WO2006100838A1 - Method for increasing amount of tastant and/or flavor in meat and feedstuff and drinking water for the same - Google Patents

Method for increasing amount of tastant and/or flavor in meat and feedstuff and drinking water for the same Download PDF

Info

Publication number
WO2006100838A1
WO2006100838A1 PCT/JP2006/301828 JP2006301828W WO2006100838A1 WO 2006100838 A1 WO2006100838 A1 WO 2006100838A1 JP 2006301828 W JP2006301828 W JP 2006301828W WO 2006100838 A1 WO2006100838 A1 WO 2006100838A1
Authority
WO
WIPO (PCT)
Prior art keywords
lysine
meat
feed
poultry
flavor
Prior art date
Application number
PCT/JP2006/301828
Other languages
French (fr)
Japanese (ja)
Inventor
Shinobu Fujimura
Yuya Watanabe
Motoni Kadowaki
Original Assignee
Niigata University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Niigata University filed Critical Niigata University
Priority to JP2007509158A priority Critical patent/JP4945762B2/en
Publication of WO2006100838A1 publication Critical patent/WO2006100838A1/en

Links

Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/70Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75Feeding-stuffs specially adapted for particular animals for birds for poultry
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof

Definitions

  • the present invention relates to a method for increasing the amount of flavor components and Z or flavor of meat, and feed and drinking water therefor, and more particularly, a method for increasing the amount of free glutamic acid and Z or flavor in poultry or livestock meat. And feed and drinking water for it.
  • the required amount of arginine is set to 1.10% for 3-6 weeks old and 1.00% for 6-8 weeks old in the American NRC breeding standard (1994 version). This amount is the basis for the arginine requirement.
  • the Japanese breeding standard (2004 version) sets the arginine requirement to 1.17% after 3 weeks of age.
  • the mixed feed generally used in Japan, the United States, etc. is mainly composed of corn, which is an energy source, and soybean meal, which is a plant protein source, and lysine and arginine in the mixed feed using this. The content of is about 1.0 times the NRC requirement.
  • Patent Document 1 A method for increasing the amount of free glutamic acid in meat and a feed for the same have been proposed.
  • Patent Document 1 International Publication No. WO2005Z018315 Pamphlet
  • an object of the present invention is to provide a method for increasing the amount of flavor components and the Z or flavor of meat by adjusting the components of feed or drinking water for poultry or livestock. It is another object of the present invention to provide feed and drinking water that can produce meat that has many flavor components and is excellent in flavor and taste and that can be easily manufactured.
  • the method of increasing the taste component amount and Z or flavor of the meat of the present invention comprises the basic amino acid content. It is characterized by feeding high-powder feed to poultry or livestock.
  • the basic amino acid strength is lysine and Z or arginine.
  • the lysine content is 120 to 200% with respect to the lysine nutrient requirement.
  • the arginine content is 100 to 130% with respect to the arginine nutrient requirement.
  • water containing basic amino acids is supplied to poultry or livestock.
  • the feed of the present invention is a poultry or livestock feed for increasing the amount of taste components and Z or flavor of meat, and contains 120 to 200% of lysine with respect to lysine nutrient requirements. It is characterized by that.
  • the drinking water of the present invention is a poultry or livestock drinking water for increasing the amount of flavor components and Z or flavor of meat, characterized in that a basic amino acid is mixed in water.
  • the poultry or livestock of the present invention is characterized by being fed with a feed containing 120 to 200% of lysine with respect to lysine nutrient requirement.
  • the present invention is characterized by being fed with a feed containing 120 to 200% lysine with respect to lysine nutrient requirement and 100 to 130% arginine with respect to arginine nutrient requirement. .
  • the meat of the present invention is obtained from the above-mentioned poultry or livestock.
  • the amount of free glutamic acid in the meat can be increased.
  • This increase in the amount of free glutamic acid can be identified as an improvement in the taste, and can improve the added value of the meat.
  • the feed of the present invention can be easily produced by adding lysine and Z or arginine in excess of the standard nutritional requirements. By supplying this feed to poultry or livestock, meat is consumed. The amount of free glutamic acid can be increased.
  • the drinking water of the present invention can be easily produced by mixing a basic amino acid in water. By supplying this water to poultry or livestock, the amount of free glutamic acid in the meat can be increased.
  • the poultry or livestock of the present invention is bred with a feed containing lysine and Z or algin exceeding the standard nutritional requirement, and is rich in free glutamic acid.
  • the meat of the present invention is obtained from the above-mentioned poultry or livestock power and contains abundant free glutamic acid.
  • FIG. 1 is a graph showing the relationship between lysine content in feed and free glutamate concentration in meat in Example 1 of the present invention.
  • FIG. 2 is a graph showing the relationship between lysine content in feed and free glutamate concentration in meat in Example 2 of the present invention.
  • FIG. 3 is a drape showing the result of sensory evaluation by a two-point comparison method for meat in Example 3 of the present invention.
  • FIG. 4 is a graph showing sensory evaluation results by a paired comparison method of meat Schiffe in Example 3 of the present invention.
  • FIG. 5 is an explanatory diagram showing the mechanism of glutamate metabolism in vivo according to the present invention.
  • FIG. 6 is a graph showing the relationship between the lysine content in the feed and the glutamate dehydrogenase activity in Example 4 of the present invention.
  • FIG. 7 is a graph showing the relationship between lysine content in feed and glutamine synthetase activity in Example 4 of the present invention.
  • the method for increasing the taste component amount and Z or flavor of meat according to the present invention is to feed a poultry or livestock with a feed having a high basic amino acid content.
  • poultry to which the present invention can be applied include chickens, duck, turkeys, pigeons, and sharks
  • livestock include cattle, pigs, sheep, goats, and horses.
  • the present invention is not limited to this and can be widely applied.
  • the feed used in the present invention is contained in a high cocoon content! /
  • the basic amino acids are lysine and arginine. Lysine and arginine are taken orally and are rewarded by the liver.
  • lysine and arginine may be added to the feed and fed to poultry or livestock.
  • lysine and arginine for addition those generally sold at low cost for addition to feed can be used.
  • the lysine content in the feed is preferably 120 to 200%, more preferably 140 to 170% of the standard lysine nutrient requirement.
  • the content of lysine is less than 120%, an increase in the amount of meat taste components is not observed, which is not preferable.
  • the content of lysine is more than 200%, although the amount of meat-flavoring components is increased, the cost required for the addition of lysine is not preferable.
  • the standard lysine nutrient requirements are based on the breeding standards (1994 version) of the American National Research Council (NRC), Nutrient Requirement of Poultry.
  • the content of arginine in the feed is preferably 100 to 130% of the standard arginine nutrient requirement.
  • the content of arginine is less than 100%, an increase in the amount of meat taste components is not observed, which is not preferable.
  • the content of arginine is more than 130%, although the amount of meat-tasting components is increased, the cost required for adding arginine is not preferable.
  • the standard arginine nutrient requirements are based on the NRC breeding standard (1994 edition).
  • feed the feed of the present invention it is preferable to feed the feed of the present invention from at least 2 days before shipment to immediately before shipment, and even after 10 days before shipment, until immediately before shipment. It is preferable to pay. In this way, by feeding from several days before shipment to just before shipment, the cost of working on feeds with high lysine and Z or arginine content can be reduced, and the flavor component of meat can be increased just before shipment. Can do.
