WO2007080410A1 - Trousse pour échantillonnage de cellules - Google Patents

Trousse pour échantillonnage de cellules Download PDF

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Publication number
WO2007080410A1
WO2007080410A1 PCT/GB2007/000086 GB2007000086W WO2007080410A1 WO 2007080410 A1 WO2007080410 A1 WO 2007080410A1 GB 2007000086 W GB2007000086 W GB 2007000086W WO 2007080410 A1 WO2007080410 A1 WO 2007080410A1
Authority
WO
WIPO (PCT)
Prior art keywords
container
sampling device
cell sampling
kit
cell
Prior art date
Application number
PCT/GB2007/000086
Other languages
English (en)
Inventor
Alexandre Loktionov
Per Gunnar Ernst Aniansson
Hugo Geoffrey Gifford Lywood
Rupert Charles Gifford Lywood
Andrew Humphrey Llewelyn
Original Assignee
Colonix Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Colonix Limited filed Critical Colonix Limited
Publication of WO2007080410A1 publication Critical patent/WO2007080410A1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/0038—Devices for taking faeces samples; Faecal examination devices
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/02—Instruments for taking cell samples or for biopsy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/0096—Casings for storing test samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/26—Inoculator or sampler
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/02—Instruments for taking cell samples or for biopsy
    • A61B10/04—Endoscopic instruments
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
    • A61B2017/00535—Surgical instruments, devices or methods, e.g. tourniquets pneumatically or hydraulically operated
    • A61B2017/00557—Surgical instruments, devices or methods, e.g. tourniquets pneumatically or hydraulically operated inflatable
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B50/00—Containers, covers, furniture or holders specially adapted for surgical or diagnostic appliances or instruments, e.g. sterile covers
    • A61B2050/005—Containers, covers, furniture or holders specially adapted for surgical or diagnostic appliances or instruments, e.g. sterile covers with a lid or cover
    • A61B2050/0062—Containers, covers, furniture or holders specially adapted for surgical or diagnostic appliances or instruments, e.g. sterile covers with a lid or cover closable by a combination of rotation and translation
    • A61B2050/0064—Containers, covers, furniture or holders specially adapted for surgical or diagnostic appliances or instruments, e.g. sterile covers with a lid or cover closable by a combination of rotation and translation by screwing

