RU2599618C1 - Protein fodder product (versions) - Google Patents

Abstract

FIELD: animal breeding.

SUBSTANCE: invention relates to fodder production, specifically to production of a protein fodder product (versions). Protein fodder product includes a corn extract and biologically active substance wastes of food industry, containing digestible protein and hydrolysis products, as well as contains lysine hydrochloride and tryptophan. As biologically active substances wastes from food industry, containing digestible protein and hydrolysis products, fodder product contains fermentolysate of soya bean meal or alkaline hydrolysate of keratin material, or fermentolysate of soya cake and alkaline hydrolysate of keratin material, taken in equal proportions. All components are taken in a certain proportion. Protein fodder products contain a large amount of easily digestible protein, peptide, amino acids.

EFFECT: feeding a fodder product provides high average daily weight gain of animals.

3 cl, 6 tbl

Description

Embodiments of the invention relate to the field of satisfying human vital needs and can be used in the manufacture of a protein feed product for animal husbandry.

Protein in feed in modern industrial animal husbandry is of particular importance, since it is by its content and the usefulness of the amino acid composition that the daily weight gain of animals and the period when commodity properties are achieved are determined.

The standard of feed protein is considered fishmeal. The protein of fish meal has the most optimal amino acid composition. The disadvantages of fishmeal are associated with its scarcity, high cost, short shelf life and strong fish smell. The latter property does not allow the use of fishmeal in the final stages of growing meat breeds of livestock and in dairy farming.

A promising modern trend is the development and integrated use of standardized high-quality protein feeds, which may also include unused or partially used waste from the food industry. Such waste includes corn extract, which is obtained as a by-product in the production of corn starch, confectionery molasses and crystalline glucose.

Corn extract is an aqueous extraction mainly from the embryo of corn grain and, after concentration, contains more than 30% of vegetable protein similar in gluten to amino acids, vitamins, amino acids, carbohydrates, minerals and growth stimulants of various classes of substances [Starch and starch products technology. / ed. Tregubova N.N., 5th ed. - M .; 1981, 472 pp.].

Currently, corn extract is of limited use as a stimulator of bacterial growth in industrial and laboratory microbiology, and a small amount of it is used in the preparation of corn feed. The wider use of corn extract, corresponding to its biologically active properties, is hindered by high acidity (pH about 4), difficulties in transportation in an evaporated form.

A known method of spray drying one stripped off corn extract [Technology of starch and starch products. / ed. Tregubova N.N., 5th ed. - M .; 472 p.]. However, due to the hygroscopicity of the resulting product, this method has not found application on an industrial scale.

It is also known to obtain a feed product using a technical solution set forth in USSR author's certificate No. 1085583, using acidic or enzymatic hydrolysis of a protein to convert it into a more digestible form. At the same time, the amino acid composition of the protein does not undergo changes and losses of the protein and amino acids and other biologically active substances due to thermal and chemical destruction and additional mineralization are added to the disadvantages of the commercial product.

The closest in technical essence is a protein-starch product obtained by the method described in RF Patent No. 2150213. The resulting complex feed includes about 60% protein, up to 25% starch, a small amount of fat. The production method involves mixing corn extract and gluten waste, reducing acidity, evaporation and spray drying. The disadvantage of the resulting product is that the amino acid composition of the protein does not differ from the amino acid composition of the protein of the corn extract and corresponds to the composition of gluten, which is deficient in sulfur-containing amino acids, tryptophan, and other essential and non-essential amino acids.

The objective of the invention is to eliminate this drawback by creating a protein feed product with a high content of easily digestible protein, peptides, amino acids, which is close in amino acid composition and physiological value to the standard of feed protein, fish meal.

The technical result that can be obtained by implementing the invention is to increase the bioefficiency of a protein feed product.