  • the method for increasing the taste component amount and Z or flavor of meat according to the present invention supplies water containing basic amino acids to poultry or livestock.
  • the lysine content in drinking water is 0 It is preferable to be in the range of 1 to 2.0% by mass, and it is more preferable to be in the range of 0.2 to 0.7% by mass. Within this range, it is possible to sufficiently exert glutamic acid, which is a meat taste component, and meat flavor.
  • Water containing basic amino acids may be supplied to poultry or livestock from a poultry or livestock water dispenser.
  • the amount of free glutamic acid in the meat can be increased.
  • This increase in the amount of free glutamic acid can be discriminated as an improvement in the taste, which can improve the added value of the meat and increase the consumption of high quality meat.
  • the umami of meat is enhanced by increasing the amount of glutamic acid, the amount of chemical seasoning used during cooking can be reduced. Therefore, the meat produced by the method of the present invention is consistent with the recent consumption trend to reduce the amount of chemical seasonings used with emphasis on natural ingredients.
  • the method of the present invention can easily increase the taste component and Z or flavor of meat as compared with the method of increasing the taste component by aging and processing of meat after slaughter. Even after aging after slaughter, the amount of glutamic acid is higher than that of the normal rearing group.
  • the feed of the present invention is a poultry or livestock feed for increasing the amount of flavor components and Z or flavor of meat, and contains 120 to 200% of lysine with respect to lysine nutrient requirements. Is. It may also contain 100-130% arginine relative to the arginine nutrient requirements.
  • the feed of the present invention can be easily produced by adding lysine and Z or arginine in excess of the standard nutritional requirements, and by supplying this feed to poultry or livestock, It is possible to increase the amount of free glutamic acid and the flavor of meat, which are the taste components in it.
  • the drinking water of the present invention is poultry or livestock drinking water for increasing the amount of flavor components and Z or flavor of meat, and is obtained by mixing basic amino acids with water.
  • the basic amino acid preferably contains lysine.
  • the content of lysine in drinking water is preferably an amount that allows intake of 0.1 to 2.0% lysine per day in terms of the weight of poultry or livestock that receive drinking water. It is preferable to take 2 to 0.7% lysine. Within this range, glutamine, a taste component of meat The effect of increasing acid and meat flavor can be sufficiently exerted.
  • the drinking water of the present invention can be easily produced by mixing a basic amino acid in water. By supplying this water to poultry or livestock, free dull which is a taste component in meat is obtained. The amount of tamic acid and meat flavor can be increased.
  • the poultry or livestock of the present invention is fed with a feed containing 120 to 200% lysine with respect to the lysine nutrient requirement.
  • the poultry or livestock of the present invention is fed with a feed containing 120 to 200% lysine with respect to lysine nutrient requirement and 100 to 130% arginine with respect to arginine nutrient requirement. It is.
  • the poultry or livestock of the present invention is bred with a feed containing lysine and Z or algin that exceed the standard nutritional requirements, and is rich in free glutamic acid.
  • the meat of the present invention is obtained from poultry or livestock fed with feed containing lysine and Z or arginine in excess of the above-mentioned standard nutrient requirements, and is rich in free glutamic acid. contains.
  • Feed 1 (Example 1): Corn 46.0 wt 0/0, soybean meal 19.1 wt 0/0, bran 6.0 by weight%, fish meal 2.0% by weight, corn gluten meal 5. 0 wt%, soybean oil 6.0 wt%, corn starch 2.7 wt%, calcium carbonate 1.0% by weight, dicalcium phosphate 1.3 by weight 0/0, saline 0.3 wt 0/0, vitamin 'mineral mix Chiya one 0.5 wt 0/0, the amino acid mixture 8.186 wt% (L-lysine hydrochloride 1.142 wt%, L-arginine 0.067 wt%, L Mechionin 0.095 wt 0/0, L Bok Leo - down 0.082 wt 0/0, L Aranin 0.373 wt%, L-Asuno Ragin acid 0.339 wt 0/0, L Asuno Ragin 0.438
  • Feed 2 (Comparative Example 1): Corn 43.6% by weight, soybean meal 22.5% by weight, bran 6.0% by weight, fish meal 2.0% by weight, corn gluten meal 6.6% by weight, soybean oil 6.0 wt%, corn starch 3.4 wt%, calcium carbonate 1.0% by weight, dicalcium phosphate 1.2 by weight 0/0, saline 0.3 wt 0/0, vitamin 'mineral mix Chiya one 0.5 wt 0/0, the amino acid mixture 6.048 wt% (L-lysine hydrochloride 0.032 wt%, L Mechionin 0.016 wt%, L Aranin 0.329 wt 0/0, L Asuno Ragin acid 0 .
  • the amount of free glutamate per lg of muscle was determined by the conventional method ((1) Effect of Restricted reeding before Marketing on Taste Active Components of Broiler Cnickens, 3 ⁇ 4hin obu FUJIMURA, Fumiaki SAKAI, Motoni KADOWAKl, Animal Science Journal , 72 (3), P223-229, 2001; (2) Analysis of chicken 'chemical eggs' using chemical and physical and sensory techniques Research on the use of analysis results for quality improvement, Shinobu Fujimura, Katsumi Nishito, Naoyuki Mori, Hiromi Suzuki, Akemi Yamauchi, Naoto Harada, Naoko Hashiguchi, Shiro Imai, Yumiko Ishibashi, Makiko Kiyokawa
  • FIG. 1 shows the lysine content in the feed (1,000 000% by weight (Comparative Example 1) and 2. 000% by weight (Example 1)), and the vertical axis shows the free glutamate concentration in the muscle. ing.
  • the glutamic acid concentration in the muscle increased compared to the lysine content of 1.000% by weight (Comparative Example 1) in the conventional feed. I was able to confirm that.
  • Feed 3 (Example 2): Corn 20.0 wt%, soybean meal 16.6 wt%, wheat bran 1.0 by weight 0/0, fish meal 3.0 wt 0/0, Grain Sorghum 48.0 weight 0/0, soybean oil 4.7 wt 0/0, corn starch 1.6 wt%, calcium carbonate 0.9% by weight, dicalcium phosphate 1.2 wt 0/0, saline 0.3 wt 0/0 , vitamin 'mineral mix Chiya one 0.5 wt 0/0, the amino acid mixture 1.29 5 wt% (L-lysine hydrochloride 0.722 wt%, L-arginine 0.203 wt%, L Mechi Onin 0.121 wt 0 / 0, L Toreonin 0.174 wt 0/0, L-heparin 0.051 wt 0/0, L-isoleucine 0.024 wt 0/0), agar
  • Feed 4 (Comparative Example 2): Corn 20.0 wt%, soybean meal 18.6 wt%, wheat bran 1.0 by weight 0/0, fish meal 3.0 wt 0/0, Grain Sorghum 48.0 weight 0/0, soybean oil 4.8 wt 0/0, corn starch 1.0 wt%, calcium carbonate 0.9% by weight, dicalcium phosphate 1.2 wt 0/0, saline 0.3 wt 0/0 , vitamin 'mineral mix Chiya one 0.5 wt 0/0, the amino acid mixture 0.55 2 wt 0/0 (L-lysine hydrochloride 0.163 wt 0/0, L-arginine 0.138 wt 0/0, L-Mech Old Nin 0.108 wt 0/0, L Bokuresai Nin 0.138 wt 0/0, L over ryanodine 0.005 wt 0/0), Agar 0.163 wt
  • FIG. 2 shows the lysine content in the feed (1,000% by weight (Comparative Example 2) and 1.500% by weight (Example 2)), and the vertical axis shows the free glutamate concentration in the muscle. ing.
  • the glutamic acid concentration in the muscle increased as compared to the conventional lysine content of 1.000% by weight (Comparative Example 2). I was able to confirm that.
  • Example 3 A sensory test was performed on the taste of the meat obtained in Example 2 and Comparative Example 2 above. Prepared meat was fed feed 3 (lysine content 1.500% by weight, arginine content 1.100% by weight) -meat chicken meat (Example 3) and feed 4 (lysine content 1.000% by weight) And chicken meat (Comparative Example 3) fed arginine content 1.100% by weight).
  • GDH glutamate dehydrogenase
  • GS glutamine synthetase
  • FIG. 5 the mechanism of glutamate metabolism in the animal body (muscle) of the method according to the present invention is shown in FIG.
  • glutamate dehydrogenase Glutaminase, GDH
  • glutaminase glutaminase, GA
  • glutamine synthetase GS
  • FIG. 6 shows the GDH activity in the living body (muscle)
  • FIG. 7 shows the GS activity in the living body (muscle).
  • GDH activity showed high GDH activity when the lysine content in the feed was more than 1.000% by weight.
  • GS activity showed almost the same activity even when the lysine content was 1.000 wt% or 1.500 wt%. From these results, it was found that increasing the lysine content in the feed increased the GDH activity and increased the ability to synthesize glutamate in the muscle. In addition, increasing the lysine content in the feed did not change the GS activity, but did not change the intramuscular glutamate resolution.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Birds (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