Definitions

  • the invention relates to a cell sampling kit comprising a cell sampling device and a container and to methods of cell sampling comprising the kit.
  • the invention is particularly, but not exclusively, related to cell sampling kits in the field of biological or medical experimental, analytical or diagnostic assays and disease screening and monitoring.
  • a container having first engagement means configured to be attached to a second container.
  • the container additionally comprises second engagement means configured to attach a closure device to the container.
  • the second container may comprise any suitable container having corresponding features to enable the container of the invention to be attached to the second container.
  • the first engagement means configured to be attached to a second container comprise a first threaded portion present on an inner surface of the container (e.g. an internal thread) .
  • the design of the container of the invention should enable the container of the invention to be screwed to the second container.
  • the cylindrical neck of the container is wider than the body of the container.
  • This embodiment has the advantage that the body of the container may remain of a standard size for any given container and only the size of the cylindrical neck is widened to allow the cylindrical neck of the container of the invention to engage with a threaded portion on the cylindrical neck of a second container.
  • the first engagement means configured to be attached to a second container comprise a compression or bayonet-type fitting which engages with corresponding features on the second container.
  • the second container is a cell sampling device.
  • a kit comprising a cell sampling device and a container having first engagement means configured to be attached to the cell sampling device.
  • a kit comprising a cell sampling device and a container, wherein the cell sampling device and the container each comprise a complimentary threaded portion configured for attachment of the container to the cell sampling device.
  • This embodiment provides the advantage that the container may easily be attached to the cell sampling device and removed after use. Attachment may be effected simply by screwing the container of the invention onto the cell sampling device. Removal may likewise be effected by unscrewing the container from the cell sampling device.
  • references to “container” and “second container” include references to any container suitable for holding a substance (e.g. a solid or a liquid) .
  • the container may generally be suitable for conducting a reaction, assay, screen or other chemical, biochemical or medical experimental or analytical procedure.
  • the container may generally have an elongate body and/or may be transparent to enable analysis to be performed easily and rapidly.
  • the container typically includes a bottle, tube, flask or vial.
  • the container may generally have a square, rectangular or cylindrical body for containing a substance.
  • the container may also generally have a cylindrical neck for attachment of a closure device to the container.
  • the container may also generally have a mouth or an opening into which a substance may be inserted into the container.
  • the container may also generally have a flat, rounded or tapered base.
  • the container provides a modification to existing containers commonly available on the market, such as for example, SterilinTM tubes (Barloworld Scientific Ltd. , Beacon Road, Stone, Staffordshire, United Kingdom. ST15 OSA) , EppendorfTM tubes (Eppendorf AG, Barkhausenweg 1 , 22339 Hamburg, Germany) , FalconTM tubes (BD, 1 Becton Drive, Franklin Lakes, NJ USA 07417), DuranTM bottles (Schott UK Ltd.
  • SterilinTM tubes Barloworld Scientific Ltd. , Beacon Road, Stone, Staffordshire, United Kingdom. ST15 OSA
  • EppendorfTM tubes Eppendorf AG, Barkhausenweg 1 , 22339 Hamburg, Germany
  • FalconTM tubes BD, 1 Becton Drive, Franklin Lakes, NJ USA 07417)
  • DuranTM bottles Schott UK Ltd.
  • the container may typically be fabricated from glass, PyrexTM or a plastics material (e.g. polypropylene, polyethylene or polystyrene) .
  • the container is constructed from a plastics material (e.g. polypropylene, polyethylene or polystyrene) .
  • This embodiment has the advantage of providing strength to the container, while being more likely to retain the contents when subjected to impacting stress (e.g. if the container is dropped or knocked during transport) .
  • the threaded portion is present on an inner surface of the container.
  • the threaded portion present on an inner surface of the container engages with the threaded portion on the cell sampling device commonly found on the exterior surfaces of containers for usual engagement with a threaded cap or lid.
  • the contents of the cell sampling device may therefore simply be transferred to the container without loss or leakage.
  • the threaded portion is present on an inner surface of the cylindrical neck of the container.
  • the threaded portion may at least partially surround the circumference of an inner surface of the cylindrical neck of the container.
  • the threaded portion entirely surrounds the circumference of an inner surface of the cylindrical neck of the container.
  • the embodiment wherein the threaded portion partially surrounds the inner surface circumference of the cylindrical neck provides the advantage that when transferring a sample into the container or when removing a sample from the container, the contents are channelled into and out of the container more effectively than when the threaded portion entirely surrounds the inner surface circumference of the cylindrical neck.
  • the container and/or cell sampling device comprise engagement means configured to attach a closure device thereto.
  • the engagement means may typically comprise a second threaded portion on an outer surface of the container (i.e. the container will have a first threaded portion present on an inner surface (e.g. an internal thread for attachment to the cell sampling device) and a threaded portion present on an outer surface (e.g. an external thread for attachment to the container)) .
  • the closure device may engage with a single threaded portion present on the outer surface of the cell sampling device (e.g. an external thread for attachment to both the container and the closure device) .