The solution of the problem and the achievement of the technical result became possible as a result of the development of the composition of the new protein feed product due to the fact that in the well-known protein product, including corn extract and containing biologically active substances of food industry waste:

- in the first embodiment, a protein feed product, including corn extract and biologically active food waste products containing digestible protein and products of its hydrolysis, as biologically active food waste products containing easily digestible protein and products of its hydrolysis, the feed product contains soybean meal fermentolizate and also contains lysine hydrochloride and tryptophan in the following ratio of ingredients,%: soybean meal fermentolizate - 50; corn extract - 45.6; lysine hydrochloride - 4.0; tryptophan - 0.4.

- in the second embodiment, a protein feed product, including corn extract and biologically active food waste products containing digestible protein and products of its hydrolysis, as biologically active food waste products containing easily digestible protein and products of its hydrolysis, the feed product contains an alkaline keratin hydrolyzate raw materials, and also contains lysine hydrochloride and tryptophan in the following ratio of ingredients,%: alkaline hydrolyzate of keratin raw materials - 50; corn extract - 46.5; lysine hydrochloride - 4; tryptophan - 0.4.

- in the third embodiment, a protein feed product, including corn extract and biologically active food waste products containing digestible protein and products of its hydrolysis, as biologically active food waste products containing easily digestible protein and products of its hydrolysis, the feed product contains soybean meal fermentolizate and keratin hydrolyzate, taken in equal proportions, and also contains lysine hydrochloride and tryptophan in the following ratio of ingredients,% : alkaline hydrolyzate of keratin raw materials - 25; soybean meal fermentolizate - 25; corn extract - 45.6; lysine hydrochloride - 4.0; tryptophan - 0.4.

An inventive step is the combination of an enzymatic hydrolyzate of soybean meal with corn extract (first option), a product of partial hydrolysis of keratin raw materials with corn extract (second option) or a mixture of these hydrolysates in equal proportions (third option), which allows to obtain a more complete protein product that is close in amino acid composition and stimulation of growth properties to the standard of feed protein, fish meal.

The lack of lysine and tryptophan for all variants of the composition of the feed protein product is eliminated by the additional introduction of small amounts of pure amino acids. The amounts of introduced amino acids are determined from the known standards for their content in the feed protein [Norms and diets of feeding farm animals. Reference manual, 3 revised and supplemented edition / ed. Kalashnikova A.P., Fisinina V.I. and others - M., 2003], and after additional administration of amino acids, they make up the physiological norm together with the available amount of these amino acids in hydrolysates and corn extract (from 7 to 8% in the protein for lysine and about 1% in the protein for tryptophan).

Thus, the disadvantages of the known protein product in the closest analogue are overcome and high bioefficiency of the newly developed product is achieved.

The practical applicability of the claimed variants of the protein feed product is illustrated by examples for different groups of animals and tables in which the data of the examples are summarized.

Table 1. Example 1. The composition of the main diet and feed additives used in experiments on weaned pigs.

Table 2. Example 1. Test results of feed additives on weaned piglets.

Table 3. Example 1. Biochemical blood parameters of weaned piglets.

Table 4. Example 2. Options for feed additives for experiments on broiler chickens.

Table 5. Example 2. Test results of the inventive feed additives on broiler chickens.

Table 6. Example 3. Change in dog weight after application of feed additives.

According to the first embodiment of the claimed composition, the protein feed product is obtained as follows.

A soybean meal fermentolizate is prepared by maintaining a suspension of soybean meal in water in the presence of the chymotrypsin enzyme at pH 7.5-8.0 for 3 hours. After separation of the coarse particles, the filtrate is evaporated to 25-35% solids, acidified with a corn extract with a dry solids concentration 45-50% to a pH of 4.5-5.5. Lysine hydrochloride is added to the resulting mass at the rate of 4% by weight of solids, 0.4% tryptophan to the mass of solids and is sent to spray drying. The result is a protein feed product with the following ratio of ingredients,%:

- fermentolizate of soybean meal - 50;

- corn extract - 45.6;

- lysine hydrochloride - 4.0;

- tryptophan - 0.4.