Provided is a method for increasing the amount of tastant and/or flavor in a meat of a poultry or domestic animal by controlling the ingredients in a feedstuff or drinking water for the poultry or domestic animal. Also provided are a feedstuff and a drinking water which are useful for the production of a meat of a poultry or domestic animal containing a large amount of a tastant and having good flavor and taste and which can be prepared readily. A feedstuff having a high content of a basic amino acid (e.g., lysine and/or arginine) or a drinking water containing a basic amino acid may be fed to a poultry or domestic animal. The feedstuff is characterized in that it can increase the amount of a tastant and/or flavor in the poultry or domestic animal and that it contains 120 to 200% of lysine relative to the nutritional requirement of lysine and 100 to 130% of arginine relative to the nutritional requirement of arginine. The drinking water for a poultry or domestic animal is useful for increasing the amount of a tastant and/or flavor of a meet of the poultry or domestic animal and can be prepared by mixing a basic amino acid with water.

Description

明 細 書  Specification
食肉の呈味成分量及び Z又は風味の増加方法とそのための飼料及び飲 水  Method of increasing the amount of flavor components and Z or flavor of meat, and feed and drinking water therefor
技術分野  Technical field
[oooi] 本発明は、食肉の呈味成分量及び Z又は風味の増加方法とそのための飼料及び 飲水に関し、さらに詳細には家禽又は家畜の食肉中の遊離グルタミン酸量及び Z又 は風味の増加方法とそのための飼料及び飲水に関する。  [oooi] The present invention relates to a method for increasing the amount of flavor components and Z or flavor of meat, and feed and drinking water therefor, and more particularly, a method for increasing the amount of free glutamic acid and Z or flavor in poultry or livestock meat. And feed and drinking water for it.
背景技術  Background art
[0002] 従来の家禽又は家畜飼料は、体重増加や飼料効率など食肉生産性を主要な指標 として、低コストで十分な成績を得ることを目的として設計されている。その基準として 栄養要求量があり、通常、各栄養素の量は、栄養要求量を十分量以上に充足させて 設計される。必須アミノ酸であるリジンの栄養要求量は、アメリカの NRC (National Re search Council, Nutrient Requirement of Poultry)の飼養標準(1994年版)において 3〜6週齢では飼料中に 1. 00%、 6〜8週齢では 0. 85%と設定され、世界的にこの 量がリジン栄養要求量の基準となっている。また、 日本飼養標準(2004年版)では 3 週齢以後はリジン要求量が 0. 97%と設定されている。  [0002] Conventional poultry or livestock feed is designed for the purpose of obtaining sufficient results at low cost, with meat productivity such as weight gain and feed efficiency as the main indicators. Nutritional requirements are the standard, and the amount of each nutrient is usually designed by satisfying the nutrient requirements more than sufficient. The nutritional requirement of lysine, an essential amino acid, is 1.00% in the diet at the age of 3-6 weeks according to the American National Research Council, Nutrient Requirement of Poultry (1994) breeding standard (1994 version), 6-8 It is set at 0.85% at the age of weeks, and this amount is the standard for lysine nutrition requirements worldwide. The Japanese breeding standard (2004 version) sets the required amount of lysine as 0.97% after 3 weeks of age.
[0003] 一方、アルギニンの要求量は、アメリカの NRCの飼養標準(1994年版)において 3 〜6週齢では 1. 10%、 6〜8週齢では 1. 00%と設定され、世界的にこの量がアルギ ニン要求量の基準となっている。また、日本飼養標準(2004年版)では 3週齢以後は アルギニン要求量が 1. 17%と設定されている。なお現在、 日本やアメリカ等で一般 的に使われて 、る配合飼料は、エネルギー源であるトウモロコシと植物タンパク質源 である大豆粕とを主原料とし、これを用いた配合飼料中のリジンやアルギニンの含有 量は NRC要求量の 1. 0倍程度である。  [0003] On the other hand, the required amount of arginine is set to 1.10% for 3-6 weeks old and 1.00% for 6-8 weeks old in the American NRC breeding standard (1994 version). This amount is the basis for the arginine requirement. In addition, the Japanese breeding standard (2004 version) sets the arginine requirement to 1.17% after 3 weeks of age. Currently, the mixed feed generally used in Japan, the United States, etc. is mainly composed of corn, which is an energy source, and soybean meal, which is a plant protein source, and lysine and arginine in the mixed feed using this. The content of is about 1.0 times the NRC requirement.
[0004] 上述のように、生産性の高さ(体重増加速度や飼料効率等)を主たる指標として 、 る現在の飼料では、飼料のリジンやアルギニンなどのアミノ酸割合は生産効率を優先 して設定され、栄養要求量を十分に満たせばよいとする設定の仕方であり、またコス トの面力も標準の栄養要求量以上のアミノ酸は添加されていな力つた。 [0005] ところで、食肉の高品質ィ匕を図る上で、呈味成分のひとつである肉中遊離ダルタミ ン酸濃度の調節は非常に有効であるが、これまで屠殺後の肉を熟成させて食肉の主 要呈味成分である遊離グルタミン酸量やイノシン酸量を増加ないし変動させることは 知られているものの、飼料又は飲水によって食肉の遊離グルタミン酸を増加させるこ とについては知られていな力つた。そのため飼料又は飲水の設計時において、種々 のアミノ酸量を調節すると 、う配慮はなされて 、なかった。 [0004] As described above, in the current feed with high productivity (weight gain rate, feed efficiency, etc.) as the main indicator, the ratio of amino acids such as lysine and arginine in the feed is set with priority on the production efficiency. Therefore, it was set to satisfy the nutritional requirements sufficiently, and the cost was also increased with no added amino acids exceeding the standard nutritional requirements. [0005] By the way, in order to improve the quality of meat, it is very effective to adjust the free dartamic acid concentration in the meat, which is one of the taste ingredients, but the meat after slaughtering has been aged so far. Although it is known to increase or fluctuate the amount of free glutamic acid and inosinic acid, which are the main flavor components of meat, it has not been known to increase the amount of free glutamic acid in meat by feeding or drinking water. . Therefore, no consideration has been given to adjusting the amount of various amino acids in the design of feed or drinking water.
[0006] このような状況下で本発明者らは、家禽に対するタンパク質給与量と遊離グルタミン 酸量との間に比例関係があることに着目し、家禽又は家畜に低ロイシン含量の飼料 を給与することを特徴とする食肉中の遊離グルタミン酸量の増加方法及びそのため の飼料を提案した (特許文献 1)。  [0006] Under such circumstances, the present inventors pay attention to the fact that there is a proportional relationship between the amount of protein fed to poultry and the amount of free glutamic acid, and feed poultry or livestock with a low leucine content feed. A method for increasing the amount of free glutamic acid in meat and a feed for the same have been proposed (Patent Document 1).
特許文献 1:国際公開第 WO2005Z018315号パンフレット  Patent Document 1: International Publication No. WO2005Z018315 Pamphlet
発明の開示  Disclosure of the invention
発明が解決しょうとする課題  Problems to be solved by the invention
[0007] しかしながら、通常の飼料にはロイシンは NRC要求量の 1. 3〜1. 5倍程度含まれ ており、実際、飼料中のロイシンのみの含量を低減させることは容易ではなぐまた低 ロイシン含量の原料が非常に限定されており少なぐ且つ製造工程が煩雑でありコス トが力かるといった問題があった。飼料添加用アミノ酸の中で、リジンは安価であるが 、ノ リンは高価格である。また、イソロイシンは日本では、飼料添加物として認可され ていない。 [0007] However, normal feed contains about 1.3 to 1.5 times the amount of NRC required for NRC. In fact, it is not easy to reduce the content of leucine alone in the feed. The content of raw materials is very limited, and there is a problem that the manufacturing process is complicated and the cost is high. Among the amino acids for feed addition, lysine is inexpensive, but it is expensive. Isoleucine is not approved as a feed additive in Japan.
[0008] そこで、本発明は、家禽又は家畜用の飼料又は飲水の成分調節により食肉の呈味 成分量及び Z又は風味の増加方法を提供することを目的とする。さらに、食肉の呈 味成分が多く風味や食味に優れた食肉を生産し、且つ容易に製造可能な飼料及び 飲水を提供することを目的とする。  [0008] Therefore, an object of the present invention is to provide a method for increasing the amount of flavor components and the Z or flavor of meat by adjusting the components of feed or drinking water for poultry or livestock. It is another object of the present invention to provide feed and drinking water that can produce meat that has many flavor components and is excellent in flavor and taste and that can be easily manufactured.
課題を解決するための手段  Means for solving the problem
