aquaculture species from bacterial diseases using recombinant anti-lipopolysaccharide protein.

[0167] Analysis of shrimp EST sequence revealed the presence of an anti- lipopolysaccharide (Anti-LPS) gene (see Table 2 EST ID #PvHBl 1). Anti-LPS gene encodes for small a basic protein that binds and neutralizes LPS, and thus possesses a strong antibacterial effect on the growth of Gram-negative bacteria (Iwanaga et al., 1992; Ried et al., 1996). Primers will be designed to amplify the full-length open reading frame (ORF) of shrimp anti-LPS gene using primer express software (Applied Biosystems, Inc.). Primers will also be designed to amplify a truncated version of the gene (representing the functional domain only). Amplified cDNA will be cloned into bacterial expression vector (such as pETlOO Directional TOPO Expression vector, Invitrogen Inc.) and used to transform BL 21 Star (DE3) One Shot Chemically Competent Escherichia coli (Invitrogen, Inc.) following manufacturer's protocol. Escherichia coli strain BL21 cells carrying IPTG .inducible gene will be grown in LB medium containing ampicillin, and then will be induced with IPTG for the expression of recombinant protein. The expression of the recombinant protein will be empirically optimized to obtain maximum induction. Anti-LPS recombinant protein will be purified from the recombinant cells and its Gram-negative bacterial neutralizing activity will be assayed against a number of Gram-negative bacterial pathogens of shrimp, fish and terrestrial agricultural species e. g. Vibrio penaeicida, Vibrio anguillarium, Virio parahemolyticus, Lactococcus garviae, Pasteur ella piscicida.

[0168] Bacterial biomass containing cells, which express the shrimp recombinant anti-LPS protein, will be added to shrimp feed in a free or microbound format (in beads composed of alginate and starch in a polymeric form). Attractants are added to make the feed or beads more palatable to the target species (in the case of shrimp, krill meal would be a good attractant). Shrimp will be challenges with the bacterial pathogen and mortality will be compared to control treatment where the animals will be fed pellet without containing any anti-LPS recombinant protein.

Example 12. Confirmation of differential expression of shrimp ESTs

[0169] As a proof of principle, the differential expression of nine more ESTs in healthy and WSSV -infected shrimp were evaluated by real-time RT-PCR assay. These include Interleukin enhancer-binding factor 3, Tetraspanin-2, NF-kappaB essential modulator, Prophenoloxidase-activating proteinase 2, T-cell activation protein, O- sialoglycoprotein endopeptidase, Ubiquitin, Anti-lipopolysaccharide Factor, Lysozyme 2, and Heat-shock protein (Table 10 and 11; Fig. 3). Out of these nine genes, four (Tetraspanin-2, T-cell activation protein, Anti-lipopolysaccharide Factor, and Heat- shock protein) genes were up-regulated and five genes (Interleukin enhancer-binding factor 3, NF-kappaB essential modulator, Prophenoloxidase-activating proteinase 2, O- sialoglycoprotein endopeptidase, Ubiquitin, and Lysozyme 2) were down-regulated during WSSV infection in shrimp (Table 11, Fig. 3).

Confirmation of differential expression of shrimp EST in Taura syndrome virus infected shrimp

[0170] Taura syndrome (TS) disease, caused by the Taura syndrome virus (TSV), pose a major threat to shrimp mariculture in both hemispheres. Although considerable progress has been made in elucidating the organization of the TSV genome and developing TSV-specific diagnostic methods, information on shrimp cellular genes involved in TSV pathogenesis and cellular immunity remains unknown.

[0171] Two separate strains of P. vannamei juveniles (one TSV-resistant (SPR) and one Fast Growth (FG) TS V-susceptible line) were per os exposed (Texas 2004 TSV strain). Moribund acutely infected shrimp, surviving chronically infected shrimp and unexposed negative control shrimp were preserved in Davidson's (AFA) for histological analysis. Mild to moderate multifocal pathodiagnostic acute phase epithelial necrosis was detected in moribund FG shrimp, which suffered 20-36% mortality. Moderate to severe lymphoid organ spheroids were detected in chronically infected FG survivors. No mortality or acute TSV lesions were detected among the 5 TSV-exposed SPR groups.

[0172] A cDNA clone from a healthy Penaeus vannamei (clone ID#PvH04G08) showed similarity with the low-density lipoprotein receptor (LDLr) gene of human, mouse, Drosophila and Caenorhabditis elegans. The LDLr gene is a member of an evolutionarily conserved family of multifunctional receptors that binds to rhinoviruses 0 (Family Picornaviridae) and a variety of ligands. Upon binding to the ligands, LDLr transports the macromolecules through receptor- mediated endocytosis. The LDLr gene expression was measured in both healthy and TSV-infected (acute and chronically infected) shrimp by real-time RT-PCR. LDLr mRNA expression was almost 4-fold higher in the healthy TSV-resistant SPR shrimp compared to the healthy FG TSV- 5 susceptible line. In the SPR animals, LDLr expression increased upon TSV challenge (3.3 to 6.6-fold higher expression depending on the TSV load). In the FG TSV- susceptible acute phase animals, there was no increase in the LDLr expression. However, in the FG TSV-susceptible chronic phase animals, LDLr expression was 5- fold lower compared to the healthy control animals. These data indicate that LDLr 0 expression is differentially modulated in the TSV-resistant and susceptible animals. Therefore, LDLr gene could be used as a potential target for developing therapeutics against Taura syndrome disease in shrimp. In addition, SPR animals showed higher expression of LDLr compared to the TSV-susceptible line making LDLr gene as a candidate genetic marker for marker-assisted selection in developing TSV-resistant line 5 of shrimp.

Table 10. List of primers used for measuring the expression of immune genes in healthy and white spot syndrome virus (WSSV) infected shrimp {Penaeus vannamei) by real-time RT-PCR.

Table 11. Relative quantification of differentially expressed immune genes in healthy and white spot virus infected shrimp (P. vannameϊ) by SYBR Green real-time RT-PCR

Shrimp Gene Average ; Δ CT value Average ; Δ CT value in ΔΔCT Fold Changes

Clone ID# in healthy shrimp ± WSSV infected shrimp

(Primer SD ± SD in WSSV-

Name) infected shrimp

Gamma interferon inducible PvHlA02 lysosomal thiol reductase (GILT) 5.847 ± 0.247 8.353 ± 1.353 -2.507 -5.7

PvW8B06

Interleukin enhancer-binding factor 3 7.093 + 0.392 7.120 ± 0.491 -0.027 -1.0

PvWl 1A5

Tetraspanin-2 6.320 ± 0.336 5.867 ± 0.572 +0.460 +1.4

PvW8E09

NF-kappaB essential modulator 13.780 + 0.734 13.883 + 1.114 -0.103 -1.1 'Jl

PvW9Ell

Chitinase 1.2474 + 0.440 0.440 + 0.720 +0.807 +1.8

Prophenoloxidase-activating PvW4F07 proteinase 2 13.320 ± 0.291 14.127 + 0.683 -0.807 -1.8

PvW4E03

P-selectin precursor 4.700 ± 0.480 3.610 + 0.647 +1.090 +2.1

PvW5G04

T-cell activation protein 12.587 ± 0.280 11.925 ± 0.375 +0.662 +1.6

PvHCOδ

O-sialoglycoprotein endopeptidase 12.800 ± 1.723 14.300 + 0.000 -1.500 -2.8

PvW04C06

Ubiquitin 3.123 + -0.202 3.130 ± -0.016 -0.007 -1.0

PvHBI l

Anti-lipopolysaccharide Factor 13.622 ± 0.000 7.547 ± 0.000 +6.075 +67.4

PvW10F4

Lysozyme 2 2.883 ± 0.390 3.157 ± -0.048 -0.274 -1.2

PvW10D06

Heat-shock protein 9.230 ± 0.550 8.470 + 0.002 +0.757 +1.7

Example 13. Shrimp Toll-like receptor.

This invention describes a Toll-like receptor (TLR) in shrimp challenged with WSSV that has not yet been described. TLRs are type I transmembrane proteins that serve as a key part of the innate immune system, the primary protective mechanism in shrimp. In vertebrates, they also stimulate activation of the adaptive immune system, linking innate and acquired immune responses. TLRs are considered pattern recognition receptors (PRRs), binding to pathogen-associated molecular patterns (PAMPs), small molecular sequences consistently found on pathogens. Their function is the recognition of pathogens and the activation of immune cell responses directed against those pathogens. TLRs were first discovered in the fruit fly Drosophila melanogaster. TLRs are present in mammalian immune cells as well as in numerous other animals (including human, mice, goldfish and chickens). They have even been found in plants and are thus believed to have an ancient evolutionary origin; after the defensins, they may be the oldest components of the immune system. This is the first description of a TLR in shrimp.

In vertebrates, innate immunity provides a rapid response to invasive stimuli (e.g. bacteria) mediated through pattern-recognition receptors such as the toll-like receptors (TLRs). These TLRs are critical signalling proteins for bacterial lipopolysaccharides, bacterial LPS/lipoproteins and bacterial DNA. Other members of the same receptor superfamily are involved in the regulation of ThI /2 T-cell function. Stimulation of TLR signalling pathways in tissues result in the rapid generation of an inflammatory response and the production of proinflammatory cytokines, such as IL-I, tumor necrosis factor-w and the chemokines. Leukocytes also express TLRs, which be involved in the regulation of their function. Signalling by LPS, perhaps predominantly mediated via TLR4, causes increased lifespan of neutrophils, possible upregulation of basophil histamine release, alterations in the expression of chemokine receptors on the surface of neutrophils and monocytes (which may modify processes of allergen sensitization), and chemokine generation from multiple cell types, including leukocytes and endothelial cells. Endotoxin responses may play an important role in the establishment of allergen sensitization in asthma, with variations in the LPS co-receptor CD 14 having functional significance in the development of atopy (together with the current hygiene hypothesis theories), and there is preliminary animal evidence that defects in TLR4 signalling can modify sensitization (Sabroe et al. 2002).

Invertebrates are known to rely on innate immunity, since they have not developed an elaborate acquired immunity (Arala-Chaves and Sequeirab 2000; Bachere 2000; Smith et al. 2003; Liu et al. 2004). This invention's identification of a putative TLR provides a key to understanding the shrimp innate immune response and development of drugs targeted to either enhance the effect, therefore stimulate a rapid innate immune protection, or to inhibit the effect, preventing a drain on the production of the organism for diseases such as the currently prevailing strains of a shrimp virus, infectious hypodermal and hematopoietic necrosis virus, IHHNV that do not cause mortality but long-term chronic infections.

Shrimp TLR cDNA clone represents partial sequence of the full-length gene. The sequence is used to design primers for cloning the 5'-and the 3'-end of the gene using standard 5'- and 3'- rapid amplification of cDNA ends method (RACE, such as First Choice RLM-RACR kit sold by Ambion, texas, (Sambrook et al. 1989; Jurecic et al. 1998)). This will allow the recovery of the complete gene from total mRNA. The full-length shrimp TLR gene is then cloned into a bacterial expression vector such as pET28a+ (Novagen) using the manufacturer's instructions. The protein is then expressed during log phase growth under induction with IPTG (an inducer of the lac operon promoter). Proteins are purified using nickel columns that react with the his- tag labeled recombinant proteins expressed in this method using standard methods (Sambrook et al. 1989).

The recombinant, tagged fusion protein is then used as a tool to investigate binding to either combinatorial libraries or to natural product libraries to determine if compounds that specifically bind this receptor are available. Such libraries are available at large pharmaceutical companies and have been previously described. Once receptor-binding chemicals are identified, in vivo testing can be attempted using antibodies made to the TLRs identified in this invention. Such antibodies can be made as either polyclonals (to peptide or whole protein) or monoclonals by a number of different contract laboratories by standard methods (e.g., Sigma Genosys, Spring Valley Labs, BioResource Intl). Shrimp are treated with the TLR-binding chemical and then humanely sacrificed. Tissues are extracted for membrane proteins and western analysis, using the TLR specific antibody, is performed using standard methods (Sambrook et al. 1989). Compounds that up-regulate or down-regulate the TLR receptor are identified by this method.

Shrimp are then dosed with compounds that up-regulate or down-regulate the TLR to determine concentrations that do not affect the health of the shrimp. One or two dosing regimes, identified in this manner, are then used to treat the shrimp in an experimental system. The shrimp are then challenged with WSSV via oral delivery of live virus in the form of infected shrimp tissues. The mortality of the shrimp is monitored to determine which drugs provide protection from WSSV infection.

Example 14 - POC Diagnostic based on TLR

Rapid identification of disease is essential to the well being of the animals as well as the cost effectiveness of aquaculture. Using antibodies designed in Example 1, one is able to design a point-of-care diagnostic that will allow rapid detection of the immune status of the. shrimp. This is done by immobilization of one of the anti-TLR antibodies described in Example 1 on activated paper used for protein immobilization in POC diagnostics (e.g., Whatman's Purabind membranes). This paper is then blocked with a protein-containing buffer (2% bovine serum albumin) to fill any remaining protein binding positions and dried. The dried paper is now a capture surface for the shrimp TLR protein. An absorbent pad is placed at one end such as CF6 (Whatman) to act as a sink for liquids wicking across in a lateral flow assay. A representative shrimp is removed from the pond and humanely sacrificed. The tail muscle is macerated in pH 7.0 buffer containing a detergent such as tween-20. The extracted muscle is clarified by centrifugation or placed directly on one end of the dried paper. The clarified liquid is pulled by capillary action over the immobilized anti-TLR and any TLR is captured. Excess fluid is removed by the wick at the end of the paper. At this point, dye labeled anti-TLR in a buffer containing 1% bovine serum albumin is loaded on the end of the paper and wicked across the immobilized TLR by capillary action. If high levels of TLR are present in the tissues, dye will accumulate on the line and the immune status of the animal will be evident.

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Lung Cell MoI Physiol. 2001. 280:L409-L420. Timmons L, Court DL, Fire A (2001) Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans. Gene 263:103-112 Tsai MF et al. (2000a) Transcriptional analysis of the ribonucleotide reductase genes of shrimp white spot syndrome virus. Virology

277:92-99. Tsai MF et al. (2000b) Identification and characterization of a shrimp white spot syndrome virus (WSSV) gene that encodes a novel chimeric polypeptide of cellular-type thymidine kinase and thymidylate kinase. Virology 277:100-110. Tyagi S, Kramer FR (1996) Molecular beacons: Probes that fluoresce upon hybridization. Nature Biotechnology 14:303-308 Tyagi S, Kramer FR, Lizardi PM (2000) Detectably labeled, dual conformation oligo-nucleotide probes, assays and kits. In: US

Pat. No. 06103476. P ATENT NUMBER- 06103476 van Hulten MC, Goldbach RW, Vlak JM (2000a) Three functionally diverged major structural proteins of white spot syndrome virus evolved by gene duplication. J Gen Virol 81:2525-2529. van Hulten MC, Westenberg M, Goodall SD, Vlak JM (2000b)

Identification of two major virion protein genes of white spot syndrome virus of shrimp. Virology 266:227-236. van Hulten MC et al. (2001) The white spot syndrome virus DNA genome sequence. Virology 286:7-22. Vasta GR, Quesenberry M, Ahmed H, O'Leary N (1999) C-type lectins and galactins mediate innate and adaptive immune functions: their roles in the complement activation pathway. Dev Comp

Immunol 23: 401-420 Vorup- Jensen T, Petersen SV, Hansen AG, Poulsen K, Schwaeble W,

Sim RB, Reid KBM, Davis SJ, Thiel S, Jensenius JC (2000)

Distinct pathway of mannan-binding lectin (MBL)- and Cl- complex autoactivation revealed by reconstitution of MBL with recombinant MBL-associated serine protease-2. J Immunol 165:

2093-2100 Wunschmann, S., Medh, J. D., Klinzmann, D., Warren, S. N., and

Stapleton, J. T. Characterization of Hepatitis C Virus (HCV) and

HCV E2 interactions with CD81 and the low-density lipoprotein receptor. Journal of Virology. 2000. 74:10055-10062. Yang F et al. (2001) Complete genome sequence of the shrimp white spot bacilliform virus. J Virol 75 : 11811 - 11820. Yi G, Qian J, Wang Z, Qi Y (2003) A phage-displayed peptide can inhibit infection by white spot syndrome virus of shrimp. J Gen

Virol 84:2545-2553. Zhang X, Xu X, Hew CL (2001) The structure and function of a gene encoding a basic peptide from prawn white spot syndrome virus.

Virus Res 79:137-144. [0173] The above examples are included for illustrative purposes only and is not intended to limit the scope of the invention. Since modifications will be apparent to those of skill in this art, it is intended that this invention be limited only by the scope of the appended claims.

[0174] Appendix A

Unigeπe healthy SSH library (contigs+singletons)

>HP 14_SSH_trimmed.fasta.Contig 1

TGAGCTCAAGCAGTGGTACACGCATGTACGCCCTCGAGCGGCGCCAGGCA

GGTACCCAACCTTCCTCCTCCCGGATTGACATGTGGCTTCTCGACCCCTG

CGCGGATCTCGGACTCTCTCTGGCAACTGACTCTTGCCAGCCCACCCCTC

CTCCCTCCCTGTCAGCCAGCGGGAGCAAACGTCAGCCGAACATCGTTCCT

GTCAGCACTGCAAATATCCCTCTGCCCTTTCATGCAGCTTTTCCTATCTG

GGCGCGAACCGAAAGAGATCATCTCACCTTTAATTCTCGTGATCATTTTA

TTGTGAAACTCACGAAAGCTAAATTGCATCTTTGTGTATCAATTTACTCA

TCATGATGAATATGTCGCTTGTATCAAGCATTCATTAATTGTTCAGTTCA

CACACTCTCAGTCTGCCATGCTTCTGGATGACGTGGCATTCGACTATACA

TTGTTCATTTGATGTAITTGTGATATTTCTTGATCAAACTTTTCCTCATT

GCTCAGAGCACTTTGTTGTATTTCAAGTACCTCGGCCGCGACCACGCTGG

GCGTACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCC

TTTAGTGAGGGTTAATTGCGCXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGNCCACTTGA

ATCNTGGTCGGTGTGTCTCTCTGCTTTTCGCTGCGCTGGTTCTCCG

>HP 14_SSH_trimmed.fasta.Contig2

TGAGCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGCCGA

GGTACAGAAACTTGTTAGAGACCTTAAGGATCACAGACTTCTCGAACAGA

AGCACTGGTTTTCTCTCTTTAACCCTACTTCCGTCAGCATATGAATGAAG

GAGAGTTTGTCTATGCCTTGTATGTTGCGGTCATCCACTCACCTCTGGCT

GAGCACGTTGTGCTCCCTCCACTCTATGAGGTCACACCTCATCTCTTCAC

TAACAGCGAAGTTATTGAATCAGCTTATCGTGCCAAGCAGACACAAAAAC

CTGGTAAATTTAAGTCTAGTTTCACTGGAACTAAAAAGAATCCTGAACAG

AGAGTAGCCTATTTCGGAGAGGACATTGGAATGAACACTCACCATGTTAC

CTGGCATATGGAATTCCCATTCTGGTGGCAAGACAGTTACAGTCATCACC

TGGACCGCAAAGGAGCGAACTTCTTCTGGGTACCTGCCCTGGCGGCCGCT

CGAGGGCGTACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTG

TTCCCTTTAGTGAGGGTTAATTGCGCXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXCTGGCGGCAGCNGGTTCAGTCCTCCANGGGGGGAGGTCTCC

CCCNTGCNCCGGGTCTCATCCGGGAACATCTGTTGCCGGCGCGGGGGCAA

GCACAAGG

>HP14_SSH_trimmed.fasta.Contig3 GATGGAGCTCCAAGCAGTGGTACACGCAGAGTACGCCCGCGGGGTCGCGG

CCGAGGTACATGGGATTATAGTTCAAAATAGCTTCCTATTAATGCTGGAT

ATACTCGCCATGGTTGAAGACCTTAACTTGGATGTGGCCGAAGTTGGGAA

GATCTTCGAATACACGCTCATCGGGAACCTTACGATCCAGAGGGTAGCCA

TGTGGGCGATTGTCAGGGTATTTGCCATGAGCACCATAGTGGTTGAATTC

GGTATTTTCGTGGAGGCCATCCAATGCTGCGTCGGCTGCACCATCAGTCA

CAGCTACAACAAGGTCGAACTCCAGACCCTGTTCATTACCCTTGGGGAGC

AGGAATCGATTTGGAATACCAGTGGCACTCCCGAAATCTGCAAGTCCGGA

GTCTGCACCACCTAAGGCTTCTTTGGTCTTCTCAAAGAGTGTTGCAAAAC

TAGGCACATCAGGCACAGTAACAGCAGATTCCCCCGCGTACCTGCCCTGG

CGGCCGCTCGAGGGCGTACTTCTGTCGTCTTGAGCGTACTGATGGTACCC

AGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGGTTTCTTCGTNCATT

GGANTCGTTGCNCGGCGTTCGGTTGGGGCAGCGGTTTGTCACTCAAGGGG

CACGGGTTCCCACCGGGTNTCCCNNNNCTNCTCG

>HP14_SSH_trimmed.fasta.Contig4

GATGGAGCCAAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGG

CCGAGGTACCACCTGAGAGCATGTGCATACTTTCCAGCTGGGGCCTTGCC

CAGAGCTTCAAACACTGCTACATCATCCTGGGAGGCAACAAACCCTTCGA

TGTAACTTTTGTCCCCAAGATAATCATTTAGGGCCTTGACGCCAGCGTCA

CTTTCCAAGTCACCGAAATTTGGCATTTTCCTGGCTTTAAGCGTCCACCC

GCGTACCTGCCCTGGCGGCCGCTCGAGGGCGTACTTCTGTCGTCTTGAGC

GTACTGATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

XXXXXXXKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGGGTTGCTGGTT

CGNCTGCGTAGCGGNCCNGCCGNCTTCTCCTTCGGGAAGGGGCGGTTCTT

AACGCGGGTCTCGGTGGGTGCCCCAGGGNGNTGGACCCTCGGAGGCTGNA

TGTGTGTCCGAGNNCTTCGCGTGATAGTAGTGTGTGGCACTCTGNTNNTT

TCNTTTTCTCTCTTCC

>HP 14_SSH_trimmed.fasta.Contig5

GAGGCCAAGCAGGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGCCGAG

GTACACTAATGTTATGAAGGACTACCATTACCACGGCAGTGAATAAAAGT

TATTGCTCACTACTCTGTCACTAAATGATATCCATTGAATTGTTATTTAG

AAATGTTACCCCATCAGTCATTATAAGTACTGATGATAGATGAAACTCAG

TTCGGTATAGTAATGAATTGACTAAATCTATGTGTTTTAGAAAATAATAA

TTCCTTTCGGGAGTAAAAAAAAAAAAAAAAAAAAAGTACCTGCCCTGGCG GCCGCTCGAGGGCGTACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAG CTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCXXXXXXXXXXXXXXXXXX xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

XXXXXXXXXXXXXXXXXXXJCKXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTTTNCAGCAAGT

TCAGNTGCGAGCCGACGTTTGTCCGGTCCGGCNCNCNTGCGACTCGTCTC

GCGNCGTAGATGCGTCTCCCNGGGGGGTCTACCGNTTCCAGGTGGGTCCC

GGGCCCCCGGGTCTTCNCACAGGGCACTCGGGTNNCTCTCCTCATCTGNT

CTTCTCNTCTCTCNCN

>HP 14_SSH_trimmed.fasta.Contig6

GATGGAGCTCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCC

AGGCAGGTACTAAGATAACTTTAATAAAATTAAGAGATAGAAACGACCTG

GCTCACGCCGGTCTGAACTCAAATCATGTAAGGATTTAAGGGTCGAACAG

ACCCCTCCTTTTATAAACCTTGGCGTGGCCATTTATTAAAGGGAAAACCC

TTTAAATTTCAAACCATCGGAGGGTTCGCAAACCCCTTTCCTTGGTTCGA

ATAATTGGGACTNCTCAAAGGGAAAGATTACCGCTGTCATCCCCTTAAAA

GTTAAACCTTTAATTCTTTATGATCTTCTAAAAGGCAGGATCATTAATTT

TTCCAGAAAATTACTGTTATTAAATTAAACCTTTTAAACGGAAAACCAAG

GTTTTAACTCAATTATTATATCCCCGTTCGCCCCCAAACGCAAACAAAAT

ATTATCTAAAAATCAAGTTATACTAACAATTTATAATATAAATAACTTAT

TGTAAAGCTTTTATAGGGTCTTATCGTCCCCTTAAATTATTTAAGCCTTT

TCACCCCGCGTACCTCGGCCGCGACCACGCTGGGCGTACTTCTGTCGTCT

TGAGCGTACTGATGGTACCCAGCTTTTTGTTCCCTTTAGTGAGGGTTAAT

TGCGC

>HP 14_S SHjximmed.fasta. Contig7

GATGAGCTCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCA

GGCAGGTACAGGTCCTTACGGATGTCCACGTCGCACTTCATGATGGAGTT

GTAGGTGGTCTCGTGGATGCCGCAGGATTCCATGCCCAGGAATGAGGGCT

GGAACAGGGCCTCGGGGCAGCGGAACCTCTCGTTGCCGATGGTGAACACC

TGGCCGTCAGGGAGCTCGTAGGACTTCTCCAGCGAGGAGGAGGAAGCAGC

GGTGGTCATCTCCTGCTCGAAGTCCAGGGCCACGTAGCACAGTTTCTCCT

TGATGTCACGAACGATTTCTCGCTCGGCGGTGGTCGTGAAGGTGTAGCCA

CGCTCCGTCAGGATCTTCATCAGGTAGTCTGTGAGGTCGCGGCCGGCCAA

GTCCAGACGCAGCCCGCGTACCTCGGCCGCGACCACGCTGGGCGTACTTC

TGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTAGTGAG

GGTTAATTGCGCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTTA CGGCCGGAAGACTNTTGCCAGGNCGAGGGGTCCCTGTCNCTTCTCCTGCT TTTTTTGCTTTCCTTTTGTCGGCCCTTGAGCTTCATNCGCTCCTGTGGGA

CCCGTTTAAAGCGGGTCCCGGCAAGCTCTGCAGNCTTTACAGACTCNCCG

GGGGACTCCACCATTGTGGTATCGTGTACCTCCCNTTCCTTTACCATCTN

CTTTTGT

>HP 14_SSH_trimmed.fasta.Contig8

GATGAGCTCAAGCAGTGGTACACGCANNTAGTACGCCCTCGAGCGGCCGC

CAGGCAGGTACAGAAACTTATGAGAGACCTTAAGGATCACAGACTTCTTG

AACAGAAGCACTGGTTCTCTCTCTTCAACCCAAGACAACGTCAGGAAGCA

CTCATGCTTTTCGATGTTCTCATCCATTGCAAGGATTGGGATTCATTTGT

CAGCAACGCTGCCTACTTCCGTCAGCGTATGAATGAAGGAGAGTTTGTTT

ATGCCCTGTATGTTGCAGTCATCCACTCACCTCTGGCTGAGCACGTTGTA

CTTCCTCCACTCTATGAGGTCACACCTCATCTCTTTACTAACAGTGAAGT

CATTGAAGCAGCATATCGTGCCAAGCAGACACAGACCCCTGGTAAATTCC

AGTCTTCCTTTACTGGAACAAAGAAGAACCCTGAACAGAGAGTAGCCTAT

TTCGGAGAGGACATTGGAATGAACACTCATCACGTTACCTGGCATATGGA

ATTCCCATTCTGGTGGCAAGACAGTTACAGTCATCACCTGGACCGCAAAG

GAGAGAACTTCTTCTGGGTACCTCGGCCGCGACCACGCTGGGCGTACTTC

TGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTTGTTCCCTTTAGTTG

AGGGTTAATTGCGCGC

>HP 14_SSH_trimmed.fasta.Contig9

AATGGAGCTCAAAGCAGTGGTACACGCATTAGTACGCCCAGCGTGGTCGC

GGCCGAGGTACGCGGGAGTTGCCACTGCAGCAGCACAACGAACCGGTGCA

CAAGAGACTCTCTCCTTTCTTTCAGTATGAAGGTGACACTGGTCCTGGTC

CTGGTGGCCGTCGTCGGCCTCTCCTCCGCCTACTCCCTCGGCGACGTCTA

CGAGGAGCTCCATCGCGTCGAGAGGGACATGAGCTCAGGGACGAGCGGAA

GCACGTCCGTGGAGAGGATAGAGAAGAGAGACATCAGCACAGGGAGCACC

AGCGACAGCGCGTCTTTTGAGAGAATAGAGAAAAGGGACATCGCTGCCAC

TCACGACTTGGATCTGAGGCGCCTCAGGAGACAAGCCTCCAGCTCCGAGA

GTGAGAGCTCTGAGGAATCTTCTGAGGAATGGGCAACTGTCGCTCCTTGA

AGGACTCATGTGACGGCGCTGACCTTTGGCGAACGACCTGACCTCCATTT

GGCCCTGATGATTTGAATTAGGATATGTCGAATAGTTTTCAAAATTCTTT

AAACTGTCTAGTAAGACACGTCATTGGATAGAATTTTCAGCTTTCATGTA

GACATAATCTGCATTTTGTGTAACCAGAATTAATAAAGAGATTGACCAAA

AAAAAAAAAAAAAAAAAAAAGTACCTGCCCTGGCGGCCGCTCGAAGGGCG

TACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTGTTCCTTTAG

TGAGGGTTAATTGGCGCTTGGGTXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXNTAAGAGCCGG

>HP 14_SSH_trimmed.fasta.Contig 10

GATGGAGCCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGG

CCGAGGTACAGGAGGCCTTTACTTCAATGACCAGAAACACACGTGTGACC

TCCCTGAAAATGTTTCCTGTGAGGAGCGACGTAAAAGAAGTGAAATAGAC

CCAGTAACGGAAGAACACTACATCTTACCGGAGGAGTGTAAGGATCTGCA

AGGAATGTATGCCATTAGGGACAGACCAAGTTCGTATTACCTTTGCAGTC

ACGGCGTAGCCTTTGAAATGCGGTGTCCAGATGGCGGCGTGTTTTCAAGC

AAAGCCAAGAAATGCATCTTAAGGAAGTAAATACTAGACGAAAAAAAGAA

GAAAAGAAATGATAAGCAAAAAACAAACAAACAAACCATACTAATAAGCT

TAGATAACGTGATTTTCTGGTTCTTCCCTGGAAATGCTGATTGTAAATAA

AATGATAATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAA

AAAAAAAAAAAAAAAAAAAAAAAGTACCTGCCCTGGCGGCCGNTCNAGGG CGTACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTGGTTCCCT

TTAGTGAGGGTTAATTGGCGC

>HP14_SSH_trimmed.fasta.Contigl 1

TGGGCNCAAGCAGTGGTACAACGCAGAGTACGCCCAGCGTGGTCGCGGCC

GAGGTACATGATGCAAAGACATGGAACTAACTACTATGCTATGTCAAAAG

ATCCCAAGAATTACTATCAAGATACCCCAAAGCAACGTGGAAGCAAAGAT

CAAATCTTATATGGACAATCCTAGATACTACATTCCAGAAATGAGGAAGA

AAGGCTTACTTCCAGCTAAATTGGCAGAGAAGAAACAGAAGGCCCAAGAC

AAGAAGAAGAAGGCACAAAAAAAAAATTCAATTCCAAGAAGAAGAAGTCA

TAAAAAGGCTTTTGTGCGTGTGAATGTGTGTATGAATTTGGTATAGATAT

AAAAACTGTCAATTTACTGTGCTATTTTGTTATTTTTTGAATGTCGACAA

AAAGTCAAAATATCTTTTGTTGTTGTTTTGTTAACTGGTTAACTAAGATA

GATGTTTTATGCTTTATTCTTTATTATATAATAACAGTTGGTAAATTGCA

AAAAAAAAAAAAAAAAGTACCTGCCCTGGCGGCCGCTCGAGGGCGTACTT

CTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTAGTGA

NGGGTTAATTTGCGCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

XXXXXXXXXXXXXXXXXCTTGGGGGGTTGCCCTAATTGGAGTTGAAGCCT

TAAACTTCCACCATTATTTGCGGTTGCGCCTTCAACTGCCCGTTTCCAAG

TCCGGGGAAACCTTGTTCGTGGCCAGTTGGCCTTATGTTGAATCGGGCCA

CCGCCGCGGGGAGAGGGCGTGGCTATGGGCCGNCTTCGTNCTCCGCCTGG

ACTCGTGCGCCCTCNGTNNTTATCGTTTNNGGCACGGNTCAGTCACCAGA

GGGGGAAAG

>HP 14_SSH_trimmed.fasta.Contigl 2

GATGGAGCCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGG

CCGAGGTACTCCCTCAAGTTATCAGAGATGAAAATCAGACCAAGACAGTA

AATCCAAGAGAAGATAAGGACAGAGAAGAAAAAAAAACGAACAGCTGAGA

CACAAGAGAAATCGGAAATTACGATCATTTACAAAAGAAATACGATCTGT

GTACCTGCCCTGGCGGCCGCTCGAGGGCGTACTTCTGTCGTCTTGAGCGT

ACTGATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXCCTTACCCCCTGGTATCTCCACCCGTACTCT

>HP 14_S SH_trimmed.fasta.Contigl 3

GATGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAG

GCAGGTACCCCTGGCCGAGTGTATGACATGATCAATCGCCGAGTCTTAGA

GCCGAAGGACATCGAGATGTTCGTTCTTGATGAGGCTGATGAGATGTTGT

CACGTGGTTTCAAGGACCAGATCTACGATGTCTTCCGGTTCCTCCAAAGC

GATGTGCAGGTTGTGTTGCTCTCTGCTACCATGCCTGCTGATGTCATGGA TGTAACCACCAAGTTCATGCGAAAACCAATCACCATCTTGGTCAAGAAGG

AGGAGCTGACCCTTGAAGGTATCAAGCAGTTCTATGTGAATGTTGAGAAG

GAAGACTGGAAGTTGGAGACCCTGTGTGATCTGTACCTCGGCCGCGACCA

CGCTGGGCGTACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTT

GTTCCCTTTAGTGAGGGTTAATTGCGCXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXGGCAGACCGTAAAGCCGTGNGGCGTTTCCTAGGCCGCCCTTTTTC