According to the second variant of the claimed composition, a protein feed product is obtained by replacing a soybean meal fermentolizate with a keratin feed hydrolyzate.

A keratin feed hydrolyzate is prepared in a separate reactor, mixing ground keratin feed with a 2% sodium hydroxide solution and hydrolyzing for 1 h at 90-100 ° C. Coarse particles are separated, the mixture is evaporated to 25-35% by dry matter, acidified with corn extract with a solids concentration of 45-50% to a pH of 4.5-5.5. Lysine hydrochloride is added to the resulting mass at the rate of 4% by weight of solids and 0.4% tryptophan to the mass of solids and is sent to spray drying. The result is a protein feed product with the following ratio of ingredients,%:

- hydrolyzate of keratin raw materials - 50;

- corn extract - 45.6;

- lysine hydrochloride - 4.0;

- tryptophan - 0.4.

According to the third variant of the claimed composition, a protein feed product is obtained by combining with a corn extract a mixture of soybean meal fermentolizate and keratin feed hydrolyzate, taken in equal proportions. To do this, get the soybean meal fermentolizate and keratin raw material hydrolyzate by the described methods and mix them in a 1: 1 ratio on dry matter, evaporate the mixture to 25-35% on dry matter, acidify the corn extract with a dry matter concentration of 45-50% to pH 4, 5-5.5. Lysine hydrochloride is added to the resulting mass at the rate of 4% by weight of solids and 0.4% tryptophan to the mass of solids and is sent to spray drying. The result is a protein feed product with the following ratio of ingredients,%:

- fermentolizate of soybean meal - 25;

- hydrolyzed keratin raw materials - 25;

- corn extract - 45.6;

- lysine hydrochloride - 4.0;

- tryptophan - 0.4.

The practical applicability of the protein feed product according to the first version of the composition is shown in example 1 of feeding weaned piglets (experiments 4 to 6 of tables 1, 2, 3) and example 2 when used on broiler chickens (experiments 10 to 12 of tables 4, 5) .

The practical applicability of the protein feed product according to the second version of the composition is shown in example 2, experiments 16 to 18 on broiler chickens and example 3 for feeding service dogs.

The practical applicability of the protein feed product according to the third embodiment is shown in Example 2, experiments 13 to 15 on broiler chickens.

Example 1. Conducting experiments on weaned pigs.

For carrying out experiments, groups of weaning pigs of 43 days old were formed.

Example 1. Experience 1.

The control group received the total diet (protein portion of the diet,% in the feed: soybean - 12; peas - 6; fish meal - 4, soybean meal - 2.0. The crude protein content in the feed - 12.2%, lysine - 5.7 %; tryptophan - 0.64%.

The period of the experiments, days - 20;

Case, goals - 1;

The average daily gain, g - 84.0;

Feed costs per 1 kg of weight gain, 1.45.

Blood biochemical parameters (initial data): total protein - 57.34 ± 1.30 g / l; calcium - 1.74 ± 0.52 mmol / l; phosphorus - 1.83 ± 0.37 mmol / l; cholesterol - 4.38 ± 0.87 mmol / l.

Blood biochemical parameters (final data): total protein - 57.70 ± 2.48 g / l; calcium - 1.88 ± 0.63 mmol / l; phosphorus - 1.96 ± 0.29 mmol / l; cholesterol - 4.42 ± 0.70 mmol / L.

The protein content in the blood of animals is at the lower limit of normal (normal from 58 to 89 g / l) and does not change after the experiment.

Violations in the digestion and absorption of protein and lipids are also evidenced by a violation of calcium-phosphorus balance (the ratio of calcium to phosphorus is 1.26 instead of 1.5-2.0 normal) and cholesterol levels located at the upper limit of the norm (from 3.5 to 5.25 mmol / L with a norm of up to 5.2 mmol / L).

Example 1. Experience 2.

The group received 15% protein feed product - the closest analogue to replace all sources of protein in the feed. The crude protein content of 12.2%, lysine - 2.7%; tryptophan - 0.6%.