[0009] 上記課題に鑑みて鋭意検討した結果、飼料自体に本来含まれるリジンを飼料に添 カロすることによって、肉の主たる呈味成分である遊離グルタミン酸が増加することを見 出し、本発明に想到した。 [0009] As a result of intensive studies in view of the above problems, it was found that free glutamic acid, which is a main taste component of meat, increases by adding lysine originally contained in the feed itself to the feed. I thought.
[ooio] 本発明の食肉の呈味成分量及び Z又は風味の増加方法は、塩基性アミノ酸含量 の高い飼料を家禽又は家畜に給与することを特徴とする。 [ooio] The method of increasing the taste component amount and Z or flavor of the meat of the present invention comprises the basic amino acid content. It is characterized by feeding high-powder feed to poultry or livestock.
[ooii] また、前記塩基性アミノ酸力 リジン及び Z又はアルギニンであることを特徴とする。  [ooii] Further, the basic amino acid strength is lysine and Z or arginine.
[0012] また、前記リジンの含量力 リジン栄養要求量に対して 120〜200%であることを特 徴とする。  [0012] In addition, the lysine content is 120 to 200% with respect to the lysine nutrient requirement.
[0013] また、前記アルギニンの含量力 アルギニン栄養要求量に対して 100〜130%であ ることを特徴とする。  [0013] The arginine content is 100 to 130% with respect to the arginine nutrient requirement.
[0014] さらに、塩基性アミノ酸を含む水を家禽又は家畜に給与することを特徴とする。  [0014] Further, water containing basic amino acids is supplied to poultry or livestock.
[0015] 本発明の飼料は、食肉の呈味成分量及び Z又は風味を増加させるための家禽又 は家畜用飼料であって、リジン栄養要求量に対して 120〜200%のリジンを含有する ことを特徴とする。 [0015] The feed of the present invention is a poultry or livestock feed for increasing the amount of taste components and Z or flavor of meat, and contains 120 to 200% of lysine with respect to lysine nutrient requirements. It is characterized by that.
[0016] また、アルギニン栄養要求量に対して 100〜130%のアルギニンをさらに含有する ことを特徴とする。  [0016] Further, it is characterized by further containing 100 to 130% of arginine with respect to the arginine nutrient requirement.
[0017] 本発明の飲水は、食肉の呈味成分量及び Z又は風味を増加させるための家禽又 は家畜用飲水であって、塩基性アミノ酸を水に混和したことを特徴とする。  [0017] The drinking water of the present invention is a poultry or livestock drinking water for increasing the amount of flavor components and Z or flavor of meat, characterized in that a basic amino acid is mixed in water.
[0018] 本発明の家禽又は家畜は、リジン栄養要求量に対して 120〜200%のリジンを含 有する飼料を給与されて飼育されたことを特徴とする。 [0018] The poultry or livestock of the present invention is characterized by being fed with a feed containing 120 to 200% of lysine with respect to lysine nutrient requirement.
[0019] また、リジン栄養要求量に対して 120〜200%のリジンと、アルギニン栄養要求量 に対して 100〜130%のアルギニンを含有する飼料を給与されて飼育されたことを特 徴とする。 [0019] Further, the present invention is characterized by being fed with a feed containing 120 to 200% lysine with respect to lysine nutrient requirement and 100 to 130% arginine with respect to arginine nutrient requirement. .
[0020] 本発明の食肉は、上記の家禽又は家畜から得られたことを特徴とする。  [0020] The meat of the present invention is obtained from the above-mentioned poultry or livestock.
発明の効果  The invention's effect
[0021] 本発明の食肉の呈味成分量及び Z又は風味の増加方法によれば、食肉の遊離グ ルタミン酸量を増加させることができる。この遊離グルタミン酸量の増加は、食味の向 上として判別できるものであり、食肉の付加価値を向上させることができる。  [0021] According to the method for increasing the taste component amount and Z or flavor of meat according to the present invention, the amount of free glutamic acid in the meat can be increased. This increase in the amount of free glutamic acid can be identified as an improvement in the taste, and can improve the added value of the meat.
[0022] 本発明の飼料は、標準の栄養要求量以上のリジン及び Z又はアルギニンを添加す ることにより容易に製造することができ、この飼料を家禽又は家畜に給与することによ り、食肉の遊離グルタミン酸量を増加させることができる。  [0022] The feed of the present invention can be easily produced by adding lysine and Z or arginine in excess of the standard nutritional requirements. By supplying this feed to poultry or livestock, meat is consumed. The amount of free glutamic acid can be increased.
[0023] 本発明の飲水は、塩基性アミノ酸を水に混和することにより容易に製造することがで き、この水を家禽又は家畜に給与することにより、食肉の遊離グルタミン酸量を増加さ せることができる。 [0023] The drinking water of the present invention can be easily produced by mixing a basic amino acid in water. By supplying this water to poultry or livestock, the amount of free glutamic acid in the meat can be increased.
[0024] 本発明の家禽又は家畜は、標準の栄養要求量以上のリジン及び Z又はアルギ- ンを含有する飼料を給与されて飼育されたものであり、遊離グルタミン酸を豊富に含 有する。  [0024] The poultry or livestock of the present invention is bred with a feed containing lysine and Z or algin exceeding the standard nutritional requirement, and is rich in free glutamic acid.
[0025] 本発明の食肉は、上記の家禽又は家畜力 得られたものであり、遊離グルタミン酸 を豊富に含有する。  [0025] The meat of the present invention is obtained from the above-mentioned poultry or livestock power and contains abundant free glutamic acid.
図面の簡単な説明  Brief Description of Drawings
[0026] [図 1]本発明の実施例 1における飼料中のリジン含量と食肉中の遊離グルタミン酸濃 度との関係を示すグラフである。  FIG. 1 is a graph showing the relationship between lysine content in feed and free glutamate concentration in meat in Example 1 of the present invention.
[図 2]本発明の実施例 2における飼料中のリジン含量と食肉中の遊離グルタミン酸濃 度との関係を示すグラフである。  FIG. 2 is a graph showing the relationship between lysine content in feed and free glutamate concentration in meat in Example 2 of the present invention.
[図 3]本発明の実施例 3における食肉の二点比較法による官能評価結果を示すダラ フである。  FIG. 3 is a drape showing the result of sensory evaluation by a two-point comparison method for meat in Example 3 of the present invention.
[図 4]本発明の実施例 3における食肉のシヱッフエの一対比較法による官能評価結果 を示すグラフである。  FIG. 4 is a graph showing sensory evaluation results by a paired comparison method of meat Schiffe in Example 3 of the present invention.
[図 5]本発明に関する生体内におけるグルタミン酸代謝機構を示す説明図である。  FIG. 5 is an explanatory diagram showing the mechanism of glutamate metabolism in vivo according to the present invention.
[図 6]本発明の実施例 4における飼料中のリジン含量とグルタミン酸デヒドロゲナーゼ 活性との関係を示すグラフである。  FIG. 6 is a graph showing the relationship between the lysine content in the feed and the glutamate dehydrogenase activity in Example 4 of the present invention.
[図 7]本発明の実施例 4における飼料中のリジン含量とグルタミンシンテターゼ活性と の関係を示すグラフである。  FIG. 7 is a graph showing the relationship between lysine content in feed and glutamine synthetase activity in Example 4 of the present invention.
発明を実施するための最良の形態  BEST MODE FOR CARRYING OUT THE INVENTION
[0027] 以下、本発明の食肉の呈味成分量及び Z又は風味の増加方法とそのための飼料 及び飲水について、詳細に説明する。 [0027] Hereinafter, the method of increasing the taste component amount and Z or flavor of meat of the present invention, and the feed and drinking water therefor will be described in detail.
[0028] 本発明の食肉の呈味成分量及び Z又は風味の増加方法は、塩基性アミノ酸含量 の高い飼料を家禽又は家畜に給与するものである。ここで、本発明が適用可能な家 禽としては、鶏,鴨,七面鳥,鳩,鶉など、また、家畜としては牛,豚,羊,山羊,馬な どが挙げられ、特定の家禽、家畜に限らず、幅広く適用可能である。 [0029] また、本発明に用いられる飼料に高 ヽ含量で含まれて!/ヽることが好まし ヽ塩基性ァ ミノ酸は、リジン、アルギニンである。リジン、アルギニンは経口で摂取され、肝臓で代 謝される。これら塩基性アミノ酸の少なくとも一方を従来の飼料と比較して高い含量で 飼料中に含有させ、この飼料を家禽又は家畜に給与すれば、家禽又は家畜から得ら れる食肉中の呈味成分であるグルタミン酸及び食肉風味が増加する。具体的には、 飼料にリジン、アルギニンを添加して家禽又は家畜に給与すればよい。添加用のリジ ン、アルギニンは、一般に飼料への添加用として低コストで巿販されているものを使 用することができる。 [0028] The method for increasing the taste component amount and Z or flavor of meat according to the present invention is to feed a poultry or livestock with a feed having a high basic amino acid content. Here, examples of poultry to which the present invention can be applied include chickens, duck, turkeys, pigeons, and sharks, and examples of livestock include cattle, pigs, sheep, goats, and horses. The present invention is not limited to this and can be widely applied. [0029] In addition, it is preferable that the feed used in the present invention is contained in a high cocoon content! / The basic amino acids are lysine and arginine. Lysine and arginine are taken orally and are rewarded by the liver. If at least one of these basic amino acids is contained in the feed in a high content compared to conventional feed, and if this feed is fed to poultry or livestock, it is a flavor component in meat obtained from poultry or livestock Glutamic acid and meat flavor increase. Specifically, lysine and arginine may be added to the feed and fed to poultry or livestock. As lysine and arginine for addition, those generally sold at low cost for addition to feed can be used.