NTTTCTNTCTTCCTTTNTGTCACCCGGAGTTANACAAGGGCCCCGAAGCC

AGGGCCATGA

>HP 14_SSH_trimmed.fasta.Contigl4

GATGGAGCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGC

CGAGGTACCAGTGAAGGAAAGCCTTTCGCCTGAACATAGCAGTGAACTGT

TCGCTGACACACTTGAAGAGCTCCTGGATGGCAGTGGAGTTACCGATGAA

GGTAGAGGCCATCTTGTATACCTCGTGGAGGAATGTCGCACACGGCGGTC

TTTACGTTGTTGGGGATCCATTCAACGAAGGAATGAGGAGTTCTTGTTCT

GGATGTTGTACCTGCCCTGGCGGCCGCTCGAGGGCGTACTTCTGTCGTCT

TGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATT

GCGCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCTTTAGGGCTTCCGGCCCCC

TTGAAGAGCTTTCCAAATTCGACGGCTCAAGTTTCGGAGGTNGGGAAAAC

CGGACGGGGGCTCTTGGTTCACGCGGGTTTCCCCTGGAAGTCCCTGGGGG

GGCTCNCTGTTCGACCTGCGGTACGNACTGCTTCTCCTTNGGNGG

>HP14_SSH_trimmed.fasta.Contigl5

GATGGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCA

GGCAGGTACAGGTCCTTACGGATGTCCACGTCGCACTTCATGATGGAGTT

GTAGGTGGTCTCGTGGATGCCGCAGGATTCCATGCCCAGGAATGAGGGCT

GGAACAGGGCCTCGGGGCAGCGGAACCTCTCGTTGCCGATGGTGATCACC

TGGCCGTCAGGGAAGCTCGTAGGACTTCTCCAGCGAGGAGGAGGAAGCAG

CGGTGGTCATCTCCTGCTCGAAGTCCAGGGCCACGTAGCACAGTTTCTCC

TTGATGTCACGAACGATTTCTCGCTCGGCGGTGGTCGTGAAGGTGTAGCC

ACGCTCCGTCAGGATCTTCATCAGGTAGTCTGTGAGGTCGCGGCCGGCCA

AGTCCAGACGCAGGATGGCGTGGGGCAGGGCGTATCCCTCGTAGATAGGC

ACGGTGTGGGACACGCCGTCGCCGGAGTCGAGCACGATACCGGTGGTACC

TCGGCCGCGACCACGCTGGGCGTACTTCTGTCGTCTTGAGCGTACTGATG

GTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGTCCAACT

GATTCGTTGGCTCCGGGCCTGTCGGTTGGGCGAACGGTATACGNCCCCCA

AGGGGGAAAACGTGTCCCCCGACGGGTCTCNCCCGTCGTCTCC

>HP 14_SSH_trimmed.fasta.Contigl 6

GATGGAGCNCAAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGC

CAGGCAGGTACTTGACACACAAACCCGTGTTTGTTAATAGCCATAATACA

AAGACAGGGTTAACAGACTGGGGCTTCATGTCCGCCGGAAACCTTCAAGA

GAGCAATGGTCAAGGTAATGCTGATTCACTAATCTATTATTCCCTTCCTT

GTCTTGTAGCTACTGATAAACAAGTTTCTTGGGTTGGTGCATTATCTTTT

TTTGGAACACACCAACCCGTATTCAGGGCGCAGTATACTGTATGGTGAGG

GTCCATGAGCAACATAGTGTCGAGAAAAGGGTGGCACTATGAAACCCGTA

GGATGCGATGAAACCAACGTGATCATGGAAATTAGAAGCATTTGCGGTGA

ACGATGCCGGGGCCAGAGTCGTCGTATTCTTCCTTGGTGATCCACATGGA

CTGGAAGGTGGACAGAGAAGCTAAGATAGAGCCGCCGATCCAGACGGAGT

ATTTACGCTCGGGAGGAGTAATGATCTTGATCTTAATAGTAGAAGGAGCC

AAGTTCGTGATCTCCTTCTGCATGCGGTCAGAAATACCAGGGTACCTCGG

CCGCGACCACGCTGGGCGTACTTCTGTCGTCTTGAGCGTACTGATGGTAC

CCAGCTTTTGTTCCCTTTAGTGACGGGTTAATTGCGCXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXGGGAGCCCTA

>HP14_SSH_trimmed.fasta.Contigl7

GCATGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCA

GGCAGGTACATGAGGTAATTTAATTCATGTTCTCTTAAAAATTATAATTA

GAAAAGCAAATAAACCATTTTATTGCACAGCATCTTGGACACACTATTCA

TGGAAGTGAATATACAGCCTCTAAAACATGTGCAATTTCACAATACGTGT

GGAATGTCAGCTCTCTTAACGTATTCTTGAATTTATCGAGACGCGCAAGA

GAAATACAGAAATATCTATTCATTAGTTTTATCTATTCATCATTATTCCT

TTTTTTTTTTITTTTTTTTTTTTTTTTTTTTΓTTGGTTATTTTTTTCAGT

ATTGAATAACTAAGGTGTCATTACTATTCCTATTTTTATTTTTTGGTTAA

TGTTTTTTTCAGTATTGAATAACTAAGGTGTCATTACTATTCCTATTTTT

ATTTTTNGGTCTAGTTTTTTTCAGAATTGAATAACTAAGGTGTTTTCTTG

TTCATTTTCTTGACCAGCGGAGGAACAGCTTTCTCGGCCTTAGGAGACTC

CTTCGCTCCTTCTGAGTGACCTCCGGCGTTGCTTCAAATCCTTCAACTTC

TCGAGCCTAATGAGGTCGTCGACGTCCTTCCTCTCGCCGGCCAGGACGTG

GTTGAAGGCGAGCATGTTGTGGGGAGGTCGCTTGGCCCCCTTCGACCCGC

GGGAGCTCGGGCGGAGGGCGGATGTTGCAGTTGGAGGTGCCCCGGTACTC

GGCGGAACACGCCTGGGCGTANACTTCTGTCGCTTGAGCGTCCTGTGGTA

CCAGCTTTGTNCCTTTNGTGAGGGTTATGCGCGCCTGGGTAACCATGGCA

TACCTGNTCCNGNGTTGAAATTGTTCCGGCCCATTCCCCATTGGGGGNNT

TAGTTTTCGCNGGGGCTCAGAGGAGCCCTAATTGTTGCACNGCCTTCCAG

GGGAACNCTCGCGGTTATA

>HP 14_S SH_trimmed.fasta. Contig 18

ATGAGCTCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGCC

GAGGTACTTAATAAACCCCATATCAAATTTGAAACTATACACATTAATAA

ACTCGAATTATCAATATTAGAAATATGAAAAAAAAGGATATGAAGGAAGG

GATGGAGAAGAGAGAGAAGAGAATGGCAAGGAGGAAGAACAAAAAGAGTT GAAAACGTAGCTAGAGAGAGAAAGAGGTAGGACAGAGCGGAGAGGAGAAG

GAAGAGGAAATGAAGAGAAACGGAGACGGATCGGGAGACACAGTAGACAG

AGGCGGGTGAATCAGCAGGAAAGGCAGCGGAAGGAGCGGCGTGCCTGCTG

AGCTGAGCCACCTGGAGCTCTTCCTTCCTTCTTAAACCAGCTGGAGTCCT

TCCTTTCTGCCTTCCTTCCTTCCTTCTCAAGCCAGGAGACGCGGCATGGC

GACGGGGATCAGCAGGAGAGGCAGCAAGAGGAGCGGCGTGCCTGCTGAAC

TGGTGGGTTTGTCGTTGCACAAGTTTCCTTTGCACACGTAGTTTGTATCG

TATATATAATGGCTCGTGGCAACAATCCTTGATCTCCACCGCGCGCCGTC

AGTTCGCCAAAGTCCGCCCTTACAGTTTCAGCTGCACAAAAACGGCTTTG

AGAGGCTCGCTTCGGCAACCTTTGAGGCCACGGAAGGACTCCGACAACGC

AGTAAGGATTATTGGTTTAGCTCGGTTGTATGAAAGAGAGGATTGTATCT

ATGCACGTGTAGCACTGTGTCGTCCACGGCTTGGCCTGGACGNTGGCGTG

TTGCCCAGGGACAGCACGGCACAANTTGGCCTTGTTGGCTGCCAGTGTTC

CGATGGGAAGCACTGTTTTTGGCCCTTGGCCCCTTTCCTCNCTGTNGCCC

NNGGGTCATTTCTTACGTCTGTGTCCCTTGNCCCTAGNGGATAGGCCGGG

TCACGNATTGCCGATAGTCCCCA

>HP 14_SSH_trimmed.fasta.Contig 19

GAGCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGCCGAG

GTACTTAGGACTCATTTCAAGCTGTTGCGTGTCTATGACCACACTGCATG

TAATGATATGCTATTTTTATATTGTTCnTATATTTCTGTCTATTTCTAA

TACTGTTTTTTTCATATTAAGTATCCAGACCCCCTCCTTTACGTATTTGT

AAAGATAGGGACCTAAGCTTAAGAAACCGTGTCTGCAGCTTGTTATATCA

AAACCCGAGATCATACGTAAGGCAATTGTAAATGTGAATTTTATGCATGT

GTTTTTTTGTGAAATATAAGCAAAACTTTTATTTTGATATGTATTGTTTT

GAGATATTCATTCCATGCAGTTTGATAAATTTGGCAATCCCAGAAAACTG

AATGACAATGATAATCAAGTGATATTAATATACATTGATAATAATTATGA

TGATGAAAAAATTACCTCTATCGATATTCCAGAATTATGTAAAATATTAC

TAAGTCGACTTTTTTCTCAAAGAATATAAGAACTTTGATAAAAATTTGCA

TGGCAATGAAGATACAAAATGAGACAGATATGCATGTATAGTTTGAATCA

TTAATGTTATATCATATTTCATGTTACTTTACATAATATATAAACTGTAA

CCAAATACTATAACGTTCAATAAAGGAAATAGTATTTTTACTCTAATATT

CATATAATGCTAAGGATATGCTTGTAATGTATACTGTTAATACTGACTTG

AAATTAAAGACGCAGATAGAAATAACAGTAAGAAAAAAAACCAAAAAAAA

AAAACACAACNAAAAAAGTACCTGCCTGGCGGCCGCTCGAGGGCGTATCT

CTGCGTGTACCACTGCTTGAGCTCATC

>HP 14_SSH_trimmed.fasta.Contig20

GCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGGTACCATCAGTACG

CTCAAGACGACAGAAGTACGCCCAGCGTGGTCGCGGCCGAGGTACCACAG

GGGGCACCACGAGTCGTATGCGGATGGCATTAACTGGTTCGACTGGCGTG

GACACCATTACTCCCTGAAGACCGTCACCATGAAGATCAAACCAAAGTTC

TAAAAGGTGCTTGAACGGAAGGTTCTAATTCTAAAGATTCTTAAATAGCA

TAATAGGTGTTCATGTAGCAGAAAAATATATTGTAAAATTTGTTCATGTT

GTAAGAAAAAAAATATTTGTTGTTGAACACtAAAGGAAATTGTGATATGC

CCATTTATTTTTTTTTTTAATTTTTTTTTGTTAATGTTTAGCTCTCTTCA

TATTITCTTTTTCTACTTAAAGTAAATTTTTCGTTCACTTTACAAAAAAA

TGTACCTGCCCCTGGCGGCCGCTCGAGGGGCGTACTTCTGTCGTCTTGAG

CCGTACTGATTGTACCCAAGGCTTTTGGTTCCCTTTAAGTTGGAGGGGGT

TATTGNGCCGCCTGGCCGTTATCCATGGGTCCTAGGCNTGTTCCTGTGTT

GAACATTGGTATCCGGTCAACATTCCCACACACTTACGAACCGGGGGCCT TACCGTGNTAAAGGCNGGGGTTGCTTATGNTTNACTTACTCCACTTAATT

GCGTTTGGGCCGTCTACTGNGCCCGGNGGTTTTTCCCCAGATNCGCGGGA

AAACCTCGTTGTCGCTGTCGCCCGCGTTGTGCTTTTATGGGATTTCCGGC

CAAGCGCGGCCGGGGGAAAGGCGGTTTGCGCGTATGGGCGGCTCTTCGGG

TTCTCGGCTACCACTTGAGTCNCGCNNGCCACCGGAGCTTCGGTGTATGG

GGNGACNGCGGGTTTCACGTC

>HP 14_SSH_trimmed.fasta.Contig21

GCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGGTACCATCAGTACG

CTCCAAGACGACAGAAGTACGCCCTCGAGCGGCCGCCAGGGCAGGTACGC

GGGGGCGACGCCGGGTCCTGTTTGTCATCTCCGAAAGAGAAACAAGTCAG

TCCCGATTTCCTTCAGATTTTAAGCATGACAATGGACGACCACAACGAGC

ACCACACAGGATTCCAGTTCGGCAACTTCGCCAGAAATGAGAAGCTAGCA

GCCAAGGGCTTCCCCATGCCCCGGGCCCGCAAGACTGGCACCACCATTGC

AGGGATTGTGTTCAAGGATGGCGTGGTGTTGGGGGCAGATACGAGAGCCA

CAGAAGGTGACACTGTTGCTGACAAGAACTGCAGCAAGATTCATTACCTT

CAACCCAATATGTACCTCGGCCGCGACCACGCTGGGCGTACTTCTGTCGT

CTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAA

TTGCGC

>HP 14_SSH_trimmed.fasta.Contig22

GCCAAGCGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGCCGAGGTA

CGCGGGTAAGCAGTGGTAACAACGCAGAGTACTTGAAATACAACAAAGTG

CTCTGAGCAATGAGGAAAAGTTTGATCAAGAAATATCACAAATACATCAA

ATGAACAATGTATAGTCGAATGCCACGTCATCCAGAAGCATGGCAGACTG

AGAGTGTGTGAACTGAACAATTAATGAATGCTTGATACAAGCGACATATT

CATCATGATGAGTAAATTGATACACAAAGATGTAATTTAGCTTTCGTGAG

TTCACTATTCCTGGAATTCAGTATCAACTAACTGGAATATAGACTGATAT

ATGATTCCAAGAAATATTCACAATAAAATGATCACGAGAATTAAAGGTGA

GATGATCTCTTTCGGTTCGCGCCCAGATAGGAAAAGCTGCATGAAAGGGC

AGAGGGATATTTGCAGTGCTGACAGGAACGATGTTCGGCTGACGTTTGCT

CCCGCTGGCTGACAGGGAGGGAGGAGGGGGTGGGCTGGCAAGAGTCAGTT

GCCAGAGAGAGTCCGAGATCCGCGCAGGGGTCGAGAAGCCACATGTCAAT

CCGGGAGGAGGAACGGTTGGGTACCTGCCCTGGCGGCCGCTCGAGGGCGT

ACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTA

GTGAGGGTTAATTGCGC

>HP 14_SSH_trimmed.fasta.Contig23

GATGAGCCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGC

CGAGGTACTTCCTCCACTTTATGAGGTCACACCTCATCTCTTTACTAACA

GTGAAGTCATTGAAGCAGCATATCGTGCCAAGCAGACACAGACCCCTGGT

AAATTTAAGTCTTCCTTTACTGGAACAAAGAAGAACCCTGAACAGAGAGT

AGCCTATTTCGGAGAGGACATTGGAATGAACACTCACCACGTTACCTGGC

ATATGGAATTCCCATTCTGGTGGCAAGACAGTTACAGTCATCACCTGGAC

CGCAAAGGAGAGAACTTCTTCTGGGTACCTGCCCTGGCGGCCGCTCGAGG

GCGTACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCC

TTTAGTGAGGGTTAATTGCGC

>HP 14_S SH_trimmed.fasta.Contig24

ATGAGCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGCCG

AGGTACATGCAGGATCTAGGAGGAGAAGGGGAAGTGCTGCAACTAGCTTG

GAGGATTGTAAATGATTCCCTTCGCACGGACGTCTGTCTTCTATTTCCCC

CTTATGAAATTGCGTTATCTTGCATCCATATGGCATGTGTCGTCCATCAG AAGGATTGCAAGCAGTGGTTCGCCGAACTGAACACTGATCTGGACAGGCT

CATGGAAATCACAAGGTACCTGCCCTGGCGGCCGCTCGAGGGCGTACTTC

TGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTAGTGAG

GGTTAATTGCGC

>HP14_SSH_trimmed.fasta.Contig25

GCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGGTACCATCAGTACG

CTCAAGACGACAGAAGTACGCCCAGCGTGGTCGCGGCCGAGGTACAAATT

ATTTATGGTCTGGAGTTGAAATTAATTCCCCGGTTAATTTTGATATGGAG

CTTACAGTTGTTTCCGCAATAATTGCATTCTCCATAAAACGTTTGAAAAT

GTCGAAGAGCCCCGAGGGATCATCGTGTGCCTAAACTCTTTTAATCCTGG

CAAAATCCTTGTTACTTGTCGAAGGAATAGCGCCTATTACTCGTGTTAGA

ATGTTATTTCAACACCCCTTTTATATGGCCAGCCAAACTAGGCTTCATCG

GCTAACACTCTGCTCTCGGCTTTTGTGGCGAAGCGGAGCGTTTTGGGCCT

GCGGTGAGCGGCTCTTCCGAACACCTCGCTTCCGTTTCGAAGGCTTGACG

ATTGGTCACGGTAAATCGCGTTTTTCTGATGTTCCTAAGGTGTGAAAGGA

AGCACGAGACGCTTAGTATTGCAACGATGAAGTTTAGTCGGGCTGTCCAC

ACTGTGTACCTGCCCTGGCGGCCGCTCGAGGGCGTACTTCTGTCGTCTTG

AGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAAATTG

CGC

>HP14_SSH_trimmed.fasta.Contig26

GTGGAGCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGCC

GAGGTACGCCTGTCGATCTTGGTCAGCAGTTCGGCGAACTTGCAGGCAAT

GTGAGCAGTGTGGCAATCAAGCACAGGTGAGTAGCCAGCCTGGATCTGGC

CAGGGTGGTTGAGGACGATCACCTGGGCGGTGAAGTCAGCAGCTTCCTTG

GCTGGGTCGTTCTTCGAGTCGGAAGCGACGAAGCCACGCTTCAGATCCTT

CACCGACACGTTCTTCACATTGAAGCCAACGTTGTCACCAGGGACAGCCT

CAGTAAGAGCTTCGTGGGTGCATCTCCACAGACTTTACCTCAGTGGTGGG

GCCAGTGGGGGCAAAGTTGACAACCATACCTGGCTTCAGGATACCAGTCT

CCACACGGCCCACGGGCACTGTTCCAATACCTCCAATCTTGTACCTGCCC

TGGCGGCCGCTCGAGGGCGTACTTCTGTCGTCTTGAGCGTACTGATGGTA

CCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGC

>HP 14_SSH_trimmed.fasta.Contig27

GATGGAGCCAAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGG

CCGAGGTACTGAAGGAGCGACTGCCACTGCCAGCTGACGACAGCGTGAGT

TTTATGTCAGATCCAAAGTGCATCATTTGGACGCCTGTCTGCCGCTCTGA

CATTGCCTGGAACTTCGAGAAATTCCTCATTGGCAAGGATGGACAGCCGT

TTAAGAGGTTCAGCAAGAAGTATGAAAGCCATATTTGCTTAAAGATGAAA

TCGCAAACTTGCTGAAAGCTTAAAGAAAGAAGATATCAGAAGACAAGGTG

ATCCAAAATTGTAGGAAAAAAAGAAAAATAAACATGAAATAACCAACAAT

TTGCACCAAAACAACAGAAAACGGAGAGCGGAGAAACAGAAAACGGAAAA

CCATTCCAACCGCCAACCAGCTGATCCCTTTTCCGTGCAAAAAGGGCTTT

AGGGACCTCAATAATGAAGGGAATCCTTCAAACACTCTTAAGTGCTAGGG

TTCTTGGATGATAAATTTCGAGGTCCTGGGGTTCTAGGATTTATTCCTTT

TTTTTGCATGGGCGTAGGATCAGTTAAGAGTTCCTTTAGAATACTTTTGA

AGAGATAAAGTCAAAAAATATTTTCTTTTCTTTTATAAATGTCTTGTGTG

TTAGTGGCATCGCGTATTATATATTTGGAGCAAGTCGATGTGATTTGTTT

ATATTAAAAACGGACATTATTTTACACACAAACNAAAAGTACTGCCCTGG

CGGCCGTCGAGGGCCGTAACTCTGGCGCTGACGTCTGTTGGTCCCCAGTT

TGGTCCCTTAGTGACGGTTAATTGGCGCTGGGGTAACCTGGGCNTAAGCC GGTCCTGGGNTGAGTGGTTCCGGTCCAATTCCGTTCTTNNCTCGNNCGCT

GTNTNTCNTTGCGCTCGGGTGCGGTCTGGGGGCCACCTAATGTGGCCAGG

CGGCTACAGGAACACGTGCGGNTTATGCACCGGGAGGTATTGGCCCCTTA

CAACACGGCGGGGTGGGCATTGACGCTGGTGCTCGACCGCCTCCNCCTCG

CTATT

>HP 14_SSH_trimmed.fasta.Contig28

GATGGAGCCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGG

CCGAGGTACTTCTCTACAAGAAACGCCTAGACAGTTGAGGAACGTTCAAC

CTTTTGCTGCACTTGACTTACCCTGATGAGACATGTCGCTGAATACAATA

AGTATATATCGATGCATATTTGTGAAGAAATGGAGACTGGAGATGGCATG

CTAAGTGGTCCGTCTATACTGTCGAAGTATGTCATCACAGGTCACGTTAT

TTTTTAAAAATCTTATACCGAATATTATCTATTCAACCAACAACTAAATC

TTAATAAAATCTGGACAGATCGTGATTTCTTTTGGCGAGAGTTGCGTCTC

ACACTTTGTTTGCTTTGAGAAAAGTCCTCATGAATTCGGTCAAGCGTCTG

ACGTCATCGATGGCGATAGCATTGTAAAGCGAAGCGCGGATTCCACCAAC

CGACCTGTGTCCCTTGAGAGAAAGAAGTCCTTGGGCAGCAGCCTGTGAGA

TGAAGTTCGCCTCGAGATCGTCTTTCCCGCCTTCGCCCACCCGGAAAACG

ACGTTCATCCGGGACCTGGCAGCCACATCCACAGGATTGCTATAAAAGCC

GGCGCTCTCGTCGATAACGGCATACAGCATCTTGCTCTTCTCAATCGAGC

GTCTTTGCATCTCCTCCAGGCCGCCTTCCCGCTTCACCCACGCCAGGGAC

CTCGCCCACCACGTGGATCGCGAAGGTCGGCGGCGTGTTGTACCTGCCCT

GGCGGGCCGCTCGGAGGGCGTACTTCTGTCCGTCTTGAGCGTACTTGATG

GTACCCCCAAGCTTTTTGTTCCCTTTTAATGCACGGGGTTTAACTTTGCA

GCGCNTTGGCGTAAATCACTTGGTCAATTAGCTGTTTCCCTGTGGTGAAA

CTTGTAATCCGTCACCAATTTCCAACAACAAAGCATACGAAGGCCGGGAC

ACTTAAAGGTTGTNAAAGC

>HP 14_S SH_trimmed.fasta.Contig29

GATGGAGCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGC

CGAGGTACCCCTGGCCGAGTGTATGACATGATCAATCGCCGAGTCCTAGA

GCCGAAGGACATCAAGATGCTCGTTCTTGATGAGGCTGATGAGATGTTGT

CACGTGGTTTCAAGGACCAGATCTACGATGTCTTCCGGTTCCTCCAAAGC

GATGTGCAGGTTGTGTTGCTCTCTGCTACCATGCCTGCTGATGTCATGGA

TGTAACCACCAAGTTCATGCGAAAACCAATCACCATCTTGGTCAAGAAGG

AGGAGCTGGCCCTTGAAGGTATCAAGCAGTTCTATGTGAATGTTGAGAAG

GAAGACTGGAAGTTGGAGACCCTGTGTGATCTGTACCTGCCCTGGCGGCC

GCTCGAGGGCGTACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTT

TTGTTCCCTTTAGTGAGGGTTAATTGCGC

>HP 14_S SH_trimmed.fasta. Contig30

GATGGAGCTCAAGCAGTGGTACACGCAGTTAGTACGCCCAGCGTGGTCGC

GGCCGAGGTACATGGTGGTACCACCAGACATGACAATGTTGGCGAACAGG

TCCTTCCTGATGTCAATGTCGCACCTCATGATGGAGCTGTGGACGGTTTC

CTGAATACCAGCAGATTCCATACCAAGGAAGGAAGGCTGGAACAGAGCCT

CAGCGCAGCGGAAACGCTCGTTACCAATGGTGATGACCTGACCGTCGGGA

AGCTCGTAGGACTTGTCCAAGGAAGAGGAAGCAGCAGCGACGTTCATCTC

ACTCTCAAAGTCAAGGGCGATGTAGCAAAGCTTCTCCTTGATGTCACGAA

CGATTTCACGTTCAGCGGTGGTGGTGAAGGAGTAGCCACGCTCAGTCATG

ATCTTCATCAGGTAGTGGGTAAGGTCACGACCAGCCAAGTCGAGACGGAG

GATAGCATGAGGAAGAGCGAAACCTTCATAGACGGGGACGAAGTGAGTCA

CACCGTCACCAGAGTCGCAAACCTCACCGGTAGTACCTGCCCTGGCGGCC GCTCGAGGGCGTACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTT TTGTTCCCTTTAGTGAGGGTTAATTGCGC >HP14_SSH_trimmed.fasta.Contig31

GTGAGCCCAAGCAGTGGTACACGCAGAGTACGCCCGTGGTCGCGGCCGAG GTACTTTTTTITTTΓΓTTITTTTTΓITGTTTTTTTTTTTAAAAGCGACAA

TTTATTTGCACTACACCTTACTTAATAGAGCTTCATTTCCTGTCGCTGCT

CAGAGCTAACCTTGTGGTTCTTAGCAAGGTAGTCAGTGAACTCCTGGTAT

GGAGACTTAGTGAAGACGGCCTCCTTCCAGAGGTCTGGTGTAAGGTAAGC

ATATGTTTTGGCAATGGCAGCATATGCAGCCTTGGCAAAGTTACCGAGAG

TGGCAGTCTGTCCACGGGCACTGGTGTAGCAATCCTCAATACCCGCCATG

TTCAACAGCTTCTTGGGTACACAGATCGTATTTCTTTTGTAAATGATCGT

AATTTCCGATTTCTCTTGTGTCTCAGCTGTTCGTTTTTTTTCTTCTCTGT

CCTTATCTTCTCTTGGATTTACTGTCTTGGTCTGATTTTCATCTCTGATA

ACTTGAGGGAGTACCTGCCCTGGCGGCCGCTCGAGGGCGTACTTCTGTCG

TCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTA

ATTGCGC

>HP 14_SSH_trimmed.fasta.Contig32

GATGGAGCCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCC

AGGCAGGTACTCTCTCTGACTACAACATTCAGGAAGAATCTACTCTCCAC

TTGGTCCTCAGACTCAGGGGTGGCCAGTGAATATTCCATTGTGATCAGTT

TCCAAAGATTTCCTGTCAACTACGCTTATTGCAAAAAAATGTAATTGCAC

ATTATTCCTTTCCAGTGTATGTGGAAATTATATGAACATGATTTTATTTC

AAATGCACATCAATATTATTTTTTACTTTTTATAATGGGCTAGCAGAACC

AAATAAAAACTTTATTTGCTAAAAAAAAAAAAAAAAAAAAAAAAAGTACT

TTAAAATACAACATAGTGCTCAGAGCAACGAGTTAAAACTGTAGCAAGAA

ACATCACCAAAACATCATATTTACAAAAAGAGTAACATTTTCTAGTCAAT

TGCTAGTCTGCCCAGACACACCGCTGGCCGAGAGGGAAGGAAGAGAGCAG

CTCTGTTAGAATGGATGCTTGATTGCAATGTGTAAACTGTAATGTGTAAA

CGATGGAGCAAAATACAGTGTAACATAAGTGAGATCGTCATTCCGGGAGG

TAAACGTCAACAAACTGCCAATAGTTTAGAAAAGTCATCGATTCCTTCGG

GCTAGTCGCGTCGAGTTAGTAGTTGTATCAGAAGTAAAGGAACGCTTGCA

GAGCTGACTGAGATGTTCGGCTGACGTTTGCTCCTGGAGGCTGTGGGGAA

AGGGGGGCCTGGCAGGGGTTAGTTGCCAGGGACAGTCCGAGATCCGTTCA

GGGGTCGAGTTGCCATATGTCAGTCCGGGGACGGTAGGTTGGGTACCTGC

CCTGGCGGCCGCTCGAGGGCGTACTTCTGTCGTCTTGAGCCGTACTGATG

GTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTTGCGC

>HP14_SSH_trimmed.fasta.Contig33

TGCGCGCTAATTTAACCCTCACTAAAGGGAACAAAAGCTGGGTACCATCA

GTACGCTCAAGACGACAGAAGTACGCCCTCGAGCGGCCGCCAGGGCAGGT

ACTTGCAAAGGACCAGGGTAGAAGGGCTAGTCAGCAAGATCAATGTAGTG

CTGCTTCAAGAGATCTTCAGTTGCCTGAATGTACAGCAATCATTATGAAC

ATGCAAGGGATTTCAAGACAGAGAAGACATCACACCACGCCAGAAAAATC

ATACTTACTAATCCTTCTTTTCCTCTTTCTCATCTCCCTCTTTCTTCTCG

TCTTTCTTTTTATCTCCATCTTTCTTCTCCTCTCCGTCTTTCTTCTCCTC

CTCATCTTTCTTATCTCCATCCTTCCCGTCCCCATCTTTGTCCGTGTCCC

CGCGTACTTTATTCTATAACTCTTTTTAATTATTTTTTCGATTCACTCTA

CTAAAACAATAATATTTCAAAATATTTTTATTACTTTTAAATTTATTTAT

TGCAAATTATTTACATTAGCAAGGTTTAATTTCCCGCGTACCTCGGCCGC

GACCACGCTGGGCGTACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAG CTTTTGTTCCCTTTAGTGAGGGTTAATTGCGC

>HP14_SSH_trimmed.fasta.Contig34

GATGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCGCCAGG

CAGGTACGCGGGGAGTTGCCACTGCAGCAGCACAACGAACCGGTGCACAA

GAGACTCTCTCCTTTCTTTCAGTATGAAGGTGACACTGGTCCTGGTCCTG

GTGGCCGTCGTCGGCCTCTCCTCCGCCTACTCCCTCGGCGACGTCTACGA

GGAGCCCCATCGCGTCGAGAGGGACATGAGCTCAGGGACGAGCGGAAGCA

CGTCCGTGGAGAGGATAGAGAAGAGAGACATCAGCACAGGGAGCACCAGC

GACAGCGCGTCTTTTGAGAGAATAGAGAAAAGGGACATCGCTGCCACTCA

CGACTTGGATCTGAGGCGCCTCAGGAGACAAGCCTCCAGCTCCGAGAGTG

AGAGCTCTGAGGAATCTTCTGAGGAATGGGCAACTGTCGCTCCTTGAAGG

ACTCATGTGACGGCGCTGACCTTTGGCGAACGACCTGACCTCCATTTGGC

CCTGATGATTTGAATTAGGATATGTCGAATAGTTTTCAAAATTCTTTAAA

CTGTCTGGTAAGACACGTCATTGGATAGAATTTTCAGCTTTCATGTAGAC

AATAATCTGCATTTTGTGTAACCAGAATTAATAAAGAGATTGGTAAAAAA

AAAAAAAAAAGTACCTCGGCCGCGACCACGCTGGGCGTACTTCTGTCGTC

TTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAAA

TTGCG

>HP14_SSH_trimmed.fasta.Contig35

GCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGGTACCATCAGTACG

CTCAAGACGACAGAAGTACGCCCTCGAGCGGCCGCCAGGGCAGGTACAGA

AACTTATGAGAGACCTTAAGGATCACAGACTTCTTGAACAGAAGCACTGG

TTCTCTCTCTTCAACCCAAGACAACGTCAGGAAGCACTCATGCTTTTCGA

TGTTCTCATTCACTGCAAGGATTGGGATTCATTTGTCAGCAACGCTGCCT

ACTTCCGTCAGCGTATGAATGAAGGAGAGTTTGTCTATGCCCTGTATGTT

GCAGTCATCCACTCACCTCTGGCTGAGCACGTTGTACriTTTTTTTTATT

TTTTTTGACTACTCATATTTTATTATAATTTTCAGATCCATTTATATAGG

AAAACAGCTTCCTCTGTGAGGCACTTCGCTTTTAGTGTCAATACTTCCTG

TGGCATCGGTTCATGGCCCTTCCTCTGTGGGTACCTCGGCCGCGACCACG

CTGGGCGTACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTGT

TCCCTTTAGTGAGGGTTAATTGCGC

>HP14_SSH_trimmed.fasta.Contig36

GAGCCAAGCAGTGGTACACGCAGAGTACGCCCGCGTGGTCGCGGCCGAGG

TACACTTCAGAATGCAATGGGGGCTGTGCTGTTAACTGGGTTTGAGATGA

TGCAGATCATAGCATTGGGGCAATTTTCAGCACAAGCCTTGGCCAAGTTT

GCAACAATAGAGGCATTGGTGTTGAAAAGATCGTCTCTGGTCATGCCAGG

TTTGCGGGGAACTCCAGCAGGGATGACGACAACTTCACATCCCTTCAGGG

AATCTGCAAGTTGGTCGGGTCCAACAAAGCCGGTGACCTTGGCTGGGGAG

TTGATGTGGGAGAGGTCAGCAGCTACTCCTGGCGTGTGCACAATATCGTA

AAGGGAGAGTTCGGTGACCAAGGGAGAGTTCTTCAGGAGTAGGGAGAGTG

GCTGACCAATGCCTCCACTGGCACCCATGACAGCAACCTTCCTGTTGGCG

GCATTGGTGGTGGAGAAGTTCTTGACCAGCTGACCAAGGACATTGGCAGT

CTGGGGCTTGGCAAGTCTGGAAAACATGATTATTGGTGTTGAAGTGCCCC

GCGTACTCGGTATTCTCATGAAGGTTCGGCACTGCTGAGTCGGCATCACC

ATCGGTCACCGCCACCACAAGGTCGAATTCCAGGCCTCTATCGTTGCCCT

TGGGGAGAAGGAACCTGTTTGGCAGGCCTGTTGCACTTTCGAATTCGGAA

AGGCCAGTGCCGCCTGCCTCGACTTTTGCAAACAGGTCCTTTATGCTTGG

GACGTCCGGTACCTGCCCTGGCGGCCGCTCGAGGGCGTACTTCTGTCGTC

TTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAAT TGCGC

>HP14JSSH_Mmmed.fasta.Contig37

GCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGGTACCATCAGTACG

CTCAAGACGACAGAAGTACGCCCAGCGTGGTCGCGGCCGAGGTACAACGA

AGCCTCGGGGGTGTGCGAGGATCCGTGCCAGTGGAACACGGGGAAATTCT

CCTGCATCGCGGAAGGCCGTTACCCCGATCCCCTCAGCTGCTCTCGCTAC

TTCGAGTGTGTCGTGGATGAGGCAGAGGAGACAGGCTACCGTCAGGAGTT

GCACGAATGCCCTGAAGGATACGTGTGGGACCCCAGCGCCAGGGGAGACT

TCGGCCACTGTGTCCTGGAGGGTGCGAGCAAAACACCCTGTTCGCCAGTC

ACCATCAACAAGTGCACCATCCCGGACAACTGGTGTGACAACACCATTCA

ATAAGTCCTTCAAGCACTGACATTCCCTCTGCTTTCTCCTTCCCCGGTCT

TAGTAATAAAACAGGGAATGGAAGAAACATGGTATAGGATGTCTGTTATC

ATGGATGTACAACAGGATTCAGAAGGACACGAGCACATTCGCCTGTGTAT

TCTGATTCCATCCACGCAGATTCAGAGACTGCTCTATGCGAGTCTGTTCT

CTGTGCGATTCCGGGAATTTATGATCTGTTTGTTATTTATTGATTGTTTT

CAGGTTGTTTGTTATCTGTTATTCTTAATTGTACCTCGGCCGCGACCACG

CTGGGCGTACTTCTGTCGTCTTGAGCGTACTGATGGTACCAGCTTTTTGT

TCCCTTTAGGTGAGGGTTAATTTGCGC

>HP 14_S SH_trimmed.fasta.Contig38

CAGAAGTACGCCCCAGCCTGTGTTCGCCGCCGGAGGTTACTGTGCACTCC

GTTAAGTGACGTCAAACCCAAGAATGTTGCCCTCCAGTAGACTTAAGGCC

GGAAAGTCCAGATCTTCATGGCATCAGTGGCATCGAACTCGTAGTGCTCG

CACAGTTAGGACCTTCCTGGCCTGGGTTCATCCACCGGGGAGTTGCCTTA

CCAGCCTCAANGTCCCCCGCCGTACAGAAACTTGTTAGAGACCTTAAGGA

TCACAGACTTCTCGAACAGAAGCACTGGTTTTCTCTCTTTAACCCAAGGC

AGCGTCATGAAGCACTCATGCTTTTCGATGTTCTCATTCACTGC AATGAC

TGGAATACATTTGTCAGCAACGCTGCCTACTTCCGTCAGCATATGAATGA

AGGAGAGTTTGTCTATGCCTTGTATGTTGCGGTCATCCACTCACCTCTGG

CTGAGCACGTTGTGCTCCCTCCACTCTATGAGGTCACACCTCATCTCTTC

ACTAACAGCGAAGTTATTGAATCAGCTTATCGTGCCAAGCAGACACAAAA

ACCTGGTAAATTTAAGTCTAGTTTCACTGGAACTAAAAAGAATCCTGAAC

AGAGAGTAGCCTATTTCGGAGAGGACATTGGAATGAACACTCACCACGTT

ACCTGGCATATGGAATTCCCATTCTGGTGGCAAGACAGTTACAGTCATCA

CCTGGACCGCAAAGGAGAGAACTTCTTCTGGGTACCTGCCCTGGCGGCCG

CTCGAGGGCGTACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTT

TGTTCCCTTTAGTGAGGGTTAAATTGCGCG

>HP 14_S SH_trimmed.fasta.Contig39

GGGATGGAGCTCAAGCAGTGGTACACGCAGTAGATACGCCCTCGAGCGGC

CGCCAGGCAGGTACTAGGCCGTCAAGGTGATCCTCATGGAAAGTTTGATT

TACCACCTGGTGTGCTGGAACACTTCGAAACTGCCACCCGTGATCCCAGC

TTCTTCCGGCTTCACAAATATATGGATAACATTTTCAAAGAACACAAGGA

CAGTCTACCCCCATACACCAAGGCCGATTTGGAATTCTCTGGCGTGTCTA

TCTCAGAGCTAGCCGTTGTAGGTGAACTGGAGACCTACTTTGAAGATTTC

GAATACAGTCTTATCAACGCAGTTGATGATGCTGAAGGAATCCCAGATGT

GGAAATCAGCACATATGTGCCTCGTCTTAACCACAAAGAATTCACTTTTA

AGATTGATGTAGAGAATGGAGGTGCTGAGAGATTGGGCACAGTTCGTATC

TTTGCCTGGCCTCATAAAGACAACAACGGAATCGAGTTTACATTTGACGA

AGGTCGCTGGAATGCCATCGAATTGGATAAGTTCTGGGTAAATTTGAAGG

GTGGAAAAACTACAATTGAGCGCAAGTCCACGGAATCTTCGGTAACTGTA CCTGCCCTGGCGGCCGCTCGAGGGCGTACTTCTGTCGTCTTGAGCGTACT

GATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTTG

GGGCGGTATCCATGGTTCCATAAGCTGTTCCCTTGGTGGTGACACTTGTT

TTCCGGCTTCACCAATTTCCAGCACAATTT

>HP 14_SSH_trimmed.fasta.Contig40

GCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGGTACCATCAGTACG

CTCAAGACGACAGAAGTACGCCCAGCGTGGTCGCGGCCGAGGTACAAATG

TAAAGAGAGGAACAACGACACTGCTTTTCTACCGCTGGGCAGCCATCACA

ACGCAACGACCTTCCTCCCCTTCGTCAGCGGCGATGCCCTGATGACCTCC

TTGAAGGCCACCACTCGCCGCCGCCCGAGCACCAAGGGATCCTCCGGGAG

TCAGAAGTACCTGCCCTGGCGGCCGCTCGAGGGCGTACTTCTGTCGTCTT

GAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTG

CGC

>HP 14_SSH_trimmed.fasta.Contig41

CAAGCAGTGGTACAACGCAGAGTACGCCCGTGGTCGCGGCCGAGGTACTT

ACAACCATCAAATGCACTTTTCTTGTAACGGTCATCTACCTTCCAAAATC

CCACGGCGATGCTTGTCTTTTCACACTTAACTTTCTTGAGGATTTCTGGG

ATTTTGTTGACAATCGCGTGCGTGC AAGTGTAGCCAGAAAAAATTCTCTG

AGGATTATAATCTGGAGCGTTGTAGGTGAAGAGAAAGAGTTCCTTGGGGC

ATTCGTCCCTTTCACTCTTGGGAATCTCCTTCTTGTCCTTTTTTTTCGGG

TCTCTACCTCCGGCATGCTCCTTCTTCCATTTCTCGAGCATGGGTTCTAG

TGCCGTGTGGATCAACTGCCATTCAGAGTGCCTTCTTGTCTCTTCACGTC

CCTTTAAATCCATTTCTTTGAACGCGGGGGCAAAGTTGCAATGGGCGTGA

ACTTCCCCGGCTTCGTCAAAGTTTATTCTATGGCAATCTGTGCCATTGAT

GTCCTCTCCTGGCAGATACAGCACAGCAAACTGACGTTTCTCGGAGAGCA

TGTATTTCCCCTCCTTTTGCATTTTATTCAGAGTGTCGATGGCAGTCTCT

GTTGCAACTATGTGGCAGAAGAGGAGCGCCAGCAGGGAACCCAGGAACCA

GAAAAAACGGGAAGCCATCGTCACCCGCTTCTCAAGCAGTCTTAATCTGC

ACTGATGGCCTTGAAAGTCTGGTAGAAACCTTGAAAACCTGACCTAGTCA

GGGTGTGCAGCTGTACCTCGGCCGCGACCACGCTGGGCGTACTTCTGTCG

GTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTT

AATTGCGC

>HP 14_SSH_trimmed.fasta.Contig42

GCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGGTACCATCAGTACG

CTCAAGACGACAGAAGTACGCCCTCGAGCGGCCGCCAGGGCAGGTACATC

ATCAGCTGACCGTTCGCTTTGATGCCGAACGTCTCTCCAATTACTTGGAT

CCCGTCAGTGAACTTCACTGGGGTAAACCCATCGTTCAAGGTTTTGCTCC

ACACACCACTTACAAGTATGGCGGTCAGTTCCCCTCTCGTCCAGATAATG

TAGACTTCGAGGATGTGGACGGTGTTGCCCGAATTCGAGATCTGCTCATT

GTTGAGAGCCGAATTCGTGATGCCATTGCCCATGGATACATTGTGGACAG

GGCTGGCAATCGCATTGACATCATGAATGAGCGTGGTATTAATGTCCTCG

GAGATATCATTGAATCATCAATGTACCTCGGCCGCGACCACGCTGGGCGT

ACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTA

GTGAGGGTTAATTGCGC

>HP 14_SSH_trimmed.fasta.Contig43

TAGGAACCAAAAGCGGTACCATCCNTTACGTTTCAGCGACCGAAGTACGC

CCAGCGTGGTCGCGGCCCGAAGTAACCCNAGAAGTAGGAGAATAGGAATG

AGAAGTCCAATGGGGTGTGACGTCCGTGTTGGTAGATGAGTGATACCGAA

AAAAGAAAGGATAAATGTTTATAGGATATCATAACAGATATATGATAATA TATGGTGTACTTTTCTGGCATTCAATACATTACATACAATTTACGTAAAC

AAAACTGATTTAGATTGTGACCCACATAATAATGGCAATGAAAATATAAC

AGAGGGCAAAACTCCCATTGGCTTCGGTAATGAAAAACTACTTTCTCAAG

GTTGCTCCCGCGTACCTTGTGTATTAGGGAAAATCAAAATAATCTTAATA

AGATAAGTAATCCCGAAAGAGAAAGAGCTAATATAAATAGATAGTTTTCG

TATTAATGAAATTATAAAATTTATATTAGTAGTGAAATGCTAGTCGATTT

TTCATATCTGGTTGATAGAGAATTAAATTAAATTTAGTTGCTTTGAGGTC

GAAGAAAGCTTTAAAGTAATAAAGAGTAGGAGGGAAGAGCTTCTTATTCT

AATAATAAGTTTACTATAACGAAATGGATTTAAGTATTATTGAATTAGGC

TTAAAAACAGCCATATTAATAAAGCGTTCTAGTTAATGTAAATTCATTAA

TTAATATTTATATAGTGAAGGTTTAGTAATAATTATCTGTCTCTTTTAAT

AAATATATCATTAAAAGTAATAATGATTTTATTAGTAGAAATAAGTTGTG

TAGTAACAATTTAAAATAAGGAAAGAATTTTTAGTTGATATAAGATATAA

TTAAGGAACTCGGCAAATAATGCTTTTGCCCTGTTTATCAAAAACATGTC

TATATGATTGGTATGTAAAGTCTGGCCTGCCCACTGATTTATTTTAAAGG

GCCGCGGTATACTGACCGTGCGAAGGTAGCATAATCATTAGTCTCTTAAT

TGGAGGCTTGTATGAATGGTTGGACAAAAAGCAAACTGTCTCAATTATAT

TTATTGAATTTAACTTTTAAGTGAAAAGGCTTAAATAATTTAAGGGGACG

ATAAGACCCTATAAAGCTTTACAATAAGTTATTTATATTATAAATTGTTA

GTATAACTTGATTTTAGATAATATTTGTTGCGTTGGGGCGACGGGAATAT

AATGAGTAACTGTTCTTAAGTTATTTAATAACAGTAATTTCTGGAAAATT

AATGATCCTCTTTTAGAGATCATAAGATTAAGTTACTTTAGGGATAACAG

CGTAATCTTCCTTGAGAGTCCATATCGACAGGAAGGGTTGCGACCTCGAT

GTTGAATTAAGGTTTCCTTATAATGCAGCAGTTATAAAGGAGGGTCTGTT

CGACCTTTAAATCCTTACATGATTTGAGTTCAGACCGGCGTGAGCCAGGT

CGGTTTCTATCTCTTAATTTTATTAAAGTTATCTTAGTACCTCGGCCGCG

ACCACGCTGGGCGTACTTCTGTCGTCTTGAGCGTACTGATGGGTAGCCCA

GCTTTTGTTCCCTTTCGTGAGGGTTAATTGCGC

>E:\AnalyzedData/50513_2_2_Ql_Kubota_Hpl4_02Run01_Cp312_MDl/Ql_Kubota_Hpl4

_02_02B01.F.esd 486 MegaBACE.