The period of the experiments, days - 20;

Case, goals - absent;

The average daily gain, g - 82.6 (-1.7% relative to the control);

Feed costs per 1 kg of weight gain, kg - 1.5 (+ 3.4% relative to the control).

Blood biochemical parameters (initial data): total protein - 57.24 ± 1.22 g / l; calcium - 1.78 ± 0.42 mmol / l; phosphorus - 1.88 ± 0.35 mmol / l; cholesterol - 4.52 ± 0.74 mmol / L.

Blood biochemical parameters (final data): total protein - 47.26 ± 2.52 g / l; calcium - 1.70 ± 0.45 mmol / l; phosphorus - 2.30 ± 0.40 mmol / l; cholesterol 1.94 ± 0.77 mmol / L.

Example 1. Experience 3.

The group received 15% of fishmeal instead of all protein sources. The crude protein content in the feed is 12.2%, lysine - 8%; tryptophan - 1%.

The period of the experiments, days - 20;

Case, goals - 1;

The average daily gain, g - 102.4 (+ 21.9%) relative to the control);

The cost of feed per 1 kg of weight gain, kg - 1.28 (-11.7% relative to the control).

Blood biochemical parameters (initial data): total protein - 57.9 ± 1.40 g / l; calcium - 1.80 ± 0.51 mmol / l; phosphorus - 1.8 ± 0.3 5 mmol / l; cholesterol - 4.61 ± 0.83 mmol / L.

Blood biochemical parameters (final data): total protein - 69.73 ± 2.55 g / l; calcium - 2.10 ± 0.47 mmol / l; phosphorus - 1.90 ± 0.55 mmol / l; cholesterol 1.56 ± 0.76 mmol / L.

Example 1. Experience 4.

In the first experimental group (the first paragraph of the formula), all protein ingredients were replaced by 15% protein feed product of the following composition: 40% soybean meal fermentolizate; 55.6% corn extract; 4% lysine hydrochloride; 0.4% tryptophan, which corresponds to an underestimated value of the content of soybean meal fermentolizate relative to the claimed and overestimated content of corn extract. Crude protein content in the feed - 12.2%; lysine hydrochloride - 4% (total lysine in the protein 7.4%); tryptophan - 0.4% (total tryptophan in protein 1.1%).

The period of the experiments, days - 20;

Case, goals - absent;

The average daily gain, g - 101.8 (+ 21.2% to control);

The cost of feed per 1 kg of weight gain is 1.3 (-10.3% relative to the control).

Blood biochemical parameters (initial data): protein - 57.20 ± 1.40 g / l; calcium - 1.75 ± 0.47 mmol / l; phosphorus - 1.90 ± 0.34 mmol / l; cholesterol - 4.45 ± 0.75 mmol / L.

Blood biochemical parameters (final data): protein 71.15 ± 2.30 g / l; calcium 2.60 ± 0.45 mmol / l; phosphorus - 1.79 ± 0.42 mmol / l; cholesterol - 1.8 ± 0.76 mmol / L.

Example 1. Experience 5.

In the second experimental group (the first paragraph of the formula), all protein ingredients were replaced by 15% protein feed product of the following composition: 50% soybean meal fermentolizate; 45.6% of corn extract; 4% lysine hydrochloride; 0.4% tryptophan, which corresponds to the declared value of the soybean meal fermentolizate and corn extract. Crude protein in feed 12.2%; lysine hydrochloride - 4% (total lysine in the protein is 7.0%); tryptophan 0.4% (total tryptophan in protein 1.0%).

The period of the experiments, days - 20;

Case, goals - absent;

Average daily gain, city - 102.1 (+ 21.5% relative to control);

The cost of feed per 1 kg of weight gain, kg - 1.3 (-10.3% relative to the control).

Blood biochemical parameters (initial data): total protein - 57.90 ± 1.46 g / l; calcium - 1.77 ± 0.45 mmol / l; phosphorus - 1.80 ± 0.34 mmol / l; cholesterol 4.40 ± 0.60 mmol / L.