[0030] 飼料中のリジンの含量は、標準のリジン栄養要求量の 120〜200%とし、さらに 14 0〜170%とするのが好ましい。なお、リジンの含量が 120%より少ない場合、食肉呈 味成分量の増加が見られず好ましくない。また、リジンの含量が 200%より多い場合 、食肉呈味成分量の増加が見られるものの、リジンの添加に要するコストが増加する ので好ましくない。なお、標準のリジン栄養要求量は、アメリカの NRC (National Rese arch Council, Nutrient Requirement of Poultry)の飼養標準(1994年版)に基づく。  [0030] The lysine content in the feed is preferably 120 to 200%, more preferably 140 to 170% of the standard lysine nutrient requirement. In addition, when the content of lysine is less than 120%, an increase in the amount of meat taste components is not observed, which is not preferable. In addition, when the content of lysine is more than 200%, although the amount of meat-flavoring components is increased, the cost required for the addition of lysine is not preferable. The standard lysine nutrient requirements are based on the breeding standards (1994 version) of the American National Research Council (NRC), Nutrient Requirement of Poultry.
[0031] 飼料中のアルギニンの含量は、標準のアルギニン栄養要求量の 100〜130%とす るのが好ましい。アルギニンの含量が 100%より少ない場合、食肉呈味成分量の増 加が見られず好ましくない。また、アルギニンの含量が 130%より多い場合、食肉呈 味成分量の増加が見られるものの、アルギニンの添カ卩に要するコストが増加するので 好ましくない。なお、標準のアルギニン栄養要求量は、 NRCの飼養標準(1994年版 )に基づく。  [0031] The content of arginine in the feed is preferably 100 to 130% of the standard arginine nutrient requirement. When the content of arginine is less than 100%, an increase in the amount of meat taste components is not observed, which is not preferable. In addition, when the content of arginine is more than 130%, although the amount of meat-tasting components is increased, the cost required for adding arginine is not preferable. The standard arginine nutrient requirements are based on the NRC breeding standard (1994 edition).
[0032] なお、本発明の飼料の給与方法としては、本発明の飼料を少なくとも出荷前 2日以 降から出荷直前まで給与することが好ましぐさらには出荷前 10日以降力 出荷直 前まで給与することが好ましい。このように出荷前の数日間から出荷直前まで給与す ることによって、リジン及び Z又はアルギニン含量の高い飼料に力かるコストを抑える ことができ、また出荷直前に食肉の呈味成分を増加することができる。  [0032] As a feed method of the feed of the present invention, it is preferable to feed the feed of the present invention from at least 2 days before shipment to immediately before shipment, and even after 10 days before shipment, until immediately before shipment. It is preferable to pay. In this way, by feeding from several days before shipment to just before shipment, the cost of working on feeds with high lysine and Z or arginine content can be reduced, and the flavor component of meat can be increased just before shipment. Can do.
[0033] また、本発明の食肉の呈味成分量及び Z又は風味の増加方法は、塩基性アミノ酸 を含む水を家禽又は家畜に給与するものである。この場合、家禽又は家畜の飲水中 に塩基性アミノ酸としてリジンを含有させるのが好ましい。飲水中のリジンの含量は、 0 . 1〜2. 0質量%の範囲内とするのが好ましぐさらに 0. 2〜0. 7質量%の範囲内と するのが好ましい。この範囲内においては、食肉の呈味成分であるグルタミン酸及び 食肉風味の増加作用を十分に発揮することができる。塩基性アミノ酸を含む水は、家 禽又は家畜舎の給水器より家禽又は家畜に給与すればよい。 [0033] Further, the method for increasing the taste component amount and Z or flavor of meat according to the present invention supplies water containing basic amino acids to poultry or livestock. In this case, it is preferable to contain lysine as a basic amino acid in drinking water of poultry or livestock. The lysine content in drinking water is 0 It is preferable to be in the range of 1 to 2.0% by mass, and it is more preferable to be in the range of 0.2 to 0.7% by mass. Within this range, it is possible to sufficiently exert glutamic acid, which is a meat taste component, and meat flavor. Water containing basic amino acids may be supplied to poultry or livestock from a poultry or livestock water dispenser.
[0034] 本発明の食肉の呈味成分量及び Z又は風味の増加方法によれば、食肉の遊離グ ルタミン酸量を増加させることができる。この遊離グルタミン酸量の増加は、食味の向 上として判別できるものであり、食肉の付加価値を向上させることができ、食肉の高品 質ィ匕ゃ消費拡大を図ることができる。また、グルタミン酸量を増加させることによって 食肉のうま味が増強されるので、調理時の化学調味料の使用量を減少させることが できる。したがって、本発明の方法により生産した食肉は、天然の食材を重視し化学 調味料の使用量を減少させようとする最近の消費動向にも合致する。さらに、本発明 の方法は、屠殺後の肉の熟成や加工処理による呈味成分を増大させる方法と比較し 、簡便に食肉の呈味成分及び Z又は風味を増大させることができる。屠殺後の熟成 を経ても、通常飼育群よりも高いグルタミン酸量となる。  [0034] According to the method for increasing the taste component amount and Z or flavor of meat according to the present invention, the amount of free glutamic acid in the meat can be increased. This increase in the amount of free glutamic acid can be discriminated as an improvement in the taste, which can improve the added value of the meat and increase the consumption of high quality meat. Moreover, since the umami of meat is enhanced by increasing the amount of glutamic acid, the amount of chemical seasoning used during cooking can be reduced. Therefore, the meat produced by the method of the present invention is consistent with the recent consumption trend to reduce the amount of chemical seasonings used with emphasis on natural ingredients. Furthermore, the method of the present invention can easily increase the taste component and Z or flavor of meat as compared with the method of increasing the taste component by aging and processing of meat after slaughter. Even after aging after slaughter, the amount of glutamic acid is higher than that of the normal rearing group.
[0035] 本発明の飼料は、食肉の呈味成分量及び Z又は風味を増加させるための家禽又 は家畜用飼料であって、リジン栄養要求量に対して 120〜200%のリジンを含有する ものである。また、アルギニン栄養要求量に対して 100〜130%のアルギニンをさら に含有するものであってもよ 、。  [0035] The feed of the present invention is a poultry or livestock feed for increasing the amount of flavor components and Z or flavor of meat, and contains 120 to 200% of lysine with respect to lysine nutrient requirements. Is. It may also contain 100-130% arginine relative to the arginine nutrient requirements.
[0036] 本発明の飼料は、標準の栄養要求量以上のリジン及び Z又はアルギニンを添加す ることにより容易に製造することができ、この飼料を家禽又は家畜に給与することによ り、食肉中の呈味成分である遊離グルタミン酸量及び食肉風味を増加させることがで きる。  [0036] The feed of the present invention can be easily produced by adding lysine and Z or arginine in excess of the standard nutritional requirements, and by supplying this feed to poultry or livestock, It is possible to increase the amount of free glutamic acid and the flavor of meat, which are the taste components in it.
[0037] 本発明の飲水は、食肉の呈味成分量及び Z又は風味を増加させるための家禽又 は家畜用飲水であって、塩基性アミノ酸を水に混和したものである。  [0037] The drinking water of the present invention is poultry or livestock drinking water for increasing the amount of flavor components and Z or flavor of meat, and is obtained by mixing basic amino acids with water.
[0038] 塩基性アミノ酸としては、リジンを含有させるのが好ましい。飲水中のリジンの含量 は、飲水を給与する家禽又は家畜の体重換算で、 1日あたり 0. 1〜2. 0%のリジンを 摂取させる量とするのが好ましぐさらには 1日あたり 0. 2〜0. 7%のリジンを摂取さ せる量とするのが好ましい。この範囲内において、食肉の呈味成分であるグルタミン 酸及び食肉風味の増加作用を十分に発揮することができる。 [0038] The basic amino acid preferably contains lysine. The content of lysine in drinking water is preferably an amount that allows intake of 0.1 to 2.0% lysine per day in terms of the weight of poultry or livestock that receive drinking water. It is preferable to take 2 to 0.7% lysine. Within this range, glutamine, a taste component of meat The effect of increasing acid and meat flavor can be sufficiently exerted.
[0039] 本発明の飲水は、塩基性アミノ酸を水に混和することにより容易に製造することがで き、この水を家禽又は家畜に給与することにより、食肉中の呈味成分である遊離ダル タミン酸量及び食肉風味を増加させることができる。  [0039] The drinking water of the present invention can be easily produced by mixing a basic amino acid in water. By supplying this water to poultry or livestock, free dull which is a taste component in meat is obtained. The amount of tamic acid and meat flavor can be increased.
