WO2007026049A2 - Procede ameliore de fermentation par reparation de genes de levure - Google Patents

Procede ameliore de fermentation par reparation de genes de levure Download PDF

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Publication number
WO2007026049A2
WO2007026049A2 PCT/FI2006/000292 FI2006000292W WO2007026049A2 WO 2007026049 A2 WO2007026049 A2 WO 2007026049A2 FI 2006000292 W FI2006000292 W FI 2006000292W WO 2007026049 A2 WO2007026049 A2 WO 2007026049A2
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WO
WIPO (PCT)
Prior art keywords
gene
yeast
maltose
strain
sequence
Prior art date
Application number
PCT/FI2006/000292
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English (en)
Other versions
WO2007026049A3 (fr
Inventor
John Londesborough
Virve Vidgren
Laura Ruohonen
Original Assignee
Oy Panimolaboratorio-Bryggerilaboratorium Ab
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Filing date
Publication date
Application filed by Oy Panimolaboratorio-Bryggerilaboratorium Ab filed Critical Oy Panimolaboratorio-Bryggerilaboratorium Ab
Publication of WO2007026049A2 publication Critical patent/WO2007026049A2/fr
Publication of WO2007026049A3 publication Critical patent/WO2007026049A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C12/00—Processes specially adapted for making special kinds of beer
    • C12C12/002—Processes specially adapted for making special kinds of beer using special microorganisms
    • C12C12/004—Genetically modified microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C11/00—Fermentation processes for beer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C11/00—Fermentation processes for beer
    • C12C11/003—Fermentation of beerwort
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C12/00—Processes specially adapted for making special kinds of beer
    • C12C12/002—Processes specially adapted for making special kinds of beer using special microorganisms
    • C12C12/006—Yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un procédé destiné à accroître la vitesse et l'étendue des fermentations. Selon ce procédé dans la fermentation une souche de levure transformée est utilisée avec une molécule d'ADN contenant une séquence nucléotide qui code une partie de la séquence d'acide aminé pour un transporteur d'alpha-glucoside. La transformation convertit l'hôte transformé afin de posséder une capacité accrue pour le transport de maltose ou de maltotriose, ou les deux par rapport à un hôte non transformé. L'invention concerne également un procédé destiné à préparer une souche de levure destinée à la fermentation, une nouvelle souche de levure et une cassette d'intégration d'ADN.
PCT/FI2006/000292 2005-09-01 2006-08-31 Procede ameliore de fermentation par reparation de genes de levure WO2007026049A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20050870A FI20050870L (fi) 2005-09-01 2005-09-01 Parannettu fermentointimenetelmä hiivageenejä korjaamalla
FI20050870 2005-09-01

Publications (2)

Publication Number Publication Date
WO2007026049A2 true WO2007026049A2 (fr) 2007-03-08
WO2007026049A3 WO2007026049A3 (fr) 2007-05-10

Family

ID=35151329

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FI2006/000292 WO2007026049A2 (fr) 2005-09-01 2006-08-31 Procede ameliore de fermentation par reparation de genes de levure

Country Status (2)

Country Link
FI (1) FI20050870L (fr)
WO (1) WO2007026049A2 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2189525A1 (fr) * 2008-09-09 2010-05-26 Suntory Holdings Limited Transporteur alpha-glucosidique hybride
CN101952425B (zh) * 2008-09-09 2012-08-15 三得利控股株式会社 葡萄糖诱导性失活及降解抗性转运蛋白基因及其用途
EP3663392A1 (fr) * 2013-08-07 2020-06-10 Amyris, Inc. Procédés pour stabiliser la production de composés dérivés de l'acétyl-coenzyme a

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1217074A1 (fr) * 2000-12-22 2002-06-26 Universiteit Leiden Intégration d'ADN dans des eukaryotes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1217074A1 (fr) * 2000-12-22 2002-06-26 Universiteit Leiden Intégration d'ADN dans des eukaryotes

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
KOMADA Y ET AL: "Improvement of Maltose Fermentation Efficiency: Constitutive Expression of MAL Genes in Brewing Yeasts" JOURNAL OF THE AMERICAN SOCIETY OF BREWING CHEMISTS, AMERICAN SOCIETY OF BREWING CHEMISTS, ST.PAUL, MN,, US, vol. 53, no. 1, 1995, pages 24-29, XP002996798 ISSN: 0361-0470 cited in the application *
RAUTIO JARI ET AL: "Maltose transport by brewer's yeasts in brewer's wort." JOURNAL OF THE INSTITUTE OF BREWING, vol. 109, no. 3, 2003, pages 251-261, XP001249133 ISSN: 0046-9750 cited in the application *
STEARMAN R ET AL: "YIpDCE1 - an integrating plasmid for dual constitutive expression in yeast" GENE: AN INTERNATIONAL JOURNAL ON GENES AND GENOMES, ELSEVIER, AMSTERDAM, NL, vol. 212, no. 2, 8 June 1998 (1998-06-08), pages 197-202, XP004122921 ISSN: 0378-1119 & DATABASE EMBL 23 June 1998 (1998-06-23), STEARMAN R.: "Integrating expression vector YIpDCE1, complete sequence" Database accession no. EM_SY:AF039102 *
VIDGREN VIRVE ET AL: "Characterization and functional analysis of the MAL and MPH loci for maltose utilization in some ale and lager yeast strains" APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 71, no. 12, December 2005 (2005-12), pages 7846-7857, XP002419699 ISSN: 0099-2240 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2189525A1 (fr) * 2008-09-09 2010-05-26 Suntory Holdings Limited Transporteur alpha-glucosidique hybride
EP2189525A4 (fr) * 2008-09-09 2012-03-28 Suntory Holdings Ltd Transporteur alpha-glucosidique hybride
CN101952425B (zh) * 2008-09-09 2012-08-15 三得利控股株式会社 葡萄糖诱导性失活及降解抗性转运蛋白基因及其用途
US8735092B2 (en) 2008-09-09 2014-05-27 Suntory Holdings Limited Glucose-induced inactivation/degradation-resistant transporter gene and use thereof
EP3663392A1 (fr) * 2013-08-07 2020-06-10 Amyris, Inc. Procédés pour stabiliser la production de composés dérivés de l'acétyl-coenzyme a
US11230716B2 (en) 2013-08-07 2022-01-25 Amyris, Inc. Methods for stabilizing production of acetyl-coenzyme A derived compounds

Also Published As

Publication number Publication date
FI20050870L (fi) 2007-03-02
FI20050870A0 (fi) 2005-09-01
WO2007026049A3 (fr) 2007-05-10

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