Blood biochemical parameters (final data): total protein - 70.26 ± 2.40 g / l; calcium - 2.54 ± 0.56 mmol / l; phosphorus - 1.82 ± 0.40 mmol / l; cholesterol - 1.67 ± 0.76 mmol / l.

Example 1. Experience 6.

In the third experimental group (the first paragraph of the formula), all protein ingredients were replaced with 15% of the protein feed product of the following composition: 60% of soybean meal fermentolizate; 35.6% corn extract; 4% lysine hydrochloride; 0.4% tryptophan, which corresponds to an overestimated content of soybean meal fermentolizate with respect to the claimed and underestimated content of corn extract. Crude protein 12.2% in feed; lysine hydrochloride - 4% (total lysine in the protein 7.4%); tryptophan 0.4% (total tryptophan in protein 1.1%).

The period of the experiments, days - 20;

Case, goals - 1;

The average daily gain, g. - 101.0 (+ 20.2% relative to control);

Feed costs per 1 kg of weight gain, kg - 1.32 (-9.0% relative to the control).

Blood biochemical parameters (initial data): total protein - 57.30 ± 1.20 g / l; calcium - 1.80 ± 0.35 mmol / l; phosphorus - 1.90 ± 0.35 mmol / l; cholesterol 4.55 ± 0.56 mmol / L.

Blood biochemical parameters (final data): total protein - 70.35 ± 2.45; calcium - 2.47 ± 0.55 mmol / l; phosphorus - 1.85 ± 0.35 mmol / l; cholesterol - 1.82 ± 0.56 mmol / l.

The hydrolyzate of keratin raw materials was not introduced into the studied compositions due to the undesirability of using animal protein sources (except fishmeal and milk waste) in feeding piglets.

According to the results of the experiments of Example 1, it can be seen that in the case of the use of a feed additive, the closest analogue, the technical and economic indicators were lower both in comparison with fishmeal, the claimed protein feed product, and in comparison with the general diet on the farm. The influence of the inventive feed additive was slightly inferior to the effect of fish meal in weight gain and specific feed consumption, but had the advantage of affecting protein levels and calcium-phosphorus balance. In the blood of animals in groups with fishmeal and the claimed feed product (experiments 3-6), the protein content statistically significantly increased. In experiments 1 and 2, such trends were not detected.

An improvement in metabolic processes in the animal organism is also evidenced by an increase in the content of calcium and phosphorus in the blood of animals (experiments 3-6) with normalization of calcium-phosphorus balance: from 1.2-1.25 at the beginning of the experiment to 1.8-1.7 per stage of completion. Moreover, in the control experiment, this ratio was 1.23 at the beginning and end of the experiment, and in experiment 2 with the closest analogue it decreased from 1, 22 to 1.0.

A positive effect on lipid metabolism is illustrated by a decrease to the physiological norm (less than 5.2 mmol / l) of cholesterol in experiments with the claimed protein feed product and fish meal.

For other biochemical blood parameters, no significant changes were detected.

Taking into account the tendency to worsen the positive effect of the protein feed product in experiment 6 with an increased content of soybean meal fermentolizate, as well as economic considerations: the cost of soybean meal fermentolizate is 10-15 times higher than the cost of corn extract, an increase in the relative content of soybean meal enzyme more than 50% are deemed inappropriate.

Shifts towards an increase in the relative content of corn extract (experiment 4) influenced the technological process of manufacturing a protein feed product: spray drying was complicated and the resulting product had increased hygroscopicity and reduced shelf life.

Example 2. The use of protein feed product in broiler chickens.

In experiments on broiler chickens, 12 groups of animals of 20 days of age were formed, 80 animals each. The duration of the experiment is 15 days.

Example 2. Experience 7.

The total diet consisted of starter feed, taken on the farm,%: protein - 20; lysine in protein - 5.4; tryptophan in protein - 1.1.