[0040] 本発明の家禽又は家畜は、リジン栄養要求量に対して 120〜200%のリジンを含 有する飼料を給与されて飼育されたものである。或いは、本発明の家禽又は家畜は 、リジン栄養要求量に対して 120〜200%のリジンと、アルギニン栄養要求量に対し て 100〜 130%のアルギニンを含有する飼料を給与されて飼育されたものである。  [0040] The poultry or livestock of the present invention is fed with a feed containing 120 to 200% lysine with respect to the lysine nutrient requirement. Alternatively, the poultry or livestock of the present invention is fed with a feed containing 120 to 200% lysine with respect to lysine nutrient requirement and 100 to 130% arginine with respect to arginine nutrient requirement. It is.
[0041] 本発明の家禽又は家畜は、標準の栄養要求量以上のリジン及び Z又はアルギ- ンを含有する飼料を給与されて飼育されたものであり、遊離グルタミン酸を豊富に含 有する。  [0041] The poultry or livestock of the present invention is bred with a feed containing lysine and Z or algin that exceed the standard nutritional requirements, and is rich in free glutamic acid.
[0042] 本発明の食肉は、上記の標準の栄養要求量以上のリジン及び Z又はアルギニンを 含有する飼料を給与されて飼育された家禽又は家畜から得られたものであり、遊離 グルタミン酸を豊富に含有する。  [0042] The meat of the present invention is obtained from poultry or livestock fed with feed containing lysine and Z or arginine in excess of the above-mentioned standard nutrient requirements, and is rich in free glutamic acid. contains.
[0043] 以下の実施例及び比較例に基づいて本発明をさらに詳細に説明するが、本発明 はこれら実施例及び比較例により何ら制限されるものではない。 [0043] The present invention will be described in more detail based on the following examples and comparative examples, but the present invention is not limited to these examples and comparative examples.
実施例 1  Example 1
[0044] 各群 12羽の-ヮトリ(Cobb系雌ブロイラー)に対し、 4週齢力も屠殺直前までの 10 日間、飼料 1 (実施例 1)又は飼料 2 (比較例 1)の何れかを継続的に給与した。給与し た各飼料の組成を以下に示す。また、飼育中の測定結果は表 1に示すとおりであつ た。  [0044] For each group of 12-rooster birds (Cobb female broilers), continue either feed 1 (Example 1) or feed 2 (Comparative Example 1) for 10 days before killing at 4 weeks of age. Paid. The composition of each feed fed is shown below. The measurement results during breeding were as shown in Table 1.
[0045] 飼料 1 (実施例 1):トウモロコシ 46. 0重量0 /0、大豆粕 19. 1重量0 /0、フスマ 6. 0重 量%、魚粉 2. 0重量%、コーングルテンミール 5. 0重量%、大豆油 6. 0重量%、コ ーンスターチ 2. 7重量%、炭酸カルシウム 1. 0重量%、第二リン酸カルシウム 1. 3重 量0 /0、食塩 0. 3重量0 /0、ビタミン 'ミネラルミクスチヤ一 0. 5重量0 /0、アミノ酸混合物 8 . 186重量%(L リジン塩酸塩 1. 142重量%、 L アルギニン 0. 067重量%、 L メチォニン 0. 095重量0 /0、 L 卜レオ-ン 0. 082重量0 /0、 L ァラニン 0. 373重量 %、 L—ァスノ ラギン酸 0. 339重量0 /0、 L ァスノ ラギン 0. 438重量0 /0、 L シスチ ン 0. 035重量0 /0、 L グルタミン酸 1. 172重量0 /0、 L—グルタミン 1. 353重量0 /0、 L —グリシン 0. 216重量0 /0、 L プロリン 1. 537重量0 /0、 L—セリン 0. 619重量0 /0、 L ーチロシン 0. 717重量%)、寒天 1. 9重量%(代謝エネルギー 3. 2kcalZg、粗タン パク質 25. 35%)。なお、飼料 1中のリジン及びアルギニンの含量は、それぞれ 2. 0 00重量0 /0、 1. 100重量0 /0とした。 [0045] Feed 1 (Example 1): Corn 46.0 wt 0/0, soybean meal 19.1 wt 0/0, bran 6.0 by weight%, fish meal 2.0% by weight, corn gluten meal 5. 0 wt%, soybean oil 6.0 wt%, corn starch 2.7 wt%, calcium carbonate 1.0% by weight, dicalcium phosphate 1.3 by weight 0/0, saline 0.3 wt 0/0, vitamin 'mineral mix Chiya one 0.5 wt 0/0, the amino acid mixture 8.186 wt% (L-lysine hydrochloride 1.142 wt%, L-arginine 0.067 wt%, L Mechionin 0.095 wt 0/0, L Bok Leo - down 0.082 wt 0/0, L Aranin 0.373 wt%, L-Asuno Ragin acid 0.339 wt 0/0, L Asuno Ragin 0.438 wt 0/0, L Shisuchi Down 0.035 weight 0/0, L-glutamic acid 1.172 weight 0/0, L- glutamine 1.353 weight 0/0, L - glycine 0.216 weight 0/0, L-proline 1.537 weight 0/0 , L- serine 0.619 wt 0/0, L Chiroshin 0.717 wt%), agar 1.9 wt% (metabolizable energy 3. 2KcalZg, crude protein 25.35%). Incidentally, the content of lysine and arginine in feed 1 each 2.0 00 wt 0/0, was 1.100 wt 0/0.
[0046] 飼料 2 (比較例 1):トウモロコシ 43. 6重量%、大豆粕 22. 5重量%、フスマ 6. 0重 量%、魚粉 2. 0重量%、コーングルテンミール 6. 6重量%、大豆油 6. 0重量%、コ ーンスターチ 3. 4重量%、炭酸カルシウム 1. 0重量%、第二リン酸カルシウム 1. 2重 量0 /0、食塩 0. 3重量0 /0、ビタミン 'ミネラルミクスチヤ一 0. 5重量0 /0、アミノ酸混合物 6 . 048重量%(L リジン塩酸塩 0. 032重量%、 L メチォニン 0. 016重量%、 L ァラニン 0. 329重量0 /0、 Lーァスノ ラギン酸 0. 299重量0 /0、 Lーァスノ ラギン 0. 38 6重量0 /0、 L—シスチン 0. 031重量0 /0、 Lーグノレタミン酸 1. 034重量0 /0、 Lーグノレタミ ン 1. 194重量0 /0、 L グリシン 0. 190重量0 /0、 L—プロリン 1. 357重量0 /0、 L セリ ン 0. 546重量%、 Lーチロシン 0. 632重量0 /0)、寒天 0. 9重量0 /0 (代謝エネルギー 3 . 2kcal/g、粗タンパク質 25. 35%)。なお、飼料 2中のリジン及びアルギニンの含量 は、それぞれ 1. 000重量%、 1. 164重量%とした。 [0046] Feed 2 (Comparative Example 1): Corn 43.6% by weight, soybean meal 22.5% by weight, bran 6.0% by weight, fish meal 2.0% by weight, corn gluten meal 6.6% by weight, soybean oil 6.0 wt%, corn starch 3.4 wt%, calcium carbonate 1.0% by weight, dicalcium phosphate 1.2 by weight 0/0, saline 0.3 wt 0/0, vitamin 'mineral mix Chiya one 0.5 wt 0/0, the amino acid mixture 6.048 wt% (L-lysine hydrochloride 0.032 wt%, L Mechionin 0.016 wt%, L Aranin 0.329 wt 0/0, L Asuno Ragin acid 0 . 299 weight 0/0, L Asuno Ragin 0.38 6 wt 0/0, L-cystine 0.031 wt 0/0, L Gunoretamin acid 1.034 wt 0/0, L Gunoretami down 1.194 wt 0 / 0, L-glycine 0.190 wt 0/0, L-proline 1.357 wt 0/0, L serine 0.546 wt%, L Chiroshin 0.632 wt 0/0), agar 0.9 wt 0 / 0 (metabolism d Energy 3. 2 kcal / g, crude protein 25.35%). The contents of lysine and arginine in feed 2 were 1.000% by weight and 1.164% by weight, respectively.
[0047] [表 1]  [0047] [Table 1]
飼育成績結果  Rearing results
Figure imgf000010_0001
Figure imgf000010_0001
[0048] 屠殺後、筋肉 lg当たりに含まれる遊離グルタミン酸量を常法((1) Effect of Restrict ed reeding before Marketing on Taste Active Components of Broiler Cnickens, ¾hin obu FUJIMURA, Fumiaki SAKAI, Motoni KADOWAKl, Animal Science Journal, 72( 3), P223-229, 2001 ; (2)鶏肉'鶏卵の化学的'物理的及び官能的手法による解析並 びに解析結果の品質改善への活用に関する研究、藤村 忍、西藤克己、森 尚之、 鈴木ひろみ、山内章江、原田直人、橋口尚子、今井士郎、石橋裕美子、清川真千子[0048] After slaughter, the amount of free glutamate per lg of muscle was determined by the conventional method ((1) Effect of Restricted reeding before Marketing on Taste Active Components of Broiler Cnickens, ¾hin obu FUJIMURA, Fumiaki SAKAI, Motoni KADOWAKl, Animal Science Journal , 72 (3), P223-229, 2001; (2) Analysis of chicken 'chemical eggs' using chemical and physical and sensory techniques Research on the use of analysis results for quality improvement, Shinobu Fujimura, Katsumi Nishito, Naoyuki Mori, Hiromi Suzuki, Akemi Yamauchi, Naoto Harada, Naoko Hashiguchi, Shiro Imai, Yumiko Ishibashi, Makiko Kiyokawa
、堀口恵子『農畜産業振興事業団平成 11年度畜産物需要開発調査研究事業報告 書』、 P56-79, 2000等に記載)により測定した。 , Described in Keiko Horiguchi, “Agricultural and Livestock Industry Promotion Corporation, 1999 Livestock Product Demand Development Research Project Report”, P56-79, 2000, etc.).
[0049] 結果を図 1に示す。なお、図 1の横軸は飼料中のリジン含量(1. 000重量% (比較 例 1)及び 2. 000重量%(実施例 1) )を示し、縦軸は筋肉中の遊離グルタミン酸濃度 を示している。図 1に示されるように、本発明の方法を適用した場合には、従来の飼 料中のリジン含量 1. 000重量% (比較例 1)と比べて筋肉中のグルタミン酸濃度が増 加して 、ることが確認できた。 [0049] The results are shown in FIG. The horizontal axis in Fig. 1 shows the lysine content in the feed (1,000 000% by weight (Comparative Example 1) and 2. 000% by weight (Example 1)), and the vertical axis shows the free glutamate concentration in the muscle. ing. As shown in FIG. 1, when the method of the present invention was applied, the glutamic acid concentration in the muscle increased compared to the lysine content of 1.000% by weight (Comparative Example 1) in the conventional feed. I was able to confirm that.
実施例 2  Example 2
[0050] 各群 12羽の-ヮトリ(Cobb系雌ブロイラー)に対し、 4週齢力も屠殺直前までの 10 日間、飼料 3 (実施例 2)又は飼料 4 (比較例 2)の何れかを継続的に給与した。給与し た各飼料の組成を以下に示す。また、飼育中の測定結果は表 2に示すとおりであつ た。  [0050] For each group of 12 chicks (Cobb female broilers), continue either feed 3 (Example 2) or feed 4 (Comparative Example 2) for 10 days until just before slaughter. Paid. The composition of each feed fed is shown below. Table 2 shows the measurement results during breeding.