GATGGAGCCCAAGCAGTGGTACAACGCANTAGTACGCCCAGCGTGGTCGC

GGCCGAGGTACAACAATCTTAAGAGATTTTAACAGTCACTTGTCTTGTTT

GATTATTTCTCATTCTGTATAAATATCTATTTATATGCAGATATGGAGTG

CACTCCATAATCATCTGGATATAATACTGCATTTGAAAAAATCTATCTTT

GTTCAGTATCAGCATAAATGGCAAAATTAATGTAATTACATGTATTACAG

CATAACATCTAGTTACTATTATCTGGTATTCCACAGCAGTTGCCATCTTC

TCATTGCATAAACCAAATCCATTCTAGTGTTATATTCTAGAAAAAAAACC

CCACATATGTTCAAAAATACGAAAATCTTGTTAAGTTGCAATTAAAAAAG

AATAAAAATCCATAGCATCTGTTATCTCACAGCCACAATTTACGAGTACA

GACACTTCACTGAACATTCATTGTCAGACAACTTTC

>E:\AnalyzedData/50513_2_2_Ql_Kubota_Hpl4_02Run01_Cp312_MDl/Ql_Kubota_Hpl4

_02_10B02.F.esd 944 MegaBACE

TGGAGCTCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGCC

GAGGTACATTGATGTCAAATTATATATAAATCTGGAATACTTCTGTTCCT

CCATAATTCAACCCTAGACTCTAAATTGCTGACCTTTTTACAACTTCGGA

AAGATGGAAAAATTCTTCTTACAGAATATACATCCTAATAAACTGGTATG

TTTCTTAAAATAATAAAAATTCTTGGCAATATTCTTGAAGACATAATGAC

AGTGACCACAACTTCAATGGAAAAATTGCAGCTAAGAACTTCCTTCATCA

CAAAACTGATTGTTCTTGATTTCATAAATGCTCAACTGGTGCTGATGCCC TGACGACCTTAAAAATTATCCCTTACTCTTCGTAAAATATGTATATATAT

AAGATCAAGATAAATCAACACAAAATGTAAATTATCACATAATCCACTAT

AATAAAATATAGATCATCGAAGATAGAGAGAGAGAGATAGAGAAAGAAAG

AAAATGGATAAAAAACAACTGATAAGGCAAAATGGATATGTCACTCTCTC

AGATGGGCCTTGTAATAATTCATCAAAGCTCTCTATGCTGCAATCAATAG

CCCACTCTTTTAAGTTACATGTAAGTTTCTGGGTAAAAATAAAGAAAAAA

TATGAAACAATGAACGAAGAACAGAAAAAATTTGATCCTTGGGATGAAGC

CAAGCATTCTAAAAACCAGGNACCATGGTCCATTAAAAGGCAAAATAAAA

ANAAATACNTGGTAAACTTGGGTTAGAAGGGTAAAGGAGAGAAGAAGGAA

GAGAGAGAGCAGCAGAAGGAAGAGAAGAGGAGAAGAGAAAAGCCGGGGTA

CACTTAGGCTGGGGGCCGGTCGAAGGGGTAACACTGACGCGGAGGTACGN

ATGCACCCATTTGGCTCTCTGCGACACGCNANANTCGCCCCTCG

>E:\AnalyzedData/50513_2__2_Ql_Kubota_Hpl4_02Run01_Cp312_MDl/Ql_Kubota_Hpl4

_02_23G03.F.esd 955 MegaBACE

GGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAGGCAG

GTACGCGGGAGCCATATGGCACTGCCCTGCCACCTGAACTAACTGCCTCT

GACGCCACAGGTTTCCAAGCTTTCTTGGGCCAGTCTCTTGGAGGAACAGG

AGGTATTAAAGATGATTCGGGTCAAGGAACCACAACTGCATTTACTGAAG

CCATTTGTGCCCATCTTGGCACAAGGGCTCTTCTTACACCTGCTGATGCC

AAGGAAGCAAATTTGGCTTTACAGTCAACAGAGGCTACTAAAGTCCCAAA

ACCTGCAAAACAGATGCCTGAGAATGCAGCAGCTATGCATCCCGTCATGG

TTCTGCATCAGATGAAGCCAGGCCTGCAGTATGCTGTAACCCAGGCTACA

AAGGAGAACAAACCATATTTCACGGTTTCTGCTGACATTGATGGGAAACC

ATTTACAGGAGTAGGACACAACGTGAAGAAAGCGAAGTTTATTCTAGCCA

AAGAAGCCATTCAAGAATTGTATGGCATTGATTCAACCTTTGAGGCTCCG

GCΓΓAACATGAATACAACTTTTGTCTTAAGGAATAΓΓTTTTTTAATCTAA

TTGCTATTGATTAAAGTTGGAACACTTTGTAAGTCATTGCGTGTGGTTCA

CTTGCTTCTGCTTGCAGTGATCTCTGTAACTTTTGTTTATTGAATGTGGT

CTAATAACTACAGGGGTCTCTATTTTGTTTCCTCCAATTTTTACCCAATT

AGATATGCTATCTATTTCACAAGTTTGGTCCACTITAGTTTGGGTTACTC

NGGCCGCGAACCACGCCGGGCGTTNCTTCTGCGGCTTGAAGCNGTACTGA

TGGTCCCAGGTTTGGTCCTTTAGTGGGGGGTAATGCGCGCCTGGGGACAC

TGGCTCCNNNCNCCGTTCCTCGGGAAGNGTTCGGCCAATCCCATAGNCGG

GAAGT

>E:\AπalyzedData/50513_2_2_Q l_Kubota_Hp 14_02Run0 l_Cp312_MD1/Q l_Kubota_Hp 14

_02_39G05.F.esd 965 MegaBACE

GAGCCAAGCAGTGGTACACGCATNGTACGCCCTCGAGCGGCCGCCAGGCA

GGTACTGTGCAATGTCTGACTCCTACGATGCACATGAATACCTTGCACTA

TTGAGAGATATTAATGATTATGGAATGAAATATGGACTACATGGCTTTGA

ATAATTGCATCTGAACATGGCAGCACTGTGAACATCTTTCTGACATCAGC

AAGTAATTTGAAAAACTTTAAAACCTTTAAAAAAATCGTCCAGAACTAAT

CTCGAAACCTTTTTTATCTAGTATATGGCAAGCAAAATACAAAAAGGCAC

ACTGGCCTTCTCTGTATCATGTAATTCCTTTAATAACAGACTTTCAAATG

AATTAAATCTCCTTAATAAAAAAGCATGTCTTCATATACTCCACATGTGG

TTGATGAACACCGCATCCAGTCAACCAGATGGGCCTCAAAGTAAAAGAAT

TTCCTATAAGTAGCATAATATTTTTTACCAAACTGATTCCCCATTTTCAC

AATTACTGACAGCCATGGGGTAAATGTGCCATTTAGAACACCAGATACCA

CACTTTGCAGCACAAAAATGCTTTTGTCTACACCATCCTGAGATTACACA

CAAAAACTAAGCCACTCACTTGCTTAGTTGGATGTAGAAGAAGAAGAAAA AAAAATCCTAATGTATTTGTGTGTGTGCATGTGTTTCACATGTGAATGTA

CTCGGCGGGACCANGCGGGCGTACTCTTGTCGGTCTGGAGCNGTACTGGT

TGGGTACCCCCGNTTTTGGTCCCCCTTTTATGGAGGGGTTAAATNGGNGC

GCTGGGGGGTACACGGGGCAGGCGTTCCTGGNGNGAATGGTTTCCGGGTC

AAATTCCCGATNGAGGCNGGGGCGCTAAGTAACCGGGGCCCTNTGTTCTT

CTCTCCTCCCCTNACCTCNTGTGCGCCACNGGCGNGTTCGGAACATTGCG

TTATAATCCGGAGTG

>E:\AnalyzedData/50513_2_2_Ql_Kubota_Hpl4_02Run01_Cp312_MDl/Ql_Kubota_Hpl4

_02_47G06.F.esd 1066 MegaBACE

GATGAGCTCAAGCAGTGGTACAACGCAGATGTACGCCCTCGAGCGGCCGC

CAGGCAGGTACCATGTGCGTCCACCTCGGTCTGCACTTGGGGACTGGAGG

ACAGATGCAGGAGCTGAGGAGAGTAAGATCAGGAATACAGGGTGAGAAGG

ACTCGCTAGTAACCATGCATGACGTCTTAGATGCACAGTGGCTCTTTGAA

AACCATGGCGACGATTCTTACCTCCGAAGAGCAGTGAAGCCCTTGGAAGC

CATGCTCACCGCTCACAAGAGAATCATCATGAAGGATTCGGCAGTCAACG

CAGTTTGTTACGGAGCCAAGATCATGTTGCCTGGCGTGCTGAGATACGAA

AACGGCATTGAGCTGAATGAGGAGATTGTAGTTTGCACCACCAAGGGAGA

AGCCATTTGTATTGCTCTCGCCCTCATGACCACCTCCACCATGTCCTCCT

GCGATCACGGCGTTGTGGCCAAAATCAAGAGAGTGATCATGGAGAGAGAT

ACATACCCGCGCAAGTGGGGCCTGGGACCAAAGGCAACTATGAAGAAGAC

CATGATCAAGGATGGTCTTCTTGATAAGCACGGAAAACCAAATGAAAACA

CCCCGTCAGATTGGTTGCACAATTATGTCGACTACAGAACGGTGAACAAA

GGAACCTGTAGACAGTTTTGCAGCAGCTACACCTACCGTTGAAGCTACGC

CAACAGTAGAACCCAAGAAGAGAAAGTTAGTGAAGGCTCCAAAAAGGCTC

AGCAGCAGCGGGGAAACTCCCTGCGGTTAGAATGAAAGCCACAATTTCAG

GCAAGGAACCGCCTGGCGGGGGTTACNTCGGCCGGACCAGGGGCGTCTTT

GTCGGCTTGACCGTATCGTTGCCCCGTTTTGGTCCNTTTCNTCGTGCNCT

TTTCTCCGCTTGTCGTTCACTGGCAGTCTCCGTGGAATGTTCGGCCACAN

TCCACANCGGGGATTTGTAGCGGGTTGTGGACCACATTGTGGCGGCGCTC

TCGNNGCTGTGTGTAAGACGCAGGTTGTGCTTCTTCTNTCTGTTNCNTCN

TTGNCCGTGCGCGCCT

>E:\AnalyzedData/50513_2_2_Ql_Kubota_Hpl4_02Run01_Cp312_MDl/Ql_Kubota_Hρl4

_02_56H07.F.esd 691 MegaBACE

GATGGAGCTCCAAAGCAGTGGTACACGCAGTAGTACGCCCTCGAGCGGCC

GCCAGGCAGGTACCTTGTTTAAGAGATAGAAAAACAACAGTAATATAAAT

ACCAAATAAAAGGAAAAATTCAGAAGTAAATGGCAATGAAGATGACAAAG

ATCTAAAGAAAATGACTACAATAACTTGATGGTTTATAATGCTTCCTTTT

CTCCATACAGAGTTGGATAATGCCAAAAATACGGCTAGATATAGATATGC

TACCAACAACAGTACGCAAAAGGCACCATCATTACACAATCTCAAACAAG

AGACAAAAAATAATAATTTACTGACCTGCTATCACACAAAAGTATTAGAT

ATAAACTGTATTATGCATGTATACTTTGGCACACTTAAATGTCACCCAGC

ACAGTCTCCATTCTGCATTCACGTCCTAGCCACGTATTTGACGAGACTGG

GGGATATGACGTAACCAGGGGGTTCGATCGTGTTGACCATGTGATCCAAC

ACGCGGTGGAAAGGCACATACACGGAACACCTGACGTCGCTGAGGGAGTT

TGCGAGCTTGGCTGCACTCGCGGTTGGGAGGCTCATGGCGGAAAAGTGGA

CAGGCTCAGGAGGGTCGTAGAGGCGGTAGAAATCGGGAGCAAAGCGGCGT

AGCTCGCTGACGTAACCCGCGTACCTCGGCCGCGACCACGC

>E:\AnalyzedData/50513_2_2_Ql_Kubota_Hpl4_02Run01_Cp312_MDl/Ql_Kubota_Hpl4

_02_82Bll.F.esd 788 MegaBACE AATGGACAAAAAAACGAATGCAGAAAAAAAANGGAAGGAGAATTGGAAGG

CTCNAAAAGCCAAGCTTGGGTAACAACGCTAGNAATGTACGCCCCTTCCG

AATGGCCTGGGGGCCCCTGGCCAGGNGGCATGGTACATGGGTGNCACGCT

CAAGATCTCAGGAACCCCCTTTCCGCCAAAAAGGGGGGGGGGACAAAAAC

AAAANGGCCCCAAATTTCCCCCGGCCCAACCCCCNGGGGGGGGGGAAAAA

CGGGGTGTGGGAAACCCCCTTTTCCCAAAAACTGGTGTGCACTAGTTCCT

CAAACCCCTGCTTCCTTTCCCCTTTTTTTGAACGATAGTGTGAGCCGGAG

TTGAACAACCAAACCCTTCCAAACCCCAAATTTTTGGGGCTGGGGGGGCC

CTTTTTTTTCCNGGGTTGAACTGGTCCTGGCCCCTTTCTCCNCCCAAAAC

CCCTTGGGGCAACGGTTGGTGGGCCCCACACGGGCAAAACACCCGGCGAA

CAACAACAANCACAAACAAACCCGCCCCCCTGTTTTTTTTTAAATAAAAC

CCCCCCGTGTGGGTGAAAAAAAACCCCNCAAATATTTTTCTCCCCTGGGN

GCAAACACACTACACAACAACTTGGCTTGGCCCCCCCCCAAATCTNGGCA

AAAAACCCCCTCATAAAAAAAAACTCACAAGTTTTTAAAACCCGAACACC

ACCCATATGGACCCAACNACCGCGCACNCGGCGAACAACATTTCCCCTCG

GCCCCCCTAGGTCTGGGCCCACAATCGGNTGGGGCAAG

>E:\AnalyzedData/50513_2_2_Ql_Kubota_Hpl4_02Run01_Cp312_MDl/Ql_Kubota_Hpl4

_02_84Dl l.F.esd 760 MegaBACE

GATGAGCTCAAAGCAGTGGTACACGCATAGTACGCCCTCGAGCGGCCGCC

AGGCAGGTACCTCATCAGGTCAAAGGTCAACCGGTCATTTAGCCGGAAGG

ACGTCGAGGCGAAAACAGATATTTGCATTACAGACACTTGGCCTTGCGAT

ACCATTGAAAAGTTGTTGTCAGGGGATAAGTGAACAAACCAAGGTCTTAG

CAGAGAATCCTGAGGCTTGGAATGACAGCCAGTTTAGCCAGCTGGAAATT

CAGAGCAAAAACCTTACTGAAGCAGTGGAGGATGCAGAAAAGGATGATTT

TAAAGAGGAGGAAACAAAGGAAAGAATAAATGACGTGAAACAGGTAGTCC

AAGAAGGGAGGCGAGTGGCTAACGAACAGGTCAAAAGTTTCAAAGCAGCG

GAACGAGACAGTATCATCTTTACGTCGGTGCTGGGGACTGTGGGCGGCCT

CATGGTCGTGGGTCTGCTGGGCATCGTCATCTTCGCCGTCTTCAAAGGCA

AGAAGGTCTCCAAGAAGCCGGGAAACAAGGGCAATAATCAGGCTCCCGAA

GAAAAAATCCCTGTCGGCGACTTTATTCGCGCCCACGACAACCCTCCGCC

CACGAGGATTTCCCCCGAGGTTAGGAAGGACCCTTATGCCCGCGACGACT

ATGACACGCCCTACGCACCAGATGCCCCTGCGAATCGCGACCCTTACGCC

CGTGGGAGGTCACCGCGGAGGCTCGCTCGCATTACTCCCAGGGCAGGGTC

NCAGGGGGGT

>E:\AnalyzedData/50513_2_2_Ql_Kubota_Hpl4_02Run01_Cp312_MDl/Ql_Kubota_Hpl4

_02_85El l.F.esd 1474 MegaBACE

TGGAGCTCAAAGCAGTGGTAACAACGCAGTAGTACGCCCGTCTGGAGTCG

GGCGGCCGAGGTCACGNGGTGACGGAAGTTTGTATGGCATTCCGCCACTA

TCAACCTGTAACAAGTTATGCGCTTGTGCTCAAACCTNCCACATCCATCG

ATCCACACCATCCATAGCTACCACATCTCAGTGAGAGCAGTCCTGAACAG

CGCTCGCTCTTCTCGGCACTTTCTCACGCACATGGTCTTTAACTCTCATC

TCGCGTCAGCCGGGACTATCGCTCGAATGNGTATTCGCTCCCGACCGTAT

AATCCTTGTATCGGAATACGCGGCATGACCGAGAATCGAACAAATANGTA

GCACTCCCTCGTCCAACATGAGAAGTGAGTGACTATGCGGCAAAATGTAA

AGGGCGAAAGCTAACAAATTAGGTATAGCTATTCTTGAACTAGTATAACA

AGTACGTGCAAAAATTATTGTAGTCAAATGACTTCTATCTGGTATCGGAT

CGCAAGCCCTTAAATTAAAGAATAGGATTTGGTGTTGCCCATACTCCCAA

AGATAAGATACAAAGAAACAGATAGAGAAAAAGACAAAGAAAGGAGATAG

CATCGAGCCACGGGCGCACCACTACCTNGCCTAGGGGGCCCGGTTACGTA TCGTGGGCTTCTGCTACATTTAGCATGGCGATACATCGTCTAGGTCAACT

CGCCGACTTAACCGTANCCACTAAAGTTGTATGTGTGAATCTCATAGTCT

GCAGGCGGACATGTATGNTTTGAAGTACCTTGGAGGTACAAGTGAAGACT

ATTCTATCGCTAGTCCNGGGCACAGTGGGTTTATTNCAGGGCTANTAGAC

CCNTTTCATGACTTTGCAAAACNCGGCGTNAGAAAGCAAATAAACAAGGT

AGGTAAAAGAANTATCGCGCGTGTTCGCCACATCATATAGGCGGATAGAA

TGGCCATTTNGCATGCGGACAAGGTATGAAATGTAGNCGGGTCTNAAGCT

ATACGAGCTTGTCGCCCGACGTTGTAAACCCAATATTCCGNGTTAGTATC

CCNTTGGNGGTCGATTCCCCATGGGGAATTATTACCCTCACCCTTTGGNG

TCTACACCCTTAGCAAAGCTGTAACTAGGAGTAGGGTTCCCTGGCGTGAG

TCCGGAGGGGCCTTCGGTTCTTTTGGTGCCCTTTGGCCCTCCCTTGGGCA

GGCGTCCGGCTTCCGGATGGGCCNGTCACGGTCCTGGTTCCGGGGCCTTT

GGAAGGGCCGGTAAACCTGGGTTTGTTACCCCCCNGCTTTTANGGGGCCC

CGTTANCCGGGGGGGTTTCCACCGGGCCAGCCNCCGTGGGGGTGTACTCC

TGGGGCTACACNTTGTTTTCCCCGTGTGCACTGTATGCGGGGGCAACACT

GTGCTTACTTCTCTGCTCTAGTGTNTCCCGGGGTGTCCAATGAGGACACC

CCCGGCGCGGATGTAANGAGACAC

>E:\AnalyzedData/50513_2_2_Q l_Kubota_Hpl 4_02Run0 l_Cp312_MD1/Q l_Kubota_Hp 14

_02_93E12.F.esd 1592 MegaBACE

GAATGGAGCCCCAAAGCAGTGGTAACAACGCCAGAAGTACGCCCGTCGAT

GGCGTCGGCGGCCCGGGAGGATAGCCATACAGACAGCGTNGCCTNACAAN

GACCATTTGAGAGAGGACTCGACAAGAACATTTCTCGTAAGAACAACGAC

AGATCCACATGAGTTGTTATCTCTGCTANTAAACACCGCATAGGTCCGCG

TGTCTATAGCGATAGCCGACATACATATAGCTTTCGTAGCGTATAGTTCT

CTCTATTTCTCGATCTTGACCCGGAATTATGGGGAGATANGCATATCTCG

TCTCTAGCGCGAATCGACTTGGCGCTCTATCTCNGCCGTATCTAGAACAT

AATTGTAACTCTGGAGAGAGAGATAGTTTGTAGTACTCATNGGGCCTGTG

GTTTTCTAGTATGAGGGCTCCCCATTCGCATCTTCCGAGCCTACTGAGCT

AGCCAGCACCAACTGTGTGATAGATCTCGCGCCGTCCCAACTATCTCCAA

CGCTAGGTGTTTGCCAACCATCTCTTACACTTCTACATATACTATCATAA

GAGGCCAGGGTTCCGTAGACGTTTCTNAATTTAGTAGAATGCCTAGGACC

ATTTTGAGTTCGGTTTTGCCACTATAATGAACCTANGTAACTAGCGAACA

AAAGAGAATTCNCCTTTGNGTTATATAAAGTTTATTACAGTACGTATTCT

TTTTCAACCTATNAGGAGGTAAATCCTTTAAATATAACCGTAAAAGTACC

TTGTGAAACTCAGGNNGAAAGCCTAAAGACCCCTCAAATATANTCCGGTG

AAACGAACGCGTAAACTATTTATTGGGGGGATGAGTCCGANATAAACTAA

GACATTTTCCAATTCTCCGATTGGTTTTTTAAATCCCCTTAGGGGACCAA

TTAAAGTNTGGAAGCAAAATCTTATTGTCNCCNTCATACTTTTTTCCCCT

TGAAGGTNGCGATGTGGGTGGNCCTTATAGTTGTTANGGCGTTTGAGTGT

CGCCCCCCANGGGTTCCTCTTTTTCTGATACTCCTTTTTGGTGGTCACCC

CCCTGGCGCCTTAATCNTTGGGTTGGTATTGAGCACCCGCAAGGAAACCC

TTTTTACCCTTATTNCCTCTGTGGGGGGNAGGTGTTAAACCCCCTTTGGG

CCCCCCCTTTGGTGGGCCGGGGGGGCGTCTGGGGCCNTTTCCGCGGAGGG

GGCNGGGCCCGGGGTTTAAAACCTNTCATTCCCGNTGNGTTTGCCNCGGG

CGTCCCGGTTTTTGGGCACAGTCCCCCGGGTTTGGACCCCTGGGANCCCT

TTTGGTGGGGTAACCCCCCCCTGATTAGATCCNCTTTGGTTATTTTTGGG

CGNTTTTCCCCCCCCCTTTAATNGTTACAGTTTTTTTGGCGACAGGGTGG

GCGGGCTTCTCCCAACTATTTTTTAGGGGCCTGTGNCCCAAGGCCTGTGT

GTTGTGTGTGCACCTGTGTTTTATAGGTCTTCTCCCCCNTTCCGTGTGTT TTGCCCCGAATTTTGANTNACCTTTGTGCCNTATTTTGGCGCCTTTCGGT

TNNGTTGGNGCACTACAGCAGTTTTTGGGCTCCCTAANTTCG

>E:\AnalyzedData/50513_2_2_Ql_Kubota_Hpl4_02Run01_Cp312_MDl/Ql_Kubota_Hpl4

_02_94F12.F.esd 532MegaBACE

GAATGGAGCTCAAAGCAGTGGTACAACGCAGTTAGTACGCCCTCGAGCGG

CCGCCAGGCAGGTACCAGTCATGCCACAATCATTGCAGGTCATTACGAAG

CCAAGTTTTTTTTTTTTTCTTCTTTTTTTTTCTCTCTAGCAAAATTCTAC

TTCCTTGTAATGCAATGAGCTTTGTGGGAGATACATAAGGGGAAGCCCCC

AACCAGTGTAGTGCAGTGAGAAGCAGCAAATGCCCCTACACCCCTTTCCA

TGGTAATGTTTTGTAGCCTCGTTTATAAAAAAGTCGTGGTCTTCACATGG

AACTCTTATGTGAGTGTGAGGTGAGACGAGTCGAGGCTCATTGCAAATCC

CATCGCACGTTTCGGTTGGGGTGCGACATGGCTAGCAGGGAGGAGTGAGT

GCAGAGTGGCTTITTGAGTTGTATAATTTTTGAACAATGTTTAATTGTAT

CTGTTCACTATATATTTATTATCTTTATTATCATCATTATAATGATTATT

ATTTAAAAGTATTTGTGACAAAGAAATNGTAA

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_Cp312_MDl/Ql_Kubota_Hpl4

_01_A19_289.F.esd 1054 MegaBACE

GATGGAGCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGC

CGAGGTACATGTCCTTCTGTGATCAATGTAATCAGCTTAGTGCATCAGCT

GCAAGGGCCCAGCAACCAAGTCACTTCCACATAAGGCTGGGGTAATGTTC

GCTCCACTGGGTTGGTAGATACTTTACCTCTTTATCACATCTATTCAGGA

ACTCCAGCTAGAGGGGCACCGGGTGGGGCAGGCGTGGGCGAGTTCATTTG

TCGGCCCTGTTCCTTAACAATAGGGTAGAAGTATGGGTGTTCCATGGCTT

CGTGGGCTGTCAGGCGCTCTTGGTGATCATAACGCAGTAATTTGTCCAAA

AGGTCTAGTGCTTCAGGAGAAACTAGATGCTGATTTTCACTATGGACAAA

CCTTTCCCACCGCTTCCGGGAATGCCTTCCCAGAATGTCATTGAACCTAG

GATCTAGCTCAATTTGATATTTCTCAACATATTCATAAAGTTCTTCGGTC

CCCAAGACCTTTGCAATGCGCACCAATTGGTCATAATTGTCATGCCCATG

GAAACAATGGTTCCTTGCGGAAGATCATGCTCGCTAGCATGCAGCCAAGT

GACCACATGTCCAATGAGTAATCATACATCTGATAATCTACAAGAAGTTC

AGGGCCCTTGAAGTATCTGGATGCCACCCGGACATTGTATTCCTGGCCTG

GGTGATAGAATTCTGCCAAGGCCCCAATCAATGAGGCGTAATTTCTGTTC

TCATGGTCTATCATGACTTTTGAGGTTTGCCTCTNCGGATGCTTATTTCC

ATNCTGGGCACGGTATCTATGCNTTAANGGAGCTCGTAACNTGGCCTGGG

GGCCGATCCAANGGCCGATAATCCGGCCGTTGAAACCGNACNGATGNACC

AGTTTGGTCCCTGAGAANNGCATCACGCGCCCCTTGGGTGACACAGGCAA

AACATCCGNGGAGAAAATTCCGCGCAATCCAANANAGCGGGGAAAAGTAA

CGGGGCAAAGAGACACAAATGTGGCGCCNCCACAGAAANGTGCGAGAAAA

ACCG

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_Cp312_MDl/Ql_Kubota_Hpl4

_01_A23_353.F.esd 1811 MegaBACE

GGATGGCCCCGGACAGACGAGTGAGTAACAAATNGCAAGTAGGTGCGCCA

CTTCGCAACGCGCGAGCCAGCTCTAATGTCGAGGTGTACNTATAAANCCA

TAACAAGCGACCACACTACCTTTTAAATATGTTACACTTATTCTTGCTCA

CTTGACTTNATTACTACTCTCTCACACANTCTAAGTCGGAATACTTTTGG

TTAATTTCNCAGTGAATAAGGCACAATCACTNCAACACGATGNACGCTCC

TTGCATACCGGATATTGAAGACAAGCAGCTCTAGTTATTTATATATCGTA

TGCAGGAAGCGATCCTAATCGATTTGCATGGNAACTTTTGGGTCCTCATC

TACTCGCGAAAATTCAACCAAAGCTATTACTCGATTCCGAGATGTGGGTG AGCGATTAATTATATGTGNGANCCAGAGTANAACGAATAAACTAAAACCT

AATATAATGATAGGTTCCCTGTTCGTTGTTATCTTAACCCCGTAGNNACT

CNGTGCGTGGCGGTNATCGAATGTGGGCGNTAACTTCTTGGACCGTTCTT

TTTGNTCACCTGGGCGTTCGGNAAGCCNACCCGACCTGTCCGGCCTACCT

TAGTTTGGTGGGTACCTTCCAATTTAACTGAGCGGCGGGTCCGGCTTCCT

NGTGGGCGGCCCGTTTTCCGGATGCCGGGTTACCCTNGGCGTTTGCCGGT

TNAGGCACCCCNTAGNGGCGTAATAATGAGGGCCCCCCTCGCGGTCTGGT

TAGGATCGCGCCCGGAGGGCGTTTTGAAAATTTTCCGGTCGCGTCCGGNC

TTCTNAGGGGGCAGGTTATAACTNGCGCTCTGNGGGTTTCCGGGTCCCTG

AGGCGTTCTTGGTGGCCGGGTCTNTCGNTTANGGTTCGGGAGAAAAATTA

ACAGGGGGGTCTCCCCNNNNCCNGGGCCCTTGCCAATTTCCCGGGTTGCT

TCCGTTTTTCTCTCCCCATAANTCCAAAGTGTAATTTCGGCACCAGGCCC

ACAGGGCCGTGTAAGGGCCCTAATTTGGGGGGCCCGGGTTTGGNTGGGAG

GTGAAACCGGCGTGGGCCCCTCGGTTNGGCCAACCTTCCGGGTTTCGGAG

ACTGNTAGGNNAGGCGTGTCTGGCCCTTTCCACGCCGAGTTTTGGNCAGT

GTGTTGGCGGTTTTAGCCGGGCCCTTCCAAAGCTGGGGCGCGCCCTGNCG

GGTTTTTACCCAACGTGTTTTTCGGGGCCGCGGAGGAAGGGCTCCCCTCA

GNTTAGAGGGCTTGGGGCCCCCCCTGGGGCTTCCCGCTTGTGTTTTCCGA

GGGCTTTGTGTATATCACTTTTATGGGGTCCATTAATAACGCGCANCTTC

GCCCCCTGTACCCGGGGGCCAACGGAAACAGGGGGCCACACCGGGGGCTC

TCGTGTGGGGCCTNTTTTAATTTTCCCGCGGCGTGGTCGCGCCTTTGGAT

TTTTTACCTCGTGNAGAATTTTCCCCGGTTAGCGGGGTGGCTTCCCTCCA

CCCCCCTGTGGTANACTNGACCCGGGGGGTTTTGTCCCCGCGCGCGAGNN

CTTGTGGTGAGACCCCTTGGGGGTCTCCCTGGTGTGACCTTGGGTGGGGA

GAGAGCCCCGATACTGNCCAANGGCGCCTGCCCTTTTCCAAGGGNTCCTG

TTNNCTCCTCTTGTCCCAATTTGNTTGAGGGCGCGGTTGGGTAANTNTGG

CCTCTGCCGATCTGGTTTTNNCCCTTTGGGGGGGGGACCCCACACTTCCG

GGGGGCCCGTTTTTAATTNCTCGGGGCACCNGGGATTTATGTGGAACNCG

GCTTCTCTTTT

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_C_P312_MDl/Ql_Kubota_Hpl4

_01_El l_165.F.esd 1100 MegaBACE

GAGAGCCAAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCGCCAGG

CAGGTACGCGGGGGCCAGGCCGTTGTCAATGGACAGTTTAAGGAAATCTC

TCTTGAGGACTACAAAGGCAAATATGTTGTGCTCTTCTTCTATCCTCTAG

ACTTCACATTTGTTTGCCCTACTGAACTGATTGCCTTCAGTGAAAAAACT

GAAGCCTTCAAGGAACTTAATTGTGAGGTTATTGGGGTGTCGACTGACTC

TCATTTCTCACACTTGGCATGGAATAACATGCCACGAAAGCAAGGGGGTC

TGGGTGGCTTGAAATACCCCCTACTTGCTGATTTCAACAAGACCATCTCA

AGAGACTATGGCGTTCTTCTTGAGGATGCAGGCATTGCTCTCCGTGGCCT

CTTCTTGATTGATCCCGAGGGTGTCTTGAAGCACATGAGTGTAAATGACT

TGCCAGTAGGAAGGTCCGTAGAAGAAACCCTGAGATTGCTAAAGGCCTTC

CAGTTTGTGGCTGAGCATGGTGAAGTATGTCCAGCCAATTGGCAGCCAGA

GAGCCCAACCATCAAGCCTGACCCAGAAGCCTCTCTTGAATATTTTGAAA

AGGTCAACTAAAAATAGCAATGAAAATAAAACCTTCAAGTAGGGGGGCTA

GCATCGAGAATGTCATTGAAAGAATAAGGCAGCTGTATATGTCATATGAT

GTGTGTCTGGGCATTTGCATTGTATTGTTCTCAATGATGGTTTCCCTTGT

ATAGGAAAGAGATTAATTGCTTGAGTCATCAAAGTAATTTTCATTTGTAA

GGTNTTGACAAATAACCAATATAATCTAAAAAAAAAAAAAAAGTCCCCGG

CGGGAACCAGCCTGGGCGTTACTTNGGTCGGCTGAGTACGATTGGNCCCA CGCTTTTGTCCTTTAGGAGGGTANGGGCCTGGGGTATCAGCAAACGNCCG GTGAAATGGATTTCGCGCAATCCACAAAAGAACGGGAAAGTTACACGGGG GGCTCAAAGAACCACCATAGTGGGCAACCCTGGAACACGCTAATGNACAG CGAGAGNNTGNTNCCCTTCGNTNGTGTCTCCNCTCAANCTNCACCNCGCC

>E:\AnalyzedData/50425_2_2_Q l_Kubota_Hp 14_01Run01_Cp312_MD 1/Q l_Kubota_Hp 14

_01_E15_229.F.esd 1648 MegaBACE

GAGCNGGGGCGCCCGACCGGACAGTGCGCTAACAATGCGTTCATGAGATA

GCGACCCGCCNAATATTACCGAACGCNGTCAACTTATANTGAGACAGGTC

GTTNGTGCNCGAGCTCANGCTAGCGTGTATCACTAATGTNAATTGTACCA

GAGNACTCAGTTTACTCATNGGGAAAGTGGTATATCCCCAGTTCTAAGTT

CGACTTTCATCATAATCTACCTGGTACTAGCATTAAGAATTGGAATAAAT

CCTCCNGTGTACGTGTGNTAAGTTAATGAAATTGGNACTCCACCTACTCA

TGTTCGTATATAAAAAACTAGATAGATTACCATTTGTACGGCGACATATA

CGAGTGAATATGTATATTTGGAATCAGTAGCATAATTCTATGTAGTATTT

ATGTACGCATGTGATCCATATCTCATGAGTCAATACGCAATAGACATATA

TACTATATAGACATACAATAGAAATAAAAGTTACTCACGAGANAATCATT

TGATTTAGTACGGACACTCTGTAGAGGACATCACCAAGTAGNTTTCCTAC

GGCAAAACAAGAAAAAGACACTAGTGGTATATATACTACCTACGTTTATC

AAAGCCCCTTAAGCTTCCGGTGCAACGCTCTATAAATGGATATCGACAGG

ACTGTATGTTTCCTGGTNCCTAAATAGACCCTATCGGAGTTTTGTTTCGT

CCGTGTNTCNNTTCCCACCTTCTACCCCTTTCTTCGGCTCTCTAGGGAGC

CCTAACTTGTTATTTGTTAACCCCTNGGGCCCCCCTTTGTGTCCGGTGCC

TTCCNAGGTCCGTCCCCGATNGGGTGCCTGGTTTACCNTCTCCTTTTGGT

ACCTGCTTCCAATCTTTGCAAAAGGCCCCTGTNTCAATNCCGTTGGGCCA

CATTTGGGAGGGTTCCACCCCGCCTTAATGGGCTTTTATTCTCTNTGGGG

TTTCTCCCCCCCTCTCGGTTNAGTTCCTTGAGGTTTCCGCGCAGCGGTTG

CCGGTTAATATTGACGGAGTTGCTCTAGGTNCAGCGCCCTGCCCTTTTTT

GGTTGCCCCGGGGTTTACACTACTTNGCCACAAGTTTCGGGGGTTTCCCA

CTTTTGAAAGTCCGTTGGGTTAATCTNCCCCCCATTGGCTTTGTCTTGGG

AAGGATAATCCTCCGGCGTTATTTATTTACCGCCTGGGGCCATNGCCGGA

AGGCTTATAATTNTTTCCGCCGGACCGAAACCCGNAACATTCTAAGNTTA

GACCTGGGTAAAGGCCCCCCGCGGGGTGGGAAGGGTCCCCCAATTTAAAA

GNACCGGTTTGTGGTTTTAAAACACACCGTGTTCCNCCTTTGTGGGTGGG

GGAGNGGTTTAGGAGCTCCCCCCGTNTTGAAAAAATTTTGCCAACCGTGT

TTGTGGTAAACGNCCCNCTTNTAAAAAAATATGTTGTTCCCCCAACCCAA

TTTTTCTTAAAAATNTCTTTTTGGACCCGGTTTTATATAGGGCCCCGGCG

CCGTTTCCAAACCTTTTTGGGGTCCCCCCCCGAGGGGGTTTTGTTTTCCC

CCCCCAAAGAGCTCCCCCCCTGGAGGGGGGAAAAATAATAGANCGCCCTC

CCTTGGATATAAAACCACTTGGTTATAAGGGNGGCTCCTCAGAAAAGG

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_Cp312_MDl/Ql_Kubota_Hpl4

_01_G23_359.F.esd 1104 MegaBACE

GATGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAG

GCAGGTACACTTGCTTGCACTTGGCAGTTTCATTTCCAGAGCGCACACCA

ACTACTTTCCCGTCCTCCACCACAATTTCGTCGATGGGCTTGTCAAGCAT

GTATGTGCCTCCATAGATAGCAGACAACCTAGCAAAGCCCTGGGGCAGCT

CACCCAGCCCATACAGAGGATAGAGGTAAGGAGACTTTCCGTAGCGAGCG

AGAGAGTCACTGTAAAGCTTAATTCTCTTCAGGGCTTTCCCCGGCGGGCC

AATTGAGGTATTCGTCATCCCTATATAGAGCAAGAGCGTGACCTGTGAAA TCAATGGTGTTCTTGTCGAGGTTAAAGTGGGTGAACACCGCATTCATGGC

GGTGGTTTTGGGGTCGAAGCCAGGCATGGCCTTCCATGTGGCTGGGTCAT

CGTCACGGTAGTCCTGGGCGAACACCAGGAAGTTCTTGAACCTTCGCTTT

TCAAACATGCCCATCAGATCTGAGGTAAGAGCCTCCTTCTCATCCGCAGG

GACCTTGGAGATTTTGTTTCCCTTGTACTACTTGGATGGCAACCTGAAAA

TCACCCAGAGCAGTGCCATCATCCGTCACATTGCTCGCCAGCACGATTTG

TGTGGCAAGACAGAGGAGGAGAAGGTTCGTGTGGACATCCTTGAAAAACC

AGGCTGTTGACTTCCGCAATGGATTTACACGACTCTGTTATAGAGAATAT

GACACCCAGAAGGATGCTTACTTAGAGGCTCTGCCAAAAAACACTTAAGC

TCTTAACTCCGGATTTCCTTTGGGAAACGCAAATGGTTTGNTGGGGACNC

ATTTTAATCTACGTGGACTTTATCCTGGTTTGGACTCTTGATTGNACTTT

CGTACTTNGGGGACCNAGCGTTNGNNTGTTCTCTTTCTCTCTTAGCGTTC

TAGTGTGCGNTTGTGTCCCTNAANGGGTNTGCGGCCTGGGTTTCGGNCAG

CTTCCTGTGGATTTCTCGCNTCACCAAAGGGGGGCAGTTCTGGGNTGATG

ACCCATTGTGCGTCGCGTCGCAACGCGGTTNTCTCTTCCCCTCNCGNCCG

NCCG

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_Cp312_MDl/Ql_Kubota_Hpl4

_01_K21_331.F.esd 1004 MegaBACE

TGGATGGTAGCTCCAAAGCAGTGAGTAACAACCGGCCAGTAGAATAACGC

CCNAGCGCGANGTGCGGCCCGGCNAGCGACGAGTGACTTTACGGAAGTGG

TTTTATTGGCGTCCTCACTCAACCAACACGAGCGTGTAATCTACAAGATA

TGTGATGCGTCCCAGAGTGTCACCGCTTCCTACGTTCCCAGACTCAATTG

TCAGTGACATTGCTCAAGAGTGATGTNGTGTGACGCGATGTGTTGTCATT

CGCNCTACTGATGAAACCTGTGTGCGTGTACGTATATANGTGAGTAGCCA

GAATGTCTCGCNGTNTNACATATATATGTCNCCNCNTGTCTTACTGAATA

TGNGTCGGAGTCAAAGCGACCTCTGGTCCACCAATNCAATCACTTTCGAA

CAAGTTCCATTAGCAAATTTGATTCCTTGTTGAGAAATGCAGAGCGGTAC

ACCTTTGAGGNTGCATAATTAANTTTGGAAATCGCTCGCGTTTGTGGTAT

AAAGCCACATAAAACGTTGGCTANGCTTATCGCCCTCCTGTTGGGCCTGC

GGCAGCCTCAGCATTTGGCCGCGTATCACNCTCTGAGCGTTAGTGTCACC

CCACACTTGGCTATTTTGTGNANGNCGCTCTCCCAAAATATTCTCGTGCC

GNTTTATTGATGTNTAGCGATGTCGTGGATATANTAAAACAAAGCGCCGC

CGCGCNGCCCCTCCCAATTGTTTGTGGCGCCGCGNTGGACGGGGAAAATT

AAGNAAAGCAAAGGGGCGATTCGCATTANAGGACGANGGTTGCTAGGTGG

AGAAAAATAAAATCCGTCCTTNGTTCTGTGGAAACCCCGNNGAAGGGGGT

TTTGATAATACTAGNCGNTTATTGAATGGAAACACGGGGATACTAANACN

NCACTCATTATGTAGAGGGGCAGCACCAATGATAGNTAGAGTACTGANTT

AAGGAAACGGGAGACANAATAAAAACAGGNACGCCAAGATACAAGAAGCG

GNGT

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_Cp312_MDl/Ql_Kubota_Hpl4

_01_M21_333.F.esd 992 MegaBACE

GANGGAGCCAAGCAGTGGTACACGCAGAGTACGCXXXXXXXXXXXXXXXX xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTACATTCAATC

CTAAAGGTNTCTTTTGCAGTTACAACCTTGGGCTTCTTGGACCGTTCACC

ATGCTTAAATCCCGGCGGAGGCACCCTATTTTCANGGNACCGGGTCNANT

CCGGCACATCCCATAGTTGCCTGAGATCCCGGTCGGGTTGAANTCTTCAC

GACCGGAGGGGTGACCNTCGGGCCCTCNGTCNTCGTGTAGGG

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_Cp312_MDl/Q2_Kubota_Hpl4

_01_A06_081.F.esd 893 MegaBACE

GATGGAGCCAAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCC

AGGCAGGTACAAAGTGGACATTGACTCAAAGCTTGGCAAGAGCCAGGTCA

TCACTAAAACTACTCCAGCATCTCAATCAGGAGGGTATTATTGCAATGTG

TGTGACTGTGTTGTGAAGGATTCTATAAACTTCCTCGACCACATCAATGG

CAAAAAACATCAAAGAAATCTTGGGATGTCCAAGAGAGTTGAACGATCCT

CTCTGGACCAAGTGAAAAAACGTTTTGATATGAATAAGAAAAAATTAGAA

GAAAAGAAGAAAGACTATGATGTAGAACAGAGAATGCAAGAATTAAAGGA

AGAGGAGGAGAAGCTTAAAGAATATCGACGTGAGAAACGCAAGGAACGAA

AACGTAAGGCAGATTCCTCGATAGATGAGGACCAAGGATCTGGCATGGAC

CCAGAAATGGCAAAAATAATGGGTTTCTCTTCGTTTTCATCATTTAAGAA

ATGATATTATATTCATATTTATTTTCTACATTCAAGTGTTGTTTATAATT

ACTTTAAAGTCAATATACAATATTAACATGACTATATTGTAAGATGAGGT

TTTCTGTTATTTTCATGTTAATTGTTCAAGTTATGCAATTACTCTTTTCC

AAAGTGATAACTATAAAAAGTCATTTTANTGTGAAAGCAGGATGACCATT

GTCCCATTTCTTTTCTTTTGTTTTGCCCTGTATTACTTTATTTATAAGAA

TAATCTTTGAGCAAAAAAAAACAAAAAAGTCTCGGCGGACAGCTGGCGNA

ATCTGGTCGCTTGACGTACTGATGGTACCCAGTTTGGNCCCCTTAGAGGG

TANGGCGCTGGGGAANAGGGAAAGCCGGGGCTCTTCTCNNCNC

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_Cp312_MDl/Q2_Kubota_Hpl4

_01_C08_115.F.esd 1693 MegaBACE

TGAGCTCAAAGCAGTGAGTACACGCAGAGTACGCCCAGCGTGAGTCGCGG

GCCCGAGAGTACNCTTACANTTACCAATTCCATTATNAAAAATCCCACTT

TTAATATTAGAACNCTGGANTTGGTCAAATANTCTGGGAATGGGTTACAC

CTTATACTCTAATTATGTGTTCCATTAACTNGACCTTTTACTCGGGAAAT

GGTCCGNACCCGAAAGAGCCAGTTGGTTTAATTTACACATTATTCCAAGT

TTAACAAATCTCTAACTCTAACTTTGTTGACCCAAAACGGGTGGTTTGGC

CTTACCCTGNGCTCAAGATATAACTCGGGCGCCATTTTCTCTGGTTCCNA

AATATGTCGTGACTCTCCCTATGTTTACATCTGTGGCCCGGTATTCCCAG

ACCGCGTCTCTCTAAGTTTTTACCCCCCAAATGTGNAACCCTATTATAAC

CACAACTGTGGGNCTATTTGGGGTGTCCCNTGGTGTCCCCCCGAGGTAAC

TCCGTGCCCNCGTTGGGGCGGGGCGCGGTNCCAAGGGNGCCTGTTAAACC

TACTCGTGGGTCCCGNGCTCTGGGAAAGCGCNGTTACTCCGTGGTNTGGT

NTAAACCCNGAAGCTCTTTTATTGNGGTTCTCCCCCTCTTATATATAAAA

TNTGAGAGNGGTCGTTAAAAATTTAANGCACGGCCGGCCTAATGGGGCGN

ATTAATCTCCAANNTGGGTGTNACTATAACGNCTGTATGAGTNTACTCCC TCGTGGTTGNTTGAGAANAATTGGGTTATAATTCCGCGGTNCTACCACCA

AAATTTCCCCCAACCACAAAATATTAACAGAAGGCCCGCGGGGAGAACGG

CCTATTAAAAGTTGGTTTGTTTACAGAGCGGCCTCGTTGGGGNGGGTTAG

TCGCTCTTATAGTAGGANTGGTTAGGGACGTCCTTTAANTCCTCCAACTT

TTTAATATTTGTNGGGCTGTTTGAGGCCCGCCTTNCCTNGTTGGCCCCGC

GTTTTTACNCCCGTGTGCTGCGGGGGGGAAACACCNCNTTGTTCTTGNGT

CGCCGCGTNCGTGGTCTTATTTAATGGTAGGCTATCTTGCGGGGCCCAAC

AGCGCGGCCGGCCGGGGGAGCAGGGAGCGNCGGAGTAGCAGCGCCAATTA

NTNTGAGGGCGGCGCCCTATCACCAGCCGGGATTTCTCCCCGACGGCACA

CCAAATGNAGAACTTCGATGATTAGGCCACCTACGGCACCTTTCTCACAG

NACATTAAGGCGGGACAGACAGCGAGATTACACACAGCTCTCACCATCAC

ACAAAGGGGGGGAGAAAACAACGGGTNATTCACTCCCACNACANAATCAT

CCCGGNGGGGNAAATACGCCCGCGNAGGAACACAATTCTTTTTGTGACGC

AAGGGCGGCAAAGGNGGCCGAGAGGCACCAGAAAGAGCGCGGGGTAGGAG

GTTACCAATAGGGTCCGGCCCCTTAGGAATTCAANNAAGTNANATNGTAC

NCTTNATGTTNGTGTAGCACCCCGGTAAAAAACGGGGCTCGAGAGACACC

ACGGAGTAGGTACTAGCCGCAGAAGGACGTATCCATCAAGGAGGGCNGCA

ACAAAGTTATTGAGGAGACCGGAGAGTGCANAGCCCCCCTCTCTTCTTCC

TCNTTCTCCACNGTNGCNACACAATACTCTCGCTTCTTNTACT

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hρl4_01Run01_Cp312_MDl/Q2_Kubota_Hpl4

_01_G22_343.F.esd 1004 MegaBACE

GATGGAGCCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCC

AGGCAGGTACGCGGGCGAGTCGCCGTCGGAGCGTCCATCGAGGAACATCT

TTTTTTCGATCTCCTTCACCTAAACAAAATGTCTCCCAAAGTCCTCGTTG

GCGTGAAGAGGGTGATCGATTATGCCGTGAAGATTCGTGTCCGCCCCGAC

AAGCTGGGTGTTGTGACAGATGGCGTCAAGCACTCGATGAACCCTTTTGA

CGAGATTGCCATTGAAGAAGCAGTCCGTCTGAAGGGGAAGAAGATCGCCA

ACGTTTGCTTCTGAAATAAAGAAAACCGATGGTGAATTGGAGGTGACCCG

AGAAATTGATGGCGGGTTGGAGTCCATCAAGGTGAAGCTCCCCGCCGTTG

TTTCTGCTGATTTGCGACTTAACGAACCTCGCTATGCCACGCTCCCCAAC

ATTATGAAAGCCAAGAAGAAGAAGATTCAAAAGATGAAACCAGGCGACCT

TAGTGTGGATGTAACGCCTCACCTGGAAGTAGTTGAGGTGGTTGACCCCC

CCGTGAGAGGCAGGAATCAAGGTGGACGACGTAGACGGCCTCGTGACCAA

GCTCAAGGAAGCCGGACGCATCTGATGGGACTGTCATTATGAAATCGGAA

ATGGAATAAGATGTTGATTGAATTTACGGTGTTTGTATTAACTGTGAGTG

TGAGAATGAGTCACTTATCAATGCAATTTAACGTTCAACAGTTCTTTATA

CATTCTCGGGAAACCACAACTAACCAGATNTAACTTTTGGANTGTACTCG

GGCGCGACCACCNGGGCGTACTCCTGTCGCTGACGTACTGATGTNCCAGT

TGTCCTTGTGAGGTTATGNCGCCTGGGTATCAGGCCAANGGGCGGTCGCA

CNTTCCNNCCANNCCTTGGCGGCATATGTACCGGGGCNTGGNGCCACATT

GGTGGCCGCGTCTCGGAANTCTGCCTTTTTGACCCGGNNGGGGTGGNCCC

TCAC

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_Cp312_MDl/Q2_Kubota_Hpl4

_01_I02_025.F.esd 1720 MegaBACE

GCAATGGGGAGGCCCCAAGAGAGCCAAGGTNNAGNTGNGANCAAAANCGA

GCTATGTAGATACGGGCTCACGTTTTAATAGTACAGCAACTCAACATAAA

TTAAGGGTGCATGCGGAGGGGCTGAGGCTGTCACTCAGTTGGACCAAGGT

AGCAGAGAATAAAGTCATGATTTATGGGAGGTGCGGTTATGATTTTTATT

GATCTGAACATAGCTAGCATAGATTATGTTATAAGAGTGTGTGGTTATGC TATAAATTGTTAATCAAACAGAGGAAAAAAAtTACGTCCTGATTAAAGAG

CTACTGCTGAAGTTTGGATTATCTGAGCATGGTTAACTAGTTATTTAACG

AAACTCCCGNCACAGATATTTTGAATTATAGAGAGAGGAAATACTACTGC

CATAAAAAATCTATTCAACTTGAACTTGTTAGCACTTCGAGACACTGACT

GTAACCGACCGCCCTCGCGCGACTTGTTACTGTACTTAGTTGCGGGTGGC

ATATGCAAGACCGCTAATCATAGAATTTGGTACCCAAGCCTCTGATATAT

CGCTATCCCCCCTCTGTCAGGATCGCAAGGCGCGCTTTAAGTCTGGTCAG

CCGCTAGGTGCCCGTTCCAATTCAATCGGGTTACATGAAAGTCTGGTTAC

CACCCATTGCTTGNTAGAGAAATCCCGTATTATAACCTGGCATGCGAGCT

AAATTTCCGACCGACCGACATCAGTGACCGGTTAAGACTCCCGAGTGGAG

TCCCATAACAGACGTTTGGTTCATACACTGTCGCTTTGGTGGAGATAGTC

CCTGTTTAAATTGCACGGTGCGTAACGACCTTAAAAATCTTTCCCAACCA

ATCTAAAACTATTGAACCGGTATGGCCCCGGCGTGCAGCTAGGTCACACA

GGCTTGTTTACACAAGGCTCCTGGGGGGAGATAGCCCCTTGATACTTGGT

AGGCTCCGAAGGCCTTGACAAACTTTTCAAACTAGTAAATACTGTGGGCC

GATAATCTGGCCTGGCCCTGNCGAGCGTAGGCACGGCGGCCCGTGTTTTC

TCGACGGTTCCAGTGTTGAGAGGACCGCCTTGCTTTCTCCGGGCCCTATT

CCCTTGCCGGNCTTTCCAAACTTTGAACCTTTCCGTGGCTCCGACCAGGC

CTTCCGGCGGTTNCGGNTATTCCAGGGGGCTTGGGCTTGTGNCCGGGAAG

GTCCTGTGGTGNAGTTGCCAGGCCTTTCCTATTCTTCCGAATATGGCGCN

CTGCGGGTCACATAACTTAACCTGTGGGTTTTTACTTCCCCATGCCCAAG

CACACTGCCATGCGGGTGGAATTAAACCTNGCCCAGTGGGAANAAAGAAN

GCATTAGGTTTGAAGGTCCCAAAATATCCGGCCCCATGGCCAAGATAAAG

CGGCCCCAGGGGGCAAACTNCCGGTTAACAAGAAAAAGTNGGCCCCGGGC

GGGTATTGGCCTTGGGGCTGGTTTTATNTTCCCNATTTATTGGTCTTTCC

CGGAACCCCCCCCGCTTTTAGGAAAGGCATTCGCCAAGAAGAAATTCCGG

AAGGCTCTACAGGCTTCTCAAAAAAGGTNTTGGGGAAAAAAACCCCGNAA

AAAGGGGATCTCTTAGAGCNACATTCCAGGGGGTTTTCTCCCCCCTNGAA

GACTCCCTCTCGTTCCGGCACTTTCTNTGNTTACCCGCCCCTTGGCGTTT

AAACGGANAAACGTGNCGGC

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_Cp312_MDl/Q2_Kubota_Hpl4

_01_I04_057.F.esd 1043 MegaBACE

GATGGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCGCCAG

GCAGGTACGCAGATGAACTACCTAAACAAGGCTCTAGACATCTTCAACAC

ATCATTGGTGACCCCCATCTACTATGTGTTCTTCACTGCTTGTGTCATCC

TGTCATCAGCCATGCTATTCAAGGAGTGGAAAGGCCTTCCAGCCAAGGAT

GTGATTGGGGTCCTATCAGGATTCTTCACTGTTGTCATCGGCATCTTCAT

CTTGCACGCCTTTAAGGACATGGACATCACTTGGACAGGCATGTCGACCA

TGCTCATGGCCGGTGGAGTCAGTGAATCAAGGCGAAACAGCACAGAGGAC

ACAGGAAACGAAGTGACACCACTGTCAGAGGAGGAGGAGGGACTAATTAC

CTCCGAGCCTCTCTCATATGGCTCCAATAAGAGAGTTTACTAAAGCTTAG

GACAAAGCACAACATCATTCCTCTGCCTCGTTTCCATGTAATTCAATTAT

GCTCAATGATGACGACTGAAGTGCTAAAGATTTTTTTTTATTATTATTAT

TATTATTATTGTTATTACTGCTCTCGTTCAGAGTGCTTTGATAGGTTATG

GGTTTCTCACTTTTATACAGTAGAGGGAGAAGAAAGTTGTTTTNTCTCTG

AAGATGGAGGTTACNAATACGAATTTTACGTGATTAAAGTATTTTTGGTA

AACTACTTGAAAGTATACATCTGTGATGCATTCTTTTATATGAGAACCAA

ATGTCTTGCTCTCTTCCCTAAAAAATATGGTCACCNGGATCCGGAAATTT

AGCGCCTAAAACAAACCNGAATAGAAATTNCCGNGGGTTGCCATTAACTT ACCAACTTCCTCCGGGtTCAAGGGCTGGTTGTTCCTTCTCCTTTTTCCCT

GCCTNTTCNTCCNTCTNGCTCTCAGTTCTGCNGGCACCGGTCTTCTTGAC

TTGCCCCTTCCTAAGGTGCCNCTCANTCTTCTTTGTTGCCCCAGGGGGTG

GATTCTTCTCCCCCNCNCTCTCNTGCNNGNCCNCTCCNTGCNC

>E:\AnalyzedData/50425_2_2_Ql_Kubota_H_P14_01Run01_Cp312_MDl/Q2_Kubota_Hpl4

_01_I08_121.F.esd 2604 MegaBACE

TCTGTNTGNTGCGTTTGTTTGGTCCACAATAGGCTGGTTTCTTGGCCCTT

GCTGGNGGGANGTTATCCAACCCATGAAAATNTAATNCATAGCATAAGAA

TCAGNTAAGCGAANTGCATGCTGAGTGAACAAGCAAGGGACTAGTNCGTT

GTAGACGCTGATCGCGATGCGCGACTACGTCAAGAGTCGANGAACACAGT

ANGGTCAGCGCAGCATAGTAGGCACTGCACATCGCNAGGATAATGACANC

ATGAAGACGCCACACAGACGGACATACAGACGATGAGACAGNCAAGCATG

ATAACAACGGCAACNGCACGCAACGTACAACGAGAATGCGTACACANGCA

CAGTGCAATAATATCACAACACGCGAGTGGGCGGNGTAGCAGATGGNTGC

NGATCGGACCATAGAAGCACNGAAAACGCTNTGNCCGGNCGNCGANNAAC

ACATCNGGCAGAGTCAAGCACAGAANNTGACGCCTGACAGTATAAACGAA

AGGGCTGGCANACCNNAAATACAANTACCNAGGTGTAATANNAAGCCCCC

TCGCGAGCGTTNTANTCCGNTAATGCCCTCACTNGCCATGTGGCATCGCG

CAGCATANGNNTGATGTANCGACNCACCGTACCTGTCGACCGCCACACCA

CTNACGCCTAGCCTCAGCAAACGANACCTCTTTTNCGNNTNTNGATNAAC

CTCANTCNAATCACCAAGTGGCNTCNGTAGCANAGGTCCNCAGTATCTCT

TACTGNAGAACNACNCTNAATCTATTCCGTGCTGTGAGCAGTNNAANANG

GNATAGAAAGNATCNCCANCNAATGTNTNGNTAGNTCAGCATCGCCGTTC

NCAGAACNCGGGTTNTGATCTGTCCANATTGAGNATACGNTANACCCNCG

NANTNANATNTANTTCGGGACTAACGCGGTNCGAGCTNCCGGTGTCTNAN

GCAATNTCGTCCTGACCTGNCCGATGTACAGTNTAATNACGGCGTANGGC

NNTGTGAGACCCTACGCAAGTGCANACTCCNCTACTGTTTTTTGATGCNT

NACGTANGAGNGTTCTCCGTAGACACAGCTNNACNATAAGCTCCAACCTA

GGGTNCTACNCNAAGCTCTAACGTNANCGNAAATTCAGTCTCAGCACCAT

NACNGGGNNTGCGATACGACACAANAGNGCTTGGAAGNTTAATATTAGTN

ATATCCTCTAAGCTAAGTCATGATAAAATATTACNTTAANNNTNACAATC

ACCCANTNANTANITSFTTTATGNANACACNGCNAGACTTNTTNNTGTTAAT

CATTACANGTCGTGCNATNAAAAGTTAAATCCNTAAANTCGCTANAAATT

NAAAAACACATTTACATACATNACTAAANATCANCGTTTNCAAAATTNAT

TCNATNATATACGNGTCGTNTANTTCTAGACTCCATCTTCTGTATATTCA

ACTACATCATTCNAANATATAACNAAACATTACNATNNTANCGATCCGTN

AAACTNATTTATCACAGTCNNCTGAATNNTNAGTGAATCCTCTCCCTACT

ATACTATCAGCTGNGANANANANACTNACGATAATTACCANCATTACATA

GACCTNNNCCTNTAATACAGGTTTTTANATNNCTCTACATAAATACTATC

TTACACNCTAΓΓCCAATTANNTCNTTCNTTATTNNNAATCATGTCTNTTN

TATCANTACNTAACTTACACTCTANTTCCAAATCAATANCNTCTTTTATT

TCCNTNACTATCTCAANCTCANTAATAAACCTATNNCCTATNCNANCCTA

CTNNCANTCTTTTTATTCNTTTCAACCATCCTTATACACTTCTACNANCT

TTANCNCCCTCTNTTNNCCCNCNNACCCATACNANCTATCCACANTAACA

CAATTCAAANANTTNNCATACNTATTATCACTACTCTCTAANCTTNANAN

ACAATCAACANATNTNATATGANNCTAATCTCACTCATNTCANTACTTAN

TTCCTTNANATTCACTAAATNCNNCTNCNCCNTNCNTCTACNTTCNTATA

CNCCNTTCTCNACTATCAAACTACTNCATATTACCCAATCCTTTACATCT

ATATCCCNCNAANCCCNTCTCNCNCACACNATTACTANNCACNNNNCCTT TCCGCNTACCNACCCCTAATCNATTCCTATTCACCACATCAACTTCAACN

CTTNACACATAACGTCCCNTTCTACTNTANCCCCACATANGCACATTACC

TTAAACCCACACTNACCANCCCCNACATACCCNCCAACACACCAAACCTA

CATACTTCAAATAATTATAGATAATACCCATNAATAATAGTTAATCAAAA

GNTACAACTCAAAGCNATCATTCACTCACGATANAACAAACNTANANAAC

ATCATCAACATNTCAAACACGTAATAANNCCNCAATTCCNANNCNNANCA

NNCCTTTTNTTTTTTCTCTTTNTTTTTCTTACNTCTTGATCATATCAATA

TAACGTAGCTAGGTANATAGCACGGATAACGACCTTAACTCACATATTCT

TANTGACGAANTCCNCGCTACTCATAATTAGAACCTTTAAAGNAGTNCNC

TAGC

>E:\AnalyzedData/50425_2_2_Q l_Kubota_Hp 14_01Run0 l_Cp312_MD l/Q2_Kubota_Hp 14

_01_K14_219.F.esd 1471 MegaBACE

GGGGCCCAAGCAGTGGGTAACAACGGCAGAGTTACGCCCATCGCGNGTCG

GGCCGCGGCGANGGACAGGTGTCACCGAAAAGCGTATACATAGCTTAAGC

GTGGTTCGAATCACGCACTAGTACCGTATAATGACTAGTCTAACTATTCA

AGCGCAGGTACCTATCAACCCAGAGAATGCCTTTTAACACATCACAGAGC

CNAATCTCACATTNTANCTCCGAANTNTGTGAACCANGCAAATCATAATN

GGGAACATACATGGCATGATAGATGAGTTGGATCGCATCTCTCGCTCTAC

ACAGAGGTGTTCAACAAGCTATCTATACATCAACGATATGGTAAAATGAC

TAAAGATTAGNTGGCAAGAGGAANTGTAAGATATCCCCTTAAAAGAAAGA

GTAGCGACATGTTTGCAATATAGAAAAAAATGCNAGAATGCACNGAATAG

AAAAGAATAAGATAAGGATTCCATAGATTTCATATCGATATCTAGTGATT

GTATTGAACCTATGCGTCGTGGATTCANATTCTAATTGACTAGTCCCAAA

CGTAGGAAGTGTTGCACGAGTGACGAAATCCTGTAGTAATATTCCACTAA

TGATTCATTTAGTTATAGGCAGTGAAATGCAACATCCAGGGTTCCAATAA

TCATGCGCTCTCGANATCGCCATTTCAGCGAGGCTTTGTCGTAACATGGA

TGCAGCTTAGGAATCACGNTCCTAGGATATGGAACACACTACTTAAATGT

GCACCCNTTATTTTTACAGAAGAGAGTAAATTTCNAGCATCACCCCTAGG

NAATTAAGTNTTTGGGGACGANCAGGCTTAAGACACCTGTGGGTCTTAAG

GTTACGATATAACGTTGAAATCTATCCCTCTACCAATTTGACCCCTTAAC

AGGACTCTGANAGGGACATTCTGTAGTAGGGTCTCGCGAAATNCATGTGA

ATGGGCCCTCTTTATTACTCCATAGAATNGACGGTGTACAGACTCACAAC

ATGGGTCCACATTTTCACANCTACGAGATATATCACCCTATACACGCAGT

TACCTCGTGCCGCAGAACCCACGCTGGGCGTATCTTACTAGTGCGGGCTT

GAGGCGTACTGATGGNTACCCCAGTTTTTGTCCCCTTTTNTGCAGGGGGN

TAACTTTGGGCGCTGGGGTNACACTGGGCTAACTGTTCCCGTGTGGCGAG

GTTGGTTTCTCCGGGTCCACCCAGTTCCCCCGCGGGGTTACCGAGGCCGG

GNGNCTTTTTGGTTAAACGCGGGGGGGCCTACCTANCTCNCGCCTNCACA

CACAGCCTCGTTTGGGTTGGGGCACCTGCGGGTTTCCCCCGGAAGAAGTG

TCTGCCGCGGGTATATAATNAGTCGCGCCGCGCNGCGGTTANAGTATGGG

CCCGCCCCCCAANACACTTCTNCTCGGGGAGGGGCCACCCCGNGGAGGCC

ANTTCGCAGNTAGTAGACCAG

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_Cp312_MDl/Q2_Kubota_Hpl4

_01_K24_379.F.esd 1649 MegaBACE

GAATGGAGCTCAAAAGCAGTGGTACACGCAGAGTACGCCCAGCGTTGGTC

GCGGCCGAGGTACTTTTTACTGACGCTTTTTGGGTCCATTCCCAGGGGAA

ACCATTTTTGCCTGAACGTTTCCAATTTACCCCGGCGGGTTNCAGGGCCT

NGCATTCTTTTTTTATGGAACTTCTTGGCTNCCTTCCAATCCGCCTTTCC

AGGAATGAAAATTGGCAAGGTTAGGGTAANAAGAATTTCTTTAATTCTTA ACTTCCCGAAGGGAAACAAGGGCGAAATTGGTTCCTCCCAAGCCTTTTTC

CGCCCAATTTTTCTCCGGGCTTTTCTTCCTATTTATTGGAGACTTCTNAC

TTAACCTTGGCAAGTGGTTACCTTTTGCCTTCCCCANGAAGGGCCCCTTA

ATTGGTTCCTTCCGGGGGTTCCAGGCCCCGGAAGGCCCCAAGGTTTTCGG

CGTTTTGGCCGGTTCCTTGGGCTTTGGAAATTGGCCGGGATTTTGGCCGA

TTGGGCCNACTTGGAAGAGCCATTTCCGGTTTCCCCAACCAATTATTCCG

GAAGGGGCCCCCGGCTTCCGGGGTTAACACCCTTGGGCCCCCCTTGGGTG

GNCTGTGTGNCCCCGGGCTTCCGGAAGGGGGGGCCGGTTTAACTTTTCCT

TGGTTCCGGTTCCTTTCGGAAAGGCCGGTTAACCTTGGGATTTGGCGGTT

AANCCCCCATGGCTTTTTTTNGGTTTACCCCCTTTTTTTAACGTTTGGCA

GGGGCTTCTCACATTTTTNGNCGCNCGGGCCTTTTATGGGGCCGGGTTTA

AAATTTCCCAATTTTGGGCGGTCCCCAATTTAAAAAGGCCCTCTTAGAGG

TTATTTTNCCCCCTTTATAGGTGTTNGGNGTCTTGCGCGGAAAAAACCAA

CAGTCTTCTTGGGGTGTTTTAAATTTACCACCCCGGCGGCTGTTNCCAAA

AACGCGATAAATNTTTTCGCCCCAAAACGCGAAATCGAGAAGAGGCCAAT

TTAAAAACCGGGAAAAGGGCCCCCCCNGTGTGGGAAAAGGNCCCNATTAA

ACAAAGACAGGGGTTTGGGGCTTAAAAAAAAATAGGGGCCCCNTTTAGGG

AGNTGCGTTTTATTGGGCCCCCTTTTTAAAAACTTTCTGGGCAAAGGGTT

TCGGGAAAAAGGGCCTTTTAAAAANAACGTTGTCCCCAGAAGGCCATATT

NTTGAAAAACTGTTTTTTGGGCGCCGTGGTTGTTTAAAGGGGCCGGAGNC

CTTATTACCCAACAAGTGCTTTAGGGGGCGAACCCCCCCCCCTAGGGAGG

GTGTTGATTAATAGTCCCCCCCCCNCAAAGGGGTTTTTTNNCCCCCCNGA

TGGTGGGGGGTGGGACACAAAAAANAAATNCCGCGCCCGCCTTTTTTNTT

GGGNGGGCGTTTATTCCCCACACCGGNGAGTTTTTAGATAGTGGACGGCC

CCCCCCCATAANAAGTAGTGAGGGTGCCCACCATTTTATNGTNGGTGGGT

CGGCCCCAAANATAGTTGTTTTTTTTTACNGNGCAAGAANGNGCGAANCT

TTTGTTTGTTNGGGGTCGGCGACGAAAACAANCAAGTTTTTTTTTTANCC

CNCGGACGGCGTGTGTGTGNAGGAACCTCTTCCCCCTCNTAAAATAAAC

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_Cp312_MDl/Q2_Kubota_Hpl4

_01_M02_029.F.esd 1273 MegaBACE

GAGAGCCAAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAG

GCAGGTACCATGATTTAGAGTTTCTATTTTTATTGATAAGGTAAATATAT

TTAGCTTTATGCACTTTTATTCATGTATATCAGAAAAAGCATCTTGGCAG

TGCTTTTCTCTTAAAATTCATCTGATGGGGAAAATGTCATCATTATGTGA

ATGAGGGGCACATTTTTGTATAAGTTAAGTTGGAGTTTACTTAATATTTA

TATAATTAGAGTTACAGTCATTGATTTCATGTGAAGTTTTTGAATACTTT

CTTGTGGAAGAATAGGCAAAGCATAAGAACATTTTGATAATTTCTTAAAT

AAAGGAAAAGCAAAGTAAAAAAAAAAAAAAAAAAAAAAAAGTTCCCCCGG

CCGGAACCAGCGCCGGGGCGCTTACACTCCGGTCCGCTCTGGAGACGCTT

ACTCGGATTGTTACACCCAGAGCNTTTGGTCCCTTTTAATGGAAGGTTAA

TTGGGCCGCCTGGCCTTAATCCATNGGTCCATTAANCGGTTCCTGGTGGT

TGAACATTGGTATNTCGGTCCACACAATTCCCACACACACAATTACACGG

AANCNGGGAGACCATTAAGTGGTAAGGCCTGGGGTNGCCTTAATGGAGAT

TGAANCTTAACTCCNCATTATTGGCTTGGGNCNCACATGGCCGGTTTCCC

AGTCCGGGAAACCCTGGTGTGGCCCNGGTTGGCTTCATTGGATTCCNGGC

CAGCCGCCCGGGGAAGAAAGCGCGGGGANAGGGCGTTATATGGGGGCGNC

ACTACCCCGGGTTTCCCTCCGGTCCAATTGAATTTCCGGGTTGAGCGCNT

CCAGNNCCGTGTCCCGGAGTCTGGGCGGCNAAACCGGNAATCAACGTNCN

ATCTCAAAGGGCGGGGAATACACGGTGTATCCCACACNGAATCAGGGGGG ANAACCGCCGGGGAAAAACCTTTTTGGCCAAAAGGCGCAAAAGGGCCCGG

AACCGNAAAAAGGCCGGTTGGGCGGAGTTCCCCTAAGGGCCCAGCGCCCC

CCTNAGCGAAGNACTGCAACATAGCGTCCCANTAAAGGGGGGGAACCCGC

AGGATTAAAAAACACNGGTGCCCCGAGAAACACTGGNGGCCTGTGATGCA

ACGCGTACGAGAATGACGTCNCAGAGAGGGCGNGGACCAAGGCAAACGGG

TAGGAACAGGNGTGACAGCCGGCAATATCCAATANCANNTACGACCGTNN

CANTCCTCATCGNGCGCGNCCTG

>E:\AnalyzedData/50425_2_2_Q l_Kubota_Hp 14_01 RunO l_Cp312_MD 1 /Q2_Kubota_Hpl 4