  • the kit additionally comprises a closure device.
  • the closure device is a cap or lid having a threaded portion on an inner surface.
  • the closure device is a snap-fit cap, lid, stopper or bung.
  • the closure device is a snap-fit cap, lid, stopper or bung and the engagement means present on the container and/or cell sampling device comprise one or more protrusions configured to engage with the lid, stopper or bung in a snap-fit arrangement.
  • a threaded portion may be present on the inner and/or outer surfaces of the container and the inner and/or outer surfaces of the cell sampling device.
  • a first threaded portion is present on the inner surface of the container (e.g. an internal thread) and a second threaded portion is present on the outer surface of the cell sampling device (e.g. an external thread) .
  • This first embodiment provides an arrangement wherein the container and cell sampling device may be attached to each other via the first and second threaded portions.
  • a closure device for the container may be a stopper or bung or otherwise a cap or lid having a threaded portion on an outer surface (e.g. an external thread) for sealing the container having the internal thread.
  • a closure device for the cell sampling device may be a cap or lid having a threaded portion on an inner surface (e.g.
  • a first threaded portion is present on the inner surface of the container (e.g. an internal thread)
  • a second threaded portion is present on the outer surface of the container (e.g. an external thread)
  • a third threaded portion is present on the outer surface of the cell sampling device (e.g. an external thread)
  • the container comprises both an internal and external threaded portion.
  • a closure device for the container and cell sampling device may be a cap or lid having a threaded portion on an inner surface (e.g. an internal thread) for sealing both the cell sampling device and container each of which have an external thread.
  • a first threaded portion is present on the outer surface of the container (e.g. an external thread) and a second threaded portion is present on the inner surface of the cell sampling device (e.g. an internal thread) .
  • This third embodiment provides an arrangement wherein the container and cell sampling device may be attached to each other via the first and second threaded portions.
  • a closure device for the cell sampling device may be a stopper or bung or otherwise a cap or lid having a threaded portion on an outer surface (e.g. an external thread) for sealing the cell sampling device having the internal thread.
  • a closure device for the container may be a cap or lid having a threaded portion on an inner surface (e.g. an internal thread) for sealing the container having the external thread.
  • the container and cell sampling device each comprise a single threaded portion
  • the single threaded portion may serve to engage both with the container or cell sampling device and a closure device.
  • the container comprises a volume of liquid.
  • a closure device with additional sealing means may be required to create an efficient seal without loss of material during transfer and storage.
  • the threaded portion may have a reservoir attached thereto.
  • the reservoir contains a liquid.
  • the reservoir is a blister packet.
  • This embodiment provides the advantage that the action of screwing the container of the invention to the cell sampling device breaks the reservoir (e.g. blister packet) and therefore releases the contents into the cell sampling device.
  • This embodiment therefore enhances the process of suspending cells (or other collected solid substance or particulate material) present within the cell sampling device. For example, the cells will be suspended in the liquid and the entire contents can be transferred to the container of the invention without loss of material.
  • the liquid is a buffer such as an assay buffer, cell suspending buffer or cell lysis buffer, or a cell preserving solution.
  • the liquid is a buffer, such as a cell suspending buffer.
  • the buffer is a cell-lysis buffer which has the advantage of providing a key step prior to DNA extraction.
  • the cell-lysis buffer may additionally comprise a proteolytic enzyme. This embodiment provides the advantage of being useful in nucleic acid purification.
  • the cell-lysis buffer may additionally comprise immunomagnetic beads. This embodiment provides the advantage of allowing the efficient capture of nucleic acids.
  • the buffer is a cell-preserving solution which has the advantage of allowing enhanced cytological, biochemical and imniunohistochemical analyses on the resultant cell sample.
  • the cell-preserving solution is supplemented with one or more cell culture components (e.g. nutrients and antibiotics) .
  • the container comprises graduated marks or grooves. This embodiment provides the advantage of indicating the volume of the contents of the container to the user.
  • the cell sampling device comprises a flexible membrane having a cell sampling surface.
  • the cell sampling device comprises an insertion member having a distal, insertion end, a proximal end and a closable interior cavity, a flexible membrane having an outer, cell sampling surface and an inner surface, wherein the membrane is sealingly attached to the distal, insertion end of the insertion member and held within the interior cavity, such that, in use, pressurisation of the interior cavity to at least a first elevated pressure causes the membrane to emit from the distal end of the insertion member to make contact with the cells and pressurisation of the interior cavity to a second reduced pressure causes the membrane to invert and return to the interior cavity of the insertion member.
  • the cell sampling device is a colorectal cell sampling device.
  • the cell sampling device is a colorectal cell sampling device as defined in WO 2006/003447 Al (Colonix Limited) - the features and methods of which are herein incorporated by reference.
  • the flexible membrane is expandable and is constructed from an elastic material.
  • the flexible membrane is constructed from a nitrile, latex or rubber based substance.
  • the closable interior cavity of the insertion member is closed.