[0051] 飼料 3 (実施例 2):トウモロコシ 20. 0重量%、大豆粕 16. 6重量%、フスマ 1. 0重 量0 /0、魚粉 3. 0重量0 /0、グレインソルガム 48. 0重量0 /0、大豆油 4. 7重量0 /0、コーン スターチ 1. 6重量%、炭酸カルシウム 0. 9重量%、第二リン酸カルシウム 1. 2重量0 /0 、食塩 0. 3重量0 /0、ビタミン 'ミネラルミクスチヤ一 0. 5重量0 /0、アミノ酸混合物 1. 29 5重量%(L リジン塩酸塩 0. 722重量%、 L アルギニン 0. 203重量%、 Lーメチ ォニン 0. 121重量0 /0、 Lートレオニン 0. 174重量0 /0、 L—パリン 0. 051重量0 /0、 L— イソロイシン 0. 024重量0 /0)、寒天 0. 9重量0 /0 (代謝エネルギー 3. 2kcalZg、粗タン パク質 16. 9%)。なお、飼料 3中のリジン及びアルギニンの含量は、それぞれ 1. 50 0重量%、 1. 100重量%とした。 [0051] Feed 3 (Example 2): Corn 20.0 wt%, soybean meal 16.6 wt%, wheat bran 1.0 by weight 0/0, fish meal 3.0 wt 0/0, Grain Sorghum 48.0 weight 0/0, soybean oil 4.7 wt 0/0, corn starch 1.6 wt%, calcium carbonate 0.9% by weight, dicalcium phosphate 1.2 wt 0/0, saline 0.3 wt 0/0 , vitamin 'mineral mix Chiya one 0.5 wt 0/0, the amino acid mixture 1.29 5 wt% (L-lysine hydrochloride 0.722 wt%, L-arginine 0.203 wt%, L Mechi Onin 0.121 wt 0 / 0, L Toreonin 0.174 wt 0/0, L-heparin 0.051 wt 0/0, L-isoleucine 0.024 wt 0/0), agar 0.9 wt 0/0 (metabolic energy 3. 2KcalZg , Crude protein 16.9%). The contents of lysine and arginine in feed 3 were 1.500% by weight and 1.100% by weight, respectively.
[0052] 飼料 4 (比較例 2):トウモロコシ 20. 0重量%、大豆粕 18. 6重量%、フスマ 1. 0重 量0 /0、魚粉 3. 0重量0 /0、グレインソルガム 48. 0重量0 /0、大豆油 4. 8重量0 /0、コーン スターチ 1. 0重量%、炭酸カルシウム 0. 9重量%、第二リン酸カルシウム 1. 2重量0 /0 、食塩 0. 3重量0 /0、ビタミン 'ミネラルミクスチヤ一 0. 5重量0 /0、アミノ酸混合物 0. 55 2重量0 /0 (L—リジン塩酸塩 0. 163重量0 /0、 L—アルギニン 0. 138重量0 /0、Lーメチ 才ニン 0. 108重量0 /0、 L 卜レ才ニン 0. 138重量0 /0、 Lーノ ジン 0. 005重量0 /0)、寒 天 0. 1重量0 /0 (代謝エネルギー 3. 2kcalZg、粗タンパク質 16. 9%)。なお、飼料 4 中のリジン及びアルギニンの含量は、それぞれ 1. 000重量%、 1. 100重量%とした [0052] Feed 4 (Comparative Example 2): Corn 20.0 wt%, soybean meal 18.6 wt%, wheat bran 1.0 by weight 0/0, fish meal 3.0 wt 0/0, Grain Sorghum 48.0 weight 0/0, soybean oil 4.8 wt 0/0, corn starch 1.0 wt%, calcium carbonate 0.9% by weight, dicalcium phosphate 1.2 wt 0/0, saline 0.3 wt 0/0 , vitamin 'mineral mix Chiya one 0.5 wt 0/0, the amino acid mixture 0.55 2 wt 0/0 (L-lysine hydrochloride 0.163 wt 0/0, L-arginine 0.138 wt 0/0, L-Mech Old Nin 0.108 wt 0/0, L Bokuresai Nin 0.138 wt 0/0, L over ryanodine 0.005 wt 0/0), Agar 0.1 wt 0/0 (metabolizable energy 3. 2kcalZg, crude protein 16.9%). The contents of lysine and arginine in feed 4 were 1.000% by weight and 1.100% by weight, respectively.
[0053] [表 2] [0053] [Table 2]
飼育成績結果  Rearing results
Figure imgf000012_0001
Figure imgf000012_0001
[0054] 屠殺後、筋肉 lg当たりに含まれる遊離グルタミン酸量を実施例 1と同様に常法によ り測定した。  [0054] After sacrifice, the amount of free glutamic acid contained in 1 g of muscle was measured in the same manner as in Example 1.
[0055] 結果を図 2に示す。なお、図 2の横軸は飼料中のリジン含量(1. 000重量% (比較 例 2)及び 1. 500重量%(実施例 2) )を示し、縦軸は筋肉中の遊離グルタミン酸濃度 を示している。図 2に示されるように、本発明の方法を適用した場合には、従来の飼 料中のリジン含量 1. 000重量% (比較例 2)と比べて筋肉中のグルタミン酸濃度が増 加して 、ることが確認できた。  [0055] The results are shown in FIG. The horizontal axis in Fig. 2 shows the lysine content in the feed (1,000% by weight (Comparative Example 2) and 1.500% by weight (Example 2)), and the vertical axis shows the free glutamate concentration in the muscle. ing. As shown in FIG. 2, when the method of the present invention was applied, the glutamic acid concentration in the muscle increased as compared to the conventional lysine content of 1.000% by weight (Comparative Example 2). I was able to confirm that.
実施例 3  Example 3
[0056] 上記実施例 2及び比較例 2で得られた食肉の食味について官能試験を行った。調 查した肉は、飼料 3 (リジン含量 1. 500重量%、アルギニン含量 1. 100重量%)を給 与した-ヮトリの肉(実施例 3)、及び飼料 4 (リジン含量 1. 000重量%、アルギニン含 量 1. 100重量%)を給与したニヮトリの肉(比較例 3)である。  [0056] A sensory test was performed on the taste of the meat obtained in Example 2 and Comparative Example 2 above. Prepared meat was fed feed 3 (lysine content 1.500% by weight, arginine content 1.100% by weight) -meat chicken meat (Example 3) and feed 4 (lysine content 1.000% by weight) And chicken meat (Comparative Example 3) fed arginine content 1.100% by weight).
[0057] パネラーの数は 16名であり、(1)両者の肉の食味に差がある力否かを二点比較法 により調査し、また(2)肉の味の特徴をシエツフエの一対比較法((1)新版官能検査 ハンドブック、 日科技連官能検査委員会、 日科技連出版社、 1973年;(2) Effect of Re stricted Feeding before Marketing on Taste Active Components of Broiler Chickens Shinobu FUJIMURA, Fumiaki SAKAI, Motoni KADOWAKl, Animal Science Journal, 72(3), P223-229, 2001)により比較した。 [0057] The number of panelists was 16, and (1) investigated whether or not there was a difference in the taste of the meat between the two by a two-point comparison method, and (2) paired comparison of the taste of meat Act ((1) New Edition Sensory Inspection Handbook, Nikka Giren Sensory Testing Committee, Nikka Giren Publisher, 1973; (2) Effect of Restricted Feeding before Marketing on Taste Active Components of Broiler Chickens Shinobu FUJIMURA, Fumiaki SAKAI, Motoni KADOWAKl, Animal Science Journal, 72 (3), P223-229, 2001).
[0058] 二点比較法の結果を図 3に示し、シエツフエの一対比較法の結果を図 4に示す。図 3に示されるように、パネラー 16人中 16人力 実施例 3と比較例 3の肉について、味 に差があることを認めた。また、図 4に示されるように、本発明を適用して得られた食 肉(実施例 3)は、うま味、味の濃さ、総合評価に対して有意に優れていると評価され た。 [0058] The results of the two-point comparison method are shown in FIG. 3, and the results of the Sietzue paired comparison method are shown in FIG. As shown in FIG. 3, it was recognized that there was a difference in taste between the meat of Example 3 and Comparative Example 3 in 16 panelists. Further, as shown in FIG. 4, the meat obtained by applying the present invention (Example 3) was evaluated to be significantly superior to umami, taste intensity, and comprehensive evaluation.
実施例 4  Example 4
[0059] 上記実施例 3及び比較例 3のそれぞれの肉の筋肉内のグルタミン酸デヒドロゲナー ゼ (GDH)活性及びグルタミンシンテターゼ (GS)活性を測定した。  [0059] The glutamate dehydrogenase (GDH) activity and glutamine synthetase (GS) activity in the muscle muscle of each of Example 3 and Comparative Example 3 were measured.
[0060] まず、本発明による方法の動物生体 (筋肉)内におけるグルタミン酸代謝機構を図 5 に示す。図 5に示されるように、生体 (筋肉)内のグルタミン酸の合成、分解には α— ケトグルタル酸を基質とするグルタミン酸デヒドロゲナーゼ(Glutamate dehydrogenase , GDH)、グルタミンを基質とするグルタミナーゼ(Glutaminase, GA)、グルタミン酸を 基質とするグルタミンシンテターゼ(Glutamine Synthetase, GS)が影響する。  [0060] First, the mechanism of glutamate metabolism in the animal body (muscle) of the method according to the present invention is shown in FIG. As shown in Fig. 5, glutamate dehydrogenase (Glutaminase, GDH) using α-ketoglutarate as a substrate, glutaminase (Glutaminase, GA) using glutamine as a substrate, Glutamine synthetase (GS) using glutamic acid as a substrate is affected.
[0061] 生体 (筋肉)内の GDH活性を図 6に示し、生体 (筋肉)内の GS活性を図 7に示す。  FIG. 6 shows the GDH activity in the living body (muscle), and FIG. 7 shows the GS activity in the living body (muscle).
図 6に示すように、 GDH活性は飼料中のリジン含量が 1. 000重量%より多い場合、 高い GDH活性を示した。一方、図 7に示すように、 GS活性はリジン含量が 1. 000重 量%又は 1. 500重量%でもほぼ同程度の活性を示した。これらの結果から、飼料中 のリジン含量を増加させることにより GDH活性が増加し、筋肉中のグルタミン酸合成 能が高まることが判明した。また、飼料中のリジン含量を増加させても、 GS活性は変 わらず筋肉内グルタミン酸分解能に変化は見られな力つた。  As shown in FIG. 6, GDH activity showed high GDH activity when the lysine content in the feed was more than 1.000% by weight. On the other hand, as shown in FIG. 7, GS activity showed almost the same activity even when the lysine content was 1.000 wt% or 1.500 wt%. From these results, it was found that increasing the lysine content in the feed increased the GDH activity and increased the ability to synthesize glutamate in the muscle. In addition, increasing the lysine content in the feed did not change the GS activity, but did not change the intramuscular glutamate resolution.