_01_M04_061.F.esd 934 MegaBACE

GATGGAGCTCAAAGCAGTGGTAACAACGGCAGNATGATACGCCCATGCGC

GAGTGCGGCCGCGGGACGAGGGTGACTACGGTCTACGCGGAGAGAATTTC

TCGTGTGGNGCTTGTTGCTNGCTTCTCAATTAGTAGATCTCTGTCCGANC

CGTTCAANATTGTNTANCACCATGAAACTTTGAATCCATATTTNCGGAAT

TGAGCAGATNCTCAGTCGAACCGTTCGTTCGAATNTAGTTCAAGCATCGG

AATATCCCGCTAGTGATGGTTCTATATAATGAAGCGACCATGAGTGCTAG

ACACATGACTGCAAACATGCCTGACCACAAAATACATCCTCCATCACCTC

CCATATGTCATNTACATCAATGTCGTAGTTGATGACGTGTGAGCAATTTA

CTTNGTGGTCGGGCTAGTAAGCGATCGATAGCGCGCCATNTANTGTTGCT

CTCGATATTAGCCCAACAATCTGGNGGGGATATCCCTATCAGTCCCACTT

CAATTCCACAGCNGACGTCTGCATGAGAGACATGTGCTATCTGCGAANTA

TGTGCTTCAGATAGCTTAGGGGTCTCCCNGTTGTCAGCNCGTNAGCAACA

CGAGCTTAACTACTGGAAGACTGACAACGATCGCCCCNGGAAAAATACTT

CCGCCCGAACGTTNACCCTTCGGTACCGCTTTGTGNTGACGAGGACCGGA

ACTTTCCGTAAGTGTGCNGGTTAACCNTCCTTGAGCGGTTTATGGTTTAA

CCCCAAGCGTTGGCCTTTTTTGGGGAGNCTCCCAAAATTTCGGCCCCCTA

ATATGGTTGAGGTTCCCGGTACTTTAACCGGACGGCCGCACTTCCACCTT

TGCGNGCCGGCAGCGNAGTNCAGAAACGAGACAAAACAGANTTCCGAATG

AACGCTTAGGAGAACAACGAGCACCTAGGAGACG

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_Cp312_MDl/Q2_Kubota_Hpl4

_01_O02_031.F.esd 1046 MegaBACE

GATGAGCTCAAGCAGTGGTACACGCAGAGTACGCCCXXXXXXXXXXXXXX xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXGGCCTTGGTAACCAGTTCCTT

NCGGACAAGAGTGTGTCTGATTCGNAACAACCCGTGACGGGGTTTNTGNC

TCTCNCTCNCTTCCTCCGACAGGNCTNAGACTGCTTTCAGGGTGCGCGGG CCACACTTGGTGGGNATAAGGTGCCCTGGGAGGGTACCCTTNANGGGCGG

GCCGTTNTCTCTCCCTCTCNTCCCCTCTTGTCNNCTTCCTCTTCNC

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_Cp312_MDl/Q2_Kubota_Hpl4

_01_O08_127.F.esd 1913 MegaBACE

GAATGGAGGCGCCAGAGACAGGTGGTTAACAATCGCCAGTAGTACGCCCG

TGANATCACAGAGCTTCACTTATATCGTGGTNCTCGAGTGTCGTGCACGT

CCCGAGCGTCTAGGCGGTAACGACACAATGTCNGCTATGTCGTCCTGGGC

ATATGTCCCTATTCACAGAAACGAATGCTTGATGACGNATAGTTCCAGCA

AATTGCGNAGACTCGTGCACTCCGCTGTAGTGTAGTCTCAGTATGACATA

GATAAGCACTGCACTGATGGTGGCATGTTCAGTGAGAGACGGAGAACTTG

CGCAGAAATGACCAATCCGCACTTGATCTCCTATTGAAAGTTACTNATGC

TGGTTGTCCCCCTTCTTGTCGAAAATCGAGTGTCGCGAAACCGGGAACGA

CACCGAGCGGAATATCACCTTTTCNCACCCCATATCACTATGGATTCCNT

TCTTATTGGAAGCCATCGGCAAATCAGTATCATGGCCTACCATTAGATNA

CTCGATCNGATCNGTATCTGGATGCTCTCTTTCAGTTACTCCTTCAANTC

AAATCTTCCCNGTGCACCTACTGTGTGTACACACCACATACAGGGACTGN

GTAACTATGGATGTACGNAGANNGAAAACGAAATATANCTCCTCAATNGT

ATCCGTACTTACTTATTACCTATTATGGTAGCCTCATCTCGTTTCCTCAC

CTACTTGGTATCATTCCTATCCTCAGCTNTCTCATTTGGTAANATGNGGT

ACTCACTCTTAGTACNGGGGTGTGCCTTATTTCATCGGCCNATCCTTACC

ACTATACCGTTNGNACCGTTTTCCTTTCGTGGTCCNACCTCTGNTTNCTC

CCNTACTGGCGNCATGCATGTTNTGCTGCCCTAGCCCGGGTCACGTCGTA

GCATAAAACCTATCTTCGTTTTGTAGGGTCCNCCTGGTCGGGGGAATTGT

GACCNTTCGTTTTGTTCAATTCTGCTCGGGTTCCCCCGATCCATTTGGTC

CGACTTCTTTGGCCAGNGATCTAGNGTCCTTGGCGCCCACCCTGTGGGGN

TCCACCNTTCCACTTATCGNCCTTTGACCTCCACTNGGTTNCGNCTTTCG

NTCGAAGCAGTGGCGGTCTCACCGAGGGTCGNTCNTTCCGTCTGGTTCTC

TTGGAAACNTTCGGGTCCCTTTGGAGTCCAATTAGGTTGGTCTNTTATAC

TTCCTTTTCCTCCTTTGACCCCTCTGGGGTGGGGGCCTTGCCCTCCGNAA

GGACCCCCNTAATTATTNTTNGGAGGTTTCCGGTTTCTCCTCNAAACAGN

GGGGNCGGTACCTCCTGGGGCGCGCATTTTGGGGTTTTACCCCACCCTCT

GGCGCCTTTTTNGGGAAGGCCTNCNCCACACTTTTCCGGGGCCCCNGTAT

TAACTCAGAGCCGCGGTTCTAATAATAGCGGCTCGGTCCACACCTAGGGG

CCAGNCGCGCGCGGGTGTTTACCACCCCACTCGTCTGTCGCNCGGTACAT

NGGGGGNCACCACCCCCTGGGGCTCCCCCCGTGTTTTTTTCCCCTCTTTG

ATGGGGAGGTTCACCCTCTCGTTCGCCCCAGGTGNGGGCTTTNTGGGGGC

CANAGAGGGCGCTTCGGGGTTGGGATCTCCTAGGTGGTGGCCCCCTGGGT

GGTCTGGTGNNGGTCGGCGCCCGCGGTTGGTGGGTCGATTATCAACCGGG

GGGCGTNGTTGANGATGATAGACCCACTAATAATAGGTTTGTGGCCGGGC

GGGCGGCGTCCTTTGTAATGGGTCATNNTTTCNTTAAGATACCCACCGGC

GGAAGAGGGATTCGAGGTCGTATTATTGGCCACCCCAACTAGAGAGGTGG

GCACACACCGAGCGTGGGTTTNACCANTCTTCAACAATTATNNACTNCTC

CCTTCTNNCANNN

>E:\AnalyzedData/50425_2_2_Q l_Kubota_Hp 14_0 lRunO 1 _Cp312_MD 1 /Q2_Kubota_Hp 14

_01_O24_383.F.esd 1086 MegaBACE

GAGGAGCCAAAGCAGTGGTACACGCAGAGTACGCCCTCGAAGCGGCGCAG

GCAGGTCNCTTAACCATTGTATAGGTTAGAAAAAATTNCAAGGGTTTTGG

GTAANTTACCAAACAGTAAATTGGCNCAGTTTCTTANTCTTCCAATGGCT

NTCCTAATTAAACACATTATTTTTTTAACTTCTTAATGGCTAATAAACAC GGGTTCAATCGGAGGTNCTTAATTAACTGGCTTAACCAACAAAGCAGTNC

TNTATGAAATAATAANTAACCTTAATAATANCGGTAAAATATACTTGGCA

CAACGGCAACATAGGTGGTAGTGGACGGTGGATTGGTCCTTTAATATTAA

TAAGTAACAGCTTTTCCATTGGCTGGCTTTGANTTTAAAATCACTGGTGG

GTTCTTGTAATGTAACCTCGGCCGGCGGAACCACGGGGCGGTTACTTCTT

GGTCGGTCTTGAGCGGTNACTGGATGGTACCCAGCTTTTGGTGCCCTAAT

AGTAGAGNGCGTTATTAGCGGCXXXXXXXXXXXXXXXXXXXXXXXXXXXX xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXAGTTAGCTAGGACGTATCCCATAAGGTCCGGCCCCCT

GAAGAGAACATTCAACAAATTCAGGCGNCACAGTACAAAGTGGGAACGTT

TGGTCTTCTCTNATNANCGGGTTTCCCCGATGAAAA

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_Cp312_MDl/Q3_Kubota_Hpl4

_01_B01_002.F.esd 1870 MegaBACE

GATGGAGCCAAAGCAGTGGTACAACGCAGTAGATACGCCCTCGAGCGGCC

GCCAGGGCCAGGTACTGCGTGTGGAACATGATGAGTAGCCTATTTCGGAG

ACCGAGCATTGAGAGTTGAATCACTCTCTCNACAGTTTAACTAGGCCGAT

TATTGTGACTGCCCTNGTCTGGTTGTGACAAGATCAGCCTCAACATTATA

CTCACCTAGAGCATCCNTGCAGGGAAGAATACATTATCTATCCTCGTGTG

CTAAGTATCTCATACCTAGCATTTTAACTAGTATGCGTTCAGCNAAACTT

TCTAGGACNACATATATATTTTAAGTCNATNTAGACNCGATACACTAAGN

AAAGCCAGGCCAATAAATCGGGTCGTCGCAAGACGACAAGAAACCCCCCG

GTACCATCGTTGAGCCCTCCGGTGTCTCNGAGCGTACCTCNACCCGCTCT

TTGGGGGTCGGGTAAACCTCATCCTTGGTTACCGGATACCTCTCGTAGGA

CGCTTAGCATGAAGTTGGGGTTCACTCACTATGNTCTTGTGTATGGATGT

GCACCCCTTTTTTGGGGTAGTAGGAGGCTNTCTACTATGNNCAGCNGTCT

ATGCGTGNGGTCCAATTTACNCGGTTGCGNTTCTCTCCACGTTTTGCTTT

GGGCTCGTGCTTGGTTGAANCAAGGTTGGNCTTCGGTTCCCTGGCCTNTC

CCACTGGGGGTATCCCGGCCNACAAGGGCAGTTAANGCACGNGCANNTNT

CCCTGGNGGCGTAGGGNCGNTTTAAACCCTTGNNGGNTCACCCGGGGGGC

GGCATTCGGGAGCGGGCTTGGNCGNGACCAGTTTAGCANGTTTCNNTTAG

GGCCTNAGTCCTTCNCGAGACACAGITCGGTTTATTTTTGGATTTTTTTG

TGTNCACGCCCNCNCAGAGCTTTGGGTCCCCCGGGGTCTCCCCNTCCAAA

GGANGTCCGGGGAAGGGAGAGGGTATCCCCTTTGGTTGGGTTGGNCGCGC

CCTTATTGGTTGCGGCGGGTTTNTATNTACCTTGNGGAGGGTTTCCCGCT

GGCACCGCCATCGGGTNCCTGCCCCGCGCTTGGGGTAAAGGTTAAGCACG

CGCGTTGTGTGTTGTGGCGTNNNTNGGTTTTGCGGGAGNCGGCGTTTTCC

CTTTATCCCAAGGGATTTCCTCTTTTCCCCGGNATTCCACCGGTTGGGAA

AAGTCTCCNNTAACAGGGGTCCGTTCCNGGGGGCCCNCTTTCNCGGGGGT

TNCCCCCTTGTAGGATTTAANCGTTTGGTTNTCTTAAGGTTTCCCAAACT

CTTTNTAAAAGTCGGNGGCGGGNTTTGAGTNGCCCTTCGTGTTNGTCCCC

CAGCGCGTTATATCCCAGGTGNNCGGATTAAAACCCGCCCTNGGTCACGT

TGGCCCCCCGTTGTGTGCGCATTCCAATTGTGGAAGGTTCCCGGGCCCAA AAACGGGCCCCCGGGGGCACACCACGTGACGGNAGTTTGCCGNTTTGTTT

GGGGCGGCGGGCCGCTTTTTTTCCGCGTTACTGTCGTCTCCGNCCNTGAC

CTTTGNGTGGGGCTTTCGGGACGAGTTTCGGCGGTGTCCGAGGGTCGGGG

NTTTTGGCGGACACCCCCGACGGGGCTGATAA AAGGGGTCCACCGNGGGG

GTCCCCCGGGACCTCTGCCGCNGTTNGTCTCCTTCTTTCGTCTCTCCGCG

TGCGATAGGCGCGGCAAACGCTNAACGTGCGTCCTTCTGGAGGAGGTCGG

TTTCCTACCACCGAGCNGTTTTCTAGAGGCTCGTTGCTTGNCAACCCGAG

CGGTTGTAAACCCGCCCCGAGACGTCTGTCTGTNTGTTGTGCGGATCGCG

CGACTCCGCGGGCCAACCAG

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_Cp312_MDl/Q3_Kubota_Hpl4

_01_Bl l_162.F.esd 1916 MegaBACE_o

GATGGAGCTCAAAGCAGTGGTAAACAACGGCAGAGTAACGGCCCATGCGC

GATGCGGGTCCGNCGGCAGCGAGCGGTGANTACACGAGCGNCTGTTGTCN

GAATTGGTCNATAATANCCACTAGAGACTAGTCATACAAAAATGATATAC

ATAACAACACCATGATCCCACTATNTAAAGAGATNTAAACGAGTATANGT

GCACTACTTGCCTACCACATAATGTAAGNGCTGAAGTGAAACGTGCAATA

GCGTTGTGATTGTACAGTTGCGATTTGTGTCTGTGGGAAGCAATGATACA

GATACGAACACTATTGTCCACTTTTCAACCACTATCCATATATATACGTA

AACGGAAGTAGACTAAGTGGATGTAGGATCAGTATCATCAACAGAAGATT

AACGTGTGAGTCTAGCACGTAAGAGTCTNGGATTTGCCATAGGTTATAGT

AAATACGTAAATTTTAGGGTGTTAACACGCTCCTTCGCTCACCCCCTTGT

CTCTCTTGAGACTGGGACTCTGGCATTCTGAAAGTGTGTCGCTTACTTTT

ACCTAGAGTTCTGGGTTCTTATTGTAGGTCGATTAGCGTAGGGAATGGGG

GTAATCCCCAAGGACGGTGTATATAGTTTTGCCCTCCGTTTCTTAGTTTT

GAATGCGAGTTTTATAATTTTGNCTGTCCGTCTTGTAGAGACNAGTTGAC

TTTCAGTTGAGGTACCTNCTAATTTCCGTAGTTTTATTGCCCTCATTGGT

TCGAATGGANAACGACTATGTGNTTTGGCTCCCGGGCCATCGCAATCNAA

TTAATTCCCCCACTNTCGGTATCCAATTACTGCAAGTCCCCCGGGGGGCA

TTGGCCNCGGTTATCATGCCGGTNGGACTATTGTCACCTTNGGGGTGGTG

GGTTTGGCCCCTATATTGGGGAATCGGTTGGACTGGCCTCAATACCCTTT

CCCTACCCCATTTTTGGATTTTTGACCGTTTTGGACTGGNCCTTCCAACC

NTTGACCCCCCAGGCTTTTATTCCCCCAGTNCGGGAGAGGCATNGNCCCT

ATGTTTCGGAGATTGCCCGCAAGACTTTTGTCGNTTCTTGGTTTTGGAAC

CTTCCGGGGGTTCGAAAACATGTCACCGCCGTNGTGTNAACCGCATGCGG

NGGGCCTGTCGGCAAAGTCAAATTGGNGNGGCGGGCCTGCTTTATTCCTC

CGGCGNTTGTCCCGTTCCGTTCTTGCCAAGCCTTGTNACCTTCGGACTTT

GGGTCGCCCCCCGGGTCCCGTTTCCGGGGGCCNCCGCTTGTGGCNGGNGC

CAGGGTGNTTCCCAAGTCTTTGCCAGCTTCCCATATAGGGGGCGGCTTAA

CACTCNACGGNGGTTCCATTCCCCCCTCCGGTGANTCCCTTGGTGGGTGC

GNTCAAACCTTNCCCTGCTGTACCCGGACATTCATTTTGTTTGTTTCCCC

AAATGAGGGCCCATTATCCGAAACGGGGGCGCGGGGGCAACNCCCGGGTG

TGAGTAAAAGGCCCCTCGTTTGTGTGTTNTGCGCCCTGTTTTTNTCTCGT

AAGGGGNCTCTCCGCGCCCCCCTTTTTTGGGGGGGGTGTNCGCCAACATG

GCCCGGGTTTCCAAGTTCCGAGGGTAGGCCGGGACCCGCGNCCGGGGGGT

TTTAAAGAAACCAGGGGGGGTCCCGGGATCGCTCTGTTTNTGTCTTTCTT

CNCTNGTTCTTCCTCTCTTGCCTGGTTCGACAGCAGAAATTGCGTCGGTC

TGCTATNCTTCGGGGAGGAGGGGGTCTACTACTCACCCACCGGGGNGGGT

GTCATGTTCTACTTAGTGCTGNTGCGCCGGTNAGTGTGANCACAACCCCA

CGCCGANGGGCGATCTACGAGTNATGTTTTATGTCGCCNCACACGAGCNC TCGGGCAGGAGGAGAG

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_Cp312_MDl/Q3_Kubota_Hpl4

_01_D17_260.F.esd 1077 MegaBACE

GATGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAG

GCAGGTACACAGATGCCCTCCACAGGGGCTGAACCTCCGTCCCCACAGCA

GTCACAGTCTCCCATGCATGAGGAGGCACCAGAAAAGGTGGAGTCTAATC

CATCCATACAGCCTCAGTCCAGACAACCGGCAGCTTCTGTAACTCCCCCC

CAGCCACAGCCAGAAGCAGAGCCCGCAGAGGAAGATGAAACCACGCCCAA

ACTGCCATCCCCTCCAACGGAGCCCCCCAACAGCATTGATTCTCCTGAGG

AGGAGTTCTTAGAGGCTCAGGCAGGCTCTCCCTCAGAGGATGATGATGTG

GGAGAAGAGGAAGAAGCAGTAAAACCATCTTCTGCAGTCTCACAAGAACC

AGTTCCAGTCGCTCAGTAGTAGACAATTGGCCTCATCAATATTTAGACAA

AATTAGGTTTGGGAGTGAAGATCCAGTTGGTGTGTATAGGGAAAGCCTGT

ATATTTATGCTGTGTTGGATGTTGATTTCTGTTTTGTAGCAAACAAAAAT

TCAGTAAGATTTAGCATAAATAGACCAGGTGTGTGTGTGAATGAGGAAGG

ATAAGATGAAACTGGAAATTTATTCCAAACCATATAGAANTTATATATGA

TGAGTTTATTCTGAAATGTCCTTTATGGAGTGCTTAGAAAGAAAAATGTT

ATTCTATTGAAACAAGTATCTACACTGACGTGATTATTACAGATTTCACG

GGGATTTGTACCTTCGGGCGCGAACACGCCTGGCCGTACTTCTGTCGCCT

NGGACGTTCTGGTTGTCCCCAGCCTTTTGGTNCCTTTAGNGGGGNTAATT

GGCGCCTGGGGAACAGGGCCTAGCGGTNCCGGNTGAATGGGTCCGCATNC

TGCCTCATTCTTCGCCNCCGGNGTCTATGNGTACCGCGGGGGCAAGGAGC

ACCCATTNTAGTGTGGCCAGGCGGTCTGGAATGTNCGCGTTGATTCCCGG

AGAGGGTTGAGCCGCACAAACGGTCGCGGGGGNACTACTGTTAGGGGAGA

GTCTGCGCTCNTNCNCCNTTGCCTTGG

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_Cp312_MDl/Q3_Kubota_Hpl4

_01_H07_104.F.esd 1049 MegaBACE

GATGGAGCCCAAAGCAGTGGTACACGCAGAGTACGCCCGCGTGGTCGCGG

CCGAGGTACGCTATATGGCTCTCTTATTGTGTCATGAATGCACTTGATTG

GTTTGTTGATTACTGATGGTAAGTGACTAATTGGTAATGACAATTATTTA

AAAATTAACAGGTTCGTGGTGACAGTTAATTGAAATGCAACTACGGACAC

ACTTGGAATCGTCCAGTCGTCACAAACCTCTGAAGTGCATCTGGAGCTCA

TTTTAAAAAAAATGGATTATAGACAACTTCACCAGTGTTTATTTAAGGTT

TGTGATTCCAACGTCAGATCTTAAACCTACCAGCTAACTGTGTTTTGTCT

GACCTGTGGCCAAGATTGTTGTCTTTATTAGAGTAGGGAAAGGCTCTTTG

GCCCAAAGTTAATTTGATTTTGTATACTTAAACTAGACATTCAGTTGTGT

GTGTATGGACGTGTGTGTGTGTATATATGTATATATATATATTGAAAGCC

TGATGGCATAGCCATAAAAAAGATAAATTCAGCTGGGAATTTAATACATT

CTGTAACACGATTCCCCCAAAAGTATGTTCTTATTGCAGGTTATCCTTAT

AAAGAAGATGAGTAAATATTTATGTTGCTAAGACATACGGAGATGATTTA

AGAATTTGGAAATATGTTATATTATTATTATCAGTTTTATAATTGTCATC

ATCATTATTCATTATTGTGTGCACCATCAGGTCCGTTGAAAGAAATACCG

GACGCGAACAGACGGTTTTGCCACCCCCTTGGATGACGTTGAATTGGTTT

TGNTGGTCCACAGCGTTCCTTAACATCTCCTGGAATTCTTTACTGGTTAC

CACCAATGATTCATATGCTGTCCCTGGTAATATTTACAGGGCTTTTGCCT

CNNCTNGNCCNCCNTNNGCTGCTCGCTCNTTTCTTTGCGANCGTTGTCGC

AACATGGGGAGAACAATCAATAGGTTCTCTCTGAGANTGTGTCGCGCAAA

AAAAACGCGCGGGGGGAATTTACAAGAGCGCGACCTCATGAGGCGCNCT >E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_Cp312_MDl/Q3_Kubota_Hpl4

_01_J07_106.F.esd 1426 MegaBACE

AGTGGCGCCCAAAGCCGTGGTACCAAGCGGCAGAGGCTATCGCCCAGGCG

CGGTCTGCTGGACGACGGTACGTGTGCCGAGGCGGCCTTTTTTTTTTTAC

TAAAGTTTTAGTTATCATATTTCGACAAAAAAACAACTATGCATATAACA

CTGTGACATGATACAAGAGTTGCCTTACGCTATCGACTTGTATGTGTATG

TCTATATTAGAGCTGCANAACAAGGCTATTCTTCAAGACATCTAAGGAGA

TCTACCTAAATACNTATAATNATAGTATAGTAATAGNAGGGGGTGTACTG

GACTACCTTAATCTCAGTGGACACAATAGAACCAGTAGTGTAGAATAAGT

AGACCAGAAATATGAAGTAACAAGNATTAGAATCCCNTAGCATAATCATT

TACATTGCAAGTAGGTGAGCTCCATAGAACNACGTCGAACNGCTTTGAGT

TGAGTCGCATACGTTAACATGCCCTTGGCGGCCGCAGTCGAGGGCGTACT

TCTGCTCGTCTTGAGCGTACTGACTGGTCNCCCAGGCTTTTGTNCCCTTC

TCAGGTGGAGGGTGTTAGATTGCGCGGCGTGTGAGCGTACTCGCATGGGT

CTATAGCCGTGGTCTTCCTGTGTGGAACAGTTGTTTATCCGCTCAGCATA

TTCGCGACNAGCTAACATTATCGGAGCCTCGGTGGAGTCNTTNGACGACG

TGTGAGTAGCCTGGGGGGTGCCTCAAATGGAGGTGACGCTCAAAGCGTGC

ACTATTTAACTTTGGTCGTTTGTAGCCGTCCTCCAGCGTGGCCTCGCTTT

TTCCCTAGGTNCCGGCGAAACACCCGTGGATACTGCTGGCCCAAGGCTTG

GNATTTCTAACTCGAAGAGTTCAGGTCCCAAACGCCGCCGGGGGGCAGCG

AGGGCGGGATATGGCCGTTCATTGGGGCGCCTCCTTTCCCGGGTTTCCCT

CGGCTTCATTGGACTCCGCGTTGNGGCTCGCGNTCGTTTCGGTTGCCTGC

TGAANGCGGGGGAATTCAGGCTTNCCAACTTTCCACAAAGGGNCNGGGTA

AGTACCGGGTTCGTTCCCCCCCGAAATGCGCNGGTTATNGCACGGAAGAC

CTTNTTCNCGCCATACGNGGCCCGGTCTAGGGGCCCTGGACACCGTCAAG

AAGGCCGGTTGGCCGCCGGTGTCCATGAGGGTCCGCCCCTTTAGAGATTG

ACTCTCCGNCNACNCTTCTATCGTGTGNGTGCGCTCCCGGGGGNATAATA

GGTACCNGGGTGCCCGGGNANCCACTCGGGTCATGCTCANCNACCGGAGT

GAGTGATTGCCGTCCCTCTCGGAGAGGGGTCTTCTACACCAAGGTGTNTC

CTTGGGGNCANGAGTCNTCGCAGCCCGGCCTNTTTGTTCGCTACTTCGNC

CGTCTTNCTCTCCNTGTCGNNGNCCG

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_Cp312_MDl/Q3_Kubota_Hpl4

_01_L15_236.F.esd 808 MegaBACE

GATGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAG

GCAGGTACGCGGGGAGGAGCAAAACAAGAAAAACGATCGAGTGACCCAGA

GAGAGCGCAAGAGCATTTTCTCGTCTTTTTACATATATAAATCAAATATT

TGCAAACATGTCGAAGGTTTTCCTGGCCACCAGTCGTGCGATAGCGCCTA

GAATCCACACGATGAGGCAAATTCATATGACAAAAGTCTTCAAGGGGACT

TTTACATCGACCGATGCTGTTATGCCAAAACCTCACACGACAAAACTTCT

GGGATATATGGGAGTGACTCTAGCGACTTCTCTGGGAATCTTCACTGGTG

CTGAAATAAGCAAGAATATTGCAAGCTTCCTAGAAGAAAATGAACTATTT

GTTCCCTCTGACGATGATGATGATGATTGAACATCTAGGTTTTCACAAAA

AATAGAAGGTTTGTGAATATGCATCTAGTCTCCTCCCCTCAACGACTCGG

GTTTGGACTCCAAGAATCATGTCCAGTGCATGAACCAAGACCCTTATCAG

TAGTTTTTAAGAGATGTTTACCAATGTTAGAACTGCAGATATTATATTTA

AGTAATTTGTTGTTGAATTAAAGTTTCAATATGATTATATGAACCTGCCA

ATGTATCATCCATAGACTTrTGCCATTTTATTTACTTATTTTAACTTTTT

TAAAAGGGTTGGACTTTTTCTTTTATCACTGGACACACTTGGGAGGGCTG

TCCAGTTCCTCCTTGTTTTTTAAAAGAAACTTTACCATTTTGGATCCACC AGGTTAAG

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_Cp312_MDl/Q3_Kubota_Hpl4

_01_P01_016.F.esd 435 MegaBACE

CAGCTTAAAANNGGTANGACGNATGCAGGCATCCCCCGTGTGGNTNTTTT

CCTCATGGTGTGAAGATTGCANGTGGGACGGGACATCAGATACCACATGG

GCAGCCCGCCCNGCGAGGATAGACGNTGTGAATATAGGCGCAAACGAGAG

AGTGGACTCAGTGAATCGCAAANCAGCNNCAGCNTGCACGACGNCATCAA

GTAGCACGGANTAACGCCTCACANCAGCCAGGTCGACGAAGCGCAGACTN

AGNNAGAGGAGAACNGCNAACCANNGTAGCATAGACTACCTGCCTCANAC

ACCATCGNGNTTAGCAACNCAGTATCCANGAAANCNGATACCGNTCGAAC

CCCGAGTCTCCNTGCAAGGATGNTCGTAAGCATAAAAGAACAACGATCGC

GCAACCTACAGAGAACANAAAGCANTATGCNCAGG

>E:\AnalyzedData/50425_2_2_Q l_Kubota_Hpl 4_01 RunO l_Cp312_MD l/Q3_Kubota_Hp 14

_01_P21_336.F.esd 816 MegaBACE

GAGGAGCCAAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGTCGCGGCC

GAGGTACGCGGGAAGTCCTGAAATGTTTAGCAACTTTCGTTTTATGGGCA

AATGAAAGTTACTGGTATCTCTTTGATGTATATGAAAGTGAAATTGTCAA

ATTTTACTTTACCATTCCAGAAAAAGAAAAGGAAAATAATTGGAATAGTG

TCTTTATGAAATTAACATCACTAAACAATTTATATATCTATATATATATT

TAGCTGTTTCCATGATGATGTGAACTTGTTTATATATATATATGTATATG

AATATATATGAAAGGCTGTGTTTCTATATTTGTGGCTATATATTTGACAG

GCATTTCATTTTGTTATCACAGCCATCTTTTAACTTTACTGTGGAGCATA

TGTATCAAGCATCAATAGGAAATTTACTTGATATAGGTTGCTTTGTGATT

CGTGTAATTTTAGTGTAAGTCGTCATATTTATTAGTTTAATGTGAACTTG

CGCNAAATTACCTTTTGTCCGGACTTTGTGCAAAGCCAATGCAGTATAGG

AAACAACCAAGACCATCTGTATAGGCTTGAACTTTGACTTTTACTNTTTC

CAAGGTTAGGGGAAGGTNATTNTCCCAAAAACTTTGGAAAAAGCGGCTTT

TAAATTTCCTAAGAAATTTCGACAGGCAGGTTTTTCCAATTATTCTTTGG

AGTTAACCCTTGGCCCTTGGGGCGGGCCGGCTCCGAAGGGGCCGTTACTT

TCTGTACGGTCCTTGAAGCGTTAACTGATGGTACCCAGCTTTTTTGTTCC

CCTTTAGGTTGAGGGG

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_Cp312_MDl/Q4_Kubota_Hpl4

_01_B16_242.F.esd 925 MegaBACE

TGAGCTCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGCC

GAGGTACCCTCTCCAGCAAAAGATTCAAATTCATCAGGCTCTTCTGGTTT

ATTTTCAGTATTGTTCACTTTCGGTAACTTTCTCCAGAATTTAATAAAAC

CTATTTCATAATCATAATCTGGAATCCCACGCTGACGGGGCAATTTTTTA

AGAAGTTCTTCTTGAGGAAGTTGTAAATTCTTCTTTTTCCCATCTAGTCT

GTTTCCTGAGCCTTGGAAAGAGTAGAAGCCTGTAGGTTCTGGTAGCATCT

CCTCTTCTGTATCCTCTGGCAAGGCTGCTGCACTGTCCATTCTCTCTGGC

TCCTGGTAGCCCACTGGGGCAGCAAACTCCACGTTCATATCACATTCGAT

GATGCTAACAGCACTCCCAGGCTTGACCTCTAGGACTCGCATTTCATATA

TTCTATCATTGTAATTAATGGCAATAACATCGTCAGTTGTTAGACAAGCA

AAGTTTCGAAGCGCATTTTCCAAAACAGCTTTGGGATTTGTGATATCAAG

GAACTCCACATTCTGAGGTTCAAACTTGGAGAACGTAGCAACAGGAAGTG

ACACAGACTCGATGTGAATAAGATCCCCCTCATCCAGCAGCAGATTTCTC

ATCATCCAATAAGGAATATAAACCTTTCCTTCATCAGCTACAAATTCTAA

AAACACCGCAATGTGTATTTCTATTGGCACGTCGGTGGTTAACTTTGAAT

AACCATTGGGTAAAACCAATTGTTTAAGACGTGTCAAAACCATCCAGAAG CTGAANGTGGGCTTCTATAATTTTTGGCCACCATTTTCCAACTTGATTGG

TTTGGCCAGCACCCAGGGAAACCATTGGGGATTNTCCCCGGAAAATAAGA

CATCCGGGAATTTGCGCGCNGATTG

>E:\AnalyzedData/50425_2_2_Ql_Kubota_Hpl4_01Run01_Cp312_MDl/Q4_Kubota_Hpl4

_01_H04_056.F.esd 1312 MegaBACE

GATGAGCTCCAAAGCAGTGGTACAACGCAGAGTACGCCCTCGAGCGGCCG

CCAGGCAGGTACATTTGTCATGCTCCCATGATAAATATTTGCCGACTGTG

TTATTTCGGGAGGCACATCTTGAGAAAATGACCAAGTTAAAAGGTNAGCG

AGATNAATAAATAATTGATAACGAAATAAAATAAGTGAATAATAATTGAT

ATACAACTAACAGATTNTCCTTGATAATATACAAGGTTTAGATAGGATCA

AGCAATATTGCGTAAGCTCATCAGGCTACGGCGTATGTTGAGAACGATGA

CGCATGGGGTACAGTACNGATGAAACGTCGGGAACCCCCCTTTCGCCCAA

GACAAATAAGAGAGAGAATGAAAATGCAGCAAGAAGAGAGAGTGCAGATC

ATTTGACTACAAGTAGCGAGCCCAGCTAACGATGGATAGCAATCTGCTTA

TATCCATCTACCACTCTCTTCTATCTTTAAGTGAAACGTGGGTTGTCGAC

CCCTAGAAGCTTCTTATCCACGGATAGGAGTTATGGTAGGGCGTGGAGCT

GGGGATCTTGGCTCATCAAAGAAAAATCTAGCGTTAATATAAAATAGACT

AAGGAGCGGNGAAGAACCAATGAGGAATGGTATTATACNCTTCTTGCTNN

TTTATGNANTGGTTCAGACGCATATCTGGTTACGGATCTCGACAGATATC

CGGATGAGACACACGAGGATGTCAGCTAGTGTTGCGTGCGCTAAGGAATG

CCCTGGGCAACCNGGGTTTGCCGGCTGGCGGTNGTGATTTNTCCCCCCAA

TNATTTGTTAACGCCCTTGGATTTGGTTGCACCCGCTTGACGTCTTTACA

GCATGTTCAAGGTCGACGGCAGAGATGGCTTAACGAGGAANTCGCAAACT

TNAGGGGAAAACNCGGGGGGGGAGGCACCGNNGCCGTTCTCACCCGGTTT

NGCCTCCGGGCCGGNACCCACGGGGNGTTTCTTGGTCTGCGTCTGAAACG

NGTCTGGGTGGTAAGCCCAGGTTTGTGGTCCCCGTTCAAGAAAGGGGGTA

AAATGGGCGCGCNGGGGAAGGGGCACAANAAGCNGGNTCCGCTCGNGGAA

CANGCGTTCNGNCAAAAACGAANCAGAAAATAGCGGACACAAAATAGAAG

CAGNGGGCGACAAAAGCAACACACAACAAATGGNAGGCACAGACAAGCAA

ACCAGGAAAAGTGGCNTCAAAACAAACACGNAAGGAAGATGTNCAACAAA

CAATCACGAGGGGGCCACGTTCCTCGCTTTCGTCNCTNATCGCCCTCCCC

TCTCTCTCTCCG

>E:\AnalyzedData/50425_2_2_Ql_Kubota_H_P14_01Run01_Cp312_MDl/Q4_Kubota_Hpl4

_01_L22_348.F.esd 1852 MegaBACE

AGGTTGGCCCCGNAACAGGACAGTGAATNCAACAAATCAGTCTAAGTAGA

TACAGTCCCCCAGGAGGGNAGTGTCTCAGCCGAGCGGGACAGGAGATAAC

GCTCGAGCGAACACCAACNGCCGCAACAGACCAAAACTNTCAGCGTCTAC

CTGACCTTCCTTACTGCCTCGCCTCATTTCGTCAAGTCATCGCGCGACTA

AATCTAACGTNATAGTGTATCAATATTATAAGTACGTGCGCAATTCTTAA

TCTCCTGTCATCCNATACGGTGTTGGGGTCGAACCTCGTGAACATTCGCA

TTCCGATCCAATTACATNTATCCATGTATTCAATCGCAAAGTTGCACGAA

ACCCACAGATTAACCCGGAACCACAGGTGTATNCAANCCAAATTAGGTTA

CTATTCCATCCACTCTTCCTTGTANATTCAATCCCAATTCAATACGCAAA

TTCGAATNTCGAATCACTCCCANTCCCATCNACGTAATTCAGCCACAGGG

CCATTTTCAAAGTAAAGGCCTAGGAATTCCCCGGTTTTGGTTTCGGGTTC

GGTTCGGTTTTATTTTTNCCCTCNGCCTGCTTGACTCTTGTCCACATTGT

TGCCCCCGGTTGCGGCCATTCCGTGGGNGTTCGGNTAAGCTTTCTTGGCC

NGGTTGCCTCTTACGCGACCTGGCCTAACTCGNCAGCCTTGCCCNCATTA

TTTCGGGCTCGCTGATCAGTTTTAGTATGATACGGTAACTTTAACGGCTG CCCGTGCCTTCCAGATCTCTTTGTNGGCCACGGGCTGCCTTGTTCTTTGT

AATCTCGAGTTCGGGCTCCCGANTGGCACATCTTTTGGTGGGGTAATACC

ACCACCCTTCTNCTTAGGGTCGGGNTTTTGAGNCCCCAAAAATTCTTGTC

AGGACTCNGTCCCGTTGTGGCCATGACCTACCCAATTTACCNCCTCCCCT

TTATTGTCCTGTCCTAATGTCCCTTGGCNGGTTTAGAAATTAAGGTCCTG

GTATAGGAAGGNGCGCCCTTGCGCAACCCAGGTANTTCTGGCCCCCCTTT

TTCCCACTAAACCGAAGGTTTCGGCGGGGCCAAGGCCCCTTAGAAAGTTT

GGGGTCAGCAAGTGCGTGGGAACGGGCCNTGGCCCCCCTTTGGTTGAGGA

CGGGTGGCCAGCCCATTTTAACATTGCCNGGCCCGGTGTCCGGGGTGTTT

GGGCTTGGTGTTGGCGGGTCCTTACCAGGCTTGGGCCCACGGCCGGGNTT

TGTATNCCCCGGCGTTTACCGAGCTTTTGGCCCCCTGGGNTGCCCCCTCC

CGTTAACGCGAACCGGGCCCCCGACTTTCCATTTTTTGACGGTNTTTTCC

TTATCCCGCCTTTCCCGTTTTTTCATTCAGGNGCGACNGGTTATNGGCGC

GGCTGNGTCGNTTTTGCCGGCCTTGGGTTNCACNGGCCTTCTCTCATTCA

TTCCCGNCGGGGGGGCCTCCTCCCNTTTTAANCGGGGGTCTCCCCNGGAA

CTTTTTACAGTTGGCTCTTTTTANCCGGGGGCAGCCGCGTTCAGTAAACG

CCCTTCAAAATCAACTTTGTGCACTTNTACNGGGGTTCGACATTGGNTTC

CACCGNTTAATTGGGCGGCCCAACTTAACCCTCTTAATTTANAAACCGGA

GGTTTTTTCGGCGCCTTANGGANCCTTTTGGAAATATCGCCCGGTGTCAC

TCTAAAAATGGCCGCCTTTTNTNNCCCCGCTTTTGTCCAAAAAGTCTACA

CCCCCTTTTTTGGGGTTTTTACTCCTATTACTTATCATCCTANNTGCGNT

ATATGCTCTGGGAACACCGCCCAGNGTGAAAAAAAAACAGCGGTGTTCTC

CA

Unigene set from WSSV-infected SSH library:

>HP35_SSH_trimmed.fasta.Contigl

ATGAGCTCAAAGCAGTGGTACAACGCAGTAGTACGCCCTCGAGCGGCCGC

CAGGCAGGTACTTTTTTTTTTTTTTTTTTTTTGGCCAATTCGTAATTCTT

AAAGAAGGTCTTGAGGACGTTTCACTGTAGTTAATGGAAGATTTCCGTCA

GTCATGTGTCGTTCGGATCTAAAATCCTGGCCTAGTTGCCCGATCGAAGC

CTTACGTTCGCCGACTGCTGCAATCGACGTTGCCGGCATGGTCGCAGGAC

TTCGTTTCTTGGTTCCAGAGGAGACCGGAGGGGCATTCCTTCTTTTCGGG

CGTACCTCGGCCGCGACCACGGGGCGTACTTCTGTCGTCTTGAGCGTACT

GATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGAGAGTTCAAATTGNCGTC

AATCCANAGNGGNGAAACCCGGTNTTTGTGTTTTNTGCTTTCCCCTGTGT

ANCCTCTGGGCTCTGTTGCNGCCCTGCTTAGAAAGTGAGCTTCTCGGGGG

GGGCTTAACCCNGTATCACGGNGTG

>HP35_SSH_trimmed.fasta.Contig2

GATGGAGCTCAAGCAGTGGTACACGCATNNGTACGCCCAGCGTGGTCGCG

GCCGAGGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTCACTACCTC CCCGTGCCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTAGATGTG

GTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTT

ACCCGTGATAGTCATTGTAGGCGTTTAACCTACAATCGAGAGCTGATAAG

GCAGACGCCTGCAGGACGCGGCGCCGACCCAACCCGCCCGCGTACCTGCC

CTGGCGGCCGCTCGAGGGCGTACTTCTGTCGTCTTGAGCGTACTGATGGT

ACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXGTTTAGCTGAGAGCGGTT

>HP35_S SHJximmed.fasta. Contig3

GTGAGCTCAAGCAGTGGTACACGCAGAGTTACGCCCAGCGTGGTCGCGGC

CGAGGTACTGTTCATCGTAGTTAGCAGGAAGACTAGAGGTGTAGCCGTAA

GGAGTCATCCAGAGCTGAGAGTAGGAGTGGATCGTGAAGAACACCTTCAT

CTGAGAAGCCAAGGCCAAGACTGAATCCCGGACAGCTTGCGACTCTGCCT

CTGAGAAAGCGGATGGTCCGTGGTAGATGTCAGAGCAAGGGCTGTTGGAG

GTTCCGAGGCCACCGAAGTCAGAGTCGAAATTGCGGTTGGGGTCAGTGCC

GTGGCATCCCAGATCGCCATAGGGCACACGGTTCTTACGCCAGAGACGGT

TGGAATTATGAGTGCCCGCGTACCTGCCCTGGCGGCCGCTCGAGGGCGTA

CTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTAG

TGAGGGTTAATTGCGC

>HP35_SSH_trimmed.fasta.Coπtig4

GATGGAGCCCAAAGCAGTGGTACAACGCAGAGTACGCCCAGCGTGGTCGC

GGCCGAGGTACTAAGATAACTTTAATAAAATTAAGAGATAGAAACCGACC

TGGCTCACGCCGGTCTGAACTCAAATCATGTAAGGATTTAAAGGTCGAAC

AGACCCTCCTTTATAACTGCTGCATTATAAGGAAACCTTAATTCAACATC

GAGGTCGCAACCCTTCCTGTCGATATGGACTCTCAAGGAAGATTACGCTG

TTATCCCTAAAGTAACTTAATCTTATGATCTCTAAAAGAGGATCATTAAT

TTTCCAGAAATTACTGTTATTAAATAACTTAAGAACAGTTACTCACCCGC

GTACCTGCCCTGGCGGCCGCTCGAGGGCGTACTTCTGTCGTCTTGAGCGT

ACTGATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGC

>HP35_SSH_trimmed.fasta.Contig5

TGNGCTCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGCCG

AGGTACTGCGACGCTAGAGGTGAAATTCTTAGACCGTCGCATGACGACCT

ACTGCGAAAGCATCTGCCAAGGATGTTTTCATTGATCAAGAACGAAAGTT

AGAGGTTCGAAGGCGATCAGATACCGCCCTAGTTCTAACCTTAAACGATG

CTGACTAGCGATCCGCCGCAGTTATTCCCATGACCCGGCGGGTAGCTTCC

GGGAAACCAAAGTCTTTGAGTTCCGGGGGAAGTATGGTTGCAAAAAAAAA

AAAAAAAAAAAAAAAAAAAAGTACCTGCCCTGGCGGCCGCTCGAGGGCGT

ACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTA

GTGAGGGTTAATTGCGC

>HP35_SSH_trimmed.fasta.Contig6

TGGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAGGCA GGTACTITTTTTTTTTTTΓTTTITTTTTTTTTGCGAAGCAATCGTTTTAT TATGACATCGATCATCGCACACACGGGACGCCCGTCACTCCAAGGGCTAO