  • the cell sampling device further comprises means for pressurisation of the interior cavity, wherein the means are attached to the proximal end of the insertion member.
  • the means for pressurisation of the interior cavity are attached to the cell sampling device via a valve (e.g. a self -sealing valve) present at the proximal end of the insertion member.
  • the means for pressurisation of the interior cavity may comprise any means suitable for applying a fluid (e.g. liquid or gas) to the flexible membrane.
  • a fluid e.g. liquid or gas
  • the means for pressurisation of the interior cavity comprise a source of compressed air, a syringe or a pump (e.g. bulb).
  • the means for pressurisation of the interior cavity comprise a source of compressed air which comprises a mechanical device capable of delivering a pre-defined quantity of a first elevated pressure and a second reduced pressure to the cell sampling device.
  • a source of compressed air which comprises a mechanical device capable of delivering a pre-defined quantity of a first elevated pressure and a second reduced pressure to the cell sampling device.
  • This embodiment has the advantage of accurately regulating the pressure inside the insertion member and the mechanical device has the advantage of being re-used with an indefinite number of disposable colorectal cell sampling devices.
  • the means for pressurisation of the interior cavity comprise a syringe. The use of a syringe, allows for both simple operation, and for a fixed volume of air to be pumped into the flexible membrane (preferably at least a ten fold increase in the volume of air present in the flexible membrane) .
  • the plunger of said syringe could initially be set at the 70-90ml mark.
  • a pre-defined quantity of a first elevated pressure could therefore be applied by pushing the plunger to its maximum extent (e.g. to the 0ml mark) which would fill the flexible membrane with an air volume of 70-90ml.
  • a pre-defined quantity of a second reduced pressure could then subsequently be applied by pulling the plunger of the syringe back to its maximum extent (e.g. to the 100ml mark) which would draw the membrane into the interior cavity of the insertion member.
  • the syringe would be supplied with one or more retention features (e.g. snap locations) to mark the plunger positions of the syringe at each stage during use (e.g. one position prior to insertion, one during insertion and one after withdrawal) .
  • retention features e.g. snap locations
  • the advantage of the means for pressurisation of the interior cavity being a syringe is that the colorectal cell sampling device may be adapted to fit onto commonly available and disposable laboratory and hospital equipment.
  • the surface area of the outer, cell sampling surface of said flexible membrane is reproducibly controllable. This allows for a fixed surface area to be brought into contact with the mucosal surface being sampled, thereby providing a quantifiable collection of exfoliated cells which is correlated with the amount present on the surface of the colorectal mucosa.
  • the surface area is controlled by the means for pressurisation of the interior cavity. This allows for a fixed surface area to be brought into contact with the mucosal surface being sampled.
  • the insertion member is adapted to engage with a rectal access tube.
  • This embodiment has the advantage of allowing a rectal access tube and an obturator, such as an olive shaped obturator (a conjoined rectal access tube and obturator is commonly known as a proctoscope) to be inserted first to open the rectal cavity followed by withdrawal of the obturator prior to insertion of the sampling device of the invention.
  • the sampling device could then remain held in position with the rectal access tube for whatever period of time was required to obtain a sample.
  • the obturator would then be replaced once the sampling device is removed and the obturator and rectal access tube would be withdrawn together.
  • the insertion member is configured to allow self- insertion.
  • the insertion member is inserted together with a rectal access tube and therefore eliminates the need for an obturator (e.g. the insertion member has a rounded distal, insertion end) .
  • This embodiment provides the advantage that the sampling device may be self -administered, ⁇ for example, patients will be easily able to sample exfoliated cells from their rectal mucosa.
  • the insertion member and rectal access tube are inserted and removed together and only separated upon removal.
  • the flexible membrane forms a receptacle when held within the interior cavity of said insertion member, such that fluid from the container or other vessel may be added.
  • This embodiment of the invention would allow for reagents to be added to the sampling device after a sample has been obtained without the need to transfer the sample to a separate receptacle, thereby losing some of the material from the sample.
  • the interior cavity of the insertion member is provided with adhesion means.
  • This embodiment of the invention has the effect of drawing the flexible membrane towards the walls of the interior cavity of the insertion member once a sample has been obtained and application of the second reduced pressure has drawn the flexible membrane into the interior cavity of the insertion member.
  • This feature has the advantage of providing a stable receptacle when filled with liquid.
  • the cell sampling device comprises means for pressurisation of the interior cavity
  • said means are preferably detachable from the insertion member. This has the advantage of converting the sampling device into a compact assay vial which may be conveniently transported and stored with many other compact assay vials for subsequent screening reactions.
  • the kit additionally comprises a rectal access tube and optionally an obturator.
  • a rectal access tube provides both for more comfortable insertion of the sampling device, and prevents contact between the sampling device and any surface other than the mucosal surface to be sampled.
  • the use of an obturator in addition to the rectal access tube, may ease the discomfort of inserting the rectal access tube.
  • the kit may additionally comprise a lubricant, such as a lubricating jelly (e.g. K-Y jelly) .
  • a lubricating jelly e.g. K-Y jelly