The number of goals at the end - 75; safety - 93.8%; the average weight gain per day is 51.2 g; feed costs per 1 kg of weight gain - 1.8 kg.

Example 2. Experience 8.

The group received food, in which 10% of the starting feed was replaced by 6% of the protein feed product according to the closest analogue,%: corn extract - 50; - the rest is the dry residue of gluten waste and 4% of the grain mixture. Protein -20; lysine in protein - 2.7; tryptophan in protein - 0.6.

The number of goals at the end - 79; safety - 98.8%; the average weight gain per day is 48.5 g (-5.3% relative to the control) .; feed costs per 1 kg of weight gain - 2.2 kg (+ 22.2% relative to control).

Example 2. Experience 9. The group received food, in which 10% of the starting feed was replaced by 6% of fish meal and 4% of grain mix. Protein - 20%; lysine in protein - 8.0%; tryptophan in protein - 1.0%.

The number of goals at the end - 78; safety - 97.5%; the average weight gain per day is 52.3 g (+ 2.1% relative to the control) .; feed costs per 1 kg of weight gain - 1.75 kg (-1.1% relative to control).

Example 2. experiment 10.

The group received feed, in which 10% of the starting feed was replaced by 6% of the protein feed product (40% of soybean meal fermentolizate; 55.6% of corn extract; lysine hydrochloride - 4%; tryptophan - 0.4%, 4% of grain mix). Protein - 20%; lysine in protein - 7.50%; tryptophan in protein - 1.15%.

The number of goals at the end - 79; safety - 98.8%; the average weight gain per day is 53.0 g (+ 3.5% relative to the control) .; feed costs per 1 kg of weight gain - 1.75 kg (-2.8% relative to control).

Example 2. Experience 11.

The group received feed, in which 10% of the starting feed was replaced by 6% of the protein feed product (50% of soybean meal fermentolizate; 45.6%; corn extract; lysine hydrochloride - 4%; tryptophan - 0.4%; 4% grain mix). Protein - 20%; lysine in protein - 7.4%; tryptophan in protein - 1.2%.

The number of goals at the end - 79; safety - 98.8%; the average weight gain per day is 53.5 g (+ 4.5% relative to the control) .; feed costs per 1 kg of weight gain - 1.75 kg (-2.8% relative to control).

Example 2. Experience 12.

The group received food, in which 10% of the starting feed was replaced by 6% of the protein feed product (60% of soybean meal fermentolizate; 35.6% of corn extract; lysine hydrochloride - 4%; tryptophan - 0.4%; 4% grain mix). Protein - 20%; lysine in protein - 7.50%; tryptophan in protein - 1.1%.

The number of goals at the end - 79; safety - 98.8%; the average weight gain per day is 53.0 g (+ 3.5% relative to the control) .; feed costs per 1 kg of weight gain - 1.75 kg (-2.8%) relative to the control).

From example 2 (experiment 8) it is seen that the introduction of the feed additive of the closest analogue worsened the technical and economic indicators of broiler chickens: there was a decrease in daily weight gain and the associated increased feed consumption compared to control (example 2, experiment 7). In experiments with the claimed feed additives, the technical and economic indicators were comparable with similar indicators in the experiment with fish meal. An increase in the content of soybean meal fermentolizate in the claimed feed product by 10%) (example 2, experiment 12) did not lead to statistically significant changes in consumer properties.

The increase in the content of the corn extract in the feed product (example 2, experiment 10) due to the decrease in the amount of the introduced fermentolizate worsened the technological and consumer properties: there were difficulties in spray drying, hygroscopicity and caking of the product increased.

A further increase in the relative content of corn extract led to a decrease in the protein concentration in the feed additive and exacerbated the problems encountered during drying and storage: the product could not be dried by spray drying, and with other types of drying, a product with increased hygroscopicity and reduced shelf life was obtained.