AATAAGAACATATGTTGACCTTGCTTCACTCCTTCCCGCTGCGGGTGTGC

GCGCCTTCGGACACCCGCGTACCTCGGCCGCGACCACGCTGGGCGTACTT

CTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTAGTGA

GGGTTAATTGCGC

>HP35_SSH_trimmed.fasta.Contig7

TGAGCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGCCGA

GGTACAACTCTGTCTCAGTTTCAGGAAATTATGACTTTGACCACAAAGTT

GTTATCTTTGTCGCTATAAATGCTGATGGTAGAGAATCTAAAATTGATAT

CAACATTGCTGATATCAACCCCACGTATCTGCCCTGGCGGCCGCTCGAGG

GCGTACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCC

TTTAGTGAGGGTTAATTTGCGC

>HP35_SSH_trimmed.fasta.Contig8

GATGGAGCTCCAAAGCAGTGGTACACGCATTAATACGCCCAGCGTGGTCG

CGGCCGAGGTACTACTTAACACTGGCCCTCACTGGCAATTAACAGAACAG

GTGAAATGAAATGTTGGAACTTCAGGAA AATCAGTTATTTGGAAAAGAAA

TGCCCTTCTCTGAATTCCATATATCATTGTCTGGGAAAATCTGAACACAA

AAGTTGAATAATTCCCCTATGTAAAAAATAGTATAAACGGCAAAAATAAA

TATGTCTTTCTTTATGGAAATCCATATCTATGTTTAATGATAAATAAATA

TGGAAGTTTAAAATGTATACTGTCTGAATTGTAGTCTTTCAATGCAAAAA

TAAATTAATTACAGTTTATTTCCTTACTTTGAACATTAACACAACAAATA

TTTCACTTTGAAAGTCTGAAAGTTTTGGTTATTTAACCATGAAATAAATA

AAGTCTTGTATAAAATATATTGTGATTTAACGTAAATTTAGAGTGTAGAC

CTTCAAATCAATAATCCATAAAATCTGAAGATGTAATGAATAAATTAATA

ATTAACAAACTTTCTAGTGAAAAGCTTGAACAAAAATATTTCTTGTATTC

ATGCAGCTACATTTGCCAACTTACTGAAAATAGTACCTGCCCTGGCGGCC

GCTCGAGGGCGGTACTTCTGTACGTCTTTGAGCGTACTTGATGGTACCCC

AGGCTTTTTGTTCCCTTTTATGACGGGTTCACTTGCGCGCTTTGGGCGTT

AATCCATTGGTTCACATACGCT

>HP35_S SH_trimmed.fasta.Contig9

GATGGAGCTCAAAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCG

CCAGGCAGGTACAGTTACCGAAGATTCCGTGGACTTGCGCTCAATTGTAG

TTTTTCCACCCTTCAAATTTACCCAGAACTTATCCAATTCGATGGCATTC

CAGCGACCTTCGTCAAATGTAAACTCGATTCCGTTGTTGTCTTTATGAGG

CCAGGCAAAGATACGAACTGTGCCCAATCTCTCAGCACCTCCATTCTCTA

CATCAATCTTAAAAGTGAATTCTTTGTGGTTAAGACGAGGCACATATGTG

CTGATTTCCACATCTGGGATTCCTTCAGCATCATCAACTGCGTTGATAAG

ACTGTATTCGAAATCTTCAAAGTAGGTCTCCAGTTCACCTACAACGGCTA

GCTCTGAGATAGACACGCCAGAGAATTCCAAATCGGCCTTGGTGTATGGG

GGTAGACTGTCCTTGTGTTCCCCGCGTACGCGGGGGGAACGGAGAGAGAA

GGAGAGGAAAAGGAAAAAAAAAGGTGTAGATAAAAACATTTTATCTCCTG

ATGTGTCTTGTGTTTGGAACTAATGATGCTTTTGAAGTGTGTTTGTTTTT

ATACAGAAACAAAGGAAGAAATCTTTAAGAAAACTGAAAAGTCTGCTGCA

CAACACCATGGGTTTGATACCATACCTGCCAAGGGAGAGTGTCCTTAGAA

TCACAATTCTGGGGCATGTTTTCCACGTGTGAAATGGGCTCCGCGCAACG

AGCATCAGATGTCCCAACTTGTACCTTNCGGGCCNGCGAACCACGGCTTG

GGCGTACTTCCTGGTCGCTTGGAGCGTTACTTGGATTTGTACCCAAGGTT

TTGGTTCCCCTTTNTGACGGTTAATTGCCGCCTTGGGGTATCTACTGGGC

ATAGTGTTTCCCTGNGTC >HP35_SSH_trimmed.fasta.ContiglO

GTACGGCTCAAAGACCGGACAGAAAGGTAACGGCCCCCTTCGGACCGGCC

CCCCAAGGGGGCAAAGGGTACGCGGGGATTACGAGGGATACGCCCTGCCC

CACGCCATCCTGCGTCTGGACTTGGCCGCCGCGGACCCTCACCAAGACCT

ACCCTGATGAAGATCCTGACGGAGCGTGGCTACACCTTCACGACCACCGC

CGAGCGAGAAATCGTTCGTGACATCAAGGAGAAACTGTGCTACGTGGCCC

TGGACTTCGAAGAGGAAATGTCCACTGCCTCACAGTCTTCTTCTCTTGAG

AAATCTTACGAACTCCCCGACGGCCAGGTGATCACCATCGGCAACGAGAG

GTTCCGCTGCCCCGAGGCCATGTTCCAGCCTTCCTTCCTGGGCATGGAAA

TGGCTGGCATCCACGAGACCACCTACAACTCCATCATGAAGTGTGACGTG

GACATCCGTAAGGACCTGTACCTGCCCTGGCGGCCGCTCGAGGGCGTACT

TCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTAGTG

AGGGTTAATTGCGC

>HP35_SSH_trimmed.fasta.Contigl l

ATGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAGG

GCAGGTACAGACAGTCGACAACCATGTAGAGGTTCTTGTTCTCGTAGAGG

TCGTTCACCTGGAAGAGGTCCTGGCTCTTGAGGCCGTAGCTCTCGCACCC

CTTGAGGAACATCTCCAAGTTCTCCCTCTGTTTGAATGGCGCTTTCATAG

TGTTGATTCGCTTGACTGCGCCTGGCTGGAGGCAGTTCAACAACGTGCAG

AGGGCGATGCCGTCTTTGAGGGCATGCCCGAATGCCAGCTGGTCGGTGAG

GCCTTCCGCCGTGGGGAACTCCAGGGGGCATCTTCGTGACTCCCTCCACC

CAGTCGCACGCCTCTCGCGCTCCGTTGATGTCGAACTTGGCCTGGCTCTT

CATCTGACATTCCCTGCTGAGACCGTAGGAGGGGCCGTGGTACCTCGGCC

GCGACCACGCTGGGCGTACTTCTGTCGTCTTGAGCGTACTGATGGTACCC

AGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGC

>HP35_SSH_trimmed.fasta.Contigl 2

TGAGCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGCCGA

GGTACCTGGTGTTGAGACCATCTGTGTTGACAAGCTGAATCTGCTGAAGC

TTGCTCCTGGTGGCCATGTGGGAAGATTCTGTATCTGGACTGAGGATGCA

TTCCGTAAGTTGGATGCTCTCTATGGAACATGGCGCAGGGAAGCTAAGAA

TAAGAAGGGATATAATCTACCGGTGTCAAAAATGACTAACACCGATTTGT

CCAGGCTCCTGAAGTCGGACGAAATCAAGTCTGTCATTAGGAAGCCAAAC

AAGACCATTGTTCGTAGCAAGGTGAAGCCAAACCCACTGACCAACTACCA

AGCCATGCTGGAACTTAACCCCTTCCACCGTGTTGAGAAGAAATTGGCCC

AAGCTGTTGAAGCAAAGCACATTGAGGCTAAGAAATCTGGAAAGCCTACT

GAAGTTGTAAAGCCTGCAAAGAAAGAAAAGCAAAGAAAGAAGAAGACTAC

CAAGAAGACTGTGAAGAGGCTGAGGAAGCAACACCCAAGGAGTCATTCTG

GCAAGAAGGGCAAGGCTGCTGCAAAAGTAAAGCAAACCAGGCAATGCTTT

GATGAAAAATAAACCGTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAGTAC

CTGCCCTGGCGGCCGCTCGAGGGCGTACTTCTGTCGTCTTGAGCGTACTG

ATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGC

>HP35_SSH_trimmed.fasta.Contigl 3

GAGCCAAGCAGGGTACACGCACGAGTACGCCCTCGAGCGGCGCAGGCAGG

TACTCTGCGTTGTTACCACTGCTTACCGCGTACTTTΓTTTTTTTTTTTTT

TTTTTGGAAAACTACAACAAATACTAATACAATTACTCAAAGTAGAAAAC

TTAATATATATACTTTCATTCTATTGTCTTGTAACACCTGTAAAATTTTA

GATCTTCTTATAATGAAGGAATAAATTCCCTGTCCTCCGGTAATATTCAG

ATCACCCTATATCTCCAACTCTCTGATAAGTTTCTCCTCTTCCTAAAAAA

TTCTTACTTGCCCCATAAGTAGATAAAAATGGTATAAATCATACTAGAAC CAGAAAATACAACTATATTATAATGTTTTAAAGAATTTAAATTATAATTA

ACAGGCATTTAAATTTAGTAAATAGCCTAAAGAATACCTCCAACTGCTCT

AACAATTAAAGCTAACATCTTAAAAATAGGACTTATACCAAATTATATTA

GGACAGGGAAAAATCAATCAAGCTAATGTAGCTCCTCCAAATACAGCTCC

AACT

>HP35_SSH_trimmed.fasta. Contig 14

GATGAGCCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGC

CGAGGTACCATACAGCTGTAGCGAGGAGGAGTTGTTGATTGTTCTTGATA

GCACCGAGGATGGGTTCACCGAGGAGGAAGTTAGTGATTAAGCCACCAGC

AAAAATGGTGATCATGCTCGACAGCCAGTTGGCCAGGGGATGTTTCCGGG

AGAAGGGAAGTCCGCCGGTGCCGAGGTCCTCCCTGACGTAGAGGCAGCAG

AGGGCACAGTGGGCGACGTCGAAGTATAGAAACATCTTTAACTTGGTGAC

CTGGTTGGCCATCTCTAAGAAGGCTTCGGGATCCATGTTTGCGGGTGTGT

TCTTCCTCGGTTGGGGGAATACAACTCTTCCTCTTTCGTTTGTTTGTTAT

GACCTATGTATCTTTTTATTTCTGGACACCCACGAGGAATACGGGAAAGA

GAGAGAAGGGGAGAAAGGCGTACAGGTCTTTGGGTGTGGGAACAAGACCA

CTGTAAGAAGGACACGATGGTACCTGCCCTGGCGGCCG

>HP35_SSH_trimmed.fasta.Contigl 5

GAGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGTCGCCAGG

CAGGTACCTTGCATGCCAACGGAGCCTCTTTCTTTTTCATTTGTTTATAT

ATACACACAGGGCGGGGTATCTATTATGGGTCTTTCTTATATTTACACGC

CTGATCTGTAGGAGTCATTATTCTCTTACTTGTTATAGCAACAGCTTTCC

TAGGGTATGTACCTCGGCCGCGACCACGCTGGGCGTACTTCTGTCGTCTT

GAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTG

CGC

>HP35_SSH_trimmed.fasta. Contig 16

GTGAGCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGCCG

AGGTACTATAGTGTTAAATAAAGTCTGATCTCAAAGCATGCTCTACCAAT

CTACAGATATCAGTATATCAGATTTTCCTTTAGTCCTAGTCAGAAATTTG

GCTTGGAGGGGAAGCTGGTATCACCAATTTGTAAAGGTGTTCATTTGTGA

GCCAATGGTGTAGCACTTTACTCTGCTTCCGCACTGTCATATATAGTTTC

TCTGTTATCAAGCTGACAGTCAATCATGTCTGGGATCCAGGGGAAGTAAA

ATAGCAACCAAAAATATTGGGATACAAAAAGAAAAAGAAGAAAAAAAAAA

AAATTAAGGAAACTAAAAATCTGAGACTATTATCAATTAAAAATTCATAT

AATATTTCCAATAAGTATAACATTACCTATAAGTAATCACCTAGCAATTC

TCTCTCACAGGTTCACATTATATAAATATTTTCAACAAACCAATCATTCC

CCCTTTGCTCCTTCTATGACTACATGGCTGATCAATCACAAGCTTTCTCT

AAGGGAAGATAAACTATTAATGCATGTTTTGGAAACAGTCCTCTCTCGCT

TGCTATCATCACAGCTGATACTAAGATTACTGGTCCTCCAAATCCTTGGC

TCCGTGCTTGAGGATGCGAGTGAACTCCACATAGTCAAACATGGAGTTCT

TGATGGGGGGCTTCTCGGTACCTGCCTGGCGGCCGCTCGAGGGCGTACTC

TGCGTTGTACCACTGCTTGGAGCTCATT

>HP35_SSH_triπuned.fasta. Contigl 7

TGAGCCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGCCG

AGGTACGCGGGATTTTTCATCTATGCAGAACTCTGATTTTTTCAATATCT

CATTGTTTCTTGTGTAGAAGAAGAGTTCTGTGAAGATGACAGTCTCACTG

TTTGGCAGAAAAATATATTTCACTATTACAACATGAATAGAAAAAGTCTG

TCTGGGTGTTTGTGCCAACAAATAGGTTTTTTTTTGTTTTTGTTTTCTCA

TACCAAGTTGGACGACATTGGGCCTAGACTAATGGTGCACAAGGTATATC TTGCAGTATTGTAGTTAATGAAAGAAAGATAATTTCATGAACATAGGCTC

CTTGAAAAGTAAATATGTTTCAGAAAAAAAGTATTTTGCACACTTTGGGA

GTGTCAGATTCATGTGCAAGTTCTCGCAGAAAATACAAGAAAGAATATAC

AGAAAATATCTGGTTATAGGCATACAGTGTGGCAAGTAGGTAGATATACT

TCCTTTTTATTCTTTCATACCTATGATAAGCAACCTCCAAAGTTGTCACA

AACTTTCAGAAAGTGAACAAGGGGAATTCCATTTTGGTATTACAAGTTGA

AATATTGTAAAACTGCCATATGGGTCAGAACTGCTAATGAAGTGCAGGTT

ATGTTTTGAG

>HP35_SSH_trimmed.fasta.Contigl 8

GCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGGTACCATCAGTACG

CTCAAGACGACAGAAGTACGCCCAGCGTGGTCGCGGCCGAGGTACGCGGG

AAGCAGTGGTAACAACGCAGAGTACGCGGGAAGCAGTGGTAACAACGCTG

AGTACGCGGGGGGTTGGGTCGGCGCCGCGTCCTGCAGGCGTCTGCCTTAT

CAGCTCTCGATTGTAGGTTAAACGCCTACAATGGCTATCACGGGTAACGG

GGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT

CTAAGGAAGGCAGTAGGCGCGCAAATAACCCACTCCCGGCACGGGGAGGT

AGTGACGAAAAATACTGTTGCGAGCCCCGAACGGGGCCTCGCAATTGGAA

TGAGTACCTGCCTGGCGGCCGCTCGAGGGCGTACTCTGCGTTGTACCACT

GCTTNGAGCTCATC

>HP35_SSH_trimmed.fasta.Contig 19

GATGAGCTCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCA

GGCAGGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCGACAATCTCTTTA

TTAATTCTGGTTACACAAAATGCAGATTATTGTCTACATGAAAGCTGAAA

ATTCTATCCAATGATGTGTCTTACTAGAACAGTTTAAAGAATTTTGAAAA

CTATTCGACATATCCTAATTCAAATCAGAAGAGCCAAATGGAGGTCAGGT

CGTTCGCCAAAGGTCAGTGCCGTCACATGAGTCCTTCAAGGAGCGACAGT

TGCCCATTCCTCAGAAGATTCCTCAGAGCTCTCACTCTCGGAGCTGGAGG

CTTGTCTCCCGAGGCGCCTCAGATCCAAGTCGTGAGTGGCAGCGATGTCC

CTTTTCTCTATTCTCTCAAAAGACGCGCTGTCGCTGGTGCTCCCTGTGCT

GATGTCTCTCTTCTCTATCCTCTCCACGGACGTGCTTCCGCTCGTCCCTG

AGCTCATGTCCCTCTCGACGCGATGGAGCTCCTCGTAGACGTCGCCGAGG

GAGTAGGCGGAGGAGAGGCCGACGACGGCACCCCCGGCGTACCTCGGCCG

CGACCAAGCTGGGCGTACTTCTGTCGTCTTGAGCGTACTGATGGTAC

>HP35_SSH_trimmed.fasta.Contig20

TGGCGCGACGAAGGCGAGTTAGGTTAACAACGCCAGTAGTTACTGCCCTT

CGAAGCGGACCGGTCTCTAGAGTTCTAGAGTTACAGAGCCTCCCCCTAGC

GTAATATTGCCCTTGACTGACCCCACGCGTGACAAACCGGCTGTCTGATC

GTACCCCAGTGTACCATCTTATGAGAGCTTGCCTATCCCATCGGAGGGAC

CACATGGAACACGCGCACGGNTAAATGGGTCAGTATCGTCCGAAAGACTC

TCGGGCTGAATCGTATAAAGTATCTGACTGTGACTCAATGTCTCACATGT

CCGACCGAGACAGTAGTAGTCACTGTGCAGAGTCAATCACTGTCTCTGGC

TCGGGCAANTGTGACAAGTACTCGCTATAATTTTACTCGTCACTGACCCG

AGCGTCTTCCGGGACGGTTAATCCCAATTCCCGGTGCGCGTGGTTCCAGA

TTTGGTCCCCNCNGATGGGATATGCAAAGCCTTCTTATAAGATAACCATC

ACGGTGGGGATNCACTCCGGAACGGAGCGGTGTTTGTCACGCCGGTTGTG

GGTTCGGATCCACAAGGGAGAACCCAATTATAATCGCNTTAGGGGGGAAG

GGTCTCCAGATACTTCCCCATTAGAACTCCGNCAGTAGGAGTTCTCTGAG

GCCAACAGGCCTTATCCGAAAGAGGGACCCGGATTAACCTTACCGTGGCC

GGTTTTNTGGTTTTTTTTCCANCCAANTTTTCTGGCGCTTCTTCTTGGTT GGGGGAACAAGCCACAATCTGNCGCCATAAAATTCCCTCCTGCACATCTC

CCTTATTAATNATAATGTTTGCGCGAATNGTTNTANCTNTACAAACACAG

GTNTGNAAAACCGTGGGTNCAANCCTTGTTTCCCTCTTTCCGGAGAGTCC

NCTTGGCTGGACCCTCCTGTGGGATGAAACGGGTCTTTTGTTTTTNATAC

ATCGNGAAATTNCGGGTGTTTTTCCAACTTCCCGGCCAAAACGTTTTTGG

TTGTTNTTCANGAGGGATAATCCCCCCCCCCCTTTGGTTGGTTCNCAGGG

TTTTTGGACAACCGCCGGGCGGATTTATTCTCTTCATTTTACAATGATAG

GTNTGCCGGCGGCCCGGCCCTTTTTTNTGTGGCGCGGACGCGGGGCCTNA

ACCTATATTTCCCCGCCTGTTCCGGGCGGGCCGTTTTCCTTNATTCCCNA

GGGGCCCCCNTAAGGGGTCCTTTGGGCCGGCCCCTCTTGGGGGTTTTTAG

GCGTTTTTTTGACGCACAAGGCCCCAGTNCTCNGACGGCGGTTCTAAATG

GTAGGNTCCCCCCCCCGGGGCTGCCGATTAAACCGACNCGCGGAGAATAT

TTTTTCCTTGGGCCCGCCCCACACCGTTAATTTTCCACACCCAGACNACA

AAAAAATTATCACAAAACCGGGATGTAAATATGTGGGCCCCCCGTGCGCC

CTGAAGGAGGACACGGCCGNTTAGGAATTTTAAAAAAAATTTTTGAGGGG

GGGTNCCGNGGGGATGTAAAAAAAANTGNTTAATGGGCGGGGCGGNCCAA

TANCGGGGGGCCGCGGGGGCCTCTTCAGGNCCGTTCGGTTTTTAAAAAAA

CGTCTCCGGGGAGAAGGGT

>HP35_S SH_trimmed.fasta.Contig21

ATGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAAGCGGCCGCCAG

GCAGGTACACTTCAGAATGCAATGGGGACTGTGCTGTTAACTGGGTTTGA

GATGATGCAGATCATAGCATTGGGGCAATTTTCAGCACAAGCCTTGGCCA

AGTTTGCAACAATAGAGGCGTTAGTGTTGAAAAGATCGTCTCTGGTCATG

CCAGGTTTGCGGGGAACTCCAGCAGGGATGGCGACAACTTCACATCCCTT

CAGGGAATCTGCAAGTTGGTCGGGTCCAACATAGCCGGTGACCTTGGCTG

GGGAGTTGATGTGGGAGAGGTCAGCAGCTACTCCTGGCGTGTGCACAATA

TCGTAAAGGGAGAGTTGGGTGACCAAGGGAGAGTTCTTCAGGAGTAGGGA

GAGTGGCTGACCAATGCCTCCACTGGCACCCATGACAGCAACCTTCCTGT

TGGCGGCATTGGTGGTGGAGAAGTTCTTGACCAGCTGACCAAGGACATTG

GCAGTCTGGGGCTTGGCAAGTCTGGAAAACATGATTATTGGTGTTGAAGT

GCCGCTGGTGTTCTCTCCCCCGCGTACCTCGGCCGCGACCACGCTGGGCG

TACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTT

AGTGAGGGTTAATTGCGC

>HP35_S SH_trimmed.fasta.Contig22

GATGGAGCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGC

CGAGGTACATTGACCGCAATGGAGAAGAACCACGTCTTCCTGGTCTCGAC

CAATACTCCCCCCAACAGATTTTCTTCCTTTCTAATGCCAACATCTGGTG

TGGTTCTATTACCAAAGAGGGTCTTCTGAACCAGGTGCTTACTGACCCCT

CACTCCCCAGGCAAGTTCCGTGTACCTGCCCTGGCGGCCGCTCGAGGGCG

TACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTT

CAGCTCACGGTTAATTGCGC

>HP35_SSH_trimmed.fasta.Contig23

ATGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCGCCAGGG

CAGGTACTGGACGTTAGGGCTATACAAAGAGGATTCAATAATATCTCCAA

GGACGTCGATTCCACGCTCATTCATGATGTCAATGTGATTGCCAGCCTTA

TCGACAATATAACCGTGGGCAATAGCATCGCGAATGCGGCTCTCAATAAT

AAGCAAGTCTCGAATGCGAGCAACACCGTCCACATCCTCGAAGTCTACAT

TATCTGGACGAGAGGGGAACTGACCGCCATACTTGTAAGTGGTGTGGGGA

GCAAAACCTTGTACGATGGGTTTACCCCAGTGAAGTTCGTCGACGGGATC CAAGTAATTAGAGAGACGTTCGGCATCAAAACGAACGGTAAGTTGATGAT

GTACCCAGAAGAAGTTCTCTCCTTTGCGGTCCAGGTGATGACTGTAACTG

TCTTGCCACCAGAATGGGAATTCCATATGCCAGGTAACGTGGTGAGTGTT

CATTCCAATGTCCTCTCCGAAATAGGCTACTCTCTGTTCAGGATTCTTTT

TAGTTCCAGTGAAAACCTTAAAGACTTAAATTTACCAGGTTTTGTGTCTG

CTTGGCACGATAGCTG

>HP35_SSH_trimmed.fasta.Contig24

TGAGCCAAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCGCCAGGC

AGGTACTGCTCGTAAAACTTATTGTGTCCGACCCAGTGTTCCTTATCGTG

AGCTTCTTCCGTCACTAGGACAGTCTGCCTTTCTCCCAATTTATGATCAT

ACGGTCTATACGACTGGAATAACTCTGTCAGCTGTCTTGAGCGATTTTTC

ACCTCTTTTGTAGGTATTCTTTGCATCTTGGCCGCTGGAGTTCCTGGCCG

TGGATAAAACTGATTGATGAATAAGGATGGGAACTTATAACGTCTACACA

GTTCTATAGTTTCCTGGAAATCTTTCTCTGTTTCTGTAGGAAAGCCACAG

ATTATGTCTGTTGCTACTGTGATACCTGGGACTTTTTCCTTCAAAAAATC

TACCACTCTGTAAAACTCTTCCACAGTGTATTCTCTTTTCATGTCAGCCA

GGACACCATTACTTGCAGCCTGGACTGGAACGTGCAAGAAGGCATAGACT

CGAGGATGTTGCATAATCTTGGCAATTTCCTCTAAATGGTCTAAGATGTA

AGGTGGGTTTGTCATGC

>HP35_SSH_trimmed.fasta.Contig25

GATGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAG

GCAGGTACTTTTTTTTTTTTTTTTTTTTTTTTGTGGAAAAGATTTTATTA

CAAGAAACAGGTTAACTGTGAACAAGGGAGTCAACATGTTTTTACATGAA

TTTAGAAAATAAAAAATAATAGTTACAGATTCTGGCAATGAAATAAATAC

ATCAAAAGTCACACTGACAATATCTACAAGATTTGTATATTTATGAGCTT

ATAAAATCAATTGCCCTAGCCTACATGAACAACGATTTCTTAGGGTAATA

TATGAAAGATTCTTTTCTGTCAGACCTTGAAAACCATTAAAAAAAAAGTT

TAGAACAAAAATACAATACTCATAATTTGAAAGAGCACTGAACCAGTAAG

AATTTTGGAAAAAAGAAAAAGGGGGAAAAATAGAGCATTAATCTGGTGGT

TGCATTTGCACGATTTGTGATACTTCAATTTCAATCATGAATTGCTTTTT

AAGTCAATTTATCTCATTAAATGTCTTTGCTAAACCTGCTCAAACTATCA

CTTTCATT

>HP35_SSH_trimmed.fasta.Contig26

TGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAGGG

CAGGTACGGCGGCCGAGACGCAGTGCCCCACGAATACCCGTGGAATGTGG

TCGTGTGGATGGACCATGCGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT

GGCTACTGAAAATTTTATTATAAATTGTAAAAAAGATAATATATCACAAA

TCTATGACCCAATAGGTTATAGATCACTCTTCAGTATCTCTTATAGTAAT

TGTAGTTTCCTCAGGGGATAATGCACAAAATTTCTTCGTGAAGCATAAAT

ACAATGTCAAAGACCATGTGATATTCCATCAAGAAGGGACAAATTCAAAG

GAATTAGGTATTCTTTGGAAAACTCTTTGCCAAGTTTAAAAGCACACTTC

ACATAGACATGGAGCACAGGTATGATCTTTCATGCTTCAAATGATGATAA

CATTACTACCAGAAATATCTTTAAAGTACCTCGGCCGCGACCACGCTGGG

CGTACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCT

TTAGTGAGGGTTAATTGCGC

>HP35_SSH_trimmed.fasta.Contig27

TGAGCTCAAAGCAGTGGTGACAACGCAGAGTACGCCCTCGAGCGGCCGCC

AGGCAGGTACTGGTGTGCCCTGATGGGAGAACTCTGGAGTCTGAGGCTGC

CCATGGCACTGTCGCACGCCACTACCGCCAGCACCAGCAAGGCAAAGAGA CATCCACAAACCCTATTGCCTCCATCTATGCCtGGACACGAGGTCTGGCC

CACCGTGCCAAGCTGGACAAATAATGAACCCTTGTCCAAGTTCTGTGCAA

CGCTTGAACAGGTGTGCGTAGACACCATTGAGGGTGGCACGACATGACCA

AGGGACCTTGCGCCCAATATTGGTATCAAAGGGGATGGCAAAGCATCACC

CGCAGTGACTACCTCAACACCGTTTTGAAGTTTCTTGGGGACAAAAACCT

GGCTTGAGGAACTTGGCAGGAAGGACAGATCACTGCCTTGACTATCCTTG

GCAAGGAAACCAGTCACATCACACTGCCAGGCTCTAACATGCAACAAGAC

ATTCCCCAGCCCCTTTGTCTAATATTAAAAATTGTGTCCCAGTGTCCCTG

GGGGATTTTATTGATTCTGGTGCTTTTGAAAATATTTTAAACTTGTATTA

GGGACCAGATAATAAATTTAGTAAATTTTGAAAAAAAAAAAATAAAAAAA

AANAAAAAGTACCTC

>HP35_SSH_trimmed.fasta.Contig28

GTGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAGG

CAGGTACTAAGATAACTTTAATAAAATTAAGAGATAGAAACCGACCTGGC

TCACGCCGGTCTGAACTCAAATCATGTAAGGATTTAAAGGTCGAACAGAC

CCTCCTTTATAACTGCTGCATTATAAGGAAACCTTAATTCAACATCGAGG

TCGCAACCCTTCCTGTCGATATGGACTCTCAGGGAAGATTACGCTGTTAT

CCCTAAAGTAACTTAATCTTATGATCTCTAAAAGAGGATCATTAATTTTC

CAGAAATTACTGTTATTAAATAACTTAAGAACAGTTACTCATTATATTCC

CGTCGCCCCAACGCAACAAATATTATCTAAAATCAAGTTATACTAACAAT

TTATAATATAAATAACTTATTGTAAAGCTTTATAGGGTCTTATCGTCCCC

TTAAATTATTTAAGCCTTTTCACTTAAAAGTTAAATTCAATAAACCCCGC

GTACCTCGGCCGCGACCACGCTGGGCGTACTTCTGTCGTCTTGAGCGTAC

TGATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCGTC

>HP35_S SH_trimmed.fasta.Contig29

ATGAGCTCAAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCA

GGCAGGTACGCGGGACAGCCAAAACGCCTCGTTCCCACTCGCCATCGAAG

TCCTCGACCTGAGCTACAACAACTTCAGTCTCCTCCCGAGGAACTGCTTC

CGCCATCTGCCGGCGCTCAAGAAACTGATCCTGGCGCACAACCCCCTCGG

CGACATCTCCCACACGACGAGCATGGCCATCAACGAGCTCCACAGCCTCG

TCGAACTTGACCTCTCGCAGACCGGGATCGAGCGCCTACCACACGGGTTC

CTCACTGACCTCCTTAACCTCCAAGTGCTGATCCTTGCGGGGAACAGGCT

CACGAGTGTGCCGGAGGAGGTCAACTACGCTCGCTCGCTCACGCACCTCA

ACCTCAACGCCAACCCCATTGAGATCGTACCTCGGCCGCGACCACGCTGG

GCGTACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCC

TTTAGTGAGGGTTAATTGCGC

>HP35_S SH_trimmed.fasta.Contig30

TGAGCCAAGCAGTGGTACACGCAGAGTACGCCCGCGTTGGTCGCGGCCGA

GGTACAAGCTGTCAACAGTTGTCTGTCTCGTCTATCAGATCCTTATCCTT

CTTTCAGTCGAGGTGCGTGTGTGTATGCGTTTTAATTTCCAGTGTATATC

TAAAGATTATCGAATGTATATGATTAAAAATCCTTCAATGGAATACATAC

ATACCACAATGCCCTTCTTGGATTAGACTGTGCGTATGTGTACCTGCCCT

GGCGGCCGCTCGAGGGCGTACTTCTGTCGTCTTGAGCGTACTGATGGTAC

CCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGC

>HP35_SSH_trimmed.fasta.Contig31

GATGAGCTCNAAGCAGTGGTACACGCANGTAGATACGCCCTCGAGCGGCC

GCCAGGGCAGGTACCTACAGCAGATTCTACAGATGATGAAGATGTAGAGA

AACAGCTTACAGCAGATAATTTGGCACTGATCGTAGCTAATGCAGTAAGT GAGGACCAAGCAGTTCAGTTGTCTGCAGTTCAAGCAGCACGAAAACTGCT

CTCTTCAGATAGAACCCCCCTATTGATGATCTGATCCAGTCTGGAATCCT

TCCTATACTTGTCAAATGTCTGGAAGGCACTCACAATGTATCTTCCCCGG

ACAGCCCATCCTTACAATTCGAAGCGGCCTGGACTCTGACAAATATAGCC

TCAGGAACATCCAGGCAAACTCAGGCGGTGGTCCAAGCTGGAGCTGTTCC

CCTATTTCTTAAATTACTTCAGTCATCTAGCCAGAATGTCTGTGAGCAAG

CTGTTGGGCACTAGGTAATATAATTGGTGATGGGCCACAACTAAGAGACT

ATGTCATCTCCTTGGGTGTTGTCCAGCCACTTCTTAGCTTCATTAACCCA

GAAATACCTATTGGATTCTTACGAAATGTTACATGGGTCATTGTCAACCT

GTGCCCGGAACAAGGAAACCACCACAACCCAACAGACTATAAAGGAGATT

CTTCCTGCATTAAATATCTTGATTCATCACCATGACACTTCTATATTGGT

GGACACAGTATTGGGCTCTTAGTTACCTTACGGATGGTGGTCATAATCAT

ATTCAGTGGTCATAACGTGGTGTTGTTCCAAAGCTTATTCCATTGTTCTT

CCCTTAAGAATTAGGTCCAGAACGCGCTAAAAGCTGTGNACTATGTACTG

GACTTCGCCGGAACCAGCCTGGTCTCGTCCTTNNCCCCTCTCTNTGTCCC

TTTTGTCCCCTGAAAGGTATCCCCNGATATNCANCTTCCTGTAAATTTCC

ACACNCACANTCGC

>HP35_SSH_trimmed.fasta.Contig32

GATGAGCCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGC

CGAGGTACAGAGAGCCAGCATGCATCTGCTTGTTGCTTAATTTGTAGACT

CCATTCACAGTAAAGGAGTTTTCCCAGTTGAACCAATCATTGAAGGTAAT

GGTATGGTCAATAACAACAGATTTTGTTCCCTTCATGAGAACTATGTTGG

AAGTTCTTGTGTTGTCATCCTTCTTGCTATCAATGCTGACTTTTACATCA

TCTAGGCCACTGAAAGTGGAATTGACTTCAGCTTTAAGGATTACCAAGCT

CCTAGACACATTCAGAAGTCCATCCACAGGCACATGAATACGTTCAGAGT

TGAAAATGACATCAACTGAGGAATCCATCTTGGTGTTAGTGACAGTGTGC

TTGAAGGCAATTGATCCCTTATTTACTGGGCTAGAAACGTCAACCTTGAA

TTCTACATCATTCTTGGAGAGCATGTGTCCCTGAGCGTGCACCTTGACGC

TACCGTATGTAGACCAGGATCCTGGTCATCTCGAACTGCGATGTAAAGTG

CTCCGTGTCATGGGTGTGCTTGAGAGATACTGTCAGAGGGTAAGTGAAAC

GGCAGTAATGAAGCGAACGTCTCCAGAAAACTGTCCGTAGTGTCCGCCAC

TGACAGTCCTCTTGGCCACTTACATTTAAGGCATGATTTTCTTTCTTCCC

CAATTCTTTACCTTTCAGTGATCACAGTTTGGAGAACCTTTCTCCACTTG

GAGAAGAGTTCTTTTACACGNATTGGGCCTTTTNTTCTTCAGGGGATCCA

CAGAAACACGCGAGTGTCACCTTGAACCACCAGAGTGACTTNCTTGTTAC

TTGCCCTGGCGGCCGTCGAGGGGTACTCTGGTCGCTTGAGCGTCTGGTGG

TACCCAGTTTTGTCCCTTAGTGGGGTATGNGCGCTCGCGNCACCTGCTAG

TCGTGTGGAAATTTCGGTCATCC

>HP35_SSH_trimmed.fasta.Contig33

GCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGGTACCATCAGTACG

CTCAAGACGACAGAAGTACGCCCAGCGTGGTCGCGGCCGAGGTACGGCCC

CCAGGAAATGCCCTGTGCCCCAGGAACCGTGTGGGACCAGGACCTCTTGA

CCTGCAACCACGAGGCCACGACCCCCTGTGTCATCGCCGAACCCTGTGAC

AAAAGTCATGACAAGCAATGCCGCAAAGGAGACGGAAGATATGCCGTCGA

AGGAAAGACAGCATATTACCTCTGCCGCTCCCGAGTATCCTTCCTCCTTC

AGTGTCCCGATGAAGGAACCTTTAATCCTCACATTACATCCTGTGTCTAT

AAGGACCGCACTGCCATCGGGAAGGTAGTCGCTTAGAGAAAGATAAGGGT

TGTGGGTTGTGTAAGAGTTAAATAAAATGAAATTTGAACAAAAAAAAAAA

AAAAAAAAAAAGTACTGTTCATGAATGAATTACATCAGCAGCTCTAATTA GAACTCGAATTTGAGTATTTATTGGTAACACAGTGCGATTGTCTACATCT

AGAAGCCGGAATCCGTTCTCAGGCAGCTCATTGGAAGGAATCATATAAGA

ATCAAATTCTACTTGAAGAAAGTCTGAATATTCGTATCTTCAATATCATT

GATGACCAATAGTTTTTAATGCTACTCTAGGATTATTAACTTCATCTAGT

AAGTATAAAAGTCG

>HP35_SSH_trimmed.fasta.Contig34

GATGGAGCTCAAAGCAGTGGTACAACGCAGTAGATACGCCCTCGAGCGGC

CGCCAGGCAGGTACCACCCACTCCCGTCTGCATAACGAACGAGACATCCA

GCGTTAACGACACAGACCTGTGCGAGCGCCTGACTTCGGTCCTGGACGAC

ATCGAGGCCATTACGAGCACCGTGGCCGAGGGAACCATGGGCCAGACCGA

GTTGGACGACCTGAGGAACTGCTCCACGAGGCTTGACGATGTGATTGAGG

ACATGCGCAACAACACAGACTTCCTCGCCAGCAGCGTTGACGTTGACGGG

TCTGATTGCGAGGAGAGATGCTCTCGACGAGGTGCTGACGGAGGCCGAAA

AGGCAGTGGAAGACGCGCTGACGACAGATGACTACGACCCGACACTCGCC

ATCGTTCTCGGTGTTCTGGGAGGCCTCCTGGGCGTCGGCATCATCGGCTA

CGTTGGTTTCGCTTTCTATAAGAAGAACAAAGCCAAAAAGGCACGCAAGA

ACGATAATCCTATGACAAGCATGGAGAGAGGTTCAGGGCAAGACAACAGC

GCATATGCCGGCAGCAGCAGCAGAATCAACTAGGAAATAAGAGGAGCATA

TAGTATAAATAGTCTCATGCTTTTGACTTTTACTTTTCGAAAGCCTAATC

ATATGTTCCGTCTTGTCTAAGGGTGAAACGAAAAAAAATAAAAGAAGACT

TAACAAACACGATTGCGCTGAAGGCAGTAAGTAGAAAAGGATTAAATACA

TGATAATGATAAAAATAATACTGTCCATGAGATGAAAAAAGTATATGTAA

GGCATGTTTTAAGTATAAATAATACGCCTCACCTCATTACGATTAAAAAA

AAAAATGAATCGGAGTGCCCCAGAGTACCTGCCTGGCGGCCGCTCGAGGG

CGTACTACTGCGTGTACCACTGCTTGGCTCATC

>HP35_SSH_trimmed.fasta.Contig35

TGAGCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGCCGA

GGTACATCCTTACATATGGCCTATCAGGATCATACTGGCTGGGTGCCTCC

GATCAGTCTTATGAAGGTGACTGGCTGTGGGTAGCTGATAACTCTAGGGT

TAACAAGGGCACACCCTACTGGGCTATCCACAATGGAATCTTTGGCTGGG

CTCATGAACCTGCAGGTGGTGCTGACGAAAACTGCCTTCTGCTTGATGAA

AATCGCAAACATTACTTTAATGATGCCAATTGTAACCTGATTCATCATCC

CCTTTGTATGATTTAAATGTAACCATAAATAAAGCTTGGCAATGAAAAAT

CCAAAAAAAAAAAAAAAAAAAAAAAGTACCTGCCCTGGCGGCCGCTCGAG

GGCGTACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCC

CTTTAGTGAGGGTTAATTGCGC

>HP35_SSH_trimmed.fasta.Contig36

GATGAGCTCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGC

CGAGGTACGCGGGAATTTAAGGGGACGATAAGACCCTATAAAGCTTTACA

ATAAGTTAtTTATATTATAAATTGTTAGTATAACTTGATTTTAGATAATA

TTTGTTGCGTTGGGACGACGGGAATATAATGAGTAACTGTTCTTAAGTTA

TTTAATAACAGTAATTTCTGGAAAATTAATGATCCTCTTTTAGAGATCAT

AAGATTAAGTTACTTTAGGGATAACAGCGTAATCTTCCTTGAGAGTCCAT

ATCGACAGGAAGGGTTGCGACCTCGATGTTGAATTAAGGTTTCCTTATAA

TGCAGCAGTTATAAAGGAGGGTCTGTTCGACCTTTAAATCCTTACATGAT

TTGAGTTCAGACCGGCGTGAGCCAGGTCGGTTTCTATCTCTTAATTTTAT

TAAAGTTATCTTAGTAGCCTGCCCTGGCGGCCGCTCGAGGGCGTACTTCT

GTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTAGTGAGG

GTTAATTGCGC >HP35_S SH_trimmed.fasta.Contig37

ATGAGCCAAGCAGTGGTACACGCAGTAGTACGCCCAGCGTGGTCGCGGCC

GAGGTACGTGGGCACCATCGGAGGCAAGCAGAGGGTCTCCCTGGACTCCA

ACGGCTGCATCTACAAGGGCACGGCCATCCACGAGCTCATGCACGCCATT

GGCTTCTACCACGAGCACTGCCGCAACGACCGTGACTACTATGTTACTAT

TCACTACGAAAATGTGCAGTCAGGCATGGAGTCTCAGTTCAACAAGGACA

CCTACTGGCAGTACCTGCCCTGGCGGCCGCTCGAGGGCGTACTTCTGTCG

TCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTA

ATTGCGCG

>HP35_S SH_trimmed.fasta.Contig38

TGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAGGG

CAGGTACTGGTTGGCGTTATTCACTTGAACTGAAACACATTTTGATACTT

AATTCAATTCATTGTTCTTACTGTCTCCTACTTCTTTCACTTGTAATAAA

GTGTTTGTGTCACTTCTATTACACAATCATCTACACCAACTCATGCATGT

GACCAGCAGAAGTAATGGTTAAGCAAATTTCATAAAATTAAATTTATTAT

ATAACTAATAATAATAATAATAGTGACACTAACAACAGCAATTACAACAA

TAACAATAGCTAATTTTGTAATGCACAAGGAGCCATAAAGCAACCATTAT

ATTGGCACTGGATATGGTGCTAACTGGAACCTTGTAAATCAACATCCCAG

ATAATTCTGCTGTAATCTAATCCCTCATCATGATTACCACTATTGATCAG

TACCTCGGCCGCGACCACGCTGGGCGTACTTCTGTCGTCTTGAGCGTACT

GATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGC

>HP35__S SH_trimmed.fasta.Contig39

GCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGGTACCATCAGTACG

CTCAAGACGACAGAAGTACGCCCAGCGTGGTCGCGGCCGAGGTACGCGGG

GGGGCCGGCCGCACACATCTTGGTGAATCAGAATAACTTTTGCCGAGCGC

ACGACCCCTCCCGTAACCCGGGTTGGGTCGGCGCCGCGTCCTGCAGGCGT

CTGCCTTATCAGCTCTCGATTGTAGGTTAAACGCCTACAATGGCTATCAC

GGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGG

CTACCACATCTAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGGCA

CGGGGAGGTAGTGACGAAAAATACTGTTGCGAGCCCCGAACGGGGCCTCG

CAATTGGAATGAGTACCTCGGCCGCGACCACGCTGGGCGTACTTCTGTCG

TCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTA

ATTGCGC

>HP35_SSH_trimmed.fasta.Contig40

GATGGAGCTCAAAGCAGTGGTACACGCATAGTACGCCCACGTGGTCGCGG

CCGAGGTACGCGGGGATCGGGAAACGAAGGGCAGTTTCCCGTCTCCGAAC

GAGAAGCACTTGACATTTGAGTTGAGGAAAGGAAGGGGAGGCTGAGTCAG

AGGGAGAAACGGCCAGAGAAACAACAAACCAGAGTCCATTGACGTAACTA

TAGCAGACTTCGATAGTGTGTTGTATCATCTATCAAACATCAATGGTGAC

AGGACAAAAATACGGATAAGCATCTCATTAAAGTTCTACAAGGAGCTCCA

GGAACATGGGGCGGACGAACTCGTACCTGCCCTGGCGGCCGCTCGAGGGC

GTACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTT

TAGTGAGGGTTAATTGCGC

>HP35_SSH_trimmed.fasta.Contig41

GCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGGTACCATCAGTACG

CTCAAGACGACAGAAGTACGCCCAGCGTGGTCGCGGCCGAGGTACTCGTT

GCCGTCGGCATTAATCTCGAAGTTAACATTGGCCTTGTCCTTGTCTGCAT

AGATACCCAGGTTGGATTTCCAGTCGAAGTCAGACGTCTTGAGGAATCAC

ATCGACGGATGGTGTTTGATTTTTACCGGCCACGGAATTATCCTTAAAAG TTCCTTCCAAAACCATTTCTTTCTCATTAAAACCACCATACACCTGAGCT

TTGTAGTAGCCAGCTGCGATCTTATTGAAAACAAATTCAAGGGAAGACTT

CAGGTCCTCAGCCTGCACGTTGATACGTCCGAGTGTTTCGACGTCATACT

GATGACTGAATTCCACCCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAG

TACCTGCCCTGGCGGCCGCTCGAGGGCGTACTTCTGTCGTCTTGAGCGTA

CTGATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGC

>HP35_SSH_trirnmed.fasta.Contig42

GTGCCAGGTGATTAACGCCCTCAAGCCCGGTCAGATCAAGAAGATCCAGA

CCTCCGCCATGGCCTTCAAGTGCATGGAGAACATCAACGCCTTCGTGGAG

GGAGCTAAGGCCTGCGGCGTGCCCACTCAGGAGACCTTCCAGACTGTCGA

CCTTTGGGAGCGACAGAACCTCAACTCTGTTGTTATCTGCTTGCAGTCTC

TGGGAAGGAAGGGATCTCAGTTTGATAAGCCTTCCATTGGCCCCAAAGAG

TCTGAGAAGAACGTCCGCCACTTCACCGAGGAGCAGCTCAGGGCTTCTGA

GGGCATTGTCAACCTGCAGTATGGCTCCAACAAGGGTGCCACTCAGTCTG

GCATGTCCTTCGGCAATACTCGCCACATGTAAAAGCAATCTTTGTACCGG

ACGTCCCAAGCATAAAGGACCTGTTTGCAAAAGTCGAGGCAGGCGGCACT

GGCCTTTCCGAATTCGAAAGTGCAACAGGCCTGCCAAACAGGTTCCTTCT

CCCCAAGGGCAACGATAGAGGCCTGGAATTCGACCTTGTGGTGGCGGTGA

CCGATGGTGATGCCGACTCAGCAGTGCCGAACCTTCATGAGAATACCGAG

TACCTGCCCTGGCGGCCGCTCGAGGGCGTACTTCTGTCGTCTTGAGCGTA

CTGATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGC

>HP35_SSH_trimmed.fasta.Contig43

TGAGCTCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAGG

CAGGTACGCGGGAGTCTGACGACATTTCATGACTTCCACAAGCAAGAGAC

TGAAGATGGAACGTTCGCCTTTGACTCCCTTGTTGATCCTGGTCCTTTGT

ATGGCGGCAGGAGCATGGAGTCAGACACTGCTGGATCCTATAAGCAGAGC

CTGTCGCGGAATGGCGGTCGGGAATCAGGCAAAGGCGCTGTCCAGGAACG

AGTATCCTGCAGGAGGGCTCCTGATGGTGCTGGCTTCCGTGCCCGCCGGG

GTCAAGGGCTTCTTCAAGTTCTACCTGTGTCTGGACGACGACGCTGGGCA

GGAGGAGTGCCTCAACAAGATGTCCCTCCCGTTGGCTGACGGGTCCGGCG

ACACTTTCGACCTGAGCAAAGTGGCGAAGGGCGGGCAGTTTCGAGATCCC

GTTGACCATCCCCGATGACGTGACCTGCGACACCTGTTCTGTCCAGTGGA

CATGTGGAGGTGGAGGACTGTGGAGGGAATGCAGGCTGCTCACTCATAAG

CCAGGATTCCTGCTTTGACGTCAAGATCCGTCAGGCCAAGAAAGAGGAAA

AGAGGCTGTTTGGACTTATCTTTGGTGCAGTAAGAGCCATTGGAGGCGGT

ATCAGGGCCCTAGCCAGGAGGAGAGGCTAGAAGGTGGTGTAACGACCTGG

CTCGACTTGTCCCCCGACGTCACGCGAGGAAATGGGAATATGGATACTTG

TGGAACTCCGAGGTGTTGAGAACTGGTGCCGAGCAGACTTCACCACGAAA

ATAACTATTTCATTGTTGTGATATTCATACAATTGGATTGTTTATAGTCA

CAGAAACAGGGCCATGTGCAGGGAGTAAAAAAAAAAATCGATAGAATTAC

AGTTTTATGATGCAAAAAAAAAAAAAAAAAAAAAAAG

>HP35_SSH_trimmed.fasta.Contig44

TGAGCCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAGG

CAGGTACTAAGATAACTTTAATAAAATTAAGAGATAGAAACCGACCTGGC

TCACGCCGGTCTGAACTCAAATCATGTAAGGATTTAAAGGTCGAACAGAC

CCTCCTTTATAACTGCTGCATTATAAGGAAACCTTAATTCAACATCGAGG

TCGCAACCCTTCCTGTCGATATGGACTCTCAAGGAAGATTACGCTGTTAT

CCCTAAAGTAACTTAATCTTATGATCTCTAAAAGAGGATCATTAATTTTC

CAGAAATTACTGTTATTAAATAACTTAAGAACAGTTACTCATTATATTCC CGTCGCCCCAACGCAACAAATATTATCTAAAATCAAGTTATACTAACAAT

TTATAATATAAATAACTTATTGTAAAGCTTTATAGGGTCTTATCGTCCCC

TTAAATTATTTAAGCCGTTTTCACTTAAAAGTTAAATTCAATAAATATAA

TTGAGACAGTTTGCTTTTTGTCCAACCATTCATACAAGCCTCCAATTAAG

AGACTAATGATTATGCTACCTTCGCACGGTCAGTATACCGCGGCCCTTTA

AAATAAATCAGTGGGCAGGCCAGACTTTACATACCAATCATATAGACATG

TTTTTGATAAACAGGCAAAAGCATTATTTGCCGAGTTCCTTAATTATATC

TTATATCAACTAAAAATTCTTTCCTTATTTTAAATTGTTACTACACAACT

TATTTCTACTAATAAAATCATTATTACTTTT AATGATATATTTATTAAAA

GAGACAGATAATTATTACTAAACCTTCACTATATAAATATTAATTAATGA

ATTTACATTAACTAGAACGCTTTATTAATATGGCTGTTTTTAAGCCTAAT

TCAATAATACTTAAATCCATTTCGTTATAGTAAACTTATTATTAGAATAA

GAAGCTCTTCCCTCCTACTCTTTATTACTTTAAAGCTTTCTTCGACCTCA

AAGCAACTAAATTTAATTTAATTCTCTATCAACCAGATATGAAAAATCGA

CTAGCATTTCACTACTAATATAAATTTTATAATTTCATTAATACGAAAAC

TATCTATTTATATTAGCTCTTTCTCTTTCGGGATTACTTATCTTATTAAG

ATTATTTTGATTTTCCCTAATACAACAAGTACCTGCCTGGCGCCGCTCGA

GGGCGTACTCTGCGTTGTACCACTGCTTGAGCTCATC

>HP35_SSH_trimmed.fasta.Contig45

GCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGGTACCATCAGTACG

CTCAAGACGACAGAAGTACGCCCAGCGTGGTCGCGGCCGAGGTACTGCCA

GTAGGTGTCCTTGTTGAACTGAGACTCCATGCCTGACTGCACATTTTCGT

AGTGAATAGTGACATAGTAGTCACGGTCGTTGCGGCAGTGCTCGTGGTAG

AAGCCAATGGCGTGCATGAGCTCGTGGATGGCCGTGCCCTTGTAGATGCA

GCCGTTGGAGTCCAGGGAGACCCTCTGCTTGCCTCCGATGGTGCCCACGT

ACCTGCCCTGGCGGCCGCTCGAGGGCGTACTTCTGTCGTCTTGAGCGTAC

TGATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGC

>HP35_SSH_trimmed.fasta.Contig46

GTAGTACGCCCGTGGTCGCGGCCGAGGTACCTGAAAATAATTCTAATGTA

ATGCCCAAGATACAAAATACAAACAAACACCTCATAACTTGTATCTTAAA

CATCTCCGAGGCATAACAGGATGAGATGATAGTTGTGCTGCATGCACTGG

TCACTGTCAACTTGAAGTCCCATAGGGTATACTTTTAAAGGGTTATTATT

GGGACAATTATCGAGGACATGAGGACACTCGCACCAAGGGTCTTTCAAAC

CCTCCTTGATATTTTTTATAATTAAAATTACATTTTACGAATCTGAAGAA

CATAAATAAAATCTATTTTCAATTACATTTTACTATTATTCTGAATAAAG

GAAATAGAATCTGATGGAAAATACAAGACATTTTGTTACGGTTCATTTTA

TAGTGTATGTGAATTTATAATAACTGTGGCTATCTGTAAAAAGTGAATGC

ATTCATTTCTTAGCAAAGGTTTTGTTGTAAGAGACTGCAGAGTGTTAATG

ATATCTTATATGTCTTTGCATCGCTACGAAACCCTCATAATTTTCATCAG

TAAAAGATTGTCAATGGCCAAGAATGTTTGGTTACTTCTCCGTGAACTGC

AGGTCCTTTAAAAGTATCTTCATGGCACTACACGAGACATGCTGGGCAAC

TTGCCCGCGTACCTGCCCTGGCGGCCGCTCGAGGGCGTACTTCTGTCGTC

TTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAAT

TGCGC

>HP35_SSH_trimmed.fasta.Contig47

GATGAGCTCNAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGG

CCGAGGTACAGTCACTACCTGCCTTACAGGTGCAGGCTCCGCTGGCAGCC

CAGTGCTTGCAGTCTGCATCCTCGCCACCGCATCCACCTGGCGGTCGGAC

GGTCACAGTTGGGCATCCATTGCAACCTGAACAGCTTCCATTGGGTAGAA GGTGGTTCCCTGTCACACAAGGGGTGTTCCATTCATGQTTGCAGGTGAGG

ATCTTCTGGTCCCACCTGGTTCCTGCAGGGGCATGGCATTTCCCGGGGAC

CATAAGGGGCACACTGAATGAACTTTTTGGCAGTCGGTAGGATGCTGCCA

GTATTGCTGGCCAGTGGAGCAATCTACACAGGGGAAGTTGCCTGGTGTGA

CAGGTTTGTGTGTTGGAGGGCTGGTTGGGGGCTTTGCTGGAGGAGTGCAT

CCTGAGCATCCTGAACAGCTTCCGTTAGTGAGAAGGAAGTTGCCTGTCAA

GCACTCAATGGCCCATGCATGGTTGCAGGTGAGCAGGTTTTGGTCCCACA

CTGTTCCTTCTCCACATGGCATCTCCTGAGGACCGTACCTGCCCTGGCGG

CCGCTCGAGGGCGTACTTCTGTCGTCTTGAGCGTACTGATGGTACCCANG

CGTTTTTGTTCCCTTTTAGTGAGGGGTTAATTTGCGCCGCTTTGGCGGTA

ATCATGGGTCCATAGCCTGTTTCCCTGTGTGAACATTGTTAT

>HP35_SSH_trimmed.fasta.Contig48

TGAGCTCAAAGCAGTGGTACAACGCAGAGTACGCCCTCGAGCGGCCGCCA

GGGCAGGTACTTTTTTTTTTTTTTTTTTTTTTGTGATTTCTTATGGCTCA

CTTTTAACTTTAGTTACAGATTGCCAATAGTGGCAAAGATGATATCACAT

GAAAATTTTAACAAATGTATTGCCATACAACCTCTATGTCTTGAAGATCT

AAGCCAAAAGAATGGACAGAATCAACAAGCTTAAACCATTGAATATAAAA

TGATATACTTTATAAAAATTATTTTCTATTGCCTCTTCTTCCTACAGATG

ATATTAATGACTTCTTTAACCTTCCATTCTTTCCTTGTGCTTAATATTAG

TTCAAGTGTTATTATTAACATATTCATGTGCAGTTGTACCTCGGCCGCGA

CCACGCTGGGCGTACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCT

TTTGTTCCCTTTAGTGAGGGTTAATTGCGC

>HP35_SSH_trimmed.fasta.Contig49

GGAAACAGGCTAATGGACCCATGGATTACGGCCCAAGCCGCCGCAAAGTT

AACCCCTCCAACTAAAAGGGGAACCAAAAAGCCTGGGTACGCAATCAGTA

CGCCTCAAGACGGACCAGAAGTACGGCCCTCGAGCGGCCGCCAGGGCAGG

TACTCATTCCAATTGCGAGGCCCCGTTCGGGGCTCGCAACAGTATTTTTC

GTCACTACCTCCCCGTGCCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT

CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT

GATTCCCCGTTACCCGTGATAGCCATTGTAGGCGTTTAACCTACAATCGA

GAGCTGATAAGGCAGACGCCTGCAGGACGCGGCGCCGACCCAACCCGGGT

TACGGGAGGGGTCGTGCGCTCGGCAAAAGTTATTCTGATTCACCAAGATG

TGTGCGGCCGGCCCCTTGCGGGAACCGGCTGCCGAGGGTTTTGGTCTAAT

AAAAGCGCTCCTCCCTCGCGGTCGGAGACTACAAAGGCATGTATTAGCTC

TAGAATTACCACAGTTATCCAAGTAATGCGGTTCAGCAGAGATCCAATGA

GTTATATCTGATTTAATGAGCCGTCCGCGGTTTCGCCTTAAGTCGGCCTG

TACCTCGGCCGCGGCCGCTCGAGGGCGTACTTCTGTCGTCTTGAGCGTAC

TGATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTTGCGC

>HP35_S SH_trimmed.fasta.Contig50

AAGGGAACAAAAGCTGGGTACCATCAGTACGCTCAAGACGACAGAAGTAC

GCCCTCGAGCGGCCGCCAGGGCAGGTACGCAGGCTAGAGTCTCGTTCGTT

ATCGGAATTAACCAGACAGATCACTCCACCAACTAAGAACGGCCATGCAC

CACCACCCACCGAATCGAGAAAGAGCCTTCATTCTGTCAATCCTTCCGGT

GTCCTGGCCTGGTGAGTTTCCCCGTGTTGCGTCAAATTGAGCCGCAGGCT

CCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCA

ACCATACTTCCCCCGGAACTCAAAGACTTTGGTTTCCCGGAAGCTACCCG

CCGGGTCATGGGAATAACTGCGGCGGATCGCTAGTCAGCATCGTTTAAGG

TTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCT

TGATCAATGAAAACATCCTTGGCAGATGCTTTCGCAGTAGGTCGTCATGC GACGGTCTAAGAATTTCACCTCTAGCGTCGCAGTACCTCGGCCGCGACCA

CGCTGGGCGTACTTCTTGTCGTTCTTTGAGCGTAACTTGATTGTGGTACC

CCAGCTTTTTGTTCCCCTTTTAGTGAGCGGGTTTAATTTGCGGCCGGCTT

TGGGCGGTTAATTCCATTGGGTCCATAGGCTGGTTTTCCCTTGGTTGTTG

AAAATTTGGTTTATTCCCGGTTCCAACCAAATTTTCCCAAAAACCAAAGC

AATTAACCGGAAGGCCCCGGGNGAAGGCCATTTAACACAGAGNTTGGTTA

CAAACAGGCCCCTTAGGGGTGGGGTTGGCCCTTAAAATTTGGAAGGTTTG

GAAGGGCTTTAAAACCTTTCCAACCATTTTTAACTCTCTCGGCCGTTTTG

GGCCGGGCTTCCAACCTTTGGCCCCGGGCTTTTATCCCCCAAAGNTCCNG

GCGGGGGAAAAAAAC

>HP35_SSH_trimmed.fasta.Contig51

GCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAGGCAGG

TACTACACTTAGAGGAAGTGTAAACGAGACGAAAGAAGCTGAACTGTAAT

TGGAAAATCCTACCCCAGCTAAGATGAGTAGGAAAAGAACTGCCTTCTGA

AATGATATCTGATACAGACACAGTATCAAGTGTTATGGGTTAAATAGGTT

TCCATTCACACCGTGAATCTACTACTTGTGTAGTTCTTTAGTGGCTCAGA _{ττττττττττττττττττττττττττττπ}