Claims

請求の範囲  The scope of the claims
[I] 塩基性アミノ酸含量の高い飼料を家禽又は家畜に給与することを特徴とする食肉の 呈味成分量及び Z又は風味の増加方法。  [I] A method for increasing the amount of flavor components and Z or flavor of meat, characterized by feeding a poultry or livestock feed with a high basic amino acid content.
[2] 前記塩基性アミノ酸力 リジン及び Z又はアルギニンであることを特徴とする請求の 範囲 1記載の食肉の呈味成分量及び Z又は風味の増加方法。  [2] The method according to claim 1, wherein the basic amino acid strength is lysine and Z or arginine.
[3] 前記リジンの含量力 リジン栄養要求量に対して 120〜200%であることを特徴とす る請求の範囲 2記載の食肉の呈味成分量及び Z又は風味の増加方法。  [3] The method according to claim 2, wherein the lysine content is 120 to 200% of the lysine nutrient requirement.
[4] 前記アルギニンの含量力 アルギニン栄養要求量に対して 100〜130%であることを 特徴とする請求の範囲 2記載の食肉の呈味成分量及び Z又は風味の増加方法。 [4] The content of the arginine, which is 100 to 130% with respect to the arginine nutrient requirement, The method of increasing the amount of flavor components and Z or flavor of the meat according to claim 2.
[5] 塩基性アミノ酸を含む水を家禽又は家畜に給与することを特徴とする食肉の呈味成 分量及び Z又は風味の増加方法。 [5] A method for increasing the flavor content and the Z or flavor of meat, characterized in that water containing basic amino acids is fed to poultry or livestock.
[6] 食肉の呈味成分量及び Z又は風味を増加させるための家禽又は家畜用飼料であつ て、リジン栄養要求量に対して 120〜200%のリジンを含有することを特徴とする飼 料。 [6] Poultry or livestock feed for increasing the amount of flavor components and Z or flavor of meat, characterized by containing 120 to 200% lysine with respect to lysine nutrient requirements .
[7] アルギニン栄養要求量に対して 100〜130%のアルギニンをさらに含有することを特 徴とする請求の範囲 6記載の飼料。  [7] The feed according to claim 6, further comprising 100 to 130% of arginine relative to the arginine nutrient requirement.
[8] 食肉の呈味成分量及び Z又は風味を増加させるための家禽又は家畜用飲水であつ て、塩基性アミノ酸を水に混和したことを特徴とする飲水。 [8] Drinking water for poultry or livestock to increase the amount of flavor components and Z or flavor of meat, characterized by mixing basic amino acids with water.
[9] リジン栄養要求量に対して 120〜200%のリジンを含有する飼料を給与されて飼育さ れたことを特徴とする家禽又は家畜。 [9] Poultry or livestock fed with feed containing 120 to 200% lysine with respect to lysine nutrient requirements.
[10] リジン栄養要求量に対して 120〜200%のリジンと、アルギニン栄養要求量に対して[10] 120-200% lysine relative to lysine nutrient requirements and arginine nutrient requirements
100〜 130%のアルギニンを含有する飼料を給与されて飼育されたことを特徴とする 家禽又は家畜。 Poultry or livestock raised with feed containing 100 to 130% arginine.
[II] 請求の範囲 9又は 10に記載の家禽又は家畜力も得られたことを特徴とする食肉。  [II] Meat characterized in that the poultry or livestock power described in claim 9 or 10 is also obtained.
PCT/JP2006/301828 2005-03-18 2006-02-03 Method for increasing amount of tastant and/or flavor in meat and feedstuff and drinking water for the same WO2006100838A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2007509158A JP4945762B2 (en) 2005-03-18 2006-02-03 Method for increasing the amount of free glutamic acid in meat and its feed

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2005-079375 2005-03-18
JP2005079375 2005-03-18

Publications (1)

Publication Number Publication Date
WO2006100838A1 true WO2006100838A1 (en) 2006-09-28

Family

ID=37023524

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2006/301828 WO2006100838A1 (en) 2005-03-18 2006-02-03 Method for increasing amount of tastant and/or flavor in meat and feedstuff and drinking water for the same

Country Status (3)

Country Link
JP (1) JP4945762B2 (en)
TW (1) TW200700017A (en)
WO (1) WO2006100838A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009225752A (en) * 2008-03-25 2009-10-08 Niigata Univ Method for increasing free glutamic acid in meat
JP2011055792A (en) * 2009-09-11 2011-03-24 Aska Pharmaceutical Co Ltd Poultry feed for improving meat texture, method for producing meat having improved texture, and method for breeding poultry, and agent for softening poultry meat

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111903847A (en) * 2020-07-17 2020-11-10 山东农微生物科技有限公司 Serine feed additive for promoting production performance of meat poultry and application thereof
WO2023008286A1 (en) * 2021-07-29 2023-02-02 日本水産株式会社 Method for improving poultry meat quality, poultry meat processed product and poultry meat quality-improving feed, and broiler and chicken meat

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000189066A (en) * 1998-12-28 2000-07-11 Yoshio Inoue Drinking water containing mineral (zinc) for domestic animal and amino acid

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59216551A (en) * 1983-05-25 1984-12-06 Itochu Shiryo Kk Feed for chicken
JPH02231041A (en) * 1989-03-06 1990-09-13 Itochu Shiryo Kk Low protein feed for growing poultry
JP2935704B1 (en) * 1998-09-11 1999-08-16 全国農業協同組合連合会 Pig raising method and feed used for it
JP2001086940A (en) * 1999-09-22 2001-04-03 Ajinomoto Co Inc Feed improver for lactating cow
JP2001299233A (en) * 2000-04-27 2001-10-30 Natl Fedelation Of Agricult Coop Assoc Method of decreasing urine of pig and feed therefor

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000189066A (en) * 1998-12-28 2000-07-11 Yoshio Inoue Drinking water containing mineral (zinc) for domestic animal and amino acid

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WALDROUP P.W. ET AL.: "Dietary Arginine and Lysine in Large White Toms. 1. Increasing Arginine: Lysine Ratios Does Not Improve Performance When Lysine Levels Are Adequate", POULTRY SCIENCE, vol. 77, no. 9, 1998, pages 1364 - 1370, XP002999180 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009225752A (en) * 2008-03-25 2009-10-08 Niigata Univ Method for increasing free glutamic acid in meat
JP2011055792A (en) * 2009-09-11 2011-03-24 Aska Pharmaceutical Co Ltd Poultry feed for improving meat texture, method for producing meat having improved texture, and method for breeding poultry, and agent for softening poultry meat

Also Published As

Publication number Publication date
JPWO2006100838A1 (en) 2008-08-28
JP4945762B2 (en) 2012-06-06
TW200700017A (en) 2007-01-01

Similar Documents

Publication Publication Date Title
JP5764927B2 (en) Animal feed additive and animal feed composition
Awad et al. Response of broilers to reduced-protein diets under heat stress conditions
JP4945762B2 (en) Method for increasing the amount of free glutamic acid in meat and its feed
RU2638033C2 (en) Fodder product from poultry feathers
RU2700094C1 (en) Fodder product for broiler chickens
RU2573960C2 (en) Feed product for broiler chickens
Koshchaev et al. Various sources of methionine in broiler chicken rations
RU2655843C1 (en) New additive to the diet and method of feeding pigs for fattening
Goswami et al. Growth and nutrient utilization in calves fed guar (Cyamopsis tetragonoloba) meal replacing groundnut cake in concentrate with and without added sweetener and flavour
KR101438440B1 (en) Feedstuff for beef cattle and breeding method using the same
CN1568746A (en) Alkali type zinc chloride feedstuff additive and preparation method thereof
RU2679058C1 (en) Combined feed for laying hens
RU2202896C2 (en) Feed for dogs and fur animals
RU2321268C2 (en) Fodder for broiler chickens
RU2599618C1 (en) Protein fodder product (versions)
JP4491527B2 (en) Method for increasing the amount of free glutamic acid in meat and feed therefor
JP2020162450A (en) Method for increasing free glutamic acid content in meat, and method for improving water holding capacity of meat
RU2802227C1 (en) Compound feed for broiler chickens
RU2729387C1 (en) Fodder additive for young sheep
JP5260101B2 (en) Method for suppressing increase of free glutamic acid in meat and acidity of meat
RU2706547C1 (en) Soya-betaine functional additive for pigs
RU2681795C1 (en) Compound feed for broiler chickens
Lisnahan et al. Effect of l-arginine and l-lysine supplementation in low-protein feeds on the growth of native chickens in the starter phase: https://doi. org/10.12982/VIS. 2023.061
JP2006158350A (en) Method for increasing free glutamic acid volume in meat of poultry and livestock, and feed for the same
KR0178297B1 (en) A feed additive of l-carnitine

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application
DPE2 Request for preliminary examination filed before expiration of 19th month from priority date (pct application filed from 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2007509158

Country of ref document: JP

NENP Non-entry into the national phase

Ref country code: DE

NENP Non-entry into the national phase

Ref country code: RU

WWW Wipo information: withdrawn in national office

Country of ref document: RU

122 Ep: pct application non-entry in european phase

Ref document number: 06712971

Country of ref document: EP

Kind code of ref document: A1

WWW Wipo information: withdrawn in national office

Ref document number: 6712971

Country of ref document: EP