In experiments 16 to 18 of example 2 and the experience of example 3, the practical applicability of the second variant of the claimed composition of the protein feed product was evaluated.

Example 2. Experience 16.

The group received feed, in which 10% of the starting feed was replaced with 6% of the protein feed product (40% of the keratin feed alkaline hydrolyzate; 55.6% of the corn extract; lysine hydrochloride - 4%; tryptophan - 0.4%; 4% grain mix). Protein - 20%; lysine in protein - 7.2%; tryptophan in protein - 0.9%.

The number of goals at the end - 79; safety - 98.8%; the average weight gain per day is 52.0 g (+ 1.6% relative to the control) .; feed costs per 1 kg of weight gain - 1.77 kg (-1.7% relative to control).

Example 2. Experience 17.

The group received feed in which 10% of the starting feed was replaced with 6% of the protein feed product (50% of the alkaline hydrolyzate of keratin raw materials; 45.6% of corn extract; lysine hydrochloride - 4%; tryptophan - 0.4%; 4% of the grain mixture). Protein - 20%; lysine in protein - 7.5%; tryptophan in protein - 1.1%.

The number of goals at the end - 79; safety - 98.8%; the average weight gain per day is 53.1 g (+3, 7% relative to the control) .; feed costs per 1 kg of weight gain - 1.75 kg (-2.8%) relative to the control).

Example 2. Experience 18.

The group received food, in which 10% of the starting feed was replaced by 6% of the protein feed product (60% of the alkaline hydrolyzate of keratin raw materials; 35.6% of corn extract; lysine hydrochloride - 4%; tryptophan - 0.4%; 4% of grain mix). Protein - 20%; lysine in protein - 7.6%; tryptophan in protein - 1.1%.

The number of goals at the end - 79; safety - 98.8%; the average weight gain per day is 52.8 g (+3, 1% relative to the control) .; feed costs per 1 kg of weight gain - 1.78 kg (-1.1%) relative to the control).

When using the inventive protein feed product according to the second version of the composition, results were obtained that are not inferior to the results of the experiment with fish meal.

A 10% increase in the content of keratin hydrolyzate did not lead to a significant change in the results on weight gain and specific feed consumption, but increased the cost of the target product, while a decrease with a simultaneous increase in the relative content of the corn extract worsened consumer properties: hygroscopicity and reduced shelf life increased.

Example 3. The use of the inventive feed additives on service dogs. Due to the fact that the feed additive, the closest analogue, is not intended for feeding unproductive animals, namely dogs, the effectiveness of the claimed feed additives for dogs was estimated by the increase in live weight in the autumn-winter period with a decrease in air temperature and increased muscle loads, and also when recovering after weaning puppies or past diseases in comparison with fishmeal.

The first control group included 5 German shepherds without signs of illness and 1 dog after weaning puppies. They were fed, in addition to the diet, 15 g of fishmeal for 40 days.

In the second experimental group, along with three clinically healthy dogs, there was a dog after suffering pyroplasmosis and one dog after weaning puppies. They received 15 g of the claimed protein feed additives (composition of experiment 17 of table 5) for 40 days. The results of the experiments are shown in table 6.

The weight gain of the animals testified to the normalization of protein-mineral metabolism both in the case of fishmeal and when using the inventive feed additive. In the experimental group, gains in live weight, improvement in coat and appearance of dogs were more pronounced.

The results of experiments 13, 14, 15 of example 2 illustrate the applicability of the third, independent variant of the composition of the protein feed product on broiler chickens.

Example 2. Experience 13.

The group received feed in which 10% of the feed was replaced with 6% of the protein feed product (25% soybean meal enzyme lysate; 25% keratin feed alkaline hydrolyzate; 45.6% corn extract; lysine hydrochloride 4%; tryptophan 0.4% ; 4% grain mix). Protein - 20%; lysine in protein - 7.5%; tryptophan in protein - 1.1%.