  • the obturator is disengaged from the rectal access tube after insertion of the conjoined obturator and rectal access tube into the rectal cavity.
  • any of the devices or kits previously described are suitable for sampling exfoliated epithelial tissue (e.g. colonocytes) from the surface of human colorectal mucosa.
  • the threaded portion is present on an outer surface of the distal, insertion end of the cell sampling device.
  • a cell sampling device as defined herein to a container as defined herein, comprising the steps of:
  • a method of transferring exfoliated cells from a colorectal mucosal surface of a human subject to a container comprising the steps of:
  • sampling step (a) may typically comprise the following steps:
  • Figure 1 shows a plan view of a container according to one embodiment of the invention.
  • Figure 2 schematically shows a method of transferring exfoliated cells from a colorectal mucosal surface of a human subject to a container according to the embodiment shown in Figure 1.
  • Figure 3 schematically shows an alternative method of transferring exfoliated cells from a colorectal mucosal surface of a human subject to a container according to the embodiment shown in Figure
  • a container shown generally as 100, has a generally cylindrical and hollow body 110, a rounded base 111 and a cylindrical neck 112 having a wider diameter than the body 110.
  • the container 100 also comprises a first threaded portion 101 configured to be attached to a cell sampling device.
  • the first threaded portion 101 is present around the circumference of the inner surface of the cylindrical neck 112 of the container 100.
  • the container 100 also comprises a second threaded portion 102 for attachment of a closure device to the container 100.
  • the second threaded portion 102 is present around the circumference of the outer surface of the cylindrical neck 112 of the container 100.
  • the closure device 105 will typically have a threaded portion 106 for attachment to the second threaded portion 102 of the container 100.
  • the threaded portion 106 is shown to be present around the circumference of the inner surface of the closure device 105.
  • the kit of the invention may generally comprise the container 100 shown in Figure 1 , the cell sampling device 7 shown in Figures 2A-2F and a closure device 105 shown in Figure 2G.
  • exfoliated cells from a colorectal mucosal surface of a human subject may be sampled in accordance with the procedures described in WO 2006/003447 Al and as shown in Figures 2A-2C.
  • a first, elevated pressure is applied to the cell sampling device 7 (shown in Figure 2A as a syringe) which causes the flexible membrane 4 to inflate and emit from the distal end of the cell sampling device 7 ( Figure 2B) .
  • the inflated flexible membrane 4 then makes contact with the colorectal mucosal surface of a human subject such that exfoliated cells 31 are transferred to the outer surface of the flexible membrane 4 ( Figure 2B) .
  • a second reduced pressure is then applied to the cell sampling device 7 which causes the flexible membrane 4 to invert and return to the interior cavity of the cell sampling device 7 ( Figure 2C) .
  • Assay buffer or cell suspending buffer 103 from a vessel 104 is then introduced into the distal end of the cell sampling device 7 such that contact is made with the exfoliated cells 31 on the outer surface of the flexible membrane 4 ( Figure 2D) .
  • the assay buffer or cell suspending buffer 103 and exfoliated cells 31 will therefore be contained within the inverted flexible membrane 4.
  • the container 100 is then screwed onto the threaded distal end of the cell sampling device 7 by way of engagement of the first threaded portion 101 present on the inner surface of the container 100 and a further threaded portion 701 present on the outer surface of the distal end of the cell sampling device 7 (Figure 2E) .
  • the container 100 and cell sampling device 7 are then agitated to ensure thorough suspension of the exfoliated cells 31 in the assay buffer or cell suspending buffer 103 and the container 100 and cell sampling device 7 are tilted to ensure that the contents 31 and 103 are transferred to the container 100 ( Figure 2F) .
  • the closure device 105 is then attached to the container 100 by way of engagement of the threaded portion 106 on the inner surface of the closure device 105 with the second threaded portion 102 on the outer surface of the container 100 ( Figure 2G) .
  • Figure 3 An alternative embodiment to the method described in Figure 2 is demonstrated by Figure 3.
  • the assay buffer or cell suspending buffer 103 will be contained as a pre-measured amount within the container 100 (as shown in Figure 3D) .
  • Such an arrangement will introduce the assay buffer or cell suspending buffer 103 from the container 100 to the cell sampling device 7 upon attachment and agitation shown in Figures 3E-3F.
  • This embodiment therefore eliminates the need for the step performed in Figure 2D because suspension will occur simultaneously with the step performed in Figures 2E and 3E-3F.
  • a pre-measured amount of assay buffer or cell suspending buffer 103 will be present within a reservoir (e.g. blister packet) present on the first threaded portion 101 of the container 100.
  • a reservoir e.g. blister packet
  • Such an arrangement will release the assay buffer or cell suspending buffer 103 from the reservoir (e.g. blister packet) upon screwing the container 100 to the cell sampling device 7.
  • this eliminates the need for the step performed in Figure 2D because suspension will occur simultaneously with the step performed in Figures 2E and 3E.
  • alternative means to pressurise the cell sampling device may be envisaged other than the use of a syringe 7.
  • compressed air may be administered to the cell sampling device either by a pump or an electronic or mechanical device.
  • the container 100 shown in Figure 1 may be modified to remove the second threaded portion 102 from the outer surface of the container (i.e. the container will have only a single threaded portion 101 on the inner surface of the container 100) .
  • the closure device 105 shown in Figure 2G will also be modified to be either a stopper or bung or otherwise a cap or lid having a threaded portion on an outer surface (e.g. an external thread) for sealing the container 100 having only an internal threaded portion 101.