TACGCATTTTTGGAATTTCTCTAAACTGGGATCTCATTGCAGGTACGGTC

CTCAGGAGATGCCATGTGGAGAAGGAACAGTGTGGGACCAAAACCTGCTC

ACCTGCAACCATGCATGGGCCATTGAGTGCTTGACAGGCAACTTCCTTCT

CACTAACGGAAGCTGTTCAGGATGCTCAGGATGCACTCCTGCCAGCAAAG

CCCCCAACCAGCCCTCCAACACACAAACCTGTCACACCAGGCAACTTCCC

CTGTGTAGATTGCTCCACTGGCCAGCAATACTGGCAGCATCCTACCGACT

GCCAAAAGTTCATTCAGTGTGCCCCTTATGGTCCCCAGGAAATGCCATGC

CCTGCAGGAACCAGGTGGGACCAGAAGATCCTCACCTGCAACCATGAATG

GAACACCCCTTGTGTGACAGGGAACCACCTTCTACCCAATGGAAGCTGTT

CAGGTTGCAATGGATGCCCAACTGTGACCGTCCGACCGCCAGGTGGATGC

GGTGGCGAGGATGCAGACTGCAAGCACTGGGCTGCCAGCGGAGCCTGCAC

CTGTAAGGCAGGTAGTGACTGTACCTGCCCTGGCGGCCGCTCGAGGGCGT

ACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTA

GTGAGGGTTAATTGCGC

>HP35_SSH_trimmed.fasta.Contig52

GCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGGTACCATCAGTACG

CTCAAGACGACAGAAGTACGCCCTCGAGCGGCCGCCAGGGCAGGTACTGG

TGAGCGGGCACACACGTATCCTGTTCCGGGTGGAATACTAGCCCAGACGA

GCACGTGAAGTAGGTAATGTTCTTGTTCACATCACAGTAGTAATAGGATG

TGCAGTCAGTCGGGTGAGGGATATGGCAACATTCGCATGTGCATCTGGGG

TCGACTGTGGGAGGTGGGCCAGTGGGAATTGGGTCGGGTTTAATTTCGCA

ACTCTGGTGGTCCCCTGAAGGTTACCTTCGNGGCCGCCGACCCACGCCTT

GGGGGGCCGNTTTANAACCTTTTTCCGTTTGGTACCGGATTCCCTTTTGG

AACGCCCGGGCTTAAACCTTTTGGAACTTGGGCGTTAAAGCGCCCGCCCA

AAGGGCGCCCTTATATATΓCTCTGGTTTTCCCCCCCGCTTTTTTTTAGGN

TTTGGGNACGGCGGGGGATTCTTTACANCACATTTTCTGGGCCCGGGCCA

CTGGGCCCTTTTTGGGGAGGCCGCGCGG

>HP35_SSH_trimmed.fasta.Contig53

ACGCTCAAGACGACAGAAGTACGCCCAGCGTGGTCGCGGCCGAGGTACAA

CCGGTGAAAGCGTCTTGAAAAGGGCGTACTAGGCCGTCAAGGTGATCCTC

ATGGAAAGTTTGATTTACCACCTGGTGTGCTGGAACACTTCGAAACTGCC

ACCCGTGATCCCAGCTTCTTCCGGCTTCACAAATATATGGATAACATTTT CAAAGAACACAAGGACAGTCTACCCCCATACACCAAGGCCGATTTGGAAT

TCTCTGGCGTGTCTATCTCAGAGCTAGCCGTTGTAGGTGAACTGGAGACC

TACTTTGAAGATTTCGAATACAGTCTTATCAACGCAGTTGATGATGCTGA

AGGAATCCCAGATGTGGAAATCAGCACATATGTGCCTCGTCTTAACCACA

AAGAATTCACTTTTAGGATTGATGTAGAGAATGGAGGTGCTGAGAGATTG

GGCACAGTTCGTATCTTTGCCTGGCCTCATAAAGACAACAACGGAATCGA

GTTTACATTTGACGAAGGTCGCTGGAATGCCATCGAGTTGGATAAGTTCT

GGGTAAATTTGAAGGGTGGAAAAACTACAATTGAGCGCAAGTCCACGGAA

TCTTCGGTAACTGTACCTGCCCTGGCGGCCGCTCGAGGGCGTACTTCTGT

CGTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGT

TAATTGCGC

>HP35_S SH_trimmed.fasta.Contig54

CAGAGTTGATGATGGCATTCACAACAACCTGGACTGGGTTCTCTCCAGTG

AGGAGGTGGATGATCTCGAAGGAGTGCTTGACAATGCGGACGGCCATGAG

CTTCTTGCCATTGTTGCGACCGTGCATCATGAGGGAGTTGGTCAGACGCT

CCACAATGGGGCACTGAGCCTTGCGGAAACGCTTGGCAGCATAACGACCA

GCAGAGTTGGGGCAAGTACTGGTGAGCGGGCACACACGTATCCTGTTCCG

GGTGGAATACTAGCCCAGACGAGCACGTGAAGTAGGTAATGTTCTTGTTC

ACATCACAGTAGTAATAGGATGTGCAGTCAGTCGGGTGAGGGATATGGCA

ACATTCGCATGTGCATCTGGGGTCGACTGTGGGAGGTGGGCCAGTGGGAA

TTGGGTCGGGTTTAATTTCGCAACTCTGGTGGTCCCCTGAGGTACCTGCC

CTGGCGGCCGCTCGAGGGCGTACTTCTGTCGTCTTGAGCGTACTGATGGT

ACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGC

>HP35_SSH_trimmed.fasta.Contig55

GCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGGTACCATCAGTACG

CTCAAGACGACAGAAGTACGCCCAGCGTGGTCGCGGCCGAGGTACAAGGT

TTTGCTCCCCACACCACTTACAAGTATGGCGGTCAGTTCCCCTCTCGTCC

AGATAATGTAGACTTCGAGGATGTGGACGGTGTTGCTCGCATTCGAGACT

TGCTTATTATTGAGAGCCGCATTCGCGATGCTATTGCCCACGGTTATATT

GTCGATAAGGCTGGCAATCACATTGACATCATGAATGAGCGTGGAATCGA

CGTCCTTGGAGATATTATTGAATCCTCTTTGTATAGCCCTACCGTCCAGT

ACTGGTGAGCGGGCACACACGTATCCTGTTCCGGGTGGAATACTAGCCCA

GACGAGCACGTGAAGTAGGTAATGTTCTTGTTCACATCACAGTAGTAATA

GGATGTGCAGTCAGTCGGGTGAGGGATATGGCAACATTCGCATGTGCATC

TGGGGTCGACTGTGGGAGGTGGGCCAGTGGGAATTGGGTCGGGTTTAATT

TCGCAACTCTGGTGGTCCCCTGAGGTACTGGTCCAAGGACACCTCCTGCC

AACCGCCCATCATGCCCACGTAGGACCAACAGCCTCCGCCACTGACGCCA

TTCGTAGTGATGTAGATGTAGTTCGATTCCCGCGTACCTGCCCTGGCGGC

CGCTCGAGGGCGTACTTCTGTCGCTCTTGCAGCGTACTGCATGGTACCCA

GCTnTGTTCCCTTTAGTGAGGGTTAATTGCGC

>HP35_SSH_trimmed.fasta.Contig56

GCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGGTACCATCAGTACG

CTCAAGACGACAGAAGTACGCCCAGCGTGGTCGCGGCCGAGGTACTAAGA

TAACTTTAATAAAATTAAGAGATAGAAACCGACCTGGCTCACGCCGGTCT

GAACTCAAATCATGTAAGGATTTAAAGGTCGAACAGACCCTCCTTTATAA

CTGCTGCATTATAAGGAAACCTTAATTCAACATCGAGGTCGCAACCCTTC

CTGTCGATATGGACTCTCAAGGAAGATTACGCTGTTATCCCTAAAGTAAC

TTAATCTTATGATCTCTAAAAGAGGATCATTAATTTTCCAGAAATTACTG

TTATTAAATAACTTAAGAACAGTTACTCATTATATTCCCGTCGCCCCAAC GCAACAAATATTATCTAAAATCAAGTTATACTAACAATTTATAATATAAA

TAACTTATTGTAAAGCTTTATAGGGTCTTATCGTCCCCTTAAATTATTTA

AGCCTTTTCACTTAAAAGTTAAATTCAATAAATATAATTGAGACAGTTTG

CTTTTTGTCCAACCATTCATACAAGCCTCCAATTAAGAGACTAATGATTA

TGCTACCTTCGCACGGTCACCCGCGTACCTGCCCTGGCGGCCGCTCGAGG

GCGTACTTCTGTCGTCTTGAGCGTACTGATGGTACCCAGCTTTTGTTCCC

TTTAGTGAGGGTTAATTGCGC

>HP35_SSH_trimmed.fasta.Contig57

CTGGGGCCCCCCCGGAAAATGTGTTGTCCCCAGAAGTGTGTGAAAAAATG

TGGCCAAGGCATTGGGGCCAAACGGAACCCAAGGGGTTTTTTCCCCGGAA

CCTTGGGGATAAAAAGCCGGGGGCCCAAGGTGGAAGCCGGCCATAACCGG

CCAAATTTATAATTGGTTGGAAGGTTTTAAGGCCTTCCAAACCCTTCCAA

TTTTAAAGGGGGCTCAACACCCCCAAGGGGCCTGTGTTTAACCAACCTGT

TTTAATTTGGGCCTTCCCCCCCGGGGCTTTCCGGTTAATTGTTTGTTGTT

GGGAAAATTTGGTGGAAGCCGGGAATTAACCAAAATTTTCCAACCACCAG

GGAAACCAAGGCTTATTGGACCCAATGAAGTTACCGCCCCAAGCGCCGCA

TAATTAACCCTCAACTAAAGGTAATAAAAGCTGGGTACCAGTCAGTACGC

TCAAGACGACAGAAGTACGCCCAGCGTGGTCGCGGCCGAGGTACTTTTTT

TTTTTTTTTTTTTTTTTTTTTTTTTAGCTTTTCCGAGATGCATTGCAAGA

AGAGTTTAAAATTTTGATAAAAAACATAATCATCAGAAAACAATATCATA

AAAAATAATATCAAGGATGCAACTTTTCCAAAAAGAAAAAAAAAAAAGAA

AAAAAAATTTGGTCGTGCTTTTTGCCAATTCGTAATTCTTAAAGAAGGTC

TTGAGGACGTTTCACTGTAGTTAATGGAAGATTTCCGTCAGTCATGTGTC

GTTCGGATCTAAAATCCTGGCCTAGTTGCCCGATCGAAGCCTTACGTTCG

CCGACTGCTGCAATCGACGTTGCCGGCATGGTCGCAGGACTTCGTTTCTT

GGTTCCAGAGGAGACCGGAGGGGCATTCCTTCTTTTCGGGCGTACCTGCC

CTGGCGGCCGCTCGAGGGCGTACTTCTGTCGTCTTGAGCGTACTGATGGT

ACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGC

>E:\AnalyzedData/50513_2_2_Ql_Kubota_Hpl4_02Run01_Cp312_MDl/Q2_Kubota_Hp35

_02_08H01.F.esd 965 MegaBACE

GAGCCAAGCAGTGGTACNNCGCAGANTACGCCCAGCGTGGTCGCGGCCGA

GGTACCGCATCCAGTGTTGCCTTCACACCGCTTCAGGGTCTTGAGATTGT

CAATCCTCAGGCAGCAGAGATGAGCGGACATGCTAAGGCCAACAAGTATT

TCTCTAATGTTCTTGGATTCAAGAATGTAAATAACAAATTGGTGTAGTTG

AAGATGAAGCAAGTGAATGGAAACATATTTTTTGTAAAAGGAGAGTAAAT

GCAAGTTACCAGAAATATTATGTGTTACAATGGATGTAATAAAAGAATTA

GTTGTATTAATTTTATTTTACTTTACAAATTACCTACATATAGTGTATGT

GTGTATGTACTTTTTTTTTTTITTTTTTTTTTTTTTGCAAAAGCACTTTA

TTGGCTCCCAAAATCNCATTTACTTGGATTCCCCAAAAAGCAAATTAATT

TTGAAACCAAGGCCAGATAAAATTCTCTTATTAAACNCTTTAAAAATTCC

TGTTAATGCTTAACCATTAAATGCCAATTACAATGGGGTTNAACACATTG

AGACTCACACATTACAATCCTGGCAAATTACACAATTCNTTGTACCTGGC

CTGGGGGCGGTCGAAGGCGTACTTCTGTCGTCTTGAGCGTACTGNTNGCG

CCAGTTTTGTTCCCTTTAGTGAGGGTTAATTGGGCXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGCCGG GGAAGGGGTTTGCTTTTGGCGCCTTCTCGTCTCGGCCATGAGNCCTGGCC GNTTCGGTGGGACAG

>E:\AnalyzedData/50513_2_2_Ql_Kubota_Hpl4_02Run01_Cp312_MDl/Q2_Kubota_Hp35

_02_22F03.F.esd 1472 MegaBACE

GAATGAGCTCAAAGCAGTGGTACAACGCANTAGATACGCCCAGCGTGGTC

GCGGCCGAGGTACCGCGNGCGAGCTACTCCGAATTCAGTATGNCACTAAA

TAGATGACATCCACTATGCGTGCAGCGTATCATCAAGAAGCATTGTTGTC

AGTAGTCGTCGCTGATCTTCATATTCACTTGTATGGCACCATTCAAAGTA

CTCATATTACTGACATCGANACTGACTGAGTAACCATAAGTATAACCAAT

GTGAGCNCAGAGTTAGCTGTAAGACNGGAACTGTAACACATAGTGAGACC

ACNGACATACACATCGAAGTGTNATGACACAAAAGGAGAGATTATTAATG

ACGAAGGGGCAANCAATAGTTAAAATACAANATGCGCGTCANGCAATCGT

CTAAGAAATTAAAGAAAGGATCAGGTATTAGTCTGTGGAGCCATGTATGT

GTGGCTTACATGCTCAACTGTATGAGTAAGCACAGAAACTTGTGGAAGTT

GACTCTGCCAAAATGTGTCTCCGAGGTGTATATGNAACTGACAGTCGTTG

NACCGGGTAATCTCTAACTTGTGCTTAATCAAAGTCGCTTCAACNAAGNT

ACTGTCTCCTAATCTGTTTCTACCTGTTGTTTATGCACTTTGGGGCCGAA

GGGTCACTTTCGCTTTGTGTTNGCAGGGTACTATGCCTATTTCCAGTTCA

GGGACTTGATCCATTTTGGGGGCCTATCGGCTTGTTGCTTTNGGTACCCA

TAGGCACACCTAGAGNCGGNGCCCGGCTTCCGGCAGCGGGGCCGGATTNA

CATCCTGCATTGTACTGCGTCCTTATGGAAGCCCGGTCCTTGGTGGATAN

CGCNNGACGTGGGTTGGTGCCCCCATCATATGANGGTCGAATGANTCGTC

CCAGGGTCAAGGGCCGACCGCCTGTGTCGAGGGCAGNATTTCCACTATCC

CTTATAGGGGNACCATAAAGGCAATGGGTAAGTCCNCTTAGTTAGTTGAA

NAAAATTTTGGTCTTTCCANNGGCTCTAACGAAACGTTTTCCAAANCCAN

TCTTAATCCAGTAAAACANGAACNGCTTCAGAGTCCAAGTCCAATTGATT

ACCGCGGGTTCANTACAGGGTCCCTTTAGAGGGGGGTTGGCCCCATACCA

GATAGCAAGTTGGAAANTTTAAAAACTTCAACGGAANTTTTAAAAATCTA

GACCAGTACGGCAATCTTGCCAGCCTTGGGACACACCGGNCCTTGTTCNC

CAGNTATCCGAANGGCAAGAATCCCNGGTTCGGTGGTCCCATCTTGGNCA

CTTTTAAAATTGTAAAATTACCCTGGGCCCCCATTGCGCCCTGTGGGAAA

AAAGACTCAGTTTTGCACGGGACATTGGTTNGCACACCTGTCACACAAAT

TAACTAACGGCTTACAAACATACATTCTGAAGAATGCACAGANACCAAAA

CTGNTTTCCGGGATGTAGCCCG

>E:\AnalyzedData/50513_2_2_Ql_Kubota_Hpl4_02Run01_Cp312_MDl/Q2_Kubota_Hp35

_02_26B04.F.esd 903 MegaBACE

GATGGAGCTCAAAAGCAGTGGTACACGCANTAGATACGCCCTCGAGCGGC

CGCCAGGCAGGTACAAAAGTGTGCTTTTTTTTTTCCTTTTCTTTTCCTCC

TTTGTCAGTAATTTGAGACTTCTTATTGGTGAAGCTACTTTTTCGTTTTT

TTGACCTATATTATAGAATGCCAAGTCGAGATTGATATTGAAATAGAAAA

TGCAAAAGAAGAAAGATTACGAGTCAAACCGGGAATGTTTAACAGAGTAG

TTGAATAGATGGAAGAAAGGAATCTATTATTGTATTAAAGAGAACGCAGT

TTTTATCAGAGTAATTTTGTCTATGATTCTTATAAGTTAGAAAATCTAGA

TTTTTGAACTCTTGTATCTTTGCATGGAACAAAGCCAGTTAATGTATTTT

GAATAATTAATGTCTCAGAGAATGTTCTTTTTTATATGCAGATTTCTATT

TTAAATCGTATGTTGATGCATAGTCCATGTCTGTAATAGATATGTATCAT

ATGATATTGATATTACAGTATTTGTTTTACAAGTATTCAATACATCCAAT

AANAAGGTCAATTCATTCTAATTGTTGTCAAATTTTCACGGAATGTCTCT

TTCACATTTGGTTCACAGTGCTACAACATGAGCAAAAAAAAAGAGACCTG

AATTATCTTCGAGTATATGTCCAAATTATATTGACTNTTTCTAAGGTGTT TTATGTGTACTTACTTTTGAGTACCCTCGGCGGGAACCAACGCTGGGGGT

ACTTCTGTCGTCTTGAAGGCGTACTGATTGGTACCCAGTTTTTGTTCCTT

TAGTGANGGTTATTTGCGCGGCXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTTGAGCCGGGGGGCTAAG

NGT

>E:\AnalyzedData/50513_2_2_Q l_Kubota_Hp 14_02Run01 _Cp312_MD l/Q2_Kubota_Hp35

_02_34B05.F.esd 1401 MegaBACE

GAGTGGACTGCCCACAGACAGTANAGTGACAATCAGCATGAGCTANCACC

GCGTTAATACGGACTCACTTATAGGGCGTCAGTGCTGTGTCAGNCTCTGC

GATCGCTCGACGCACANCGAATTCGACCTGGCAGGTCGGGACGGGTCCAC

ATGTTGGGGCATTACCNTNTGTCGACACCCTTGTANACCATCTTCCCATT

GNGGTATCAAAGGTGGTTCCCTGTGTACNCGACAACGGGGTTGTTCCTTC

TCTTGGTATGCTGGGTGAGTCGATCTTCTGGGTCCCACCTGGTTTCCTGT

CGATGGGCCANTGGNATTTTCCTGCGGTGACCCAGTATATGGGGGCGATC

GACTTGATACTGACANCTTTCNGCGCACGTTCGTGTCACACGTGACTGCN

TGCCCTAGGTAATTTTGTCTGTGCCAGGATCGCGATGGCCAGATGTTCGT

AACCCACCATGGGGGGTAGCAAGGTCTTGCCCCTTGGGGNTGTTTGCACC

GAGAGTTGTCACTTGGGTCGTTGGCTCTAGGGAACGTGTGGCCTTGGGTC

CNCGGGGTGGGCTTTCTGCTTNCGGACCGCGNAGTGGCGATTCCTTTGAC

NCCANTCCCGTAGGAAACCCAGGGCTTTTCCGCCGGTTATGTTGAACGCA

GAGGGGAGAGGTTTTGGCCGTAGTCAAGGCNAGCGTCCAAAATTGTGGCC

CCACTTGTCGANTTGGTTTACCCAGGAGCTGAAGCAATGAGCTTATTAGG

GTCNCCCACACGTAGTTTCCTTTCTTCCCACATAGGTCATTCTTCCATGC

ATGTGACCCTTGTTGATCCCTTNGACCGCGCTTTGAGCGAGCCCTGCTTT

CGGATGCGCGGCCGGTAACCTTTTCTGCTTCGTTCCTTTTGNAAGTCGGG

GTACCTTGGACCTTGTGGTANCCACACGGCNTTTCTTGGTTCCCCCGTTT

TTTAGTTCGACGGGCGGGTTNAGNTTGGCAGNCGTGTCCTTTGGTGGCGC

GGTTACACTCCANTCTGGNTCCCACTTAAGCAGTTTGGTTATTCTACCCC

CCTTTNGGTTTGGTTGGAGAAAATCTCCGGTTCAATTTACCGCCTGGGGC

TTNCGANCTAAAAATTTCCACCAACGCGACACCGAGCAATCCAGATTACC

GGGAANAGCCCCCCGNGGTCGGAAGCCACACTTAAANACGGNTTGGCTCA

ACACACCGGCGCCTTTTGGGGGGG AATTGCCCGGTTAATTTCGCACGTTG

GAACGCTTAAATCTTCCCAAGCAAATTTTTAAACATTTTGGCCGTTTTGC

CGGCNGTCAACCTTTTAGGACCAGCGGCGTTTTTCACCCACACGGTCTCC

TGGGGGGGAACAAACGCCCTTNGATACTAGTGTAGAGCACCNAAGGTTTC

A

>E:\AnalyzedData/50513_2_2_Ql_Kubota_Hpl4_02Run01_Cp312_MDl/Q2_Kubota_Hp35

_02_53E07.F.esd 1009 MegaBACE

TGAGCTCAAGCAGTGGTACACGCAGAGTACGCCCXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

XXXXXXXXXXXXXXTAACTTTCACNAACTGACGGTTTTAAATTCNACCTT

ACGTTTTTTGAGTTAACTTGGTCTGGCGGTTCCCGGGTTTCTCGTGNNGG

GTNCTCTCNTCCTCGCGCTGCTCTTTCTCGGNCTCACCATGNGACACCCC

GGGTGAGTAGAACCACAGGGGGNCTCTGTGCCCGTGTGNATAGTCGACCC

GGTNTTCAA

>E:\AnalyzedData/50513_2_2_Ql_Kubota_Hpl4_02Run01_Cp312_MDl/Q2_Kubota_Hp35

_02_73A10.F.esd 228 MegaBACE

TGAGTCAAGCAGTGGTAACACGCAGTAGTACGCCCAGCGTTGGTTCGCGG

GCCGGAAGGGTTACATTTTTGGGGATTACAAACTGGTAGTAACGGTATGG

TANTGGTAGACTAAGGGCCGGGTTTATTTTGACGGACTTTGTAAAAACTG

GCACTTAACTGAGTGAATTTGAATTTTCCTAACTATTAAAGGGGGGCCAA

AAAGGGGGGGCCCGGGTTTCAAAAAAAT

>E:\AnalyzedData/50513_2_2_Q 1 JCubotaJHp 14_02Run0 l_Cρ312_MD 1 /Q2_Kubota_Hp35

_02_92D12.F.esd 767 MegaBACE

TGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCGCCAGGCA

GGTACTGCATCATCAATAGCTCGTTGTCATTCCCTGATCCNCACTGCATN

TCNTGACNATAGTCACATTCAAATATGTCCCACATAGTACTTGGTATTGA

NCCTTCCTTACCAATTCCTAGTATTTNGTGATTACATAGCTCTTATGCAT

TGGTATACCTTACTGCGAGTTACCTTGCGGCCCGGCGACCACGNTTGTCG

TNGTNCTNNCGNCNATTAGCGTCNGGTCTTTGGTGCCCACGCTTCTTGCT .

TCCCGTTGACNCTTGAGCGCGCTGTAAGTCTGCTGCGACTTTNCGACAGT

AACTACTGTGGGTACATTACCTGATTGTCCTTCGCTGTTGAATATTCGCT

TTACTCCCTGTGTCCTTGGGAATTCTGCCTTCATACCCGGGGTTCACAGA

ACGGGCTGACGCATCGCGGGTTTACCGGGCCGGTNGGTCCAAACCGGGCT

NTCGTTGGGGGCGGGTTAGNNCCTTCCTAGTTGCAGGGTTCGTATGGCCT

TACCGTTTTACCTTCGATTTGGGTTATCGCCCGATTGTGCGTCTGTCCTT

GCATCCTCGCTTCTCGGACTTTGCCGCGGTTTCCGCGTGAGNCAGCCGTC

CTTTTCGGGTCGGCTCAAGTCCTCAGCTCGGTTTCACATTTAGACCTTTC

NTGTGGTCCCCCTTTGGCTTNGCTTTCCAGGCNCGTGTACAGGAAGGGNC

GGCGGGNTTTTCGGCCG

>E:\AnalyzedData/50513_2_2_Ql_Kubota_Hpl4_02Run01_Cρ312_MDl/Q2_Kubota_Hp35

_02_94F12.F.esd 562MegaBACE ^{" "}

ATTGGAGCTCAAGCAGTGGTACACGCATAGTACGCCCTGGTCGCGGCCGA

GGTACAAGGATCCCTGATTGGTTCCTCAACAGGCAAAAGGACATTAAGGA

TGGCAAATACAGCCAGGTCATGTCTAACAACTTGGACACAAAGCTCCGTG

AGGATCTTGAGCGTCTGAAGAAGATCCGTGCCCATCGTGGTCTGCGCCAT

TACTGGGGTCTCCGTGTGCGTGGTCAGCACACCAAGACCACTGGCAGGCG

TGGTCGCACTGTTGGTGTGTCTAAGAAGAAGTAAAAAACTAATACATCTT

GTAGGGAAATTCATTGTATTTTATTATCCAAACCTTTATTTGCATCGTAT

TACACAATTCCCCTTGATCATTTTAGTTAAAGGTTTTGGCTGAGTTTAAT

AATGTATATTGTCTTGCCCAGGTAAAATTTATTTGAAGTGTGCTGTACAT

TTCTCCTCAAACTATAGAACTGACCATGCTCTTACATTATGTTTACAGAT

ATACTGATTATAACTGATTATAGAATACTATACATGAAATTGTAATAANT

CGTGATATGAAA >E:\AnalyzedData/50513_2_2_Ql_Kubota_Hpl4_02Run01_Cp312_MDl/Q2_Kubota_Hp35

__02_96H12.F.esd 1568 MegaBACE

CNGAGTGGGAGCCCCCAACNGGCAGGTGCTACTAACCAATCTGCATCTNA

AATGACTGCCCTGATCAAATAACGTACTNCTACTAATAAGTGGACTNTGT

GTGCGATCGCGAGCTCCCGGGGCTAACNGAAGTCATTACATTTGCATCAT

TCAGTTCANNCAGGNTATACATCNTAGGTACGGGTAACCTGGGACNGCAC

GGACACACATTAGGCANNTNCCATTAAACGTACATCAGGCTCAGCGNCCT

TCGAAGATGCCCACTAGGACGCAGACTAAATGGCGTTATACTCGTTCGTG

ACGCTCGAAATGTGATGAANTTCGGCNCAATTACGTAACGGNCTAAAAAT

GATTTCTCTATGNGCATGCTGGGATCTTGTAACTGAATTGCTTTTTCATT

AACTTATGTTNTACGAACCCAACGTTCACTTACACAAGCAAGACCCATAA

TTACTCTACCATTAAAAAGCCCCGGGATTNATCTNCCTGCACTNNTAATT

TCACCTTTCCTTTTTACAAGCGAAATACCACTTAACCAAGTTGNCCCCGC

NNTTCCTATANCTTTGCCAACCCAGGAACCACTCCAATTTCGATTTCACA

CTTCCCCGAAAGATAACGCATTNCCNTTACCAAAGAGACATCACATCCCG

ANTTTCCTCTCCCCCTCACACACNTTGGGNCCCATTGTCCNGGAAAAACG

CANAGTTNCCNTTTTCAGCAANTTGTCACCCTCTGACACAAANTTAACTC

AGAATTTAAAATTACACCTTTATCCTANCCCCATTTGGCCCAATTTATTA

AGTCNCCCAATCTCTAATTAAATATTAACACCACACACGGCAANGGGGCA

CACATCTAACCAGANAAAGGGCAAAATTCGTTTCACCATTGGTTTCACCT

AAATCCNCGAATAAACGTGTTCCGCNGTTGGAACACCCACTTTTTNCGCA

TCCGGGGGAAAAATTTCCGGGGCTTTTTTCACTAGNCNCGAATTTTTTCC

CCCCTCTTTTAGAGTGNTCCACAAAGCAGAAAAACNANTACCGCTGTNTT

CCCCCTATTATTAAAAAGGCGGGATTATAATCGCCTTTATATCACCACAT

ATATGGTGGGGTAATTGAAAAGGNGGCCAATATACCGGTGGTNCTCCGGG

GAGGTCACACCAAGCAGTTAATATTTGGGCCACGGGGCACGCCATTTTTC

AACACTTTTTGAAACCCCGTTTTATTCCCCACCNGGGAAAAATGTCGTTT

CGNCACCTTTCTCCCTGGGTATACCCCCNAGGTGTTTTTNCCCCCGGGGG

GGCGNGTTTTTAAGGAGTATATATCATNNTTNCACCGGGTTTCCCCNCGC

CGACCANCCCNTTTGTCCCCATCACGGTTTTTTTNGGTTCTTTTTGGGCC

CCNCTTTAAAGAATACCTTTTTTAATGAGGGCCCCTTTGGNGGTTTATTC

ACCGAATAATNGGCACANAATTTTTTGGTCGGCGCACCGCATNATCNCCC

CACANNTGGTTGTAAACGGGCCCAGGATTTTTTTAACCAACTCTCCGGGG

GTTGGTCGACCCCACAAT

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cρ312_MDl/Ql_Kubota_Hp35

_01_A21_321.F.esd 537 MegaBACE

TGAGCTCAAGCAGTGGTACAACGCANTAGTACGCCCGCGTGGTCGCGGCC

GAGGTACGACTTCGGAGAAAGGAAGCCCACCGTGGGACAAGTACCTCAAC

TGTTACCCTATTGCCATCGACCTGATTTCCAAGGGCGTGGTCGACGTCAA

GCCCCTCATCACCCACCGCTTCAAGTTCGACGAATTCCAGAAAGCCTTTG

AGTTCTTCCGCAACGGGACAGACGGCGCCATCAAGTGCATAATTTCTTGT

GACTGAGTTTGACGTTGGGGAAAAGGGATGCTTACAGGTGGCTTTGCGTG

CATGTGCCTGCGAGCGTGTCTGTGTGTGTGGGAGAGAGAGAGAGAGAGAT

AAAGAATGTGTGTGAGAGAGAGAAAGAGAGCGAGAGCGAAAGAGAGAGAG

AGATAAAGAATGTCTGTGTGTGTGAGAGAGAACGAGAGCGAAAGAGAGAG

AAAGAGAGAGAGAGAGAGAGACAGAAGGATGACAGCGTGGCTGAATGAGA

GAGGAGACGAGAGCGAATATGGATGAATTAACACAAT

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Ql_Kubota_Hp35

_01_A23_353JF.esd 996 MegaBACE GATTGAGCNAAGCAGTGGTACAACGCAGAGTATCGCCCTCGAGCGGCCGC

CAGGTCAGGTACTTTTTGTANTTCGGTCTCTATTTTGAAATAACGTGCAT

AAATAGATTTATTAACACACACTCTCTTTTTATCTTTTGTGTGCTATATA

TTTAGATCATACATACGAAATATATCATATATAGAAAGTATAACATTNCA

GTTCAAAGTATCAANANATCATTATTGGATTCGTGAGCGTGAAGCATATG

AGGGTGAAGATATATATGCAGAAATGAATGANCGGTGACNTGAGTATGAT

GATGGCGATGGTGATGAAGCACAATAGCACGAGTCATCACAGTAGTCACA

GAGGCTTGCNATTGCCGGCTTGANNGGCTCCAGCAGCGGGCTATGCCCCA

AGCAGGAGCNGTCTACAGGCCGACTTATGNGAAACTCCCAATTCGCCCCC

TTGTAACACGGCGNCCGGGGCAGGTTCCCGGGCACGGGCCCCATTGGGGC

CTTTGGAAAAGGGGNAACCCTCAGGGTACGGGTTTTAACCCCTTTCCCGG

TGGCACCCGGACTGTGAACCAAAAGGCCTTGTTGGGGACCGGTTAACCGT

TTCATGGTTCCCGAGTACCGTNTGGAAAGCCGGTTTACCTTCGGCATTGT

CGTTAACCCGCCAAAGCGCCCTATCTAGTGTGGGCTTNNTACCCCCCCGT

TTANTTTTCAACNGGCTNGGGACAACANGGTGGGGCGGTTTTNTCCCAAA

AAAANTACTCTTTTATATTCGAGGGGATGCTCTGTGGGGCCCCACGGCGA

CCCCTTATATGGGCNCGGNGCCGAGGTTTTTAAAAAGAGTTTTCCCNCGA

AGATTGTTTTGGGAGGTGTTCCCCCCCTTTTATAANGGCCTTGGGTTGTG

TGTTTCCCCCNCTTTGGGGTATGAGAGTTTGNGAAACACAAGTTTTTCCG

GATTGTGTTTAAANTTCCNCGCCGGGCCTTTTTCCAACCCAAGACT

>E:\AnalyzedData/50425_4_2_Q l_Kubota_Hp35_01 RunO l_Cp312_MD 1/Q l_Kubota_Hp35

_01_Cl l_163.F.esd 1362 MegaBACE

AATGAGCTCCAAAGCAGTTGAGTACAACGCAGATGTACGCCCATCGCATG

CGGTCCGGNGAGCTGACNGGTGATACAGGTAATCTCATCGATNTACTGTG

TCTAAGTTTGGCTTACGTCAGTCACTCTGTACCTCGAAGTGACGCGCACA

ACCNCANACAGGTCGGAGAATTTCGAAGTGACACTCATATGGTCTAAGCA

CNATTTGTGCGTATGAATATCGTAAGCCCTCGTTTGTGAGGCATGAGCCG

TGCTGTTCCCCCCGTTGTCTTGATAGCTGACTGAAATCGAGGTGCATCAT

TACACTACAATAGGCCATCTTGAACTTCTATAGACAAAAACAGGATACCA

TATATAATNGACTTGGGAAGCTATCCCTGCGGTACCGGCCAGAAAGGCAA

TCTTGACGTAGTGGAGACTGTTGAGCATATTCATAGTTTCCGTTCTCATT

TTGGTATGACTGATTAAGNTTAGTAATAGGGTTATACACACATTGGGTTT

CTTTGGTTTTGGCCTTTTTTGGAGGCATTGAAAGGGTGATCTAAAAATAT

AGGAAGGACCAGGGACCATATAGGAGACAGATAAAAATACAAATTATGAG

NATTGCTAACTCACTTAGGTTGCGTATTGGGGCCTNAGGTAGCTAGGANA

CAGANTGTGGTGTCAGTTTACCCTNTTTTATCANNTCACAAGTTCTCTCT

AAAACAGGCCAAGATTAACACTTAGGACTAGGGGTCAAGCGCAGCAATGC

ATTTATAAGAGTGTTTGTCACTTATTTGACGCTTAGAGAGGGTGTGGTCA

CATATANTGGAGGCTGAGAATATGGCTGACATGTTCTAACCACCCNATTT

GCAGATTTCNAATGGACCGGTTTCTTGAGGTCTGTCCTCCCCTAAGGTTT

GGTCCGCAGCAANTTTTTTTGGTCCCCAAATNGTCCCCCGGGGNTGAACC

AACAACCACCACTTNGGTTCCCANGTCAAGCCACAAAGGCAACTTTAAGC

CAATAATGCAACCACCTGGGAGAAAATGCCCCANCTNTAACACAAAAGGT

TGGTCAGCAACAANGGGGGCGCCTAATNGGAAAAGGGGGTTTTGGGNGCT

CTTNAAGAGGATTTGATAAAAGTTTATGGAAGGGGCTTCANGAAGCCTTC

TTCATCATAACCCAACTTGGTTGANAATTGATCTTCCTGGCCAGAGTTAT

TAAGGAAAGGCTGTTTGTAACAAATTTTTNTGGGGCTAATCGTCGGAGGG

ATTCTTTNCGGANGAATAGAGCATTTGTNTAGTCGGGGAAAGAACTAAAG

GGNATGAGGGTTCCNNATTTGGGGGCGGNTTAAATTTTATNGGGTATTTT TTCNAAACTGTT

>E:\AnalyzedDatay50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Ql_Kubota_Hp35

_01_E05_069.F.esd 1264 MegaBACE

GGTNAGANATCGGACAGCAGTGTGTTAGNCAATCGCATCAGTACGCCGNT

CGTAGCCGGTCGGCTCAGTGGTCAGTGGCTACATCAGTCTCAAGCTACTT

CACATGAATATGTCACTAGTGTACATTGATTGACTTTATACTTGCATGGT

ACTCTGAGTCGTATTAAGACATCACAAAGATCATGACACGTTACCACGCG

TGCAGGTAGGCATGAAGTGCGATTAACAATACCGACGTCTCTAGTGTACG

TCTTCACCCATTATGCTGCCCTCATATGATGTAGGATGCTNCTGTTTATT

ACACAGCCGTCTATACAAATGGGCAACACTTCTTCTTATTACCACCCTGT

TCCATTTCACACTTTCTGTCGGGGGATTTATTGCCGGCGCTTTTAACTAC

TCGACCCAGTGCACCTCAATGTTCCANTCAGAAAGTAATCCTGCTTAATA

TTGGAACCATTATAGGAATCGTTCTCAAGCTNTCNTTGGCTTTGGAACTT

CATTTCCTAAGGGAAAAGTGGCCGGCCNAAAGCACGGAAATTGAACCTTT

TTGGGGCCCAAGGGGTCTGGAAGNGTTCCCCGTTGGGGTTCCGTTTCCCC

ANAAACCGAGAACGACCGTGGCTCAGTTCCTTACNCAAAGTTNCAACCAA

CGTCCTGGGNTCTNAACCCCCAACCGTAATGGGAATCCCATTGTCGCGAG

GGAACCGGGTTACCAATACCGTCGGGTCGTTCGCCCTTTTTCGGCCAGAC

TTTTCCCTTGGGACAAAGGTAAAACATTCCCCTTCTGGGGGTCTTTCCTC

AACCCGGGTTTTTAAAGCGAAATTTCCGTTCTNNGGGTNTCAAGANTAAA

TTCCCGAACTTCTTNTGGCGGACCAATCCTGGGTTCAACACCCCCCCGTT

ATCCTGCGGGGACACGCCCCGGGTCGCGGAAAAAAACCCAAAACTCTGGG

GCCCCTGATGTGTGGGGGGGGGGCCCGNGTGCTTNCAACACCCTNTTTTT

TTTCCCCATTTTGGGGCGTTTTCCCCGNGTGTTTCCCCCTTTTGTTTGGC

GCAANAGTGGCCCCCCGGGGTTTTAAAGCCGCNTTGTGGGTAAATTTATG

GCCGGCGTTTAGAACCCCCCCCCCCCAATGTGTGCCCCGTTNTATGATGA

TCTCTGCTCGGGGCTTTATTCCCCCCCCCNTTANTATTCCTCTACAGGGG

TCTAGGCANAAGGCAGGCGGGTTTGTCANAGAGCAGTTTTTGGGGCCTTG

GGCCCCGNGCCCCT

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Ql_Kubota_Hp35

_01_E19_293.F.esd 525 MegaBACE

GGAGCTCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGCCG

AGGTACCTTGGTTTGTTGCATTACCTTTGTGTCGCTATTACTCTAATGAA

ACTAGACCATGCGAGACCCATAGATGCAGCCAAGAGACACAACCAAGCAA

GGACTAAACTCAGACCTTTGCTCCATGTCCCATGACACCCTACAACTATG

TAACAATACAGATTAACTGGTGAAGAAAAGACATTCCCTTGCACCGTAAT

GAACAACAAATGACAACAGAAATAAATTAGGGATGTGGTCATGTAATACC

GTTTAACTGTCATTCACTACTATTCAACTATGGAAGGTTGCCTATTGTTG

CCTTGCCAGTAGTCCAGGAGTCAAGGGGGCTACCTCCTCACCGTCTCGGT

TTCTGTGAATGATGATGCCTTCCATCCAGTGTCTGAAACTCTCCATCTAG

ACTCCTCAGAATTGTTTTTAGTATTATCTATGTATATATTATCTCCATGG

GTGGCCAACTCCATGACAGCAACCA

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Ql_Kubota_Hp35

J)l_G21_327.F.esd 608 MegaBACE

ATGAGCCAAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCGCCAGG

CAGGTACTCGGCTTCTCCCTCTTCGTCTCCGGGTTTATCCTGACGGTGCT

GGCCTACGCGCCCACGGACTCCTACCTGATGGTCATGATTCAGAGCATGG

GCGTGATGGGTTTCCTTGGCCCAATTGTGCTCGTCATTGGCACGATCATG

CTCCTGGTGTCCATCTTCTACTGCTTATTCGGCAACACTGAAGACGACGA TTACTAGCCGAACGTTCGATCCTCTGACGTTATAAGCCGCCATTTCCAGC

CTGCAAACAATGAGAACAAAAGACTTTTTTCCAGGAATTAAACAATCGTT

GGTCGCGATGTTGACTCACATCTTTTATCCTTTTTTTTCTGTGCCAAATG

CAGAAAAGATATGAATGAGAATCAATACTTTCGCAGCAGAAGAGATGTAT

TTGACGAGTTTCGGAGACATCTTCGTCAGAAATGCATGTATTTCTGAAGA

TATAATCGAAAACCGGGTTAAATATAGCTCTTGATATTGTGAATATATTA

ATTATCTTTCATACCTTTCCTAACATTTTTGCTTTATTTTTTTCCCTTCT

TTTTTTCA

>E:\AnalyzedData/50425_4_2_Q l_Kubota_Hp35_01 RunO l_Cp312_MD 1 /Q l_Kubota_Hp35

_01_Il l_169.F.esd 521 MegaBACE

TGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAGGC

AGGTACCATTTTGCTGACCAACCAGGAGAACGCTCTATTGAACCCAGCTA

TGAAGAGGTCAAAAACATTCTAACAGGGGTTGGATTTAAAATTACCGTTG

AAGAAACTGGGATGCATACAGCTTACACCCAAAATGTTCGGTCGATGCTG

AAGTATGAATATGATAGTGTCTTCTTTGTTGCCATGAAACCTTAACACTG

GCACTGTAGCAAAAGATGAAACAGTATCAGTTATCTTGACATAATTTCAA

AGATGATAGGATAAATGCATCTTGATATTTTCTCCACTTTTGATATATAT

TGACATACATAAAAAAAAACATTGATATGAAGAGGATGGCAAGCAAAACT

TTTTATCTTAATGTGATACGGAAATTTTAAAGTAAATTTGCTACTGCCTA

GGTTATTATAAAGGTAGCTTAGATAATCAATATCTGATAATGATGAAGTA

AAGAGGTAATATACCTTACTA

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Ql_Kubota_Hp35

_01J23_361.F.esd 480 MegaBACE

GATGAGCCAAGCAGTGGTACACGCAGTAGTACGCCCTCGAGCGGCGCAGG

CAGGTACCAATAAATCTAATTTTTAAGGAAACCTTTAAAAGTTTTTTCAA

TGATTCCTTGCGACCATAATCATATCACCACTGAAAAATACCGAACAATC

ACCAAGAGCTCTGATTAAAAAGCGGAAGCAAAAATAAGGAGAGCAGTGCA

GCGACAGGTATCCAGGTAAGACCCAATACTCACGTGAGGTGACCTTCTAG

TTGTCACTTGCTTCAGTATCCTTGGCCTGATCCGTCTGTAGTTCACTGTA

GGACCTAGACAATGCCCTGGGCCAGATGCTGTTCGACCTACTACCACCCC

CACTGTTCACAAGCATCGTAAGCGACCAGAACTTGATGACGGCGATGTGA

CCGCGTGCTAGAGCGCACCGAGGCAGCGCCGGCCGGCAACATCTCTTGCA

GGTGGAGCGTGTGAGAGCTTGAGCGAGATG

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cρ312_MDl/Ql_Kubota_Hp35

_01JC09_139.F.esd 470 MegaBACE

TGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAGGC

AGGTACGCGGGTGCCATGCATTCATTAATCTGATAAAAGCGTTATAATTA

GGTGCTTGATTTGCCGTATATATTATCATGGACTCATAAGGTTGATGTTA

GTGCACAATATCCTCATAATGTCTTITGCTGATTTTCATTTGCGCTTTGG

TTTATTTATTTAGTCTTTATCTAACACCAGATAACCTATCCAAACTCACA

TGTTTATTCCCAGTGTCTTTACAAAGAAGTGATGTTAAACGCAGTGAGAT

TCTTTTTCGAATATTTGGATTGTTATTTTAGTTAAAAGCATTTGATCAAC

TTAAAGGACTTGGACACCGACTGCAATTGATGCATTCTGTTCGTACTCTT

CTTATGGCATTCTATCAGTGCCTAAGCTAGCTCTGTTGAAGAGCATATTT

ATTTTAATGAGAATATCAAT

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Rxin01_Cp312_MDl/Ql_Kubota_Hp35

_01_K17_267.F.esd 655 MegaBACE

TGAGCCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAGG

CAGGTACCATCATTTTAAGCATAAACTTAAGGCTAAGAATAAATCGAAGG ACAGAATCTCAATACTGACTCAAAGCAAAATCAAATAAATTTCTTTATTC

TTAGCAGTTCAGGTGGCAGTGGGCTGTGTCAGCCGTGAATCAAAAAGGTC

TCGAGTATCATAGGCCAATGAATGTGATTGCCAATAAGTGTTCCTTCTGC

ATAAGGAACATTAATGACTAATATGACTATGAAAACAGGTGTGATAAGTG

GCATTCCTTGTTAAGGAATGTCATTTATTACTCAATCAGAAAAATAAAGA

TCATTTACTATTAATATTCTGTTTGGCTCTTCACCAGTGGCTCACAGAAC

ATCATAAAGTAATGGCTTCGAGAAATAGAATTCACTATGTTAACGACATA

AAATTTATATATAACCTCCTTTGACCACGAGAATCCCCCAAGAATACCCA

GAGCGACTTAAGGATTCGAAATGAATCAGAGGGCATCGCTGACCTGATTC

CTCTACAAGGAGACAGAGCTTTCCTTCAGAATTCTTCCGGAAACCAGCAG

CCTCTTCAAGTCGGTNCTTCAGTCTCAGGAGGGAAGGTCGACCACATCCG

GCCCG

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01 RunO l_Cp312_MD l/Ql_Kubota_Hp35

_01_M01_013.F.esd 757 MegaBACE

GCTGACGCCACAGCGTGCGTACAGGCACGAAGCTNAAACAATCCATACGG

AGCGTGTGGGCGCTCGAAGTCAGGGCCGAGATNGCATGTGATTGTTGCCN

ATCCTAATCGATTGGTGCACTTCNAATATTATGTGATNTGACNTGAACTT

NCTTACTCACGTGCNCGTCTGGGTAGTATCCCACNCGGGACGGGGATACT

TATGTGCCCGTTCACAATTACTTGTCCTGGTTGTCCGGTCGATGGGCGTA

CCTTCTGTCCGTCTAGAGNCGTTNTNGCTGGTCCCCCAGNCTTTGGTCCC

CNTTACGTGATGGGTTATTTGCNCCGCTTTGGNCGTAGTCCTGGNCAGTA

AGGGTGNCTCCGTGTGTGACCNTGTTATCCCGGGTCGACAATTTCCACAG

CAAGGATACTAGGCCGGGACCTACTGTGTCCTGGCTTGGGGTGCCTAATT

GAGNTGCAGCTAACTTCCACCATTAACATTATGGCGTATTCGNGGCTACC

ACTTGGCCCCGGTGNTTACCCGACAGTTCGGGGGGGCAAACCCTTTAGTT

CCGNTTGGACCCAAGCTTAGCATTTCNCNACTTGACAANTTCGTGGCCAA

AACGCCTGCCGGGTGGGAGCGAAGGGGCGGGGATCCATAGGGTGTCATTT

AGGGGGCGGCCTCCTTTCACCNGCTTTCCCNCCGGGTTACACACATGTGA

GAACTTCGCTAGGGNCCATCCCGGGTCGGNCACCCGGGGCTATGNTTGAT

GGGGGAC

>E:\AnalyzedData/50425_4_2_Q 1 __Kubota_Hp35_0 lRunO l_Cp312_MD 1 /Q 1 _Kubota_Hp35