The number of goals at the end - 79; safety - 98.8%; the average weight gain per day is 53.2 g (+3, 9% relative to the control) .; feed costs per 1 kg of weight gain - 1.75 kg (-2.8%) relative to the control).

Example 2. Experience 14.

The group received feed in which 10% of the feed was replaced with 6% of the protein feed product (35% soybean meal fermentolizate; 15% keratin feed alkaline hydrolyzate; 45.6% corn extract; lysine hydrochloride 4%; tryptophan 0.4% ; 4% grain mix). Protein - 20%; lysine in protein - 7.7%; tryptophan in protein - 1.16%.

The number of goals at the end - 78; safety - 97.5%; the average weight gain per day is 52.8 g (+3, 1% relative to the control) .; feed costs per 1 kg of weight gain - 1.76 kg (-2.2%) relative to the control).

Example 2. Experience 15.

The group received feed in which 10% of the feed was replaced with 6% of the protein feed product (15% of soybean meal fermentolizate; 35% of keratin feed alkaline hydrolyzate; 45.6% of corn extract; lysine hydrochloride - 4%; tryptophan - 0.4% ; 4% grain mix). Protein - 20%; lysine in protein - 7.5%; tryptophan in protein - 1.1%.

The number of goals at the end - 79; safety - 98.8%; the average weight gain per day is 52.8 g (+ 3.1% relative to the control) .; feed costs per 1 kg of weight gain - 1.756 g (-2,% relative to control).

Using a combination of soybean meal fermentolizate and keratin raw alkaline hydrolyzate is effective, and changing their relative contents by 10% in one direction or another does not have a statistically significant effect on the experimental results, and the choice of a combination of these ingredients should be made for reasons of economic feasibility: the cost of soybean meal enzyme lysate higher than the cost of an alkaline hydrolyzate of keratin raw materials, or preferences in animal feeding: for pigs and cattle, the introduction of protein into diets is lively Foot origin (except for fish meal and milk processing) is not desirable, while for poultry use of animal protein non-avian origin are allowed.

The above examples of the use of the inventive protein feed product indicate high bioefficiency, comparable with the bioeffectiveness of fish meal and significantly higher than the bioeffectiveness of the closest analogue. In terms of shelf life, the absence of a characteristic odor that can be transmitted to marketable livestock products, and the scarcity, the new protein feed product has advantages over the standard of feed protein, fish meal.

Thus, the claimed combination of essential features of the variants of the invention allows to solve the problem and significantly increase the bioefficiency of the feed product in comparison with the closest analogue.

Claims (3)

1. Protein feed product, comprising corn extract and biologically active food waste products containing digestible protein and products of its hydrolysis, characterized in that as biologically active food waste products containing easily digestible protein and products of its hydrolysis, the feed product contains fermentolizate soybean meal, and also contains lysine hydrochloride and tryptophan in the following ratio of ingredients,%: soybean meal fermentolizate - 50; corn extract - 45.6; lysine hydrochloride - 4.0; tryptophan - 0.4. 2. Protein feed product, including corn extract and biologically active food waste products containing digestible protein and its hydrolysis products, characterized in that as biologically active food waste products containing easily digestible protein and its hydrolysis products, the feed product contains alkaline hydrolyzed keratin raw materials, and also contains lysine hydrochloride and tryptophan in the following ratio of ingredients,%: alkaline hydrolyzed keratin raw materials - 50; corn extract - 46.5; lysine hydrochloride - 4; tryptophan - 0.4. 3. Protein feed product, including corn extract and biologically active food waste products containing digestible protein and products of its hydrolysis, characterized in that as biologically active food waste products containing easily digestible protein and products of its hydrolysis, the feed product contains fermentolizate soybean meal and alkaline hydrolyzate of keratin raw materials, taken in equal proportions, and also contains lysine hydrochloride and tryptophan in the following ratio in ingredients,%: alkaline hydrolyzate of keratin raw materials - 25; soybean meal fermentolizate - 25; corn extract - 45.6; lysine hydrochloride - 4.0; tryptophan - 0.4.