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Abstract

L'invention concerne une trousse pour échantillonnage de cellules comprenant un dispositif (7) d'échantillonnage de cellules et un récipient (100), le dispositif (7) d'échantillonnage de cellules et le récipient (100) étant chacun pourvus d'une partie filetée (101, 701) configurée pour que le récipient (100) puisse être fixé sur le dispositif (7) d'échantillonnage de cellules. L'invention concerne également des procédés d'échantillonnage de cellules comprenant la trousse. L'invention concerne particulièrement, mais non exclusivement, des trousses pour échantillonnage de cellules utilisées dans le domaine des dosages expérimentaux, analytiques ou diagnostiques, biologiques ou médicaux et du dépistage et de la surveillance de maladies.
PCT/GB2007/000086 2006-01-12 2007-01-12 Trousse pour échantillonnage de cellules WO2007080410A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0600595.3A GB0600595D0 (en) 2006-01-12 2006-01-12 Container
GB0600595.3 2006-01-12

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WO2007080410A1 true WO2007080410A1 (fr) 2007-07-19

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PCT/GB2007/000086 WO2007080410A1 (fr) 2006-01-12 2007-01-12 Trousse pour échantillonnage de cellules

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GB (2) GB0600595D0 (fr)
WO (1) WO2007080410A1 (fr)

Cited By (4)

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US8309343B2 (en) 2008-12-01 2012-11-13 Baxter International Inc. Apparatus and method for processing biological material
WO2014057283A1 (fr) * 2012-10-12 2014-04-17 Origin Sciences Limited Procédé de diagnostic pour cancer colorectal
GB2543728B (en) * 2014-08-12 2019-04-17 Nextgen Jane Inc Medical kit and method for processing a biological sample
US11446011B2 (en) 2016-04-13 2022-09-20 Nextgen Jane, Inc. Sample collection and preservation devices, systems and methods

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115568461B (zh) * 2022-10-25 2024-01-26 马鞍山普梅森医学检验实验室有限公司 一种无菌细胞病理学液体样本储存盒

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