_01_M15_237.F.esd 1271 MegaBACE

TGAGCCAAAGCAGTGGTACACGCAGAGTACGCCCTCGGATGCGGCCGGCG

AGCGACGAGTGATACGTAGTGNCCGATTAAGGTTGATCCTTACATCGGTA

AATGTNTGATTTAACCACACTGGATGATCGACTTGCGAAACAACATGTTC

GTAAAACATAGCACTAGCACACGATGAATCCACAGTCATTTCTTTCACAG

GCTATTCACTAATAGTATATTGTGATATACATCTATTACATTAGTAACAT

CTGGAGGTACACGTACTTATGCGCGCACAATAGCACCCATAGGTCCGAAA

TCTTAGTGAAGTATCTCCTGGACGTGTCTAATCTACACAGCTATCCGTAN

NTNAGTTAAACNGTGAAGAACCTTAACTATTAGCATACAGGAATTTTCCT

CTGAAAATTACCCCCGATTCTTTTCATNCCNACTCGGCCAATGTTTTGCA

CTCGATTCCGACTTCGNATACATGTGAAAATTCNCCCAAGGAAAATAGTT

CGAGGCAAAAATTCCAANGTCGGTCAATTCNCNGTTGTTTGGCCCATTTC

GGATAGTTGTGAAAGAAACCTAAACTAAAAAGGAATAATTATACAGATTT

AATTTAAANGAGCAATTCCTAGCAATGGGTAACAGGAAGGAAAAATTTAG

GGAATGGGTTTGGGTCATTGGAAAGGAAGGCAAGTTTATAGGGCGGTTNG

AACCCAACGTTGGTGCCGGTGGAATTCGGTTCTTTTTCCGGGCAACCGGT

NGGGGGGCCCGCCTTNAACCCCAATTTTAACCAATTNACAGNGTAAACCC

GATAAACCCTAAAAATAGGCCAAAGCGGCCACAGAATTTACCCCGTTAAC GGGTCATTCTTCGTAAGTTCCAATTTTNCTCCTAAAGOGAAACCCCCTTG

AACATAATTGGGGTTTCACCCGCGCCCCCTTATGGTTTGTTACAACACAC

TTCTGGCCGCCCCAGAATTGGTCGCCCTTGAAAAAGAGGGTTTTTTTGTG

GGGGGCACATNTTCCANAAGAAAGCGGTTAATATTGAGGCCTGGTACGCG

CGGGTGGGATTTTAAAACTACCACAATCTTTNTTCTGTTGGGGTAAAACA

ACACGCTGTGTGGANACGAGTGTTGTCAGGGTTGTGGGAGAGAAGAAAAT

ATAAAAACACCCTTTTTGAGAAGACTTCTGAGAGATATAATTGTGTGCTA

TAGAGAGGAAAAAAGGTNTCCCCGGGGGGGCTCCCTAAAAAAAGAAATGT

NNGTTTTACACACCGGCCGGG

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Ql_Kubota_Hp35

_01_O07_l l l .F.esd 738 MegaBACE

GNTGAGCTCAAGCAGTGGTACACGCANTAGTACGCCCAGCGTGGTCGCGG

CCGAGGTACGCCGCTGCCGGAGGTTCAGACGACTGGGCCAAGGGAGAAGG

TGGAGTCAAATTCGCCTACACCGTAGAACTTCGTGACACTGGCAACTACG

GCTTCGTCCTCCCCCCTGACCAGATTATCCCCACCGGAGAGGAGACCTTC

GAGGCTCTCAAGGTGGTCGCTAACTACGTCAAGGACAACTACCCGATTTA

AAGGTGTGGTGTTGAAAAGGATTGGAATGGACCAAGCGTCTTGAGAAAAC

GGCGAGATGTGATTCGGAGAAGGAAATTACACCACTTTGGCANCGNACTT

TTNTGGCATGNCGGCNNCGCNACACACAAAATCAACAACAAACTANACAC

NCCGCNACACACCAAACATCACANCACTACATANNNACAATCACCNAANC

ACNATAATACTAAACAACAAGAAGGCTTTAAAACCGCNTTTGGTGCCCTG

CCTTGTTGGTGGCGCTCGGTGGGACCACCGGGGGCCTTTTCCCGGAAACG

GCAGGGCCCCCGATTAAACCTTTGTCCCTTTAGGTTCCCCGGGTTTCACT

TTTGTTTGGACAAAGAGCCCCGGGTTTTAAACCCCTTTTGGGAAACTTTG

GCTGGTTAAAACCCCCCAAAGGTCACCACTTTTTCACTTCAGTGCGGGAT

TTITTCCCACCCGCCCCTTTTTTTCTTTAAGAGATTTG

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01 RunO l_Cp312_MD1 /Q l_Kubota_Hp35

_01_O23_367.F.esd 1432 MegaBACE

GGAATTGGAGCTCTATAGCAGTNTGTACANCGCAGAGTANNGCCCTCGTA

GCTGGCCGCCATGGCCAGGTACGCGGAAAGCGAGAAGTGAGAGAGAGGAA

GAGAAGAAGAGAGTGAGAAGAAGGAAGGAAGAGAAGCGGGAGGAAGGACA

AGGAGAGAAGAAGGGAAGAAAGAGGAATGGTGATGCGAAAGAGGACACAT

GGTGTACAGAAGCAAAAACATTGGAGGGAAAGAAGGACAAGGTGTAAGGA

GAAAAAATCATTGGACGGGGANAAGGAGGGAAAAAGGGTGTAAGGAAGAA

CAAAAAATGGAGGGGAACAAAGGAAGAATTGAAAAGGGGGGNAAGGGGGG

GGAAAAACAACGGCAAGGCAAGGGGAAAAACAGGGGGGAACGGTGGGAAA

CCAGGAACNGCAAGGAAAAAACANTAAGCANAGCAAAGAAACAAAAATAA

AACAANAGANAGAATCACAGAAGAAGAGTGTTAACAGGCCAGGNGGGTTT

AAAAAGGACCAAGTTGGGTTAAACCAAAACGGCAAAGAGTCAAGGGTTAA

CCACCCCTTTCCTGGTGACCACCCAGATGGAAANCACAAAACNAGGGCCT

TTGTGCGGCGACTCGTGTTTTAACAGCATTTTTACACATTTTGGGTGTTT

TCCCACAGAGAATTTCCACCAATTTTGGAGACGAAAGGGACCCCANGGGT

GTTTTCACAACCCTTGTTTGAGAGACACATTCGGACGNGGAGTTTTTAAA

ACCACCCCACCCGAAAAAATGAGGCCACNCTTTTTATTAAACGGCTACGG

GAATTAATNTCCACACNCCCCCATATTTAAATGTTAATTACAAACGAGAT

GTTTTGATGGTAAAACAGAGACGCGCAGGGCNTTTTCNTACAAAAACAGC

AGGTTTATATTGAGGGGACCACTAGAGCGACCCCAGTAGCAGACACCCAT

CTTTTTNTGAGAAGCAGCGACCGCTGTCGAGTTTTTACAGTGAAGTGTCT

TCCCCACACACTTTTTTCGGGGAGGAANTTCTCCCCGAAGATAGATTTAC CAANGCGAGAGCGCACCTTTTATGGGGGACAGTCCATTATCTNATATAGC

ACCCAGCCCCATATTTTTTAGGGGAAGCACTTTCTGCGAGGGGCATTTTT

TTTCGAGGCGGAAGACAACACAAGCAGCCAACNAGNAGATACACGACTTT

TATTCATTGCATGGGAAGGGAGCACATTCTACAATTACANACAAAGGAAT

TCGTTTTTACCNCCACACCACACCAGCCTAGGTATGTAGAAGCCACCTAA

TTTTTTTTCCCGCCCCGAAGAAAGCANNCCCGCATACATAGCAAAANAAA

CTNTTTTTCTANTTCGACACCAGCACCCCCTAANAATGGACNACCATGAA

AAAACTGCCATCCACAAGAAATAANTANCTAC

>E:\AnalyzedData/50425_4_2_Ql_Kubota_H_P35_01Run01_Cp312_MDl/Q2_Kubota_Hp35

__01_A04_049.F.esd 754 MegaBACE

TGGAGTCAAGCAGTGGTAACACGCAGTAGATACGCCCCGTTGGTCGCGGC

GAGGTACCAGAATGTGACATTGAACGGCTCCAATTGGAGTTGTGATGTAA

CTATNACCTCAAGGGTTTGGAGGAATATTTTGTCTGGAGAGTACGCGGGA

AAAAAATGGGGTTGGNCAAGGTTAAAGTCTCCGGTTCTCAAATCTTATTG

TTGGGAAAACTTNGGATTGTCCTGGGTGTGGGAAAAAATGTTTTGGTAAA

CCGGGGGATTGAAGAACATTTGTTGNTGGTGTGACGGAGTTGTGAAAGCG

CTTTTCTTCATGTTTGGGGNCCCCCGTTAAACAAGGTTGATTGNCGCCCA

CGATATTTGGAGCGAAAGGCTCAAAGGATTAGTTGAGTACGCGACTGGGC

TTGTAGAAGACAAGGTGGANTACATCACTTATATGGGGACCGTCGATTTT

CTACAGAAAAGTATNAGAAGACCTTCCCAATAGTTTGGCCAGAAGAAAAA

CTTGGTCCTTTTGTGGGGGGCGTTGGGGGTGTTTTTACCGACCAGGGNAT

ATATCTTGGGACATTAACAAGTTTTCAATGGGGGATTAGTCAATACTTNC

CTCTTAAATAAAAGGTCTTTAACTAACNTGTTGGCTGGGATTTCAATAGA

CATAGGCTTGGGTGTTGTATTAATTAGCGATTNGCATTTTGCTTGGGAGG

GNAGTAGGAGGTTCATTAGTTCAGAACAGGTAACCTTGCCTTGANGNGGG

CGGT

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q2_Kubota_Hp35

__01_C06_083.F.esd 736 MegaBACE

GTGAGCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGCCG

AGGTACCAAATCATAAGCTTGGCTAGATCTCTGGAGTAGATAAACTGCCG

ACGAGGATTTCCAGTTCCCCATACAGTAAATGGTGTCCCATTTTTCTTGG

CCGTGTAAATCTTGTTTATTAGTCCTGGCAACACATGGCCATCCTCCAAG

CTGAAGTTGTCATATGGTCCATAGACATTTGTTGGAATAACTGAAGTGAA

ATTACAGCCATATTGTAGATGATATACTTGGTTCATTACATCAATCATCC

TCTTAGCATGACTATACCCAAAGTTAGAGTGATGTGGAGGGCCATTATGG

ATCATTGTTTCATCAATAGGGTATGTAGTTTTGTCTAGAAAGATGCAAGT

TGAAAGACAAGAAACTACTTTCTTCACTTTGAATTCCTTGCATAAATCCA

ATACATTGTCATTGATTGCCATATTCAATCGAAGGAAATCGCAGTTGTAT

TTCATGTTTCGGTAAAGGCCTCCCACCAATGCAGCCAGATGAATAACATG

AGTTGGCTTATATTTCTCAAACAAAGCTCGGGTTTCAGCTTTGTTTTTCA

GATCACCATCCTTTGAGCTGATAAATATCCAGCGCTCATTTGGGCGTTTC

TCCTCTTCTGCAACAGCTCTTATTGCTTGTCCTACCAATCCCGTGCCTCC

AGTCACCAGAATCACCATCTCTGTTTCAGCCATTTT

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q2_Kubota_Hp35

01_E10_149.F.esd 497 MegaBACE

TGAGCNCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAGG CAGGTACATGGGATTATAGTTCAAAATAGCTTCCTATTAATGCTGGATAT ACTCGCCATGGTTGAAGACCTTAACTTGGATGTGGCCGAAGTTGGGAAGA TCTTCGAATACACGTTCATCGGGAACCTTGCGATCCAGAGGGTAGCCATG TGGGCGATTATCAGGGTATTTACCATGAGCACCATAGTGATTGAATTCGG

TATTTTCGTGGAGGCCATCCAATGCTGCGTCGGCTGCGCCATCAGTCACG

GCAACAACAAGGTCGAACTCCAGACCCTGTTCATTACCCTTGGGGAGCAG

GAATCGATTTGGAATACCAGTGGCACTCTCGAAATCTGCAAGTCCGGAGT

CTGCACCACCTAAGGCTTCTTTGGTCTTCTCAAAGAGTGTTGCAAAACTA

GGCACATCAGGCACAGTAACAGCAGATTCCGAGCATTTACGCTCGAT

>E:\AnalyzedData/50425_4_2_Q l_Kubota_Hp35_01 RunO l_Cp312_MD 1 /Q2_Kubota_Hp35

__01_E24_373.F.esd 1562 . MegaBACE

GNGTTGGACGCTCGACAGCAGTNTTGTCACCTACAATCCGCAAGACGTTA

ACAGCCACGATAATAACAGACNTCACGTAGTGATGNAGCAATGCGTGTTG

CGATCTGCAGCGACCAGAGTGCTACCTGTANATGTACACGCCGCCATATT

AGTCAATCCATGAAAGCATATTGCNNNATCCGTGGGGCTTCTGTGTATTA

CGAACTTCCCAGTATCTTTTGCGAAACCCCAACAGACACATAGCTAACAT

TAGTTCTAGTCAANTCACATCTGTTTGTAGGCTTTGGAGGCGCACGCTAT

AATCGAACATGCTCGCTATCTTGGGAACTCGACAAAAGAGTCCTGATACT

CTTGTGTTGGGCAGATNCGCATCGTAGCTTGGGGCCTCTTTAGGGCGGGA

CCCGGACAGCGGAACCCACTGAATCGTGAACCTGGGAAAAAAGTGCTTCT

CCGCAACTTTTCATAGCCTTGAGCGACGTAGGGAGCACAATCCGGTACCC

TTGCCGAACACCGTATCCCGTACTCGCGTTANACTCAAGAGAGAAGAGTG

TAACCGGCAATNAGTAAGGGGGCGCACGAGAAAGGCCACCGTAACCCCAA

TAAGATNTATCTTTAAACTAGGGTTGCCATAATTATCGGTACCCCTCCTA

ATATAGGCCATTTCTTTTTATTACGNGGNGGGCTAAAAAAAAAGGAAGGG

TTTTTGTGGGGNCCAAGGTTACCGCCGTTTTATTGGGGGAACTTTAAATT

TANTCAAATTTGTCCTCTATATCTTGGTAAAACNTTTCGGGAGAAATTAA

AGATTTTATGTGGGGTTTATTAATNTACCTGTTGGGNATATTGAGGGAAC

ATTATATAAAAGTTNAGGGTTAATTAATGCGCTTTATTAAAATTACCGAC

AGGAANGAAAAACTNTNATGNT AAAGAAGGACAGCNGTTACeCTTATTTA

NCTNTAAGNAGGGCGCCCTAAAACTATATGGGCCGCTANATTTAACCCTT

TTCACCCTGGTGTGGGGGAAAATTAACAAATAAACNGNGGGCCGTAATTT

TGTGGTGTTTCACCCCTAATCGAGAAACTTTGAAGAAAACTACTAAAACG

ACATACAGCGGTATATANCAGAANACCTACATGATCAAAAGCATATCAAC

CCAACGACAGAAACAAANATGCTACTAAGAAATTGGATAAAAAACCCGCC

TTTTGGGTCCCCCCCCCTAATGTGGTGGGCNCTGNGTTGGGTCCTCCGCC

GCACNATTACCCTGTAGCACCACGGNGGGTCGGGGCCCTTGGTATTNAAG

GCCTTTATTTTACGTTTTGTAGGGCGTCACCGCGGGTTCCCCTTTACTGG

GCAGCAAGGGCCCCTGGTTTATACACCCCTNTTGGGCACACTCTTGGGTG

GTGTTAAACCCCCCGCCACAAAAGAGGGCTTTTANTACTTCTCTGTGGGT

TTACTTCCCCCCCCCACCNAGTTTTTGTAANTGAAGAGGTCTNCGGGCAN

CGGGTGTGGCNNGCGTCTAATTACACGAGCAGCTCCTNTGNGTCNACCGC

CCTCTGTGCCCG

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q2_Kubota_Hp35

_01_I02_025.F.esd 586 MegaBACE

GATGAGCTCAAGCAGTGGTACACGCAGTAGTACGCCCTCGAGCGGCCGCC

AGGCAGGTACGCGGGAGTTGAGCATTGGACTTCAACGAGAAGTTAGCCTT

CGAACCAGAATCACCACCACCCACCACCACCATCATGAGCGCCGAAACTC

CCCTCAAGGACCTTCCCAAGGTTGACCCCACCCTCAAGGGACAGCTCGAG

GGATTCTCCGCCGTAAACCTTAAGAAGACCGAGACGGAGGAAAAGTGCCA

CCTGCCAAACAAGGAGGACGTGGCACAAGAGAAGCAACACATAGAACATC

TACAGAACATCAGCGAGTTTCGCAGCGAAAGACTCAAACGAACGCCCACC TCTGAGAAGTTGGTCCTCCCCAGTAGTCAAGACGTGGAAGCAGAGAAGGC

AGCACAGGCCCATCTGCAGGCCGTCGAAGGCTTCAATACTGCACAACTCA

AGCATGCCAATATCCAAGAAAAAATTGTTTTACCTGCTAAGGAAGATATC

GAGACTGAGAAGACACATCAGAGCATTTTCCAAGGTGTAACAGGATTTGA

CAAGTCCCAAATGCGACATGCCGAGACCGAAGAAAA

>E:\AnalyzedData/50425_4_2_Q l_Kubota_Hp35_01 RunO l_Cp312 __MD 1 /Q2_Kubota_Hp35

_01_I06_089.F,esd 1624 MegaBACE

GTGGAGCTCCAAAGCAGTGGTAACAACGCAGAAGTACGCCCGTCGGACGC

TGGTCGTCGATGCGGTCATGTGATACCTGTTACTTGCATNGTNGTACCTC

CGTTTTTCTTAATTGTTTTCCTCTTTTCGAATTGCAGCGTGCTTAACATT

ATAGACACCAGACTTAAGTACTNTTACAATAAATTGAGTNTCTATTTAAG

TCTATGCTTTTCTNATGAGTGTTATATATATTGGCATCACTGTAATCTAT

ACATTTCACGACCAACGAGATTTATTAACTGTATGCGCCCGGCGGATACC

ACTTCGAGGCTNGTCGTACCACCTGCGTGGTGCGNGTACTGTCTGTGTAC

GGCTCATATGCATCACGATAACATAGAATGGGTACCCCACTNCTATCTTG

GCTCCGCCTTTTTCACATGTACGGGGGGTATAACTCTTGGCTTGCACGAG

TCGTCTCGAAGCCCGTTTGANCATTTCAAATAGTGGGTACATTTCAGGGC

CTTCGAATATCTNCCCCCTCGNTTGTTCGAAAAAAGNGTCGTNTTATTGC

GCCCGTAATCCAACGAGAGTTTCCCTACCTAGTCACATGCATTTACCCTT

ACGCCCCNTANCANGATGGCCCTTCCAGACTTTGGTTACATACCGGCGCT

TCCGGGTTGGGGCTTTGCCCTTGAAAAGTTGGCAAGGAATTGCCACGGGG

CCTAATTAACACTNCCCATCCAATTNCTTTACCACCTANNAGGCGGGCCC

TTGGGTACAGTTCTCATACCAAGCGTTTGTCACCTCCCGATCTTCATGTC

CACCAAGTGCCGTCCTCGNGGCGTAATCTCAAACCCCCTTTGATCCGGGG

ATAAGGNCCAANATCATTTTTGCTGATTGTAATAANACCATAGGAATAAN

TGGTGNGCCGACTCCCAATAGAGCGAGGACTGCGGCCGGCGAAGAGGGAA

AGAAACCGCCGGGCTGAGAGGAATTATAGGGCGGGCTTCANGTCTTTGGT

TGGCTGGCCGNTCCATTTCCCTTATTTACCCTGGGGGNTCATANACCTTT

ACGGGGNCCTTTTTCCGACACGGTTTGGAAACCTTCCGTTGCGAGATAGN

AGAAACCCTNACAGTTCTGCATCCCAGNTTATACCNAAGTTCTCGGTAGG

NCNGNCGTCTCCCGAAAAGGGGCCTGGCNGATTCAAATTACCGATTTGGG

GGTTCCCGCAACACNTCCCCACACCAAGGANGGGGCACGGGGGGCAAACA

GTTATAAATACNGAGGGTATNAATTNTACCCAACGAGGGGAAAAATTTTT

ACAAAGCGGGGGGTCGATGTAAAACAGGGCCATTCGGGGAAATTAGACGG

GGTAGNACCAGATTCGGTTTNGGAACCGTTCTGAATTAGATCTGGGCATC

CAACCGTTGAAAACGCGGGNTGGTCACAACGGGGAAAAATACAGGGTTAT

AAGAGNAAACACGNGGGCCCTTTGCGTTGTTTATCGGGCCTTTGTAGGCG

GGTGAAAACATTCCGCTAAGTTNGNGGCGTTTTCATCCGGTTCCACTCNC

CGGCTTTTGGAACGGGACGTTGGGNTATCAGAGAAAAATAACATATAACG

GGGAAACNGGGGTTCAATAAATGC

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q2_Kubota_Hp35

_01_K08_123.F.esd 507 MegaBACE

GANGAGCCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGC

CGAGGTACACTTAGGTCTCTGCATCTGTCTCTCAGACACCACAACAAGTG

TTACCTCAGGGACACTGTTCACTAGCTCGCTCACTGTCTGTTCCAACCAT

GGCCAACACTTCTCTCGTGATCATTATGATGCTTGATCGACGAGACTCAA

GTTGGGGCACACAGCTGTTTATCACATCCAATATATGTCATTCACTCATT

ATAGTTTTAAATACATACATCTAAGTTACAGTCCTCGTCCTCCTCTTTTT

TTTTTTTTTCTTCCCACTTTATTACATACATCTTAGGCATCTTGTTTTTT TTTTTTTTTTTTGTTTTTTCATAAAACTGATTTATGCTGAACCTGCCCAA

ACAAAAATAATATTCCTTACGCTCTTTTTCTCCCTTACACTTATACATCA

GTATAAGCAACAAACACTAAATTTTGAATAGATGGATTAAAACGTGGAAC

ATGCATT

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q2_Kubota_Hp35

_01_K14_219.F.esd 879 MegaBACE

TGGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAGGCA

GGTACGCGGGAAAGACTCTTAGCGGGGGATCACTTGGCTCGTGGGTCGAG

GAAGACCGCGGCAAGAAGCGAAGTTTTGGTGGCTATCGCAATTCTCCCGT

GATTCCACGACGTGTCGAACGCACATTGCGGCGGCCCTGTGCCTTCTTCG

AAGGACGGCTGCCACCTTCCCTCGAGTGTCGTGGTACTGAACCACTGAGC

TCAAGAGGTAATGAACGGCAGCAGCAACCACACCTTCTCTAATCAAGAAG

AGATGACCTTCATTGACTACCACAGCATTCTATGCGGTGAAGGACAACGG

CCACCGCAATATTTCAATGGAGCTGTAGTCTGCTACCGCTACCACAACGG

GAATCTAAGACGACAGATAACTGCCTAAATGCAATCAAAACAACGAAGAA

TAGCAGTCTTTGAAGGAAACGGTAACTAATCGGATTTTCTATATTTTCTT

ACACTAGATCGTTTGCAGAGCTGGTGAAATTTCAGCCCTTAAAAGGAAAC

GGTGACTTTTCTGATTTTCAATTTCTTACTTGCATAGCTTGATGTAATAA

CAGCATTCTTCCAAGGGAATTTAAAACCATACAGTCATTTTGTCCGTCTT

CCTCTTCGGAGCTATAAATAGCAAACATTTGAACATTGCTCAATTAACAA

TATGCGCACTATCGAAGATTGGAAATTTGCATTTTGTGAAAGTACTCGGC

GGGAACAACGCTGGGCGTANTCTGTCGTTTGAGCGTACTGATGTACCAAT

TTTGTTCCTTTATGAGGTTAATGGCGTTGGGNAAAAAGGCATAATGTTCC

TGGGTNATTTNCGNNTCTAATCNCACAAG

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q2_Kubota_Hρ35

_01_M10_157.F.esd 540 MegaBACE

GTGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAGG

CAGGTACATATACTTACAAAATATAAACCAACCCAAATTCATGTAAACTC

ACACAAGGCTCCAGGACTAAATAAATTCATCTCCCTCCTCATAAAATTTC

ATCAAGAATATTCCGTGTCATATATCATTATTATAAAGTTTATCTTTTTT

CCAAGGGCCAAATAAGAATACTTATTATTAGGTGTAATATTATCACTATT

ATTCTTAAGCTATTGAAATTTAACTCTTATATAATTTTGATGAATTATTG

TGGAAAAGATGTTTCAAAAGGTCCATCCTATAACCCAGGATCTTCATGAT

ACTCTGCATTTTCCCTCGGGACTGATGTTGCCGTCAGCCTTCTCGATCTC

CAGAATGACACGTGTGAAGATATCATTTACAGCCTCATGTTGTTTGGCAC

TTGTCTCCAGGAAAACAGCCTTCCACATCTCGGCAATTTTGCGTCCTTGA

TCTGTGGTCACAACACGTTCCATGTGAAGATCTGTTTTGT

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q2_Kubota_Hp35

_01_O16_255.F.esd 537 MegaBACE

TGAGCCAAGCAGTGTACACGCAGAGTAGCCGCGTGATCGCGCCGAGGTAC

TGGTCCATTGATTGNAATTAACTCAGCNGTTCCTANTAAGACTCCGATTT

GAGTATTAATGGGTACCCAGTGCGATNGTCTACTCCTAGAAAGTCGGAAT

CCGTCCTCAGGCAGTCTCATNGGAGGATTCTATAGGATTCAATTCCTANT

TGAAGAAGGTCTGATATACGTATCTTCATTATCATTGNTGACCAATAAGT

TTTTAATTNGGTTTAAACNTTNCCTTNAAGGGAATTTTAATTTAACTTAT

CCATCTAAGTAGGTATAAAAAGTCGGAGTGAAGGAAGAGCAATAAAAGAA

TAAGAATATAAAGNTGGTAAAACAGTTCATAACAATTTCAATAAGTATTG

TCCTTCTAAATAAAGAATCGGTTTGTAAAAAGTATATAAAGAAAGAGTGT

ATGATATTATATAATAAACCTACTTAAGTGTCGTAAATTATAAAGATTAG TACCGCGGGGAAGCAGTGGTAAACAAACGCAGAGTAA

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Rυn01_Cp312_MDl/Q2_Kubota_Hp35

_01_O18_287.F.esd 1490 MegaBACE

TGGAGTCAAAGCAGTGGTACATCGGCAGTAGTTAACGCCCANAGTNGTGA

GTCGNCGGCCGGAGGTAATCTTAANATTAATGNTTAATATNAATTAAATT

AACCTTATTTTTGTATATTGGTGGTAAGGGTTAAATACTAGACATTGTTG

GATTGAAAAAATTCTTTATTATTGGCAATTAATACCTTTTGACTTTGGAA

ACTTTGTNGAGNACNAGGTANATATCTAATTNTGNANTCCTATTAGATAA

TAANTCCGTTTTAACAATTATTCGGTCTTACACATTCCATTAACATGAGA

ACATTNGTAAGAAATACCATGGGAATCCTTAGGATCTGTGGGTTAAANAA

TTTGGGTAAATTAAATTCAGTAATATTGCCCTCTTAAATTTGGGAAAGAG

GAGACCTTTGAGCCCAGTAAATTATGNTGTAAGATGACAAACNTTGTGGA

TTAAAGAAAGTTCCCGCAATGTTATAAAGATTTTTCACACCCGATTTTAT

TTACTCTTCGGGTTTAAGGCACAAATGAAAAGAAACNTNGAANTAAAGAG

TTGTGGTTTACACCTCGTCTTTAAAANAGAATAAAGGTGGTGGACATAAC

GTGGAGAAGAGAAGGAGAATTTTTCTCCNAGAGTGAGCAAACAACGAACA

GAACACNNTTTGTTGTGGGTTTTCCCGCTTTTACAGAATTTTAAANATNG

CCGCNTTTTAATGTATTTTCTGAAGAAAGAAAAAGACCGANGTGGGAAAN

ACNCCCAAAAGGGTTATCACACGTAAAGTGTGAGGGCACTTATAAAGGNC

GAAGATAGCGCAANANAAATNTGGGGTAACACTTANTGGGTTGTATNTTA

AACCGCCTTTTTTGTAATAACNCACAGTTTTNTTCTGTTGGTAGNTCAAC

GTTTATTTAATTGGGGGTATGGGGAAAATTTACCATGTTTACNCACAAAA

AGNAAAAGTTGTCGGGNGTTNAAATGTCACAACANACGGTGAAAACGGGA

CAACAAACACAGTTGGNGGGAGAACGGTGGTCGGTGNAAAAACACATTTT

GGTGCGGTGGGATGTTTTATGAGGCGAGNTAGGTGTGTAGAGAACAAAAG

GGTGGTGGGAAGCAAAAAGAAGAGACTACANGTGTGGGGAAAACAAGCAA

AGAGTATATGTGGGCCTTAACAGGCAGATAATGTGTNNTTGAAACTATTT

TTGGGGAGCAAACAAGAAAAGTACTGTTTNTNGACAGCAANAAGGTTGTT

NGTGGGAAGACACANTTNAAAAAAAGGGGGCAGCACCNCCAAAAAATATG

GATGGGGGGCNACAGATAATTTATTATGGGAGGGGAGGGAGCCCAACAAC

ATTTGTTTAGGGGGTGTNATCACACCNCGAAGAGAATTTGTGATCGGGGA

NGGGGGNGGCCCCTCTTTATTANGGGTGTATTGAGCGGTGAGGGCACAAA

AGTGGGTTGGTAGGGAGGTGTAAACAATATNGANTGGGGG

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q2_Kubota_Hp35

_01_O24_383.F.esd 1381 MegaBACE

GGAATNGNAGCTCAAAGCAGTGGTACACGCANAGTACGCCCTCGAGCGGG

CCGCAATGGNCAGGTTACTTAATATTAATTTATTATTTGGNCNAATTAAC

TTCTTITATTTTACCATTTAATTTACACAAACCATTTTTCACTTGTTGAA

AAAGGNTATTATTAAGAATTAACAGGTGAAACTTTCAGTTCCGCCAAGGT

TACAACACAATATTTCATTGCTAATAGGCCACTTTATTCAGAAGGGTTTA

CAGTGACCAGGAAAGAAAAAATTTAAGTATAACAAATTATTTAACAGGTT

AATTCACTTGAAACATTCAAACACGCCATTTAAACCTTTTNCAGGGTTAT

AACGGATTCCCCCCTAAAATCCCTTAATTTCAAGTTTCCCAACAAAATCT

ACCAACAAGTTAGGGCACACAACAAATTATTCCAACCCAATTCCCACAAA

CATGGTTGGAACCCAACATTCATACTTCATGCCATGGCCCACTTTTGTAA

TTCACACAAACGTTATANAAAAAGGCCCCCCCTTTTAAAAAGACAAACCG

TTCCTGTTCGCAAACAGGAAACCTTTCCCTTNCCACAAAATTTCCCCAAG

CAACATTTTTCCCACAAACCAACAACCTTTGGGGNGGGTTTCTNCNCACA

AAACGCCCACAAAATTTNTTATTGTAGCCACAAAGTTTACCCCCCAAAAA TNNTATGTGAAAAATTCCCGTTTGGGCTTCACAACACGGTTATTGAGGGA

CACGGGCCTTATAAAAATTGNGTTTTAGGGGNGGCACCAAAGTGTGTTTA

AACCTNTTATGACTGCCCGACAAAGAGCAACGAAGAACACAATGTGTTTT

TGGGTTNTGAGGCAGCTTAAAAAAAAGGGGGANCACAAAGACATAACAGA

AAAAAACGACTTTTTACTTGCATGGTTAGGNGGGGGTGCTGGGGACACAA

CANTTTGTAAAAAACAGCCCGCCTTTTTAATATGGTNCGGTANGGGCAAG

TTTGTTTACAAACGTGTTTTGTCAGGGAAATTTACAACTTTTGTAAAAAA

TTTTGCCCGCCGACAAGTTTTGGGAAATTTGTCGGTTTTGGCGACCCACA

TATACACATTGCGGTTTGGGAGTTTAAAAAACGGGTTTTTNGNCAACGGN

GGGCAAACATTTTACACAGGAAACAGGGNCCCAAAGGGATAGGGGAGGGG

TATTATAAACCCCACTCTTACCCGCGAGAATGGGAACCCCGAGGCNTATG

AAAAAAAACCCAAATATGAGGTGTGAATCTTTAGNGAGGAGGAGGAACAC

GGTATTTTAACAAGTTGTTGTTGAGCGATATGTGGAGGTCAACGGAGGGG

ACGACTATNTGGTGATGGAGAACTAAGTGGA

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cρ312_MDl/Q3_Kubota_Hp35

_01_D17_260.F.esd 1213 MegaBACE

GTGAGCCCAAAGCAGTGGTACAACGCAGAGTAACGCCCTANGCGTGGTGC

CGCGGCGACGGAGGTGATNACCTTGTCTGCCTGGAGTGTTCAGACTGACG

TGGGGCCTAATGGTGCANAAAGNTTGGTAGTCGAAATTCANCTCTACAAC

CAGATCAATATACTNTCGTTGCACACTANNGCATACATACAGAGGCATGT

CTGGTCCATACCTCTCGCACATTGC AACCTAGAATTGTAATTCGCTCGCT

ATCCCAGCGCAAGTAAGAGTATGGAACACTATCCGTATGAGACCTCCTGC

GAATGTTGGTTTCGGACTGATATCTTGAGCGATTGCATAGTGTACAAATA

CGCCGCATTCTATACTAGGTTAGATATTGTGTTTTGGTCTTNTGGGTANC

NACCAAAGANAGAAGTTAAGTGAAAATTGTNGAACAAAAGCGCGCTTCTT

ATGCGAGGANAATAAATTGAGGGCGCAAGAATAGNTTGGGTTTGAAAAAA

TCCCTCCGGTGAAAGTTGAAATTGTNGGATAAAATCTCCAATNACCATTG

GGGAANCCAGATCACTGTGACTCGGGCCAANGCAGACCCGGCTCAANCTT

TATCCCTTCGATTACGGGCATACCATATGGAAGGACCGATTNACCATTTG

GGAANTAGAGATAAACACCACCAAGGTCACTCCTGTTGGTGGGCTCCCTC

TGCGCGGCTGTTGGTCACCCGGTTCGGCAANCCGGGGAAGGGTGTGTGGA

NCCCCGGTTTTTATATGAGGCGCGCTTGTTCCCCGGNTCCGGTTTTTCCC

GGGGGGTCCCCGTTTTTGGCGAAAGTTTCCCCCGGTTTGAGCGCTCCGGC

AACTTTAGNCGGGCCTATTATCGCTTCTAATGCCCCCTTTGGTGTTGGCT

CGNCTNNTGCTACCCACCCACTTTCTTGTGGTCTTCTNAGCATTTTGCGC

ACCGGGGGCGTGTTTTCTACAAAAANCAAATTAATATTGGGTCCCGCNCG

CCAGAGCGCCCAATTCTGNAGGAAGGGGCGCAATGTTTTTAACCAAGATT

AACACGCAACTTGCNGGGGGGGTTCGCGCCCAAAAAGTTCCCAAANGTTT

CCCCANTAAGGAATATGGTTTTGGCCTTCCCATTNTAGGAATTGGGCCTT

NCCGGTATGAACGAAACGAANTGCGTTANGGGTNTCCACCCTATNTTTTA

CCCCCTTGTGGGG

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q3_Kubota_Hp35

_01_D19_292.F.esd 553 MegaBACE

GATGAGCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGCC

GAGGTACCAAGAGGTGGTATTTGCACAGATGAAGGCTCTCCTCCCCAACA

TTTCTGACTGGTCACGTGTTGTCTTGGCTTATGAACCAGTCTGGGCCATT

GGAACTGGCAAGACAGCCAGCCCAGAACAGGCACAAGAAGTCCACGCAGA

CCTTAGGCAGTGGCTGCGGGACAATGTGAATGCTGAAGTTGCAGAGTCCA

CTCGCATCATCTATGGTGGCTCTGTCAGTGCTGGCAACTGCCAGGAGTTG GCCAAGAAGGGTGACATTGATGGATTCCTTGTGGGTGGAGCAGCTCTCAA

GCCAGACTTTGTTCAGATCATTAATGCTCGGGGTTAAGGTTTTTTTTTTA

GCATGGGGTTCAAAAGGCCAGCCACTTTCTTGGCTGCTTACTGCATTGCT

TTTGTAGAAGCAAGTAAAGTCTCTCTCTCTCTGGGCAAATGCTTAAACTT

CTTGGGTAATTGTATAAAAGGATAAAATGTGTCCAGAATTAGATAATTTC

AGG

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q3_Kubota_Hp35

_01_Jl l_170.F.esd 544 MegaBACE

TGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCGCCAGGCA

GGTACAAAACATCTCGTATGTCTATGGTGCTAACGAAGGATATCCAGACT

GATCCAACTATAGTGTTCTTTTTGGGAGAAAATTAAACACCTGGGGCCTC

CGAGAAAAAAGAAACTCATTTGGCAAAGCTAATGTTTAAGTCAATGCCTG

GACATCTTTCGTGCGGTATACTGTATCAAAGGTCAATCTCGAATATGTTA

TGGTTATTTTGGATGGGAGTGTCCTATGTGCAAGTGAAAACAATGTTGAC

CAGAAGAACTTGAAACCAGAATTATAATTTTAATAAATCTTTTTTTCTTA

CCATATCTTGAAAAAGGCCACAGAATGAGGGAGAGGCTTTATAGACGAGT

GGGGTTGAGAAATTAATGGAATTTAAAAAAGCATTTGGAGGAATAACATA

TAACGAAATAATGAAAGAGAAACTTGGAAAGAATATTGATACATATATTT

CTTATTTTATTCATATTATATCCCGAAATTAATTCTGAATCTTA

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q3_Kubota_Hp35

_01_J21_330.F.esd 699 MegaBACE

TGAGCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGCCGA

GGTACGACATGTCCAATATTGCAGATGCTCATGCTGAAGTTGACTATTCA

AGAACGACAACAGATGGTGATAAGAAGGAATTTAGGCTCAACTGGACTAG

GAAATCCACTGATGACCATCTTGAAAATGAAATGATCTTTGACTCCAACT

TTGAGACCCTGTCACATGCCCGTGCATATGCCAATGCTGAGTATGGTAAC

AATTTCAAATTGTTATCTGGGTTGGATTGGAATGACAAGAAAATTAGCCT

TACCCTTGAAGTTCGCAAAACCAAGATTTCAGGAATGCTCACCACACCAT

TTGAAGGATTTGAAACACTAGAGATTGACCTGCAGTATAAGCTTACTGGT

AAGGACAAGTCAGTTCAAGCAACATATCAACGGGGAGATAGGAAAGTCAG

TCTCAATATGGAAATGAGCATGAAGGGTAAAAAAGGAGGCTCCTTTAAAG

TTGATCTCACTACACCTTTTGAAGTGGTGAAAAACCTTCATATTGACGGT

CAGTATGAAAATAAAGTGGCTCAAATCAATTACCAGAGAAACGATATTCA

AATGAACTTCAATGGAAAGGCCAACATCAAGACAAGTAAAGCTTCCTTTG

ACATTTCATTCACACCACCTAGTGGTCAAAATATCAGGATAGCTGCCTC

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q3_Kubota_Hp35

_01_N03_046.F.esd 454 MegaBACE

GATGAGCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGCC

GAGGTACAATGCCATTTATTGAAGGCAAGTGCTTCATCACTTACATAATC

TTATCTTCCTTTCCACATTCTTTAAAGTGAGGTAGGTTGCTTAAAAAAAA

TATTGTATGAAAGAATCATAGTTGCAGCCAGAACATTTGGAGGGTTACTG

TATTTCATTCATAAACATTGATTCTTTGATACTTATCCTTCATATTTCTT

TTCACTTGTCTGTGTTGAATACATTACTCGTGGAATCCCCATTTTCTGTG

GTTTTATTCCAATTGCTGCTCATCCTGTGTTAGTCACATTCAACCATTAT

AACCTGTCGTGAGAGACACTTCTCTCAGTATCATATTGAGCAAGTGAATG

TAACTTGTATAAACAGCATGGTTAAATGTGAACAGTTGTAACAAAATACT

TTGT

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q3_Kubota_Hp35

_01_P05_080.F.esd 1480 MegaBACE TTGGAGCTCAAAGCAGTGGTACCAACGCANTAGTAACGCCCGCNGTGGTC

GGCGGGCCGGAGGGTACTTTTTTATTATTTATTTNATATTTTTTCAAATC

AANTAAGCACCTTAACAGGTTAACAAAGAACCTTCAAGAATTAAATTACA

GGACTTGGCCAATTCCAATTGGGGAAATTGCCCAATTCCTNCATTAAGGC

ATTTAACCAAAACCAGTGTGATGGCAATTATCCGGGGAAACCAAAAAAAT

TAAGGCATTATTATTGNGGCACAAAATTTTAGACAAAAAANGGAANCACA

AAAGGCAGAAGNATTACTTAAGTTGTGGTNGTTAACCAAATTCCGGAAAG

GGAANAAAGGNGGNGGAAATCTTTAACGGGTTCNAACGTTTGNGGTACGA

AAAAAACACAACGTGGNTTGGACATTTTTTCACATTTTAGANACATTTTA

AAACGAACACAAAAGATGTCCCTTTTTTACCCTTTGGTGTTTATCTGNGG

CAGGACAACATTTGGGTACNAAGGNGGAAAAGTTTCCTGTAGGTAAATTA

CCCCTGTTNNTCACTTCACGTGGGTANCCATGGCCCAAGTGTATGAGAAA

CCAATTTTAAACCGNGGCCCCCCCGGGATGNGTTAAACCCCCTTTTGGGC

CCCCTTTGNGGGACTGGNGGGCCCCGNGTTTCCGCGTAATAGTGTGCGTG

TTACCTTTTATACCGTNNGGTCCCGGTTCCTTTCTGTGGAAAGGGCCCGG

TTTACACCTTGGGACNTTTGNGCGTTAACCCCCCAAAGGACTTATTTTGT

GGGGTATACACCCCCCTTTTTTNCAAGGGTTGCGACACGGGGGTTATAAN

AACTTTTTTAGGGACATNGACCCTGCACACTTTATNAGGGGGCCGGTTNA

AAACATTCCACGAAGTTGAGGGGGTTCCAGAATTTAAAGGGCTTTATGGG

GTTATNTACACCCTGTTAGGNTTAGGGTTTCATGGAAACAAATTTGGGGT

GNCAAATTTANCCCAGCTGGGAGATTACAAAGCACAATAAATTTTCCGGC

AAAGCAAAAATCGAGAGAGTAAATTAAAACCAGAGAAAAGAGCCCCNAGG

GTGGAAAAGGCCAATTTAAACAAAATGGTNTTGGGTTATAAAACAAATNG

AGGCCTATTATGNAGGAGGGGGGNATAGGGGCCCNTTAAAAATTTGGGAA

GTTTTGGAAAAGGNTTATAAAAAGAGTGTTTCACAAGCCGAATTTNTTAC

AAGATTTNTGGGGGGTGTATATAGGCGGGCGCTTTAACAAGTTTNNGGAG

CCCAAGGAGTTATTTATACCACAAAGGTTTACCTGGGTGGTGGAAAAAAA

CCGTTTTGGNTATAGAGTATATAGACGCACAAAAAGGTTTGGGGGCTCAT

TTACAATTGTTNGGAAACTATTCCNGGGGGGCACAAAATATAGTGAGCGC

AGCTGAGCGTGTGTGGGAAANACAACGAGG

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q3_Kubota_Hp35

_01_P07_112.F.esd 1451 MegaBACE

GGGAATTGAGGNNCAAGAGCNGNTGATTAACAGGCGGTATTAAGCCTTNA

AGGGGGCANAGGACAGGTAGCGGGGCAGTTTAAGATATNTTAANGTTACA

GGTTANTGTATCTAAACTTTGNGANTAAGANTAATTTTTTATAGTGATGG

TGGAATNATATATAANATATAATNCNTTACCTTACATGAGGATAGGGGTA

CATATAAATATTACCGACACTGTGANGAAAAGGTNTTATAAGAAAAGGAA

AGGTANTGTTGCACATAATAAGGTTACCCAGGGAAAGTTAATTAATACAC

AGGTCTANNTAAGNGTTAACTATACGGGGCGCGGGGAACANAAAGGACTN

TTGNGGGGGCNGNTTTAAAACTTACNTGACGGAGGACAGCGGANGACTTA

TGGACAAAGGNGGTTAAGCTTGNATATTGAGGTTAAACACCAAGGCTAAA

ATATGAGGATAATCCCCTTATTAAAGTTTAGAANAGGTGGAGGTTAAAAA

ATTAGAGGGGGCTCAGANCTATTNTGAGGGGTTAAATTTCGANATGAGAG

GTCCCGATTAAAGGAGCTTGAGTTTATATACCACCTTNNAGGTNGGGTTT

ACGAGAAAAATTNTGTGGGATTACAAATNCCCTCNGGGTTTCTACCNCAC

ACAGATAATGTATNCAGCTACCGACTGAAAGAACAAAACGAATATTTAAA

ACGGAAAGGGACCCGTGGGGGGGAANAGCCAATTNAAAACAAGGGTGTGA

TAAAAAAGAGGGCCNTCTGTGGAGAGAGGTTAAGCCCNTTTAANGATATT

GGGGAAAAGTTTTAGGGAAAAGGTTTCAAAAAANATGTTTTCANGAAAAT CACTTANTTTATAAAGATAAGTTTTATTGAGGGACGATGTTTTAAAAGGA

GAGGNGGAGCNTGATAAGACAAAAAAATTTANGAGGGGAACACAGTAGGG

TTGTTATATTATAGAACNGACGAAAGTGTTAACCTGAGGGNGGGAAAAAA

AAAACGCTTTTAGAGNTGTCAACCCGGGTGGAGAGGACCACAAAAATGGT

TTAAGAGAGGAGNTNTTAATAAAAAAATTAGNGGAAAAAATNTATAATCA

GGTGGGGGGCCNAAAAATATAAANGATCTAGTACCTAAGTGTAGTGAGAG

AAAAAAACGGAAAATNGNGGGCNAGGGNGGATTATTATAANGGGGGAACG

GNTGTCAAATAATTGATNAGGANGGGGNAGGCAAGGGACNCTAAACATGT

TTAGNAAGCACGAGGGAGGTGTTTAAAACACATCGTTATAAAGGGGTGTT

ACANNAAGAAGTTAGTTGAGANGAAAATTTGNTAAGTACGTTTTTGAGAN

AGAGGAGGANCTTAANNAGGGAGAGTAACAAGAGGATATCACACAGGGGG

NAGTTTAATGAGCTAGATGGAGGTAAGAGGAAAAGGGCCNAGAGGNGTAA

A

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_C_P312_MDl/Q3_Kubota_Hp35

_01_P09_144.F.esd 723 MegaBACE

GTGAGNCAAAGCAGTGGTACACGCAGAGTAGCCAGCGNGGTCGCGGCGAG

GTACAGGANGCGCAGATTAATTAAAGAAGTCTGAAGAAAAAAAAATCGGT

GTATTGCTGAAATAAAAGATNATCTGAGAAAAAATCCAGTGTAATGCAGG

AAATAAGTGCATCAAGTTAATTAGTAAATTAAAAATATGTCAGGTTTCTT

TAGAAATTAGGTCTTTAAAGTTTTGTAAATCATCTAGGGTGATGACCAGG

TAATATGAATGTTNGACTGATACACTTTTATCTTAATTCTTTAAGACCAT

AAATTTTTATTAATNCAAGGAAANGGAATTGCCTATAAGGCAGGTTGGAA

GACAGGGCTGATAATTTGTATTATATTTAAAGGAAACTAATACAAAGGGT

ATTTCCTAAGAAAAATCAGCACTTATCCTTTAGTTATAAGAAGCCATCTT.

AAAGTTANATTAACAAAACCATTGTTTGGGAAGANTCACTTGTTAGTTTT

TGGCAATATCCCTACAATCCTGTTTTACTNTTCTTGTGGTGGGAAAGGAT

GGGGAAGAATAGGTAACCCTTGCCCCCTTGGCGGGGCCGCTTCGGAGAGG

GGCGGTTACTNTACTTGTTCGGTCATAGGANGCCGTACTTGGAATGNGTT

ACCCCCAAGCTTTTNTGGACCCCCTATTTAAGTTGGAANGGGGGTTTAAA

ATTTAGGCGCACGACCTTTTGTG

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q3_Kubota_Hp35

_01_P13_208.F.esd 730 MegaBACE

GATGAGNCAAGCAGTGTACACGCAGAGTAGCCTCGAGCGGCGCAGGCAGG

TTGTACTTCAGGGACCACAGAGTTGGNAAATAAAACCCGACCNAAATTCC

CACTGCCAACCTCCCANAGTCGGACCCCAGATGCCATGCAANTGGTTGCC

ATATCCNTCACCCGACTTGATTGACATCTTAATTAACTTAATTGGTGATG

GTGAACCAAGAAACAATTTAACCTACTTNAGTGCTCGTCTGGGGCTAGTA

TTCCACCCGGCGAAACCAGGGAATTACCGTTGGTGGTGGCCCCGGTTCAA

CCCAAGTTAACCTTCCGGGACCGGCAGAGAAACCCAACATCGCCTTGGTG

GCCGGTTAACCTTTCCTTNGTCCGGTCCTTTTGGAAGCCGGTTAACCTTG

GAATTGGGGTTAACCCCAAGGCTTTTΓΓTGGTTTCCCCCTTTATTAAGGT

TGGGAGGGGCGGGTTTAACATTTTTNGGCTCGGGCCTGGCACTTTTTGGG

TGGCGGGTTTAAAAATTTCCAAGTTTTGGGGGTTCCAAATTAAAAGGCTT

TGGGTTATGTTACGCACTTAAGGGTTGGTTTGGAAAAAAAGTTGTTGGTT

GAATTNCACAGGGGATNCCAAAACGAAATATTTTTCGTCACAAGANAAAC

NAATNAAAAATTTAAACCGGAAAAAGAGCCCCAGGGTGAGGAAAANGCCA

TTTAAAAAAAGGTGTTGGGTTTACAACAGG

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q3_Kubota_Hp35 _01_P15_240.F.esd 344 MegaBACE AAGCATATGAGCCAAAGCATTGGTACCAGGCAGNATNATACGCCCAGNGT

GTGGTCGTGGCCAGGAGGATCANGGCGGGGNCCTCTAAATTGGGAGGGTT

TTGATNTTNAGATTTTGGNTTATNGGGNACCACAAAAA AAACATACAATA

GAGACAAAAAAAAAANNCTAGTCCTTACAAAGTATANTTAAATTTTTAAG

TATGAAATTTTGGAAGCATTTTATAAAGAGTATTANTTAAAAAAGGGTTT

TGAAAAGAAAGAGAATAGAGAATTATTGNTNGGANGGGAGAAATTANNAA

ATAGNGGAGTTTTTAAGAGTTTATACAAAGATGTGGGTGGTGAC

>E:\AnalyzedData/50425_4_2_Q l_Kubota_Hp35_01 RunO l_Cp312_MD l/Q3_Kubota_Hp35

_01_P17_272.F.esd 717 MegaBACE

TGAGCCAAGCAGTGGTACACGCAGAGTACGCCTCGAGCGGCGCATGCAGT

ACGCGGAAGCAGTGTAACACGCAGAGTACGCGGGGCTTCNTGANGGTCGA

AGGAGNTGTAGGACGCCNGGAATTCTTCANGCACAAATATGTTGAAGGTG

GTTGTATTGGAGAANGCTGGGAGGTTGTTTCCAAGGAAAGTTTGAAAGCG

GCCACCGNTTTGGCAGTTCTTCCTCNTTTAAGGAANTTATTCTTTACTTA

AAGGGAAAGTTCTTTCCGGACCAAGGTTCCGAAGGACCAGAAAGACCTTC

CCTTGGGGAAGCCACCCTTCCTTCGGAACGTTCTATTCCCCAGTTCCCGG

GTTGTTGGGGAAGAAAAACCAAGGGAATTCCTTNGAGTGGGTTAGGGGTA

ATTCTTATTGNCTTCCCTGGACTGGCCCGGAAGGGCCTTAACAACCCTTA

CTTNTCTTCTTCCCCTTCCTTAGTCCGGAACCCCCCATTCCAATTCCGGA

AGGGAAACTTAAACCCANATTGGTTTTAGGGCCTTTATTCCAAGGGCCAG

GAATCCCGGNAANCTTAAAGGGCAGACCCGCGCCGNAAAACGAAAGNGGG

GAAACTATNTACCGCGGGGTTGGGAAATTTGTTTGGAAAAACTTCCCCAT

TTATCNGGTTGGGANAACCCGGTTTNGGAACCCCCCGGAAAGGGGGCCCA

AAAAGGGTTTATCCGGG

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q3_Kubota_Hp35

_01_P23_368.F.esd 581 MegaBACE

GATGAGTCAAGCAGTGTACACGCAGAGTACGCCTCGAGCGGCGCCAGGCA

GGTACCTCATGGGACACCAGAGTTGGAAATAACCNGACCAATTTCCCATT

GGCCCACCTTCCCAACAGTTCGAACCCCAAGAATGGCCCATTGCGAAATT

NGTTGGCCATTATTCCCTCAACCGGAATTNGATTGGCCCTTCCNTANTAC

TTAATTGGTGATGTGACCAGGAAAACTTGTAACCTTAACTTTCCAACGGT

TGCCTCCGTTACTTAGGGGCCTTAAGTTAATTTTCCCAACCCGGAAAACC

AAGCGCATACCGTTGTTAGTTAGGCCCGGGTTCAACCCAGGTAAACCTTC

GGGCCGGTGAACCAACGGCTTTGGGGCGGTAACTTTCCTTGTCCGTCCGT

TTGGAAGCGGTACTTGGATTAGCGTTAACCCCAAGCTTTATTTTNAGGTT

ACCCCTTTTTTTAAGTTCGGGAANGGGGTTTTCACATTTTGNGGCGGCGC

CTTTNGGGGGTGTAAAATTCCATNGGTTCCATACAGCCTTGTTTATCCCT

GTGTTGTTGAACNTTTGGGTTCATCCCGTGG

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q4_Kubota_Hp35

_01_B04_050.F.esd 625 MegaBACE

GTGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAAGCGGCCGCCAG

GCAGGTACAGCCTCCTACTCCTCGGAGACGTGTTGCTATGCCGATCAAAG

CCAGCACGGGGAGTGGTATGACTTCAACGAGTACAAGCACCCCGACTTTG

CTGACCAGTTGAGTCGGCTGTAAAAGCATGGTCGAGCTATGGCACCTTCC

ACCCCCTCTCTCATATGTATGTTTTATTTTTTACTTTCCGTTCCCCCTAT

TTTTGTCGCCAGAAATGTTTTTTATGCAAATTATTTTAATAACAAGTTTT

TTGGAAAATAAAGTCATATATCCGTCCCTGTGAAAATTCTACTAAAGTAA

ATATGTTTAATAAAGAGAAAGGCAGAGAGGGGGGGAGGAAGTAAGGAGGA

TATATAAACAAGCAGAGTGAGGAATGGAGAAAGAGATAGATAGGCGTGTT TGTTTACACAAATGGCTCTGAATAACTGATATATCTACCCTCAGTCCATG

ACGCCAGCGCCAGGCCCGCAACACCGCAGCCAGGAACCTGCCAGTTCCCA

TTTTCCTCAAGACCAGTGGGAGCGACTACGAGGAGAACAGTCGTCGTGCT

TTTCAGTAGGCCACGTGAGTGCCTG

>E :\AnalyzedData/50425_4_2_Q l_Kubota_Hp35J) 1 RunO l_Cp312_MD l/Q4_Kubota_Hp35

_01_B12_178.F.esd 391 MegaBACE

GGAGAGAAAAAAACATAGTTATNGGGGNATTNTATGNGANTGNTGTTGGT

NATTCAGTAATGNAAAGTAGTAAGTAGGGTGCCCGAGAGGGGGTGGGTTT

TTCAAGGGGGGGNNAGAGANGTGGANTGCAAGGGANTAGATGCGNTCNNG

TAGNGTGGATAAGCTGTTTTTGAGNAAGGAGGTGGACGGATGGAGNNAGG

TAAGACGAAGAGGGAAGATTAAACATGAGAGAAGGTNCGTGTGAAAGGGT

GAGATGNTAGANAGTGNGAAAGAGTAAAGNNGAGTAGANTAGACGTGGAG

ATGATGAGGAGATTGAATGAGTGATTGTGNAAGATGAANAANAAGAGTGC

AGTTAAAANGNAAGGCGTGTAAGAGANGGCGATTANGTGGG

>E:\AnalyzedData/50425_4_2_Q l_Kubota_Hp35_01 Run01_Cp312_MD 1 /Q4_Kubota__Hp35

_01_B24_370.F.esd 1720 MegaBACE

AAAATGGACACTANGAAGCTCAGTNGNTAACAGCCGGCAGTAGGCTNATC

AGACCCTTGTCGTTCGGATGCTGCTGAGAACGGTACTGATATCGGCGCGC

AGACGTGCCGGGAACGTGTCTGACCTTCTTCGAGTGACAAATATGTGCNT

GGCGNATTTCTCCACTTTCCCCTGTTATTGCTCACTCGCGAACTGCGTGA

CTTGTGGCTAACAATAGCAGTGCCGGNTCATCATGGAACTTNGTGACTAA

CGCTGTGCAAACACATAATGTAGGAGGTAAACTTGCACTTGAAGNTTTGG

TTCATCACAAATTTATTAAATGAACACCAATAAAAATGCAAAATTTGNCA

NGCAAGTTGGTGGTTTCCCTCGGGATTNCGGAAATTCCGTGGTAAATTCC

GGGCATTTTGGTAACTGGNCCAACTATTNGNTTGGAATCTTTGCTTTTGG

TCGCCAACTGGTGGGGTTAAACCCAGCGATTTTGTGGGGGACANGAGACC

CTTTTTGGGAGGGAACATGGTGTCGGGGTAACCTATTGGGAATGAATACA

CCCATAGGGAAAAATGTAGTTGGGGGCTGTGGACACCTTGCNGGATNTTA

CACAGCGAAATTTATTGAACCACAGTTTAGGGNNAGTTAACACCCTGTGT

GACAGTTAACATCCAATTTTATCNTGTAGAGGGAAAAAAAGAGTGGACCT

ATGGGGGTTTTAACAAAAGCCAATTTATATGGGGGNNAAAATTTTATCGG

GATGGGGCATTTATAAAAAAGCGATGCCANCAGNCACNAAAAAGAGAGGG

ATCACANTTAAAAATAGANAAAGACACTTTNGTGGGATTATCCATTAACC

AGAACATACCAGATTATAACTTATATATCACAAGGAGGTTTTTTAGGGCG

AAAATTGAGCCGGGGTGGGANATTACTTCGAAAAGAGTTGTCTTATCAAG

GGGCCTAATAATAAGACCTTGGTGGCNCCATANTATTTNTACGGTTTTAG

AGTTANTTGGCGTTCAAACGAAGATTTCCCAAGAAACTTTTGGTGNGGNT

ATACCACTGAGAATCTACAANATGAGGAGCCGTTAANGGTCGAGATTTTC

CGCCANCACACTGTTATTNTGGGTTATCGTGGTTTTTCAGGGAACACATC

AGAGTGTAATCATATNGGTTGCTACAAAATTTCCAGCCTCTAGGACATTT

ACCAAAAANGNAACAAGTTTGTACCNTACGAATAGACGAAAGCNCATATN

AAAATGCAATAAATTAAGAACACCTAGTGACTATTGAACGGCAGTNTGTT

CTGGNGACATTGCGGAATTTNCAACANGGCAAGAGGTGTTGGGTTTTAAA

ACACATATCGANAGATGAGAGAACACCCCATTATTANGAGAGGNGAGAGT

GCGGAGAGGAAGTTTTATNGGGCCAGCACCNGTATNAACAAAGAATTTTC

TGCAAAACGTGGGNTTCTATGGAAAAAACGANCACTACTGCTAATATGAG

ACNANTTTTACCCACAAGAAGCAAGAAAAGTTTACTAANCAAGAAAAAAG

ATGTTAATGATTGGACAAGCAACAGCGATATAATACTAGGGGCTGATANN

TGGACCTAGATAGTACCACCAAAAAAAAGACGTATNTTTGGAAGCTTATG CGATTAGANGAATGGACGACAATTGTAATATATACCAACGCNCANAAAAA

GGGAAATTCCCAACGCAGGT

>E:^'\AnalyzedData750425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q4_Kubota_Hp35

_01_D08_116.F.esd 1515 MegaBACE

GGGCTCAAAGCAGTGGTANCAACGCAGAAGTACGCCCAGGCGTGTGGTCT

CGCGGGCCGAGAGGTTACCAAATAATCTCATATGTATATACGATGGATAG

CTATTTGTTCAAATCANGGGTACTAGAGACATCATAAAATTCGATGTCTG

AGCATATCACTACATGCGGGTNTCCAAGCTCGTTTCTATCAGNTGATAGC

ACCATTGGACATACAGGAGCTCGATCGCACAAGAAATCTTNAACAGTTCA

TCCTATACACGCAACTGTACACCAAGGCCATGTAAACAGCGATCCATAGG

TCAAGGATTCCATGATACTATCNCGTATCCGACAGAGTATCTCTNNTGTC

ACTTCCTCTTGAAAATTCGCNCATATAGTAGCTAACAGAGACGTGGGCGC

AATATNAAGAGATTGCGTNTCTTTCTCGCGCAATGCGAGTCTATAGAGAA

GTTATGCATTAACTCGTGGGCCAACCANCCTTAATCAACATCCAAGCTGA

AGTTTGTGGCAGGGCCCGTAGCGCAGAAAGTCGCGGTAAGAAATTTTGTC

CCAAGGATCTTGGCCGACGACTAATNGTTCGNACGGCCGCCATAGGGCGC

CAATAATGCCACCTANGAAAAAAGTACCACTTTAAACGGCTGCCAAAGGG

TTGAAACTTCCATTGGGCTCCTCCCTAGAAGAACCCGGAGGGACAACTGG

CCCNTACCTCGCCATTGTGGCGACTTGTTTTAATCCCTTTTAACGGTAAG

TTCCCCCCGTTTTCTTTTTGGGACACTTGCCCCAGATGTGATTCCGGTCC

GCCTCATTTTGCCGATAAGGCCACTTTCCTTATTGAATTTTGGCCCCTGG

GGTTCCTTCCTCCTCCATTTTTGGTCCCCGTTTTAAAGGCAAAAGCNGTG

TAGGGCTTCCTTAAGGATTTAAATCCCGGCCCTTTGGGGCCCCGCCCCCT

CTTTTTTGGGTGGGGGGCCGCGGGGGGCNTCCCACCCNGGGTGCGCTTTA

ACCTNCGGGGAAAGGGGTGNCGGGAGACCTCTGTATGTGAACCCCTTTTT

GGTGCTCCTTTTAGGGCCGTTCTCCTGGGNGTTTTCCNGTTTTTTTTGGG

GAAAAACGGCGACCCTTGGGCTTTGGAAATCCCTTTTATGGTTACNACTT

GCCNGGGGGCGTTTTAACCCCACCCCCACAAAAAGGTGCCTCTCCCTCCG

AAATTCGGNATTCCTTATACCCACCCCCCCATTTTTTTTAATNCCTCCAG

GNGACTTGTTGGGCGCANCCCGGGCGCGGGTCGGCGTTTCACACTTCGTN

CCACCAACATTTTTTTGTAAGGGCCCGAGGGGGCCCTGGGCNCCCTTAAA

TATGAGGNGGGGCCGCGCGTTTGAAGAAAAGAGTTTCCCCGCATATTTTT

GGCGGCGCGCGCGNTTTCCCCCNAAAATTTTTTAAAGGGAACCCGGTTTT

TTGGGAANAAANGCTCCCNTCNCATATAAGGGCGTTTTCGGCGTTATAGG

GGCGAAANATATTCA

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q4_Kubota_Hp35

_01_F14_214.F.esd 525 MegaBACE

GATGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAG

GCAGGTACGCGGGAGACTAGTCGTTTCCAAGCCTCGCCATGACAGAATCT

TGAGTATCGTGCTTGCTGCTTTAAAGAAAACCCTTCGTCGAGGTCTTGGC

GGCAGAGTCGTTGTCTGAGGAGAGGGAGCATCAGCGAAGTTGTTCCAACA

TGTGGCTTCCCATGCTGGTTATCCTGGCTGGCGCGNGTGCCTCTGTGGTG

AACTGCCTGGAGGTGTGCGCCCCCGACTGCACCGGCAGAGCGCCCGGCGA

GAAGGTCGTTGACCCTAAAGACTGCACTCAGTTCTACTACTGCCTCGTCG

GTGAAATGCCGACCGACAGCCCTGTTCCGTGTGAGGACGGTCAAGTTTTC

GACACTGCAACCGGCTCCTGCGTTCCCGGGACAGACTGCACCAATCTCTG

CAGTTCCAAAGAGTGTAACTACGTCTGCAACGATATTTAGAGGTGGTCAG

CGACCCCCTGAATTGCGGTCAGTAC >E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q4_Kubota_Hp35

_01_J14_218.F.esd 586 MegaBACE

TGAGCCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAGGC

AGGTACTTCTTGACTCCCTGTGGGTTAGTAATACCTAGGATAAGCATGTG

TTCTGCTAACCAGCCCTCGCGACGGGCGATGGTCGACCCAATGCGCAGAG

CGAAGCACTTCTTGCCCAAGAGGGAGTTTCCGCCATACCCTGAACCGAAG

GAGATGATCTCGTTTGAGTCTGGCACGTGAGTGACGATCGTCCTCTCGGG

GTCGCAAGGCCAGTTGTTCACCAAGGTCCTTTTGAGGGGAAGTGGGTAGC

CCACAGAGTGGAGGCATTTGACGAAGTCTTCTTCAGCGAGCGTATCGAGC

ACGTGTTTCCCCATCCTCGTCATGGTGCGCATCGAGACCACAACGTACGC

CTTGTCGGCCGTGATCTGCTTGGAGGGCGGCCTGGACCTGCAGCAGGAGC

TTCACCCTCTCGATGGGCGCCACGGCCGTCTTGGAAATCGCGGCGGCGAT

GCCACCGGCGATAAAATCCTTGGCGAAGCTCATAGGATCGAAGCCCTTCG

ACATGATGGTGGAGTGTGTATAGTAGCGTCCTTGTC

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q4_Kubota_Hp35

_01_L08_124.F.esd 554 MegaBACE

ATGAGCCAAGCAGTGGTACACGCAGAGTACGCCCAGCGTGGTCGCGGCCG

AGGTACAGCGGGCGACAGGGCGTGGGCGGATGCAATGGTGACACAGGCAG

CCCCCTCAACTACGTGAAGGACGGCGTCACCTACACCCGCGGCATCGCTG

TCTTCTGGTCCTCCGCCGGCTGTGGCTCTGGGGACCCGACGGGCTTTACT

CGCGTCTCGAAGCACCTGGACTGGATCTCGGAGAAGACTGGCATCGCTAT

CGACCCCTGAGGCTGAAGGAGGGCGTCCCCGGGGTGGTGTCGGTCATCCC

TCCTTCCCACCTTCTCATTTCTCACCCTCCCTCCTTTCCCCTTCTCACTT

CCGCCCCTCTTCCCCTCCTTTCCCCCCTCCCTCTCTCACTTTCCACGCTG

TGGTTTGGTTATTCTGTATATATATTGTTGCTCTTGNTATATATATATTG

TATATAAGTTTATGTATGTGATGTAAGAGGGAGAGAGAGCAGAGAGTGAG

AGGGCAGAGCANAGAGAGAGAGTGAGAAAGAGCAANGGGNCNGATGGTAG

GATG

>E:\AnalyzedData/50425_4_2_Q l_Kubota_Hp35_01 RunO l_Cp312_MD l/Q4_Kubota_Hp35

_01_L18_284.F.esd 319 MegaBACE

GTGATAGAATAGAAGGGTTGTGATGACCCGATAGGGGGGTTTTCAAGGAG

GCTAGGAATGGTNGTGAAGTTAGAGGNAATGAACCNGAAGTGGATNAANG

TGGNATTTATAGGAATGAATTGGATAAAGNTAANTATNCGAGTAGAATGN

TTGGAGCNATTATGTTCTTGANGAAATAGAGCAGTGATNAAATCTAAATG

AGATATATGAGATTNGAAGAAGTAATNATCTNGTTAGGNAGAGTNGTGNG

TTGGTANAAGAGACANTGTGGGATAGNATAAGATAANTTATNAAGTAAGN

AGNAAATAAGAGTANGTAC

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q4_Kubota_Hp35

_01_L24_380.F.esd 733 MegaBACE

GATGAGCTCAAGCANTGGTACACGCAGAGTACGCCTTNTGGCGCTCTTAC

CGTTTCTCGCTCATGANTCGCTGCGCTNGTTCGNTCGGNCTGGGGGCGGA

AGCCGTTANATCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTCG

GAACCGGCTCCAAAGGTTCAGAGGTGGGCGAAACCCCGACCAGGGACTTA

TAAAGGAATACCCAGGGCCGGTTTCCCCCTGGGAAAGCCTTCCCCTNCGG

TGGCCGCTTCTTCCCTTGGTTTCCCGAACCCTGACCGGTTTAACCCGGGA

ATTACCCTTGTTCCCGGCCTTTTTCTTCCCCTTTCCGGGGGAAAAGCGGT GXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXCAAAGGCTGGAGGCTTGTGTTGGCCACCGAACC

CCCCCGGTTTACCAGGCCCCGGAACCGGTTGGCGCCCTTTAATTCCCGGG

TAAACTAATTCGGTCCTTNGAAGTTCCAAACCCCGGGTTAAAGGACCAAC

CGGACTTTTAATTCCGGGCCCAACTTTGGGGCA

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q4_Kubota_Hp35

_01_N06_094.F.esd 526 MegaBACE

GATGGAGCTCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCC

AGGCAGGTACCCCAGTTGATTCAAAGGCACAGGCGCGTGTTGATGAATAC

CTTGAGTGGCAGCATGTGAACACCCGACTGCAGTGTGCTATGTTCTTCCA

GTTTAAGTTCCTGCTACCAAAGATGCTTGGGATGCCAGTGAATGAAAAGA

AAGTTGCGGAGTTCAAGGGACGGATGGAAACTGTGCTGGATCAGCTGGAA

ACCATTTGGTTGAAAGATCGCCCATTTATTGCCGGAGATCACATAACCAT

TGCTGATTTACTTGCTGCTTGTGAACTGGAGCAACCAAGTATGGCTGGGT

ATGACGTGTGTGAGGGAAGGCCTAACCTGACTGCCTGGTTCAAACGCGTC

AAAGATGCATTCCAGCCACACTATGATGAAGCACACGTGATGGTATACCG

TGTCAAGGACAAATTTGGTGGCAGTCCCATGAAATCAAACCTTTAACATG

AACGATGGTGTATATAGAGTTATGGA

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cρ312_MDl/Q4_Kubota_Hp35

_01_N10_158.F.esd 500 MegaBACE

TGAGCTCAAGCAGTGGTACAACGCAGAGTACGCCCTCGAGCGGCCGCCAG

GCAGGTACGTGGGCATCAACAAGCATAACGCCATCAATTTGAACCGCGGC

AGAAGGATAAAGGTGTCCTTCAATGTTTATATTCTTGAGACTACGAGCCT

TGAAGTTTCCATTCATCTTGAGTCCAAGGGTAGCTGTTCCCTTTGCAGTG

AGGGTCACAGGTAAACCAGTCACAGTAGGAATGGTGTGGTGAGTGTCCAT

TAGCTGGTAAGATTTTGTGTAATCAACCTCTCCCTGACGAGCCAGATTCA

TCAAGAATTCCAGTGGGTTAGAAGCACCGGATGTTTGGATGAGGTTTTCA

AGTCCTAAGAAGTGGTGGTAATGAAGCTCATTGCCAAAAACGCGGAGGTA

GTAGGATCCCTTCGGTTCATCAGTTATGTGGTCAAGGCAAGCCTTCAAGC

GTGGTGGCATCAGTGTCACTCCCGCGTACCTCGGCCGCGACCACGCTGGG

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q4_Kubota_Hp35 _01_N24_382.F.esd 677 MegaBACE

ATGAGTCAAGCAGTGTACACGCAGTAGTAACGCCTCAGCGGACGCCAGGC

AGGTACACTGTGCCTTGTATGTGTGTTGGAGAAAGGTTGAGAAGAAAGGA

ATTAGAAAAGTCATATTAACCAATTCTGAAGGAGGCTGTTGGTGTAAATG

GAAGNCAAAGAACCCATGGCCATTGTTANTCCGGAATGACCTTCTTTAAG

GTGCCGGTCCACAATTAATTACCTCGGCCGGAACCACGCCTGGGCGGTAC

TCCTGTCGTCCTGGAAGCCGGTACTGNTGGTACCCAGCTTTTGTGCCCTA

TTAGTTGAGTGGTTAAATΓGCGCXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXGGAGGCATAAAAAGTGTAAAAGGCCCTTTGGGNGGGTTGGCCTTAAA

TGGAGGTTGAAGGCTTAAAGTTTCCACCATTTTAAATTTGGGTTTGAGGC

NTTCAAATTGGNCCCGGCTTTTATCCCAAGTACCGGGGGGAAAAAACCTT

GTTCNGGGTGGCCAANGCGTTGGCATTTTAATTTGGAAGATNCCGGGGGC

CAAAAAGGGCGAGCCNGGTNGTGGGACAGGAANGGGNGGGGTATATTNGC

GGGTAATTTCTGGGGGGCCGGCTTCTT

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Rm01 Cp3l2_MDl/Q4_Kubota_Hp35 J)l_P02_032.F.esd 835 MegaBACE CCCGAATGAGCCAAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCC

GCCAGGCAGGTACGCGGGGGTCCAATAGCGAGAGTAACTAGGACCTCTCC

CTTCCCCCCTCTAGTTCATGGATCCCTCCCAGGTGAGGGCTCAAATACTG

TGAGAAATCGGATTCTGCTACGTGTACGGCGGTCCTGTTCTCCTCGGGGC

AACGAAAACCACACGCTTTTCAGGCCCGCTATTGGCCGGAACGGGAAACC

CTTCTTGGTTTGGGGGAACCTTGGACCCTTCCAAGTTCCAAGGGCCGGTT

TCCTTTCCGGGGTTGGAATTATTGGTTTGGAAANTGGAAAACATTTCCCA

AACAATAAAGAAACCACAACATTCCCCCCCCCCAAATTGGAATTAGGAGG

GAACCTAAGAAGGTTGGCCCGGAACAGGCTTTTCCCTTGGAGTTCAACCA

AGTGGCCACGTGGTTGATGAAACCACGACCACCAATTGGAAAACCATTAA

TTAAAACCGAAGGTTTGGTATTTGGGAGACACAAGGAACCCAACTTTGTC

ACGCAATTAGGGTTTTAAAACAACTTAAAANAGGTTAGGGGGCCCCAAAA

AATTTTNGGGAAACACATTTAAAATTTGCCTGTTTTGGGGTGTGAAAACT

TTTTTGGGTTTTTTGGGCAAACCACAAACATGGGGGGGAACANACACGGG

GAAAAAGGGTTTTAAACTTTTATTTTGTANCCCTTTATCCCTTTTAGGGG

ACACACAAAAACAGGGTTGAAAATTTTTCCCAAAGTTTNTCACCCCAAAT

TATGGGGGGGGCGNGTTTTAACNGGGGGAACANAG

>E:\AnalyzedData/50425_4_2_Q l_Kubota_Hp35_0 lRunO l_Cp312_MD l/Q4_Kubota_Hp35

_01_P08_128.F.esd 479 MegaBACE

GTGAGCAAGCAGTGGTACACGCAGAGTACGCCCTCGAGCGGCCGCCAGGC

AGGTACATTATCCCCCAAAATGGGGCATTTAATATAGCATTGCTTACTTA

CAATTTCATTTTTTAATATTGTCAGATTTATCACAAAATATTCTCATCTT

TTAGTGCCATAATTATTCTATTTACAAACATTCCATAAGTGGATTGCTTT

TGGGCTTTCCTAAATTATTCAATTCGATGCCGAGCAAGTCGAATGAATCT

GACAATTAATAATATAAGAAAAAAAAACAAACAAAAAAACACAGTTTTAC

AAAAATCACAGTCAAAAGGCACCGGGACAATATGCAAAATTAATTATTAT

CAAGATAAAAAATCTACTTATTTGATTCTTTACCTTTTCCATTCACACTT

TGTCCTATTTGAACTATTAATTCATCTGTTTATTTCCTAATATTTTAGTA

ACTAAAGAAACATCAAGCGAAAAATGATA

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q4_Kμbota_Hp35

_01_P18_288.F.esd 754 MegaBACE

GATGAGCCAAGCAGTGTACACGCAGTAGTACGCCAGCGTGTCGCGGCGAG

TACCTGGATGTNGTCAGAGACAGGGAGAATTGNATTGGNCGGCCAGAAAT

GATTAGTGGTCGCTATGTTTCAAGAGANGCAATCCAACCAGAGATGTGTT

GGAAAAAGCTCTAAGTTCCTTCCATTCCTTGAAAGAAAGGTCCGAAGGGT

CCTAAGAAGGCAAAGGGAACCCTTTCTGAGGAACCAATTCCTGGATCCTC

AGAAGCAAGCCAAGTTTAAATTCCGGAAATTGATTAGTGGTTCCAGGCCA

TGTTAGGTGTAAAAATCCATAGGGCATTCCCATAATTTTTTAATTTATAT

TATGTGTTATAAGGGAAATTTCTTTGGGTANAGTTTATAGCTTACAGGAA

TGTTGAAAAAAATTCTAATGTTAAGAATTAATTAATTTTGATTGAATTTT

TCCTACCAAAATAAACAAGGGAAATGGTTCAACATTGGTTACCCCTTGGC

CCCCTTGGGGCAGGGGCCCGGTCCGGAAGTGGGGCGTTACCTTATCCCTT

GGTACCGGTCCTTTGGAAGGCCGGTTACCTGGCATTGGGTTACCCCCCAA

GTTTGTTGGATTCCCCCCTTTTATAAGTTTGGAAGGGGGCGTTCACAATT

ATGGGCGAGCCGGCCTTCTTGGGGCGGTTACACTTNCCCAATTGGGGTTC

CCATCAAAGCNTTNGTTTTCCCTTTGGTTTGGTTGGCAACAACATTNTGG

TTTA

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q4_Kubota_Hp35

_01_P22_352.F.esd 1579 MegaBACE GAATTGNGGAAGCCAAAAAGCCAGTGTTCANGNAGGCAAGTAGTAACAGG

CCCTTCGAAAGCGGGACCGGCCAGTGACATGGTGATACCTTTGAACGTCT

GTGGCAGCGTCGAGAAACGCCTCAANTANCCNTGNTCCTTCGATTNNAAG

TGTCGTGGGGTCCTCCACGAGAACACTGTGAATGTGGTGTTNACCCGAGA

TCTNGGACCAGGGGGACCANATTTGTTTGCCCCCTGTGGAGGTTTATAAC

CAAAAGGGGCACCACAGAGCCGATATACCGCGGGATTCTTTGCAATTCTT

AATTAAANGCAAACGCAAGACAAACACGGAATTTAACACATTTAGGGATG

TGAATTTGCNGGAATTAAAAGGTTTGGGTTAAAGGGGGNTTGTTAAGCCN

GAGAANCACGNGACAAGGTTTGTGAAAACCTTTGGGATTCCCCCNTNNTA

CCCGTGCAACAANGATTGGCNCTTTTATAACCACAANNGCAAATTACAGC

TTTTCGGTTTTATGGNAGGCCTANACCAAGGAACACANTTACCCTGTNGC

CANCAAAGACACTATTCCCAAAGNGGGAACCCCTTCCNNGAACAGTGAAC

AGACACAGGCCTTTGGCATNCGGGNTAACACCCCTATCAGGTGACCTCAG

CGTGAAACACTGCTCAAAAGTGGAGCCTCATTTATGGGTGNGAGGCAGAA

GNTTTACACGCTATTTAATACCNGTATGAGGAATNACCGGAGAGTATCGA

TNTTTTTTGTGAAAAACGTGCCGTGTGTTTTAAACANTTTNGTGGGCAAT

TTTAGGGAGTGGTATAAACGCCGGCCNAGGACAAGNAACTNAATTAATAA

TCAGATGGGGTATTANAAAGACNAGCNCAGNTATTAAAATATATAAAAGG

GTTATGGAGAACAGTAGGAGGGNGGGGTTTTAACACAAAGTTGTATTTTG

NGGGGACAAGGACAGGAACGAAAGTTTTATGGAGGGAGGAACGAGTGTTT

TTCAAAAAATTGATACCAACATTTATAGTGGAGGTGNAACCNAAATGTTT

AAAAGGGGCGNTTTNTAGGAGTATTAGATATGTTAACCCATTGATTGATG

GTATTNGGTATTTAGGNGACAAAGTAAGATAGTATTTATGAAGGATTGAT

ATAGAAATTTAAAGCCCACAGGAGGATGATCACCAAACGAANGNGAAAAA

ANTTTTTATAAACGACCAAAAAGCAAANGAGCAANAATGATAAAGTGAAA

ATATTTTAACACAGTGAGGACAAATTGAGCACCCAACCAAATNGGAGTAG

AGTGAACAATGGGCCCAAACTTTCTACAAGAAACAGANGGGAGGATTTTG

GGATTANAAACAACAGCANAGGGAGCCGCCATTATATAGGGTATAGANGA

TGGTATTTNGNGGCGCCCTNTAAAAANAAATTTGTAGGANGAAAGAGGTA

TTTTTGGAGAAAAAGGAGGTCTTATTATAAAAGAAGTATTCACACNAAAA

CGGAAGAGTTTTTTTTTAGCAAACAGNGTGATAAAGTAGNGGAACNGAGA

TACTATATTAAGTAGGCNGNAGAANCNGA

>E:\AnalyzedData/50425_4_2_Ql_Kubota_Hp35_01Run01_Cp312_MDl/Q4_Kubota_Hp35

_01_P24_384.F.esd 817 MegaBACE

GGCCCGNCAGCAGTGTGTCACAACCGCAGATGAACCTGCCCGTAATCCTG

ACTGCACTATCATGAGGCAGCAGTAGGATCATGCAGAGCCTGCATGTTAN

CTACTATGGATACAATCATATANCTATGAAGTACAGTCCTGCTTAGACTA

NTACGTGACGTTGATATAAGGNTATCAGATCTGCCTAAAGACTGAATGCG

GTATAGCCTAATTACTCGAGTAAACATTTCCAGATANNTGTTAGTGATGG

ATAGCTGGAAAGTGACTACCCTGGACAAAAGGAGGAACCCAACGCGGATC

CGTGCAAATCTNTGTCCTATAAGTCGTGTTCGAGGGAGGTTATAACCCNG

CGTAATAGGACGTAATTATTAATCTANTATCCTTTCGTTTGGTATACATA

TTGTGTCATTCTCCTTTGTGGGNATACAAGTCATCGATCATAGTGAAATA

AATATNTGATGCTGAATAAGAATTGTGTATTTGTTAGAAACGAGGTCATC

TTGTCTCGAAATCTAACTAATATAGAGGACTGCAAGGATAATCTAACGTA

GTAATAGAACATAGAATCGTACAAGCTAGCCAATCAGGAGATAAATTAGT

ACTTCAAAAGCATAACATACTGAACGGTATTAATAATATCGATACTTAAT

CTNCATANTCTCTGCATTACTTAACCAAACATAAATTCATTGCTNGACTA

AGATATAACGATTTCTTATATCTCGACGGAAATACATCGTTTTCCATCAC CTANAAGTTTTTTTGCNTATNCCCGNCNTTACGTACCCCTGGCTGGCCTG GCACGAAGGCGTACTTC

A number of aspects of the invention have been described. Nevertheless, it will be understood that various modifications may be made without departing from the spirit and scope of the invention. Accordingly, other aspects are within the scope of the following claims.

Claims

WHAT IS CLAIMED IS:

1. An isolated or recombinant nucleic acid sequence comprising

(i) a nucleic acid sequence of at least 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%. 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more sequence identity to a nucleic acid sequence as set forth in Appendix A, wherein the nucleic acid can be used to detect viral infection in Penaeus sp. (shrimp), or, reduce the effect of viral infection, wherein optionally the viral infection comprises white spot syndrome virus;

(ii) a nucleic acid sequence that hybridizes under stringent conditions to a nucleic acid comprising a nucleic acid sequence as set forth in Appendix A, wherein the nucleic acid can be used to detect viral infection in Penaeus sp. (shrimp), or, reduce the effect of viral infection, wherein optionally the viral infection comprises white spot syndrome virus, and the stringent conditions include a wash step comprising a wash in 0.2X SSC at a temperature of about 65⁰C for about 15 minutes, wherein optionally the nucleic acid is at least about 20, 30, 40, 50, 60, 75, 100, 150, 200, 300, 400, 500, 600, 700, 800, 900, 1000 or more residues in length or the full length of a gene or transcript; and optionally the sequence comparison algorithm is a BLAST version 2.2.2 algorithm where a filtering setting is set to blastall -p blastp -d "nr pataa" -F F, and all other options are set to default.

(iii) a nucleic acid sequence complementary to (i) or (ii).

2. The isolated or recombinant nucleic acid sequence of claim 2, wherein said sequence has at least 95% identity.

3. Th isolated or recombinant e nucleic acid sequence of claim 3, wherein said sequence has 100% identity.

4. A vector comprising a nucleic acid sequence of claim 1.

5. A cell comprising the vector of claim 4 or the nucleic acid of claim 1.

6. A feed or feed supplement comprising a recombinant protein or biologically active fragment thereof encoded by a nucleic acid sequence having at least 65% sequence identity to a shrimp gene or fragment thereof, wherein said shrimp gene is up-regulated during viral infection.

7. The feed or feed supplement of claim 6, wherein the shrimp gene is selected from a group of genes consisting of tetraspanin-2, P-selectin precursor, T-cell activation protein, anti-lipopolysaccharide factor, and heat shock protein STIl.

8. The feed or feed supplement of claim 7, wherein the shrimp gene is contained in clone PvWl 1A5, clone PvW4E03₅ clone PvW5G04, clone PvHBl 1, or clone PvWIODOβ.

9. The feed or feed supplement of claim 6, wherein said protein or biologically active fragment thereof is encoded by a nucleic acid listed in Appendix A2.

10. The feed or feed supplement of claim 6, wherein said protein is contained within a cell or provided as a disrupted cell.

11. A feed or feed supplement comprising a nucleic acid sequence of at least 65% sequence identity to a shrimp gene or biologically active portion thereof in a set of genes up- regulated by infection with a virus.

12. The feed or feed supplement of claim 11, wherein said nucleic acid sequence is a nucleic acid sequence listed in Appendix A2.

13. The feed or feed supplement of claim 11, wherein the shrimp gene is selected from a group of shrimp genes consisting of tetraspanin-2, P-selectin precursor, T-cell activation protein, anti-lipopolysaccharide factor, and heat shock protein STIl.

14. The feed or feed supplement of claim 11 , wherein the shrimp gene is contained in clone PvWl 1A5, clone PvW4E03, clone PvW5G04, clone PvHBl 1, or clone PvW10D06.

15. The feed or feed supplement of claim 11, wherein said nucleic acid sequence is contained within a cell or provided as a disrupted cell.

16. The feed or feed supplement of claim 11 , wherein said nucleic acid sequence is produced synthetically.

17. A feed or feed supplement comprising a recombinant protein or biologically active fragment thereof encoded by a nucleic acid sequence with at least 65% or sequence identity to a nucleic acid sequence found in the set of shrimp genes down-regulated with viral infection.

18. The feed or feed supplement of claim 17, wherein the protein is gamm'a- interferon inducible lysosomal thiol reductase precursor, interleukin enhancer-binding factor 3, NF-kappaB essential modulator, chitinase, prophenoloxidase-activating proteinase 2, O- sialoglycoprotein endopeptidase, ubiquitin, or lysozyme.

19. The feed additive or supplement of claim 17, wherein the protein is encoded by a nucleic acid sequence selected from the group of shrimp nucleic acid sequence contained in clonePvHlA02, clone PvW8B06, clone PvW8E09, clone PvW9El l, clone PvW4F07, clone PvHCOo₅ clone PvW04C06, and clone PvW10F4.

20. The feed or feed supplement of claim 17, wherein said protein or biologically active fragment thereof is encoded by a nucleic acid sequence listed in Appendix Al .

21. The feed or feed supplement of claim 17, wherein said protein is provided within a cell or provided as a disrupted cell.

22. A feed or feed supplement comprising a nucleic acid sequence with at least 65% sequence identity to a shrimp gene or biologically active fragment thereof, wherein said nucleic acid sequence encodes a shrimp gene down-regulated at or during viral infection.

23. The feed or feed supplement of claim 22, wherein the nucleic acid sequence is one listed in Appendix A.

24. The feed or feed supplement of claim 22, wherein the shrimp gene is gamma- interferon inducible lysosomal thiol reductase precursor, interleukin enhancer-binding factor 3, NF-kappaB essential modulator, chitinase, prophenoloxidase-activating proteinase 2, O- sialoglycoprotein endopeptidase, ubiquitin, and lysozyme.

25. The feed or feed supplement of claim 22, wherein the nucleic acid sequence is contained in clonePvHlA02, clone PvW8B06, clone PvW8E09, clone PvW9El 1, clone PvW4F07, clone PvHCOβ, clone PvW04C06, and clone PvWl 0F4.

26. The feed or feed supplement of claim 22, wherein said nucleic acid sequence is provided within a cell or provided as a disrupted cell.

27. The feed or feed supplement of claim 22, wherein said nucleic acid sequence is produced synthetically.

28. A therapeutic comprising an isolated or recombinant protein or biologically active portion thereof

(i) having at least 65% sequence identity to a shrimp protein encoded by a shrimp gene up-regulated during viral infection; or,

(ii) encoded by a nucleic acid comprising a sequence as set forth in claim 1

29. The therapeutic of claim 28, wherein the shrimp gene is a nucleic acid sequence listed in Appendix A2.

30. The therapeutic of claim 28, wherein the shrimp gene is tetraspanin-2, P- selectin precursor, T-cell activation protein, anti-lipopolysaccharide factor, and heat shock protein STIl.

31. The therapeutic of claim 28, wherein the shrimp gene is contained in clone PvWl 1A5, clone PvW4E03, clone PvW5G04, clone PvHBl 1, and clone PvW10D06.

32. The therapeutic of claim 28, wherein said protein or biologically active portion thereof is delivered orally.

33. The therapeutic of claim 28, wherein said protein or biologically active portion thereof is delivered by immersion.

34. The therapeutic of claim 28, wherein said protein or biologically active portion thereof is delivered by injection.

35. A therapeutic comprising a nucleic acid sequence of at least 65% or greater sequence identity to a shrimp gene or biologically active fragment thereof that is up-regulated by infection with a virus.

36. The therapeutic of claim 35, wherein the shrimp gene is selected from those listed in Appendix A2.

37. The therapeutic of claim 35, wherein the shrimp gene is tetraspanin-2, P- selectin precursor, T-cell activation protein, anti-lipopolysaccharide factor, and heat shock protein STIl.

38. The therapeutic of claim 35, wherein the shrimp gene is contained in clone PvWl 1 A5, clone PvW4E03, clone PvW5G04, clone PvHBl 1, and clone PvW10D06.

39. The therapeutic of claim 35, wherein said nucleic acid sequence is delivered orally.

40. The therapeutic of claim 35, wherein said nucleic acid sequence is delivered by immersion.

41. The therapeutic of claim 35, wherein said nucleic acid sequence is delivered by injection.

42. A therapeutic comprising a recombinant protein or biologically active fragment thereof encoded by a nucleic acid sequence with at least 65% sequence identity to a shrimp protein down-regulated by infection with a virus.

43. The therapeutic of claim 42, wherein said protein or biologically. active fragement thereof is encoded by a nucleic acid sequence listed in Appendix Al.

44. The therapeutic of claim 42, wherein the protein or biologically active fragment thereof is gamma-interferon inducible lysosomal thiol reductase precursor, interleukin enhancer-binding factor 3, NF-kappaB essential modulator, chitinase, prophenoloxidase- activating proteinase 2, O-sialoglycoprotein endopeptidase, ubiquitin, or lysozyme.

45. The therapeutic of claim 42, wherein the protein or biologically active fragment thereof is encoded by a nucleic acid sequence contained in clonePvHlA02, clone PvW8B06, clone PvW8E09, clone PvW9El l, clone PvW4F07, clone PvHC06, clone PvW04C06, or clone PvWl 0F4.

46. The therapeutic of claim 42, wherein said protein or biologically active fragment thereof is delivered orally.

47. The therapeutic of claim 42, wherein the protein or biologically active fragment thereof is delivered by immersion.

48. The therapeutic of claim 42, wherein the protein or biologically active fragment thereof is delivered by injection.

49. A therapeutic comprising a nucleic acid sequence of at least 65% sequence identity to a shrimp gene or faction thereof down-regulated by infection with a virus.

50. The therapeutic of claim 49, wherein said nucleic acid sequence is one listed in Appendix A.

51. The therapeutic of claim 49, wherein said nucleic acid sequence is gamma- interferon inducible lysosomal thiol reductase precursor, interleukin enhancer-binding factor 3, NF-kappaB essential modulator, chitinase, prophenoloxidase-activating proteinase 2, O- sialoglycoprotein endopeptidase, ubiquitin, and lysozyme.

52. The therapeutic of claim 49, wherein the nucleic acid sequence is contained in clonePvHl A02, clone PvW8B06, clone PvW8E09, clone PvW9El 1, clone PvW4F07, clone PvHC06, clone PvW04C06, or clone PvWl 0F4.

53. The therapeutic of claim 49, wherein said nucleic acid sequence is delivered orally.

54. The therapeutic of claim 49, wherein said nucleic acid sequence is delivered by immersion.

55. The therapeutic of claim 49, wherein said nucleic acid sequence is delivered by injection.

56. A screening method for identifying a substrate of a protein that is up-regulated following infection by a pathogen, comprising:

(a) providing a polypeptide of the invention; or a polypeptide encoded by a nucleic acid of the invention;

(b) providing a test substrate; and

(c) contacting the polypeptide of step (a) with the test substrate of step (b) and detecting a decrease in the amount of substrate or an increase in the amount of reaction product, wherein a decrease in the amount of the substrate or an increase in the amount of a reaction product identifies the test substrate as the polypeptide substrate.

57. The screening method of claim 56, wherein the protein is encoded by a nucleic acid sequence selected from Appendix A, or comprising a sequence as set forth in claim 1.

58. The screening method of claim 56, wherein the protein is tetraspanin-2, P- selectin precursor, T-cell activation protein, anti-lipopolysaccharide factor, or heat shock protein STIl.

59. The screening method of claim 56, wherein the protein is encoded by the nucleic acid seqeunce contained in clone PvWl 1 A5, clone PvW4E03, clone PvW5G04, clone PvHBl 1, or clone PvWl 0D06.

60. A screening method cfor identifying a substrate of a protein that is down- regulated following infection by a pathogen, comprising:

(b) providing a test substrate; and

61. The screening method of claim 6O₃ wherein the protein is encoded by a nucleic acid sequenced selected from Appendix A, or comprising a sequence as set forth in claim 1.

62. The screening method of claim 60, wherein the protein is gamma-interferon inducible lysosomal thiol reductase precursor, interleukin enhancer- binding factor 3, NF- kappaB essential modulator, chitinase, prophenoloxidase-activating proteinase 2, O- sialoglycoprotein endopeptidase, ubiquitin, or lysozyme.

63. The screening method of claim 60, wherein the protein is encoded by the nucleic acid sequence contained in clone PvHl A02, clone PvW8B06, clone PvW8E09, clone PvW9El 1, clone PvW4F07, clone PvHC06, clone PvW04C06, or clone PvWl 0F4.

64. A diagnostic kit for evaluation of infection in crustaceans in aquaculture and food processing comprising at least one differentially expressed gene as a nucleic acid or recombinant polypeptide, wherein the gene can be used to identify a crustacean as being infected or non-infected with the pathogen of interest; and optionally instructions for use of the kit.

65. The diagnostic kit of claim 64, wherein the gene is one selected from Appendix A, or comprising a sequence as set forth in claim 1.

66. A method for treating or preventing infection by a pathogen in an aquatic animal, comprising providing at least one differentially expressed gene as a nucleic acid or recombinant polypeptide, wherein the gene is up-regulated with infection by the pathogen.

67. The method of claim 66, wherein the gene is one selected from Appendix A2.

68. The method of claim 66, wherein the gene is tetraspanin-2, P-selectin precursor, T-cell activation protein, anti-lipopolysaccharide factor, or heat shock protein STIl.

69. The method of claim 66, wherein the gene is contained in clone PvWl 1A5, clone PvW4E03, clone PvW5G04, clone PvHBl 1, or clone PvW10D06.

70. A method for treating or preventing infection by a pathogen in an aquatic animal, comprising providing at least one differentially expressed gene as a nucleic acid or recombinant polypeptide, wherein the gene is down-regulated with infection by the pathogen.

71. The method of claim 70, wherein the gene is one selected from Appendix A.

72. The method of claim 70, wherein the gene is gamma-interferon inducible lysosomal thiol reductase precursor, interleukin enhancer-binding factor 3, NF-kappaB essential modulator, chitinase, prophenoloxidase-activating proteinase 2, O-sialoglycoprotein endopeptidase, ubiquitin, or lysozyme.

73. The method of claim 70, wherein the gene is the nucleic acid sequence contained in clonePvHl A02, clone PvW8B06, clone PvW8E09, clone PvW9El 1, clone PvW4F07, clone PvHC06, clone PvW04C06, or clone PvWl 0F4.

74. The method of claim 66 or 70, wherein the aquatic animal is a crustacean.

75. The method of claim 74, wherein the crustacean is a shrimp.

76. An inhibitory RNA molecule comprising a nucleic acid sequence comprising a nucleic acid sequence or fragment thereof, or complementary sequence, of the nucleic acid sequence listed in Appendix A, or comprising a sequence as set forth in claim 1.

77. A method of preventing or treating infection of an aquatic animal comprising administering the inhibitory RNA molecule of claim 76.

78. A feed or feed supplement comprising the inhibitory RNA molecule of claim 76.

79. The feed or feed supplement of claim 78, wherein the inhibitory RNA molecule is provided in a cell or as a disrupted cell.

80. The feed or feed supplement of claim 79, wherein the cell is a bacterial, yeast, insect, fish, crustacean, or mammalian cell.

81. A method of identifying shrimp lines that are resistant to viral, bacterial, or fungal diseases, comprising identifying at least one differentially expressed gene as a nucleic acid or protein, wherein the gene comprises a nucleic acid sequence provided in Appendix A, or comprising a sequence as set forth in claim 1.

82. A method of screening for a therapeutic that modulates infectious disease in an aquatic animal, comprising identifying a compound which modulates the expression of the gene as a nucleic acid sequence or protein in an infected animal, wherein the gene comprises a nucleic acid sequence listed in Appendix A, or comprising a sequence as set forth in claim 1, whereby the compound is identified as a modulator of infectious disease when said modulation results in the ameriolation or prevention of one or more symptoms caused by the infection.

83. A microsatellite marker comprising at least one nucleic acid sequence listed in Appendix A, or comprising a sequence as set forth in claim 1.

84. A method of developing microsatellite markers employing at least one nucleic acid sequence listed in Appendix A, or comprising a sequence as set forth in claim 1.

85. A microarry comprising at least one nucleic acid sequence or biologically active fragment thereof listed in Appendix A, or comprising a sequence as set forth in claim 1.

86. A biopesticide comprising at least one nucleic acid sequence or biologically active fragment thereof listed in Appendix A, or comprising a sequence as set forth in claim 1.

87. A method for developing a biopesticide employing at least one nucleic acid sequence or biologically active fragment thereof listed in Appendix A, or comprising a sequence as set forth in claim 1.

88. A transgenic plant expressing a protein or biologically active fragment thereof encoded by a nucleic acid sequence disclosed in Appendix A₃ or comprising a sequence as set forth in claim 1.

89. An isolated or recombinant polypeptide encoded by a nucleic acid comprising a sequence as set forth in claim 1.

90. An antisense oligonucleotide comprising a nucleic acid sequence complementary to or capable of hybridizing under stringent conditions to a sequence as set forth in claim 1 , or a subsequence thereof, wherein optionally the antisense oligonucleotide has a length of between about 10 to 50, about 20 to 60, about 30 to 70, about 40 to 80, or about 60 to 100 bases.

91. A method of inhibiting the translation of a viral message in a cell comprising administering to the cell or expressing in the cell an antisense oligonucleotide comprising a nucleic acid sequence complementary to or capable of hybridizing under stringent conditions to a sequence as set forth in claim 1.

92. A double-stranded interference RNA (RNAi) molecule comprising a subsequence of a sequence as set forth in claim 1, wherein optionally the RNAi comprises an siRNA or an miRNA, and optionally the RNAi molecule is about 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 or more duplex nucleotides in length.

93. A method of inhibiting the expression of a viral gene in a cell comprising administering to the cell or expressing in the cell a double-stranded interference RNA (RNAi) molecule as set forth in claim 92.